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Conserved domains on  [gi|125575384|gb|EAZ16668|]
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hypothetical protein OsJ_32143 [Oryza sativa Japonica Group]

Protein Classification

MIP/aquaporin family protein( domain architecture ID 239)

MIP (Major Intrinsic Protein)/aquaporin family protein similar to aquaporin that permits osmotically driven movement of water in both directions, and glycerol uptake facilitator protein that mediates glycerol diffusion across the cytoplasmic membrane via a pore-type mechanism

CATH:  1.20.1080.10
Gene Ontology:  GO:0055085|GO:0015267
PubMed:  8849412|11473620|11964478
SCOP:  3000508
TCDB:  1.A.8

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
MIP super family cl00200
Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane ...
 19-309 1.05e-167

Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal cytosolic portion followed by six transmembrane helices, which might have arisen through gene duplication. On the basis of sequence similarity and functional characteristics, the superfamily can be subdivided into two major groups: water-selective channels called aquaporins (AQPs) and glycerol uptake facilitators (GlpFs). AQPs are found in all three kingdoms of life, while GlpFs have been characterized only within microorganisms.


The actual alignment was detected with superfamily member PLN00026:

Pssm-ID: 444743 [Multi-domain]  Cd Length: 298  Bit Score: 467.42  E-value: 1.05e-167
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  19 ASAPATPGTP-APLFAGPRVDSLSY-ERKSMPRCKCLPAaVAEAWAPSaHGCVVEIPAPDVSLTRKLGAEFVGTFILIFF 96
Cdd:PLN00026  10 PSTPATPGTPgGPLFGGFRVDSLSYgDRKLLPSCKCFPV-EALEWGRL-PTCFCDLPPPDVSLTRKLGAEFVGTFILIFA 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  97 ATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQVLGSICAGFAL 176
Cdd:PLN00026  88 ATAGPIVNQKYDGAETLIGNAACAGLAVMIVILSTGHISGAHLNPSLTIAFAALRHFPWKHVPAYIAAQVSASICASFAL 167

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 177 KGVFHPFLSGGVTVpdPTISTAQAFFTEFIITFNLLFVVTAVATDTRAVGELAGIAVGAAVTLNILIAGPTTGGSMNPVR 256
Cdd:PLN00026 168 KGVFHPFMSGGVTV--PSVSTGQAFALEFIITFNLLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVR 245

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|...
gi 125575384 257 TLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAVKLRDENGETPRPQRSFRR 309
Cdd:PLN00026 246 TLGPAVAAGNYRAIWIYLVAPTLGALAGAGTYTAVKLREEDGDPPRPVRSFRR 298
 
Name Accession Description Interval E-value
PLN00026 PLN00026
aquaporin NIP; Provisional
 19-309 1.05e-167

aquaporin NIP; Provisional


Pssm-ID: 177663 [Multi-domain]  Cd Length: 298  Bit Score: 467.42  E-value: 1.05e-167
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  19 ASAPATPGTP-APLFAGPRVDSLSY-ERKSMPRCKCLPAaVAEAWAPSaHGCVVEIPAPDVSLTRKLGAEFVGTFILIFF 96
Cdd:PLN00026  10 PSTPATPGTPgGPLFGGFRVDSLSYgDRKLLPSCKCFPV-EALEWGRL-PTCFCDLPPPDVSLTRKLGAEFVGTFILIFA 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  97 ATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQVLGSICAGFAL 176
Cdd:PLN00026  88 ATAGPIVNQKYDGAETLIGNAACAGLAVMIVILSTGHISGAHLNPSLTIAFAALRHFPWKHVPAYIAAQVSASICASFAL 167

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 177 KGVFHPFLSGGVTVpdPTISTAQAFFTEFIITFNLLFVVTAVATDTRAVGELAGIAVGAAVTLNILIAGPTTGGSMNPVR 256
Cdd:PLN00026 168 KGVFHPFMSGGVTV--PSVSTGQAFALEFIITFNLLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVR 245

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|...
gi 125575384 257 TLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAVKLRDENGETPRPQRSFRR 309
Cdd:PLN00026 246 TLGPAVAAGNYRAIWIYLVAPTLGALAGAGTYTAVKLREEDGDPPRPVRSFRR 298
GlpF COG0580
Glycerol uptake facilitator or related aquaporin (Major Intrinsic protein Family) ...
 78-291 3.88e-60

