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Conserved domains on  [gi|123145780|sp|Q0SP20|]
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RecName: Full=Putative membrane protein insertion efficiency factor

Protein Classification

membrane protein insertion efficiency factor YidD( domain architecture ID 10014664)

membrane protein insertion efficiency factor YidD may be involved in the insertion of integral membrane proteins into the membrane

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK14385 PRK14385
membrane protein insertion efficiency factor YidD;
1-96 2.31e-44

membrane protein insertion efficiency factor YidD;


:

Pssm-ID: 172861  Cd Length: 96  Bit Score: 138.41  E-value: 2.31e-44
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 123145780  1 MNIFKIFFILNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPKY 80
Cdd:PRK14385  1 MIIKKIFIILNLILILLIKIYQNTLSKIVGFQCIYKPSCSNYALECLKKYNILTALILITLRIIRCNALFKGGNESLPTK 80

                        90
                ....*....|....*.
gi 123145780 81 NPISTSLKEFQKRLIK 96
Cdd:PRK14385 81 KPILNSLKEFKTRLIK 96
 
Name Accession Description Interval E-value
PRK14385 PRK14385
membrane protein insertion efficiency factor YidD;
1-96 2.31e-44

membrane protein insertion efficiency factor YidD;


Pssm-ID: 172861  Cd Length: 96  Bit Score: 138.41  E-value: 2.31e-44
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 123145780  1 MNIFKIFFILNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPKY 80
Cdd:PRK14385  1 MIIKKIFIILNLILILLIKIYQNTLSKIVGFQCIYKPSCSNYALECLKKYNILTALILITLRIIRCNALFKGGNESLPTK 80

                        90
                ....*....|....*.
gi 123145780 81 NPISTSLKEFQKRLIK 96
Cdd:PRK14385 81 KPILNSLKEFKTRLIK 96
TIGR00278 TIGR00278
putative membrane protein insertion efficiency factor; This model describes a family, YidD, of ...
 14-88 1.21e-29

putative membrane protein insertion efficiency factor; This model describes a family, YidD, of small, non-essential proteins now suggested to improve YidC-dependent inner membrane protein insertion. A related protein is found in the temperature phage HP1 of Haemophilus influenzae. Annotation of some members of this family as hemolysins appears to represent propagation from an unpublished GenBank submission, L36462, attributed to Aeromonas hydrophila but a close match to E. coli. [Hypothetical proteins, Conserved]


Pssm-ID: 272995  Cd Length: 75  Bit Score: 100.61  E-value: 1.21e-29
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 123145780  14 IIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPKYNPISTSLK 88
Cdd:TIGR00278  1 LIALIRGYQIFISPFFPPSCRFYPTCSQYAIEALKTHGFLKGGWLTLKRILRCHPLHPGGNDPVPKKKQILESLK 75
YidD COG0759
Membrane-anchored protein YidD, putatitve component of membrane protein insertase Oxa1/YidC ...
 9-75 4.85e-21

Membrane-anchored protein YidD, putatitve component of membrane protein insertase Oxa1/YidC/SpoIIIJ [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440522  Cd Length: 71  Bit Score: 78.62  E-value: 4.85e-21
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 123145780  9 ILNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGND 75
Cdd:COG0759   3 LMKRLLIGLIRFYQRFISPLLGPRCRFYPTCSEYALEAIERHGLLKGSWLTLKRILRCHPWHPGGYD 69
YidD pfam01809
Putative membrane protein insertion efficiency factor; This family consists of membrane ...
 14-75 8.60e-20

Putative membrane protein insertion efficiency factor; This family consists of membrane insertion efficiency factor proteins. They contain three conserved cysteine residues. Family members such as YidD may be involved in insertion of integral membrane proteins into the membrane by assisting YidC (membrane protein insertase). Some members of the yidD family have been previously thought to posses alpha-hemolysin activity, however no sufficient evidence was found to corroborate this idea. Secondary structure prediction indicated the presence of three alpha-helices in YidD. None of the three alpha-helices appeared sufficiently hydrophobic to serve as a transmembrane, suggesting a cytoplasmic localization for YidD. However, a closer examination of the alpha-helical wheel projection of the predicted first alpha-helix in YidD suggested an amphipathic structure in its N-terminal region which might be involved in membrane targeting.


