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Conserved domains on  [gi|1519314609|ref|NP_060909|]
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protein IMPACT [Homo sapiens]

Protein Classification

IMPACT family protein( domain architecture ID 10651914)

IMPACT (imprinted ancient) family protein similar to Homo sapiens protein IMPACT, which is a translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation

CATH:  3.10.110.10
Gene Ontology:  GO:0034198|GO:0140311|GO:0006446|GO:0005515
PubMed:  11116084|24333428|26495453|26276859|25918163

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RWD_IMPACT cd23821
RWD domain of protein IMPACT and related proteins; IMPACT, also imprinted and ancient gene ...
 13-113 6.58e-44

RWD domain of protein IMPACT and related proteins; IMPACT, also imprinted and ancient gene protein homolog, acts as a translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment, and glucose deprivation. It plays a role as a negative regulator of EIF2AK4/GCN2 kinase activity. It impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. IMPACT may be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, IMPACT plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.


:

Pssm-ID: 467657  Cd Length: 101  Bit Score: 145.84  E-value: 6.58e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  13 NEEIEAMAAIYGEEWCVIDDCAKIFCIRISDDIDdPKWTLCLQVMLPNEYPGTAPPIYQLNAPWLKGQERADLSNSLEEI 92
Cdd:cd23821     1 AEEIEALEAIYGEDFVVIDESARSFVIRIELDGP-HLPPLVLRVHLPPDYPSHSPPIFELSAPWLSGEERSELCAELDEI 79

                          90       100
                  ....*....|....*....|.
gi 1519314609  93 YIQNIGESILYLWVEKIRDVL 113
Cdd:cd23821    80 WEENAGEPVLFQWVEWLREYL 100
UPF0029 pfam01205
Uncharacterized protein family UPF0029;
182-289 1.39e-43

Uncharacterized protein family UPF0029;


:

Pssm-ID: 460111  Cd Length: 105  Bit Score: 144.87  E-value: 1.39e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609 182 RRSTFQAHLAPVVCPKQVKMVLSKL-YENKKiasATHNIYAYRIYCEDKQtflqDCEDDGETA--AGGRLLHLMEILNVK 258
Cdd:pfam01205   1 KKSKFIAHAAPVESEEEAKAFLEELkKEHKK---ATHNCYAYRIGGEGGE----RSSDDGEPGgtAGKPILEVLEGNGLT 73

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1519314609 259 NVMVVVSRWYGGILLGPDRF-KHINNCARNIL 289
Cdd:pfam01205  74 NVLVVVTRYFGGIKLGPGGLvRAYSNAAREAL 105
 
Name Accession Description Interval E-value
RWD_IMPACT cd23821
RWD domain of protein IMPACT and related proteins; IMPACT, also imprinted and ancient gene ...
 13-113 6.58e-44

RWD domain of protein IMPACT and related proteins; IMPACT, also imprinted and ancient gene protein homolog, acts as a translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment, and glucose deprivation. It plays a role as a negative regulator of EIF2AK4/GCN2 kinase activity. It impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. IMPACT may be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, IMPACT plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.


Pssm-ID: 467657  Cd Length: 101  Bit Score: 145.84  E-value: 6.58e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  13 NEEIEAMAAIYGEEWCVIDDCAKIFCIRISDDIDdPKWTLCLQVMLPNEYPGTAPPIYQLNAPWLKGQERADLSNSLEEI 92
Cdd:cd23821     1 AEEIEALEAIYGEDFVVIDESARSFVIRIELDGP-HLPPLVLRVHLPPDYPSHSPPIFELSAPWLSGEERSELCAELDEI 79

                          90       100
                  ....*....|....*....|.
gi 1519314609  93 YIQNIGESILYLWVEKIRDVL 113
Cdd:cd23821    80 WEENAGEPVLFQWVEWLREYL 100
UPF0029 pfam01205
Uncharacterized protein family UPF0029;
182-289 1.39e-43

Uncharacterized protein family UPF0029;


