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Conserved domains on  [gi|1278788493|gb|PJC81094|]
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hypothetical protein CO008_00025 [Candidatus Roizmanbacteria bacterium CG_4_8_14_3_um_filter_36_12]

Protein Classification

glycosyltransferase domain-containing protein( domain architecture ID 1905369)

glycosyltransferase domain-containing protein contains a Rossmann-fold domain reminiscent of glycosyltransferases as found in the multifunctional procollagen lysine hydroxylase and glycosyltransferase LH; may have glycosyltransferase activity

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GT_LH super family cl45890
catalytic glycosyltransferase (GT) domain found in the lysyl hydroxylase (LH) family; The ...
 5-203 1.59e-05

catalytic glycosyltransferase (GT) domain found in the lysyl hydroxylase (LH) family; The lysyl hydroxylase (LH) family includes LH1-3. LH1 (EC 1.14.11.4, also called procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 or PLOD1) and LH2 (EC 1.14.11.4, also called procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 or PLOD2) form hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines may serve as sites of attachment for carbohydrate units and are essential for stability of the intermolecular collagen cross-links. LH1 is part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linking of collagen fibrils. LH3 (EC 1.14.11.4/EC 2.4.1.50/EC 2.4.1.66), also called procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 (PLOD3), or multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, is a multifunctional enzyme that catalyzes a series of essential post-translational modifications on Lys residues in procollagen. It converts collagen lysines into 1,2-glucosylgalactosyl-5-hydroxylysines through three consecutive reactions: hydroxylation of collagen lysines (LH activity), N-linked conjugation of galactose to hydroxylysines (GT activity), and conjugation of glucose to galactosyl-5-hydroxylysines (GGT activity). LH3 monomer encompasses three domains; the first two N-terminal domains (catalytic glycosyltransferase domain and accessory domain) show Rossmann-fold architectures reminiscent of glycosyltransferases, whereas the C-terminal domain is characterized by a double-stranded beta-helix (DSBH) fold, highly conserved among the 2-oxoglutarate, Fe2+-dependent dioxygenases. This model corresponds to the N-terminal catalytic glycosyltransferase (GT) domain, which is solely responsible for glycosyltransferase activities (both GT and GGT).


The actual alignment was detected with superfamily member cd22997:

Pssm-ID: 459235  Cd Length: 247  Bit Score: 45.08  E-value: 1.59e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1278788493   5 VIITCANSKHgdfviDHWFKSLKDNIPLSNIDVVIIDYGLTYSQKNKLKKEKVILFKgtkkyhivnkrffdacRYLKTKK 84
Cdd:cd22997     1 HVLTPATKPN-----DGLCRTLLSAALLGYPPPTVLGWGEKWKGGDGGHGAKIRLLL----------------EYLESLK 59

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1278788493  85 YDQ---VLFVDGGDIIFQEDITPV----FKKNKNTF--RVV--------PLGMEVLFFEWFIFNNFADGVKEKIWKVVKN 147
Cdd:cd22997    60 LPDddlVLFVDGYDVWFQLPPEELlerfLALNAAAIrqPIVfsaekncwPDDSLAPAYPAVPPSPLPPDIDDDLDSSKTR 139

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1278788493 148 KPVINAGVIFAPYKKFISLCELMNKLIKDKDAWGPDQIVVNYYiyqskykFLDNKY 203
Cdd:cd22997   140 PRYLNSGGIIGRAGDLRELLEAALELIEDEKDWDDDQLVFTEL-------YLEQEY 188
 
