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Conserved domains on  [gi|124015259|gb|ABM88759|]
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erythrocyte membrane protein 1 [Plasmodium falciparum]

Protein Classification

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
ATS pfam15445
acidic terminal segments, variant surface antigen of PfEMP1; ATS is the intracellular and ...
2597-3053 0e+00

acidic terminal segments, variant surface antigen of PfEMP1; ATS is the intracellular and relatively conserved acidic terminal segment of the Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1). this domain appears to be present in all variants of the highly polymorphic PfEMP1 proteins.


:

Pssm-ID: 373851 [Multi-domain]  Cd Length: 446  Bit Score: 790.16  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2597 TLAWSVGIGFATFTYFYLKKKTKSTI-DLLRVINIPKSDYDIPTKLSPNRYIPYTSGKYRGKRYIYLEGDSGTDS--GYT 2673
Cdd:pfam15445    1 TIPWSVGIAFAAFTYFFLKKKTKSSVgNLFQILQIPKGDYDIPTLKSKNRYIPYASDRYKGKTYIYMEGDSSGDEkyAFM 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2674 DHYSDITSSSESEYEeLDINDIYVPDGPKYKTLIEVVLEPSGklsgntiptSDKNTPSDTQNDIQNDDIPSS-KITDNEW 2752
Cdd:pfam15445   81 SDTTDITSSESEYEE-LDINDIYVPGSPKYKTLIEVVLEPSK---------NNTNTPSDTQNDIPSDDIPSTnKITDEEW 150
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2753 NTLKDEFISQYLQSEQpNDIPNDYTSGNSSTNTNITTMSRDNMEEKPFIMSIHDRNLYSGEEYNYNVNMSTNNV-DIPIN 2831
Cdd:pfam15445  151 NTLKHDFISNMLQNTQ-NDEPNILHSGNVPNNTHPNTLSRDNMEEKPFIMSIHDRNLYTGEEYSYNINMSTNSMdDIPKY 229
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2832 RDNNVYSGIDLINDTLSGNKHIDIYDEVLKRKENELFGTKHPKHTNTHNVTKPARDDPIHNQLNLFHKWLDRHRDMCEKW 2911
Cdd:pfam15445  230 VSNNVYSGIDLINDTLSGNQHIDIYDEVLKRKENELFGTNHTKHTSTNSVAKNTNSDPILNQLNLFHKWLDRHRDMCEKW 309
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2912 ENHHERLAKLKEKWENET-HSGNTHPSE----SNKTLNTDVSIQIHMDNPKPINEFtyvdsnpnqvddTYVDSNPDNSSM 2986
Cdd:pfam15445  310 KNKEERLDKLKEEWNKENnHSGDIHPSDdipsDNKVLNTDVSIQIDMDNPKPKNEF------------TNMDTNPDNSTM 377
                          410       420       430       440       450       460
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 124015259  2987 DTILEDLEKYNEPYYDV-QDDIYYDVNDQDVSTVDSNNMDVPSKVQIEMDINT-KLVKEKYPIADVWDI 3053
Cdd:pfam15445  378 DTILDDLEKYNEPYYDIyEDDIYYDVNDHDASTVDSNNMDVPSKVQIEMDVNNtKLVEEKYPISDVWNI 446
Duffy_binding pfam05424
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ...
116-301 2.97e-87

Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.


:

Pssm-ID: 428465 [Multi-domain]  Cd Length: 178  Bit Score: 282.62  E-value: 2.97e-87
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   116 ACAPYRRLHVCDQNLEQIEPIKITNTHNLLVDVCMAAKFEGQSITQDYPKYQATYGDSPSQICTMLARSFADIGDIVRGR 195
Cdd:pfam05424    1 ACAPPRRLHLCVKNLEKINSGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNTKYGDIPSEFCRQMAYSFADIGDIIRGK 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   196 DLYLGNPQEIKqrqqLENNLKTIFGKIYEKLNGAEaryGNDPEFFKLREDWWTANRETVWKAITCNAW-GNTYFHATCNR 274
Cdd:pfam05424   81 DLYLGNNKKKK----LEENLKKIFKKIYEKLTSKG---KDDGNYYQLREDWWEANREDIWKAMTCALTyGAKYFRKTCGD 153
                          170       180
                   ....*....|....*....|....*..
gi 124015259   275 GERTKGYCRcnDDQVPTYFDYVPQYLR 301
Cdd:pfam05424  154 GISPSTAGC--NDDVPTYFDYVPQFLR 178
PTZ00176 super family cl31413
erythrocyte membrane protein 1 (PfEMP1); Provisional
1-477 1.03e-73

erythrocyte membrane protein 1 (PfEMP1); Provisional


The actual alignment was detected with superfamily member PTZ00176:

Pssm-ID: 140204 [Multi-domain]  Cd Length: 1317  Bit Score: 273.06  E-value: 1.03e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259    1 MGPKEaaGGDDIEDESAKHMFDRIGKDVYDKVKEEAKERGKgLQGRLSEAKFEKNESDP-----QTPEDPCDLDHKYHTN 75
Cdd:PTZ00176    1 MALKK--GVINESKLSARNVLENIGNEIKDKRENESKYTDK-LKGSLWEARFSDGLSSSfgdvrSGYYDSCSLDHKFHTN 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   76 VTTNVI---NPCADRSDVRFSDEYGGQCTHNRIKDSQQGDNKGACAPYRRLHVCDQNLEQIEPIKITNTHNLLVDVCMAA 152
Cdd:PTZ00176   78 INNGYPparNPCDGRNQNRFDENGESYCNSDKIRGNENNSNAGACAPFRRQNMCDKNLEYLINKNTENTHDLLGNVLVTA 157
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  153 KFEGQSITQDYPKYQAtygdspSQICTMLARSFADIGDIVRGRDLYLGNPQEikqrqQLENNLKTIFGKIYEKL--NGAE 230
Cdd:PTZ00176  158 KYEGESIVNNHPDKDK------SSVCTALARSFADIGDIVRGKDMFKRNKHD-----NVENGLREVFKKIYEGLknNGAR 226
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  231 ARYGNDPE--FFKLREDWWTANRETVWKAITCNAWGNTYFHATCNRGERTKG----YCRCNDDQVPTYFDYVPQYLRWFE 304
Cdd:PTZ00176  227 EHYKEVKNgnYIKLREDWWTANRDQVWKAMTCVAPENAYFRKTEADGIGISSlilpYSKCGRDTDPPVVDYIPQRLRWMS 306
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  305 EWAEDFCrkknkkikdvkrNCRGKDKEDKDRYCSrngyDCEKTKRAIGKLRyGKQCISCLYACNPYVDWINNQKEQFDKQ 384
Cdd:PTZ00176  307 EWSEYFC------------NVLNKEIDEMNNQCK----DCEMSRRCNNDTE-GEKCKKCKEQCQIFKELVSKWKNQFDKQ 369
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  385 KKKYDE-------EIKKYENGA--SGGSRQKRDAGGTTTTNYDGYEKKFYdELNKSEYRTVDKFLEKLSNeeiCTKVkde 455
Cdd:PTZ00176  370 SMKYMElynkastNITKQNSSApeRGYRRNHRRRGYDDDTNVQLFLKKVI-ENNECKVESLGKYLDKTSH---CGNY--- 442
                         490       500
                  ....*....|....*....|..
gi 124015259  456 eggtidfkNVNSDSTSGASGTN 477
Cdd:PTZ00176  443 --------NFNYDNTPGSNRSN 456
PTZ00176 super family cl31413
erythrocyte membrane protein 1 (PfEMP1); Provisional
879-1629 3.88e-64

erythrocyte membrane protein 1 (PfEMP1); Provisional


The actual alignment was detected with superfamily member PTZ00176:

Pssm-ID: 140204 [Multi-domain]  Cd Length: 1317  Bit Score: 242.63  E-value: 3.88e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  879 LRGDISLAQFKNGRNGS-----TLKGQICKINEN-YSNDSRG--NSGGPCTGKDGDHGGVRMRIGTEWSNIEGKKQTSYK 950
Cdd:PTZ00176   40 LKGSLWEARFSDGLSSSfgdvrSGYYDSCSLDHKfHTNINNGypPARNPCDGRNQNRFDENGESYCNSDKIRGNENNSNA 119
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  951 NVFLPPRREHMCTSNLENLdvgsVTKNDKASHSLLGDVQLAAKTDAAEIIKRYKDqnniqltdpiqqKDQEAMCRAVRYS 1030
Cdd:PTZ00176  120 GACAPFRRQNMCDKNLEYL----INKNTENTHDLLGNVLVTAKYEGESIVNNHPD------------KDKSSVCTALARS 183
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1031 FADLGDIIRGRDMWDEDKSStDMETRLITVFKNIkekHDGIKDNP-KYTGDESKKPAYKKLRADWWEANRHQVWRAMKCA 1109
Cdd:PTZ00176  184 FADIGDIVRGKDMFKRNKHD-NVENGLREVFKKI---YEGLKNNGaREHYKEVKNGNYIKLREDWWTANRDQVWKAMTCV 259
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1110 T----------------KGIICP--------GMPVDDYIPQRLRWMTEWAEWYCKAQSQEYDKLKKISADC-MSKGDGKC 1164
Cdd:PTZ00176  260 ApenayfrkteadgigiSSLILPyskcgrdtDPPVVDYIPQRLRWMSEWSEYFCNVLNKEIDEMNNQCKDCeMSRRCNND 339
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1165 TQGDvDCGKCKAACDKYKEEIEKWNEQWRKISDKYNLLYLQAKT-----TSTNPGRTVLG-------DDDPDYQQMVDFL 1232
Cdd:PTZ00176  340 TEGE-KCKKCKEQCQIFKELVSKWKNQFDKQSMKYMELYNKASTnitkqNSSAPERGYRRnhrrrgyDDDTNVQLFLKKV 418
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1233 TPIHKASIAArvlvkraagspteiaaaapitpystaagyihqeigYGGCQEQTQFCeKKHGATSTSTTKENKEYTFKQPP 1312
Cdd:PTZ00176  419 IENNECKVES-----------------------------------LGKYLDKTSHC-GNYNFNYDNTPGSNRSNAFEITP 462
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1313 PEYATACDCINRSQTEE-PKKKEENVesaCKIVEKIlegkngrTTVGECNPKESYPDWDCK--NNIDISHDGACMPPRRQ 1389
Cdd:PTZ00176  463 EKFKKACKCKIPNPLEKcPNEENKNV---CTRFDKV-------YSCTSLSFKNDLSEWNNSgvKNKENDNNGVLVPPRRR 532
                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1390 KLCLYYIAHESQteNIKTDDNLKDAFIKTAAAETFLSWQYYkSKNDSEAkildrglipsqfLRSMMYTFGDYRDICLNTD 1469
Cdd:PTZ00176  533 NLCINLFSKKDY--KMKDENDFKEDLLNAAFSQGKLLGKKY-SNYSNEA------------YEAMKFSYADYSDIVKGTD 597
                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1470 IpkkQNDVAKAKDKIGKFFsKDGSKSPSGLSRQEWWKTNGPEIWKGMLCAltkYVTDTDNKrkikndysydKVNQSQNGN 1549
Cdd:PTZ00176  598 M---MNDLKKLNKELNTLL-KETEKGDISVDRKTWWDDNKNVVWNAMLCG---YKTENENQ----------QLNSSWCNV 660
                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1550 PSLEEFaakPQFLRWMIEWGEEFCAERQKKENIIKDACNEINSTQQCNDAKHRCNQACRA----YQEYVENKKKEFSGQT 1625
Cdd:PTZ00176  661 PDDDNI---DQFLRWLTEWAQQYCKEKLIKAHIINTKCKDIVEGRKHKSMVDITDVECKRlfidYEEWFRYRYNQWKGLS 737

                  ....
gi 124015259 1626 NNFV 1629
Cdd:PTZ00176  738 EKYI 741
Duffy_binding pfam05424
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ...
1782-2017 3.05e-41

Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.


:

Pssm-ID: 428465 [Multi-domain]  Cd Length: 178  Bit Score: 150.89  E-value: 3.05e-41
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  1782 ICIPPRRRRLYVGKLQEWATalpqgegaasPSHSRADDLRNAFIQSAAIETFFLWDRYKEEKKpqgdgsqqalsqltsty 1861
Cdd:pfam05424    1 ACAPPRRLHLCVKNLEKINS----------GNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNT----------------- 53
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  1862 sddeedppdkllQNGKIPTDFLRQMFYTLGDYRDIlVRGGSNTNGDTKDggdssnnnnivflasgkenkeamKKIQEQLK 1941
Cdd:pfam05424   54 ------------KYGDIPSEFCRQMAYSFADIGDI-IRGKDLYLGNNKK-----------------------KKLEENLK 97
                          170       180       190       200       210       220       230
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 124015259  1942 VFFSNSGNQPSTGGKNLSQSRDTPSSWWQKNGEHIWKGMICALTYkeNEEKTNDTQKIKKDEQVYEKIFGKPPNND 2017
Cdd:pfam05424   98 KIFKKIYEKLTSKGKDDGNYYQLREDWWEANREDIWKAMTCALTY--GAKYFRKTCGDGISPSTAGCNDDVPTYFD 171
PFEMP pfam03011
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ...
2333-2468 7.63e-36

PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.


:

Pssm-ID: 281064  Cd Length: 154  Bit Score: 134.55  E-value: 7.63e-36
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2333 LVKRWVEYFFEDYNKIKHKISHRIKNGEISPCIK------NCVEKWVDQKRKEWKEITERFKDQYKNDNSDDD------N 2400
Cdd:pfam03011    1 LFKRWVEYFLEDSIKWRKKLKSCINNGKGKCCKKeckndcECFKKWVEKKKEEWKKIKEHFLKQYKLKGLTGKtldeylD 80
                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 124015259  2401 VRSFLETLIPQITDANAKNKV---IKLSKFGNSCGCSASANEQnKNGEYKDAIDCMLKKLKDKIGECEKKH 2468
Cdd:pfam03011   81 LKSFLETFLFYIDIKEAYGDVkelKKLEEILDEEGCSAGAESA-KNNKNEDAIDKLLDKEEKKANNCKEKH 150
PFEMP super family cl03834
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ...
648-815 5.82e-30

PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.


The actual alignment was detected with superfamily member pfam03011:

Pssm-ID: 281064  Cd Length: 154  Bit Score: 117.60  E-value: 5.82e-30
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   648 FFYYWVAHMLKDSIHWKKKLQRCLQNGNRiKCGNNKCNNDCECFKRWITQKKDEWGKIVQHFKTQ-NIKGRGGSDntael 726
Cdd:pfam03011    1 LFKRWVEYFLEDSIKWRKKLKSCINNGKG-KCCKKECKNDCECFKKWVEKKKEEWKKIKEHFLKQyKLKGLTGKT----- 74
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   727 ipfdhdyvlqynLQEEFLKGDSEDASEEKSENSLDAEEAEELKHLREIIESEDNNQEASVGGGvTEQKNIMDKLLNYEKD 806
Cdd:pfam03011   75 ------------LDEYLDLKSFLETFLFYIDIKEAYGDVKELKKLEEILDEEGCSAGAESAKN-NKNEDAIDKLLDKEEK 141

                   ....*....
gi 124015259   807 EADLCLEIH 815
Cdd:pfam03011  142 KANNCKEKH 150
CIDR1_gamma pfam18562
Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind ...
2264-2317 9.28e-15

Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind uninfected RBCs, which is consistently associated with severe malaria in African children. The rosette-forming PfEMP1 adhesins, namely IT4/R29, Palo Alto 89F5 VarO, 3D7/PF13_0003 and IT4/var60, belong to a specific sub-group called groupA/UpsA var genes and all four present a specific Duffy Binding-Like and and Cysteine-Rich Interdomain Region (DBL1alpha1-CIDR1gamma) double domain Head region found at the extracellular region of PfEMP1. This entry represents the CIDR1gamma domain which increases the binding affinity to VarO (Palo Alto VarO parasites).


:

Pssm-ID: 408346  Cd Length: 52  Bit Score: 70.76  E-value: 9.28e-15
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....
gi 124015259  2264 STVLEMRVSADSKSGFnGDGLENaCRGAGIFEGIRKDEWKCRNVCGYVVCKPEN 2317
Cdd:pfam18562    1 TTTIDMLVSDNRGIKF-ENDLKE-CKEAGIFKGIRKDQWKCGKVCGYDVCKLKN 52
 
Name Accession Description Interval E-value
ATS pfam15445
acidic terminal segments, variant surface antigen of PfEMP1; ATS is the intracellular and ...
2597-3053 0e+00

acidic terminal segments, variant surface antigen of PfEMP1; ATS is the intracellular and relatively conserved acidic terminal segment of the Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1). this domain appears to be present in all variants of the highly polymorphic PfEMP1 proteins.


