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Conserved domains on  [gi|1519062650|gb|AYZ23503|]
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family 8 glycosyl transferase [Streptococcus agalactiae]

Protein Classification

glycosyltransferase family 8 protein( domain architecture ID 10135893)

glycosyltransferase family 8 protein similar to Neisseria meningitidis galactosyltransferase LgtC, which catalyzes a key step in the biosynthesis of lipooligosaccharide structure by transferring alpha-D-galactose from UDP-galactose to a terminal lactose

CAZY:  GT8
EC:  2.-.-.-
Gene Ontology:  GO:0016757
PubMed:  12691742|9445404
SCOP:  3000077

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
GT8_A4GalT_like cd04194
A4GalT_like proteins catalyze the addition of galactose or glucose residues to the ...
3-229 3.60e-73

A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface; The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.


:

Pssm-ID: 133037 [Multi-domain]  Cd Length: 248  Bit Score: 228.64  E-value: 3.60e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650   3 KAVALAVDSNYLDKALVTIKSICVYNR--NITFYLFNQDTPVEWVRNINRKLEPLGSKLINVKIYNYDIAHL---TTFLT 77
Cdd:cd04194     1 MNIVFAIDDNYAPYLAVTIKSILANNSkrDYDFYILNDDISEENKKKLKELLKKYNSSIEFIKIDNDDFKFFpatTDHIS 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  78 VSTWFRLFLADYIPS-SRVLYLDSDIIVNTNLDYLFELDFKGYYLAAVKDPHK-------------NEEGGFNAGMLLAN 143
Cdd:cd04194    81 YATYYRLLIPDLLPDyDKVLYLDADIIVLGDLSELFDIDLGDNLLAAVRDPFIeqekkrkrrlggyDDGSYFNSGVLLIN 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 144 LELWREDGLTKTLLKTAEELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQT-YDVVSRYNHRSYLYLNIENRTPNIIHFL 222
Cdd:cd04194   161 LKKWREENITEKLLELIKEYGGRLIYPDQDILNAVLKDKILYLPPRYNFQTgFYYLLKKKSKEEQELEEARKNPVIIHYT 240

                  ....*..
gi 1519062650 223 TSDKPWN 229
Cdd:cd04194   241 GSDKPWN 247
 
Name Accession Description Interval E-value
GT8_A4GalT_like cd04194
A4GalT_like proteins catalyze the addition of galactose or glucose residues to the ...
3-229 3.60e-73

A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface; The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.


Pssm-ID: 133037 [Multi-domain]  Cd Length: 248  Bit Score: 228.64  E-value: 3.60e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650   3 KAVALAVDSNYLDKALVTIKSICVYNR--NITFYLFNQDTPVEWVRNINRKLEPLGSKLINVKIYNYDIAHL---TTFLT 77
Cdd:cd04194     1 MNIVFAIDDNYAPYLAVTIKSILANNSkrDYDFYILNDDISEENKKKLKELLKKYNSSIEFIKIDNDDFKFFpatTDHIS 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  78 VSTWFRLFLADYIPS-SRVLYLDSDIIVNTNLDYLFELDFKGYYLAAVKDPHK-------------NEEGGFNAGMLLAN 143
Cdd:cd04194    81 YATYYRLLIPDLLPDyDKVLYLDADIIVLGDLSELFDIDLGDNLLAAVRDPFIeqekkrkrrlggyDDGSYFNSGVLLIN 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 144 LELWREDGLTKTLLKTAEELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQT-YDVVSRYNHRSYLYLNIENRTPNIIHFL 222
Cdd:cd04194   161 LKKWREENITEKLLELIKEYGGRLIYPDQDILNAVLKDKILYLPPRYNFQTgFYYLLKKKSKEEQELEEARKNPVIIHYT 240

                  ....*..
gi 1519062650 223 TSDKPWN 229
Cdd:cd04194   241 GSDKPWN 247
RfaJ COG1442
Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope ...
4-246 1.46e-64

Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441051 [Multi-domain]  Cd Length: 301  Bit Score: 208.29  E-value: 1.46e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650   4 AVALAVDSNYLDKALVTIKSICVYN--RNITFYLFNQDTPVEWVRNINRKLEPLGSKL--INVKIYNYDIAHLTTFLTVS 79
Cdd:COG1442     7 NIVFAIDDNYLPGLGVSIASLLENNpdRPYDFHILTDGLSDENKERLEALAAKYNVSIefIDVDDELLKDLPVSKHISKA 86
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  80 TWFRLFLADYIPSS--RVLYLDSDIIVNTNLDYLFELDFKGYYLAAVKDPH--------------KNEEGGFNAGMLLAN 143
Cdd:COG1442    87 TYYRLLIPELLPDDydKVLYLDADTLVLGDLSELWDIDLGGNLLAAVRDGTvtgsqkkrakrlglPDDDGYFNSGVLLIN 166
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 144 LELWREDGLTKTLLKTAEELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSRYNHRSYLYLNIENRTPNIIHFLT 223
Cdd:COG1442   167 LKKWREENITEKALEFLKENPDKLKYPDQDILNIVLGGKVKFLPPRYNYQYSLYYELKDKSNKKELLEARKNPVIIHYTG 246
                         250       260
                  ....*....|....*....|...
gi 1519062650 224 SDKPWNENSVARFRELWWYYFQL 246
Cdd:COG1442   247 PTKPWHKWCTHPYADLYWEYLKK 269
Glyco_transf_8 pfam01501
Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to ...
5-231 7.63e-48

Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.


Pssm-ID: 279798 [Multi-domain]  Cd Length: 252  Bit Score: 163.26  E-value: 7.63e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650   5 VALAVDSNYLDKALVTIKSICVYN--RNITFYLFNQDTPVEWVRNINRKLEPLGSKLI-----NVKIYNYDIAHLTT--F 75
Cdd:pfam01501   2 IALALDKNYLLGASVSIKSLLKNNsdFALNFHIFTDDIPVENLDILNWLASSYKPVLPllesdIKIFEYFSKLKLRSpkY 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  76 LTVSTWFRLFLADYIPS-SRVLYLDSDIIVNTNLDYLFELDFKGYYLAAVKDPHKN----------------EEGGFNAG 138
Cdd:pfam01501  82 WSLLNYLRLYLPDLFPKlDKILYLDADIVVQGDLSPLWDIDLGGKVLAAVEDNYFQrypnfsepiilenfgpPACYFNAG 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 139 MLLANLELWREDGLTKTLLKT--AEELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDvvsRYNHRSylYLNIENRTP 216
Cdd:pfam01501 162 MLLFDLDAWRKENITERYIKWlnLNENRTLWKLGDQDPLNIVFYGKVKPLDPRWNVLGLG---YYNKKK--SLNEITENA 236
                         250
                  ....*....|....*
gi 1519062650 217 NIIHFLTSDKPWNEN 231
Cdd:pfam01501 237 AVIHYNGPTKPWLDI 251
PRK15171 PRK15171
lipopolysaccharide 3-alpha-galactosyltransferase;
5-230 1.62e-13

lipopolysaccharide 3-alpha-galactosyltransferase;


Pssm-ID: 185093 [Multi-domain]  Cd Length: 334  Bit Score: 70.94  E-value: 1.62e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650   5 VALAVDSNYLDKALVTIKSICVYNRNIT--FYLFNQDTPVEWVRNINRKLEPLGSKlinVKIYNYDIAHL-----TTFLT 77
Cdd:PRK15171   28 IAYGIDKNFLFGCGVSIASVLLNNPDKSlvFHVFTDYISDADKQRFSALAKQYNTR---INIYLINCERLkslpsTKNWT 104
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  78 VSTWFRLFLADYI--PSSRVLYLDSDIIVNTNLDYLFELDFKGYYLAAV---KDPH--KN----------EEGGFNAGML 140
Cdd:PRK15171  105 YATYFRFIIADYFidKTDKVLYLDADIACKGSIKELIDLDFAENEIAAVvaeGDAEwwSKraqslqtpglASGYFNSGFL 184
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 141 LANLELWREDGLTK---TLLKTAEELHRVVKTgDQSILNIVCHNrwlslnktwnfQTYDVVSRYNHRSYLYLNIENRTPN 217
Cdd:PRK15171  185 LINIPAWAQENISAkaiEMLADPEIVSRITHL-DQDVLNILLAG-----------KVKFIDAKYNTQFSLNYELKDSVIN 252
                         250       260
                  ....*....|....*....|
gi 1519062650 218 -------IIHFLTSDKPWNE 230
Cdd:PRK15171  253 pvndetvFIHYIGPTKPWHS 272
 
Name Accession Description Interval E-value
GT8_A4GalT_like cd04194
A4GalT_like proteins catalyze the addition of galactose or glucose residues to the ...
3-229 3.60e-73

A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface; The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.


