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Conserved domains on  [gi|1308557238|gb|PKL68532|]
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N-acetyltransferase [Methanomicrobiales archaeon HGW-Methanomicrobiales-1]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10003213)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 4-127 5.88e-13

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


:

Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 61.93  E-value: 5.88e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238   4 VNVRELSPAELVRAeRELWTHYHnqtADRNNDRLFAAFAGTKLIGVARCSRHPDGL-EVDAVYVLDEYRRRGFARSVMIL 82
Cdd:COG1246     1 MTIRPATPDDVPAI-LELIRPYA---LEEEIGEFWVAEEDGEIVGCAALHPLDEDLaELRSLAVHPDYRGRGIGRRLLEA 76

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1308557238  83 LIEECGRQ--ETLFMHSKTELVDFYGSLGFYPIAEIDLPKTIRDRFD 127
Cdd:COG1246    77 LLAEARELglKRLFLLTTSAAIHFYEKLGFEEIDKEDLPYAKVWQRD 123
 
Name Accession Description Interval E-value
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 4-127 5.88e-13

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 61.93  E-value: 5.88e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238   4 VNVRELSPAELVRAeRELWTHYHnqtADRNNDRLFAAFAGTKLIGVARCSRHPDGL-EVDAVYVLDEYRRRGFARSVMIL 82
Cdd:COG1246     1 MTIRPATPDDVPAI-LELIRPYA---LEEEIGEFWVAEEDGEIVGCAALHPLDEDLaELRSLAVHPDYRGRGIGRRLLEA 76

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1308557238  83 LIEECGRQ--ETLFMHSKTELVDFYGSLGFYPIAEIDLPKTIRDRFD 127
Cdd:COG1246    77 LLAEARELglKRLFLLTTSAAIHFYEKLGFEEIDKEDLPYAKVWQRD 123
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 20-143 2.54e-10

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 54.97  E-value: 2.54e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238  20 ELWTHYHNQTA-----DRNNDRLFAAFAGTKLIGVA--RCSRHpdgleVDAVYVLDEYRRRGFARSVMILLIEECGRQE- 91
Cdd:pfam13673  11 ETFYEFISPEAlreriDQGEYFFFVAFEGGQIVGVIalRDRGH-----ISLLFVDPDYQGQGIGKALLEAVEDYAEKDGi 85

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1308557238  92 ---TLFMHSKTELVDFYGSLGFYPIAEidlpktirdrfdfcMGNLKGIDVCPMKR 143
Cdd:pfam13673  86 klsELTVNASPYAVPFYEKLGFRATGP--------------EQEFNGIRFVPMEK 126
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 37-90 5.89e-06

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 41.88  E-value: 5.89e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1308557238  37 LFAAFAGTKLIGVARCSRH---PDGLEVDAVYVLDEYRRRGFARSVMILLIEECGRQ 90
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDgsgGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARER 57
 
Name Accession Description Interval E-value
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 4-127 5.88e-13

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 61.93  E-value: 5.88e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238   4 VNVRELSPAELVRAeRELWTHYHnqtADRNNDRLFAAFAGTKLIGVARCSRHPDGL-EVDAVYVLDEYRRRGFARSVMIL 82
Cdd:COG1246     1 MTIRPATPDDVPAI-LELIRPYA---LEEEIGEFWVAEEDGEIVGCAALHPLDEDLaELRSLAVHPDYRGRGIGRRLLEA 76

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1308557238  83 LIEECGRQ--ETLFMHSKTELVDFYGSLGFYPIAEIDLPKTIRDRFD 127
Cdd:COG1246    77 LLAEARELglKRLFLLTTSAAIHFYEKLGFEEIDKEDLPYAKVWQRD 123
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
30-126 2.19e-10

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 55.48  E-value: 2.19e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238  30 ADRNNDRLFAAFAGTKLIGVARCSR-----HPDGLEVDAVYVLDEYRRRGFARSVMILLIEECGRQ--ETLFMHSKTELV 102
Cdd:COG3153    34 EDPAAGLSLVAEDDGEIVGHVALSPvdidgEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERgaRAVVLLGDPSLL 113

                          90       100
                  ....*....|....*....|....
gi 1308557238 103 DFYGSLGFYPIAEIDLPKTIRDRF 126
Cdd:COG3153   114 PFYERFGFRPAGELGLTLGPDEVF 137
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 20-143 2.54e-10

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 54.97  E-value: 2.54e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238  20 ELWTHYHNQTA-----DRNNDRLFAAFAGTKLIGVA--RCSRHpdgleVDAVYVLDEYRRRGFARSVMILLIEECGRQE- 91
Cdd:pfam13673  11 ETFYEFISPEAlreriDQGEYFFFVAFEGGQIVGVIalRDRGH-----ISLLFVDPDYQGQGIGKALLEAVEDYAEKDGi 85

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1308557238  92 ---TLFMHSKTELVDFYGSLGFYPIAEidlpktirdrfdfcMGNLKGIDVCPMKR 143
Cdd:pfam13673  86 klsELTVNASPYAVPFYEKLGFRATGP--------------EQEFNGIRFVPMEK 126
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 6-110 1.15e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 52.91  E-value: 1.15e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238   6 VRELSPAELVRAERELWTHYHNQTADRNNDRLFAAFAGTKLIGVARCSRHPDG---LEVDAVYVLDEYRRRGFARSVMIL 82
Cdd:pfam00583   4 LYELLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEppvGEIEGLAVAPEYRGKGIGTALLQA 83

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1308557238  83 LIEECGRQETLFMHSKTEL-----VDFYGSLGF 110
Cdd:pfam00583  84 LLEWARERGCERIFLEVAAdnlaaIALYEKLGF 116
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
45-116 2.42e-08

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 48.37  E-value: 2.42e-08
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1308557238  45 KLIGVARCSRHPDGL-EVDAVYVLDEYRRRGFARSVMILLIEECGRQ--ETLFMHSK---TELVDFYGSLGFYPIAEI 116
Cdd:COG3393     1 ELVAMAGVRAESPGVaEISGVYTHPEYRGRGLASALVAALAREALARgaRTPFLYVDadnPAARRLYERLGFRPVGEY 78
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 34-116 5.05e-08

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 48.90  E-value: 5.05e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238  34 NDRLFAAFAGTKLIGVARCSRHPD-GLEVDAVYVLDEYRRRGFARSVMILLIEECGRQETLFMHSKT-----ELVDFYGS 107
Cdd:COG0454    33 GAEFIAVDDKGEPIGFAGLRRLDDkVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTldgnpAAIRFYER 112


                  ....*....
gi 1308557238 108 LGFYPIAEI 116
Cdd:COG0454   113 LGFKEIERY 121
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 33-110 9.29e-08

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 47.06  E-value: 9.29e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238  33 NNDRLFAAFAGTKLIGVARCSRHPDG--LEVDAVYVLDEYRRRGFARSVM--ILLIEECGRQETLFMHSKTELVDFYGSL 108
Cdd:pfam13508   1 PGGRFFVAEDDGKIVGFAALLPLDDEgaLAELRLAVHPEYRGQGIGRALLeaAEAAAKEGGIKLLELETTNRAAAFYEKL 80


                  ..
gi 1308557238 109 GF 110
Cdd:pfam13508  81 GF 82
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
38-116 1.11e-07

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 47.87  E-value: 1.11e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238  38 FAAFAGTKLIGVARCsRHPDGLEV--DAVYVLDEYRRRGFARSVMILLIEECGRQ--ETLFMHSKTELVDFYGSLGFYPI 113
Cdd:COG2153    37 LLAYDDGELVATARL-LPPGDGEAkiGRVAVLPEYRGQGLGRALMEAAIEEARERgaRRIVLSAQAHAVGFYEKLGFVPV 115


                  ...
gi 1308557238 114 AEI 116
Cdd:COG2153   116 GEE 118
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 37-90 5.89e-06