Pssm-ID: 373851 [Multi-domain]  Cd Length: 446  Bit Score: 790.16  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2597 TLAWSVGIGFATFTYFYLKKKTKSTI-DLLRVINIPKSDYDIPTKLSPNRYIPYTSGKYRGKRYIYLEGDSGTDS--GYT 2673
Cdd:pfam15445    1 TIPWSVGIAFAAFTYFFLKKKTKSSVgNLFQILQIPKGDYDIPTLKSKNRYIPYASDRYKGKTYIYMEGDSSGDEkyAFM 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2674 DHYSDITSSSESEYEeLDINDIYVPDGPKYKTLIEVVLEPSGklsgntiptSDKNTPSDTQNDIQNDDIPSS-KITDNEW 2752
Cdd:pfam15445   81 SDTTDITSSESEYEE-LDINDIYVPGSPKYKTLIEVVLEPSK---------NNTNTPSDTQNDIPSDDIPSTnKITDEEW 150
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2753 NTLKDEFISQYLQSEQpNDIPNDYTSGNSSTNTNITTMSRDNMEEKPFIMSIHDRNLYSGEEYNYNVNMSTNNV-DIPIN 2831
Cdd:pfam15445  151 NTLKHDFISNMLQNTQ-NDEPNILHSGNVPNNTHPNTLSRDNMEEKPFIMSIHDRNLYTGEEYSYNINMSTNSMdDIPKY 229
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2832 RDNNVYSGIDLINDTLSGNKHIDIYDEVLKRKENELFGTKHPKHTNTHNVTKPARDDPIHNQLNLFHKWLDRHRDMCEKW 2911
Cdd:pfam15445  230 VSNNVYSGIDLINDTLSGNQHIDIYDEVLKRKENELFGTNHTKHTSTNSVAKNTNSDPILNQLNLFHKWLDRHRDMCEKW 309
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2912 ENHHERLAKLKEKWENET-HSGNTHPSE----SNKTLNTDVSIQIHMDNPKPINEFtyvdsnpnqvddTYVDSNPDNSSM 2986
Cdd:pfam15445  310 KNKEERLDKLKEEWNKENnHSGDIHPSDdipsDNKVLNTDVSIQIDMDNPKPKNEF------------TNMDTNPDNSTM 377
                          410       420       430       440       450       460
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 124015259  2987 DTILEDLEKYNEPYYDV-QDDIYYDVNDQDVSTVDSNNMDVPSKVQIEMDINT-KLVKEKYPIADVWDI 3053
Cdd:pfam15445  378 DTILDDLEKYNEPYYDIyEDDIYYDVNDHDASTVDSNNMDVPSKVQIEMDVNNtKLVEEKYPISDVWNI 446
PTZ00176 PTZ00176
erythrocyte membrane protein 1 (PfEMP1); Provisional
2603-3053 5.85e-144

erythrocyte membrane protein 1 (PfEMP1); Provisional


Pssm-ID: 140204 [Multi-domain]  Cd Length: 1317  Bit Score: 487.24  E-value: 5.85e-144
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 2603 GIGFAT---FTYFYLKKKTKSTIDLLRVINIPKSDYDIPTKLSPNRYIPYTSGKYRGKRYIYLEGDSGTDS-GYTDHYSD 2678
Cdd:PTZ00176  894 GIGVALtlgLLLFKMRRKAKRQVDMIRILQMSQNEYGIPTTKSPNKYVPYGSQRYKGKTYLYVEGDTDEEKyMFMSDTTD 973
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 2679 ITSSSESEYEeLDINDIYVPDGPKYKTLIEVVLEPSgklsgntiptsdkntPSDTQNDIQNDDIPSSKITDNEWNTLKDE 2758
Cdd:PTZ00176  974 ITSSESEYEE-MDINDIYVPGSPKYKTLIEVVLEPS---------------KRDTQNDIPSDNTPSYKLTDEEWNQLKDD 1037
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 2759 FISQYLQSEQPNdipNDYTSGNSSTNTNITTMSRDNMEEKPFIMSIHDRNLYSGEEYNYNVNMSTNNVDIPINRDNNVYS 2838
Cdd:PTZ00176 1038 FISQYLPNTEPN---NNYRSGNSPTNTNNTTTSHDNMGEKPFIMSIHDRNLYTGEEISYNINMSTNTMDDPKYVSNNVYS 1114
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 2839 GIDLINDTLSGNKHIDIYDEVLKRKENELFGTKHPKHTNTHNVTK-PARDDPIHNQLNLFHKWLDRHRDMCEKWENHHER 2917
Cdd:PTZ00176 1115 GIDLINDTLSGNQHIDIYDEVLKRKENELFGTNHVKQTSIHSVAKnTYSDDAITNKINLFHKWLDRHRDMCEKWENHHER 1194
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 2918 LAKLKEKWENETHSGNThPSEsNKTLNTDVSIQIHMDNPKPINEFtyvdSNpnqvddtyVDSNPDNSSMDtiledlekyn 2997
Cdd:PTZ00176 1195 LAKLKEKWENDNDGGNV-PSD-NHVLNTDVSIEIDMDNPKPINQF----SN--------MDINVDTPTMD---------- 1250
                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 124015259 2998 epyyDVQDDIYYDVNDQDVS---------TVDSN--NMDVPSKVQIEMDI--NTK--LVKEKYPIADVWDI 3053
Cdd:PTZ00176 1251 ----NMEDDIYYDVNDNDDDndqpsvydiPMDHNkvDVDVPKKVHIEMKIlnNTSngSLEQQFPISDVWNI 1317
Duffy_binding pfam05424
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ...
116-301 2.97e-87

Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.


Pssm-ID: 428465 [Multi-domain]  Cd Length: 178  Bit Score: 282.62  E-value: 2.97e-87
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   116 ACAPYRRLHVCDQNLEQIEPIKITNTHNLLVDVCMAAKFEGQSITQDYPKYQATYGDSPSQICTMLARSFADIGDIVRGR 195
Cdd:pfam05424    1 ACAPPRRLHLCVKNLEKINSGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNTKYGDIPSEFCRQMAYSFADIGDIIRGK 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   196 DLYLGNPQEIKqrqqLENNLKTIFGKIYEKLNGAEaryGNDPEFFKLREDWWTANRETVWKAITCNAW-GNTYFHATCNR 274
Cdd:pfam05424   81 DLYLGNNKKKK----LEENLKKIFKKIYEKLTSKG---KDDGNYYQLREDWWEANREDIWKAMTCALTyGAKYFRKTCGD 153
                          170       180
                   ....*....|....*....|....*..
gi 124015259   275 GERTKGYCRcnDDQVPTYFDYVPQYLR 301
Cdd:pfam05424  154 GISPSTAGC--NDDVPTYFDYVPQFLR 178
PTZ00176 PTZ00176
erythrocyte membrane protein 1 (PfEMP1); Provisional
1-477 1.03e-73

