|
Name |
Accession |
Description |
Interval |
E-value |
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
6-649 |
0e+00 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 1368.68 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 6 KYPIPPAIAENALINPAQYHDFYQQSVQNPDAFWGEQGKIIDWIKPYsivkNTSFDPGHVSIRWFEDGTLNLSANCLDRH 85
Cdd:PRK00174 1 VFPPPAEFAANALIDMEQYKALYQESVEDPEGFWAEQAKRLDWFKPF----DTVLDWNAPFIKWFEDGELNVSYNCLDRH 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 86 LTERGEQTAIIWEGDDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGG 165
Cdd:PRK00174 77 LKTRGDKVAIIWEGDDPGDSRKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVVFGG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 166 FSPDAVAGRIIDSNAKLVITADEGMRAGRAIPLKKNVDDALKNPAitSINNVVVFQRTGKTGYWQEGRDLWWHDLIVGVS 245
Cdd:PRK00174 157 FSAEALADRIIDAGAKLVITADEGVRGGKPIPLKANVDEALANCP--SVEKVIVVRRTGGDVDWVEGRDLWWHELVAGAS 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 246 ADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGA 325
Cdd:PRK00174 235 DECEPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAAMTMKYVFDYKDGDVYWCTADVGWVTGHSYIVYGPLANGA 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 326 MTLMFEGVPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNG 405
Cdd:PRK00174 315 TTLMFEGVPNYPDPGRFWEVIDKHKVTIFYTAPTAIRALMKEGDEHPKKYDLSSLRLLGSVGEPINPEAWEWYYKVVGGE 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 406 QCPIVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSWPGQARTLYGDHDRFE 485
Cdd:PRK00174 395 RCPIVDTWWQTETGGIMITPLPGATPLKPGSATRPLPGIQPAVVDEEGNPLEGGEGGNLVIKDPWPGMMRTIYGDHERFV 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 486 QTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITL 565
Cdd:PRK00174 475 KTYFSTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIKGQGIYAFVTL 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 566 NHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGDTsNLGDTSTLADPSVVEKLLEEK 645
Cdd:PRK00174 555 KGGEEPSDELRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGKIMRRILRKIAEGEE-ILGDTSTLADPSVVEKLIEAR 633
|
....
gi 1206237686 646 QSMQ 649
Cdd:PRK00174 634 QNRK 637
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
18-644 |
0e+00 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 1190.90 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 18 LINPAQYHDFYQQSVQNPDAFWGEQGK-IIDWIKPYSIVKNTSFDPghvSIRWFEDGTLNLSANCLDRHLTERGEQTAII 96
Cdd:TIGR02188 1 IANLEQYKELYEESIEDPDKFWAKLAReLLDWFKPFTKVLDWSFPP---FYKWFVGGELNVSYNCVDRHLEARPDKVAII 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 97 WEGDDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRII 176
Cdd:TIGR02188 78 WEGDEPGEVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVFGGFSAEALADRIN 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 177 DSNAKLVITADEGMRAGRAIPLKKNVDDALKNPAiTSINNVVVFQRTG-KTGYWQEGRDLWWHDLIVGVSADCPAEEMNA 255
Cdd:TIGR02188 158 DAGAKLVITADEGLRGGKVIPLKAIVDEALEKCP-VSVEHVLVVRRTGnPVVPWVEGRDVWWHDLMAKASAYCEPEPMDS 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 256 EDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVPN 335
Cdd:TIGR02188 237 EDPLFILYTSGSTGKPKGVLHTTGGYLLYAAMTMKYVFDIKDGDIFWCTADVGWITGHSYIVYGPLANGATTVMFEGVPT 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 336 YPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNGQCPIVDTWWQ 415
Cdd:TIGR02188 317 YPDPGRFWEIIEKHKVTIFYTAPTAIRALMRLGDEWVKKHDLSSLRLLGSVGEPINPEAWMWYYKVVGKERCPIVDTWWQ 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 416 TETGGFMITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGN-LVITDSWPGQARTLYGDHDRFEQTYFSTFKG 494
Cdd:TIGR02188 397 TETGGIMITPLPGATPTKPGSATLPFFGIEPAVVDEEGNPVEGPGEGGyLVIKQPWPGMLRTIYGDHERFVDTYFSPFPG 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 495 MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPE 574
Cdd:TIGR02188 477 YYFTGDGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTLKDGYEPDDE 556
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 575 LYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGDTSNLGDTSTLADPSVVEKLLEE 644
Cdd:TIGR02188 557 LRKELRKHVRKEIGPIAKPDKIRFVPGLPKTRSGKIMRRLLRKIAAGEAEILGDTSTLEDPSVVEELIEA 626
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
23-634 |
0e+00 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 1173.49 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 23 QYHDFYQQSVQNPDAFWGEQGKIIDWIKPYSIVKNTSFDPGHvsIRWFEDGTLNLSANCLDRHLTERGEQTAIIWEGDDP 102
Cdd:cd05966 2 QYKELYKQSIEDPEEFWGEIAKELDWFKPWDKVLDWSKGPPF--IKWFEGGKLNISYNCLDRHLKERGDKVAIIWEGDEP 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 103 SQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKL 182
Cdd:cd05966 80 DQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGFSAESLADRINDAQCKL 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 183 VITADEGMRAGRAIPLKKNVDDALKNpaITSINNVVVFQRTGKTGYWQEGRDLWWHDLIVGVSADCPAEEMNAEDPLFIL 262
Cdd:cd05966 160 VITADGGYRGGKVIPLKEIVDEALEK--CPSVEKVLVVKRTGGEVPMTEGRDLWWHDLMAKQSPECEPEWMDSEDPLFIL 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 263 YTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNYPGVNRL 342
Cdd:cd05966 238 YTSGSTGKPKGVVHTTGGYLLYAATTFKYVFDYHPDDIYWCTADIGWITGHSYIVYGPLANGATTVMFEGTPTYPDPGRY 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 343 GQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNGQCPIVDTWWQTETGGFM 422
Cdd:cd05966 318 WDIVEKHKVTIFYTAPTAIRALMKFGDEWVKKHDLSSLRVLGSVGEPINPEAWMWYYEVIGKERCPIVDTWWQTETGGIM 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 423 ITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSWPGQARTLYGDHDRFEQTYFSTFKGMYFSGDGA 502
Cdd:cd05966 398 ITPLPGATPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPWPGMARTIYGDHERYEDTYFSKFPGYYFTGDGA 477
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 503 RRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNW 582
Cdd:cd05966 478 RRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEPSDELRKELRKH 557
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|..
gi 1206237686 583 VRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGdTSNLGDTSTLAD 634
Cdd:cd05966 558 VRKEIGPIATPDKIQFVPGLPKTRSGKIMRRILRKIAAG-EEELGDTSTLAD 608
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
24-611 |
0e+00 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 979.75 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 24 YHDFYQQSVQNPDAFWGEQGKIIDWIKPYSIVKNTSFDPGHVSIRWFEDGTLNLSANCLDRHLTERGEQTAIIWEGDDPS 103
Cdd:cd17634 1 YETKYRQSINDPDTFWGEAGKILDWITPYQKVKNTSFAPGAPSIKWFEDATLNLAANALDRHLRENGDRTAIIYEGDDTS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 104 QSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLV 183
Cdd:cd17634 81 QSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLL 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 184 ITADEGMRAGRAIPLKKNVDDALkNPAITSINNVVVFQRTGKTGYWQEGRDLWWHDLIVGVSADCPAEEMNAEDPLFILY 263
Cdd:cd17634 161 ITADGGVRAGRSVPLKKNVDDAL-NPNVTSVEHVIVLKRTGSDIDWQEGRDLWWRDLIAKASPEHQPEAMNAEDPLFILY 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 264 TSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNYPGVNRLG 343
Cdd:cd17634 240 TSGTTGKPKGVLHTTGGYLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHSYLLYGPLACGATTLLYEGVPNWPTPARMW 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 344 QVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNGQCPIVDTWWQTETGGFMI 423
Cdd:cd17634 320 QVVDKHGVNILYTAPTAIRALMAAGDDAIEGTDRSSLRILGSVGEPINPEAYEWYWKKIGKEKCPVVDTWWQTETGGFMI 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 424 TPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSWPGQARTLYGDHDRFEQTYFSTFKGMYFSGDGAR 503
Cdd:cd17634 400 TPLPGAIELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDPWPGQTRTLFGDHERFEQTYFSTFKGMYFSGDGAR 479
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 504 RDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWV 583
Cdd:cd17634 480 RDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSPELYAELRNWV 559
|
570 580
....*....|....*....|....*...
gi 1206237686 584 RKEIGPIATPDILHWTDSLPKTRSGKIM 611
Cdd:cd17634 560 RKEIGPLATPDVVHWVDSLPKTRSGKIM 587
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
68-643 |
0e+00 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 941.46 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 68 RWFEDGTLNLSANCLDRHLTERGEQTAIIWEGDDpSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAV 147
Cdd:COG0365 1 RWFVGGRLNIAYNCLDRHAEGRGDKVALIWEGED-GEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 148 AMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADEGMRAGRAIPLKKNVDDALKnpAITSINNVVVFQRTGKTG 227
Cdd:COG0365 80 AMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLRGGKVIDLKEKVDEALE--ELPSLEHVIVVGRTGADV 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 228 YWQEgrDLWWHDLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADV 307
Cdd:COG0365 158 PMEG--DLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDVFWCTADI 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 308 GWVTGHSYLLYGPLACGAMTLMFEGVPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVG 387
Cdd:COG0365 236 GWATGHSYIVYGPLLNGATVVLYEGRPDFPDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDEPLKKYDLSSLRLLGSAG 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 388 EPINPEAWEWYYNKIGngqCPIVDTWWQTETGGFMITPLPGaTELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVIT 467
Cdd:COG0365 316 EPLNPEVWEWWYEAVG---VPIVDGWGQTETGGIFISNLPG-LPVKPGSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIK 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 468 DSWPGQARTLYGDHDRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAV 547
Cdd:COG0365 392 GPWPGMFRGYWNDPERYRETYFGRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAV 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 548 VGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGDtsNLG 627
Cdd:COG0365 472 VGVPDEIRGQVVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIAEGR--PLG 549
|
570
....*....|....*.
gi 1206237686 628 DTSTLADPSVVEKLLE 643
Cdd:COG0365 550 DTSTLEDPEALDEIKE 565
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
14-643 |
0e+00 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 841.87 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 14 AENALI-NPAQYHDFYQQSVQNPDAFWGEQGKIIDWIKPYS----IVKNTSFDPGHVSIRWFEDGTLNLSANCLDRHLTE 88
Cdd:PLN02654 21 SAQALVsSPQQYMEMYKRSVDDPAGFWSDIASQFYWKQKWEgdevCSENLDVRKGPISIEWFKGGKTNICYNCLDRNVEA 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 89 -RGEQTAIIWEGDDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFS 167
Cdd:PLN02654 101 gNGDKIAIYWEGNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFS 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 168 PDAVAGRIIDSNAKLVITADEGMRAGRAIPLKKNVDDALKNPAITSIN--------NVVVFQRTGKTgyWQEGRDLWWHD 239
Cdd:PLN02654 181 AESLAQRIVDCKPKVVITCNAVKRGPKTINLKDIVDAALDESAKNGVSvgicltyeNQLAMKREDTK--WQEGRDVWWQD 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 240 LIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYG 319
Cdd:PLN02654 259 VVPNYPTKCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVYWCTADCGWITGHSYVTYG 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 320 PLACGAMTLMFEGVPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYY 399
Cdd:PLN02654 339 PMLNGATVLVFEGAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYVTRHSRKSLRVLGSVGEPINPSAWRWFF 418
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 400 NKIGNGQCPIVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSWPGQARTLYG 479
Cdd:PLN02654 419 NVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGKEIEGECSGYLCVKKSWPGAFRTLYG 498
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 480 DHDRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAI 559
Cdd:PLN02654 499 DHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGI 578
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 560 YAYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGDTSNLGDTSTLADPSVVE 639
Cdd:PLN02654 579 YAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASRQLDELGDTSTLADPGVVD 658
|
....
gi 1206237686 640 KLLE 643
Cdd:PLN02654 659 QLIA 662
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
24-638 |
0e+00 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 657.08 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 24 YHDFYQQSVQNPDAFWGEQGKIIDWIKPYSIVKNTSFDPGHvsiRWFEDGTLNLSANCLDRHLTE-RGEQTAIIWEGDDP 102
Cdd:cd05967 1 YEEVYARSIAEPEAFWAEQARLIDWFKPPEKILDNSNPPFT---RWFVGGRLNTCYNALDRHVEAgRGDQIALIYDSPVT 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 103 SQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKL 182
Cdd:cd05967 78 GTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAKELASRIDDAKPKL 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 183 VITADEGMRAGRAIPLKKNVDDALKNpAITSINNVVVFQRTG-KTGYWQEGRDLWWHDLIVGV-SADC-PAEemnAEDPL 259
Cdd:cd05967 158 IVTASCGIEPGKVVPYKPLLDKALEL-SGHKPHHVLVLNRPQvPADLTKPGRDLDWSELLAKAePVDCvPVA---ATDPL 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 260 FILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVP-NYPG 338
Cdd:cd05967 234 YILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIYGIKPGDVWWAASDVGWVVGHSYIVYGPLLHGATTVLYEGKPvGTPD 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 339 VNRLGQVIDKHQVNILYTAPTAIRALMAEgDKAIEGTKR---TSLRIMGSVGEPINPEAWEWYYNKIGngqCPIVDTWWQ 415
Cdd:cd05967 314 PGAFWRVIEKYQVNALFTAPTAIRAIRKE-DPDGKYIKKydlSSLRTLFLAGERLDPPTLEWAENTLG---VPVIDHWWQ 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 416 TETGGFMITPLPGATEL--KAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSW-PGQARTLYGDHDRFEQTYFSTF 492
Cdd:cd05967 390 TETGWPITANPVGLEPLpiKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIKLPLpPGCLLTLWKNDERFKKLYLSKF 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 493 KGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPT 572
Cdd:cd05967 470 PGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVVGVRDELKGQVPLGLVVLKEGVKIT 549
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1206237686 573 PE-LYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGDtsNLGDTSTLADPSVV 638
Cdd:cd05967 550 AEeLEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRKIADGE--DYTIPSTIEDPSVL 614
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
22-641 |
0e+00 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 615.03 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 22 AQYHDFYQQSVQNPDAFWGEQGKIIDWIKPYSIVKNTSFDPghvSIRWFEDGTLNLSANCLDRHLTERGEQTAIIWEGDD 101
Cdd:PRK10524 2 MSYSEFYQRSIDDPEAFWAEQARRIDWQTPFTQVLDYSNPP---FARWFVGGRTNLCHNAVDRHLAKRPEQLALIAVSTE 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 102 PSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAK 181
Cdd:PRK10524 79 TDEERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASHSLAARIDDAKPV 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 182 LVITADEGMRAGRAIPLKKNVDDALK----NPAitsinNVVVFQRTGKTGYWQEGRDLWWHDLIVGV-SADCPAEEMNAE 256
Cdd:PRK10524 159 LIVSADAGSRGGKVVPYKPLLDEAIAlaqhKPR-----HVLLVDRGLAPMARVAGRDVDYATLRAQHlGARVPVEWLESN 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 257 DPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNY 336
Cdd:PRK10524 234 EPSYILYTSGTTGKPKGVQRDTGGYAVALATSMDTIFGGKAGETFFCASDIGWVVGHSYIVYAPLLAGMATIMYEGLPTR 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 337 PGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNgqcPIVDTWWQT 416
Cdd:PRK10524 314 PDAGIWWRIVEKYKVNRMFSAPTAIRVLKKQDPALLRKHDLSSLRALFLAGEPLDEPTASWISEALGV---PVIDNYWQT 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 417 ETGGFMITPLPG--ATELKAGSATRPFFGVQPALVDNL-GNPQEGASEGNLVITDSW-PGQARTLYGDHDRFEQTYFSTF 492
Cdd:PRK10524 391 ETGWPILAIARGveDRPTRLGSPGVPMYGYNVKLLNEVtGEPCGPNEKGVLVIEGPLpPGCMQTVWGDDDRFVKTYWSLF 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 493 KGM-YFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHG--- 568
Cdd:PRK10524 471 GRQvYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPKDSdsl 550
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 569 --EEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAG-DTsnlGDTSTLADPSVVEKL 641
Cdd:PRK10524 551 adREARLALEKEIMALVDSQLGAVARPARVWFVSALPKTRSGKLLRRAIQAIAEGrDP---GDLTTIEDPAALQQI 623
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
22-635 |
0e+00 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 542.47 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 22 AQYHDFYQQSVQNPDAFWGEQGKIID--WIKPYSIVKNTSfdPGHVSIRWFEDGTLNLSANCLDRHLTERGEQTAIIWEG 99
Cdd:cd05968 7 PDLEAFLERSAEDNAWFWGEFVKDVGieWYEPPYQTLDLS--GGKPWAAWFVGGRMNIVEQLLDKWLADTRTRPALRWEG 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 100 DDpSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSN 179
Cdd:cd05968 85 ED-GTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATRLQDAE 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 180 AKLVITADEGMRAGRAIPLKKNVDDALKnpAITSINNVVVFQRTGKTGYWQEGRDLWWHDLIVGvsADCPAEEMNAEDPL 259
Cdd:cd05968 164 AKALITADGFTRRGREVNLKEEADKACA--QCPTVEKVVVVRHLGNDFTPAKGRDLSYDEEKET--AGDGAERTESEDPL 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 260 FILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGD-IYWCTaDVGWVTGhSYLLYGPLACGAMTLMFEGVPNYPG 338
Cdd:cd05968 240 MIIYTSGTTGKPKGTVHVHAGFPLKAAQDMYFQFDLKPGDlLTWFT-DLGWMMG-PWLIFGGLILGATMVLYDGAPDHPK 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 339 VNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNGQCPIVDTWWQTET 418
Cdd:cd05968 318 ADRLWRMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSSLRVLGSTGEPWNPEPWNWLFETVGKGRNPIINYSGGTEI 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 419 GGFMITPLPgATELKAGSATRPFFGVQPALVDNLGNPQEGaSEGNLVITDSWPGQARTLYGDHDRFEQTYFSTFKGMYFS 498
Cdd:cd05968 398 SGGILGNVL-IKPIKPSSFNGPVPGMKADVLDESGKPARP-EVGELVLLAPWPGMTRGFWRDEDRYLETYWSRFDNVWVH 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 499 GDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTE 578
Cdd:cd05968 476 GDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFVVLKPGVTPTEALAEE 555
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*..