Glycerol uptake facilitator or related aquaporin (Major Intrinsic protein Family) [Carbohydrate transport and metabolism];


Pssm-ID: 440345 [Multi-domain]  Cd Length: 232  Bit Score: 191.49  E-value: 3.88e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  78 SLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQ 157
Cdd:COG0580    1 SLMKRLLAEFLGTFILVLLGCGVVAVLAGTKGGIGWLGIALAWGLAVLAGVYAFGHISGAHLNPAVTLALAVAGRFPWKD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 158 VPAYVAVQVLGSICAGFALKGVFHP--------FLSGGV--TVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTRAVGE 227
Cdd:COG0580   81 VLPYIIAQLLGAFLGAALVYLIYGGhfdatedgGAKLGVfaTSPAIGYSLGAAFLTEIIGTFVLVFVILGITDKRAAPGG 160

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 228 LAGIAVGAAVTLNILIAGPTTGGSMNPVRTLGPAVAA------GNYRQLWIYLIAPTLGAVAGAGVYTAV 291
Cdd:COG0580  161 LAPLAIGLLVAAIGLSLGPLTGYAINPARDLGPRLFHalgkggSDWGYLWVPIVAPILGAALAALLYRAL 230
MIP cd00333
Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane ...
 81-288 4.50e-53

Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal cytosolic portion followed by six transmembrane helices, which might have arisen through gene duplication. On the basis of sequence similarity and functional characteristics, the superfamily can be subdivided into two major groups: water-selective channels called aquaporins (AQPs) and glycerol uptake facilitators (GlpFs). AQPs are found in all three kingdoms of life, while GlpFs have been characterized only within microorganisms.


Pssm-ID: 238204  Cd Length: 228  Bit Score: 173.21  E-value: 4.50e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  81 RKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACA-GLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVP 159
Cdd:cd00333    1 RKYLAEFLGTFLLVFFGCGSVLAVKLAGGASGGLLGIALAwGFAIFVLVYAVGHISGGHINPAVTLALAVGGRFPLIRVI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 160 AYVAVQVLGSICAGFALKGVF-----------HPFLSGGVTVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTR--AVG 226
Cdd:cd00333   81 PYIIAQLLGAILGAALLYGLYyglyleflganNIVAGIFGTYPSPGVSNGNAFFVEFIGTFILVLVVFATTDDPNgpPPG 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 125575384 227 ELAGIAVGAAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYR---QLWIYLIAPTLGAVAGAGVY 288
Cdd:cd00333  161 GLAPLAIGLLVAAIGLAGGPITGASMNPARSLGPALFTGLARhwhYFWVYWVGPLIGAIAGALVY 225
MIP pfam00230
Major intrinsic protein; MIP (Major Intrinsic Protein) family proteins exhibit essentially two ...
 77-288 1.45e-50

Major intrinsic protein; MIP (Major Intrinsic Protein) family proteins exhibit essentially two distinct types of channel properties: (1) specific water transport by the aquaporins, and (2) small neutral solutes transport, such as glycerol by the glycerol facilitators.


Pssm-ID: 395174 [Multi-domain]  Cd Length: 223  Bit Score: 166.72  E-value: 1.45e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384   77 VSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACA---GLAVTTIILSTGHISGAHLNPSLTIAFAALRHF 153
Cdd:pfam00230   5 VSFLRAVIAEFLATLLFVFFGVGSALGVKKLVSSLAVSGLLAVAlafGLALATLVYCAGHISGAHLNPAVTLALLVGRRF 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  154 PWLQVPAYVAVQVLGSICAGFALKGVFHPFL--SGGVTVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTRAV--GELA 229
Cdd:pfam00230  85 SLLRAIFYIVAQLLGAIAGAALLYGVTVGLQraGLFANTLAPGVNAGQAFVVEIILTFQLVYCVFATTDDRRNGslGTVA 164