erythrocyte membrane protein 1 (PfEMP1); Provisional


Pssm-ID: 140204 [Multi-domain]  Cd Length: 1317  Bit Score: 273.06  E-value: 1.03e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259    1 MGPKEaaGGDDIEDESAKHMFDRIGKDVYDKVKEEAKERGKgLQGRLSEAKFEKNESDP-----QTPEDPCDLDHKYHTN 75
Cdd:PTZ00176    1 MALKK--GVINESKLSARNVLENIGNEIKDKRENESKYTDK-LKGSLWEARFSDGLSSSfgdvrSGYYDSCSLDHKFHTN 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   76 VTTNVI---NPCADRSDVRFSDEYGGQCTHNRIKDSQQGDNKGACAPYRRLHVCDQNLEQIEPIKITNTHNLLVDVCMAA 152
Cdd:PTZ00176   78 INNGYPparNPCDGRNQNRFDENGESYCNSDKIRGNENNSNAGACAPFRRQNMCDKNLEYLINKNTENTHDLLGNVLVTA 157
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  153 KFEGQSITQDYPKYQAtygdspSQICTMLARSFADIGDIVRGRDLYLGNPQEikqrqQLENNLKTIFGKIYEKL--NGAE 230
Cdd:PTZ00176  158 KYEGESIVNNHPDKDK------SSVCTALARSFADIGDIVRGKDMFKRNKHD-----NVENGLREVFKKIYEGLknNGAR 226
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  231 ARYGNDPE--FFKLREDWWTANRETVWKAITCNAWGNTYFHATCNRGERTKG----YCRCNDDQVPTYFDYVPQYLRWFE 304
Cdd:PTZ00176  227 EHYKEVKNgnYIKLREDWWTANRDQVWKAMTCVAPENAYFRKTEADGIGISSlilpYSKCGRDTDPPVVDYIPQRLRWMS 306
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  305 EWAEDFCrkknkkikdvkrNCRGKDKEDKDRYCSrngyDCEKTKRAIGKLRyGKQCISCLYACNPYVDWINNQKEQFDKQ 384
Cdd:PTZ00176  307 EWSEYFC------------NVLNKEIDEMNNQCK----DCEMSRRCNNDTE-GEKCKKCKEQCQIFKELVSKWKNQFDKQ 369
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  385 KKKYDE-------EIKKYENGA--SGGSRQKRDAGGTTTTNYDGYEKKFYdELNKSEYRTVDKFLEKLSNeeiCTKVkde 455
Cdd:PTZ00176  370 SMKYMElynkastNITKQNSSApeRGYRRNHRRRGYDDDTNVQLFLKKVI-ENNECKVESLGKYLDKTSH---CGNY--- 442
                         490       500
                  ....*....|....*....|..
gi 124015259  456 eggtidfkNVNSDSTSGASGTN 477
Cdd:PTZ00176  443 --------NFNYDNTPGSNRSN 456
PTZ00176 PTZ00176
erythrocyte membrane protein 1 (PfEMP1); Provisional
879-1629 3.88e-64

erythrocyte membrane protein 1 (PfEMP1); Provisional


Pssm-ID: 140204 [Multi-domain]  Cd Length: 1317  Bit Score: 242.63  E-value: 3.88e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  879 LRGDISLAQFKNGRNGS-----TLKGQICKINEN-YSNDSRG--NSGGPCTGKDGDHGGVRMRIGTEWSNIEGKKQTSYK 950
Cdd:PTZ00176   40 LKGSLWEARFSDGLSSSfgdvrSGYYDSCSLDHKfHTNINNGypPARNPCDGRNQNRFDENGESYCNSDKIRGNENNSNA 119
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  951 NVFLPPRREHMCTSNLENLdvgsVTKNDKASHSLLGDVQLAAKTDAAEIIKRYKDqnniqltdpiqqKDQEAMCRAVRYS 1030
Cdd:PTZ00176  120 GACAPFRRQNMCDKNLEYL----INKNTENTHDLLGNVLVTAKYEGESIVNNHPD------------KDKSSVCTALARS 183
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1031 FADLGDIIRGRDMWDEDKSStDMETRLITVFKNIkekHDGIKDNP-KYTGDESKKPAYKKLRADWWEANRHQVWRAMKCA 1109
Cdd:PTZ00176  184 FADIGDIVRGKDMFKRNKHD-NVENGLREVFKKI---YEGLKNNGaREHYKEVKNGNYIKLREDWWTANRDQVWKAMTCV 259
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1110 T----------------KGIICP--------GMPVDDYIPQRLRWMTEWAEWYCKAQSQEYDKLKKISADC-MSKGDGKC 1164
Cdd:PTZ00176  260 ApenayfrkteadgigiSSLILPyskcgrdtDPPVVDYIPQRLRWMSEWSEYFCNVLNKEIDEMNNQCKDCeMSRRCNND 339
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1165 TQGDvDCGKCKAACDKYKEEIEKWNEQWRKISDKYNLLYLQAKT-----TSTNPGRTVLG-------DDDPDYQQMVDFL 1232
Cdd:PTZ00176  340 TEGE-KCKKCKEQCQIFKELVSKWKNQFDKQSMKYMELYNKASTnitkqNSSAPERGYRRnhrrrgyDDDTNVQLFLKKV 418
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1233 TPIHKASIAArvlvkraagspteiaaaapitpystaagyihqeigYGGCQEQTQFCeKKHGATSTSTTKENKEYTFKQPP 1312
Cdd:PTZ00176  419 IENNECKVES-----------------------------------LGKYLDKTSHC-GNYNFNYDNTPGSNRSNAFEITP 462
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1313 PEYATACDCINRSQTEE-PKKKEENVesaCKIVEKIlegkngrTTVGECNPKESYPDWDCK--NNIDISHDGACMPPRRQ 1389
Cdd:PTZ00176  463 EKFKKACKCKIPNPLEKcPNEENKNV---CTRFDKV-------YSCTSLSFKNDLSEWNNSgvKNKENDNNGVLVPPRRR 532
                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1390 KLCLYYIAHESQteNIKTDDNLKDAFIKTAAAETFLSWQYYkSKNDSEAkildrglipsqfLRSMMYTFGDYRDICLNTD 1469
Cdd:PTZ00176  533 NLCINLFSKKDY--KMKDENDFKEDLLNAAFSQGKLLGKKY-SNYSNEA------------YEAMKFSYADYSDIVKGTD 597
                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1470 IpkkQNDVAKAKDKIGKFFsKDGSKSPSGLSRQEWWKTNGPEIWKGMLCAltkYVTDTDNKrkikndysydKVNQSQNGN 1549
Cdd:PTZ00176  598 M---MNDLKKLNKELNTLL-KETEKGDISVDRKTWWDDNKNVVWNAMLCG---YKTENENQ----------QLNSSWCNV 660
                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1550 PSLEEFaakPQFLRWMIEWGEEFCAERQKKENIIKDACNEINSTQQCNDAKHRCNQACRA----YQEYVENKKKEFSGQT 1625
Cdd:PTZ00176  661 PDDDNI---DQFLRWLTEWAQQYCKEKLIKAHIINTKCKDIVEGRKHKSMVDITDVECKRlfidYEEWFRYRYNQWKGLS 737

                  ....
gi 124015259 1626 NNFV 1629
Cdd:PTZ00176  738 EKYI 741
Duffy_binding pfam05424
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ...
1382-1563 1.96e-46

Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.


Pssm-ID: 428465 [Multi-domain]  Cd Length: 178  Bit Score: 165.91  E-value: 1.96e-46
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  1382 ACMPPRRQKLCLYYIAHEsQTENIKTDDNLKDAFIKTAAAETFLSWQYYKSKNDSEakildrGLIPSQFLRSMMYTFGDY 1461
Cdd:pfam05424    1 ACAPPRRLHLCVKNLEKI-NSGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNTKY------GDIPSEFCRQMAYSFADI 73
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  1462 RDICLNTDIPKKQNDVAKAKDKIGKFFSKDGSKSPSGLS--------RQEWWKTNGPEIWKGMLCALTKyvtdtDNKRKI 1533
Cdd:pfam05424   74 GDIIRGKDLYLGNNKKKKLEENLKKIFKKIYEKLTSKGKddgnyyqlREDWWEANREDIWKAMTCALTY-----GAKYFR 148
                          170       180       190
                   ....*....|....*....|....*....|
gi 124015259  1534 KNDYSYDKVNQSQNGNPSLEEFAAKPQFLR 1563
Cdd:pfam05424  149 KTCGDGISPSTAGCNDDVPTYFDYVPQFLR 178
Duffy_binding pfam05424
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ...
1782-2017 3.05e-41

Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.


Pssm-ID: 428465 [Multi-domain]  Cd Length: 178  Bit Score: 150.89  E-value: 3.05e-41
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  1782 ICIPPRRRRLYVGKLQEWATalpqgegaasPSHSRADDLRNAFIQSAAIETFFLWDRYKEEKKpqgdgsqqalsqltsty 1861
Cdd:pfam05424    1 ACAPPRRLHLCVKNLEKINS----------GNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNT----------------- 53
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  1862 sddeedppdkllQNGKIPTDFLRQMFYTLGDYRDIlVRGGSNTNGDTKDggdssnnnnivflasgkenkeamKKIQEQLK 1941
Cdd:pfam05424   54 ------------KYGDIPSEFCRQMAYSFADIGDI-IRGKDLYLGNNKK-----------------------KKLEENLK 97
                          170       180       190       200       210       220       230
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 124015259  1942 VFFSNSGNQPSTGGKNLSQSRDTPSSWWQKNGEHIWKGMICALTYkeNEEKTNDTQKIKKDEQVYEKIFGKPPNND 2017
Cdd:pfam05424   98 KIFKKIYEKLTSKGKDDGNYYQLREDWWEANREDIWKAMTCALTY--GAKYFRKTCGDGISPSTAGCNDDVPTYFD 171
PFEMP pfam03011
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ...
2333-2468 7.63e-36

PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.