gi 1206237686 579 VRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGDtsNLGDTSTLADP 635
Cdd:cd05968 556 LMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIRAAYLGK--ELGDLSSLENP 610
|
|
| propion_prpE |
TIGR02316 |
propionate--CoA ligase; This family contains one of three readily separable clades of proteins ... |
24-650 |
2.95e-173 |
|
propionate--CoA ligase; This family contains one of three readily separable clades of proteins in the group of acetate and propionate--CoA ligases. Characterized members of this family act on propionate. From propionyl-CoA, there is a cyclic degradation pathway: it is ligated by PrpC to the TCA cycle intermediate oxaloacetate, acted upon further by PrpD and an aconitase, then cleaved by PrpB to pyruvate and the TCA cycle intermediate succinate.
Pssm-ID: 131369 [Multi-domain] Cd Length: 628 Bit Score: 507.91 E-value: 2.95e-173
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 24 YHDFYQQSVQNPDAFWGEQGKIIDWIKPYSIVKNTSFDPghvSIRWFEDGTLNLSANCLDRHLTERGEQTAIIWEGDDPS 103
Cdd:TIGR02316 3 YEEFYQRSIEQPEAFWAEQARRIDWQTPPQRILDYSNPP---FARWFAGGRTNLCHNALDRHLDERGEQLALVTVSSETG 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 104 QSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLV 183
Cdd:TIGR02316 80 QERTLTYRQLHREVNVFASALRALGVGRGDRVLIYMPMIAEAVFAMLACARIGAIHSVVFGGFASHSLALRIDDATPKLI 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 184 ITADEGMRAGRAIPLKKNVDDALkNPAITSINNVVVFQRTGKTGYWQEGRDLWWHDLIVG-VSADCPAEEMNAEDPLFIL 262
Cdd:TIGR02316 160 VSADAGMRGGKVIPYKPLLDAAI-AEAQHPPPHVLLVDRGLAPMRLIPGRDVDYAALRTQhEDAQVPVEWLESNEPSYIL 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 263 YTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNYPGVNRL 342
Cdd:TIGR02316 239 YTSGTTGKPKGVQRDVGGYAVALALSMWAIFGIRAGQVMFSASDVGWVVGHSYIVYAPLLAGAATVLYEGLPTNPDPGVW 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 343 GQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNgqcPIVDTWWQTETGGFM 422
Cdd:TIGR02316 319 WSIVERYGVRTMFSAPTAIRVLKKQDAAWLRKHDLSSLHWLFLAGEPLDEPTAHWITDGLGK---PVIDNYWQTETGWPV 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 423 ITPLPGA--TELKAGSATRPFFGVQPALVDNL-GNPQEGASEGNLVITDSW-PGQARTLYGDHDRFEQTYFSTFKG-MYF 497
Cdd:TIGR02316 396 LAIMPGLdlKPVKLGSPGLPMYGYHLRVLDEAtGRPCGPNEKGVLTVVPPLpPGCLSTVWGDDARFLKTYWSHFKRpLYS 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 498 SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNH----GEEPTP 573
Cdd:TIGR02316 476 SFDWGIRDEDGYTFILGRTDDVINVAGHRLGTREIEESVSSHPSVAEVAVVGVHDELKGQVAVVFAILKEsdsaGDAHDP 555
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1206237686 574 -ELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGdtSNLGDTSTLADPSVVEKLleeKQSMQA 650
Cdd:TIGR02316 556 hAVETGMMDCVVRQLGAVARPARVYFVAALPKTRSGKLLRRSIQALAEG--RDPGDLTTIDDPGALEQV---RRAMER 628
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
69-628 |
4.70e-173 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 505.20 E-value: 4.70e-173
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 69 WFEDGTLNLSANCLDRHL-TERGEQTAIIWEGDDPSQSkhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAV 147
Cdd:PRK04319 36 WLETGKVNIAYEAIDRHAdGGRKDKVALRYLDASRKEK--YTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYF 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 148 AMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADEGMRagraiplKKNVDDalknpaITSINNVVVFQRTGKtg 227
Cdd:PRK04319 114 ALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLITTPALLE-------RKPADD------LPSLKHVLLVGEDVE-- 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 228 ywQEGRDLWWHDLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHttggylVYAAM-----TFKYVFDYHPGDIYW 302
Cdd:PRK04319 179 --EGPGTLDFNALMEQASDEFDIEWTDREDGAILHYTSGSTGKPKGVLH------VHNAMlqhyqTGKYVLDLHEDDVYW 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 303 CTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNypgVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRI 382
Cdd:PRK04319 251 CTADPGWVTGTSYGIFAPWLNGATNVIDGGRFS---PERWYRILEDYKVTVWYTAPTAIRMLMGAGDDLVKKYDLSSLRH 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 383 MGSVGEPINPEAWEWYYNKIGNgqcPIVDTWWQTETGGFMITPLPgATELKAGSATRPFFGVQPALVDNLGNPQEGASEG 462
Cdd:PRK04319 328 ILSVGEPLNPEVVRWGMKVFGL---PIHDNWWMTETGGIMIANYP-AMDIKPGSMGKPLPGIEAAIVDDQGNELPPNRMG 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 463 NLVITDSWPGQARTLYGDHDRFEQtYFStfKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKI 542
Cdd:PRK04319 404 NLAIKKGWPSMMRGIWNNPEKYES-YFA--GDWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAV 480
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 543 AEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK----I 618
Cdd:PRK04319 481 AEAGVIGKPDPVRGEIIKAFVALRPGYEPSEELKEEIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRRVLKAwelgL 560
|
570
....*....|
gi 1206237686 619 AAGDTSNLGD 628
Cdd:PRK04319 561 PEGDLSTMED 570
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
109-619 |
1.34e-148 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 437.70 E-value: 1.34e-148
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADE 188
Cdd:cd05969 2 TFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTEE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 189 gmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcPAEEMNAEDPLFILYTSGST 268
Cdd:cd05969 82 ------------------------------------------------------------LYERTDPEDPTLLHYTSGTT 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 269 GKPKGVVHTTGGYLVYAaMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNypgVNRLGQVIDK 348
Cdd:cd05969 102 GTPKGVLHVHDAMIFYY-FTGKYVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNGVTNVVYEGRFD---AESWYGIIER 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 349 HQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGngqCPIVDTWWQTETGGFMITPLPG 428
Cdd:cd05969 178 VKVTVWYTAPTAIRMLMKEGDELARKYDLSSLRFIHSVGEPLNPEAIRWGMEVFG---VPIHDTWWQTETGSIMIANYPC 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 429 aTELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSWPGQARTLYGDHDRFEQtYFSTfkGMYFSGDGARRDEDG 508
Cdd:cd05969 255 -MPIKPGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPGWPSMFRGIWNDEERYKN-SFID--GWYLTGDLAYRDEDG 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 509 YYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVRKEIG 588
Cdd:cd05969 331 YFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELKEEIINFVRQKLG 410
|
490 500 510
....*....|....*....|....*....|.
gi 1206237686 589 PIATPDILHWTDSLPKTRSGKIMRRILRKIA 619
Cdd:cd05969 411 AHVAPREIEFVDNLPKTRSGKIMRRVLKAKE 441
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
108-617 |
1.68e-121 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 367.82 E-value: 1.68e-121
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITad 187
Cdd:cd05972 1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVT-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 egmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemNAEDPLFILYTSGS 267
Cdd:cd05972 79 ------------------------------------------------------------------DAEDPALIYFTSGT 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 268 TGKPKGVVHTTGgYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNYPgvNRLGQVID 347
Cdd:cd05972 93 TGLPKGVLHTHS-YPLGHIPTAAYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVYEGPRFDA--ERILELLE 169
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 348 KHQVNILYTAPTAIRALMAEGdkaIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGngqCPIVDTWWQTETGgFMITPLP 427
Cdd:cd05972 170 RYGVTSFCGPPTAYRMLIKQD---LSSYKFSHLRLVVSAGEPLNPEVIEWWRAATG---LPIRDGYGQTETG-LTVGNFP 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 428 GaTELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSWPGQARTLYGDHDRFEqtyfSTFKG-MYFSGDGARRDE 506
Cdd:cd05972 243 D-MPVKPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKLPPPGLFLGYVGDPEKTE----ASIRGdYYLTGDRAYRDE 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 507 DGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVRKE 586
Cdd:cd05972 318 DGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEELAEELQGHVKKV 397
|
490 500 510
....*....|....*....|....*....|.
gi 1206237686 587 IGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:cd05972 398 LAPYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
82-523 |
3.46e-107 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 330.43 E-value: 3.46e-107
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAiiWEGDDPsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:pfam00501 1 LERQAARTPDKTA--LEVGEG---RRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVP 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVITADEgmragraiPLKKNVDDALKNPAITSINNVVVFQRtgktgyWQEGRDLWWHDLI 241
Cdd:pfam00501 76 LNPRLPAEELAYILEDSGAKVLITDDA--------LKLEELLEALGKLEVVKLVLVLDRDP------VLKEEPLPEEAKP 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 242 VGVsADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTgGYLVYAAMTFKYV----FDYHPGDIYWCTADVGWVTGHSYLL 317
Cdd:pfam00501 142 ADV-PPPPPPPPDPDDLAYIIYTSGTTGKPKGVMLTH-RNLVANVLSIKRVrprgFGLGPDDRVLSTLPLFHDFGLSLGL 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 318 YGPLACGAMTLMFEGVPNYPgVNRLGQVIDKHQVNILYTAPTAIRALMAEGdkAIEGTKRTSLRIMGSVGEPINPEAWEW 397
Cdd:pfam00501 220 LGPLLAGATVVLPPGFPALD-PAALLELIERYKVTVLYGVPTLLNMLLEAG--APKRALLSSLRLVLSGGAPLPPELARR 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 398 YYNKIGngqCPIVDTWWQTETGGFMITPLPGATEL-KAGSATRPFFGVQPALVD-NLGNPQEGASEGNLVItdSWPGQAR 475
Cdd:pfam00501 297 FRELFG---GALVNGYGLTETTGVVTTPLPLDEDLrSLGSVGRPLPGTEVKIVDdETGEPVPPGEPGELCV--RGPGVMK 371
|
410 420 430 440
....*....|....*....|....*....|....*....|....*...
gi 1206237686 476 TLYGDHDRFEQTYFStfKGMYFSGDGARRDEDGYYWITGRVDDVLNVS 523
Cdd:pfam00501 372 GYLNDPELTAEAFDE--DGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
82-623 |
1.25e-100 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 314.83 E-value: 1.25e-100
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:COG0318 5 LRRAAARHPDRPALVFGG------RRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVP 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVITAdegmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdli 241
Cdd:COG0318 79 LNPRLTAEELAYILEDSGARALVTA------------------------------------------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 242 vgvsadcpaeemnaedplFILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPL 321
Cdd:COG0318 104 ------------------LILYTSGTTGRPKGVMLTHRN-LLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPL 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 322 ACGA-MTLMFEGVPNypgvnRLGQVIDKHQVNILYTAPTAIRALMAEGDKAieGTKRTSLRIMGSVGEPINPEAWEWYYN 400
Cdd:COG0318 165 LAGAtLVLLPRFDPE-----RVLELIERERVTVLFGVPTMLARLLRHPEFA--RYDLSSLRLVVSGGAPLPPELLERFEE 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 401 KIGngqCPIVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVItdSWPGQARTLYGD 480
Cdd:COG0318 238 RFG---VRIVEGYGLTETSPVVTVNPEDPGERRPGSVGRPLPGVEVRIVDEDGRELPPGEVGEIVV--RGPNVMKGYWND 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 481 HDRFEQTyfstFK-GMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAI 559
Cdd:COG0318 313 PEATAEA----FRdGWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERV 388
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1206237686 560 YAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGDT 623
Cdd:COG0318 389 VAFVVLRPGAELDAE---ELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRERYAAGA 449
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
257-611 |
2.42e-94 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 294.19 E-value: 2.42e-94
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 257 DPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYvFDYHPGDIYWCTADVGWVtGHSYLLYGPLACGAMTLMFEGvPNY 336
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAAS-GGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLPK-FDP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 337 PGVNRLgqvIDKHQVNILYTAPTAIRALMAEGDKAieGTKRTSLRIMGSVGEPINPEAWEWYYNKIGngqCPIVDTWWQT 416
Cdd:cd04433 78 EAALEL---IEREKVTILLGVPTLLARLLKAPESA--GYDLSSLRALVSGGAPLPPELLERFEEAPG---IKLVNGYGLT 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 417 ETGGFMITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSWPGQartlyGDHDRFEQTYFSTFKGMY 496
Cdd:cd04433 150 ETGGTVATGPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMK-----GYWNNPEATAAVDEDGWY 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 497 FSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPEly 576
Cdd:cd04433 225 RTGDLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADLDAE-- 302
|
330 340 350
....*....|....*....|....*....|....*
gi 1206237686 577 tEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIM 611
Cdd:cd04433 303 -ELRAHVRERLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
109-617 |
4.04e-89 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 284.41 E-value: 4.04e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITade 188
Cdd:cd05973 2 TFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVT--- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 189 gmragraiplkkNVDdalknpaitsinnvvvfQRtgktgywqegrdlwwHDLivgvsadcpaeemnAEDPLFILYTSGST 268
Cdd:cd05973 79 ------------DAA-----------------NR---------------HKL--------------DSDPFVMMFTSGTT 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 269 GKPKGVVHTTGGYLVYAAMtFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNYPGVNRlgqVIDK 348
Cdd:cd05973 101 GLPKGVPVPLRALAAFGAY-LRDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPTILLEGGFSVESTWR---VIER 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 349 HQVNILYTAPTAIRALMAEGdKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGngqCPIVDTWWQTETGGFMITPLPG 428
Cdd:cd05973 177 LGVTNLAGSPTAYRLLMAAG-AEVPARPKGRLRRVSSAGEPLTPEVIRWFDAALG---VPIHDHYGQTELGMVLANHHAL 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 429 ATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVItdswpgqartlygDHDRFEQTYF--------STFKGMYF-SG 499
Cdd:cd05973 253 EHPVHAGSAGRAMPGWRVAVLDDDGDELGPGEPGRLAI-------------DIANSPLMWFrgyqlpdtPAIDGGYYlTG 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 500 DGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEV 579
Cdd:cd05973 320 DTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTPALADEL 399
|
490 500 510
....*....|....*....|....*....|....*...
