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Conserved domains on  [gi|149131|gb|AAA98150|]
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isopentenyl transferase (plasmid) [Plasmid pTiS4]

Protein Classification

isopentenyl transferase family protein( domain architecture ID 10484361)

isopentenyl transferase family protein similar to adenylate dimethylallyltransferase that transfers dimethylallyl groups to AMP as part of the biosynthesis of cytokinin phytohormones

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
IPT pfam01745
Isopentenyl transferase; Isopentenyl transferase / dimethylallyl transferase synthesizes ...
 1-233 2.30e-140

Isopentenyl transferase; Isopentenyl transferase / dimethylallyl transferase synthesizes isopentenyladensosine 5'-monophosphate, a cytokinin that induces shoot formation on host plants infected with the Ti plasmid.


:

Pssm-ID: 366786  Cd Length: 232  Bit Score: 392.53  E-value: 2.30e-140
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131       1 MEAHLIFGPTSTGKTSVAIALAKRTGFPVIVLDRIQCYSQLSVGGGRPSAAEFQGTRRIYLIEGSLDEGVISAERAHECL 80
Cdd:pfam01745   1 MGLYLIWGATCTGKTAEAIALAKETGWPVIVLDRVQCCSQLATGSGRPLPAELQGTRRIYLDNRPLSEGIIDAEEAHDRL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131      81 VAAVEAHKPEGGVILEGGSISLFKRMAQSSYWNCGFTWHVTRLHLGGEEIFLAAAKKRINQMMQPDEQGNSFLGELVSVW 160
Cdd:pfam01745  81 IAEVTSHKDEGGVILEGGSISLLKRMAQSPYWNAGFPWHVKRMRLPDRDVFLAQAKARVRQMLRPDSGGPSLLDELAELW 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 149131     161 KTTALRATLEGICGYRYAIEFAGKQGLEMDALTSLNRRQLEQLVHGMAHEYLCYARQQEQELPLPSlAGGEGP 233
Cdd:pfam01745 161 VLPAARPILEDIDGYRCAIDFARKHQLTIDQLTNIDADQLEELIEGIAQEYLEHALWQEQEFPLTP-EWGAGP 232
 
Name Accession Description Interval E-value
IPT pfam01745
Isopentenyl transferase; Isopentenyl transferase / dimethylallyl transferase synthesizes ...
 1-233 2.30e-140

Isopentenyl transferase; Isopentenyl transferase / dimethylallyl transferase synthesizes isopentenyladensosine 5'-monophosphate, a cytokinin that induces shoot formation on host plants infected with the Ti plasmid.


Pssm-ID: 366786  Cd Length: 232  Bit Score: 392.53  E-value: 2.30e-140
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131       1 MEAHLIFGPTSTGKTSVAIALAKRTGFPVIVLDRIQCYSQLSVGGGRPSAAEFQGTRRIYLIEGSLDEGVISAERAHECL 80
Cdd:pfam01745   1 MGLYLIWGATCTGKTAEAIALAKETGWPVIVLDRVQCCSQLATGSGRPLPAELQGTRRIYLDNRPLSEGIIDAEEAHDRL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131      81 VAAVEAHKPEGGVILEGGSISLFKRMAQSSYWNCGFTWHVTRLHLGGEEIFLAAAKKRINQMMQPDEQGNSFLGELVSVW 160
Cdd:pfam01745  81 IAEVTSHKDEGGVILEGGSISLLKRMAQSPYWNAGFPWHVKRMRLPDRDVFLAQAKARVRQMLRPDSGGPSLLDELAELW 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 149131     161 KTTALRATLEGICGYRYAIEFAGKQGLEMDALTSLNRRQLEQLVHGMAHEYLCYARQQEQELPLPSlAGGEGP 233
Cdd:pfam01745 161 VLPAARPILEDIDGYRCAIDFARKHQLTIDQLTNIDADQLEELIEGIAQEYLEHALWQEQEFPLTP-EWGAGP 232
MiaA COG0324
tRNA A37 N6-isopentenylltransferase MiaA [Translation, ribosomal structure and biogenesis]; ...
 5-99 2.97e-08

tRNA A37 N6-isopentenylltransferase MiaA [Translation, ribosomal structure and biogenesis]; tRNA A37 N6-isopentenylltransferase MiaA is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440093  Cd Length: 306  Bit Score: 53.14  E-value: 2.97e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131     5 LIFGPTSTGKTSVAIALAKRTGFPVIVLDRIQCYSQLSVGGGRPSAAEfqgTRRI--YLIegsldeGVIS---------- 72
Cdd:COG0324   6 VIVGPTASGKTALAIELAKRLGGEIISADSMQVYRGMDIGTAKPTAEE---RAGVphHLI------DILDpdeeysvadf 76