Pssm-ID: 460111  Cd Length: 105  Bit Score: 144.87  E-value: 1.39e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609 182 RRSTFQAHLAPVVCPKQVKMVLSKL-YENKKiasATHNIYAYRIYCEDKQtflqDCEDDGETA--AGGRLLHLMEILNVK 258
Cdd:pfam01205   1 KKSKFIAHAAPVESEEEAKAFLEELkKEHKK---ATHNCYAYRIGGEGGE----RSSDDGEPGgtAGKPILEVLEGNGLT 73

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1519314609 259 NVMVVVSRWYGGILLGPDRF-KHINNCARNIL 289
Cdd:pfam01205  74 NVLVVVTRYFGGIKLGPGGLvRAYSNAAREAL 105
RWD smart00591
domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily ...
 15-115 2.91e-30

domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily related to the UBCc domain;


Pssm-ID: 214735  Cd Length: 107  Bit Score: 110.52  E-value: 2.91e-30
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609   15 EIEAMAAIYGEEWCVIDDCAKIFCIRI-----SDDIDDPKWTLCLQVMLPNEYPGTAPPIYQLNAPWLKGQERADLSNSL 89
Cdd:smart00591   1 ELEALESIYPEDFEVIDEDARIPEITIklspsSDEGEDQYVSLTLQVKLPENYPDEAPPISLLNSEGLSDEQLAELLKKL 80

                           90       100
                   ....*....|....*....|....*.
gi 1519314609   90 EEIYIQNIGESILYLWVEKIRDVLIQ 115
Cdd:smart00591  81 EEIAEENLGEVMIFELVEKLQEFLSE 106
RWD pfam05773
RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. ...
 10-113 1.02e-21

RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices.


Pssm-ID: 399058  Cd Length: 111  Bit Score: 88.15  E-value: 1.02e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  10 QRQNEEIEAMAAIYGEEWCVIDD-CAKIFCIRISDDID------DPKWTLCLQVMLPNEYPgTAPPIYQLNAPW-LKGQE 81
Cdd:pfam05773   1 EEQEEELEALESIYPDEFEVISDsPYESLEIEIKLSLDsdesdsSHLPPLVLKFTLPEDYP-DEPPKISLSSPWnLSDEQ 79

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1519314609  82 RADLSNSLEEIYIQNIGESILYLWVEKIRDVL 113
Cdd:pfam05773  80 VLSLLEELEELAEENLGEVMIFELIEWLQENL 111
YIH1 COG1739
Putative translation regulator, IMPACT (imprinted ancient) protein family [General function ...
 179-274 3.15e-16

Putative translation regulator, IMPACT (imprinted ancient) protein family [General function prediction only];


Pssm-ID: 441345 [Multi-domain]  Cd Length: 198  Bit Score: 75.52  E-value: 3.15e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609 179 ITDRRSTFQAHLAPVVCPKQVKMVLSKLyeNKKIASATHNIYAYRIYCEDKQtflQDCEDDGE---TAagGR-LLHLMEI 254
Cdd:COG1739    15 IEIKKSRFIAYAAPVESEEEAKAFIAEI--RKEHPDATHNCWAYRIGAPGEI---QRASDDGEpsgTA--GKpILEVLQG 87

                          90       100
                  ....*....|....*....|
gi 1519314609 255 LNVKNVMVVVSRWYGGILLG 274
Cdd:COG1739    88 RGLTNVLVVVTRYFGGIKLG 107
 
Name Accession Description Interval E-value
RWD_IMPACT cd23821
RWD domain of protein IMPACT and related proteins; IMPACT, also imprinted and ancient gene ...
 13-113 6.58e-44

RWD domain of protein IMPACT and related proteins; IMPACT, also imprinted and ancient gene protein homolog, acts as a translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment, and glucose deprivation. It plays a role as a negative regulator of EIF2AK4/GCN2 kinase activity. It impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. IMPACT may be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, IMPACT plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.