gi 1206237686 580 RNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:cd05973 400 QLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLRR 437
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
24-639 |
3.45e-85 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 280.53 E-value: 3.45e-85
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 24 YHDFYQQSVQNPDAFWG---EQGKIIdWIKPYSIVKNTSFDPGhvsIRWFEDGTLNLSANCLdRHltERGEQTAIIWEGD 100
Cdd:PRK03584 36 YAALWRWSVEDLEAFWQsvwDFFGVI-GSTPYTVVLAGRRMPG---ARWFPGARLNYAENLL-RH--RRDDRPAIIFRGE 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 101 DpSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNA 180
Cdd:PRK03584 109 D-GPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFGVQGVLDRFGQIEP 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 181 KLVITADeGMR-AGRAIPLKKNVDDALKnpAITSINNVVVFQRTGKTG-YWQEGRDLWWHDLIVGVSADCPA-EEMNAED 257
Cdd:PRK03584 188 KVLIAVD-GYRyGGKAFDRRAKVAELRA--ALPSLEHVVVVPYLGPAAaAAALPGALLWEDFLAPAEAAELEfEPVPFDH 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 258 PLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGD-IYWCTAdVGW------VTGhsyllygpLACGAMTLMF 330
Cdd:PRK03584 265 PLWILYSSGTTGLPKCIVHGHGGILLEHLKELGLHCDLGPGDrFFWYTT-CGWmmwnwlVSG--------LLVGATLVLY 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 331 EGVPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNgqcpiv 410
Cdd:PRK03584 336 DGSPFYPDPNVLWDLAAEEGVTVFGTSAKYLDACEKAGLVPGETHDLSALRTIGSTGSPLPPEGFDWVYEHVKA------ 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 411 DTWWQTETGG------FMI-TPL----PGatELKAgsatrPFFGVQPALVDNLGNPQEGAsEGNLVITDSWPGQARTLYG 479
Cdd:PRK03584 410 DVWLASISGGtdicscFVGgNPLlpvyRG--EIQC-----RGLGMAVEAWDEDGRPVVGE-VGELVCTKPFPSMPLGFWN 481
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 480 DHD--RFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQ 557
Cdd:PRK03584 482 DPDgsRYRDAYFDTFPGVWRHGDWIEITEHGGVVIYGRSDATLNRGGVRIGTAEIYRQVEALPEVLDSLVIGQEWPDGDV 561
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 558 AIYAYITLNHGEEPTPELYTEVRNWVRKEIGP-------IATPDIlhwtdslPKTRSGKIM----RRILRKIAAGDTSNL 626
Cdd:PRK03584 562 RMPLFVVLAEGVTLDDALRARIRTTIRTNLSPrhvpdkiIAVPDI-------PRTLSGKKVelpvKKLLHGRPVKKAVNR 634
|
650
....*....|...
gi 1206237686 627 GdtsTLADPSVVE 639
Cdd:PRK03584 635 D---ALANPEALD 644
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
22-635 |
7.26e-85 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 278.77 E-value: 7.26e-85
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 22 AQYHDFYQQSVQNPDAFWGEqgkIIDWikpYSIVKNTSFDPGHVS------IRWFEDGTLNLsANCLDRHlTERGEQTAI 95
Cdd:cd05943 17 ADYAALHRWSVDDPGAFWAA---VWDF---SGVRGSKPYDVVVVSgrimpgARWFPGARLNY-AENLLRH-ADADDPAAI 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 96 IWEGDDPSQskHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRI 175
Cdd:cd05943 89 YAAEDGERT--EVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGVPGVLDRF 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 176 IDSNAKLVITADEGMRAGRAIPLKKNVDDALKnpAITSINNVVVFQRT---GKTGYWQEGRDLWWHDLIV-GVSADCPAE 251
Cdd:cd05943 167 GQIEPKVLFAVDAYTYNGKRHDVREKVAELVK--GLPSLLAVVVVPYTvaaGQPDLSKIAKALTLEDFLAtGAAGELEFE 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 252 EMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSylLYGPLACGAMTLMFE 331
Cdd:cd05943 245 PLPFDHPLYILYSSGTTGLPKCIVHGAGGTLLQHLKEHILHCDLRPGDRLFYYTTCGWMMWNW--LVSGLAVGATIVLYD 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 332 GVPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNgqcpivD 411
Cdd:cd05943 323 GSPFYPDTNALWDLADEEGITVFGTSAKYLDALEKAGLKPAETHDLSSLRTILSTGSPLKPESFDYVYDHIKP------D 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 412 TWWQTETGG------FMIT-PL----PGatELKAgsatrPFFGVQPALVDNLGNPQEGASeGNLVITDSWPGQARTLYGD 480
Cdd:cd05943 397 VLLASISGGtdiiscFVGGnPLlpvyRG--EIQC-----RGLGMAVEAFDEEGKPVWGEK-GELVCTKPFPSMPVGFWND 468
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 481 HD--RFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQA 558
Cdd:cd05943 469 PDgsRYRAAYFAKYPGVWAHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDER 548
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1206237686 559 IYAYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGdtSNLGDTSTLADP 635
Cdd:cd05943 549 VILFVKLREGVELDDELRKRIRSTIRSALSPRHVPAKIIAVPDIPRTLSGKKVEVAVKKIIAG--RPVKNAGALANP 623
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
105-616 |
1.25e-75 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 248.89 E-value: 1.25e-75
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 105 SKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVI 184
Cdd:cd05971 4 PEKVTFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 185 TaDEgmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemnAEDPLFILYT 264
Cdd:cd05971 84 T-DG------------------------------------------------------------------SDDPALIIYT 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 265 SGSTGKPKGVVHTTG---GYLVYAAMTFKyvFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNYPGvnR 341
Cdd:cd05971 97 SGTTGPPKGALHAHRvllGHLPGVQFPFN--LFPRDGDLYWTPADWAWIGGLLDVLLPSLYFGVPVLAHRMTKFDPK--A 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 342 LGQVIDKHQVNILYTAPTAIRALMAEGDKaiEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGngqCPIVDTWWQTEtGGF 421
Cdd:cd05971 173 ALDLMSRYGVTTAFLPPTALKMMRQQGEQ--LKHAQVKLRAIATGGESLGEELLGWAREQFG---VEVNEFYGQTE-CNL 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 422 MITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSWPGQARTlYGDHDRFEQtyfSTFKGMYF-SGD 500
Cdd:cd05971 247 VIGNCSALFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEIAVELPDPVAFLG-YWNNPSATE---KKMAGDWLlTGD 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 501 GARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVR 580
Cdd:cd05971 323 LGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETPSDALAREIQ 402
|
490 500 510
....*....|....*....|....*....|....*.
gi 1206237686 581 NWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd05971 403 ELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELR 438
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
74-616 |
8.47e-74 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 247.02 E-value: 8.47e-74
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 74 TLNLSANCLDRHLTERGEQTAIIWeGDDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACA 153
Cdd:cd05970 15 NFNFAYDVVDAMAKEYPDKLALVW-CDDAGEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALH 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 154 RIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADEgmragraiplkKNVDDALKNpAITSINNVVVFQRTGktgywQEGR 233
Cdd:cd05970 94 KLGAIAIPATHQLTAKDIVYRIESADIKMIVAIAE-----------DNIPEEIEK-AAPECPSKPKLVWVG-----DPVP 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 234 DLW--WHDLIVGVSADCPAEEMNA----EDPLFILYTSGSTGKPKGVVHTTG---GYLVyaamTFKYVFDYHPGDIYWCT 304
Cdd:cd05970 157 EGWidFRKLIKNASPDFERPTANSypcgEDILLVYFSSGTTGMPKMVEHDFTyplGHIV----TAKYWQNVREGGLHLTV 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 305 ADVGWVTGHSYLLYGPLACGAMTLMFEGVPNYPgvNRLGQVIDKHQVNILYTAPTAIRALMAEGdkaIEGTKRTSLRIMG 384
Cdd:cd05970 233 ADTGWGKAVWGKIYGQWIAGAAVFVYDYDKFDP--KALLEKLSKYGVTTFCAPPTIYRFLIRED---LSRYDLSSLRYCT 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 385 SVGEPINPEAWEWYYNKIGngqCPIVDTWWQTETGgFMITPLPGaTELKAGSATRPFFGVQPALVDNLGNPQEGASEGNL 464
Cdd:cd05970 308 TAGEALNPEVFNTFKEKTG---IKLMEGFGQTETT-LTIATFPW-MEPKPGSMGKPAPGYEIDLIDREGRSCEAGEEGEI 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 465 VI--TDSWP-GQARTLYGDHDRFEQTYFStfkGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPK 541
Cdd:cd05970 383 VIrtSKGKPvGLFGGYYKDAEKTAEVWHD---GYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALIQHPA 459
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1206237686 542 IAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd05970 460 VLECAVTGVPDPIRGQVVKATIVLAKGYEPSEELKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIR 534
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
18-617 |
2.92e-72 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 245.81 E-value: 2.92e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 18 LINPAQYHDFYQQSVQNPDAFWGEQG-KIIDWIKPYSIVkntsFDPGHVSIRWFEDGTLNLSANCLDRHLT--ERGEQTA 94
Cdd:PTZ00237 4 LSDPFDYENDSNYANSNPESFWDEVAkKYVHWDKMYDKV----YSGDEIYPDWFKGGELNTCYNVLDIHVKnpLKRDQDA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 95 IIWEGDDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGR 174
Cdd:PTZ00237 80 LIYECPYLKKTIKLTYYQLYEKVCEFSRVLLNLNISKNDNVLIYMANTLEPLIAMLSCARIGATHCVLFDGYSVKSLIDR 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 175 IIDSNAKLVITADEGMRAGRAIPLKKNVDDALKNPAITSiNNVVVFQRTGKTGYWQEGR---------DLWWHDLIVGVS 245
Cdd:PTZ00237 160 IETITPKLIITTNYGILNDEIITFTPNLKEAIELSTFKP-SNVITLFRNDITSESDLKKietiptipnTLSWYDEIKKIK 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 246 AD--------CPAEemnAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYlL 317
Cdd:PTZ00237 239 ENnqspfyeyVPVE---SSHPLYILYTSGTTGNSKAVVRSNGPHLVGLKYYWRSIIEKDIPTVVFSHSSIGWVSFHGF-L 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 318 YGPLACGAMTLMFEG--VPNYPGVNRLGQVIDKHQVNILYTAPTAIRALM---AEGDKAIEGTKRTSLRIMGSVGEPINP 392
Cdd:PTZ00237 315 YGSLSLGNTFVMFEGgiIKNKHIEDDLWNTIEKHKVTHTLTLPKTIRYLIktdPEATIIRSKYDLSNLKEIWCGGEVIEE 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 393 EAWEWYYNKIGngqCPIVDTWWQTETGgfmITPLPGATELKAGSAT--RPFFGVQPALVDN----LGNPQEGASEGNLVI 466
Cdd:PTZ00237 395 SIPEYIENKLK---IKSSRGYGQTEIG---ITYLYCYGHINIPYNAtgVPSIFIKPSILSEdgkeLNVNEIGEVAFKLPM 468
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 467 TdswPGQARTLYGDHDRFEQTyFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAA 546
Cdd:PTZ00237 469 P---PSFATTFYKNDEKFKQL-FSKFPGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECC 544
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1206237686 547 VVGVPHNIKGQAIYAYITLNHGEEPTP----ELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:PTZ00237 545 SIGIYDPDCYNVPIGLLVLKQDQSNQSidlnKLKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPRQIISK 619
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
79-616 |
1.62e-71 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 239.96 E-value: 1.62e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 79 ANCLDRHLTE-RGEQTAIIwegDDPSqskHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGA 157
Cdd:cd05959 6 ATLVDLNLNEgRGDKTAFI---DDAG---SLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 158 VHSVIFGGFSPDAVAGRIIDSNAKLVITADEgmragraipLKKNVDDALkNPAITSINNVVVFQrtgktGYWQEGRDLWW 237
Cdd:cd05959 80 VPVPVNTLLTPDDYAYYLEDSRARVVVVSGE---------LAPVLAAAL-TKSEHTLVVLIVSG-----GAGPEAGALLL 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 238 HDLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTF-KYVFDYHPGDIYWCTADVGWVTGHSYL 316
Cdd:cd05959 145 AELVAAEAEQLKPAATHADDPAFWLYSSGSTGRPKGVVHLHAD-IYWTAELYaRNVLGIREDDVCFSAAKLFFAYGLGNS 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 317 LYGPLACGAMTLMFegvPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAegDKAIEGTKRTSLRIMGSVGEPINPEAWE 396
Cdd:cd05959 224 LTFPLSVGATTVLM---PERPTPAAVFKRIRRYRPTVFFGVPTLYAAMLA--APNLPSRDLSSLRLCVSAGEALPAEVGE 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 397 WYYNKIGngqCPIVDTWWQTETGGFMITPLPGATELkaGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDswpGQART 476
Cdd:cd05959 299 RWKARFG---LDILDGIGSTEMLHIFLSNRPGRVRY--GTTGKPVPGYEVELRDEDGGDVADGEPGELYVRG---PSSAT 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 477 LYgdHDRFEQTYfSTFKGMYF-SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIK 555
Cdd:cd05959 371 MY--WNNRDKTR-DTFQGEWTrTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDG 447
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 556 GQAIYAYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd05959 448 LTKPKAFVVLRPGYEDSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
72-622 |
4.68e-70 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 236.62 E-value: 4.68e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 72 DGTLNLsANCLDRHLTERGEQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLA 151
Cdd:PRK06187 3 DYPLTI-GRILRHGARKHPDKEAVYFDG------RRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFA 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 152 CARIGAV-HSV-IFggFSPDAVAGRIIDSNAKLVITADEGmragraIPLKKNVddalkNPAITSINNVVVF---QRTGKT 226
Cdd:PRK06187 76 VPKIGAVlHPInIR--LKPEEIAYILNDAEDRVVLVDSEF------VPLLAAI-----LPQLPTVRTVIVEgdgPAAPLA 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 227 GYWQEgrdlwWHDLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTtggY--LVYAAMTFKYVFDYHPGDIYwct 304
Cdd:PRK06187 143 PEVGE-----YEELLAAASDTFDFPDIDENDAAAMLYTSGTTGHPKGVVLS---HrnLFLHSLAVCAWLKLSRDDVY--- 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 305 advgwvtghsyLLYGPLA-CGAMTL----MFEGVPN-YPG---VNRLGQVIDKHQVNILYTAPTAIRALMAEgdKAIEGT 375
Cdd:PRK06187 212 -----------LVIVPMFhVHAWGLpylaLMAGAKQvIPRrfdPENLLDLIETERVTFFFAVPTIWQMLLKA--PRAYFV 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 376 KRTSLRIMGSVGEPINPEAWEWYYNKIGngqCPIVDTWWQTETGGFM-ITPLP---GATELKAGSATRPFFGVQPALVDN 451
Cdd:PRK06187 279 DFSSLRLVIYGGAALPPALLREFKEKFG---IDLVQGYGMTETSPVVsVLPPEdqlPGQWTKRRSAGRPLPGVEARIVDD 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 452 LGN--PQEGASEGNLVITDSWPGQArtLYGDHDRFEQTYFstfKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGT 529
Cdd:PRK06187 356 DGDelPPDGGEVGEIIVRGPWLMQG--YWNRPEATAETID---GGWLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYP 430
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 530 AEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGK 609
Cdd:PRK06187 431 RELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATLDAK---ELRAFLRGRLAKFKLPKRIAFVDELPRTSVGK 507
|
570
....*....|....