Pssm-ID: 281064  Cd Length: 154  Bit Score: 134.55  E-value: 7.63e-36
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2333 LVKRWVEYFFEDYNKIKHKISHRIKNGEISPCIK------NCVEKWVDQKRKEWKEITERFKDQYKNDNSDDD------N 2400
Cdd:pfam03011    1 LFKRWVEYFLEDSIKWRKKLKSCINNGKGKCCKKeckndcECFKKWVEKKKEEWKKIKEHFLKQYKLKGLTGKtldeylD 80
                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 124015259  2401 VRSFLETLIPQITDANAKNKV---IKLSKFGNSCGCSASANEQnKNGEYKDAIDCMLKKLKDKIGECEKKH 2468
Cdd:pfam03011   81 LKSFLETFLFYIDIKEAYGDVkelKKLEEILDEEGCSAGAESA-KNNKNEDAIDKLLDKEEKKANNCKEKH 150
PFEMP pfam03011
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ...
648-815 5.82e-30

PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.


Pssm-ID: 281064  Cd Length: 154  Bit Score: 117.60  E-value: 5.82e-30
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   648 FFYYWVAHMLKDSIHWKKKLQRCLQNGNRiKCGNNKCNNDCECFKRWITQKKDEWGKIVQHFKTQ-NIKGRGGSDntael 726
Cdd:pfam03011    1 LFKRWVEYFLEDSIKWRKKLKSCINNGKG-KCCKKECKNDCECFKKWVEKKKEEWKKIKEHFLKQyKLKGLTGKT----- 74
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   727 ipfdhdyvlqynLQEEFLKGDSEDASEEKSENSLDAEEAEELKHLREIIESEDNNQEASVGGGvTEQKNIMDKLLNYEKD 806
Cdd:pfam03011   75 ------------LDEYLDLKSFLETFLFYIDIKEAYGDVKELKKLEEILDEEGCSAGAESAKN-NKNEDAIDKLLDKEEK 141

                   ....*....
gi 124015259   807 EADLCLEIH 815
Cdd:pfam03011  142 KANNCKEKH 150
CIDR1_gamma pfam18562
Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind ...
2264-2317 9.28e-15

Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind uninfected RBCs, which is consistently associated with severe malaria in African children. The rosette-forming PfEMP1 adhesins, namely IT4/R29, Palo Alto 89F5 VarO, 3D7/PF13_0003 and IT4/var60, belong to a specific sub-group called groupA/UpsA var genes and all four present a specific Duffy Binding-Like and and Cysteine-Rich Interdomain Region (DBL1alpha1-CIDR1gamma) double domain Head region found at the extracellular region of PfEMP1. This entry represents the CIDR1gamma domain which increases the binding affinity to VarO (Palo Alto VarO parasites).


Pssm-ID: 408346  Cd Length: 52  Bit Score: 70.76  E-value: 9.28e-15
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....
gi 124015259  2264 STVLEMRVSADSKSGFnGDGLENaCRGAGIFEGIRKDEWKCRNVCGYVVCKPEN 2317
Cdd:pfam18562    1 TTTIDMLVSDNRGIKF-ENDLKE-CKEAGIFKGIRKDQWKCGKVCGYDVCKLKN 52
NTS pfam15447
N-terminal segments of PfEMP1; This family, the N-terminal segment, is the most variable part ...
16-51 4.79e-11

N-terminal segments of PfEMP1; This family, the N-terminal segment, is the most variable part of the variant surface antigen family of Plasmodium falciparum, the erythrocyte membrane protein-1 (PfEMP1) proteins. PfEMP1 is an important target for protective immunity and is implicated in the pathology of malaria through its ability to adhere to host endothelial receptors. A structural and functional study of the N-terminal domain of PfEMP1 from the VarO variant comprising the N-terminal segment (NTS) and the first DBL domain (DBL1alpha1), shows this region is directly implicated in rosetting. NTS, previously thought to be a structurally independent component of PfEMP1, forms an integral part of the DBL1alpha domain that is found to be the important heparin-binding site. This family is closely associated with PFEMP, pfam03011, and Duffy_binding, pfam05424.


Pssm-ID: 406013 [Multi-domain]  Cd Length: 36  Bit Score: 59.72  E-value: 4.79e-11
                           10        20        30
                   ....*....|....*....|....*....|....*.
gi 124015259    16 SAKHMFDRIGKDVYDKVKEEAKERGKGLQGRLSEAK 51
Cdd:pfam15447    1 SAKHLLDRIGKDVHDKVKKEAKRYKSELKGDLSKAK 36
PTZ00176 PTZ00176
erythrocyte membrane protein 1 (PfEMP1); Provisional
108-312 5.19e-10

erythrocyte membrane protein 1 (PfEMP1); Provisional


Pssm-ID: 140204 [Multi-domain]  Cd Length: 1317  Bit Score: 65.83  E-value: 5.19e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  108 SQQGDNKGACAPYRRLHVCdQNLEQIEPIKITNTHNLLVDVCMAAKFEGQSITQDYPKYQatygdspSQICTMLARSFAD 187
Cdd:PTZ00176  517 NKENDNNGVLVPPRRRNLC-INLFSKKDYKMKDENDFKEDLLNAAFSQGKLLGKKYSNYS-------NEAYEAMKFSYAD 588
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  188 IGDIVRGRDLYlgnpqeikqrqqleNNLKtifgKIYEKLNG--AEARYGndpEFFKLREDWWTANRETVWKAITCNawgn 265
Cdd:PTZ00176  589 YSDIVKGTDMM--------------NDLK----KLNKELNTllKETEKG---DISVDRKTWWDDNKNVVWNAMLCG---- 643
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 124015259  266 tyfHATCNRGER-TKGYCRCNDDqvptyfDYVPQYLRWFEEWAEDFCR 312
Cdd:PTZ00176  644 ---YKTENENQQlNSSWCNVPDD------DNIDQFLRWLTEWAQQYCK 682
 
Name Accession Description Interval E-value
ATS pfam15445
acidic terminal segments, variant surface antigen of PfEMP1; ATS is the intracellular and ...
2597-3053 0e+00

acidic terminal segments, variant surface antigen of PfEMP1; ATS is the intracellular and relatively conserved acidic terminal segment of the Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1). this domain appears to be present in all variants of the highly polymorphic PfEMP1 proteins.


Pssm-ID: 373851 [Multi-domain]  Cd Length: 446  Bit Score: 790.16  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2597 TLAWSVGIGFATFTYFYLKKKTKSTI-DLLRVINIPKSDYDIPTKLSPNRYIPYTSGKYRGKRYIYLEGDSGTDS--GYT 2673
Cdd:pfam15445    1 TIPWSVGIAFAAFTYFFLKKKTKSSVgNLFQILQIPKGDYDIPTLKSKNRYIPYASDRYKGKTYIYMEGDSSGDEkyAFM 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2674 DHYSDITSSSESEYEeLDINDIYVPDGPKYKTLIEVVLEPSGklsgntiptSDKNTPSDTQNDIQNDDIPSS-KITDNEW 2752
Cdd:pfam15445   81 SDTTDITSSESEYEE-LDINDIYVPGSPKYKTLIEVVLEPSK---------NNTNTPSDTQNDIPSDDIPSTnKITDEEW 150
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2753 NTLKDEFISQYLQSEQpNDIPNDYTSGNSSTNTNITTMSRDNMEEKPFIMSIHDRNLYSGEEYNYNVNMSTNNV-DIPIN 2831
Cdd:pfam15445  151 NTLKHDFISNMLQNTQ-NDEPNILHSGNVPNNTHPNTLSRDNMEEKPFIMSIHDRNLYTGEEYSYNINMSTNSMdDIPKY 229
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2832 RDNNVYSGIDLINDTLSGNKHIDIYDEVLKRKENELFGTKHPKHTNTHNVTKPARDDPIHNQLNLFHKWLDRHRDMCEKW 2911
Cdd:pfam15445  230 VSNNVYSGIDLINDTLSGNQHIDIYDEVLKRKENELFGTNHTKHTSTNSVAKNTNSDPILNQLNLFHKWLDRHRDMCEKW 309
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2912 ENHHERLAKLKEKWENET-HSGNTHPSE----SNKTLNTDVSIQIHMDNPKPINEFtyvdsnpnqvddTYVDSNPDNSSM 2986
Cdd:pfam15445  310 KNKEERLDKLKEEWNKENnHSGDIHPSDdipsDNKVLNTDVSIQIDMDNPKPKNEF------------TNMDTNPDNSTM 377
                          410       420       430       440       450       460
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 124015259  2987 DTILEDLEKYNEPYYDV-QDDIYYDVNDQDVSTVDSNNMDVPSKVQIEMDINT-KLVKEKYPIADVWDI 3053
Cdd:pfam15445  378 DTILDDLEKYNEPYYDIyEDDIYYDVNDHDASTVDSNNMDVPSKVQIEMDVNNtKLVEEKYPISDVWNI 446
PTZ00176 PTZ00176
erythrocyte membrane protein 1 (PfEMP1); Provisional
2603-3053 5.85e-144