Pssm-ID: 133037 [Multi-domain]  Cd Length: 248  Bit Score: 228.64  E-value: 3.60e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650   3 KAVALAVDSNYLDKALVTIKSICVYNR--NITFYLFNQDTPVEWVRNINRKLEPLGSKLINVKIYNYDIAHL---TTFLT 77
Cdd:cd04194     1 MNIVFAIDDNYAPYLAVTIKSILANNSkrDYDFYILNDDISEENKKKLKELLKKYNSSIEFIKIDNDDFKFFpatTDHIS 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  78 VSTWFRLFLADYIPS-SRVLYLDSDIIVNTNLDYLFELDFKGYYLAAVKDPHK-------------NEEGGFNAGMLLAN 143
Cdd:cd04194    81 YATYYRLLIPDLLPDyDKVLYLDADIIVLGDLSELFDIDLGDNLLAAVRDPFIeqekkrkrrlggyDDGSYFNSGVLLIN 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 144 LELWREDGLTKTLLKTAEELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQT-YDVVSRYNHRSYLYLNIENRTPNIIHFL 222
Cdd:cd04194   161 LKKWREENITEKLLELIKEYGGRLIYPDQDILNAVLKDKILYLPPRYNFQTgFYYLLKKKSKEEQELEEARKNPVIIHYT 240

                  ....*..
gi 1519062650 223 TSDKPWN 229
Cdd:cd04194   241 GSDKPWN 247
RfaJ COG1442
Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope ...
4-246 1.46e-64

Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441051 [Multi-domain]  Cd Length: 301  Bit Score: 208.29  E-value: 1.46e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650   4 AVALAVDSNYLDKALVTIKSICVYN--RNITFYLFNQDTPVEWVRNINRKLEPLGSKL--INVKIYNYDIAHLTTFLTVS 79
Cdd:COG1442     7 NIVFAIDDNYLPGLGVSIASLLENNpdRPYDFHILTDGLSDENKERLEALAAKYNVSIefIDVDDELLKDLPVSKHISKA 86
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  80 TWFRLFLADYIPSS--RVLYLDSDIIVNTNLDYLFELDFKGYYLAAVKDPH--------------KNEEGGFNAGMLLAN 143
Cdd:COG1442    87 TYYRLLIPELLPDDydKVLYLDADTLVLGDLSELWDIDLGGNLLAAVRDGTvtgsqkkrakrlglPDDDGYFNSGVLLIN 166
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 144 LELWREDGLTKTLLKTAEELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSRYNHRSYLYLNIENRTPNIIHFLT 223
Cdd:COG1442   167 LKKWREENITEKALEFLKENPDKLKYPDQDILNIVLGGKVKFLPPRYNYQYSLYYELKDKSNKKELLEARKNPVIIHYTG 246
                         250       260
                  ....*....|....*....|...
gi 1519062650 224 SDKPWNENSVARFRELWWYYFQL 246
Cdd:COG1442   247 PTKPWHKWCTHPYADLYWEYLKK 269
Glyco_transf_8 pfam01501
Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to ...
5-231 7.63e-48

Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.


Pssm-ID: 279798 [Multi-domain]  Cd Length: 252  Bit Score: 163.26  E-value: 7.63e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650   5 VALAVDSNYLDKALVTIKSICVYN--RNITFYLFNQDTPVEWVRNINRKLEPLGSKLI-----NVKIYNYDIAHLTT--F 75
Cdd:pfam01501   2 IALALDKNYLLGASVSIKSLLKNNsdFALNFHIFTDDIPVENLDILNWLASSYKPVLPllesdIKIFEYFSKLKLRSpkY 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  76 LTVSTWFRLFLADYIPS-SRVLYLDSDIIVNTNLDYLFELDFKGYYLAAVKDPHKN----------------EEGGFNAG 138
Cdd:pfam01501  82 WSLLNYLRLYLPDLFPKlDKILYLDADIVVQGDLSPLWDIDLGGKVLAAVEDNYFQrypnfsepiilenfgpPACYFNAG 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 139 MLLANLELWREDGLTKTLLKT--AEELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDvvsRYNHRSylYLNIENRTP 216
Cdd:pfam01501 162 MLLFDLDAWRKENITERYIKWlnLNENRTLWKLGDQDPLNIVFYGKVKPLDPRWNVLGLG---YYNKKK--SLNEITENA 236
                         250
                  ....*....|....*
gi 1519062650 217 NIIHFLTSDKPWNEN 231
Cdd:pfam01501 237 AVIHYNGPTKPWLDI 251
GT8_Glycogenin cd02537
Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen; Glycogenin ...
5-245 5.49e-18

Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen; Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases.