Name Accession Description Interval E-value
GT_LH cd22997
catalytic glycosyltransferase (GT) domain found in the lysyl hydroxylase (LH) family; The ...
 5-203 1.59e-05

catalytic glycosyltransferase (GT) domain found in the lysyl hydroxylase (LH) family; The lysyl hydroxylase (LH) family includes LH1-3. LH1 (EC 1.14.11.4, also called procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 or PLOD1) and LH2 (EC 1.14.11.4, also called procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 or PLOD2) form hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines may serve as sites of attachment for carbohydrate units and are essential for stability of the intermolecular collagen cross-links. LH1 is part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linking of collagen fibrils. LH3 (EC 1.14.11.4/EC 2.4.1.50/EC 2.4.1.66), also called procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 (PLOD3), or multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, is a multifunctional enzyme that catalyzes a series of essential post-translational modifications on Lys residues in procollagen. It converts collagen lysines into 1,2-glucosylgalactosyl-5-hydroxylysines through three consecutive reactions: hydroxylation of collagen lysines (LH activity), N-linked conjugation of galactose to hydroxylysines (GT activity), and conjugation of glucose to galactosyl-5-hydroxylysines (GGT activity). LH3 monomer encompasses three domains; the first two N-terminal domains (catalytic glycosyltransferase domain and accessory domain) show Rossmann-fold architectures reminiscent of glycosyltransferases, whereas the C-terminal domain is characterized by a double-stranded beta-helix (DSBH) fold, highly conserved among the 2-oxoglutarate, Fe2+-dependent dioxygenases. This model corresponds to the N-terminal catalytic glycosyltransferase (GT) domain, which is solely responsible for glycosyltransferase activities (both GT and GGT).


Pssm-ID: 438557  Cd Length: 247  Bit Score: 45.08  E-value: 1.59e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1278788493   5 VIITCANSKHgdfviDHWFKSLKDNIPLSNIDVVIIDYGLTYSQKNKLKKEKVILFKgtkkyhivnkrffdacRYLKTKK 84
Cdd:cd22997     1 HVLTPATKPN-----DGLCRTLLSAALLGYPPPTVLGWGEKWKGGDGGHGAKIRLLL----------------EYLESLK 59

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1278788493  85 YDQ---VLFVDGGDIIFQEDITPV----FKKNKNTF--RVV--------PLGMEVLFFEWFIFNNFADGVKEKIWKVVKN 147
Cdd:cd22997    60 LPDddlVLFVDGYDVWFQLPPEELlerfLALNAAAIrqPIVfsaekncwPDDSLAPAYPAVPPSPLPPDIDDDLDSSKTR 139

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1278788493 148 KPVINAGVIFAPYKKFISLCELMNKLIKDKDAWGPDQIVVNYYiyqskykFLDNKY 203
Cdd:cd22997   140 PRYLNSGGIIGRAGDLRELLEAALELIEDEKDWDDDQLVFTEL-------YLEQEY 188
Glyco_transf_8 pfam01501
Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to ...
 46-238 1.27e-03

Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.


Pssm-ID: 279798 [Multi-domain]  Cd Length: 252  Bit Score: 39.23  E-value: 1.27e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1278788493  46 YSQKNKLKKEKVILFKGTKKY--HIVNKRFFDAcRYLKtkKYDQVLFVDGgDIIFQEDITPVF--KKNKNTFRVVPLGME 121
Cdd:pfam01501  61 LESDIKIFEYFSKLKLRSPKYwsLLNYLRLYLP-DLFP--KLDKILYLDA-DIVVQGDLSPLWdiDLGGKVLAAVEDNYF 136

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1278788493 122 VLFFE---WFIFNNFadGVKEKIWkvvknkpviNAGVIFA-----PYKKFISLC-ELMNKLIKDKDAWGPDQIVVNYYIY 192
Cdd:pfam01501 137 QRYPNfsePIILENF--GPPACYF---------NAGMLLFdldawRKENITERYiKWLNLNENRTLWKLGDQDPLNIVFY 205

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1278788493 193 QsKYKFLDNKYNYMMSTMPkgfiiKSGVFYEPSGEKIAIVHNAGQM 238
Cdd:pfam01501 206 G-KVKPLDPRWNVLGLGYY-----NKKKSLNEITENAAVIHYNGPT 245
 