erythrocyte membrane protein 1 (PfEMP1); Provisional


Pssm-ID: 140204 [Multi-domain]  Cd Length: 1317  Bit Score: 487.24  E-value: 5.85e-144
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 2603 GIGFAT---FTYFYLKKKTKSTIDLLRVINIPKSDYDIPTKLSPNRYIPYTSGKYRGKRYIYLEGDSGTDS-GYTDHYSD 2678
Cdd:PTZ00176  894 GIGVALtlgLLLFKMRRKAKRQVDMIRILQMSQNEYGIPTTKSPNKYVPYGSQRYKGKTYLYVEGDTDEEKyMFMSDTTD 973
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 2679 ITSSSESEYEeLDINDIYVPDGPKYKTLIEVVLEPSgklsgntiptsdkntPSDTQNDIQNDDIPSSKITDNEWNTLKDE 2758
Cdd:PTZ00176  974 ITSSESEYEE-MDINDIYVPGSPKYKTLIEVVLEPS---------------KRDTQNDIPSDNTPSYKLTDEEWNQLKDD 1037
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 2759 FISQYLQSEQPNdipNDYTSGNSSTNTNITTMSRDNMEEKPFIMSIHDRNLYSGEEYNYNVNMSTNNVDIPINRDNNVYS 2838
Cdd:PTZ00176 1038 FISQYLPNTEPN---NNYRSGNSPTNTNNTTTSHDNMGEKPFIMSIHDRNLYTGEEISYNINMSTNTMDDPKYVSNNVYS 1114
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 2839 GIDLINDTLSGNKHIDIYDEVLKRKENELFGTKHPKHTNTHNVTK-PARDDPIHNQLNLFHKWLDRHRDMCEKWENHHER 2917
Cdd:PTZ00176 1115 GIDLINDTLSGNQHIDIYDEVLKRKENELFGTNHVKQTSIHSVAKnTYSDDAITNKINLFHKWLDRHRDMCEKWENHHER 1194
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 2918 LAKLKEKWENETHSGNThPSEsNKTLNTDVSIQIHMDNPKPINEFtyvdSNpnqvddtyVDSNPDNSSMDtiledlekyn 2997
Cdd:PTZ00176 1195 LAKLKEKWENDNDGGNV-PSD-NHVLNTDVSIEIDMDNPKPINQF----SN--------MDINVDTPTMD---------- 1250
                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 124015259 2998 epyyDVQDDIYYDVNDQDVS---------TVDSN--NMDVPSKVQIEMDI--NTK--LVKEKYPIADVWDI 3053
Cdd:PTZ00176 1251 ----NMEDDIYYDVNDNDDDndqpsvydiPMDHNkvDVDVPKKVHIEMKIlnNTSngSLEQQFPISDVWNI 1317
Duffy_binding pfam05424
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ...
116-301 2.97e-87

Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.


Pssm-ID: 428465 [Multi-domain]  Cd Length: 178  Bit Score: 282.62  E-value: 2.97e-87
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   116 ACAPYRRLHVCDQNLEQIEPIKITNTHNLLVDVCMAAKFEGQSITQDYPKYQATYGDSPSQICTMLARSFADIGDIVRGR 195
Cdd:pfam05424    1 ACAPPRRLHLCVKNLEKINSGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNTKYGDIPSEFCRQMAYSFADIGDIIRGK 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   196 DLYLGNPQEIKqrqqLENNLKTIFGKIYEKLNGAEaryGNDPEFFKLREDWWTANRETVWKAITCNAW-GNTYFHATCNR 274
Cdd:pfam05424   81 DLYLGNNKKKK----LEENLKKIFKKIYEKLTSKG---KDDGNYYQLREDWWEANREDIWKAMTCALTyGAKYFRKTCGD 153
                          170       180
                   ....*....|....*....|....*..
gi 124015259   275 GERTKGYCRcnDDQVPTYFDYVPQYLR 301
Cdd:pfam05424  154 GISPSTAGC--NDDVPTYFDYVPQFLR 178
PTZ00176 PTZ00176
erythrocyte membrane protein 1 (PfEMP1); Provisional
1-477 1.03e-73

erythrocyte membrane protein 1 (PfEMP1); Provisional


Pssm-ID: 140204 [Multi-domain]  Cd Length: 1317  Bit Score: 273.06  E-value: 1.03e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259    1 MGPKEaaGGDDIEDESAKHMFDRIGKDVYDKVKEEAKERGKgLQGRLSEAKFEKNESDP-----QTPEDPCDLDHKYHTN 75
Cdd:PTZ00176    1 MALKK--GVINESKLSARNVLENIGNEIKDKRENESKYTDK-LKGSLWEARFSDGLSSSfgdvrSGYYDSCSLDHKFHTN 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   76 VTTNVI---NPCADRSDVRFSDEYGGQCTHNRIKDSQQGDNKGACAPYRRLHVCDQNLEQIEPIKITNTHNLLVDVCMAA 152
Cdd:PTZ00176   78 INNGYPparNPCDGRNQNRFDENGESYCNSDKIRGNENNSNAGACAPFRRQNMCDKNLEYLINKNTENTHDLLGNVLVTA 157
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  153 KFEGQSITQDYPKYQAtygdspSQICTMLARSFADIGDIVRGRDLYLGNPQEikqrqQLENNLKTIFGKIYEKL--NGAE 230
Cdd:PTZ00176  158 KYEGESIVNNHPDKDK------SSVCTALARSFADIGDIVRGKDMFKRNKHD-----NVENGLREVFKKIYEGLknNGAR 226
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  231 ARYGNDPE--FFKLREDWWTANRETVWKAITCNAWGNTYFHATCNRGERTKG----YCRCNDDQVPTYFDYVPQYLRWFE 304
Cdd:PTZ00176  227 EHYKEVKNgnYIKLREDWWTANRDQVWKAMTCVAPENAYFRKTEADGIGISSlilpYSKCGRDTDPPVVDYIPQRLRWMS 306
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  305 EWAEDFCrkknkkikdvkrNCRGKDKEDKDRYCSrngyDCEKTKRAIGKLRyGKQCISCLYACNPYVDWINNQKEQFDKQ 384
Cdd:PTZ00176  307 EWSEYFC------------NVLNKEIDEMNNQCK----DCEMSRRCNNDTE-GEKCKKCKEQCQIFKELVSKWKNQFDKQ 369
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  385 KKKYDE-------EIKKYENGA--SGGSRQKRDAGGTTTTNYDGYEKKFYdELNKSEYRTVDKFLEKLSNeeiCTKVkde 455
Cdd:PTZ00176  370 SMKYMElynkastNITKQNSSApeRGYRRNHRRRGYDDDTNVQLFLKKVI-ENNECKVESLGKYLDKTSH---CGNY--- 442
                         490       500
                  ....*....|....*....|..
gi 124015259  456 eggtidfkNVNSDSTSGASGTN 477
Cdd:PTZ00176  443 --------NFNYDNTPGSNRSN 456
PTZ00176 PTZ00176
erythrocyte membrane protein 1 (PfEMP1); Provisional
879-1629 3.88e-64