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 125575384  230 GIAVGAAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVY 288
Cdd:pfam00230 165 PLAIGFAVFLNHLAGGPYTGASMNPARSFGPAVVLWKWDDHWVYWVGPFIGAALGALVY 223
MIP TIGR00861
MIP family channel proteins; 1.A.8 The Major Intrinsic Protein (MIP) FamilyThe MIP family is ...
 85-288 1.05e-46

MIP family channel proteins; 1.A.8 The Major Intrinsic Protein (MIP) FamilyThe MIP family is large and diverse, possessing over 100 members that all form transmembrane channels. These channel proteins function in water, smallcarbohydrate (e.g., glycerol), urea, NH3, CO2 and possibly ion transport by an energy independent mechanism. They are found ubiquitously in bacteria, archaeaand eukaryotes. The MIP family contains two major groups of channels: aquaporins and glycerol facilitators.The known aquaporins cluster loosely together as do the known glycerol facilitators. MIP family proteins are believed to form aqueous pores that selectively allow passive transport of their solute(s) across the membrane with minimal apparent recognition. Aquaporins selectively transport water (but not glycerol) while glycerol facilitators selectively transport glycerol but not water. Some aquaporins can transport NH3 and CO2. Glycerol facilitators function as solute nonspecific channels, and may transport glycerol, dihydroxyacetone, propanediol, urea and other small neutral molecules in physiologically importantprocesses. Some members of the family, including the yeast FPS protein (TC #1.A.8.5.1) and tobacco NtTIPA may transport both water and small solutes. [Transport and binding proteins, Unknown substrate]


Pssm-ID: 273306 [Multi-domain]  Cd Length: 216  Bit Score: 156.69  E-value: 1.05e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384   85 AEFVGTFILIFFA--TAAPIVNQKYGGAISPFGNAACA---GLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVP 159
Cdd:TIGR00861   1 AEFLGTFLLVFFGvgSALGVNVAGAYGAVGGGQFLGVAlafGLAVATLVYCVGGISGAHLNPAVTIALLLGRRFPLKRVP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  160 AYVAVQVLGSICAGFALKGVFHPFLSGG---VTVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTRAV--GELAGIAVG 234
Cdd:TIGR00861  81 VYIVAQLIGAILGAALLYGLTSGLFPGNlavNGSASAGVSSGQAFFVEFIGTAILVLVIFATTDDRNRVprGGFAPLAIG 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 125575384  235 AAVTLNILIAGPTTGGSMNPVRTLGPAVAAG--NYRQLWIYLIAPTLGAVAGAGVY 288
Cdd:TIGR00861 161 LLVFLIHLSMGPYTGTGMNPARSLGPALFAGlaGWGNHWVYWVGPIIGAILGALVY 216
 
Name Accession Description Interval E-value
PLN00026 PLN00026
aquaporin NIP; Provisional
 19-309 1.05e-167

aquaporin NIP; Provisional


Pssm-ID: 177663 [Multi-domain]  Cd Length: 298  Bit Score: 467.42  E-value: 1.05e-167
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  19 ASAPATPGTP-APLFAGPRVDSLSY-ERKSMPRCKCLPAaVAEAWAPSaHGCVVEIPAPDVSLTRKLGAEFVGTFILIFF 96
Cdd:PLN00026  10 PSTPATPGTPgGPLFGGFRVDSLSYgDRKLLPSCKCFPV-EALEWGRL-PTCFCDLPPPDVSLTRKLGAEFVGTFILIFA 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  97 ATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQVLGSICAGFAL 176
Cdd:PLN00026  88 ATAGPIVNQKYDGAETLIGNAACAGLAVMIVILSTGHISGAHLNPSLTIAFAALRHFPWKHVPAYIAAQVSASICASFAL 167

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 177 KGVFHPFLSGGVTVpdPTISTAQAFFTEFIITFNLLFVVTAVATDTRAVGELAGIAVGAAVTLNILIAGPTTGGSMNPVR 256
Cdd:PLN00026 168 KGVFHPFMSGGVTV--PSVSTGQAFALEFIITFNLLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVR 245