gi 1206237686 610 IMRRILR-KIAAGD 622
Cdd:PRK06187 508 ILKRVLReQYAEGK 521
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
92-616 |
3.62e-68 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 228.89 E-value: 3.62e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 92 QTAIIwegdDPSQSkhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAV 171
Cdd:cd05919 1 KTAFY----AADRS--VTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDY 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 172 AGRIIDSNAKLVITadegmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpae 251
Cdd:cd05919 75 AYIARDCEARLVVT------------------------------------------------------------------ 88
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 252 emNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADV--GWVTGHSylLYGPLACGAMTLM 329
Cdd:cd05919 89 --SADDIAYLLYSSGTTGPPKGVMHAHRDPLLFADAMAREALGLTPGDRVFSSAKMffGYGLGNS--LWFPLAVGASAVL 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 330 FegvPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKrtSLRIMGSVGEPINPEAWEWYYNKIGngqCPI 409
Cdd:cd05919 165 N---PGWPTAERVLATLARFRPTVLYGVPTFYANLLDSCAGSPDALR--SLRLCVSAGEALPRGLGERWMEHFG---GPI 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 410 VDTWWQTETGGFMITPLPGATELkaGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDswPGQARTLYgdhDRFEQTYF 489
Cdd:cd05919 237 LDGIGATEVGHIFLSNRPGAWRL--GSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLVRG--PSAAVGYW---NNPEKSRA 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 490 STFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGE 569
Cdd:cd05919 310 TFNGGWYRTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPA 389
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 1206237686 570 EPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd05919 390 APQESLARDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
88-617 |
8.87e-66 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 225.42 E-value: 8.87e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 88 ERGEQTAIIWEGDDPSQSKHvTYKQLHHNVCQFANVLKKL-GVKKGDVVAIYMPMVPEAAVAMLACARIGAVhsVIFGGF 166
Cdd:cd05928 23 KRPPNPALWWVNGKGDEVKW-SFRELGSLSRKAANVLSGAcGLQRGDRVAVILPRVPEWWLVNVACIRTGLV--FIPGTI 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 167 SPDA--VAGRIIDSNAKLVITADEgmragraipLKKNVDD-ALKNPAITSinnvvvfqrtgKTGYWQEGRDLW--WHDLI 241
Cdd:cd05928 100 QLTAkdILYRLQASKAKCIVTSDE---------LAPEVDSvASECPSLKT-----------KLLVSEKSRDGWlnFKELL 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 242 VGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPL 321
Cdd:cd05928 160 NEASTEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGLKVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEPW 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 322 ACGAMTLMFEgVPNYpGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIegtKRTSLRIMGSVGEPINPEAWEWYYNK 401
Cdd:cd05928 240 IQGACVFVHH-LPRF-DPLVILKTLSSYPITTFCGAPTVYRMLVQQDLSSY---KFPSLQHCVTGGEPLNPEVLEKWKAQ 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 402 IGngqCPIVDTWWQTETGgfMITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSwPGQARTLYGDH 481
Cdd:cd05928 315 TG---LDIYEGYGQTETG--LICANFKGMKIKPGSMGKASPPYDVQIIDDNGNVLPPGTEGDIGIRVK-PIRPFGLFSGY 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 482 -DRFEQTYfSTFKG-MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAI 559
Cdd:cd05928 389 vDNPEKTA-ATIRGdFYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVV 467
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1206237686 560 YAYITLN-----HGEEptpELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:cd05928 468 KAFVVLApqflsHDPE---QLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRD 527
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
82-612 |
3.81e-65 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 220.94 E-value: 3.81e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:cd17631 1 LRRRARRHPDRTALVFGG------RSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVP 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVItadegmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdli 241
Cdd:cd17631 75 LNFRLTPPEVAYILADSGAKVLF--------------------------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 242 vgvsadcpaeemnaEDPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADvgwvtghsylLY--G 319
Cdd:cd17631 98 --------------DDLALLMYTSGTTGRPKGAMLTHRN-LLWNAVNALAALDLGPDDVLLVVAP----------LFhiG 152
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 320 PLACGAMTLMFEGVPNY----PGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDkaIEGTKRTSLRIMGSVGEPINP--- 392
Cdd:cd17631 153 GLGVFTLPTLLRGGTVVilrkFDPETVLDLIERHRVTSFFLVPTMIQALLQHPR--FATTDLSSLRAVIYGGAPMPErll 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 393 EAWEWYYnkigngqCPIVDTWWQTETGGfMITPL-PGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVItdSWP 471
Cdd:cd17631 231 RALQARG-------VKFVQGYGMTETSP-GVTFLsPEDHRRKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVV--RGP 300
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 472 GQARtlyGDHDRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVP 551
Cdd:cd17631 301 HVMA---GYWNRPEATAAAFRDGWFHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVP 377
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 552 HNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMR 612
Cdd:cd17631 378 DEKWGEAVVAVVVPRPGAELDED---ELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
82-616 |
4.51e-60 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 208.19 E-value: 4.51e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAV--- 158
Cdd:cd05936 5 LEEAARRFPDKTALIFMG------RKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVvvp 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 159 HSVIFGgfsPDAVAGRIIDSNAKLVITAdegmragraIPLKknvdDALKNPAitsinnvvvfqrtgKTGYWQEgrdlwwh 238
Cdd:cd05936 79 LNPLYT---PRELEHILNDSGAKALIVA---------VSFT----DLLAAGA--------------PLGERVA------- 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 239 dlivgvsadcpaeeMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGD-----------IYWCTAdv 307
Cdd:cd05936 122 --------------LTPEDVAVLQYTSGTTGVPKGAMLTHRNLVANALQIKAWLEDLLEGDdvvlaalplfhVFGLTV-- 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 308 gwvtghSYLLygPLACGAMTLMfegVPNyPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKrtSLRIMGSVG 387
Cdd:cd05936 186 ------ALLL--PLALGATIVL---IPR-FRPIGVLKEIRKHRVTIFPGVPTMYIALLNAPEFKKRDFS--SLRLCISGG 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 388 EPINPEAWEWYYNKIGngqCPIVDTWWQTETG-GFMITPLPGATelKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVI 466
Cdd:cd05936 252 APLPVEVAERFEELTG---VPIVEGYGLTETSpVVAVNPLDGPR--KPGSIGIPLPGTEVKIVDDDGEELPPGEVGELWV 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 467 TdswpGQARTLyGDHDRFEQTYfSTFK-GMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEA 545
Cdd:cd05936 327 R----GPQVMK-GYWNRPEETA-EAFVdGWLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEA 400
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 546 AVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd05936 401 AVVGVPDPYSGEAVKAFVVLKEGASLTEE---EIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
22-617 |
2.88e-59 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 211.86 E-value: 2.88e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 22 AQYHDFYQQSVQNPDAFWG---EQGKIIDWIKPYSIVKNTSF-DPGHvsiRWFEDGTLNLSANCLDRHLTERGEQTAIIW 97
Cdd:PLN03052 120 SSFSEFQRFSVENPEVYWSivlDELSLVFSVPPRCILDTSDEsNPGG---QWLPGAVLNVAECCLTPKPSKTDDSIAIIW 196
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 98 --EGDDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRI 175
Cdd:PLN03052 197 rdEGSDDLPVNRMTLSELRSQVSRVANALDALGFEKGDAIAIDMPMNVHAVIIYLAIILAGCVVVSIADSFAPSEIATRL 276
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 176 IDSNAKLVITADEGMRAGRAIPLKKNVDDALKNPAI---TSINNVVVFQRTGktgywqegrDLWWHDLIVGVSA-----D 247
Cdd:PLN03052 277 KISKAKAIFTQDVIVRGGKSIPLYSRVVEAKAPKAIvlpADGKSVRVKLREG---------DMSWDDFLARANGlrrpdE 347
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 248 CPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVfDYHPGDIY-WCTaDVGWVTGHsYLLYGPLACGAM 326
Cdd:PLN03052 348 YKAVEQPVEAFTNILFSSGTTGEPKAIPWTQLTPLRAAADAWAHL-DIRKGDIVcWPT-NLGWMMGP-WLVYASLLNGAT 424
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 327 TLMFEGVPNYPGvnrLGQVIDKHQVNILYTAPTAIRALMAEGdkAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNGq 406
Cdd:PLN03052 425 LALYNGSPLGRG---FAKFVQDAKVTMLGTVPSIVKTWKNTN--CMAGLDWSSIRCFGSTGEASSVDDYLWLMSRAGYK- 498
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 407 cPIVDTWWQTETGG-FMITPLPGATELKAGSAtrPFFGVQPALVDNLGN--PQEGASEGNLVITDSWPGQARTLY-GDHD 482
Cdd:PLN03052 499 -PIIEYCGGTELGGgFVTGSLLQPQAFAAFST--PAMGCKLFILDDSGNpyPDDAPCTGELALFPLMFGASSTLLnADHY 575
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 483 rfeQTYfstFKGM-YFSGDGARRDED-------GYYWITGRVDDVLNVSGHRLGTAEIESAL-VSHPKIAEAAVVGVPHN 553
Cdd:PLN03052 576 ---KVY---FKGMpVFNGKILRRHGDifertsgGYYRAHGRADDTMNLGGIKVSSVEIERVCnAADESVLETAAIGVPPP 649
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1206237686 554 IKGQ---AIYAYITLNHGEEPTPELYTEVRN-WVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:PLN03052 650 GGGPeqlVIAAVLKDPPGSNPDLNELKKIFNsAIQKKLNPLFKVSAVVIVPSFPRTASNKVMRRVLRQ 717
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
102-617 |
8.57e-59 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 205.62 E-value: 8.57e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 102 PSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAK 181
Cdd:cd05926 9 PGSTPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSK 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 182 LVITADEGmrAGRAIPLKKNVDDALKNPAITSINNVVVFQrtgktgywqeGRDLwwHDLIVGVSADCPAEEMNAEDPLFI 261
Cdd:cd05926 89 LVLTPKGE--LGPASRAASKLGLAILELALDVGVLIRAPS----------AESL--SNLLADKKNAKSEGVPLPDDLALI 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 262 LYTSGSTGKPKGV--VHTTggyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGA---MTLMFEGvpny 336
Cdd:cd05926 155 LHTSGTTGRPKGVplTHRN---LAASATNITNTYKLTPDDRTLVVMPLFHVHGLVASLLSTLAAGGsvvLPPRFSA---- 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 337 pgvNRLGQVIDKHQVNiLYTA-PTAIRALMAEgDKAIEGTKRTSLRIMGSVGEPINP---EAWEWYYNkigngqCPIVDT 412
Cdd:cd05926 228 ---STFWPDVRDYNAT-WYTAvPTIHQILLNR-PEPNPESPPPKLRFIRSCSASLPPavlEALEATFG------APVLEA 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 413 WWQTETGGFMIT-PLPGATElKAGSATRPFfGVQPALVDNLGNPQEGASEGNLVITDSwpgqaRTLYGDHDRFEQTYFST 491
Cdd:cd05926 297 YGMTEAAHQMTSnPLPPGPR-KPGSVGKPV-GVEVRILDEDGEILPPGVVGEICLRGP-----NVTRGYLNNPEANAEAA 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 492 FKGMYF-SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEE 570
Cdd:cd05926 370 FKDGWFrTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLREGAS 449
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 1206237686 571 PTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:cd05926 450 VTEE---ELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
107-616 |
1.74e-58 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 202.52 E-value: 1.74e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 107 HVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITa 186
Cdd:cd05934 3 RWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVV- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 187 degmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemnaeDPLFILYTSG 266
Cdd:cd05934 82 ----------------------------------------------------------------------DPASILYTSG 91
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 267 STGKPKGVVhTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGA-MTLmfegVPNYpGVNRLGQV 345
Cdd:cd05934 92 TTGPPKGVV-ITHANLTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGAtLVL----LPRF-SASRFWSD 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 346 IDKHQVNILYTAPTAIRALMAEGDKAIEgtKRTSLRIMGsvGEPINPEAWEWYYNKIGngqCPIVDTWWQTETGGFMITP 425
Cdd:cd05934 166 VRRYGATVTNYLGAMLSYLLAQPPSPDD--RAHRLRAAY--GAPNPPELHEEFEERFG---VRLLEGYGMTETIVGVIGP 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 426 LPGATelKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITdSWPGQARTLyGDHDRFEQTYFSTFKGMYFSGDGARRD 505
Cdd:cd05934 239 RDEPR--RPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIR-GLRGWGFFK-GYYNMPEATAEAMRNGWFHTGDLGYRD 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 506 EDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRK 585
Cdd:cd05934 315 ADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPE---ELFAFCEG 391
|
490 500 510
....*....|....*....|....*....|.
gi 1206237686 586 EIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd05934 392 QLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
108-619 |
5.71e-57 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 198.95 E-value: 5.71e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVhsVIfggfspdavagriidsNAKLVITAD 187
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAV--VI----------------PATTLLTPD 62
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 EgmragraipLKKNVDdalknpaitsinnvvvfqrtgktgywqegrdlwwhdliVGVSADCPAEEMN-AEDPLFILYTSG 266
Cdd:cd05974 63 D---------LRDRVD--------------------------------------RGGAVYAAVDENThADDPMLLYFTSG 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 267 STGKPKGVVHTTGGYLVYAAMTFkYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFegvpNYPGVN--RLGQ 344
Cdd:cd05974 96 TTSKPKLVEHTHRSYPVGHLSTM-YWIGLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATVFLF----NYARFDakRVLA 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 345 VIDKHQVNILYTAPTAIRALMAEGDKAIegtkRTSLRIMGSVGEPINPEAWEWYYNKIGngqCPIVDTWWQTETGGfMIT 424
Cdd:cd05974 171 ALVRYGVTTLCAPPTVWRMLIQQDLASF----DVKLREVVGAGEPLNPEVIEQVRRAWG---LTIRDGYGQTETTA-LVG 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 425 PLPGATeLKAGSATRPFFGVQPALVDNLGNPqegASEGN--LVITDSWP-GQARTLYGDHDRfeqTYFSTFKGMYFSGDG 501
Cdd:cd05974 243 NSPGQP-VKAGSMGRPLPGYRVALLDPDGAP---ATEGEvaLDLGDTRPvGLMKGYAGDPDK---TAHAMRGGYYRTGDI 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 502 ARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRN 581
Cdd:cd05974 316 AMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPSPETALEIFR 395
|
490 500 510
....*....|....*....|....*....|....*...