erythrocyte membrane protein 1 (PfEMP1); Provisional


Pssm-ID: 140204 [Multi-domain]  Cd Length: 1317  Bit Score: 242.63  E-value: 3.88e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  879 LRGDISLAQFKNGRNGS-----TLKGQICKINEN-YSNDSRG--NSGGPCTGKDGDHGGVRMRIGTEWSNIEGKKQTSYK 950
Cdd:PTZ00176   40 LKGSLWEARFSDGLSSSfgdvrSGYYDSCSLDHKfHTNINNGypPARNPCDGRNQNRFDENGESYCNSDKIRGNENNSNA 119
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  951 NVFLPPRREHMCTSNLENLdvgsVTKNDKASHSLLGDVQLAAKTDAAEIIKRYKDqnniqltdpiqqKDQEAMCRAVRYS 1030
Cdd:PTZ00176  120 GACAPFRRQNMCDKNLEYL----INKNTENTHDLLGNVLVTAKYEGESIVNNHPD------------KDKSSVCTALARS 183
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1031 FADLGDIIRGRDMWDEDKSStDMETRLITVFKNIkekHDGIKDNP-KYTGDESKKPAYKKLRADWWEANRHQVWRAMKCA 1109
Cdd:PTZ00176  184 FADIGDIVRGKDMFKRNKHD-NVENGLREVFKKI---YEGLKNNGaREHYKEVKNGNYIKLREDWWTANRDQVWKAMTCV 259
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1110 T----------------KGIICP--------GMPVDDYIPQRLRWMTEWAEWYCKAQSQEYDKLKKISADC-MSKGDGKC 1164
Cdd:PTZ00176  260 ApenayfrkteadgigiSSLILPyskcgrdtDPPVVDYIPQRLRWMSEWSEYFCNVLNKEIDEMNNQCKDCeMSRRCNND 339
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1165 TQGDvDCGKCKAACDKYKEEIEKWNEQWRKISDKYNLLYLQAKT-----TSTNPGRTVLG-------DDDPDYQQMVDFL 1232
Cdd:PTZ00176  340 TEGE-KCKKCKEQCQIFKELVSKWKNQFDKQSMKYMELYNKASTnitkqNSSAPERGYRRnhrrrgyDDDTNVQLFLKKV 418
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1233 TPIHKASIAArvlvkraagspteiaaaapitpystaagyihqeigYGGCQEQTQFCeKKHGATSTSTTKENKEYTFKQPP 1312
Cdd:PTZ00176  419 IENNECKVES-----------------------------------LGKYLDKTSHC-GNYNFNYDNTPGSNRSNAFEITP 462
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1313 PEYATACDCINRSQTEE-PKKKEENVesaCKIVEKIlegkngrTTVGECNPKESYPDWDCK--NNIDISHDGACMPPRRQ 1389
Cdd:PTZ00176  463 EKFKKACKCKIPNPLEKcPNEENKNV---CTRFDKV-------YSCTSLSFKNDLSEWNNSgvKNKENDNNGVLVPPRRR 532
                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1390 KLCLYYIAHESQteNIKTDDNLKDAFIKTAAAETFLSWQYYkSKNDSEAkildrglipsqfLRSMMYTFGDYRDICLNTD 1469
Cdd:PTZ00176  533 NLCINLFSKKDY--KMKDENDFKEDLLNAAFSQGKLLGKKY-SNYSNEA------------YEAMKFSYADYSDIVKGTD 597
                         650       660       670       680       690       700       710       720
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1470 IpkkQNDVAKAKDKIGKFFsKDGSKSPSGLSRQEWWKTNGPEIWKGMLCAltkYVTDTDNKrkikndysydKVNQSQNGN 1549
Cdd:PTZ00176  598 M---MNDLKKLNKELNTLL-KETEKGDISVDRKTWWDDNKNVVWNAMLCG---YKTENENQ----------QLNSSWCNV 660
                         730       740       750       760       770       780       790       800
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1550 PSLEEFaakPQFLRWMIEWGEEFCAERQKKENIIKDACNEINSTQQCNDAKHRCNQACRA----YQEYVENKKKEFSGQT 1625
Cdd:PTZ00176  661 PDDDNI---DQFLRWLTEWAQQYCKEKLIKAHIINTKCKDIVEGRKHKSMVDITDVECKRlfidYEEWFRYRYNQWKGLS 737

                  ....
gi 124015259 1626 NNFV 1629
Cdd:PTZ00176  738 EKYI 741
Duffy_binding pfam05424
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ...
1382-1563 1.96e-46

Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.


Pssm-ID: 428465 [Multi-domain]  Cd Length: 178  Bit Score: 165.91  E-value: 1.96e-46
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  1382 ACMPPRRQKLCLYYIAHEsQTENIKTDDNLKDAFIKTAAAETFLSWQYYKSKNDSEakildrGLIPSQFLRSMMYTFGDY 1461
Cdd:pfam05424    1 ACAPPRRLHLCVKNLEKI-NSGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNTKY------GDIPSEFCRQMAYSFADI 73
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  1462 RDICLNTDIPKKQNDVAKAKDKIGKFFSKDGSKSPSGLS--------RQEWWKTNGPEIWKGMLCALTKyvtdtDNKRKI 1533
Cdd:pfam05424   74 GDIIRGKDLYLGNNKKKKLEENLKKIFKKIYEKLTSKGKddgnyyqlREDWWEANREDIWKAMTCALTY-----GAKYFR 148
                          170       180       190
                   ....*....|....*....|....*....|
gi 124015259  1534 KNDYSYDKVNQSQNGNPSLEEFAAKPQFLR 1563
Cdd:pfam05424  149 KTCGDGISPSTAGCNDDVPTYFDYVPQFLR 178
Duffy_binding pfam05424
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ...
955-1129 1.02e-43

Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.


Pssm-ID: 428465 [Multi-domain]  Cd Length: 178  Bit Score: 157.82  E-value: 1.02e-43
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   955 PPRREHMCTSNLENLDvgsvTKNDKASHSLLGDVQLAAKTDAAEIIKRYKDQNNIQLTDPIQqkdqeaMCRAVRYSFADL 1034
Cdd:pfam05424    4 PPRRLHLCVKNLEKIN----SGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNTKYGDIPSE------FCRQMAYSFADI 73
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  1035 GDIIRGRDMWDEDKSSTDMETRLITVFKNIKEKHdgikdnpkyTGDESKKPAYKKLRADWWEANRHQVWRAMKCA----- 1109
Cdd:pfam05424   74 GDIIRGKDLYLGNNKKKKLEENLKKIFKKIYEKL---------TSKGKDDGNYYQLREDWWEANREDIWKAMTCAltyga 144
                          170       180       190
                   ....*....|....*....|....*....|....
gi 124015259  1110 --------------TKGIICPGMPVDDYIPQRLR 1129
Cdd:pfam05424  145 kyfrktcgdgispsTAGCNDDVPTYFDYVPQFLR 178
Duffy_binding pfam05424
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ...
1782-2017 3.05e-41

Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.


Pssm-ID: 428465 [Multi-domain]  Cd Length: 178  Bit Score: 150.89  E-value: 3.05e-41
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  1782 ICIPPRRRRLYVGKLQEWATalpqgegaasPSHSRADDLRNAFIQSAAIETFFLWDRYKEEKKpqgdgsqqalsqltsty 1861
Cdd:pfam05424    1 ACAPPRRLHLCVKNLEKINS----------GNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNT----------------- 53
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  1862 sddeedppdkllQNGKIPTDFLRQMFYTLGDYRDIlVRGGSNTNGDTKDggdssnnnnivflasgkenkeamKKIQEQLK 1941
Cdd:pfam05424   54 ------------KYGDIPSEFCRQMAYSFADIGDI-IRGKDLYLGNNKK-----------------------KKLEENLK 97
                          170       180       190       200       210       220       230
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 124015259  1942 VFFSNSGNQPSTGGKNLSQSRDTPSSWWQKNGEHIWKGMICALTYkeNEEKTNDTQKIKKDEQVYEKIFGKPPNND 2017
Cdd:pfam05424   98 KIFKKIYEKLTSKGKDDGNYYQLREDWWEANREDIWKAMTCALTY--GAKYFRKTCGDGISPSTAGCNDDVPTYFD 171
PFEMP pfam03011
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ...
2333-2468 7.63e-36

PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.