Pssm-ID: 460342  Cd Length: 67  Bit Score: 75.49  E-value: 8.60e-20
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 123145780  14 IIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGND 75
Cdd:pfam01809  4 LIGLIRFYQRFISPLLPPSCRFYPTCSEYAIEAIEKHGALKGGWLALKRILRCHPFHPGGYD 65
 
Name Accession Description Interval E-value
PRK14385 PRK14385
membrane protein insertion efficiency factor YidD;
1-96 2.31e-44

membrane protein insertion efficiency factor YidD;


Pssm-ID: 172861  Cd Length: 96  Bit Score: 138.41  E-value: 2.31e-44
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 123145780  1 MNIFKIFFILNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPKY 80
Cdd:PRK14385  1 MIIKKIFIILNLILILLIKIYQNTLSKIVGFQCIYKPSCSNYALECLKKYNILTALILITLRIIRCNALFKGGNESLPTK 80

                        90
                ....*....|....*.
gi 123145780 81 NPISTSLKEFQKRLIK 96
Cdd:PRK14385 81 KPILNSLKEFKTRLIK 96
TIGR00278 TIGR00278
putative membrane protein insertion efficiency factor; This model describes a family, YidD, of ...
 14-88 1.21e-29

putative membrane protein insertion efficiency factor; This model describes a family, YidD, of small, non-essential proteins now suggested to improve YidC-dependent inner membrane protein insertion. A related protein is found in the temperature phage HP1 of Haemophilus influenzae. Annotation of some members of this family as hemolysins appears to represent propagation from an unpublished GenBank submission, L36462, attributed to Aeromonas hydrophila but a close match to E. coli. [Hypothetical proteins, Conserved]


Pssm-ID: 272995  Cd Length: 75  Bit Score: 100.61  E-value: 1.21e-29
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 123145780  14 IIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPKYNPISTSLK 88
Cdd:TIGR00278  1 LIALIRGYQIFISPFFPPSCRFYPTCSQYAIEALKTHGFLKGGWLTLKRILRCHPLHPGGNDPVPKKKQILESLK 75
YidD COG0759
Membrane-anchored protein YidD, putatitve component of membrane protein insertase Oxa1/YidC ...
 9-75 4.85e-21

Membrane-anchored protein YidD, putatitve component of membrane protein insertase Oxa1/YidC/SpoIIIJ [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440522  Cd Length: 71  Bit Score: 78.62  E-value: 4.85e-21
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 123145780  9 ILNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGND 75
Cdd:COG0759   3 LMKRLLIGLIRFYQRFISPLLGPRCRFYPTCSEYALEAIERHGLLKGSWLTLKRILRCHPWHPGGYD 69
YidD pfam01809
Putative membrane protein insertion efficiency factor; This family consists of membrane ...
 14-75 8.60e-20

Putative membrane protein insertion efficiency factor; This family consists of membrane insertion efficiency factor proteins. They contain three conserved cysteine residues. Family members such as YidD may be involved in insertion of integral membrane proteins into the membrane by assisting YidC (membrane protein insertase). Some members of the yidD family have been previously thought to posses alpha-hemolysin activity, however no sufficient evidence was found to corroborate this idea. Secondary structure prediction indicated the presence of three alpha-helices in YidD. None of the three alpha-helices appeared sufficiently hydrophobic to serve as a transmembrane, suggesting a cytoplasmic localization for YidD. However, a closer examination of the alpha-helical wheel projection of the predicted first alpha-helix in YidD suggested an amphipathic structure in its N-terminal region which might be involved in membrane targeting.


Pssm-ID: 460342  Cd Length: 67  Bit Score: 75.49  E-value: 8.60e-20
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 123145780  14 IIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGND 75
Cdd:pfam01809  4 LIGLIRFYQRFISPLLPPSCRFYPTCSEYAIEAIEKHGALKGGWLALKRILRCHPFHPGGYD 65
PRK14379 PRK14379
membrane protein insertion efficiency factor YidD;
10-80 3.03e-14

membrane protein insertion efficiency factor YidD;


Pssm-ID: 237697  Cd Length: 95  Bit Score: 62.15  E-value: 3.03e-14
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 123145780 10 LNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPKY 80
Cdd:PRK14379  1 MRKILIFLVRVYQKFISPILPPSCRYYPTCSSYMIDALKKHGPILGLIMGLARILRCNPFIRGGVDPVPDY 71
PRK14391 PRK14391
membrane protein insertion efficiency factor YidD;
10-78 4.04e-13

membrane protein insertion efficiency factor YidD;