gi 1206237686 582 WVRKEIGPIATPDILHWTDsLPKTRSGKIMRRILRKIA 619
Cdd:cd05974 396 FSRERLAPYKRIRRLEFAE-LPKTISGKIRRVELRRRE 432
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
101-611 |
2.77e-56 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 198.59 E-value: 2.77e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 101 DPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNA 180
Cdd:cd05911 4 DADTGKELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKP 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 181 KLVITADEGmragraiplKKNVDDALKNpaITSINNVVVFqrTGKTGYWQEGRDLWWHDLIVGVSADCPAEEMNAEDPLF 260
Cdd:cd05911 84 KVIFTDPDG---------LEKVKEAAKE--LGPKDKIIVL--DDKPDGVLSIEDLLSPTLGEEDEDLPPPLKDGKDDTAA 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 261 ILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFD-YHPGDIYWCTADVGWVTGHSYLLYGPLaCGAMTLMFegvpNYPGV 339
Cdd:cd05911 151 ILYSSGTTGLPKGVCLSHRNLIANLSQVQTFLYGnDGSNDVILGFLPLYHIYGLFTTLASLL-NGATVIIM----PKFDS 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 340 NRLGQVIDKHQVNILYTAPtAIRALMAEgDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNgqCPIVDTWWQTETG 419
Cdd:cd05911 226 ELFLDLIEKYKITFLYLVP-PIAAALAK-SPLLDKYDLSSLRVILSGGAPLSKELQELLAKRFPN--ATIKQGYGMTETG 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 420 GfMITPLPGaTELKAGSATRPFFGVQPALVDNLGNPQEGASE-GNLVItdsWPGQArtLYGDHDRFEQTYFSTFKGMYF- 497
Cdd:cd05911 302 G-ILTVNPD-GDDKPGSVGRLLPNVEAKIVDDDGKDSLGPNEpGEICV---RGPQV--MKGYYNNPEATKETFDEDGWLh 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 498 SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElyt 577
Cdd:cd05911 375 TGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPGEKLTEK--- 451
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 1206237686 578 EVRNWVRKEIgpiatPDILHW------TDSLPKTRSGKIM 611
Cdd:cd05911 452 EVKDYVAKKV-----ASYKQLrggvvfVDEIPKSASGKIL 486
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
91-624 |
1.26e-55 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 197.46 E-value: 1.26e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDA 170
Cdd:PRK08316 26 DKTALVFGD------RSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 171 VAGRIIDSNAKLVITADEgmragraipLKKNVDDAL-KNPAITSINNVVVFQRTGktgywqEGRDLWWHDLIVGVSADCP 249
Cdd:PRK08316 100 LAYILDHSGARAFLVDPA---------LAPTAEAALaLLPVDTLILSLVLGGREA------PGGWLDFADWAEAGSVAEP 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 250 AEEMNAEDPLFILYTSGSTGKPKGVVHTTGgylvyaAMTFKYV-----FDYHPGDI-------YWCTADvgwvtgHSYLl 317
Cdd:PRK08316 165 DVELADDDLAQILYTSGTESLPKGAMLTHR------ALIAEYVscivaGDMSADDIplhalplYHCAQL------DVFL- 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 318 yGP-LACGAMTLMFEGvpnyPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDkaIEGTKRTSLR-------IMGsvGEP 389
Cdd:PRK08316 232 -GPyLYVGATNVILDA----PDPELILRTIEAERITSFFAPPTVWISLLRHPD--FDTRDLSSLRkgyygasIMP--VEV 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 390 IN------PEAWewYYNKIGngqcpivdtwwQTEtggfmITPL-----PGATELKAGSATRPFFGVQPALVDNLGNPqeg 458
Cdd:PRK08316 303 LKelrerlPGLR--FYNCYG-----------QTE-----IAPLatvlgPEEHLRRPGSAGRPVLNVETRVVDDDGND--- 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 459 asegnlvITDSWPG-------QARTLYGDHDrfEQTYfSTFKGMYF-SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTA 530
Cdd:PRK08316 362 -------VAPGEVGeivhrspQLMLGYWDDP--EKTA-EAFRGGWFhSGDLGVMDEEGYITVVDRKKDMIKTGGENVASR 431
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 531 EIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKI 610
Cdd:PRK08316 432 EVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATVTED---ELIAHCRARLAGFKVPKRVIFVDELPRNPSGKI 508
|
570
....*....|....
gi 1206237686 611 MRRILRKIAAGDTS 624
Cdd:PRK08316 509 LKRELRERYAGAFT 522
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
73-617 |
4.08e-55 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 195.83 E-value: 4.08e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 73 GTLNLSANCLDRHLTE-RGEQTAIIwegdDPSQSkhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLA 151
Cdd:TIGR02262 1 EKYNAAEDLLDRNVVEgRGGKTAFI----DDISS--LSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLG 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 152 CARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADEgmragrAIPLKKNVddALKNPAITSInnVVVfqrtgktGYWQE 231
Cdd:TIGR02262 75 AIRAGIVPVALNTLLTADDYAYMLEDSRARVVFVSGA------LLPVIKAA--LGKSPHLEHR--VVV-------GRPEA 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 232 GRDLWWHDLIVGVSADCPAEeMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVT 311
Cdd:TIGR02262 138 GEVQLAELLATESEQFKPAA-TQADDPAFWLYSSGSTGMPKGVVHTHSNPYWTAELYARNTLGIREDDVCFSAAKLFFAY 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 312 GHSYLLYGPLACGAMTLMFegvPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRtsLRIMGSVGEPIN 391
Cdd:TIGR02262 217 GLGNALTFPMSVGATTVLM---GERPTPDAVFDRLRRHQPTIFYGVPTLYAAMLADPNLPSEDQVR--LRLCTSAGEALP 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 392 PEAWEWYYNKIGngqCPIVDTWWQTETGGFMITPLPGAteLKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVItdSWP 471
Cdd:TIGR02262 292 AEVGQRWQARFG---VDIVDGIGSTEMLHIFLSNLPGD--VRYGTSGKPVPGYRLRLVGDGGQDVADGEPGELLI--SGP 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 472 GQARTLYGDHDRFEqtyfSTFKGMYF-SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGV 550
Cdd:TIGR02262 365 SSATMYWNNRAKSR----DTFQGEWTrSGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFEIESALIQHPAVLEAAVVGV 440
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 551 P---HNIKGQaiyAYITLNHGEEptpELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:TIGR02262 441 AdedGLIKPK---AFVVLRPGQT---ALETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQRFKLRE 504
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
91-613 |
2.57e-54 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 191.97 E-value: 2.57e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIfggfSPDA 170
Cdd:cd05930 2 DAVAVVDGD------QSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPL----DPSY 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 171 VAGRII----DSNAKLVITAdegmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsA 246
Cdd:cd05930 72 PAERLAyileDSGAKLVLTD-----------------------------------------------------------P 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 247 DCPAeemnaedplFILYTSGSTGKPKGVVHTTGGYLVYAAmTFKYVFDYHPGDIYWCTA----DVGWVTghsylLYGPLA 322
Cdd:cd05930 93 DDLA---------YVIYTSGSTGKPKGVMVEHRGLVNLLL-WMQEAYPLTPGDRVLQFTsfsfDVSVWE-----IFGALL 157
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 323 CGAmTLMF--EGVPNYPGvnRLGQVIDKHQVNILYTAPTAIRALMAEGDKAiegtKRTSLRIMGSVGEPINPEAWEWYYN 400
Cdd:cd05930 158 AGA-TLVVlpEEVRKDPE--ALADLLAEEGITVLHLTPSLLRLLLQELELA----ALPSLRLVLVGGEALPPDLVRRWRE 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 401 KIGNGQcpIVDTWWQTETGGFMITPLPGATELKAGSAT--RPFFGVQPALVDNLGNPQ-EGAsEGNLVITDswPGQARTL 477
Cdd:cd05930 231 LLPGAR--LVNLYGPTEATVDATYYRVPPDDEEDGRVPigRPIPNTRVYVLDENLRPVpPGV-PGELYIGG--AGLARGY 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 478 YGDHD----RFEQTYFSTFKGMYFSGDGARRDEDG--YYwiTGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVP 551
Cdd:cd05930 306 LNRPEltaeRFVPNPFGPGERMYRTGDLVRWLPDGnlEF--LGRIDDQVKIRGYRIELGEIEAALLAHPGVREAAVVARE 383
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1206237686 552 HNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRR 613
Cdd:cd05930 384 DGDGEKRLVAYVVPDEGGELDEE---ELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRK 442
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
108-616 |
6.22e-54 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 190.77 E-value: 6.22e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLK-KLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVhsvifggfspdavagriidsnaklvitA 186
Cdd:cd05958 11 WTYRDLLALANRIANVLVgELGIVPGNRVLLRGSNSPELVACWFGIQKAGAI---------------------------A 63
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 187 DEGMRAGRAIPLKKNVDDALKNPAItsinnvvvfqrtgktgywqegrdlwwhdlivgvsadCPAEEMNAEDPLFILYTSG 266
Cdd:cd05958 64 VATMPLLRPKELAYILDKARITVAL------------------------------------CAHALTASDDICILAFTSG 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 267 STGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEG-VPNypgvnRLGQV 345
Cdd:cd05958 108 TTGAPKATMHFHRDPLASADRYAVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEEaTPD-----LLLSA 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 346 IDKHQVNILYTAPTAIRALMAEGDKAieGTKRTSLRIMGSVGEPINPEAWEWYYNKIGngqCPIVDTWWQTETGGFMITP 425
Cdd:cd05958 183 IARYKPTVLFTAPTAYRAMLAHPDAA--GPDLSSLRKCVSAGEALPAALHRAWKEATG---IPIIDGIGSTEMFHIFISA 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 426 LPGatELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVItdswpgQARTLY-GDHDRFEQTYFStfKGMYFSGDGARR 504
Cdd:cd05958 258 RPG--DARPGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAV------RGPTGCrYLADKRQRTYVQ--GGWNITGDTYSR 327
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 505 DEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVR 584
Cdd:cd05958 328 DPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVLARELQDHAK 407
|
490 500 510
....*....|....*....|....*....|..
gi 1206237686 585 KEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd05958 408 AHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
82-617 |
1.65e-53 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 191.66 E-value: 1.65e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEGDDpsqskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:PRK07656 11 LARAARRFGDKEAYVFGDQR------LTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVP 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVITADEgmragrAIPLKKNVDDALknPAITSInnVVVFQRTGKTGYWQEGRdlwWHDLI 241
Cdd:PRK07656 85 LNTRYTADEAAYILARGDAKALFVLGL------FLGVDYSATTRL--PALEHV--VICETEEDDPHTEKMKT---FTDFL 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 242 VGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVhTTGGYLVYAAMTFKYVFDYHPGDIYWCTA--------DVGWVTgh 313
Cdd:PRK07656 152 AAGDPAERAPEVDPDDVADILFTSGTTGRPKGAM-LTHRQLLSNAADWAEYLGLTEGDRYLAANpffhvfgyKAGVNA-- 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 314 syllygPLACGAMTLMfegVPNYpGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEgtKRTSLRIMGSVGEPINPE 393
Cdd:PRK07656 229 ------PLMRGATILP---LPVF-DPDEVFRLIETERITVLPGPPTMYNSLLQHPDRSAE--DLSSLRLAVTGAASMPVA 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 394 AWEWYYNKIGngqCPIVDTWWQ-TETGGFM-ITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDswP 471
Cdd:PRK07656 297 LLERFESELG---VDIVLTGYGlSEASGVTtFNRLDDDRKTVAGTIGTAIAGVENKIVNELGEEVPVGEVGELLVRG--P 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 472 GQARTLYGDHDRFEQTYfsTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVP 551
Cdd:PRK07656 372 NVMKGYYDDPEATAAAI--DADGWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGVP 449
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 552 HNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:PRK07656 450 DERLGEVGKAYVVLKPGAELTEE---ELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALRE 512
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
98-651 |
4.05e-46 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 173.22 E-value: 4.05e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 98 EGDDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSvIFGGFSPDAVAGRIID 177
Cdd:PRK07529 49 DADPLDRPETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWGGEAAGIANP-INPLLEPEQIAELLRA 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 178 SNAKLVIT------ADEGMRAGRAIPLKKNVDDALK---NPAITSINNVVVFQRTGKTGywqeGRDLWWHDLIVGVSADC 248
Cdd:PRK07529 128 AGAKVLVTlgpfpgTDIWQKVAEVLAALPELRTVVEvdlARYLPGPKRLAVPLIRRKAH----ARILDFDAELARQPGDR 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 249 --PAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAm 326
Cdd:PRK07529 204 lfSGRPIGPDDVAAYFHTGGTTGMPKLAQHTHGN-EVANAWLGALLLGLGPGDTVFCGLPLFHVNALLVTGLAPLARGA- 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 327 TLMFEGVPNY--PGV-NRLGQVIDKHQVNILYTAPTAIRALMaegDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIG 403
Cdd:PRK07529 282 HVVLATPQGYrgPGViANFWKIVERYRINFLSGVPTVYAALL---QVPVDGHDISSLRYALCGAAPLPVEVFRRFEAATG 358
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 404 ngqCPIVDTWWQTE-TGGFMITPLPGatELKAGSATRPFFG--VQPALVDNLGN-PQEGA--SEGNLVItdSWPGQART- 476
Cdd:PRK07529 359 ---VRIVEGYGLTEaTCVSSVNPPDG--ERRIGSVGLRLPYqrVRVVILDDAGRyLRDCAvdEVGVLCI--AGPNVFSGy 431
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 477 LYGDHDR---FEQTYFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHN 553
Cdd:PRK07529 432 LEAAHNKglwLEDGWLNT-------GDLGRIDADGYFWLTGRAKDLIIRGGHNIDPAAIEEALLRHPAVALAAAVGRPDA 504
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 554 IKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIG-PIATPDILHWTDSLPKTRSGKIMRRILRKIAAGDTSNLGDTSTL 632
Cdd:PRK07529 505 HAGELPVAYVQLKPGASATEA---ELLAFARDHIAeRAAVPKHVRILDALPKTAVGKIFKPALRRDAIRRVLRAALRDAG 581
|
570
....*....|....*....
gi 1206237686 633 ADPSVVEKLLEEKQSMQAT 651
Cdd:PRK07529 582 VEAEVVDVVEDGRRGLVAQ 600
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
82-624 |
4.87e-46 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 171.39 E-value: 4.87e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEGDDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:PRK13295 30 LDACVASCPDKTAVTAVRLGTGAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVLNP 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVITAD-------EGMRAGraiplkknvddaLKnPAITSINNVVV--------FQRTGKT 226
Cdd:PRK13295 110 LMPIFRERELSFMLKHAESKVLVVPKtfrgfdhAAMARR------------LR-PELPALRHVVVvggdgadsFEALLIT 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 227 GYWQEGRDLwwhdlIVGVSADCPaeemNAEDPLFILYTSGSTGKPKGVVHTT----GGYLVYAAMtfkyvFDYHPGDIYW 302
Cdd:PRK13295 177 PAWEQEPDA-----PAILARLRP----GPDDVTQLIYTSGTTGEPKGVMHTAntlmANIVPYAER-----LGLGADDVIL 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 303 CTADVGWVTGHSYLLYGPLACGAmTLMFEGVPNypgVNRLGQVIDKHQVNilYT-APTAIRALMAEGDKAiEGTKRTSLR 381
Cdd:PRK13295 243 MASPMAHQTGFMYGLMMPVMLGA-TAVLQDIWD---PARAAELIRTEGVT--FTmASTPFLTDLTRAVKE-SGRPVSSLR 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 382 IMGSVGEPINP----EAWEWYYNKIgngqcpiVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVDNLGNPQE 457
Cdd:PRK13295 316 TFLCAGAPIPGalveRARAALGAKI-------VSAWGMTENGAVTLTKLDDPDERASTTDGCPLPGVEVRVVDADGAPLP 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 458 GASEGNLVITdswpgqARTLYGDHDRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALV 537
Cdd:PRK13295 389 AGQIGRLQVR------GCSNFGGYLKRPQLNGTDADGWFDTGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLY 462
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 538 SHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVR-KEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK13295 463 RHPAIAQVAIVAYPDERLGERACAFVVPRPGQSLDFE---EMVEFLKaQKVAKQYIPERLVVRDALPRTPSGKIQKFRLR 539
|
....*...
