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Conserved domains on  [gi|1347645565|gb|PPR55470|]
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UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [Alphaproteobacteria bacterium MarineAlpha5_Bin1]

Protein Classification

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase( domain architecture ID 11433680)

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein

CATH:  3.90.190.20|3.40.1190.10|3.40.1390.10
EC:  6.3.2.10
Gene Ontology:  GO:0005524|GO:0009252|GO:0047480|GO:0071555
PubMed:  16934839
SCOP:  4000234

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MurF COG0770
UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 28-463 3.95e-92

UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl pentapeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


:

Pssm-ID: 440533 [Multi-domain]  Cd Length: 451  Bit Score: 286.61  E-value: 3.95e-92
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  28 EINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFifnKILVVDDILTFLLSTAKIKRNIFKGKVIGITGSV 107
Cdd:COG0770    33 KIKPGDLFVALKGERFDGHDFVAQALAKGAAAALVSRPLPADL---PLIVVDDTLKALQQLAAAHRARFNIPVIAITGSN 109

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 108 GKTTLKENLKYFISQTSTVSVSIKSYNNYLGVLISLININLSSSFAIFEIGTNNFEEIKNLTSIIMPSQAIITNILPTHL 187
Cdd:COG0770   110 GKTTTKEMLAAVLSTKGKVLATPGNFNNEIGVPLTLLRLPEDHEFAVLEMGMNHPGEIAYLARIARPDIAVITNIGPAHL 189

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 188 ENFKNTRNIAIEKSDIFspEYNPNIELLVLsqnNTDETYISDLAlKENISNIITFGKTNNQNYRIEKINKIDDKT-FEIL 266
Cdd:COG0770   190 EGFGSLEGIARAKGEIF--EGLPPGGVAVL---NADDPLLAALA-ERAKARVLTFGLSEDADVRAEDIELDEDGTrFTLH 263

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 267 ICNNTTTYKFeinkNQLSRinnilisliIFVYN-----------KLDITTFISSTKNVELVEGRgLESKIFINNKIInfI 335
Cdd:COG0770   264 TPGGELEVTL----PLPGR---------HNVSNalaaaavalalGLDLEEIAAGLAAFQPVKGR-LEVIEGAGGVTL--I 327

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 336 DETYNASPSTMRMCIDEFSNIKTmkNQKKILILGDMKELGEEALGFHLEILEYIITLDLNNVIICGELMKSALNKLSKKd 415
Cdd:COG0770   328 DDSYNANPDSMKAALDVLAQLPG--GGRRIAVLGDMLELGEESEELHREVGELAAELGIDRLFTVGELARAIAEAAGGE- 404

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*...
gi 1347645565 416 lKIELMLNEKLIIKYLKKTLNNNDILLIKGSNSSLTNKIGKILLKKGE 463
Cdd:COG0770   405 -RAEHFEDKEELLAALKALLRPGDVVLVKGSRGMGLERVVEALKEGAA 451
 
Name Accession Description Interval E-value
MurF COG0770
UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 28-463 3.95e-92

UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl pentapeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440533 [Multi-domain]  Cd Length: 451  Bit Score: 286.61  E-value: 3.95e-92
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  28 EINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFifnKILVVDDILTFLLSTAKIKRNIFKGKVIGITGSV 107
Cdd:COG0770    33 KIKPGDLFVALKGERFDGHDFVAQALAKGAAAALVSRPLPADL---PLIVVDDTLKALQQLAAAHRARFNIPVIAITGSN 109

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 108 GKTTLKENLKYFISQTSTVSVSIKSYNNYLGVLISLININLSSSFAIFEIGTNNFEEIKNLTSIIMPSQAIITNILPTHL 187
Cdd:COG0770   110 GKTTTKEMLAAVLSTKGKVLATPGNFNNEIGVPLTLLRLPEDHEFAVLEMGMNHPGEIAYLARIARPDIAVITNIGPAHL 189