Pssm-ID: 467657  Cd Length: 101  Bit Score: 145.84  E-value: 6.58e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  13 NEEIEAMAAIYGEEWCVIDDCAKIFCIRISDDIDdPKWTLCLQVMLPNEYPGTAPPIYQLNAPWLKGQERADLSNSLEEI 92
Cdd:cd23821     1 AEEIEALEAIYGEDFVVIDESARSFVIRIELDGP-HLPPLVLRVHLPPDYPSHSPPIFELSAPWLSGEERSELCAELDEI 79

                          90       100
                  ....*....|....*....|.
gi 1519314609  93 YIQNIGESILYLWVEKIRDVL 113
Cdd:cd23821    80 WEENAGEPVLFQWVEWLREYL 100
UPF0029 pfam01205
Uncharacterized protein family UPF0029;
182-289 1.39e-43

Uncharacterized protein family UPF0029;


Pssm-ID: 460111  Cd Length: 105  Bit Score: 144.87  E-value: 1.39e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609 182 RRSTFQAHLAPVVCPKQVKMVLSKL-YENKKiasATHNIYAYRIYCEDKQtflqDCEDDGETA--AGGRLLHLMEILNVK 258
Cdd:pfam01205   1 KKSKFIAHAAPVESEEEAKAFLEELkKEHKK---ATHNCYAYRIGGEGGE----RSSDDGEPGgtAGKPILEVLEGNGLT 73

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1519314609 259 NVMVVVSRWYGGILLGPDRF-KHINNCARNIL 289
Cdd:pfam01205  74 NVLVVVTRYFGGIKLGPGGLvRAYSNAAREAL 105
RWD smart00591
domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily ...
 15-115 2.91e-30

domain in RING finger and WD repeat containing proteins and DEXDc-like helicases subfamily related to the UBCc domain;


Pssm-ID: 214735  Cd Length: 107  Bit Score: 110.52  E-value: 2.91e-30
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609   15 EIEAMAAIYGEEWCVIDDCAKIFCIRI-----SDDIDDPKWTLCLQVMLPNEYPGTAPPIYQLNAPWLKGQERADLSNSL 89
Cdd:smart00591   1 ELEALESIYPEDFEVIDEDARIPEITIklspsSDEGEDQYVSLTLQVKLPENYPDEAPPISLLNSEGLSDEQLAELLKKL 80

                           90       100
                   ....*....|....*....|....*.
gi 1519314609   90 EEIYIQNIGESILYLWVEKIRDVLIQ 115
Cdd:smart00591  81 EEIAEENLGEVMIFELVEKLQEFLSE 106
RWD pfam05773
RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. ...
 10-113 1.02e-21

RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices.


Pssm-ID: 399058  Cd Length: 111  Bit Score: 88.15  E-value: 1.02e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  10 QRQNEEIEAMAAIYGEEWCVIDD-CAKIFCIRISDDID------DPKWTLCLQVMLPNEYPgTAPPIYQLNAPW-LKGQE 81
Cdd:pfam05773   1 EEQEEELEALESIYPDEFEVISDsPYESLEIEIKLSLDsdesdsSHLPPLVLKFTLPEDYP-DEPPKISLSSPWnLSDEQ 79

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1519314609  82 RADLSNSLEEIYIQNIGESILYLWVEKIRDVL 113
Cdd:pfam05773  80 VLSLLEELEELAEENLGEVMIFELIEWLQENL 111
YIH1 COG1739
Putative translation regulator, IMPACT (imprinted ancient) protein family [General function ...
 179-274 3.15e-16

Putative translation regulator, IMPACT (imprinted ancient) protein family [General function prediction only];


Pssm-ID: 441345 [Multi-domain]  Cd Length: 198  Bit Score: 75.52  E-value: 3.15e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609 179 ITDRRSTFQAHLAPVVCPKQVKMVLSKLyeNKKIASATHNIYAYRIYCEDKQtflQDCEDDGE---TAagGR-LLHLMEI 254
Cdd:COG1739    15 IEIKKSRFIAYAAPVESEEEAKAFIAEI--RKEHPDATHNCWAYRIGAPGEI---QRASDDGEpsgTA--GKpILEVLQG 87

                          90       100
                  ....*....|....*....|
gi 1519314609 255 LNVKNVMVVVSRWYGGILLG 274
Cdd:COG1739    88 RGLTNVLVVVTRYFGGIKLG 107
RWD_RNF14 cd23820
RWD domain of RING finger protein 14 (RNF14) and related proteins; RNF14, also called androgen ...
 12-111 7.17e-15