gi 1206237686 617 KIAAGDTS 624
Cdd:PRK13295 540 EMLRGEDA 547
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
108-617 |
4.96e-46 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 169.10 E-value: 4.96e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITAD 187
Cdd:cd05903 2 LTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVPE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 EgmragraiplkknvddalknpaitsinnvvvFQRTGktgywqegrdlwwhdlivgvsadcPAEEmnAEDPLFILYTSGS 267
Cdd:cd05903 82 R-------------------------------FRQFD------------------------PAAM--PDAVALLLFTSGT 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 268 TGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMT-LMFEGVPNypgvnRLGQVI 346
Cdd:cd05903 105 TGEPKGVMHSHNT-LSASIRQYAERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVvLQDIWDPD-----KALALM 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 347 DKHQVNILYTAPTAIRALMAEGDKAieGTKRTSLRIMGSVGEPINP----EAWEwyynkigNGQCPIVDTWWQTETGGFM 422
Cdd:cd05903 179 REHGVTFMMGATPFLTDLLNAVEEA--GEPLSRLRTFVCGGATVPRslarRAAE-------LLGAKVCSAYGSTECPGAV 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 423 ITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDswpgqARTLYGDHDRFEQTYFSTFKGMYFSGDGA 502
Cdd:cd05903 250 TSITPAPEDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELLSRG-----PSVFLGYLDRPDLTADAAPEGWFRTGDLA 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 503 RRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVrnW 582
Cdd:cd05903 325 RLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGALLTFDELVAY--L 402
|
490 500 510
....*....|....*....|....*....|....*
gi 1206237686 583 VRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:cd05903 403 DRQGVAKQYWPERLVHVDDLPRTPSGKVQKFRLRE 437
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
88-615 |
1.41e-45 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 169.34 E-value: 1.41e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 88 ERGEQTAIIwegdDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFS 167
Cdd:cd05904 17 AHPSRPALI----DAATGRALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLST 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 168 PDAVAGRIIDSNAKLVITADEGmragraipLKKnvddalknpaITSINNVVVFQRTGKTGYWQEGRDLWWHDlivgvSAD 247
Cdd:cd05904 93 PAEIAKQVKDSGAKLAFTTAEL--------AEK----------LASLALPVVLLDSAEFDSLSFSDLLFEAD-----EAE 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 248 CPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGylvYAAMTFKYVFD----YHPGDIYWCTADVGWVTGHSYLLYGPLAC 323
Cdd:cd05904 150 PPVVVIKQDDVAALLYSSGTTGRSKGVMLTHRN---LIAMVAQFVAGegsnSDSEDVFLCVLPMFHIYGLSSFALGLLRL 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 324 GAMTLMfegVPNYPGVNRLGqVIDKHQVNILYTAPTAIRAlMAEGDKAIEGTKRtSLRIMGSVGEPINPEAWEWYYNKIG 403
Cdd:cd05904 227 GATVVV---MPRFDLEELLA-AIERYKVTHLPVVPPIVLA-LVKSPIVDKYDLS-SLRQIMSGAAPLGKELIEAFRAKFP 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 404 NgqCPIVDTWWQTE-TGGFMITPLPGATELKAGSATRPFFGVQPALVDnlgnPQEGASEGnlvitdswPGQARTLYGDHD 482
Cdd:cd05904 301 N--VDLGQGYGMTEsTGVVAMCFAPEKDRAKYGSVGRLVPNVEAKIVD----PETGESLP--------PNQTGELWIRGP 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 483 RFEQTYFS---------TFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHN 553
Cdd:cd05904 367 SIMKGYLNnpeataatiDKEGWLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDE 446
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1206237686 554 IKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd05904 447 EAGEVPMAFVVRKPGSSLTED---EIMDFVAKQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
104-617 |
3.39e-45 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 168.42 E-value: 3.39e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 104 QSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLV 183
Cdd:TIGR03098 22 HDRTLTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPINPLLKAEQVAHILADCNVRLL 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 184 ITADEGMRAgraiplkknVDDALknPAITSINNVVVFQRTGKTGYWQEGRD-LWWHDLIVGVSADcPAEEMNAEDPLFIL 262
Cdd:TIGR03098 102 VTSSERLDL---------LHPAL--PGCHDLRTLIIVGDPAHASEGHPGEEpASWPKLLALGDAD-PPHPVIDSDMAAIL 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 263 YTSGSTGKPKGVV--HTTggyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMfegvpNYPGVN 340
Cdd:TIGR03098 170 YTSGSTGRPKGVVlsHRN---LVAGAQSVATYLENRPDDRLLAVLPLSFDYGFNQLTTAFYVGATVVLH-----DYLLPR 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 341 RLGQVIDKHQVNILYTAPtAIRALMAEGDKAIEGTKrtSLRIMGSVGEPINPEAWEWYYNKIGNGQcpIVDTWWQTEtgG 420
Cdd:TIGR03098 242 DVLKALEKHGITGLAAVP-PLWAQLAQLDWPESAAP--SLRYLTNSGGAMPRATLSRLRSFLPNAR--LFLMYGLTE--A 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 421 FMITPL-PGATELKAGSATRPFFGVQPALVDNLGN------PQEGASEGNLVITDSWPGQARTlygdHDRFE----QTYF 489
Cdd:TIGR03098 315 FRSTYLpPEEVDRRPDSIGKAIPNAEVLVLREDGSecapgeEGELVHRGALVAMGYWNDPEKT----AERFRplppFPGE 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 490 STFKGM-YFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHG 568
Cdd:TIGR03098 391 LHLPELaVWSGDTVRRDEEGFLYFVGRRDEMIKTSGYRVSPTEVEEVAYATGLVAEAVAFGVPDPTLGQAIVLVVTPPGG 470
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 1206237686 569 EEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:TIGR03098 471 EELDRA---ALLAECRARLPNYMVPALIHVRQALPRNANGKIDRKALAK 516
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
82-617 |
2.97e-44 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 165.88 E-value: 2.97e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEGDDPSQskHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:cd12119 2 LEHAARLHGDREIVSRTHEGEVH--RYTYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHT 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVITaDEGMragraIPLKKNVDDALKNpaitsINNVVVFqrTGKTGYWQEGRDLWWH--D 239
Cdd:cd12119 80 INPRLFPEQIAYIINHAEDRVVFV-DRDF-----LPLLEAIAPRLPT-----VEHVVVM--TDDAAMPEPAGVGVLAyeE 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 240 LIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTF--KYVFDYHPGDIY-----------WCTAD 306
Cdd:cd12119 147 LLAAESPEYDWPDFDENTAAAICYTSGTTGNPKGVVYSHRS-LVLHAMAAllTDGLGLSESDVVlpvvpmfhvnaWGLPY 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 307 VGWVTGHSYLLYGPLACGAMtlmfegvpnypgvnrLGQVIDKHQVNILYTAPTAIRALMAEGDKaiEGTKRTSLR---IM 383
Cdd:cd12119 226 AAAMVGAKLVLPGPYLDPAS---------------LAELIEREGVTFAAGVPTVWQGLLDHLEA--NGRDLSSLRrvvIG 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 384 GSVGEPINPEAWEWYYnkigngqCPIVDTWWQTETG--GFMITPLPGATELKAG-------SATRPFFGVQPALVDNLGN 454
Cdd:cd12119 289 GSAVPRSLIEAFEERG-------VRVIHAWGMTETSplGTVARPPSEHSNLSEDeqlalraKQGRPVPGVELRIVDDDGR 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 455 --PQEGASEGNLVITDSWpgQARTLYGDHDR----FEQTYFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGHRLG 528
Cdd:cd12119 362 elPWDGKAVGELQVRGPW--VTKSYYKNDEEsealTEDGWLRT-------GDVATIDEDGYLTITDRSKDVIKSGGEWIS 432
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 529 TAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSG 608
Cdd:cd12119 433 SVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGATVTAE---ELLEFLADKVAKWWLPDDVVFVDEIPKTSTG 509
|
....*....
gi 1206237686 609 KIMRRILRK 617
Cdd:cd12119 510 KIDKKALRE 518
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
109-547 |
8.01e-44 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 162.05 E-value: 8.01e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKL-GVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITaD 187
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLT-D 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 EGMRAgraiplkknvDDALKNPAITSInnvvvfqrtgktgywqegRDLWWHDLIVGVSADCPAEEMNAEDPLFILYTSGS 267
Cdd:TIGR01733 80 SALAS----------RLAGLVLPVILL------------------DPLELAALDDAPAPPPPDAPSGPDDLAYVIYTSGS 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 268 TGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTA----DVgwvtghSYL-LYGPLACGAMTLMFEGVPNYPGVNRL 342
Cdd:TIGR01733 132 TGRPKGVVVTHRS-LVNLLAWLARRYGLDPDDRVLQFAslsfDA------SVEeIFGALLAGATLVVPPEDEERDDAALL 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 343 GQVIDKHQVNILYTAPTAIRALMAEGDKAIegtkrTSLRIMGSVGEPINPEA---WEWYYnkignGQCPIVDTWWQTE-T 418
Cdd:TIGR01733 205 AALIAEHPVTVLNLTPSLLALLAAALPPAL-----ASLRLVILGGEALTPALvdrWRARG-----PGARLINLYGPTEtT 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 419 GGFMITPLPGATELKAGSAT--RPFFGVQPALVDNLGNPQEGASEGNLVItdSWPGQARTLYGDHD----RFEQTYFSTF 492
Cdd:TIGR01733 275 VWSTATLVDPDDAPRESPVPigRPLANTRLYVLDDDLRPVPVGVVGELYI--GGPGVARGYLNRPEltaeRFVPDPFAGG 352
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*..
gi 1206237686 493 KG--MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAV 547
Cdd:TIGR01733 353 DGarLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
82-615 |
3.06e-42 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 159.75 E-value: 3.06e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:cd17646 4 VAEQAARTPDAPAVVDEG------RTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVITadegmRAGRAIPLKKNVDDALKNPAITSinnvvvfqrtgktgywqegrdlwwhdli 241
Cdd:cd17646 78 LDPGYPADRLAYMLADAGPAVVLT-----TADLAARLPAGGDVALLGDEALA---------------------------- 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 242 vGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADVGW-VTGhsYLLYGP 320
Cdd:cd17646 125 -APPATPPLVPPRPDNLAYVIYTSGSTGRPKGVMVTHAG-IVNRLLWMQDEYPLGPGDRVLQKTPLSFdVSV--WELFWP 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 321 LACGAMTLMFEgvpnyPGVNR----LGQVIDKHQVNILYTAPTAIRALMAEGDkaieGTKRTSLRIMGSVGEPINPEAWE 396
Cdd:cd17646 201 LVAGARLVVAR-----PGGHRdpayLAALIREHGVTTCHFVPSMLRVFLAEPA----AGSCASLRRVFCSGEALPPELAA 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 397 WY--------YNKIGNGQCPIVDTWWQTeTGGFMITPLP-GatelkagsatRPFFGVQPALVDNLGNPQEGASEGNLVIt 467
Cdd:cd17646 272 RFlalpgaelHNLYGPTEAAIDVTHWPV-RGPAETPSVPiG----------RPVPNTRLYVLDDALRPVPVGVPGELYL- 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 468 dswpGQARTLYGDHDRFEQTY-------FSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHP 540
Cdd:cd17646 340 ----GGVQLARGYLGRPALTAerfvpdpFGPGSRMYRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHP 415
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1206237686 541 KIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElyTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd17646 416 AVTHAVVVARAAPAGAARLVGYVVPAAGAAGPDT--AALRAHLAERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
139-616 |
6.37e-42 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 158.83 E-value: 6.37e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 139 MPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADEGMRAGRAIPLKKNVDDAlkNPAitsinNVV 218
Cdd:PLN03051 1 MPMTVDAVIIYLAIVLAGCVVVSVADSFSAKEIATRLDISGAKGVFTQDVVLRGGRALPLYSKVVEA--APA-----KAI 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 219 VFQRTGKTGYWQ-EGRDLWWHD-LIVGVSADCPAEE------MNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFK 290
Cdd:PLN03051 74 VLPAAGEPVAVPlREQDLSWCDfLGVAAAQGSVGGNeyspvyAPVESVTNILFSSGTTGEPKAIPWTHLSPLRCASDGWA 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 291 YVfDYHPGDIYWCTADVGWVTGhSYLLYGPLACGAMTLMFEGVPNYPGvnrLGQVIDKHQVNILYTAPTAIRALMAEGDK 370
Cdd:PLN03051 154 HM-DIQPGDVVCWPTNLGWMMG-PWLLYSAFLNGATLALYGGAPLGRG---FGKFVQDAGVTVLGLVPSIVKAWRHTGAF 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 371 AIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNGQcPIVDTWWQTETGGFMI--TPL-PGAtelkAGSATRPFFGVQPA 447
Cdd:PLN03051 229 AMEGLDWSKLRVFASTGEASAVDDVLWLSSVRGYYK-PVIEYCGGTELASGYIssTLLqPQA----PGAFSTASLGTRFV 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 448 LVDNLGNPQ--EGASEGNLVITDSWPGQA-RTLYGDHDrfeQTYfstFKGM--YFS--------GDGARRDEDGYYWITG 514
Cdd:PLN03051 304 LLNDNGVPYpdDQPCVGEVALAPPMLGASdRLLNADHD---KVY---YKGMpmYGSkgmplrrhGDIMKRTPGGYFCVQG 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 515 RVDDVLNVSGHRLGTAEIESALVSHPK-IAEAAVVGVPhNIKGQAIYAYITLNHGEEPT-------PELYTEVRNWVRKE 586
Cdd:PLN03051 378 RADDTMNLGGIKTSSVEIERACDRAVAgIAETAAVGVA-PPDGGPELLVIFLVLGEEKKgfdqarpEALQKKFQEAIQTN 456
|
490 500 510
....*....|....*....|....*....|
gi 1206237686 587 IGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PLN03051 457 LNPLFKVSRVKIVPELPRNASNKLLRRVLR 486
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
91-615 |
6.46e-42 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 157.85 E-value: 6.46e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWEGDdpsqskHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDA 170