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 188 ENFKNTRNIAIEKSDIFspEYNPNIELLVLsqnNTDETYISDLAlKENISNIITFGKTNNQNYRIEKINKIDDKT-FEIL 266
Cdd:COG0770   190 EGFGSLEGIARAKGEIF--EGLPPGGVAVL---NADDPLLAALA-ERAKARVLTFGLSEDADVRAEDIELDEDGTrFTLH 263

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 267 ICNNTTTYKFeinkNQLSRinnilisliIFVYN-----------KLDITTFISSTKNVELVEGRgLESKIFINNKIInfI 335
Cdd:COG0770   264 TPGGELEVTL----PLPGR---------HNVSNalaaaavalalGLDLEEIAAGLAAFQPVKGR-LEVIEGAGGVTL--I 327

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 336 DETYNASPSTMRMCIDEFSNIKTmkNQKKILILGDMKELGEEALGFHLEILEYIITLDLNNVIICGELMKSALNKLSKKd 415
Cdd:COG0770   328 DDSYNANPDSMKAALDVLAQLPG--GGRRIAVLGDMLELGEESEELHREVGELAAELGIDRLFTVGELARAIAEAAGGE- 404

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*...
gi 1347645565 416 lKIELMLNEKLIIKYLKKTLNNNDILLIKGSNSSLTNKIGKILLKKGE 463
Cdd:COG0770   405 -RAEHFEDKEELLAALKALLRPGDVVLVKGSRGMGLERVVEALKEGAA 451
murF TIGR01143
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the ...
 24-458 2.25e-76

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the strictly bacterial MurF gene of peptidoglycan biosynthesis. This enzyme is almost always UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanyl ligase, but in a few species, MurE adds lysine rather than diaminopimelate. This enzyme acts on the product from MurE activity, and so is also subfamily rather than equivalog. Staphylococcus aureus is an example of species in this MurF protein would differ. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273468 [Multi-domain]  Cd Length: 417  Bit Score: 244.87  E-value: 2.25e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  24 ISSLEINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFIfNKILVVDDILTFLLSTAKIKRNIFKGKVIGI 103
Cdd:TIGR01143   1 TDSRAIKPGDLFIALKGERFDGHDFVEQALAAGAVAVVVDREVGPDNG-LPQILVDDTLEALQALARAKRAKFSGKVIGI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 104 TGSVGKTTLKENLKYFISQTSTVSVSIKSYNNYLGVLISLININLSSSFAIFEIGTNNFEEIKNLTSIIMPSQAIITNIL 183
Cdd:TIGR01143  80 TGSSGKTTTKEMLAAILSHKYKVFATPGNFNNEIGLPLTLLRAPGDHDYAVLEMGASHPGEIAYLAEIAKPDIAVITNIG 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 184 PTHLENFKNTRNIAIEKSDIFspEYNPNIELLVLsqnNTDETYISDLALKENISNIITFGKtNNQNYRIEKI--NKIDDK 261
Cdd:TIGR01143 160 PAHLEGFGSLEGIAEAKGEIL--QGLKENGIAVI---NADDPAFADLAKRLPNRNILSFGF-EGGDFVAKDIsySALGST 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 262 TFEILICNNtttyKFEINKNQLSRinnilisliIFVYN-----------KLDITTFISSTKNVELVEGRGleSKIFINNK 330
Cdd:TIGR01143 234 SFTLVAPGG----EFEVSLPLLGR---------HNVMNalaaaalalelGIPLEEIAEGLAELKLVKGRF--EVQTKNGL 298

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 331 IInfIDETYNASPSTMRMCIDEFSNIKTmknqKKILILGDMKELGEEALGFHLEILEYIITLDLNNVIICGELMKSALNK 410
Cdd:TIGR01143 299 TL--IDDTYNANPDSMRAALDALARFPG----KKILVLGDMAELGEYSEELHAEVGRYANSLGIDLVFLVGEEAAVIYDS 372