RWD domain of RING finger protein 14 (RNF14) and related proteins; RNF14, also called androgen receptor (AR)-associated protein 54 (ARA54), HFB30, or Triad2 protein, is an RBR-type E3 ubiquitin-protein ligase (EC 2.3.2.31) that is highly expressed in the testis and interacts with class III E2s (UBE2E2, UbcH6, and UBE2E3). Its differential localization may play an important role in testicular development and spermatogenesis in humans. RNF14 functions as a transcriptional regulator of mitochondrial and immune function in muscles. It is a ligand-dependent AR co-activator that enhances AR-dependent transcriptional activation. It also may participate in enhancing cell cycle progression and cell proliferation via induction of cyclin D1. Moreover, RNF14 is crucial for colon cancer cell survival. It acts as a new enhancer of Wnt-dependent transcriptional outputs that act at the level of the T-cell factor/lymphoid enhancer factor (TCF/LEF)-beta-catenin complex.


Pssm-ID: 467656 [Multi-domain]  Cd Length: 125  Bit Score: 70.08  E-value: 7.17e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  12 QNEEIEAMAAIYGEEwCVIDDCAKIFCIRI---------------SDDIDDPKWTLCLQ--------VMLPNEYPGTAPP 68
Cdd:cd23820     1 QEDELEALEAIYPDD-LVVDSDSSSGRGSLeipvelepplsvvlsSDGSDEGERTLKVShlppitlrFSLPPGYPSTSPP 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 1519314609  69 IYQLNAPWLKGQERADLSNSLEEIYIQNIGESILYLWVEKIRD 111
Cdd:cd23820    80 EFTLECSWLSPEQLSALCERLDELWEENGGDVVLFSWIDFLQD 122
RWD_DRWD_ELF-like cd11605
RWD, DRWD, and ELF domain family; The family includes the RWD, double-RWD (DRWD), and ELF ...
 18-109 6.89e-13

RWD, DRWD, and ELF domain family; The family includes the RWD, double-RWD (DRWD), and ELF domains. They belong to the ubiquitin-conjugating (UBC) superfamily that represents a structural domain with an alpha-beta(4)-alpha(3) core fold. The RWD domain (named after three major RWD-containing proteins: RING finger, WD-repeat-containing proteins and DEXD-like helicases) mediates protein-protein interactions in a variety of pathways in eukaryotes. The DRWD domain is responsible for substrate binding. It is involved in interactions with other kinetochore proteins. The ELF (N-terminal E2-like fold) domain is found in all Fanconi anemia group L protein (FANCL) homologs. It is required to promote efficient DNA damage-induced FANCD2 (Fanconi anemia group D2 protein) monoubiquitination in vertebrate cells.


Pssm-ID: 467641  Cd Length: 94  Bit Score: 63.74  E-value: 6.89e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  18 AMAAIYGEEWCVIDDCAKI-FCIRISDDIDDPKWTLCLQVMLPNEYPGTAPPIYQLNAPWLKGQER-ADLSNSLEEIYIQ 95
Cdd:cd11605     1 ALESIYGDELEVLSDDSPLrFSIRLSPEEEEDDPPLELEFTLPPGYPPEEPPLITLRSPKLSSAERlSLLKLELEEAAEE 80

                          90
                  ....*....|....
gi 1519314609  96 NIGESILYLWVEKI 109
Cdd:cd11605    81 NLGEPMLFDLVEAL 94
RWD_RWDD3 cd23819
RWD domain of RWD domain-containing protein 3 (RWDD3) and related proteins; RWDD3, also called ...
 14-106 2.84e-09

RWD domain of RWD domain-containing protein 3 (RWDD3) and related proteins; RWDD3, also called RWD domain-containing sumoylation enhancer (RSUME), acts as an enhancer of SUMO conjugation and has no effect on ubiquitination. It increases protein sumoylation (a dynamic ubiquitin-like post translational modification) of several proteins including HIF1alpha and I-kappa-B, through direct interaction with UBC9. Its RWD domain is required for the sumoylation enhancement activity.