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|...
gi 125575384 257 TLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAVKLRDENGETPRPQRSFRR 309
Cdd:PLN00026 246 TLGPAVAAGNYRAIWIYLVAPTLGALAGAGTYTAVKLREEDGDPPRPVRSFRR 298
PLN00184 PLN00184
aquaporin NIP1; Provisional
 77-296 1.52e-67

aquaporin NIP1; Provisional


Pssm-ID: 177778  Cd Length: 296  Bit Score: 212.96  E-value: 1.52e-67
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  77 VSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWL 156
Cdd:PLN00184  51 VPFLQKLIAEVLGTYFLVFTGCASVVVNMQNDNVVTLPGIAIVWGLTIMVLIYSLGHISGAHINPAVTIAFASCGRFPLK 130

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 157 QVPAYVAVQVLGSICAGFALKGVF---HPFLSGGVTV---PDPTISTAQAFFTEFIITFNLLFVVTAVATDTRAVGELAG 230
Cdd:PLN00184 131 QVPAYVISQVIGSTLAAATLRLLFgldHDVCSGKHDVfigSSPVGSDLQAFVMEFIVTFYLMFIISGVATDNRAIGELAG 210

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 125575384 231 IAVGAAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAVKLRDE 296
Cdd:PLN00184 211 LAIGSTVLLNVLIAAPVSSASMNPGRSLGPAMVYGCYKGIWIYIVAPTLGAIAGAWVYNTVRYTDK 276
PLN00182 PLN00182
putative aquaporin NIP4; Provisional
 77-296 4.89e-64

putative aquaporin NIP4; Provisional


Pssm-ID: 165748  Cd Length: 283  Bit Score: 203.60  E-value: 4.89e-64
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  77 VSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWL 156
Cdd:PLN00182  39 VCLTQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHFNPAVTVTFAIFRRFPWY 118

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 157 QVPAYVAVQVLGSICAGFALKGVFH--PFLSGGVTvpdPTISTAQAFFTEFIITFNLLFVVTAVATDTRAVGELAGIAVG 234
Cdd:PLN00182 119 QVPLYIGAQLTGSLLASLTLRLMFNvtPEAFFGTT---PTDSSGQALVAEIIISFLLMFVISGVATDSRAVGELAGIAVG 195

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 125575384 235 AAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAVKLRDE 296
Cdd:PLN00182 196 MTIILNVFVAGPISGASMNPARSLGPAIVMGRYKGIWVYIVGPFVGIIAGGFVYNLIRFTDK 257
GlpF COG0580
Glycerol uptake facilitator or related aquaporin (Major Intrinsic protein Family) ...
 78-291 3.88e-60

Glycerol uptake facilitator or related aquaporin (Major Intrinsic protein Family) [Carbohydrate transport and metabolism];


Pssm-ID: 440345 [Multi-domain]  Cd Length: 232  Bit Score: 191.49  E-value: 3.88e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  78 SLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQ 157
Cdd:COG0580    1 SLMKRLLAEFLGTFILVLLGCGVVAVLAGTKGGIGWLGIALAWGLAVLAGVYAFGHISGAHLNPAVTLALAVAGRFPWKD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 158 VPAYVAVQVLGSICAGFALKGVFHP--------FLSGGV--TVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTRAVGE 227
Cdd:COG0580   81 VLPYIIAQLLGAFLGAALVYLIYGGhfdatedgGAKLGVfaTSPAIGYSLGAAFLTEIIGTFVLVFVILGITDKRAAPGG 160

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 228 LAGIAVGAAVTLNILIAGPTTGGSMNPVRTLGPAVAA------GNYRQLWIYLIAPTLGAVAGAGVYTAV 291
Cdd:COG0580  161 LAPLAIGLLVAAIGLSLGPLTGYAINPARDLGPRLFHalgkggSDWGYLWVPIVAPILGAALAALLYRAL 230
PLN00183 PLN00183
putative aquaporin NIP7; Provisional
 76-294 4.36e-58

putative aquaporin NIP7; Provisional


Pssm-ID: 215092  Cd Length: 274  Bit Score: 187.76  E-value: 4.36e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  76 DVSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPW 155
Cdd:PLN00183  41 DLNPARMVLAEMVGTFILMFCVCGIIASTQLSGGEVGLLEYAATAGLTVVVVVFSIGSISGAHVNPSVTIAFATFGHFPW 120