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*...
gi 1347645565 411 LSKkdlKIELMLNEKLIIKYLKKTLNNNDILLIKGSNSsltNKIGKIL 458
Cdd:TIGR01143 373 FGK---QGKHFADKDELLAFLKTLVRKGDVVLVKGSRS---VKLEKVV 414
PRK11929 PRK11929
bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE ...
 20-448 5.00e-57

bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF;


Pssm-ID: 237025 [Multi-domain]  Cd Length: 958  Bit Score: 203.01  E-value: 5.00e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  20 GKAKISSLEINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFIfnKILVVDDILTFLLSTAKIKRNIFKGK 99
Cdd:PRK11929  527 GAVSTDSRSVGRGELFVALRGENFDGHDYLPQAFAAGACAAVVERQVADVDL--PQIVVDDTRAALGRLATAWRARFSLP 604

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 100 VIGITGSVGKTTLKENLKYFISQ---TSTVSVSIKSYNNYLGVLISLININLSSSFAIFEIGTNNFEEIKNLTSIIMPSQ 176
Cdd:PRK11929  605 VVAITGSNGKTTTKEMIAAILAAwqgEDRVLATEGNFNNEIGVPLTLLRLRAQHRAAVFELGMNHPGEIAYLAAIAAPTV 684

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 177 AIITNILPTHLENFKNTRNIAIEKSDIFSpeYNPNIELLVLsqnNTDETYISDLALKENISNIITFGKTNNQNYRIEKIN 256
Cdd:PRK11929  685 ALVTNAQREHQEFMHSVEAVARAKGEIIA--ALPEDGVAVV---NGDDPYTAIWAKLAGARRVLRFGLQPGADVYAEKIA 759

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 257 KiDDKTFE-----ILICNNTTTYKFEIN---KNQLSRINNILISLIIFvynKLDITTFISSTKNVELVEGRGlESKIFIN 328
Cdd:PRK11929  760 K-DISVGEaggtrCQVVTPAGSAEVYLPligEHNLRNALAAIACALAA---GASLKQIRAGLERFQPVAGRM-QRRRLSC 834

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 329 NKIInfIDETYNASPSTMRMCIDefsNIKTMKNQKKILILGDMKELGEEALGFHLEILEYIITLDLNNVIICGE------ 402
Cdd:PRK11929  835 GTRI--IDDTYNANPDSMRAAID---VLAELPNGPRALVLGDMLELGDNGPAMHREVGKYARQLGIDALITLGEaardaa 909

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*...
gi 1347645565 403 --LMKSALNKLSKKDlkielmlnekLIIKYLKKTLNNNDILLIKGSNS 448
Cdd:PRK11929  910 aaFGAGARGVCASVD----------EIIAALRGALPEGDSVLIKGSRF 947
Mur_ligase_M pfam08245
Mur ligase middle domain;
103-256 2.07e-13

Mur ligase middle domain;


Pssm-ID: 462409 [Multi-domain]  Cd Length: 199  Bit Score: 68.87  E-value: 2.07e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 103 ITGSVGKTTLKEN----LKYFISQTSTV----SVSIKSYNNYLGVLISLINI-NLSSSFAIFEIGTNNFEEiKNLTSIIM 173
Cdd:pfam08245   1 VTGTNGKTTTTELiaaiLSLAGGVIGTIgtyiGKSGNTTNNAIGLPLTLAEMvEAGAEYAVLEVSSHGLGE-GRLSGLLK 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 174 PSQAIITNILPTHLENFKNTRNIAIEKSDIFSPEYNPNIelLVLsqnNTDETYISDLA--LKENISNIITFGKTNNQNYR 251
Cdd:pfam08245  80 PDIAVFTNISPDHLDFHGTMENYAKAKAELFEGLPEDGI--AVI---NADDPYGAFLIakLKKAGVRVITYGIEGEADLR 154