                        90       100
                ....*....|....*....|....*..
gi 149131    73 AERAHECLVAAVEAHKPeggVILEGGS 99
Cdd:COG0324  77 QRDARAAIAEILARGKL---PILVGGT 100
PLN02840 PLN02840
tRNA dimethylallyltransferase
 5-57 3.22e-07

tRNA dimethylallyltransferase


Pssm-ID: 215451  Cd Length: 421  Bit Score: 50.20  E-value: 3.22e-07
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 149131      5 LIFGPTSTGKTSVAIALAKRTGFPVIVLDRIQCYSQLSVGGGRPSAAEFQGTR 57
Cdd:PLN02840  25 VISGPTGAGKSRLALELAKRLNGEIISADSVQVYRGLDVGSAKPSLSERKEVP 77
miaA TIGR00174
tRNA dimethylallyltransferase; Alternate names include delta(2)-isopentenylpyrophosphate ...
 6-104 2.97e-06

tRNA dimethylallyltransferase; Alternate names include delta(2)-isopentenylpyrophosphate transferase, IPP transferase, 2-methylthio-N6-isopentyladenosine tRNA modification enzyme. Catalyzes the first step in the modification of an adenosine near the anticodon to 2-methylthio-N6-isopentyladenosine. Understanding of substrate specificity has changed. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 213512  Cd Length: 287  Bit Score: 47.00  E-value: 2.97e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131       6 IFGPTSTGKTSVAIALAKRTGFPVIVLDRIQCYSQLSVGGGRPSAAEFQGTRRiYLIEgSLD--EGVISAERAHECLVAA 83
Cdd:TIGR00174   4 LMGPTASGKSQLSIQLAQKLNAEIISVDSMQIYKGMDIGTAKPSLQEREGIPH-HLID-ILDpsESYSAADFQTQALNAI 81

                          90       100
                  ....*....|....*....|.
gi 149131      84 VEAHKPEGGVILEGGSISLFK 104
Cdd:TIGR00174  82 ADITARGKIPLLVGGTGLYLK 102
AAA smart00382
ATPases associated with a variety of cellular activities; AAA - ATPases associated with a ...
 5-62 1.69e-03

ATPases associated with a variety of cellular activities; AAA - ATPases associated with a variety of cellular activities. This profile/alignment only detects a fraction of this vast family. The poorly conserved N-terminal helix is missing from the alignment.


Pssm-ID: 214640 [Multi-domain]  Cd Length: 148  Bit Score: 37.74  E-value: 1.69e-03
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 149131        5 LIFGPTSTGKTSVAIALAK---RTGFPVIVLD----RIQCYSQLSVGGGRPSAAEFQGTRRIYLI 62
Cdd:smart00382   6 LIVGPPGSGKTTLARALARelgPPGGGVIYIDgediLEEVLDQLLLIIVGGKKASGSGELRLRLA 70
AAA cd00009
The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily ...
 5-68 4.34e-03

The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily represents an ancient group of ATPases belonging to the ASCE (for additional strand, catalytic E) division of the P-loop NTPase fold. The ASCE division also includes ABC, RecA-like, VirD4-like, PilT-like, and SF1/2 helicases. Members of the AAA+ ATPases function as molecular chaperons, ATPase subunits of proteases, helicases, or nucleic-acid stimulated ATPases. The AAA+ proteins contain several distinct features in addition to the conserved alpha-beta-alpha core domain structure and the Walker A and B motifs of the P-loop NTPases.


Pssm-ID: 99707 [Multi-domain]  Cd Length: 151  Bit Score: 36.74  E-value: 4.34e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131     5 LIFGPTSTGKTSVAIALAK---RTGFPVIVLDRIQCYSQLSVGGGRPSAAEFQGTRRIYLIEGS---LDE 68
Cdd:cd00009  23 LLYGPPGTGKTTLARAIANelfRPGAPFLYLNASDLLEGLVVAELFGHFLVRLLFELAEKAKPGvlfIDE 92
 
Name Accession Description Interval E-value
IPT pfam01745
Isopentenyl transferase; Isopentenyl transferase / dimethylallyl transferase synthesizes ...
 1-233 2.30e-140

Isopentenyl transferase; Isopentenyl transferase / dimethylallyl transferase synthesizes isopentenyladensosine 5'-monophosphate, a cytokinin that induces shoot formation on host plants infected with the Ti plasmid.