Cdd:cd17643 2 EAVAVVDEDR------RLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVER 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 171 VAGRIIDSNAKLVITadegmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpa 250
Cdd:cd17643 76 IAFILADSGPSLLLT----------------------------------------------------------------- 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 251 eemNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTfKYVFDYHPGDIywctadvgWVTGHSYL-------LYGPLAC 323
Cdd:cd17643 91 ---DPDDLAYVIYTSGSTGRPKGVVVSHANVLALFAAT-QRWFGFNEDDV--------WTLFHSYAfdfsvweIWGALLH 158
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 324 GAMTLMfegVPNypGVNR----LGQVIDKHQVNILYTAPTAIRALMAEGDKaiEGTKRTSLR--IMGsvGEPINPEAWEW 397
Cdd:cd17643 159 GGRLVV---VPY--EVARspedFARLLRDEGVTVLNQTPSAFYQLVEAADR--DGRDPLALRyvIFG--GEALEAAMLRP 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 398 YYNKIGNGQCPIVDTWWQTETGGFM-ITPLpGATELKAGSAT---RPFFGVQPALVDNLGNPQEGASEGNLVItdSWPGQ 473
Cdd:cd17643 230 WAGRFGLDRPQLVNMYGITETTVHVtFRPL-DAADLPAAAASpigRPLPGLRVYVLDADGRPVPPGVVGELYV--SGAGV 306
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 474 ARtlyGDHDRFEQT--YF--STFKG----MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEA 545
Cdd:cd17643 307 AR---GYLGRPELTaeRFvaNPFGGpgsrMYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDA 383
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 546 AVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd17643 384 AVIVREDEPGDTRLVAYVVADDGAAADIA---ELRALLKELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
84-616 |
3.13e-41 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 156.74 E-value: 3.13e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 84 RHLTERGEQTAIIWEGDdpsqskHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIF 163
Cdd:cd17651 3 RQAARTPDAPALVAEGR------RLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLD 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 164 GGFSPDAVAGRIIDSNAKLVITADEgmRAGRAIPlkknvddalknpaitsinnvvvfqrtgktgywqEGRDLWWHDLIVG 243
Cdd:cd17651 77 PAYPAERLAFMLADAGPVLVLTHPA--LAGELAV---------------------------------ELVAVTLLDQPGA 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 244 VSADC--PAEEMNAEDPLFILYTSGSTGKPKGVV--HTTGGYLVYAAmtfKYVFDYHPGDIYWCTADVGW-VTGHSylLY 318
Cdd:cd17651 122 AAGADaePDPALDADDLAYVIYTSGSTGRPKGVVmpHRSLANLVAWQ---ARASSLGPGARTLQFAGLGFdVSVQE--IF 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 319 GPLACGAmTLMFEGVPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAieGTKRTSLRIMGSVGEP--INPEAWE 396
Cdd:cd17651 197 STLCAGA-TLVLPPEEVRTDPPALAAWLDEQRISRVFLPTVALRALAEHGRPL--GVRLAALRYLLTGGEQlvLTEDLRE 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 397 WY--------YNKIGNGQCPIVDTWWQT-ETGGFMITPLPGatelkagsatRPFFGVQPALVDNLGNPQEGASEGNLVIt 467
Cdd:cd17651 274 FCaglpglrlHNHYGPTETHVVTALSLPgDPAAWPAPPPIG----------RPIDNTRVYVLDAALRPVPPGVPGELYI- 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 468 dSWPGQARTLYGD----HDRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIA 543
Cdd:cd17651 343 -GGAGLARGYLNRpeltAERFVPDPFVPGARMYRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVR 421
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1206237686 544 EAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd17651 422 EAVVLAREDRPGEKRLVAYVVGDPEAPVDAA---ELRAALATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
91-616 |
1.43e-40 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 154.06 E-value: 1.43e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVhsviFGGFSPDA 170
Cdd:cd17649 2 DAVALVFGD------QSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGA----YVPLDPEY 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 171 VAGR----IIDSNAKLVITadegmragraiplkknvddalknpaitsinnvvvfqrtgktgywQEGRDLWWhdlivgvsa 246
Cdd:cd17649 72 PAERlrymLEDSGAGLLLT--------------------------------------------HHPRQLAY--------- 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 247 dcpaeemnaedplfILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYvFDYHPGDIYWCTADVGWVTGHSYLlYGPLACGAm 326
Cdd:cd17649 99 --------------VIYTSGSTGTPKGVAVSHGPLAAHCQATAER-YGLTPGDRELQFASFNFDGAHEQL-LPPLICGA- 161
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 327 TLMFEGVPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGtKRTSLRIMGSVGEPINPEAWEwyynKIGNGQ 406
Cdd:cd17649 162 CVVLRPDELWASADELAEMVRELGVTVLDLPPAYLQQLAEEADRTGDG-RPPSLRLYIFGGEALSPELLR----RWLKAP 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 407 CPIVDTWWQTETggfMITPL--PGATELKAGSAT----RPFFGVQPALVDNLGNPQEGASEGNLVITDswPGQARtlyGD 480
Cdd:cd17649 237 VRLFNAYGPTEA---TVTPLvwKCEAGAARAGASmpigRPLGGRSAYILDADLNPVPVGVTGELYIGG--EGLAR---GY 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 481 HDRFEQT--------YFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPH 552
Cdd:cd17649 309 LGRPELTaerfvpdpFGAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDG 388
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1206237686 553 NIkGQAIYAYITLNHGEEpTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd17649 389 AG-GKQLVAYVVLRAAAA-QPELRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
91-615 |
2.92e-40 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 155.12 E-value: 2.92e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLK-KLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPD 169
Cdd:PRK08314 25 DKTAIVFYG------RAISYRELLEEAERLAGYLQqECGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPMNREE 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 170 AVAGRIIDSNAKLVIT----ADEGMRAGRAIPLKK----NVDDALKNPAITSINNVVVFQRTGKTGywQEGRDLWWHD-L 240
Cdd:PRK08314 99 ELAHYVTDSGARVAIVgselAPKVAPAVGNLRLRHvivaQYSDYLPAEPEIAVPAWLRAEPPLQAL--APGGVVAWKEaL 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 241 IVGVSAdcPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGP 320
Cdd:PRK08314 177 AAGLAP--PPHTAGPDDLAVLPYTSGTTGVPKGCMHTHRT-VMANAVGSVLWSNSTPESVVLAVLPLFHVTGMVHSMNAP 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 321 LACGA-MTLMfegvpnyPGVNR--LGQVIDKHQVNILYTAPTAIRALMAEGDkaIEGTKRTSLRIMGSVGEPInPEAwew 397
Cdd:PRK08314 254 IYAGAtVVLM-------PRWDReaAARLIERYRVTHWTNIPTMVVDFLASPG--LAERDLSSLRYIGGGGAAM-PEA--- 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 398 yynkIGN---GQC--PIVDTWWQTETGGFMITPLPGATELK-AGSatrPFFGVQPALVDnlgnPQEGA-----SEGNLVI 466
Cdd:PRK08314 321 ----VAErlkELTglDYVEGYGLTETMAQTHSNPPDRPKLQcLGI---PTFGVDARVID----PETLEelppgEVGEIVV 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 467 tdSWPGQARTLYGDHDRFEQTyFSTFKGMYF--SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAE 544
Cdd:PRK08314 390 --HGPQVFKGYWNRPEATAEA-FIEIDGKRFfrTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQE 466
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 545 AAVVGVPHNIKGQAIYAYITLNHGEEPTPElYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:PRK08314 467 ACVIATPDPRRGETVKAVVVLRPEARGKTT-EEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKILWRQL 536
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
88-615 |
1.27e-39 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 151.97 E-value: 1.27e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 88 ERGEQTAIIWEGDDpsqskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFS 167
Cdd:cd12117 9 RTPDAVAVVYGDRS------LTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPELP 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 168 PDAVAGRIIDSNAKLVITaDEGMrAGRAIPLKKNVDDALKNPAitsinnvvvfqrtgktgywqegrdlwwhdlivgVSAD 247
Cdd:cd12117 83 AERLAFMLADAGAKVLLT-DRSL-AGRAGGLEVAVVIDEALDA---------------------------------GPAG 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 248 CPAEEMNAEDPLFILYTSGSTGKPKGV----------VHTTGgylvYAAMTfkyvfdyhPGDIYWCTADVGWvTGHSYLL 317
Cdd:cd12117 128 NPAVPVSPDDLAYVMYTSGSTGRPKGVavthrgvvrlVKNTN----YVTLG--------PDDRVLQTSPLAF-DASTFEI 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 318 YGPLACGA-MTLMFEGVPNYPgvNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGtkrtsLRIMGSVGEPINPEAwe 396
Cdd:cd12117 195 WGALLNGArLVLAPKGTLLDP--DALGALIAEEGVTVLWLTAALFNQLADEDPECFAG-----LRELLTGGEVVSPPH-- 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 397 wyYNKIgNGQCP---IVDTWWQTETGGF----MITPL-PGATELKAGsatRPFFGVQPALVDNLGNPQEGASEGNLVItd 468
Cdd:cd12117 266 --VRRV-LAACPglrLVNGYGPTENTTFttshVVTELdEVAGSIPIG---RPIANTRVYVLDEDGRPVPPGVPGELYV-- 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 469 SWPGQARTLYGDHD----RFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAE 544
Cdd:cd12117 338 GGDGLALGYLNRPAltaeRFVADPFGPGERLYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVRE 417
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 545 AAVVGVPHNIKGQAIYAYITLnhGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd12117 418 AVVVVREDAGGDKRLVAYVVA--EGALDAA---ELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
90-616 |
5.48e-39 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 150.99 E-value: 5.48e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 90 GEQTAIIWEgDDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPD 169
Cdd:PRK08008 21 GHKTALIFE-SSGGVVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLRE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 170 AVAGRIIDSNAKLVITADEGMRAGRAIplKKNVDDALKNPAIT-----SINNVVVFQRTGKtgywQEGRDLwwhDLIVGV 244
Cdd:PRK08008 100 ESAWILQNSQASLLVTSAQFYPMYRQI--QQEDATPLRHICLTrvalpADDGVSSFTQLKA----QQPATL---CYAPPL 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 245 SADCPAEemnaedplfILYTSGSTGKPKGVVHTT-----GGYL-----------VYAAMTFKYVFDYHpgdiywCTAdvg 308
Cdd:PRK08008 171 STDDTAE---------ILFTSGTTSRPKGVVITHynlrfAGYYsawqcalrdddVYLTVMPAFHIDCQ------CTA--- 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 309 wvtghsylLYGPLACGAMTLMFEgvpNYPGVNRLGQVIdKHQVNILYTAPTAIRALMAEgdKAIEGTKRTSLRIMG---- 384
Cdd:PRK08008 233 --------AMAAFSAGATFVLLE---KYSARAFWGQVC-KYRATITECIPMMIRTLMVQ--PPSANDRQHCLREVMfyln 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 385 -SVGEPinpEAWEWYYNkigngqCPIVDTWWQTETGGFMITPLPGAtELKAGSATRPFFGVQPALVDNLGNPQEGASEGN 463
Cdd:PRK08008 299 lSDQEK---DAFEERFG------VRLLTSYGMTETIVGIIGDRPGD-KRRWPSIGRPGFCYEAEIRDDHNRPLPAGEIGE 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 464 LVITDSwPGqaRTL----YGDHDRFEQTYfsTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSH 539
Cdd:PRK08008 369 ICIKGV-PG--KTIfkeyYLDPKATAKVL--EADGWLHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATH 443
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1206237686 540 PKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK08008 444 PKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEE---EFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNLK 517
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
73-616 |
5.86e-39 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 150.91 E-value: 5.86e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 73 GTLNLSAncLDRHlterGEQTAIIWegDDPSqskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLAC 152
Cdd:PRK06188 15 GHLLVSA--LKRY----PDRPALVL--GDTR----LTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIGAA 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 153 ARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVItADEGMRAGRAIPLKKNVDdalknpaitSINNVVVFQRTGktgywqEG 232
Cdd:PRK06188 83 QLAGLRRTALHPLGSLDDHAYVLEDAGISTLI-VDPAPFVERALALLARVP---------SLKHVLTLGPVP------DG 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 233 RDLWWHDLIVG----VSADCPAeemnaeDPLFILYTSGSTGKPKGVVHTTGGYlvyAAMTfkyvfdyhpgdiYWCTADVG 308
Cdd:PRK06188 147 VDLLAAAAKFGpaplVAAALPP------DIAGLAYTGGTTGKPKGVMGTHRSI---ATMA------------QIQLAEWE 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 309 WVTGHSYLLYGPL--ACGAM---TLMFEG----VPNY-PGvnRLGQVIDKHQVNILYTAPTAIRALMAEGDkaIEGTKRT 378
Cdd:PRK06188 206 WPADPRFLMCTPLshAGGAFflpTLLRGGtvivLAKFdPA--EVLRAIEEQRITATFLVPTMIYALLDHPD--LRTRDLS 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 379 SLRIMGSVGEPINP----EAWEwyynKIGngqcPI-VDTWWQTETGGFmITPLP-----GATELKAGSATRPFFGVQPAL 448
Cdd:PRK06188 282 SLETVYYGASPMSPvrlaEAIE----RFG----PIfAQYYGQTEAPMV-ITYLRkrdhdPDDPKRLTSCGRPTPGLRVAL 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 449 VDNLGNPQEGASEGNLVItdSWPGQARtlyGDHDRFEQTYfSTFKGMYF-SGDGARRDEDGYYWITGRVDDVLNVSGHRL 527
Cdd:PRK06188 353 LDEDGREVAQGEVGEICV--RGPLVMD---GYWNRPEETA-EAFRDGWLhTGDVAREDEDGFYYIVDRKKDMIVTGGFNV 426
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 528 GTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRS 607
Cdd:PRK06188 427 FPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAAVDAA---ELQAHVKERKGSVHAPKQVDFVDSLPLTAL 503
|
....*....