                  ....*
gi 1347645565 252 IEKIN 256
Cdd:pfam08245 155 AANIE 159
 
Name Accession Description Interval E-value
MurF COG0770
UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 28-463 3.95e-92

UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl pentapeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440533 [Multi-domain]  Cd Length: 451  Bit Score: 286.61  E-value: 3.95e-92
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  28 EINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFifnKILVVDDILTFLLSTAKIKRNIFKGKVIGITGSV 107
Cdd:COG0770    33 KIKPGDLFVALKGERFDGHDFVAQALAKGAAAALVSRPLPADL---PLIVVDDTLKALQQLAAAHRARFNIPVIAITGSN 109

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 108 GKTTLKENLKYFISQTSTVSVSIKSYNNYLGVLISLININLSSSFAIFEIGTNNFEEIKNLTSIIMPSQAIITNILPTHL 187
Cdd:COG0770   110 GKTTTKEMLAAVLSTKGKVLATPGNFNNEIGVPLTLLRLPEDHEFAVLEMGMNHPGEIAYLARIARPDIAVITNIGPAHL 189

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 188 ENFKNTRNIAIEKSDIFspEYNPNIELLVLsqnNTDETYISDLAlKENISNIITFGKTNNQNYRIEKINKIDDKT-FEIL 266
Cdd:COG0770   190 EGFGSLEGIARAKGEIF--EGLPPGGVAVL---NADDPLLAALA-ERAKARVLTFGLSEDADVRAEDIELDEDGTrFTLH 263

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 267 ICNNTTTYKFeinkNQLSRinnilisliIFVYN-----------KLDITTFISSTKNVELVEGRgLESKIFINNKIInfI 335
Cdd:COG0770   264 TPGGELEVTL----PLPGR---------HNVSNalaaaavalalGLDLEEIAAGLAAFQPVKGR-LEVIEGAGGVTL--I 327

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 336 DETYNASPSTMRMCIDEFSNIKTmkNQKKILILGDMKELGEEALGFHLEILEYIITLDLNNVIICGELMKSALNKLSKKd 415
Cdd:COG0770   328 DDSYNANPDSMKAALDVLAQLPG--GGRRIAVLGDMLELGEESEELHREVGELAAELGIDRLFTVGELARAIAEAAGGE- 404

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*...
gi 1347645565 416 lKIELMLNEKLIIKYLKKTLNNNDILLIKGSNSSLTNKIGKILLKKGE 463
Cdd:COG0770   405 -RAEHFEDKEELLAALKALLRPGDVVLVKGSRGMGLERVVEALKEGAA 451
murF TIGR01143
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the ...
 24-458 2.25e-76

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the strictly bacterial MurF gene of peptidoglycan biosynthesis. This enzyme is almost always UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanyl ligase, but in a few species, MurE adds lysine rather than diaminopimelate. This enzyme acts on the product from MurE activity, and so is also subfamily rather than equivalog. Staphylococcus aureus is an example of species in this MurF protein would differ. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273468 [Multi-domain]  Cd Length: 417  Bit Score: 244.87  E-value: 2.25e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  24 ISSLEINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFIfNKILVVDDILTFLLSTAKIKRNIFKGKVIGI 103
Cdd:TIGR01143   1 TDSRAIKPGDLFIALKGERFDGHDFVEQALAAGAVAVVVDREVGPDNG-LPQILVDDTLEALQALARAKRAKFSGKVIGI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 104 TGSVGKTTLKENLKYFISQTSTVSVSIKSYNNYLGVLISLININLSSSFAIFEIGTNNFEEIKNLTSIIMPSQAIITNIL 183
Cdd:TIGR01143  80 TGSSGKTTTKEMLAAILSHKYKVFATPGNFNNEIGLPLTLLRAPGDHDYAVLEMGASHPGEIAYLAEIAKPDIAVITNIG 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 184 PTHLENFKNTRNIAIEKSDIFspEYNPNIELLVLsqnNTDETYISDLALKENISNIITFGKtNNQNYRIEKI--NKIDDK 261
Cdd:TIGR01143 160 PAHLEGFGSLEGIAEAKGEIL--QGLKENGIAVI---NADDPAFADLAKRLPNRNILSFGF-EGGDFVAKDIsySALGST 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 262 TFEILICNNtttyKFEINKNQLSRinnilisliIFVYN-----------KLDITTFISSTKNVELVEGRGleSKIFINNK 330
Cdd:TIGR01143 234 SFTLVAPGG----EFEVSLPLLGR---------HNVMNalaaaalalelGIPLEEIAEGLAELKLVKGRF--EVQTKNGL 298