Pssm-ID: 467655  Cd Length: 106  Bit Score: 53.87  E-value: 2.84e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  14 EEIEAMAAIYGE--EWCVIDDCAKIFC----IRISDDIDDPKWTLCLQVMLPNEYPGTAPPIyQLNAPWLKGQERADLSN 87
Cdd:cd23819     1 DELSVLQAIFCGpgEFEVLSSSETSDGvsfkIQISVEGFDEDIVLKLTFHLSPNYPSSLPDI-SVSSEQLTRAQCNDLQD 79

                          90       100
                  ....*....|....*....|..
gi 1519314609  88 SLEEIYIQNIGESI---LYLWV 106
Cdd:cd23819    80 SLLEYANSLLGEPMvleLVLWL 101
RWD_GCN2 cd23823
RWD domain of eIF-2-alpha kinase GCN2 and related proteins; GCN2 (EC 2.7.11.1), also called ...
 10-113 7.13e-09

RWD domain of eIF-2-alpha kinase GCN2 and related proteins; GCN2 (EC 2.7.11.1), also called eukaryotic translation initiation factor 2-alpha kinase 4 (EIF2AK4), acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability. It also plays a role in modulating the adaptive immune response to yellow fever virus infection and promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation.


Pssm-ID: 467659  Cd Length: 117  Bit Score: 52.99  E-value: 7.13e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  10 QRQNEEIEAMAAIYGEEWCVIDDCAKI-----FCIRISDDIDDPKW---TLCLQVMLPNEYPGTAPPIYQLNAPWLKGQE 81
Cdd:cd23823     2 EEQEEELEALQSIYGDDFEDLSSKKAVwsppeFRIRLRPQEGESEEnhvSVDLHVKFPPTYPDVPPEIELENVKGLSDEQ 81

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1519314609  82 RADLSNSLEEIYIQNIGESILYLWVEKIRDVL 113
Cdd:cd23823    82 LEELLKELEELAKELLGEEMIFELAEAVQEFL 113
RWD_RNF25 cd23818
RWD domain of RING finger protein 25 (RNF25) and related proteins; RNF25 (EC 2.3.2.27), also ...
 14-114 9.58e-09

RWD domain of RING finger protein 25 (RNF25) and related proteins; RNF25 (EC 2.3.2.27), also known as AO7, is a putative E3 ubiquitin-protein ligase that was initially identified as an interacting protein of the E2 ubiquitin-conjugating enzyme, Ubc5B. It is ubiquitously expressed in various tissues and is predominantly localized in the nucleus. RNF25 activates nuclear factor (NF)-kappaB-dependent gene expression upon stimulation with interleukin-1 beta (IL-1beta), or tumor necrosis factor (TNF), or overexpression of NF-kappaB-inducing kinase. It interacts with the p65 transactivation domain (TAD) and modulates its transcriptional activity.


Pssm-ID: 467654  Cd Length: 109  Bit Score: 52.54  E-value: 9.58e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  14 EEIEAMAAIYGEEWCVIDDCAKI----FCI--RISDDIDDPKWTLCLQVMLPNEYPGTAPPIYQLNAPWLKGQERADLSN 87
Cdd:cd23818     3 EELEALEAIYPDELKVVSEDGAPtelsITLhpATADDESEQYVRLTLVITLPPGYPEEPPKISLRNPRGLSDARLARLLS 82

                          90       100
                  ....*....|....*....|....*..
gi 1519314609  88 SLEEIYIQNIGESILYLWVEKIRDVLI 114
Cdd:cd23818    83 LLKELAEERAGEPMLFELIELAKEFLT 109
RWD_YLR419W-like cd23827
RWD domain of Saccharomyces cerevisiae putative ATP-dependent RNA helicase YLR419W and related ...
 13-70 2.80e-04

RWD domain of Saccharomyces cerevisiae putative ATP-dependent RNA helicase YLR419W and related proteins; YLR419W (EC 3.6.4.13) may act as an ATP-binding RNA helicase. The RWD domain may mediate protein-protein interactions.


