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Conserved domains on  [gi|1465249563|gb|RFZ18324|]
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Resuscitation-promoting factor RpfA precursor [Mycobacterium marinum]

Protein Classification

transglycosylase family protein( domain architecture ID 10535629)

transglycosylase family protein has peptidoglycan (PG) hydrolytic activity, similar to Mycobacterium tuberculosis Rpf resuscitation-promoting factors and Staphylococcus aureus transglycosylase SceD

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Transglycosylas pfam06737
Transglycosylase-like domain; This family of proteins are very likely to act as ...
 40-114 2.05e-42

Transglycosylase-like domain; This family of proteins are very likely to act as transglycosylase enzymes related to pfam00062 and pfam01464. These other families are weakly matched by this family, and include the known active site residues.


:

Pssm-ID: 399604 [Multi-domain]  Cd Length: 75  Bit Score: 143.32  E-value: 2.05e-42
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1465249563  40 TDGEWDQVARCESGGNWAINTGNGYYGGVQFAPSTWASHGGGEYAPSAQLASREEQIAVAERVLATQGRGAWPVC 114
Cdd:pfam06737   1 DNSNWDAIAQCESGGNWAINTGNGYYGGLQFSQSTWDAYGGLGYAPSAAQASREQQIAVAEKLLATQGAGAWPTC 75
 
Name Accession Description Interval E-value
Transglycosylas pfam06737
Transglycosylase-like domain; This family of proteins are very likely to act as ...
 40-114 2.05e-42

Transglycosylase-like domain; This family of proteins are very likely to act as transglycosylase enzymes related to pfam00062 and pfam01464. These other families are weakly matched by this family, and include the known active site residues.


Pssm-ID: 399604 [Multi-domain]  Cd Length: 75  Bit Score: 143.32  E-value: 2.05e-42
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1465249563  40 TDGEWDQVARCESGGNWAINTGNGYYGGVQFAPSTWASHGGGEYAPSAQLASREEQIAVAERVLATQGRGAWPVC 114
Cdd:pfam06737   1 DNSNWDAIAQCESGGNWAINTGNGYYGGLQFSQSTWDAYGGLGYAPSAAQASREQQIAVAEKLLATQGAGAWPTC 75
RPF cd13925
core lysozyme-like domain of resuscitation-promoting factor proteins; Resuscitation-promoting ...
 44-114 9.30e-28

core lysozyme-like domain of resuscitation-promoting factor proteins; Resuscitation-promoting factor (RPF) proteins, found in various (G+C)-rich Gram-positive bacteria, act to reactivate cultures from stationary phase. This protein shares elements of the structural core of lysozyme and related proteins. Furthermore, it shares a conserved active site glutamate which is required for activity, and has a polysaccharide binding cleft that corresponds to the peptidoglycan binding cleft of lysozyme. Muralytic activity of Rpf in Micrococcus luteus correlates with resuscitation, supporting a mechanism dependent on cleavage of peptidoglycan by RPF.


Pssm-ID: 381607 [Multi-domain]  Cd Length: 71  Bit Score: 104.38  E-value: 9.30e-28
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1465249563  44 WDQVARCESGGNW-AINTGNGYYGGVQFAPSTWASHGGGEYapsAQLASREEQIAVAERVLATQGRGAWPVC 114
Cdd:cd13925     3 WDAIAQCESGGNWnAVNTGNGYYGGLQFLQGTWKSVGGLGY---PDDATRAEQITRAERLYARQGAGAWPCC 71
 
Name Accession Description Interval E-value
Transglycosylas pfam06737
Transglycosylase-like domain; This family of proteins are very likely to act as ...
 40-114 2.05e-42

Transglycosylase-like domain; This family of proteins are very likely to act as transglycosylase enzymes related to pfam00062 and pfam01464. These other families are weakly matched by this family, and include the known active site residues.


Pssm-ID: 399604 [Multi-domain]  Cd Length: 75  Bit Score: 143.32  E-value: 2.05e-42
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1465249563  40 TDGEWDQVARCESGGNWAINTGNGYYGGVQFAPSTWASHGGGEYAPSAQLASREEQIAVAERVLATQGRGAWPVC 114
Cdd:pfam06737   1 DNSNWDAIAQCESGGNWAINTGNGYYGGLQFSQSTWDAYGGLGYAPSAAQASREQQIAVAEKLLATQGAGAWPTC 75
RPF cd13925
core lysozyme-like domain of resuscitation-promoting factor proteins; Resuscitation-promoting ...
 44-114 9.30e-28

core lysozyme-like domain of resuscitation-promoting factor proteins; Resuscitation-promoting factor (RPF) proteins, found in various (G+C)-rich Gram-positive bacteria, act to reactivate cultures from stationary phase. This protein shares elements of the structural core of lysozyme and related proteins. Furthermore, it shares a conserved active site glutamate which is required for activity, and has a polysaccharide binding cleft that corresponds to the peptidoglycan binding cleft of lysozyme. Muralytic activity of Rpf in Micrococcus luteus correlates with resuscitation, supporting a mechanism dependent on cleavage of peptidoglycan by RPF.


Pssm-ID: 381607 [Multi-domain]  Cd Length: 71  Bit Score: 104.38  E-value: 9.30e-28
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1465249563  44 WDQVARCESGGNW-AINTGNGYYGGVQFAPSTWASHGGGEYapsAQLASREEQIAVAERVLATQGRGAWPVC 114
Cdd:cd13925     3 WDAIAQCESGGNWnAVNTGNGYYGGLQFLQGTWKSVGGLGY---PDDATRAEQITRAERLYARQGAGAWPCC 71
Lyz-like cd00442
lysozyme-like domains; This family contains several members, including soluble lytic ...
 43-101 1.15e-07

lysozyme-like domains; This family contains several members, including soluble lytic transglycosylases (SLT), goose egg-white lysozymes (GEWL), hen egg-white lysozymes (HEWL), chitinases, bacteriophage lambda lysozymes, endolysins, autolysins, chitosanases, and pesticin. Typical members are involved in the hydrolysis of beta-1,4- linked polysaccharides.


Pssm-ID: 381596 [Multi-domain]  Cd Length: 59  Bit Score: 48.17  E-value: 1.15e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1465249563  43 EWDQVARCESGGNW--AINTGNGYYGGVQFAPSTWASHGGgeyAPSAQLASREEQIAVAER 101
Cdd:cd00442     1 VLAAIIGQESGGNKpaNAGSGSGAAGLFQFMPGTWKAYGK---NSSSDLNDPEASIEAAAK 58
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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