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Conserved domains on  [gi|151101235|ref|NP_036593|]
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TERF1-interacting nuclear factor 2 isoform 2 [Homo sapiens]

Protein Classification

TIN2_N and TIN2_TBM domain-containing protein( domain architecture ID 11237163)

TIN2_N and TIN2_TBM domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TINF2_N pfam14973
TERF1-interacting nuclear factor 2 N-terminus; This is the N-terminus of TERF1-interacting ...
 20-169 4.44e-75

TERF1-interacting nuclear factor 2 N-terminus; This is the N-terminus of TERF1-interacting nuclear factor 2. It is required for the formation of the shelterin complex. The shelterin complex is involved in the protection and maintenance of telomeres.


:

Pssm-ID: 464415  Cd Length: 148  Bit Score: 228.32  E-value: 4.44e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 151101235   20 WQVVRGRCVEHFPRVLEFLRSLRAVAPGLVRYRHHERLCMGLKAKVVVELILQGRPwaQVLKALNHHFPESGPIVrDPKA 99
Cdd:pfam14973   1 WQVVQQRDVEHYGKVEEFVSLVTEAVPGLLSYRHHAQLIMGLRAKLILELCRGERP--QDLKAIQSHLDRLGPTV-SPKA 77

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 151101235  100 TKQDLRKILEAQETFYQQVKQLSEAPVDLASKLQE-LEQEYGEPFLAAMEKLLFEYLCQLEKALPTPQAQQ 169
Cdd:pfam14973  78 TKQDDVKVEEAQENFLQLVQSLLEDPVEREHFFQEvFPVEYGPKFDAALEKLVWEFLSRLEQLLPVPDLQQ 148
TIN2_TBM cd11741
TRF-binding motif region of TRF-Interacting Nuclear factor 2; The C-terminal region of TIN2 ...
 249-353 5.76e-36

TRF-binding motif region of TRF-Interacting Nuclear factor 2; The C-terminal region of TIN2 contains the TRF-binding motif (TBM), while the TIN2 N-terminal region acts in the modulation of TRF1 activity via the inhibition of tankyrase 1. TIN2 binding to TRF2 is primarily via the TRF binding motif (TBM) and the N-terminus, while the far C-terminal region interacts with lower affinity. The TIN2 TBM, but not the N-terminal region, is involved in TIN2 binding to TRF1. Truncation of the TIN2 N-terminus in mouse results in telomere elongation, suggesting a a negative regulatory function of this region. TIN2 is a shelterin complex protein identified in mammals, one of 6 factors that act to protect telomeres from DNA damage repair machinery. Three shelterin components (TRF1, TRF2, POT1) bind DNA and 3 components (TIN2, RAP1, TPP1) are recruited by these DNA binding factors. TIN2 binds directly to TRF1 and TRF2 and stabilizes TRF2 complex-telomere binding by tethering it to the TRF1 complex. TRF1 activity at telomeres is regulated in part by selective ubiquitination and degradation. Ubiquitination of TRF1 is mediated by Fbx4, which binds TRF1 in the TRFH domain, via a small GTPase module. When bound to telomeres, TIN2 acts to protect TRF1 from SCF-Fbx4 mediated ubiquitination. F-box proteins act in substrate recognition as part of SCF complexes (SCF: Skp1-Cul1-Rbx1-F- box protein). Tankyrase-mediated ADP-ribosylation releases TRF1 from telomeres, rendering them susceptible to ubiquitination and degradation, promoting telomere elongation. TIN2 also binds TPP1, which recruits POT1 to telomeres.


:

Pssm-ID: 240666 [Multi-domain]  Cd Length: 108  Bit Score: 126.48  E-value: 5.76e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 151101235 249 HPEPLAGRHFNLAPLGRRRVQSQWASTRGGHKERPTVMLFPFRNLGSPTQVISK-PESKEEHAIYTADLAMGTRAASTGK 327
Cdd:cd11741    1 PPSSLTGRGFGLAPLSKRKSQSRWPSAPRSHKERPTTMLPPRRSTKLPTQKPSRsPASREEHGNNDAAASMGTRSSSTPK 80

                         90       100
                 ....*....|....*....|....*...
gi 151101235 328 SKSP--CQTLGGRALKENPVDLPATEQK 353
Cdd:cd11741   81 AKSPdpQDPLGRRSQERPPCSSAASNSF 108
 
Name Accession Description Interval E-value
TINF2_N pfam14973
TERF1-interacting nuclear factor 2 N-terminus; This is the N-terminus of TERF1-interacting ...
 20-169 4.44e-75

TERF1-interacting nuclear factor 2 N-terminus; This is the N-terminus of TERF1-interacting nuclear factor 2. It is required for the formation of the shelterin complex. The shelterin complex is involved in the protection and maintenance of telomeres.