Pssm-ID: 172867  Cd Length: 84  Bit Score: 59.27  E-value: 4.04e-13
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 123145780 10 LNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIP 78
Cdd:PRK14391  1 MNQFVIQLIQLYKKIISPLLPPACRFTPTCSEYAAQAFQECGFFQAIQLSAWRILRCNPLSQGFDDPLP 69
PRK14369 PRK14369
membrane protein insertion efficiency factor YidD;
10-78 7.47e-13

membrane protein insertion efficiency factor YidD;


Pssm-ID: 184650  Cd Length: 119  Bit Score: 59.30  E-value: 7.47e-13
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 123145780  10 LNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIP 78
Cdd:PRK14369   1 MKYPLLALIKLYQWTISPLLGPVCKYYPSCSHYGYTAIDRHGAIKGTALTAWRILRCNPWSLGGVDHVP 69
PRK14382 PRK14382
hypothetical protein; Provisional
 10-76 2.13e-12

hypothetical protein; Provisional


Pssm-ID: 172858  Cd Length: 68  Bit Score: 56.70  E-value: 2.13e-12
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 123145780 10 LNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDF 76
Cdd:PRK14382  1 MKRLLIKFIKFYQKFISPLYPSSCRFYPTCSTYAILSIEKYGALKGSLKAFWRILRCNPFSKGGVDY 67
PRK14372 PRK14372
membrane protein insertion efficiency factor YidD;
14-79 6.21e-11

membrane protein insertion efficiency factor YidD;


Pssm-ID: 172848  Cd Length: 97  Bit Score: 53.79  E-value: 6.21e-11
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 123145780 14 IIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPK 79
Cdd:PRK14372  1 MIRGIRWYQQRISANTPPCCKYYPSCSNYAIEAIERFGAFKGGVLAVLRLLRCRPWSRGGIDDVPQ 66
PRK14373 PRK14373
hypothetical protein; Provisional
 8-78 8.98e-11

hypothetical protein; Provisional


Pssm-ID: 172849  Cd Length: 73  Bit Score: 52.70  E-value: 8.98e-11
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 123145780  8 FILNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIP 78
Cdd:PRK14373  3 FLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKKYGAFKGGRLAIKRIARCHPFGGHGHDPVP 73
PRK14383 PRK14383
membrane protein insertion efficiency factor YidD;
14-82 1.31e-10

membrane protein insertion efficiency factor YidD;


Pssm-ID: 237698 [Multi-domain]  Cd Length: 84  Bit Score: 52.65  E-value: 1.31e-10
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 123145780 14 IIFLIKIYQNTFS-KLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPKYNP 82
Cdd:PRK14383  8 LVRAVRWYQRVLSpRKPAPTCRFSPTCSQYAVEAIERHGALKGGWLALWRVLRCNPLVPGGFDPVPEHFP 77
PRK14381 PRK14381
membrane protein insertion efficiency factor YidD;
17-89 4.11e-10

membrane protein insertion efficiency factor YidD;


Pssm-ID: 172857  Cd Length: 103  Bit Score: 51.78  E-value: 4.11e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 123145780  17 LIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPKynpisTSLKE 89
Cdd:PRK14381  18 LIHLYRWTISPLLGSPCRFFPSCSQYALQALKHHGCIMGLWLTIKRIGKCGPWHPGGIDLVPM-----TTLEE 85
PRK14386 PRK14386
membrane protein insertion efficiency factor YidD;
16-78 1.04e-09

membrane protein insertion efficiency factor YidD;


Pssm-ID: 172862  Cd Length: 106  Bit Score: 50.80  E-value: 1.04e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 123145780  16 FLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIP 78
Cdd:PRK14386   7 WFIRLYQIAISPLLGPRCRYIPTCSQYALEALQTHGAIKGVWLSSKRICRCHPWGGSGYDPVP 69
PRK14387 PRK14387
membrane protein insertion efficiency factor YidD;
18-78 2.99e-09

membrane protein insertion efficiency factor YidD;