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 41.88  E-value: 5.89e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1308557238  37 LFAAFAGTKLIGVARCSRH---PDGLEVDAVYVLDEYRRRGFARSVMILLIEECGRQ 90
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDgsgGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARER 57
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 48-117 7.40e-05

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 39.25  E-value: 7.40e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1308557238  48 GVARCSRHPDG--LEVDAVYVLDEYRRRGFARSVMILLIEECGRQ--ETLFMHSKTE---LVDFYGSLGFYPIAEID 117
Cdd:COG0456     1 GFALLGLVDGGdeAEIEDLAVDPEYRGRGIGRALLEAALERARERgaRRLRLEVREDneaAIALYEKLGFEEVGERP 77
FR47 pfam08445
FR47-like protein; The members of this family are similar to the C-terminal region of the D. ...
 55-110 2.87e-04

FR47-like protein; The members of this family are similar to the C-terminal region of the D. melanogaster hypothetical protein FR47. This protein has been found to consist of two N-acyltransferase-like domains swapped with the C-terminal strands.


Pssm-ID: 117022 [Multi-domain]  Cd Length: 86  Bit Score: 37.69  E-value: 2.87e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238  55 HPDGLEVDAVYVLDEYRRRGFARSVMILLIEECG-RQETLFMH---SKTELVDFYGSLGF 110
Cdd:pfam08445  18 RLPGGELGALQTLPEHRRRGLGSRLVAALARGIAeRGITPFAVvvaGNTPSRRLYEKLGF 77
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
17-117 1.23e-03

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 37.28  E-value: 1.23e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1308557238  17 AERELWTHYHNQtadrNNDRLFAAFAGTKLIGVARCSRH-----PDGLEVDAVYVLDEYRRRGFARSVMILLIEECGRQE 91
Cdd:COG1247    38 EEREAWFAAILA----PGRPVLVAEEDGEVVGFASLGPFrprpaYRGTAEESIYVDPDARGRGIGRALLEALIERARARG 113

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1308557238  92 TLFMHSKTEL-----VDFYGSLGFYPIAEID 117
Cdd:COG1247   114 YRRLVAVVLAdneasIALYEKLGFEEVGTLP 144
Citrate_lyase_ligase cd02169
Citrate lyase ligase; Citrate lyase ligase, also known as [Citrate (pro-3S)-lyase] ligase, is ...
 64-117 2.13e-03

Citrate lyase ligase; Citrate lyase ligase, also known as [Citrate (pro-3S)-lyase] ligase, is responsible for acetylation of the (2-(5''-phosphoribosyl)-3'-dephosphocoenzyme-A) prosthetic group of the gamma subunit of citrate lyase, converting the inactive thiol form of this enzyme to the active form. The acetylation of 1 molecule of deacetyl-citrate lyase to enzymatically active citrate lyase requires 6 molecules of ATP. The Adenylylyltranferase activity of the enzyme involves the formation of AMP and and pyrophosphate in the acetylation reaction.


Pssm-ID: 173920 [Multi-domain]  Cd Length: 297  Bit Score: 37.24  E-value: 2.13e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1308557238  64 VYVLDEYRRRGFARSVMILLIE-ECGRQET-LFMHSKTELVDFYGSLGFYPIAEID 117
Cdd:cd02169    31 VAVCPKYQGEGLALKIVSELINkAYEEGIFhLFLFTKPKNAKFFRGLGFKELANAS 86
GNAT_acetyltran pfam12746
GNAT acetyltransferase; Many of the members are annotated s being Zwittermicin A resistance ...
 49-87 2.50e-03

GNAT acetyltransferase; Many of the members are annotated s being Zwittermicin A resistance proteins, whereas others are listed as being GNAT acetyltransferases. The family has similarities to the GNAT acetyltransferase family.


Pssm-ID: 403833  Cd Length: 239  Bit Score: 36.87  E-value: 2.50e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1308557238  49 VARCS---RHPDGLEVDaVYVLDEYRRRGFARSVMILLIEEC 87
Cdd:pfam12746 165 VSGASsysVYEGGIEIE-IDTHPDYRGKGLATICAAALILEC 205
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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