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 331 IInfIDETYNASPSTMRMCIDEFSNIKTmknqKKILILGDMKELGEEALGFHLEILEYIITLDLNNVIICGELMKSALNK 410
Cdd:TIGR01143 299 TL--IDDTYNANPDSMRAALDALARFPG----KKILVLGDMAELGEYSEELHAEVGRYANSLGIDLVFLVGEEAAVIYDS 372

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*...
gi 1347645565 411 LSKkdlKIELMLNEKLIIKYLKKTLNNNDILLIKGSNSsltNKIGKIL 458
Cdd:TIGR01143 373 FGK---QGKHFADKDELLAFLKTLVRKGDVVLVKGSRS---VKLEKVV 414
PRK11929 PRK11929
bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE ...
 20-448 5.00e-57

bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF;


Pssm-ID: 237025 [Multi-domain]  Cd Length: 958  Bit Score: 203.01  E-value: 5.00e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  20 GKAKISSLEINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFIfnKILVVDDILTFLLSTAKIKRNIFKGK 99
Cdd:PRK11929  527 GAVSTDSRSVGRGELFVALRGENFDGHDYLPQAFAAGACAAVVERQVADVDL--PQIVVDDTRAALGRLATAWRARFSLP 604

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 100 VIGITGSVGKTTLKENLKYFISQ---TSTVSVSIKSYNNYLGVLISLININLSSSFAIFEIGTNNFEEIKNLTSIIMPSQ 176
Cdd:PRK11929  605 VVAITGSNGKTTTKEMIAAILAAwqgEDRVLATEGNFNNEIGVPLTLLRLRAQHRAAVFELGMNHPGEIAYLAAIAAPTV 684

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 177 AIITNILPTHLENFKNTRNIAIEKSDIFSpeYNPNIELLVLsqnNTDETYISDLALKENISNIITFGKTNNQNYRIEKIN 256
Cdd:PRK11929  685 ALVTNAQREHQEFMHSVEAVARAKGEIIA--ALPEDGVAVV---NGDDPYTAIWAKLAGARRVLRFGLQPGADVYAEKIA 759

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 257 KiDDKTFE-----ILICNNTTTYKFEIN---KNQLSRINNILISLIIFvynKLDITTFISSTKNVELVEGRGlESKIFIN 328
Cdd:PRK11929  760 K-DISVGEaggtrCQVVTPAGSAEVYLPligEHNLRNALAAIACALAA---GASLKQIRAGLERFQPVAGRM-QRRRLSC 834

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 329 NKIInfIDETYNASPSTMRMCIDefsNIKTMKNQKKILILGDMKELGEEALGFHLEILEYIITLDLNNVIICGE------ 402
Cdd:PRK11929  835 GTRI--IDDTYNANPDSMRAAID---VLAELPNGPRALVLGDMLELGDNGPAMHREVGKYARQLGIDALITLGEaardaa 909

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*...
gi 1347645565 403 --LMKSALNKLSKKDlkielmlnekLIIKYLKKTLNNNDILLIKGSNS 448
Cdd:PRK11929  910 aaFGAGARGVCASVD----------EIIAALRGALPEGDSVLIKGSRF 947
PRK14093 PRK14093
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase; ...
 24-461 1.92e-52

UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase; Provisional


Pssm-ID: 184501 [Multi-domain]  Cd Length: 479  Bit Score: 183.44  E-value: 1.92e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  24 ISSLEINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFIFN-KILVVDDILTFLLSTAKIKRNIFKGKVIG 102
Cdd:PRK14093   33 IDSRTLAPGDAYFAIKGDVHDGHAFVAAALKAGAALAVVERAQRDKFAADaPLLVVDDVLAALRDLGRAARARLEAKVIA 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 103 ITGSVGKTTLKENLKYFISQTSTVSVSIKSYNNYLGVLISLININLSSSFAIFEIGTNNFEEIKNLTSIIMPSQAIITNI 182
Cdd:PRK14093  113 VTGSVGKTSTKEALRGVLGAQGETHASVASFNNHWGVPLSLARCPADARFAVFEIGMNHAGEIEPLVKMVRPHVAIITTV 192

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 183 LPTHLENFKNTRNIAIEKSDIFSpeynpNIE---LLVLSQNNTDETYISDLALKENISNIITFGKTNNQNYRIEKIN-KI 258
Cdd:PRK14093  193 EPVHLEFFSGIEAIADAKAEIFT-----GLEpggAAVLNRDNPQFDRLAASARAAGIARIVSFGADEKADARLLDVAlHA 267

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 259 DDKTFEILICNNTTTYKFEINKNQLSRINNILISLIIFVYNKLDITTFISStkNVELVEGRGLESKIFINNKIINFIDET 338
Cdd:PRK14093  268 DCSAVHADILGHDVTYKLGMPGRHIAMNSLAVLAAAELAGADLALAALALS--QVQPAAGRGVRHTLEVGGGEATLIDES 345

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 339 YNASPSTMRMCIDEFSNIKTMKNQKKILILGDMKELGEEALGFHLEILEYIITLDLNNVIICGELMKSALNKLS---KKD 415
Cdd:PRK14093  346 YNANPASMAAALGVLGRAPVGPQGRRIAVLGDMLELGPRGPELHRGLAEAIRANAIDLVFCCGPLMRNLWDALSsgkRGG 425

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*.
gi 1347645565 416 LKIELMLNEKLIIKYLKktlnNNDILLIKGSNSSLTNKIGKILLKK 461
Cdd:PRK14093  426 YAEDAAALESQVVAAIR----AGDVIMVKGSLGSRMKTIVTALEKR 467
PRK11930 PRK11930
putative bifunctional UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase/alanine ...
 34-446 2.84e-44

putative bifunctional UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase/alanine racemase; Provisional


Pssm-ID: 237026 [Multi-domain]  Cd Length: 822  Bit Score: 165.90  E-value: 2.84e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  34 VFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFIFNK--ILVVDDILTFLLSTAKIKRNIFKGKVIGITGSVGKTT 111
Cdd:PRK11930   41 LFFALKGERNDGHRYIQELYEKGVRNFVVSEEKHPEESYPDanFLKVKDPLKALQELAAYHRSQFDIPVIGITGSNGKTI 120

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 112 LKENLKYFISQTSTVSVSIKSYNNYLGVLISLININLSSSFAIFEIGTNNFEEIKNLTSIIMPSQAIITNILPTHLENFK 191
Cdd:PRK11930  121 VKEWLYQLLSPDYNIVRSPRSYNSQIGVPLSVWQLNEEHELGIFEAGISQPGEMEALQKIIKPTIGILTNIGGAHQENFR 200

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 192 NTRNIAIEKSDIFSpeynpNIELLVLSQNNTDETyiSDLALKENISNIITFGKTNNQ-NYRIEKINKIDDKTfEILICNN 270
Cdd:PRK11930  201 SIKQKIMEKLKLFK-----DCDVIIYNGDNELIS--SCITKSNLTLKLISWSRKDPEaPLYIPFVEKKEDHT-VISYTYK 272