Name Accession Description Interval E-value
GT_LH cd22997
catalytic glycosyltransferase (GT) domain found in the lysyl hydroxylase (LH) family; The ...
 5-203 1.59e-05

catalytic glycosyltransferase (GT) domain found in the lysyl hydroxylase (LH) family; The lysyl hydroxylase (LH) family includes LH1-3. LH1 (EC 1.14.11.4, also called procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 or PLOD1) and LH2 (EC 1.14.11.4, also called procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 or PLOD2) form hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines may serve as sites of attachment for carbohydrate units and are essential for stability of the intermolecular collagen cross-links. LH1 is part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linking of collagen fibrils. LH3 (EC 1.14.11.4/EC 2.4.1.50/EC 2.4.1.66), also called procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 (PLOD3), or multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, is a multifunctional enzyme that catalyzes a series of essential post-translational modifications on Lys residues in procollagen. It converts collagen lysines into 1,2-glucosylgalactosyl-5-hydroxylysines through three consecutive reactions: hydroxylation of collagen lysines (LH activity), N-linked conjugation of galactose to hydroxylysines (GT activity), and conjugation of glucose to galactosyl-5-hydroxylysines (GGT activity). LH3 monomer encompasses three domains; the first two N-terminal domains (catalytic glycosyltransferase domain and accessory domain) show Rossmann-fold architectures reminiscent of glycosyltransferases, whereas the C-terminal domain is characterized by a double-stranded beta-helix (DSBH) fold, highly conserved among the 2-oxoglutarate, Fe2+-dependent dioxygenases. This model corresponds to the N-terminal catalytic glycosyltransferase (GT) domain, which is solely responsible for glycosyltransferase activities (both GT and GGT).


Pssm-ID: 438557  Cd Length: 247  Bit Score: 45.08  E-value: 1.59e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1278788493   5 VIITCANSKHgdfviDHWFKSLKDNIPLSNIDVVIIDYGLTYSQKNKLKKEKVILFKgtkkyhivnkrffdacRYLKTKK 84
Cdd:cd22997     1 HVLTPATKPN-----DGLCRTLLSAALLGYPPPTVLGWGEKWKGGDGGHGAKIRLLL----------------EYLESLK 59

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1278788493  85 YDQ---VLFVDGGDIIFQEDITPV----FKKNKNTF--RVV--------PLGMEVLFFEWFIFNNFADGVKEKIWKVVKN 147
Cdd:cd22997    60 LPDddlVLFVDGYDVWFQLPPEELlerfLALNAAAIrqPIVfsaekncwPDDSLAPAYPAVPPSPLPPDIDDDLDSSKTR 139

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1278788493 148 KPVINAGVIFAPYKKFISLCELMNKLIKDKDAWGPDQIVVNYYiyqskykFLDNKY 203
Cdd:cd22997   140 PRYLNSGGIIGRAGDLRELLEAALELIEDEKDWDDDQLVFTEL-------YLEQEY 188
Glyco_transf_8 pfam01501
Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to ...
 46-238 1.27e-03

Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.


Pssm-ID: 279798 [Multi-domain]  Cd Length: 252  Bit Score: 39.23  E-value: 1.27e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1278788493  46 YSQKNKLKKEKVILFKGTKKY--HIVNKRFFDAcRYLKtkKYDQVLFVDGgDIIFQEDITPVF--KKNKNTFRVVPLGME 121
Cdd:pfam01501  61 LESDIKIFEYFSKLKLRSPKYwsLLNYLRLYLP-DLFP--KLDKILYLDA-DIVVQGDLSPLWdiDLGGKVLAAVEDNYF 136

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1278788493 122 VLFFE---WFIFNNFadGVKEKIWkvvknkpviNAGVIFA-----PYKKFISLC-ELMNKLIKDKDAWGPDQIVVNYYIY 192
Cdd:pfam01501 137 QRYPNfsePIILENF--GPPACYF---------NAGMLLFdldawRKENITERYiKWLNLNENRTLWKLGDQDPLNIVFY 205

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1278788493 193 QsKYKFLDNKYNYMMSTMPkgfiiKSGVFYEPSGEKIAIVHNAGQM 238
Cdd:pfam01501 206 G-KVKPLDPRWNVLGLGYY-----NKKKSLNEITENAAVIHYNGPT 245
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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