Pssm-ID: 133018 [Multi-domain]  Cd Length: 240  Bit Score: 82.69  E-value: 5.49e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650   5 VALAVDSNYLDKALVTIKSICVYNRNITFYLFNQDTPVEWVRNInrkLEPLGSKLINV-KIYNYDIAHL---TTFLTVST 80
Cdd:cd02537     4 VTLLTNDDYLPGALVLGYSLRKVGSSYDLVVLVTPGVSEESREA---LEEVGWIVREVePIDPPDSANLlkrPRFKDTYT 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  81 WFRLF-LADYipsSRVLYLDSDIIVNTNLDYLFELDFkgyYLAAVKD---PHKneeggFNAGMLLA--NLELWREdgltk 154
Cdd:cd02537    81 KLRLWnLTEY---DKVVFLDADTLVLRNIDELFDLPG---EFAAAPDcgwPDL-----FNSGVFVLkpSEETFND----- 144
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 155 tLLktaEELHRVVKT--GDQSILNIVCHNR--WLSLNKTWNfqtYDVVSRYNHRSYLYLNIENRtpnIIHFLTSDKPWNE 230
Cdd:cd02537   145 -LL---DALQDTPSFdgGDQGLLNSYFSDRgiWKRLPFTYN---ALKPLRYLHPEALWFGDEIK---VVHFIGGDKPWSW 214
                         250       260
                  ....*....|....*....|....
gi 1519062650 231 NS---------VARFRELWWYYFQ 245
Cdd:cd02537   215 WRdpetkekddYNELHQWWWDIYD 238
Glyco_transf_8 cd00505
Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis ...
5-229 6.44e-17

Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis; Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.


Pssm-ID: 132996 [Multi-domain]  Cd Length: 246  Bit Score: 79.79  E-value: 6.44e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650   5 VALAVDSNYLDKALVTIKSICVYNRN-ITFYLFnqDTPVEWVRN----INRKLEPLGSKLINVKIY-NYDIAHLTTFLTV 78
Cdd:cd00505     4 VIVATGDEYLRGAIVLMKSVLRHRTKpLRFHVL--TNPLSDTFKaaldNLRKLYNFNYELIPVDILdSVDSEHLKRPIKI 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  79 STWFRLFLADYIPS-SRVLYLDSDIIVNTNLDYLFELDFKGYYLAAVKD--------------PHKNEEGGFNAGMLLAN 143
Cdd:cd00505    82 VTLTKLHLPNLVPDyDKILYVDADILVLTDIDELWDTPLGGQELAAAPDpgdrregkyyrqkrSHLAGPDYFNSGVFVVN 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 144 LELWREDGLTKTLLKTAEELHRVVKTGDQSILNIVCHNRWL---SLNKTWNFQTYDVVSRYNHRSYLYLNIEnrtpnIIH 220
Cdd:cd00505   162 LSKERRNQLLKVALEKWLQSLSSLSGGDQDLLNTFFKQVPFivkSLPCIWNVRLTGCYRSLNCFKAFVKNAK-----VIH 236

                  ....*....
gi 1519062650 221 FLTSDKPWN 229
Cdd:cd00505   237 FNGPTKPWN 245
GT8_like_1 cd06429
GT8_like_1 represents a subfamily of GT8 with unknown function; A subfamily of ...
83-244 1.41e-14

GT8_like_1 represents a subfamily of GT8 with unknown function; A subfamily of glycosyltransferase family 8 with unknown function: Glycosyltransferase family 8 comprises enzymes with a number of known activities; lipopolysaccharide galactosyltransferase lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase and inositol 1-alpha-galactosyltransferase. It is classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed.