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 271 TTTYKFEINKNQLSRINNILISLIIFVYNKLDITTFISSTKNVELVEGRgLESKIFINNKIinFIDETYNASPSTMRMCI 350
Cdd:PRK11930  273 GEDFHFEIPFIDDASIENLIHCIAVLLYLGYSADQIQERMARLEPVAMR-LEVKEGINNCT--LINDSYNSDLQSLDIAL 349

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 351 DEFSNIKtmKNQKKILILGDMKELGEEALGFHLEILEYIITLDLNNVIICGELMKSalNKLSKKDLKIELMLNEKLIIKY 430
Cdd:PRK11930  350 DFLNRRS--QSKKKTLILSDILQSGQSPEELYRKVAQLISKRGIDRLIGIGEEISS--EASKFEGTEKEFFKTTEAFLKS 425

                         410
                  ....*....|....*.
gi 1347645565 431 LKKTLNNNDILLIKGS 446
Cdd:PRK11930  426 FAFLKFRNELILVKGA 441
murF PRK10773
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; Reviewed
 34-205 1.49e-16

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; Reviewed


Pssm-ID: 182718 [Multi-domain]  Cd Length: 453  Bit Score: 81.62  E-value: 1.49e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  34 VFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFifnKILVVDDILTFLLSTAKIKRNIFKGKVIGITGSVGKTTLK 113
Cdd:PRK10773   39 LFVALKGERFDAHDFADDAKAAGAGALLVSRPLDIDL---PQLVVKDTRLAFGQLAAWVRQQVPARVVALTGSSGKTSVK 115

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 114 ENLKYFISQTSTVSVSIKSYNNYLGVLISLININLSSSFAIFEIGTNNFEEIKNLTSIIMPSQAIITNILPTHLENFKNT 193
Cdd:PRK10773  116 EMTAAILRQCGNTLYTAGNLNNDIGVPLTLLRLTPEHDYAVIELGANHQGEIAYTVSLTRPEAALVNNLAAAHLEGFGSL 195

                         170
                  ....*....|..
gi 1347645565 194 RNIAIEKSDIFS 205
Cdd:PRK10773  196 AGVAKAKGEIFS 207
Mur_ligase_M pfam08245
Mur ligase middle domain;
103-256 2.07e-13

Mur ligase middle domain;


Pssm-ID: 462409 [Multi-domain]  Cd Length: 199  Bit Score: 68.87  E-value: 2.07e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 103 ITGSVGKTTLKEN----LKYFISQTSTV----SVSIKSYNNYLGVLISLINI-NLSSSFAIFEIGTNNFEEiKNLTSIIM 173
Cdd:pfam08245   1 VTGTNGKTTTTELiaaiLSLAGGVIGTIgtyiGKSGNTTNNAIGLPLTLAEMvEAGAEYAVLEVSSHGLGE-GRLSGLLK 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 174 PSQAIITNILPTHLENFKNTRNIAIEKSDIFSPEYNPNIelLVLsqnNTDETYISDLA--LKENISNIITFGKTNNQNYR 251
Cdd:pfam08245  80 PDIAVFTNISPDHLDFHGTMENYAKAKAELFEGLPEDGI--AVI---NADDPYGAFLIakLKKAGVRVITYGIEGEADLR 154


                  ....*
gi 1347645565 252 IEKIN 256
Cdd:pfam08245 155 AANIE 159
murE TIGR01085
UDP-N-acetylmuramyl-tripeptide synthetase; Most members of this family are EC 6.3.2.13, ...
 25-111 1.08e-11