Pssm-ID: 366786  Cd Length: 232  Bit Score: 392.53  E-value: 2.30e-140
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131       1 MEAHLIFGPTSTGKTSVAIALAKRTGFPVIVLDRIQCYSQLSVGGGRPSAAEFQGTRRIYLIEGSLDEGVISAERAHECL 80
Cdd:pfam01745   1 MGLYLIWGATCTGKTAEAIALAKETGWPVIVLDRVQCCSQLATGSGRPLPAELQGTRRIYLDNRPLSEGIIDAEEAHDRL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131      81 VAAVEAHKPEGGVILEGGSISLFKRMAQSSYWNCGFTWHVTRLHLGGEEIFLAAAKKRINQMMQPDEQGNSFLGELVSVW 160
Cdd:pfam01745  81 IAEVTSHKDEGGVILEGGSISLLKRMAQSPYWNAGFPWHVKRMRLPDRDVFLAQAKARVRQMLRPDSGGPSLLDELAELW 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 149131     161 KTTALRATLEGICGYRYAIEFAGKQGLEMDALTSLNRRQLEQLVHGMAHEYLCYARQQEQELPLPSlAGGEGP 233
Cdd:pfam01745 161 VLPAARPILEDIDGYRCAIDFARKHQLTIDQLTNIDADQLEELIEGIAQEYLEHALWQEQEFPLTP-EWGAGP 232
MiaA COG0324
tRNA A37 N6-isopentenylltransferase MiaA [Translation, ribosomal structure and biogenesis]; ...
 5-99 2.97e-08

tRNA A37 N6-isopentenylltransferase MiaA [Translation, ribosomal structure and biogenesis]; tRNA A37 N6-isopentenylltransferase MiaA is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440093  Cd Length: 306  Bit Score: 53.14  E-value: 2.97e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131     5 LIFGPTSTGKTSVAIALAKRTGFPVIVLDRIQCYSQLSVGGGRPSAAEfqgTRRI--YLIegsldeGVIS---------- 72
Cdd:COG0324   6 VIVGPTASGKTALAIELAKRLGGEIISADSMQVYRGMDIGTAKPTAEE---RAGVphHLI------DILDpdeeysvadf 76

                        90       100
                ....*....|....*....|....*..
gi 149131    73 AERAHECLVAAVEAHKPeggVILEGGS 99
Cdd:COG0324  77 QRDARAAIAEILARGKL---PILVGGT 100
PLN02840 PLN02840
tRNA dimethylallyltransferase
 5-57 3.22e-07

tRNA dimethylallyltransferase


Pssm-ID: 215451  Cd Length: 421  Bit Score: 50.20  E-value: 3.22e-07
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 149131      5 LIFGPTSTGKTSVAIALAKRTGFPVIVLDRIQCYSQLSVGGGRPSAAEFQGTR 57
Cdd:PLN02840  25 VISGPTGAGKSRLALELAKRLNGEIISADSVQVYRGLDVGSAKPSLSERKEVP 77
miaA TIGR00174
tRNA dimethylallyltransferase; Alternate names include delta(2)-isopentenylpyrophosphate ...
 6-104 2.97e-06

tRNA dimethylallyltransferase; Alternate names include delta(2)-isopentenylpyrophosphate transferase, IPP transferase, 2-methylthio-N6-isopentyladenosine tRNA modification enzyme. Catalyzes the first step in the modification of an adenosine near the anticodon to 2-methylthio-N6-isopentyladenosine. Understanding of substrate specificity has changed. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 213512  Cd Length: 287  Bit Score: 47.00  E-value: 2.97e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131       6 IFGPTSTGKTSVAIALAKRTGFPVIVLDRIQCYSQLSVGGGRPSAAEFQGTRRiYLIEgSLD--EGVISAERAHECLVAA 83
Cdd:TIGR00174   4 LMGPTASGKSQLSIQLAQKLNAEIISVDSMQIYKGMDIGTAKPSLQEREGIPH-HLID-ILDpsESYSAADFQTQALNAI 81

                          90       100
                  ....*....|....*....|.
gi 149131      84 VEAHKPEGGVILEGGSISLFK 104
Cdd:TIGR00174  82 ADITARGKIPLLVGGTGLYLK 102
COG1223 COG1223
Predicted ATPase, AAA+ superfamily [General function prediction only];
5-32 5.51e-04

Predicted ATPase, AAA+ superfamily [General function prediction only];


Pssm-ID: 440836 [Multi-domain]  Cd Length: 246  Bit Score: 39.87  E-value: 5.51e-04
                        10        20
                ....*....|....*....|....*...
gi 149131     5 LIFGPTSTGKTSVAIALAKRTGFPVIVL 32
Cdd:COG1223  39 LFYGPPGTGKTMLAEALAGELKLPLLTV 66
SpoVK COG0464
AAA+-type ATPase, SpoVK/Ycf46/Vps4 family [Cell wall/membrane/envelope biogenesis, Cell cycle ...
 5-34 8.52e-04

AAA+-type ATPase, SpoVK/Ycf46/Vps4 family [Cell wall/membrane/envelope biogenesis, Cell cycle control, cell division, chromosome partitioning, Signal transduction mechanisms];


Pssm-ID: 440232 [Multi-domain]  Cd Length: 397  Bit Score: 39.89  E-value: 8.52e-04
                        10        20        30
                ....*....|....*....|....*....|
gi 149131     5 LIFGPTSTGKTSVAIALAKRTGFPVIVLDR 34
Cdd:COG0464 195 LLYGPPGTGKTLLARALAGELGLPLIEVDL 224
AAA pfam00004
ATPase family associated with various cellular activities (AAA); AAA family proteins often ...
 5-33 1.61e-03

ATPase family associated with various cellular activities (AAA); AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes.