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 156 LQVPAYVAVQVLGSICAGFALKGVFHpfLSGGVTVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTRAVGELAGIAVGA 235
Cdd:PLN00183 121 SKVPLYITAQTVGSVLATYVGVSVYG--VKADLMTTKPAQGCSSAFWVEFIATFIVMFLAASLTSQPQSLGHLSGFVIGI 198

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 125575384 236 AVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAVKLR 294
Cdd:PLN00183 199 AIGLAVLITGPVSGGSMNPARSLGPAIVSWDFKDIWIYITAPTIGAVAGALMFHLLRLR 257
MIP cd00333
Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane ...
 81-288 4.50e-53

Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal cytosolic portion followed by six transmembrane helices, which might have arisen through gene duplication. On the basis of sequence similarity and functional characteristics, the superfamily can be subdivided into two major groups: water-selective channels called aquaporins (AQPs) and glycerol uptake facilitators (GlpFs). AQPs are found in all three kingdoms of life, while GlpFs have been characterized only within microorganisms.


Pssm-ID: 238204  Cd Length: 228  Bit Score: 173.21  E-value: 4.50e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  81 RKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACA-GLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVP 159
Cdd:cd00333    1 RKYLAEFLGTFLLVFFGCGSVLAVKLAGGASGGLLGIALAwGFAIFVLVYAVGHISGGHINPAVTLALAVGGRFPLIRVI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 160 AYVAVQVLGSICAGFALKGVF-----------HPFLSGGVTVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTR--AVG 226
Cdd:cd00333   81 PYIIAQLLGAILGAALLYGLYyglyleflganNIVAGIFGTYPSPGVSNGNAFFVEFIGTFILVLVVFATTDDPNgpPPG 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 125575384 227 ELAGIAVGAAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYR---QLWIYLIAPTLGAVAGAGVY 288
Cdd:cd00333  161 GLAPLAIGLLVAAIGLAGGPITGASMNPARSLGPALFTGLARhwhYFWVYWVGPLIGAIAGALVY 225
MIP pfam00230
Major intrinsic protein; MIP (Major Intrinsic Protein) family proteins exhibit essentially two ...
 77-288 1.45e-50

Major intrinsic protein; MIP (Major Intrinsic Protein) family proteins exhibit essentially two distinct types of channel properties: (1) specific water transport by the aquaporins, and (2) small neutral solutes transport, such as glycerol by the glycerol facilitators.


Pssm-ID: 395174 [Multi-domain]  Cd Length: 223  Bit Score: 166.72  E-value: 1.45e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384   77 VSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACA---GLAVTTIILSTGHISGAHLNPSLTIAFAALRHF 153
Cdd:pfam00230   5 VSFLRAVIAEFLATLLFVFFGVGSALGVKKLVSSLAVSGLLAVAlafGLALATLVYCAGHISGAHLNPAVTLALLVGRRF 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  154 PWLQVPAYVAVQVLGSICAGFALKGVFHPFL--SGGVTVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTRAV--GELA 229
Cdd:pfam00230  85 SLLRAIFYIVAQLLGAIAGAALLYGVTVGLQraGLFANTLAPGVNAGQAFVVEIILTFQLVYCVFATTDDRRNGslGTVA 164

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 125575384  230 GIAVGAAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVY 288
Cdd:pfam00230 165 PLAIGFAVFLNHLAGGPYTGASMNPARSFGPAVVLWKWDDHWVYWVGPFIGAALGALVY 223
MIP TIGR00861
MIP family channel proteins; 1.A.8 The Major Intrinsic Protein (MIP) FamilyThe MIP family is ...
 85-288 1.05e-46