Pssm-ID: 464415  Cd Length: 148  Bit Score: 228.32  E-value: 4.44e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 151101235   20 WQVVRGRCVEHFPRVLEFLRSLRAVAPGLVRYRHHERLCMGLKAKVVVELILQGRPwaQVLKALNHHFPESGPIVrDPKA 99
Cdd:pfam14973   1 WQVVQQRDVEHYGKVEEFVSLVTEAVPGLLSYRHHAQLIMGLRAKLILELCRGERP--QDLKAIQSHLDRLGPTV-SPKA 77

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 151101235  100 TKQDLRKILEAQETFYQQVKQLSEAPVDLASKLQE-LEQEYGEPFLAAMEKLLFEYLCQLEKALPTPQAQQ 169
Cdd:pfam14973  78 TKQDDVKVEEAQENFLQLVQSLLEDPVEREHFFQEvFPVEYGPKFDAALEKLVWEFLSRLEQLLPVPDLQQ 148
TIN2_N cd11657
N-terminal domain of TRF-interacting nuclear factor 2; shelterin complex protein of telomeres; ...
 20-201 2.47e-55

N-terminal domain of TRF-interacting nuclear factor 2; shelterin complex protein of telomeres; TIN2 is one of the six proteins of shelterin complex, which acts to protect telomeres from DNA damage repair machinery. TIN2 binds directly to TRF1 and TRF2 and stabilizes TRF2 complex-telomere binding by tethering it to the TRF1 complex. TIN2 binding to TRF2 is primarily via the TRF binding motif (TBM) region and the N-terminus, while the far C-terminal region has lower affinity. The TIN2 TBM, but not the N-terminal region, is involved in TIN2 binding to TRF1. Truncation of the TIN2 N-terminus in mouse results in telomere elongation, suggesting a negative regulatory function of this region. Three shelterin components (TRF1, TRF2, POT1) bind DNA and 3 components (TIN2, RAP1, TPP1) are recruited by these DNA binding factors. TRF1 activity at telomeres is regulated in part by selective ubiquitination and degradation. Ubiquitination of TRF1 is mediated by Fbx4, which binds TRF1 in the TRFH domain, via a small GTPase module. When bound to telomeres, TIN2 acts to protect TRF1 from SCF-Fbx4 mediated ubiquitination. F-box proteins act in substrate recognition as part of Skp1-Cul1-Rbx1-F- box (SCF) protein complexes. Tankyrase-mediated ADP-ribosylation releases TRF1 from telomeres, rendering them susceptible to ubiquitination and degradation, promoting telomere elongation. TIN2 also binds PIP1, which recruits POT1 to telomeres.


Pssm-ID: 240667  Cd Length: 188  Bit Score: 179.41  E-value: 2.47e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 151101235  20 WQVVRGRCVEHFPRVLEFLRSLRAVAPGLVRYRHHERLCMGLKAKVVVELILqgRPWAQVLKALNHHFPESGPIVRDPKa 99
Cdd:cd11657   12 WQVVQRRDVKHYGKVEEFVSLVTETVPELLTFRQRAKLILGLRARVILELCR--NEKAADLAALNPHLPRLLPPYPNKC- 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 151101235 100 tKQDLRKILEAQETFYQQV-KQLSEAPVDLASKLQELEQEYGEPFLAAMEKLLFEYLCQLEKALPTPQAQQLQDVLSWMQ 178
Cdd:cd11657   89 -QREDALMEEPQLNFLQLVqSLLKDPEEREHFFQEVFPVEYGEEFDQALEKLLWEFLSRLEKLLPVPDLKQTVSWLSTSP 167

                        170       180
                 ....*....|....*....|...
gi 151101235 179 PGVSITssLAWRQYGVDMGWLLP 201
Cdd:cd11657  168 SVLEEC--LDSVSDPDDLKSLLQ 188
TIN2_TBM cd11741
TRF-binding motif region of TRF-Interacting Nuclear factor 2; The C-terminal region of TIN2 ...
 249-353 5.76e-36