Pssm-ID: 459627 [Multi-domain]  Cd Length: 130  Bit Score: 37.57  E-value: 1.61e-03
                          10        20
                  ....*....|....*....|....*....
gi 149131       5 LIFGPTSTGKTSVAIALAKRTGFPVIVLD 33
Cdd:pfam00004   2 LLYGPPGTGKTTLAKAVAKELGAPFIEIS 30
AAA smart00382
ATPases associated with a variety of cellular activities; AAA - ATPases associated with a ...
 5-62 1.69e-03

ATPases associated with a variety of cellular activities; AAA - ATPases associated with a variety of cellular activities. This profile/alignment only detects a fraction of this vast family. The poorly conserved N-terminal helix is missing from the alignment.


Pssm-ID: 214640 [Multi-domain]  Cd Length: 148  Bit Score: 37.74  E-value: 1.69e-03
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 149131        5 LIFGPTSTGKTSVAIALAK---RTGFPVIVLD----RIQCYSQLSVGGGRPSAAEFQGTRRIYLI 62
Cdd:smart00382   6 LIVGPPGSGKTTLARALARelgPPGGGVIYIDgediLEEVLDQLLLIIVGGKKASGSGELRLRLA 70
AAA cd00009
The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily ...
 5-68 4.34e-03

The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily represents an ancient group of ATPases belonging to the ASCE (for additional strand, catalytic E) division of the P-loop NTPase fold. The ASCE division also includes ABC, RecA-like, VirD4-like, PilT-like, and SF1/2 helicases. Members of the AAA+ ATPases function as molecular chaperons, ATPase subunits of proteases, helicases, or nucleic-acid stimulated ATPases. The AAA+ proteins contain several distinct features in addition to the conserved alpha-beta-alpha core domain structure and the Walker A and B motifs of the P-loop NTPases.


Pssm-ID: 99707 [Multi-domain]  Cd Length: 151  Bit Score: 36.74  E-value: 4.34e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 149131     5 LIFGPTSTGKTSVAIALAK---RTGFPVIVLDRIQCYSQLSVGGGRPSAAEFQGTRRIYLIEGS---LDE 68
Cdd:cd00009  23 LLYGPPGTGKTTLARAIANelfRPGAPFLYLNASDLLEGLVVAELFGHFLVRLLFELAEKAKPGvlfIDE 92
AAA_28 pfam13521
AAA domain;
6-30 4.52e-03

AAA domain;


Pssm-ID: 433278 [Multi-domain]  Cd Length: 164  Bit Score: 36.86  E-value: 4.52e-03
                          10        20
                  ....*....|....*....|....*
gi 149131       6 IFGPTSTGKTSVAIALAKRTGFPVI 30
Cdd:pfam13521   4 ITGGPSTGKTTLAEALAARFGYPVV 28
COG0645 COG0645
Predicted kinase, contains AAA domain [General function prediction only];
4-71 7.48e-03

Predicted kinase, contains AAA domain [General function prediction only];


Pssm-ID: 440410 [Multi-domain]  Cd Length: 164  Bit Score: 36.04  E-value: 7.48e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 149131     4 HLIFGPTSTGKTSVAIALAKRTGFPVIVLDRIqcYSQLsVGGGRPSAAEFQG-TRRIY-----LIEGSLDEG--VI 71
Cdd:COG0645   2 ILVCGLPGSGKSTLARALAERLGAVRLRSDVV--RKRL-FGAGLAPLERSPEaTARTYarllaLARELLAAGrsVI 74
RecA-like_protease cd19481
proteases similar to RecA; RecA-like NTPases. This family includes the NTP binding domain of ...
 5-33 8.26e-03

proteases similar to RecA; RecA-like NTPases. This family includes the NTP binding domain of F1 and V1 H(+)ATPases, DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases. This group also includes bacterial conjugation proteins and related DNA transfer proteins involved in type II and type IV secretion.


Pssm-ID: 410889 [Multi-domain]  Cd Length: 158  Bit Score: 35.72  E-value: 8.26e-03
                        10        20
                ....*....|....*....|....*....
gi 149131     5 LIFGPTSTGKTSVAIALAKRTGFPVIVLD 33
Cdd:cd19481  30 LLYGPPGTGKTLLAKALAGELGLPLIVVK 58
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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