MIP family channel proteins; 1.A.8 The Major Intrinsic Protein (MIP) FamilyThe MIP family is large and diverse, possessing over 100 members that all form transmembrane channels. These channel proteins function in water, smallcarbohydrate (e.g., glycerol), urea, NH3, CO2 and possibly ion transport by an energy independent mechanism. They are found ubiquitously in bacteria, archaeaand eukaryotes. The MIP family contains two major groups of channels: aquaporins and glycerol facilitators.The known aquaporins cluster loosely together as do the known glycerol facilitators. MIP family proteins are believed to form aqueous pores that selectively allow passive transport of their solute(s) across the membrane with minimal apparent recognition. Aquaporins selectively transport water (but not glycerol) while glycerol facilitators selectively transport glycerol but not water. Some aquaporins can transport NH3 and CO2. Glycerol facilitators function as solute nonspecific channels, and may transport glycerol, dihydroxyacetone, propanediol, urea and other small neutral molecules in physiologically importantprocesses. Some members of the family, including the yeast FPS protein (TC #1.A.8.5.1) and tobacco NtTIPA may transport both water and small solutes. [Transport and binding proteins, Unknown substrate]


Pssm-ID: 273306 [Multi-domain]  Cd Length: 216  Bit Score: 156.69  E-value: 1.05e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384   85 AEFVGTFILIFFA--TAAPIVNQKYGGAISPFGNAACA---GLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVP 159
Cdd:TIGR00861   1 AEFLGTFLLVFFGvgSALGVNVAGAYGAVGGGQFLGVAlafGLAVATLVYCVGGISGAHLNPAVTIALLLGRRFPLKRVP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  160 AYVAVQVLGSICAGFALKGVFHPFLSGG---VTVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTRAV--GELAGIAVG 234
Cdd:TIGR00861  81 VYIVAQLIGAILGAALLYGLTSGLFPGNlavNGSASAGVSSGQAFFVEFIGTAILVLVIFATTDDRNRVprGGFAPLAIG 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 125575384  235 AAVTLNILIAGPTTGGSMNPVRTLGPAVAAG--NYRQLWIYLIAPTLGAVAGAGVY 288
Cdd:TIGR00861 161 LLVFLIHLSMGPYTGTGMNPARSLGPALFAGlaGWGNHWVYWVGPIIGAILGALVY 216
PRK05420 PRK05420
aquaporin Z; Provisional
 79-291 7.55e-33

aquaporin Z; Provisional


Pssm-ID: 235453 [Multi-domain]  Cd Length: 231  Bit Score: 120.84  E-value: 7.55e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  79 LTRKLGAEFVGTFILIF-------FATAAPIVnqkyggAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALR 151
Cdd:PRK05420   1 MFKKLAAEFFGTFWLVFggcgsavLAAAFPEL------GIGFLGVALAFGLTVLTMAYAVGHISGGHFNPAVSVGLWAGG 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 152 HFPWLQVPAYVAVQVLGSICAGFAL------KGVFH----PFLSGGVTVPDP-TISTAQAFFTEFIITFNLLFVVTAvAT 220
Cdd:PRK05420  75 RFPAKELVPYIIAQVVGGIAAAAVLyliasgKAGFDlagsGFASNGYGEHSPgGYSLLAALVCEVVLTAFFLLVILG-AT 153

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 125575384 221 DTRAVGELAGIAVGAAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNY--RQLWIYLIAPTLGAVAGAGVYTAV 291
Cdd:PRK05420 154 DKRAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTGVALFVGGWalEQLWLFWVAPIVGAIIGGLIYRLL 226
PTZ00016 PTZ00016
aquaglyceroporin; Provisional
 81-288 1.60e-27

aquaglyceroporin; Provisional


Pssm-ID: 240230 [Multi-domain]  Cd Length: 294  Bit Score: 108.29  E-value: 1.60e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  81 RKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVPA 160
Cdd:PTZ00016  51 REYVAEFLGTFVLLFFGEGVVATTHTVGNNGDYLAITIGWGLGVTFGLLVSAGISGGHLNPAVTLGNAVFGKFPWKKLPG 130

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 161 YVAVQVLGSICAGFALKGVFHPFLS----------------GGVTVPDPTISTAQAFFTEFIITFNLLFVVTAV--ATDT 222
Cdd:PTZ00016 131 YFVAQLLGAFVGAACVYGLYYGFFKdhgipelpwetmangfAGVFATYPREGNFYAIFSELILTAILLLVILAItdPNNC 210