TRF-binding motif region of TRF-Interacting Nuclear factor 2; The C-terminal region of TIN2 contains the TRF-binding motif (TBM), while the TIN2 N-terminal region acts in the modulation of TRF1 activity via the inhibition of tankyrase 1. TIN2 binding to TRF2 is primarily via the TRF binding motif (TBM) and the N-terminus, while the far C-terminal region interacts with lower affinity. The TIN2 TBM, but not the N-terminal region, is involved in TIN2 binding to TRF1. Truncation of the TIN2 N-terminus in mouse results in telomere elongation, suggesting a a negative regulatory function of this region. TIN2 is a shelterin complex protein identified in mammals, one of 6 factors that act to protect telomeres from DNA damage repair machinery. Three shelterin components (TRF1, TRF2, POT1) bind DNA and 3 components (TIN2, RAP1, TPP1) are recruited by these DNA binding factors. TIN2 binds directly to TRF1 and TRF2 and stabilizes TRF2 complex-telomere binding by tethering it to the TRF1 complex. TRF1 activity at telomeres is regulated in part by selective ubiquitination and degradation. Ubiquitination of TRF1 is mediated by Fbx4, which binds TRF1 in the TRFH domain, via a small GTPase module. When bound to telomeres, TIN2 acts to protect TRF1 from SCF-Fbx4 mediated ubiquitination. F-box proteins act in substrate recognition as part of SCF complexes (SCF: Skp1-Cul1-Rbx1-F- box protein). Tankyrase-mediated ADP-ribosylation releases TRF1 from telomeres, rendering them susceptible to ubiquitination and degradation, promoting telomere elongation. TIN2 also binds TPP1, which recruits POT1 to telomeres.


Pssm-ID: 240666 [Multi-domain]  Cd Length: 108  Bit Score: 126.48  E-value: 5.76e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 151101235 249 HPEPLAGRHFNLAPLGRRRVQSQWASTRGGHKERPTVMLFPFRNLGSPTQVISK-PESKEEHAIYTADLAMGTRAASTGK 327
Cdd:cd11741    1 PPSSLTGRGFGLAPLSKRKSQSRWPSAPRSHKERPTTMLPPRRSTKLPTQKPSRsPASREEHGNNDAAASMGTRSSSTPK 80

                         90       100
                 ....*....|....*....|....*...
gi 151101235 328 SKSP--CQTLGGRALKENPVDLPATEQK 353
Cdd:cd11741   81 AKSPdpQDPLGRRSQERPPCSSAASNSF 108
 
Name Accession Description Interval E-value
TINF2_N pfam14973
TERF1-interacting nuclear factor 2 N-terminus; This is the N-terminus of TERF1-interacting ...
 20-169 4.44e-75

TERF1-interacting nuclear factor 2 N-terminus; This is the N-terminus of TERF1-interacting nuclear factor 2. It is required for the formation of the shelterin complex. The shelterin complex is involved in the protection and maintenance of telomeres.


Pssm-ID: 464415  Cd Length: 148  Bit Score: 228.32  E-value: 4.44e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 151101235   20 WQVVRGRCVEHFPRVLEFLRSLRAVAPGLVRYRHHERLCMGLKAKVVVELILQGRPwaQVLKALNHHFPESGPIVrDPKA 99
Cdd:pfam14973   1 WQVVQQRDVEHYGKVEEFVSLVTEAVPGLLSYRHHAQLIMGLRAKLILELCRGERP--QDLKAIQSHLDRLGPTV-SPKA 77

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 151101235  100 TKQDLRKILEAQETFYQQVKQLSEAPVDLASKLQE-LEQEYGEPFLAAMEKLLFEYLCQLEKALPTPQAQQ 169
Cdd:pfam14973  78 TKQDDVKVEEAQENFLQLVQSLLEDPVEREHFFQEvFPVEYGPKFDAALEKLVWEFLSRLEQLLPVPDLQQ 148
TIN2_N cd11657
N-terminal domain of TRF-interacting nuclear factor 2; shelterin complex protein of telomeres; ...
 20-201 2.47e-55

N-terminal domain of TRF-interacting nuclear factor 2; shelterin complex protein of telomeres; TIN2 is one of the six proteins of shelterin complex, which acts to protect telomeres from DNA damage repair machinery. TIN2 binds directly to TRF1 and TRF2 and stabilizes TRF2 complex-telomere binding by tethering it to the TRF1 complex. TIN2 binding to TRF2 is primarily via the TRF binding motif (TBM) region and the N-terminus, while the far C-terminal region has lower affinity. The TIN2 TBM, but not the N-terminal region, is involved in TIN2 binding to TRF1. Truncation of the TIN2 N-terminus in mouse results in telomere elongation, suggesting a negative regulatory function of this region. Three shelterin components (TRF1, TRF2, POT1) bind DNA and 3 components (TIN2, RAP1, TPP1) are recruited by these DNA binding factors. TRF1 activity at telomeres is regulated in part by selective ubiquitination and degradation. Ubiquitination of TRF1 is mediated by Fbx4, which binds TRF1 in the TRFH domain, via a small GTPase module. When bound to telomeres, TIN2 acts to protect TRF1 from SCF-Fbx4 mediated ubiquitination. F-box proteins act in substrate recognition as part of Skp1-Cul1-Rbx1-F- box (SCF) protein complexes. Tankyrase-mediated ADP-ribosylation releases TRF1 from telomeres, rendering them susceptible to ubiquitination and degradation, promoting telomere elongation. TIN2 also binds PIP1, which recruits POT1 to telomeres.