UDP-N-acetylmuramyl-tripeptide synthetase; Most members of this family are EC 6.3.2.13, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase. An exception is Staphylococcus aureus, in which diaminopimelate is replaced by lysine in the peptidoglycan and MurE is EC 6.3.2.7. The Mycobacteria, part of the closest neighboring branch outside of the low-GC Gram-positive bacteria, use diaminopimelate. A close homolog, scoring just below the trusted cutoff, is found (with introns) in Arabidopsis thaliana. Its role is unknown. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273435 [Multi-domain]  Cd Length: 464  Bit Score: 66.57  E-value: 1.08e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  25 SSLEINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFIFNKILVVDDILTFLLSTAKI-----KRNIfkgK 99
Cdd:TIGR01085  10 DSREVKPGDLFVAIKGTHVDGHDFIHDAIANGAVAVVVERDVDFYVAPVPVIIVPDLRHALSSLAAAfyghpSKKL---K 86

                          90
                  ....*....|..
gi 1347645565 100 VIGITGSVGKTT 111
Cdd:TIGR01085  87 VIGVTGTNGKTT 98
MurE COG0769
UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 28-111 1.81e-11

UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl tripeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440532 [Multi-domain]  Cd Length: 459  Bit Score: 65.87  E-value: 1.81e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  28 EINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFIFNKILVVDDILTFLlstAKIKRNIF-----KGKVIG 102
Cdd:COG0769     8 KVKPGDLFVALPGARVDGHDFIAQAIARGAVAVVTEAPGALLAAGVPVIVVPDPRAAL---ALLAAAFYghpsqKLKLIG 84


                  ....*....
gi 1347645565 103 ITGSVGKTT 111
Cdd:COG0769    85 VTGTNGKTT 93
PRK11929 PRK11929
bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE ...
 26-111 1.66e-09

bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF;


Pssm-ID: 237025 [Multi-domain]  Cd Length: 958  Bit Score: 60.10  E-value: 1.66e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  26 SLEINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDRKLEDNFIFNKILV--VDDILTFLlstAKIKRNiFKGK---- 99
Cdd:PRK11929   36 SREVQPGDLFVACRGAASDGRAFIDQALARGAAAVLVEAEGEDQVAAADALVlpVADLRKAL---GELAAR-WYGRpseq 111

                          90
                  ....*....|....
gi 1347645565 100 --VIGITGSVGKTT 111
Cdd:PRK11929  112 lsLVAVTGTNGKTS 125
murE PRK00139
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional
 26-111 5.44e-09

UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional


Pssm-ID: 234660 [Multi-domain]  Cd Length: 460  Bit Score: 58.22  E-value: 5.44e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  26 SLEINKDDVFISLLGNNTHGNQYIPDAINKGARYIITDrklEDNFIFNK--ILVVDDILTFLlstAKIKRNIF-----KG 98
Cdd:PRK00139   22 SRKVKPGDLFVALPGHKVDGRDFIAQAIANGAAAVVAE---ADGEAGTGvpVIIVPDLRKAL---ALLAAAFYghpsdKL 95

                          90
                  ....*....|...
gi 1347645565  99 KVIGITGSVGKTT 111
Cdd:PRK00139   96 KLIGVTGTNGKTT 108
murD TIGR01087
UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation ...
 95-253 4.22e-04

UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273436 [Multi-domain]  Cd Length: 433  Bit Score: 42.71  E-value: 4.22e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565  95 IFKGKVIGITGSVGKTT--------LKEN-LKYFISQTSTVSVSIKSYNNYLGVLISLIninlsSSFAIFeiGTNNFEei 165
Cdd:TIGR01087  99 LVPLPVVAITGTNGKTTttsllyhlLKAAgLKAFLGGNIGTPALEVLDQEGAELYVLEL-----SSFQLE--TTESLR-- 169

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1347645565 166 knltsiimPSQAIITNILPTHLENFKNTRNIAIEKSDIFspEYNPNIELLVLsqNNTDETYISdlALKENISNIITFGKT 245
Cdd:TIGR01087 170 --------PEIALILNISEDHLDWHGSFEDYVAAKLKIF--ARQTEGDVAVL--NADDPRFAR--LAQKSKAQVIWFSVE 235


                  ....*...
gi 1347645565 246 NNQNYRIE 253
Cdd:TIGR01087 236 KDAERGLC 243
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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