Pssm-ID: 133051 [Multi-domain]  Cd Length: 257  Bit Score: 73.19  E-value: 1.41e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  83 RLFLADYIPS-SRVLYLDSDIIVNTNLDYLFELDFKGYYLAAVkdphkneEGGFNAGMLLANLELWREDGLTKTLLK--- 158
Cdd:cd06429   104 RFYLPELFPKlEKVIYLDDDVVVQKDLTELWNTDLGGGVAGAV-------ETSWNPGVNVVNLTEWRRQNVTETYEKwme 176
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 159 -TAEELHRVVKTGDQSILNIVCHNRWLSLNKTWNFqtydvvsRYNHRSYLYLNIENRTPNIIHFLTSDKPWNENSVARFR 237
Cdd:cd06429   177 lNQEEEVTLWKLITLPPGLIVFYGLTSPLDPSWHV-------RGLGYNYGIRPQDIKAAAVLHFNGNMKPWLRTAIPSYK 249

                  ....*..
gi 1519062650 238 ELWWYYF 244
Cdd:cd06429   250 ELWEKYL 256
PRK15171 PRK15171
lipopolysaccharide 3-alpha-galactosyltransferase;
5-230 1.62e-13

lipopolysaccharide 3-alpha-galactosyltransferase;


Pssm-ID: 185093 [Multi-domain]  Cd Length: 334  Bit Score: 70.94  E-value: 1.62e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650   5 VALAVDSNYLDKALVTIKSICVYNRNIT--FYLFNQDTPVEWVRNINRKLEPLGSKlinVKIYNYDIAHL-----TTFLT 77
Cdd:PRK15171   28 IAYGIDKNFLFGCGVSIASVLLNNPDKSlvFHVFTDYISDADKQRFSALAKQYNTR---INIYLINCERLkslpsTKNWT 104
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  78 VSTWFRLFLADYI--PSSRVLYLDSDIIVNTNLDYLFELDFKGYYLAAV---KDPH--KN----------EEGGFNAGML 140
Cdd:PRK15171  105 YATYFRFIIADYFidKTDKVLYLDADIACKGSIKELIDLDFAENEIAAVvaeGDAEwwSKraqslqtpglASGYFNSGFL 184
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 141 LANLELWREDGLTK---TLLKTAEELHRVVKTgDQSILNIVCHNrwlslnktwnfQTYDVVSRYNHRSYLYLNIENRTPN 217
Cdd:PRK15171  185 LINIPAWAQENISAkaiEMLADPEIVSRITHL-DQDVLNILLAG-----------KVKFIDAKYNTQFSLNYELKDSVIN 252
                         250       260
                  ....*....|....*....|
gi 1519062650 218 -------IIHFLTSDKPWNE 230
Cdd:PRK15171  253 pvndetvFIHYIGPTKPWHS 272
GT8_LARGE_C cd06431
LARGE catalytic domain has closest homology to GT8 glycosyltransferase involved in ...
83-247 2.07e-05

LARGE catalytic domain has closest homology to GT8 glycosyltransferase involved in lipooligosaccharide synthesis; The catalytic domain of LARGE is a putative glycosyltransferase. Mutations of LARGE in mouse and human cause dystroglycanopathies, a disease associated with hypoglycosylation of the membrane protein alpha-dystroglycan (alpha-DG) and consequent loss of extracellular ligand binding. LARGE needs to both physically interact with alpha-dystroglycan and function as a glycosyltransferase in order to stimulate alpha-dystroglycan hyperglycosylation. LARGE localizes to the Golgi apparatus and contains three conserved DxD motifs. While two of the motifs are indispensible for glycosylation function, one is important for localization of th eenzyme. LARGE was originally named because it covers approximately large trunck of genomic DNA, more than 600bp long. The predicted protein structure contains an N-terminal cytoplasmic domain, a transmembrane region, a coiled-coil motif, and two putative catalytic domains. This catalytic domain has closest homology to GT8 glycosyltransferase involved in lipooligosaccharide synthesis.