Pssm-ID: 467662  Cd Length: 104  Bit Score: 39.54  E-value: 2.80e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1519314609  13 NEEIEAMAAIYGEEWCVIDDcaKIFCIRISDDIDDPKwTLCLQVMLPNEYPGTAPPIY 70
Cdd:cd23827     2 DEEIEALEAIYGEKFEVISD--DSCEITLNSPTKTKP-SLKLKFYKSSSYPNSLPGIF 56
RWD_RWDD1 cd23816
RWD domain of RWD domain-containing protein 1 (RWDD1) and related proteins; RWDD1, also called ...
 12-98 9.78e-04

RWD domain of RWD domain-containing protein 1 (RWDD1) and related proteins; RWDD1, also called DRG family-regulatory protein 2 (DFRP2), or PTD013, interacts with DRG2 and protects DRG2 from proteolytic degradation. It is an androgen receptor-interacting protein that functions as a coactivator of androgen-dependent transcription.


Pssm-ID: 467652  Cd Length: 118  Bit Score: 38.43  E-value: 9.78e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  12 QNEEIEAMAAIYGEEWCVIDDCAKI-FCIRI-SDDIDDPKWTL--CLQVMLPNEYPGTAPPIYQLNAPWLKGQERADLSN 87
Cdd:cd23816     5 QRNELEALESIYPDEFTVLSEEPPIsFTITVtSEEEENEDETVsvTLKFTYTEKYPDEAPLIEIISHENLEDEDIEDLLE 84

                          90
                  ....*....|.
gi 1519314609  88 SLEEIYIQNIG 98
Cdd:cd23816    85 LLEQQAEENLG 95
RWD_RWDD2 cd23829
RWD domain of RWD domain-containing protein 2A (RWDD2A), 2B (RWDD2B) and related proteins; ...
 12-111 3.18e-03

RWD domain of RWD domain-containing protein 2A (RWDD2A), 2B (RWDD2B) and related proteins; This subfamily includes RWDD2A, previously known as RWD domain-containing 2 (RWDD2) and RWDD2B, previously known in humans as chromosome 21 open reading frame (C21orf6). C21orf6 appears to be involved in monosomy 21 phenotype. The RWD domain may mediate protein-protein interactions.


Pssm-ID: 467663  Cd Length: 129  Bit Score: 37.17  E-value: 3.18e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  12 QNEEIEAMAAIY-GEEWCVIDD-------------------CAKI-FCIRISddIDDPKWTLCLQVMLPNEYPGTAPPIY 70
Cdd:cd23829     5 QLSEIEMLQSMFpNEGELKLDDpsavadlkrflegdtdsplPSRLeFTINLK--IEEPKVKVELSVTLPHEYPSVPPEIF 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 1519314609  71 QLNAPWLKGQERaDLSNSLEEiYIQNI--GESILYLWVEKIRD 111
Cdd:cd23829    83 VRSDSLSRSQQR-QLNEDLSE-YISSLerGELCILQIVQWLQD 123
RWD_ScYIH1-like cd23822
RWD domain of Saccharomyces cerevisiae protein YIH1 and related proteins; YIH1, also called ...
 13-113 4.32e-03

RWD domain of Saccharomyces cerevisiae protein YIH1 and related proteins; YIH1, also called protein IMPACT homolog, is an actin-binding protein that acts as a translational regulator which ensures constant high levels of translation under amino acid starvation. It plays a role as a negative regulator of GCN2 kinase activity. It impairs GCN1-mediated GCN2 activation, and hence GCN2-mediated eIF-2-alpha phosphorylation in amino acid-starved cells and subsequent down-regulation of protein synthesis. In normal conditions, it resides in an actin complex and has no activity.


Pssm-ID: 467658  Cd Length: 97  Bit Score: 36.08  E-value: 4.32e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519314609  13 NEEIEAMAAIYGEewCVIDDCAKIFCIRISDDIDdpkwtLCLQVMLPNEYPGTAPPIYQLNAPWLKGqERADLSNSLEEI 92
Cdd:cd23822     2 ADEIEAINAIYPD--CLVRLSPSIYTLKIPDHED-----VSIQLSFPSDYPDEPPHVLGVSSSGARG-DGKYLKDLLEDI 73

                          90       100
                  ....*....|....*....|...
gi 1519314609  93 YIQ--NIGESILYLWVEKIRDVL 113
Cdd:cd23822    74 LASvfVPGEVCLFDFIEELREVL 96
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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