Pssm-ID: 240667  Cd Length: 188  Bit Score: 179.41  E-value: 2.47e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 151101235  20 WQVVRGRCVEHFPRVLEFLRSLRAVAPGLVRYRHHERLCMGLKAKVVVELILqgRPWAQVLKALNHHFPESGPIVRDPKa 99
Cdd:cd11657   12 WQVVQRRDVKHYGKVEEFVSLVTETVPELLTFRQRAKLILGLRARVILELCR--NEKAADLAALNPHLPRLLPPYPNKC- 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 151101235 100 tKQDLRKILEAQETFYQQV-KQLSEAPVDLASKLQELEQEYGEPFLAAMEKLLFEYLCQLEKALPTPQAQQLQDVLSWMQ 178
Cdd:cd11657   89 -QREDALMEEPQLNFLQLVqSLLKDPEEREHFFQEVFPVEYGEEFDQALEKLLWEFLSRLEKLLPVPDLKQTVSWLSTSP 167

                        170       180
                 ....*....|....*....|...
gi 151101235 179 PGVSITssLAWRQYGVDMGWLLP 201
Cdd:cd11657  168 SVLEEC--LDSVSDPDDLKSLLQ 188
TIN2_TBM cd11741
TRF-binding motif region of TRF-Interacting Nuclear factor 2; The C-terminal region of TIN2 ...
 249-353 5.76e-36

TRF-binding motif region of TRF-Interacting Nuclear factor 2; The C-terminal region of TIN2 contains the TRF-binding motif (TBM), while the TIN2 N-terminal region acts in the modulation of TRF1 activity via the inhibition of tankyrase 1. TIN2 binding to TRF2 is primarily via the TRF binding motif (TBM) and the N-terminus, while the far C-terminal region interacts with lower affinity. The TIN2 TBM, but not the N-terminal region, is involved in TIN2 binding to TRF1. Truncation of the TIN2 N-terminus in mouse results in telomere elongation, suggesting a a negative regulatory function of this region. TIN2 is a shelterin complex protein identified in mammals, one of 6 factors that act to protect telomeres from DNA damage repair machinery. Three shelterin components (TRF1, TRF2, POT1) bind DNA and 3 components (TIN2, RAP1, TPP1) are recruited by these DNA binding factors. TIN2 binds directly to TRF1 and TRF2 and stabilizes TRF2 complex-telomere binding by tethering it to the TRF1 complex. TRF1 activity at telomeres is regulated in part by selective ubiquitination and degradation. Ubiquitination of TRF1 is mediated by Fbx4, which binds TRF1 in the TRFH domain, via a small GTPase module. When bound to telomeres, TIN2 acts to protect TRF1 from SCF-Fbx4 mediated ubiquitination. F-box proteins act in substrate recognition as part of SCF complexes (SCF: Skp1-Cul1-Rbx1-F- box protein). Tankyrase-mediated ADP-ribosylation releases TRF1 from telomeres, rendering them susceptible to ubiquitination and degradation, promoting telomere elongation. TIN2 also binds TPP1, which recruits POT1 to telomeres.


Pssm-ID: 240666 [Multi-domain]  Cd Length: 108  Bit Score: 126.48  E-value: 5.76e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 151101235 249 HPEPLAGRHFNLAPLGRRRVQSQWASTRGGHKERPTVMLFPFRNLGSPTQVISK-PESKEEHAIYTADLAMGTRAASTGK 327
Cdd:cd11741    1 PPSSLTGRGFGLAPLSKRKSQSRWPSAPRSHKERPTTMLPPRRSTKLPTQKPSRsPASREEHGNNDAAASMGTRSSSTPK 80

                         90       100
                 ....*....|....*....|....*...
gi 151101235 328 SKSP--CQTLGGRALKENPVDLPATEQK 353
Cdd:cd11741   81 AKSPdpQDPLGRRSQERPPCSSAASNSF 108
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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