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Conserved domains on  [gi|1339549013|gb|AUZ17333|]
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nourseothricin resistance protein [Cloning vector pCA-DEST2300]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10456837)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  15581578|10940244|9175471|27367672|26818562
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 39-140 8.90e-18

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


:

Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 74.48  E-value: 8.90e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  39 TRSVSPYRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDL--ASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQ 116
Cdd:pfam00583  13 PEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEPpvGEIEGLAVAPEYRGKGIGTALLQALLEWARERG 92

                          90       100
                  ....*....|....*....|....
gi 1339549013 117 LLGIRLETQTNNVPACNLYAKCGF 140
Cdd:pfam00583  93 CERIFLEVAADNLAAIALYEKLGF 116
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 39-140 8.90e-18

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 74.48  E-value: 8.90e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  39 TRSVSPYRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDL--ASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQ 116
Cdd:pfam00583  13 PEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEPpvGEIEGLAVAPEYRGKGIGTALLQALLEWARERG 92

                          90       100
                  ....*....|....*....|....
gi 1339549013 117 LLGIRLETQTNNVPACNLYAKCGF 140
Cdd:pfam00583  93 CERIFLEVAADNLAAIALYEKLGF 116
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 82-141 4.37e-15

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 66.99  E-value: 4.37e-15
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  82 DLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFT 141
Cdd:COG0456    12 DEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFE 71
PRK10975 PRK10975
dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;
60-142 1.11e-08

dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;


Pssm-ID: 182877  Cd Length: 194  Bit Score: 52.24  E-value: 1.11e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  60 ACYGAFIDQELV-----GKI-------ELNSTwndLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTN 127
Cdd:PRK10975   94 AVRGTFDHQCLLlrdasGQIqgfvtlrELNDT---DARIGLLAVFPGAQGRGIGARLMQAALNWCQARGLTRLRVATQMG 170

                          90
                  ....*....|....*
gi 1339549013 128 NVPACNLYAKCGFTL 142
Cdd:PRK10975  171 NLAALRLYIRSGANI 185
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 61-123 2.71e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 48.43  E-value: 2.71e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1339549013  61 CYGAFIDQELVGKIELNSTW--NDLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLE 123
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGsgGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 39-140 8.90e-18

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 74.48  E-value: 8.90e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  39 TRSVSPYRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDL--ASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQ 116
Cdd:pfam00583  13 PEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEPpvGEIEGLAVAPEYRGKGIGTALLQALLEWARERG 92

                          90       100
                  ....*....|....*....|....
gi 1339549013 117 LLGIRLETQTNNVPACNLYAKCGF 140
Cdd:pfam00583  93 CERIFLEVAADNLAAIALYEKLGF 116
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 82-141 4.37e-15

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 66.99  E-value: 4.37e-15
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  82 DLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFT 141
Cdd:COG0456    12 DEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFE 71
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 61-141 1.31e-11

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 57.46  E-value: 1.31e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  61 CYGAFIDQELVGKIELNSTWNDLASIE-HIVVSHTHRGKGVAHSLIEFAKKWAlsrQLLGIRLETQTNNVPACNLYAKCG 139
Cdd:pfam13508   5 FFVAEDDGKIVGFAALLPLDDEGALAElRLAVHPEYRGQGIGRALLEAAEAAA---KEGGIKLLELETTNRAAAFYEKLG 81


                  ..
gi 1339549013 140 FT 141
Cdd:pfam13508  82 FE 83
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 28-141 2.42e-11

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 58.14  E-value: 2.42e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  28 VHLSDQGFELSTRSVSPYRKDYISDDDSDedsACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSLIEF 107
Cdd:COG0454     6 ATPEDINFILLIEALDAELKAMEGSLAGA---EFIAVDDKGEPIGFAGLRRLDDKVLELKRLYVLPEYRGKGIGKALLEA 82

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1339549013 108 AKKWALSRQLLGIRLETQTNNVPACNLYAKCGFT 141
Cdd:COG0454    83 LLEWARERGCTALELDTLDGNPAAIRFYERLGFK 116
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 61-162 2.75e-11

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 58.08  E-value: 2.75e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  61 CYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETqtnNVPACNLYAKCGF 140
Cdd:COG1246    30 FWVAEEDGEIVGCAALHPLDEDLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLT---TSAAIHFYEKLGF 106

                          90       100
                  ....*....|....*....|..
gi 1339549013 141 TLGGIDLFTYKtRPQVSNETAM 162
Cdd:COG1246   107 EEIDKEDLPYA-KVWQRDSVVM 127
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
69-144 3.22e-10