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 125575384 223 RAVGELAgIAVGAAVTLNILIAGPTTGGSMNPVRTLGPAV-----------AAGNYrQLWIYLIAPTLGAVAGAGVY 288
Cdd:PTZ00016 211 PAFNYLP-VAVGLLVFVIGISFGGNTGYALNPARDLGPRLfsailwgsevfTKDNY-YFWVPLVAPFVGAVLGMFLY 285
PLN00166 PLN00166
aquaporin TIP2; Provisional
 85-296 3.46e-26

aquaporin TIP2; Provisional


Pssm-ID: 165733  Cd Length: 250  Bit Score: 103.86  E-value: 3.46e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  85 AEFVGTFILIFFATAAPIVNQKY--GGAISPFGNAACA---GLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVP 159
Cdd:PLN00166  23 SEFIATLLFVFAGVGSAIAFAKLtsDAALDPAGLVAVAvahAFALFVGVSIAANISGGHLNPAVTLGLAIGGNITIITGF 102

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 160 AYVAVQVLGSICAGFALKgvfhpFLSGGVTVPDPTISTA----QAFFTEFIITFNLLFVVTAVATDTR--AVGELAGIAV 233
Cdd:PLN00166 103 FYWIAQCLGSIVACLLLV-----FVTNGESVPTHGVAAGlgaiEGVVMEIVVTFALVYTVYATAADPKkgSLGTIAPIAI 177

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 125575384 234 GAAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTL-GAVAG-------AGVYTAVKLRDE 296
Cdd:PLN00166 178 GFIVGANILAAGPFSGGSMNPARSFGPAVVSGDFSQIWIYWVGPLVgGALAGliygdvfIGSYAAAPTSED 248
PLN00027 PLN00027
aquaporin TIP; Provisional
 85-288 1.41e-19

aquaporin TIP; Provisional


Pssm-ID: 177664  Cd Length: 252  Bit Score: 85.99  E-value: 1.41e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  85 AEFVGTFILIFFATAAPIVNQKY--GGAISPFGNAACA---GLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVP 159
Cdd:PLN00027  25 AEFISTLIFVFAGEGSGMAFNKLtdNGSTTPAGLVAAAlahAFALFVAVSVGANISGGHVNPAVTFGAFIGGNITLLRGI 104

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 160 AYVAVQVLGSICAGFALKgvfhpFLSGGVTVPDPTISTA----QAFFTEFIITFNLLFVVTAVATDTR--AVGELAGIAV 233
Cdd:PLN00027 105 LYWIAQLLGSVVACLLLK-----FSTGGLETSAFSLSSGvgvwNAFVFEIVMTFGLVYTVYATAVDPKkgDLGIIAPIAI 179

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 125575384 234 GAAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVY 288
Cdd:PLN00027 180 GFIVGANILAGGAFDGASMNPAVSFGPAVVSWTWTNHWVYWAGPLIGGGIAGLVY 234
PLN00167 PLN00167
aquaporin TIP5; Provisional
 85-291 2.00e-15

aquaporin TIP5; Provisional


Pssm-ID: 215085  Cd Length: 256  Bit Score: 74.55  E-value: 2.00e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  85 AEFVGTFILIFFATAAPIVNQKY--GGAISPFG---NAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVP 159
Cdd:PLN00167  27 AEFISTFFFVFAAVGSAMSSRKLmpDAASDPSSlliVAIANAFALSSAVYIAANISGGHVNPAVTFGMAVGGHISVPTAM 106

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384 160 AYVAVQVLGSICAGFALKgvfhpFLSGGVTVPDPTISTAQAFF----TEFIITFNLLFVVTAVATDTRA-VGELAGIAVG 234
Cdd:PLN00167 107 FYWISQMLASVMACLFLK-----VTTVGQHVPTYKIAEEMTGFgasvLEGVLTFGLVYTVYAAGDPRRGlLGAIGPLAIG 181

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 125575384 235 AAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAV 291
Cdd:PLN00167 182 LVAGANVLAAGPFSGGSMNPACAFGSAVVAGSFKNQAVYWVGPLLGGAVAGLLYDNV 238
GlpF COG0580
Glycerol uptake facilitator or related aquaporin (Major Intrinsic protein Family) ...
 72-181 3.51e-07

Glycerol uptake facilitator or related aquaporin (Major Intrinsic protein Family) [Carbohydrate transport and metabolism];