Pssm-ID: 184655  Cd Length: 84  Bit Score: 49.17  E-value: 2.99e-09
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 123145780 18 IKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIP 78
Cdd:PRK14387 12 LRAYKLTLSPLIGRQCRFTPSCSEYTAAALKDHGPLKGSWLGLRRICRCNPFGGSGYDPPP 72
PRK14380 PRK14380
hypothetical protein; Provisional
 5-75 9.03e-09

hypothetical protein; Provisional


Pssm-ID: 184654  Cd Length: 81  Bit Score: 48.02  E-value: 9.03e-09
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 123145780  5 KIFFILNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGND 75
Cdd:PRK14380  3 KINLMLRQIVCLPIKMYQYFISPLITPCCRYYPSCSEYADSAIKHYGVIKGLLMALNRLSRCHPWSKGGYD 73
PRK14389 PRK14389
membrane protein insertion efficiency factor YidD;
14-87 1.26e-08

membrane protein insertion efficiency factor YidD;


Pssm-ID: 184656  Cd Length: 98  Bit Score: 48.01  E-value: 1.26e-08
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 123145780 14 IIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPKYNPISTSL 87
Cdd:PRK14389  6 LMALVRGYRLLLSPWLGSACRFEPTCSAYSLQALEQHGAAAGSYLTLRRLARCHPWCDGGHDPVPQEKPRGMRL 79
PRK14371 PRK14371
hypothetical protein; Provisional
 18-86 7.88e-07

hypothetical protein; Provisional


Pssm-ID: 172847  Cd Length: 81  Bit Score: 42.99  E-value: 7.88e-07
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 123145780 18 IKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPkynPISTS 86
Cdd:PRK14371  9 IQFYRYAISPLMASHCRFYPSCSCYAYEAIENHGLLRGGWLSVRRLGRCHPWNPGGYDPVP---PAPTS 74
PRK14388 PRK14388
membrane protein insertion efficiency factor YidD;
14-78 8.99e-07

membrane protein insertion efficiency factor YidD;


Pssm-ID: 172864  Cd Length: 82  Bit Score: 42.96  E-value: 8.99e-07
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 123145780 14 IIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIP 78
Cdd:PRK14388  5 LLLLLRFYQYFISPLLGNNCRFHPTCSEYAKEAISMHGSIKGLWFTFKRIIKCQPFCDGGYDTVP 69
PRK14375 PRK14375
membrane protein insertion efficiency factor YidD;
17-71 2.04e-06

membrane protein insertion efficiency factor YidD;


Pssm-ID: 172851  Cd Length: 70  Bit Score: 41.51  E-value: 2.04e-06
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*
gi 123145780 17 LIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFK 71
Cdd:PRK14375  2 LINLYRYCISPFIPARCRYYPTCSEYALEALKTHGILKGLYLTTRRLLRCHPLSK 56
PRK14370 PRK14370
hypothetical protein; Provisional
 14-78 4.49e-06

hypothetical protein; Provisional


Pssm-ID: 184651  Cd Length: 120  Bit Score: 41.76  E-value: 4.49e-06
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 123145780  14 IIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIP 78
Cdd:PRK14370  13 LLAFIHFYQRIISPILPARCRYYPTCSNYGKQAIAWHGVGKGSLLLLKRIGRCHPLGGHGIDFVP 77
PRK14376 PRK14376
membrane protein insertion efficiency factor YidD;
10-82 5.90e-06

membrane protein insertion efficiency factor YidD;


Pssm-ID: 237695  Cd Length: 176  Bit Score: 42.25  E-value: 5.90e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 123145780  10 LNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPKYNP 82
Cdd:PRK14376  41 LAWTFAGLVRLYRAGWSGRNAGCCRFEPSCSAYALTAVRRHGGVRGGLLAVRRLLRCQPLSAGGYDPVPGEAP 113
PRK14384 PRK14384
hypothetical protein; Provisional
 33-79 1.25e-05

hypothetical protein; Provisional


Pssm-ID: 172860  Cd Length: 56  Bit Score: 39.27  E-value: 1.25e-05
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*..
gi 123145780 33 CIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGGNDFIPK 79
Cdd:PRK14384  4 CRFYPSCSCYAETALKRFGVIKGSGLTVWRLLRCHPFHPGGVDFVPE 50
PRK14374 PRK14374
membrane protein insertion efficiency factor YidD;
10-73 9.42e-05

membrane protein insertion efficiency factor YidD;


Pssm-ID: 237694  Cd Length: 118  Bit Score: 38.13  E-value: 9.42e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 123145780  10 LNYTIIFLIKIYQNTFSKLFGQQCIYKPTCSKYSIECLKKYNFLTALVLMTLRTIRCNALFKGG 73
Cdd:PRK14374   9 LSAIFTASIRGYQRFFSAFTFSSCRFYPTCSNYALWLLCFENPLSAMGKIAIRILSCNPFCSGG 72
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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