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 53.76  E-value: 3.22e-10
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1339549013  69 ELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGG 144
Cdd:COG3393     1 ELVAMAGVRAESPGVAEISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAARRLYERLGFRPVG 76
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
62-141 1.61e-09

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 53.26  E-value: 1.61e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  62 YGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPacnLYAKCGFT 141
Cdd:COG2153    37 LLAYDDGELVATARLLPPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRIVLSAQAHAVG---FYEKLGFV 113
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
61-141 9.29e-09

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 51.62  E-value: 9.29e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  61 CYGAFIDQELVG-----KIELNSTWNDLAsIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPacnLY 135
Cdd:COG3153    41 SLVAEDDGEIVGhvalsPVDIDGEGPALL-LGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLP---FY 116


                  ....*.
gi 1339549013 136 AKCGFT 141
Cdd:COG3153   117 ERFGFR 122
PRK10975 PRK10975
dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;
60-142 1.11e-08

dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;


Pssm-ID: 182877  Cd Length: 194  Bit Score: 52.24  E-value: 1.11e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  60 ACYGAFIDQELV-----GKI-------ELNSTwndLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTN 127
Cdd:PRK10975   94 AVRGTFDHQCLLlrdasGQIqgfvtlrELNDT---DARIGLLAVFPGAQGRGIGARLMQAALNWCQARGLTRLRVATQMG 170

                          90
                  ....*....|....*
gi 1339549013 128 NVPACNLYAKCGFTL 142
Cdd:PRK10975  171 NLAALRLYIRSGANI 185
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 61-123 2.71e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 48.43  E-value: 2.71e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1339549013  61 CYGAFIDQELVGKIELNSTW--NDLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLE 123
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGsgGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 62-140 1.44e-07

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 48.04  E-value: 1.44e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  62 YGAFIDQELVGKIELNSTWNdlasIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLE--TQTNNVPacnLYAKCG 139
Cdd:pfam13673  34 FVAFEGGQIVGVIALRDRGH----ISLLFVDPDYQGQGIGKALLEAVEDYAEKDGIKLSELTvnASPYAVP---FYEKLG 106


                  .
gi 1339549013 140 F 140
Cdd:pfam13673 107 F 107
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
64-144 2.49e-07

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 48.07  E-value: 2.49e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  64 AFIDQELVG--KIELNSTWNDLASI--EHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCG 139
Cdd:COG1247    57 AEEDGEVVGfaSLGPFRPRPAYRGTaeESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLG 136


                  ....*
gi 1339549013 140 FTLGG 144
Cdd:COG1247   137 FEEVG 141
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 67-142 1.36e-06

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 46.15  E-value: 1.36e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339549013  67 DQELVGKIELNSTWNDLASIE-HIVVSHTHRGKGVAHSLIEFAKKWALSRqlLG---IRLETQTNNVPACNLYAKCGFTL 142
Cdd:COG1670    70 DGELIGVVGLYDIDRANRSAEiGYWLAPAYWGKGYATEALRALLDYAFEE--LGlhrVEAEVDPDNTASIRVLEKLGFRL 147
PRK07757 PRK07757
N-acetyltransferase;
64-140 4.54e-04

N-acetyltransferase;


Pssm-ID: 236088 [Multi-domain]  Cd Length: 152  Bit Score: 38.64  E-value: 4.54e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1339549013  64 AFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSrqlLGI-RLETQTNNVpacNLYAKCGF 140
Cdd:PRK07757   46 AEEEGEIVGCCALHILWEDLAEIRSLAVSEDYRGQGIGRMLVEACLEEARE---LGVkRVFALTYQP---EFFEKLGF 117
PLN02706 PLN02706
glucosamine 6-phosphate N-acetyltransferase
 86-150 1.08e-03

glucosamine 6-phosphate N-acetyltransferase


Pssm-ID: 178308 [Multi-domain]  Cd Length: 150  Bit Score: 37.76  E-value: 1.08e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1339549013  86 IEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPacnLYAKCGFTLGGIDLFTY 150
Cdd:PLN02706   88 IEDVVVDSAARGKGLGKKIIEALTEHARSAGCYKVILDCSEENKA---FYEKCGYVRKEIQMVKY 149
PRK07922 PRK07922
amino-acid N-acetyltransferase;
67-115 2.54e-03

amino-acid N-acetyltransferase;


Pssm-ID: 236132 [Multi-domain]  Cd Length: 169  Bit Score: 36.82  E-value: 2.54e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1339549013  67 DQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH----SLIEFAKKWALSR 115
Cdd:PRK07922   54 DGEVVGCGALHVMWEDLAEIRTVAVDPAARGRGVGHaiveRLLDVARELGLSR 106
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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