Pssm-ID: 440345 [Multi-domain]  Cd Length: 232  Bit Score: 50.13  E-value: 3.51e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  72 IPAPDVSLTRKLGAEFVGTFIL---IFFATAAPIVNQKYGGAIspfgnaacAGLAVTTIILSTGHISGAHLNP--SLTIA 146
Cdd:COG0580  123 SPAIGYSLGAAFLTEIIGTFVLvfvILGITDKRAAPGGLAPLA--------IGLLVAAIGLSLGPLTGYAINParDLGPR 194

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 125575384 147 FAALRH---FPWLQVPAYVAVQVLGSICAGFALKGVFH 181
Cdd:COG0580  195 LFHALGkggSDWGYLWVPIVAPILGAALAALLYRALFG 232
MIP cd00333
Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane ...
 67-174 9.34e-07

Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal cytosolic portion followed by six transmembrane helices, which might have arisen through gene duplication. On the basis of sequence similarity and functional characteristics, the superfamily can be subdivided into two major groups: water-selective channels called aquaporins (AQPs) and glycerol uptake facilitators (GlpFs). AQPs are found in all three kingdoms of life, while GlpFs have been characterized only within microorganisms.


Pssm-ID: 238204  Cd Length: 228  Bit Score: 48.79  E-value: 9.34e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384  67 GCVVEIPAPDVSLTRKLGAEFVGTFILIF--FATAAPiVNQKYGGAISPFgnaaCAGLAVTTIILSTGHISGAHLNP--S 142
Cdd:cd00333  117 GIFGTYPSPGVSNGNAFFVEFIGTFILVLvvFATTDD-PNGPPPGGLAPL----AIGLLVAAIGLAGGPITGASMNParS 191

                         90       100       110
                 ....*....|....*....|....*....|..
gi 125575384 143 LTIAFAALRHFPWLQVPAYVAVQVLGSICAGF 174
Cdd:cd00333  192 LGPALFTGLARHWHYFWVYWVGPLIGAIAGAL 223
MIP TIGR00861
MIP family channel proteins; 1.A.8 The Major Intrinsic Protein (MIP) FamilyThe MIP family is ...
 69-174 4.30e-03

MIP family channel proteins; 1.A.8 The Major Intrinsic Protein (MIP) FamilyThe MIP family is large and diverse, possessing over 100 members that all form transmembrane channels. These channel proteins function in water, smallcarbohydrate (e.g., glycerol), urea, NH3, CO2 and possibly ion transport by an energy independent mechanism. They are found ubiquitously in bacteria, archaeaand eukaryotes. The MIP family contains two major groups of channels: aquaporins and glycerol facilitators.The known aquaporins cluster loosely together as do the known glycerol facilitators. MIP family proteins are believed to form aqueous pores that selectively allow passive transport of their solute(s) across the membrane with minimal apparent recognition. Aquaporins selectively transport water (but not glycerol) while glycerol facilitators selectively transport glycerol but not water. Some aquaporins can transport NH3 and CO2. Glycerol facilitators function as solute nonspecific channels, and may transport glycerol, dihydroxyacetone, propanediol, urea and other small neutral molecules in physiologically importantprocesses. Some members of the family, including the yeast FPS protein (TC #1.A.8.5.1) and tobacco NtTIPA may transport both water and small solutes. [Transport and binding proteins, Unknown substrate]


Pssm-ID: 273306 [Multi-domain]  Cd Length: 216  Bit Score: 37.66  E-value: 4.30e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 125575384   69 VVEIPAPDVSLTRKLGAEFVGTFILIF--FATAAPiVNQKYGGAISPFgnaaCAGLAVTTIILSTGHISGAHLNPSLTIA 146
Cdd:TIGR00861 111 VNGSASAGVSSGQAFFVEFIGTAILVLviFATTDD-RNRVPRGGFAPL----AIGLLVFLIHLSMGPYTGTGMNPARSLG 185

                          90       100
                  ....*....|....*....|....*....
gi 125575384  147 FA-ALRHFPWLQVPAYVAVQVLGSICAGF 174
Cdd:TIGR00861 186 PAlFAGLAGWGNHWVYWVGPIIGAILGAL 214
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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