Pssm-ID: 133053  Cd Length: 280  Bit Score: 45.93  E-value: 2.07e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  83 RLFLADYIPS--SRVLYLDSDIIVNTNLDYLFEL--DFKG------------YYLAAVKDPHKNEEG---GFNAGMLLAN 143
Cdd:cd06431    87 KLVLTEALPSdlEKVIVLDTDITFATDIAELWKIfhKFTGqqvlglvenqsdWYLGNLWKNHRPWPAlgrGFNTGVILLD 166
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 144 LELWREDGLTKTLLKTAE-ELHRVVKT--GDQSILNIVCHNRWLSLNK---TWNFQTYDvvsryNHRS-YLYLNIEnrTP 216
Cdd:cd06431   167 LDKLRKMKWESMWRLTAErELMSMLSTslADQDIFNAVIKQNPFLVYQlpcAWNVQLSD-----HTRSeQCYRDVS--DL 239
                         170       180       190
                  ....*....|....*....|....*....|..
gi 1519062650 217 NIIHFLTSDKPWNENS-VARFRELWWYYFQLD 247
Cdd:cd06431   240 KVIHWNSPKKLRVKNKhVEFFRNLYLTFLEYD 271
GT8_like_2 cd06430
GT8_like_2 represents a subfamily of GT8 with unknown function; A subfamily of ...
14-180 3.19e-05

GT8_like_2 represents a subfamily of GT8 with unknown function; A subfamily of glycosyltransferase family 8 with unknown function: Glycosyltransferase family 8 comprises enzymes with a number of known activities; lipopolysaccharide galactosyltransferase lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase and inositol 1-alpha-galactosyltransferase. It is classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed.


Pssm-ID: 133052  Cd Length: 304  Bit Score: 45.53  E-value: 3.19e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  14 LDKALVTIKSICVYNRN-ITFYLFNQDtpvEWVRNINRKLEPLgsKLINVKIYNYDIaHLTTFLTVST--W---F----- 82
Cdd:cd06430    12 LEETLTMLKSAIVFSQKpLRFHIFAED---QLKQSFKEKLDDW--PELIDRKFNYTL-HPITFPSGNAaeWkklFkpcaa 85
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  83 -RLFLADYIPS-SRVLYLDSDIIVNTNLDYLFEL--DFKGYYLAAVKDPHKNE---------------EGGFNAGMLLAN 143
Cdd:cd06430    86 qRLFLPSLLPDvDSLLYVDTDILFLRPVEEIWSFlkKFNSTQLAAMAPEHEEPnigwynrfarhpyygKTGVNSGVMLMN 165
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 1519062650 144 LELWRE----DGLTKTLLKTAEELHRVVKT-------GDQSILNIVCH 180
Cdd:cd06430   166 LTRMRRkyfkNDMTPVGLRWEEILMPLYKKyklkitwGDQDLINIIFH 213
PLN02829 PLN02829
Probable galacturonosyltransferase
75-247 7.89e-05

Probable galacturonosyltransferase


Pssm-ID: 215443 [Multi-domain]  Cd Length: 639  Bit Score: 44.84  E-value: 7.89e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650  75 FLTVSTWFRLFLADYIPS-SRVLYLDSDIIVNTNLDYLFELDFKGYYLAAVK------------------------DPHK 129
Cdd:PLN02829  439 YLSILNHLRFYLPEIFPKlNKVLFLDDDIVVQKDLTGLWSIDLKGNVNGAVEtcgesfhrfdrylnfsnplisknfDPHA 518
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519062650 130 neeGGFNAGMLLANLELWREDGLTKtLLKTAEELHR---VVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSRYNHRsy 206
Cdd:PLN02829  519 ---CGWAYGMNVFDLDEWKRQNITE-VYHSWQKLNHdrqLWKLGTLPPGLITFWKRTYPLDRSWHVLGLGYNPNVNQR-- 592
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 1519062650 207 lylNIENRTpnIIHFLTSDKPWNENSVARFRELWWYYFQLD 247
Cdd:PLN02829  593 ---DIERAA--VIHYNGNMKPWLEIGIPKYRNYWSKYVDYD 628
PLN00176 PLN00176
galactinol synthase
93-141 1.17e-03

galactinol synthase


Pssm-ID: 215090 [Multi-domain]  Cd Length: 333  Bit Score: 40.45  E-value: 1.17e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1519062650  93 SRVLYLDSDIIVNTNLDYLFELDfKGyYLAAVKD---------------------PHK---NEEGG------FNAGMLL 141
Cdd:PLN00176  114 SKMIYLDGDIQVFENIDHLFDLP-DG-YFYAVMDcfcektwshtpqykigycqqcPDKvtwPAELGpppplyFNAGMFV 190
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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