Pssm-ID: 281064  Cd Length: 154  Bit Score: 134.55  E-value: 7.63e-36
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  2333 LVKRWVEYFFEDYNKIKHKISHRIKNGEISPCIK------NCVEKWVDQKRKEWKEITERFKDQYKNDNSDDD------N 2400
Cdd:pfam03011    1 LFKRWVEYFLEDSIKWRKKLKSCINNGKGKCCKKeckndcECFKKWVEKKKEEWKKIKEHFLKQYKLKGLTGKtldeylD 80
                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 124015259  2401 VRSFLETLIPQITDANAKNKV---IKLSKFGNSCGCSASANEQnKNGEYKDAIDCMLKKLKDKIGECEKKH 2468
Cdd:pfam03011   81 LKSFLETFLFYIDIKEAYGDVkelKKLEEILDEEGCSAGAESA-KNNKNEDAIDKLLDKEEKKANNCKEKH 150
PFEMP pfam03011
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ...
648-815 5.82e-30

PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.


Pssm-ID: 281064  Cd Length: 154  Bit Score: 117.60  E-value: 5.82e-30
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   648 FFYYWVAHMLKDSIHWKKKLQRCLQNGNRiKCGNNKCNNDCECFKRWITQKKDEWGKIVQHFKTQ-NIKGRGGSDntael 726
Cdd:pfam03011    1 LFKRWVEYFLEDSIKWRKKLKSCINNGKG-KCCKKECKNDCECFKKWVEKKKEEWKKIKEHFLKQyKLKGLTGKT----- 74
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259   727 ipfdhdyvlqynLQEEFLKGDSEDASEEKSENSLDAEEAEELKHLREIIESEDNNQEASVGGGvTEQKNIMDKLLNYEKD 806
Cdd:pfam03011   75 ------------LDEYLDLKSFLETFLFYIDIKEAYGDVKELKKLEEILDEEGCSAGAESAKN-NKNEDAIDKLLDKEEK 141

                   ....*....
gi 124015259   807 EADLCLEIH 815
Cdd:pfam03011  142 KANNCKEKH 150
PTZ00176 PTZ00176
erythrocyte membrane protein 1 (PfEMP1); Provisional
1364-1791 3.82e-15

erythrocyte membrane protein 1 (PfEMP1); Provisional


Pssm-ID: 140204 [Multi-domain]  Cd Length: 1317  Bit Score: 82.78  E-value: 3.82e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1364 ESYPDWD-CKNNIDISHDGACMPPRRQKLC---LYYIAHEsqteNIKTDDNLKDAFIKTAAAETFLSWQYYKSKNDSEAK 1439
Cdd:PTZ00176  102 ESYCNSDkIRGNENNSNAGACAPFRRQNMCdknLEYLINK----NTENTHDLLGNVLVTAKYEGESIVNNHPDKDKSSVC 177
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1440 IldrglipsqflrSMMYTFGDYRDICLNTDI--PKKQNDVAKAK----DKIGKFFSKDGSKS-----PSG---LSRQEWW 1505
Cdd:PTZ00176  178 T------------ALARSFADIGDIVRGKDMfkRNKHDNVENGLrevfKKIYEGLKNNGAREhykevKNGnyiKLREDWW 245
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1506 KTNGPEIWKGMLCALTKYV----TDTDNKRKIKNDYSYDKVnqSQNGNPSLEEFAakPQFLRWMIEWGEEFCAERQKKEN 1581
Cdd:PTZ00176  246 TANRDQVWKAMTCVAPENAyfrkTEADGIGISSLILPYSKC--GRDTDPPVVDYI--PQRLRWMSEWSEYFCNVLNKEID 321
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1582 IIKDACNEINSTQQCNDAKH-----RCNQACRAYQEYVENKKKEFSGQTNNF----------VLKANVQPQDPEYKGYEY 1646
Cdd:PTZ00176  322 EMNNQCKDCEMSRRCNNDTEgekckKCKEQCQIFKELVSKWKNQFDKQSMKYmelynkastnITKQNSSAPERGYRRNHR 401
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259 1647 KDGVQPIQGNEYLLQKC-DNNKCscmdgnvlsvspKEKPFGKYAHKyPEKCDCYQGKHVPSIPPPPPPVQPQPEAPTVTV 1725
Cdd:PTZ00176  402 RRGYDDDTNVQLFLKKViENNEC------------KVESLGKYLDK-TSHCGNYNFNYDNTPGSNRSNAFEITPEKFKKA 468
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 124015259 1726 DVCSIVKTLFKDTNNFSDACGLKYGKTAPSSWKCIPSDAKSGAGATTGKSGSDSGSICIPPRRRRL 1791
Cdd:PTZ00176  469 CKCKIPNPLEKCPNEENKNVCTRFDKVYSCTSLSFKNDLSEWNNSGVKNKENDNNGVLVPPRRRNL 534
CIDR1_gamma pfam18562
Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind ...
2264-2317 9.28e-15

Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind uninfected RBCs, which is consistently associated with severe malaria in African children. The rosette-forming PfEMP1 adhesins, namely IT4/R29, Palo Alto 89F5 VarO, 3D7/PF13_0003 and IT4/var60, belong to a specific sub-group called groupA/UpsA var genes and all four present a specific Duffy Binding-Like and and Cysteine-Rich Interdomain Region (DBL1alpha1-CIDR1gamma) double domain Head region found at the extracellular region of PfEMP1. This entry represents the CIDR1gamma domain which increases the binding affinity to VarO (Palo Alto VarO parasites).


Pssm-ID: 408346  Cd Length: 52  Bit Score: 70.76  E-value: 9.28e-15
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....
gi 124015259  2264 STVLEMRVSADSKSGFnGDGLENaCRGAGIFEGIRKDEWKCRNVCGYVVCKPEN 2317
Cdd:pfam18562    1 TTTIDMLVSDNRGIKF-ENDLKE-CKEAGIFKGIRKDQWKCGKVCGYDVCKLKN 52
NTS pfam15447
N-terminal segments of PfEMP1; This family, the N-terminal segment, is the most variable part ...
16-51 4.79e-11

N-terminal segments of PfEMP1; This family, the N-terminal segment, is the most variable part of the variant surface antigen family of Plasmodium falciparum, the erythrocyte membrane protein-1 (PfEMP1) proteins. PfEMP1 is an important target for protective immunity and is implicated in the pathology of malaria through its ability to adhere to host endothelial receptors. A structural and functional study of the N-terminal domain of PfEMP1 from the VarO variant comprising the N-terminal segment (NTS) and the first DBL domain (DBL1alpha1), shows this region is directly implicated in rosetting. NTS, previously thought to be a structurally independent component of PfEMP1, forms an integral part of the DBL1alpha domain that is found to be the important heparin-binding site. This family is closely associated with PFEMP, pfam03011, and Duffy_binding, pfam05424.


Pssm-ID: 406013 [Multi-domain]  Cd Length: 36  Bit Score: 59.72  E-value: 4.79e-11
                           10        20        30
                   ....*....|....*....|....*....|....*.
gi 124015259    16 SAKHMFDRIGKDVYDKVKEEAKERGKGLQGRLSEAK 51
Cdd:pfam15447    1 SAKHLLDRIGKDVHDKVKKEAKRYKSELKGDLSKAK 36
PTZ00176 PTZ00176
erythrocyte membrane protein 1 (PfEMP1); Provisional
108-312 5.19e-10

erythrocyte membrane protein 1 (PfEMP1); Provisional


Pssm-ID: 140204 [Multi-domain]  Cd Length: 1317  Bit Score: 65.83  E-value: 5.19e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  108 SQQGDNKGACAPYRRLHVCdQNLEQIEPIKITNTHNLLVDVCMAAKFEGQSITQDYPKYQatygdspSQICTMLARSFAD 187
Cdd:PTZ00176  517 NKENDNNGVLVPPRRRNLC-INLFSKKDYKMKDENDFKEDLLNAAFSQGKLLGKKYSNYS-------NEAYEAMKFSYAD 588
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124015259  188 IGDIVRGRDLYlgnpqeikqrqqleNNLKtifgKIYEKLNG--AEARYGndpEFFKLREDWWTANRETVWKAITCNawgn 265
Cdd:PTZ00176  589 YSDIVKGTDMM--------------NDLK----KLNKELNTllKETEKG---DISVDRKTWWDDNKNVVWNAMLCG---- 643
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 124015259  266 tyfHATCNRGER-TKGYCRCNDDqvptyfDYVPQYLRWFEEWAEDFCR 312
Cdd:PTZ00176  644 ---YKTENENQQlNSSWCNVPDD------DNIDQFLRWLTEWAQQYCK 682
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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