gi 1206237686 608 GKIMRRILR 616
Cdd:PRK06188 504 GKPDKKALR 512
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
93-616 |
2.61e-38 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 148.51 E-value: 2.61e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 93 TAIIWEGDdpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVA 172
Cdd:PRK08276 2 AVIMAPSG-----EVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIA 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 173 GRIIDSNAKLVITADEGMRAGRAIPlkknvdDALKNPAitsinnVVVFQRTGKTGYWQEgrdlwWHDLIVGVSADCPAEE 252
Cdd:PRK08276 77 YIVDDSGAKVLIVSAALADTAAELA------AELPAGV------PLLLVVAGPVPGFRS-----YEEALAAQPDTPIADE 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 253 MNAEDplfILYTSGSTGKPKGV--------VHTTGGylvyaAMTFKYVFDYH--PGDIYWCTADvgwvtghsylLY--GP 320
Cdd:PRK08276 140 TAGAD---MLYSSGTTGRPKGIkrplpgldPDEAPG-----MMLALLGFGMYggPDSVYLSPAP----------LYhtAP 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 321 LACGAMTLMFEGV----PNYPGVNRLgQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEA-- 394
Cdd:PRK08276 202 LRFGMSALALGGTvvvmEKFDAEEAL-ALIERYRVTHSQLVPTMFVRMLKLPEEVRARYDVSSLRVAIHAAAPCPVEVkr 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 395 --WEWYynkignGqcPIVD-TWWQTETGGF-MITPLPGATelKAGSATRPFFGVQPALVDNlGNPQEGASEGNlvITDSW 470
Cdd:PRK08276 281 amIDWW------G--PIIHeYYASSEGGGVtVITSEDWLA--HPGSVGKAVLGEVRILDED-GNELPPGEIGT--VYFEM 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 471 PGQARTLYGDHDRFEQTYFStfKGMYFSGDGARRDEDGYYWITGRVDDVLnVSGhrlGT----AEIESALVSHPKIAEAA 546
Cdd:PRK08276 348 DGYPFEYHNDPEKTAAARNP--HGWVTVGDVGYLDEDGYLYLTDRKSDMI-ISG---GVniypQEIENLLVTHPKVADVA 421
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 547 VVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK08276 422 VFGVPDEEMGERVKAVVQPADGADAGDALAAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLR 491
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
101-615 |
2.76e-38 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 148.43 E-value: 2.76e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 101 DPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNA 180
Cdd:cd05923 22 DPARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKAAELAELIERGEM 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 181 KLVITAD--EGMRA-----GRAIPLKKNVDDALKNPAITSInnvvvfqrtgktgywqegrdlwwhdlivgvsadcPAEEM 253
Cdd:cd05923 102 TAAVIAVdaQVMDAifqsgVRVLALSDLVGLGEPESAGPLI----------------------------------EDPPR 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 254 NAEDPLFILYTSGSTGKPKGVV---HTTGGYLVYAAMTFKYVFDYHpgdiywctadvGWVTGHS--YLLYGPLACGAMTL 328
Cdd:cd05923 148 EPEQPAFVFYTSGTTGLPKGAVipqRAAESRVLFMSTQAGLRHGRH-----------NVVLGLMplYHVIGFFAVLVAAL 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 329 MFEG---VPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAieGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNg 405
Cdd:cd05923 217 ALDGtyvVVEEFDPADALKLIEQERVTSLFATPTHLDALAAAAEFA--GLKLSSLRHVTFAGATMPDAVLERVNQHLPG- 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 406 qcPIVDTWWQTETGGFMITPLPgatelKAGSATRPFFGVQPALVDNLGNPQEGAS---EGNLVITDSwpgQARTLYGDHD 482
Cdd:cd05923 294 --EKVNIYGTTEAMNSLYMRDA-----RTGTEMRPGFFSEVRIVRIGGSPDEALAngeEGELIVAAA---ADAAFTGYLN 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 483 RFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAY 562
Cdd:cd05923 364 QPEATAKKLQDGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTAC 443
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 1206237686 563 ITLNHGEEPTPELYTEVRNwvrKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd05923 444 VVPREGTLSADELDQFCRA---SELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
82-575 |
4.74e-38 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 151.93 E-value: 4.74e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEGDdpsqskHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:COG1020 482 FEAQAARTPDAVAVVFGDQ------SLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVP 555
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IfggfSPDAVAGRII----DSNAKLVITADEgmragraiplkknVDDALKNPAITsinnVVVFQRtgktgywqegrdlww 237
Cdd:COG1020 556 L----DPAYPAERLAymleDAGARLVLTQSA-------------LAARLPELGVP----VLALDA--------------- 599
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 238 hDLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIywctadVGWVTGHS--- 314
Cdd:COG1020 600 -LALAAEPATNPPVPVTPDDLAYVIYTSGSTGRPKGVMVEHRA-LVNLLAWMQRRYGLGPGDR------VLQFASLSfda 671
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 315 --YLLYGPLACGAmTLMF---EGVPNypgVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAiegtkRTSLRIMGSVGEP 389
Cdd:COG1020 672 svWEIFGALLSGA-TLVLappEARRD---PAALAELLARHRVTVLNLTPSLLRALLDAAPEA-----LPSLRLVLVGGEA 742
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 390 INPEAWEWYYNKigNGQCPIVDTWWQTETGGFMITPLPGATELKAGSAT--RPFFGVQPALVDNLGNPQ-EGASeGNLVI 466
Cdd:COG1020 743 LPPELVRRWRAR--LPGARLVNLYGPTETTVDSTYYEVTPPDADGGSVPigRPIANTRVYVLDAHLQPVpVGVP-GELYI 819
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 467 tdSWPGQARtlyGDHDRFEQT--YF----STFKG--MYFSGDGARRDEDG---YywiTGRVDDVLNVSGHR--LGtaEIE 533
Cdd:COG1020 820 --GGAGLAR---GYLNRPELTaeRFvadpFGFPGarLYRTGDLARWLPDGnleF---LGRADDQVKIRGFRieLG--EIE 889
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 1206237686 534 SALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPEL 575
Cdd:COG1020 890 AALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAAL 931
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
108-615 |
8.08e-38 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 145.70 E-value: 8.08e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITAD 187
Cdd:cd05935 2 LTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVGS 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 EgmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemnAEDPLFILYTSGS 267
Cdd:cd05935 82 E------------------------------------------------------------------LDDLALIPYTSGT 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 268 TGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAmTLMFEGVPNYpgvNRLGQVID 347
Cdd:cd05935 96 TGLPKGCMHTHFS-AAANALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGG-TYVLMARWDR---ETALELIE 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 348 KHQVNILYTAPTAIRALMAegDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGngqCPIVDTWWQTETGGFMITPLP 427
Cdd:cd05935 171 KYKVTFWTNIPTMLVDLLA--TPEFKTRDLSSLKVLTGGGAPMPPAVAEKLLKLTG---LRFVEGYGLTETMSQTHTNPP 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 428 GAteLKAGSATRPFFGVQPALVD-NLGNPQEGASEGNLVItdSWPgQARTLYGDHDRFEQTYFSTFKGMYF--SGDGARR 504
Cdd:cd05935 246 LR--PKLQCLGIP*FGVDARVIDiETGRELPPNEVGEIVV--RGP-QIFKGYWNRPEETEESFIEIKGRRFfrTGDLGYM 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 505 DEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGE--EPTPElytEVRNW 582
Cdd:cd05935 321 DEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYrgKVTEE---DIIEW 397
|
490 500 510
....*....|....*....|....*....|...
gi 1206237686 583 VRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd05935 398 AREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
91-615 |
2.37e-37 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 144.70 E-value: 2.37e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWEGDDpsqskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHsvifggfspda 170
Cdd:cd05945 6 DRPAVVEGGRT------LTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAY----------- 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 171 vagriidsnaklvitadegmragraIPLkknvddalkNPAITsinnvvvFQRtgktgyWQEgrdlwwhdlIVGVSAdcPA 250
Cdd:cd05945 69 -------------------------VPL---------DASSP-------AER------IRE---------ILDAAK--PA 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 251 EEMNAEDPLF-ILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYvFDYHPGDIYWCTADVGW---VTGhsylLYGPLACGAm 326
Cdd:cd05945 91 LLIADGDDNAyIIFTSGSTGRPKGVQISHDNLVSFTNWMLSD-FPLGPGDVFLNQAPFSFdlsVMD----LYPALASGA- 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 327 TLMFegVPN--YPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKrtSLRIMGSVGEPI-NPEAWEWyynKIG 403
Cdd:cd05945 165 TLVP--VPRdaTADPKQLFRFLAEHGITVWVSTPSFAAMCLLSPTFTPESLP--SLRHFLFCGEVLpHKTARAL---QQR 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 404 NGQCPIVDTWWQTET----GGFMITPLP--GATELKAGsatRPFFGVQPALVDNLGNPQEGASEGNLVITdswpGQARTL 477
Cdd:cd05945 238 FPDARIYNTYGPTEAtvavTYIEVTPEVldGYDRLPIG---YAKPGAKLVILDEDGRPVPPGEKGELVIS----GPSVSK 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 478 -Y----GDHDRFeqtyFSTFKGM--YFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGV 550
Cdd:cd05945 311 gYlnnpEKTAAA----FFPDEGQraYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPK 386
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1206237686 551 PHNIKGQAIYAYITLNHGEEPTPElyTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd05945 387 YKGEKVTELIAFVVPKPGAEAGLT--KAIKAELAERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
257-617 |
3.26e-37 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 144.35 E-value: 3.26e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 257 DPLFILYTSGSTGKPKGVVHTTGgylVYAAMTFKYVFDYHpgdiyWCTADV-----------GWVTGhsylLYGPLACGA 325
Cdd:cd05941 90 DPALILYTSGTTGRPKGVVLTHA---NLAANVRALVDAWR-----WTEDDVllhvlplhhvhGLVNA----LLCPLFAGA 157
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 326 mTLMFEGVPNYPGVNrlgQVIDKHQVNILYTAPT-------AIRALMAEGDKAIEGTKRtSLRIMGSVGEPINPEAWEWY 398
Cdd:cd05941 158 -SVEFLPKFDPKEVA---ISRLMPSITVFMGVPTiytrllqYYEAHFTDPQFARAAAAE-RLRLMVSGSAALPVPTLEEW 232
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 399 YNKIGNgqcPIVDTWWQTETGGFMITPLPGatELKAGSATRPFFGVQPALVDNLGN-PQEGASEGNL------VITDSWP 471
Cdd:cd05941 233 EAITGH---TLLERYGMTEIGMALSNPLDG--ERRPGTVGMPLPGVQARIVDEETGePLPRGEVGEIqvrgpsVFKEYWN 307
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 472 GQARTlygdHDRF-EQTYFSTfkgmyfsGDGARRDEDGYYWITGRV-DDVLNVSGHRLGTAEIESALVSHPKIAEAAVVG 549
Cdd:cd05941 308 KPEAT----KEEFtDDGWFKT-------GDLGVVDEDGYYWILGRSsVDIIKSGGYKVSALEIERVLLAHPGVSECAVIG 376
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 550 VPHNIKGQAIYAYITLNHGEEP-TPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:cd05941 377 VPDPDWGERVVAVVVLRAGAAAlSLE---ELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
108-615 |
5.37e-37 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 144.36 E-value: 5.37e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITad 187
Cdd:cd12116 13 LSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYILEDAEPALVLT-- 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 egmragraiplkknvDDALKNPAITSInnvvvfqrtgktgywqegrDLWWHDLI-VGVSADCPAEEMNAEDPLFILYTSG 266
Cdd:cd12116 91 ---------------DDALPDRLPAGL-------------------PVLLLALAaAAAAPAAPRTPVSPDDLAYVIYTSG 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 267 STGKPKGVVHTTGGYLVY-AAMTFKyvFDYHPGD----IYWCTADVgwvtghSYL-LYGPLACGAMTLMFEGVPNYpGVN 340
Cdd:cd12116 137 STGRPKGVVVSHRNLVNFlHSMRER--LGLGPGDrllaVTTYAFDI------SLLeLLLPLLAGARVVIAPRETQR-DPE 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 341 RLGQVIDKHQVNILYTAPTAIRALMAEGdkaieGTKRTSLRIM-GsvGEPINPEAWEWYYNKIGngqcpivdTWWQ---- 415
Cdd:cd12116 208 ALARLIEAHSITVMQATPATWRMLLDAG-----WQGRAGLTALcG--GEALPPDLAARLLSRVG--------SLWNlygp 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 416 TETggfmiTPLPGATELKAGSAT----RPFFGVQPALVDNLGNPQEGASEGNLVITDswPGQARTLYGDHD----RFEQT 487
Cdd:cd12116 273 TET-----TIWSTAARVTAAAGPipigRPLANTQVYVLDAALRPVPPGVPGELYIGG--DGVAQGYLGRPAltaeRFVPD 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 488 YFSTFKG-MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQaIYAYITLN 566
Cdd:cd12116 346 PFAGPGSrLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVVREDGGDRR-LVAYVVLK 424
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 1206237686 567 HGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd12116 425 AGAAPDAA---ALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
103-615 |
8.87e-37 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 143.23 E-value: 8.87e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 103 SQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKL 182
Cdd:cd12115 20 CGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPAYPPERLRFILEDAQARL 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 183 VITadegmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemNAEDPLFIL 262
Cdd:cd12115 100 VLT--------------------------------------------------------------------DPDDLAYVI 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 263 YTSGSTGKPKGVV---HTTGGYLVYAAMTFkyvfdyhPGDiYWctADVGWVTG-----HSYLLYGPLACGAMTLMFEGVP 334
Cdd:cd12115 112 YTSGSTGRPKGVAiehRNAAAFLQWAAAAF-------SAE-EL--AGVLASTSicfdlSVFELFGPLATGGKVVLADNVL 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 335 NYPGVNRLGQVidkhqvNILYTAPTAIRALMAEGDKAiegtkrTSLRIMGSVGEPINPEAWEWYYNKiGNGQCpIVDTWW 414
Cdd:cd12115 182 ALPDLPAAAEV------TLINTVPSAAAELLRHDALP------ASVRVVNLAGEPLPRDLVQRLYAR-LQVER-VVNLYG 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 415 QTETGGF-MITPLPGATElKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVItdSWPGQARTLYGD----HDRFEQTYF 489
Cdd:cd12115 248 PSEDTTYsTVAPVPPGAS-GEVSIGRPLANTQAYVLDRALQPVPLGVPGELYI--GGAGVARGYLGRpgltAERFLPDPF 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 490 STFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGE 569
Cdd:cd12115 325 GPGARLYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALRSIPGVREAVVVAIGDAAGERRLVAYIVAEPGA 404
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 1206237686 570 EPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd12115 405 AGLVE---DLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
91-618 |
1.43e-36 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 143.18 E-value: 1.43e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDA 170
Cdd:PRK03640 17 DRTAIEFEE------KKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREE 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 171 VAGRIIDSNAKLVITADEGMRAgraiplkknvddalknpaitsinnvvvfQRTGKTGYWQEgrdlwwhdLIVGVSADC-P 249
Cdd:PRK03640 91 LLWQLDDAEVKCLITDDDFEAK----------------------------LIPGISVKFAE--------LMNGPKEEAeI 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 250 AEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLvYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLL----YGplacga 325
Cdd:PRK03640 135 QEEFDLDEVATIMYTSGTTGKPKGVIQTYGNHW-WSAVGSALNLGLTEDDCWLAAVPIFHISGLSILMrsviYG------ 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 326 MTLMFegVPNYpGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDkaiEGTKRTSLRIMGSVGEPINPEAWEwyynkigng 405
Cdd:PRK03640 208 MRVVL--VEKF-DAEKINKLLQTGGVTIISVVSTMLQRLLERLG---EGTYPSSFRCMLLGGGPAPKPLLE--------- 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 406 QC-----PIVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVDNlGNPQEGASEGNLV-----ITDSWPGQAR 475
Cdd:PRK03640 273 QCkekgiPVYQSYGMTETASQIVTLSPEDALTKLGSAGKPLFPCELKIEKD-GVVVPPFEEGEIVvkgpnVTKGYLNRED 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 476 TLygdHDRFEQTYFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIK 555
Cdd:PRK03640 352 AT---RETFQDGWFKT-------GDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKW 421
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1206237686 556 GQAIYAYITlnHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKI 618
Cdd:PRK03640 422 GQVPVAFVV--KSGEVTEE---ELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQL 479
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
106-615 |
9.95e-36 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 142.09 E-value: 9.95e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 106 KHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVIT 185
Cdd:PRK06710 48 KDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLYTERELEYQLHDSGAKVILC 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 186 ADegmragRAIPLKKNVDDA--LKNPAITSINNVVVFQRTGKTGYWQEGRDlwwhDLIVGVS------------------ 245
Cdd:PRK06710 128 LD------LVFPRVTNVQSAtkIEHVIVTRIADFLPFPKNLLYPFVQKKQS----NLVVKVSesetihlwnsvekevntg 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 246 ADCPAEEMNaeDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDiywctaDVGWVTGHSYLLYGPLACGA 325
Cdd:PRK06710 198 VEVPCDPEN--DLALLQYTGGTTGFPKGVMLTHKNLVSNTLMGVQWLYNCKEGE------EVVLGVLPFFHVYGMTAVMN 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 326 MTLMfEG-----VPNYpGVNRLGQVIDKHQVNILYTAPTAIRALMaeGDKAIEGTKRTSLRIMGSVGEPINPEAWEwYYN 400
Cdd:PRK06710 270 LSIM-QGykmvlIPKF-DMKMVFEAIKKHKVTLFPGAPTIYIALL--NSPLLKEYDISSIRACISGSAPLPVEVQE-KFE 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 401 KIGNGQ----------CPIVDT--WWQTETGGFMITPLPGA----TELKAGSATRPffgvqpalvdnlGNPQEGASEGNL 464
Cdd:PRK06710 345 TVTGGKlvegygltesSPVTHSnfLWEKRVPGSIGVPWPDTeamiMSLETGEALPP------------GEIGEIVVKGPQ 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 465 VITDSWpgqartlygdhDRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAE 544
Cdd:PRK06710 413 IMKGYW-----------NKPEETAAVLQDGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQE 481
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 545 AAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:PRK06710 482 VVTIGVPDPYRGETVKAFVVLKEGTECSEE---ELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVL 549
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
104-620 |
1.29e-35 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 141.83 E-value: 1.29e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 104 QSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLV 183
Cdd:PRK12583 42 QALRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRASELEYALGQSGVRWV 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 184 ITADE-------GMRAGRAIPLKKNVDDALKNPAITSINNVVVFQRTGKTGYwqegrdLWWHDLIV---GVSADCPAE-- 251
Cdd:PRK12583 122 ICADAfktsdyhAMLQELLPGLAEGQPGALACERLPELRGVVSLAPAPPPGF------LAWHELQArgeTVSREALAErq 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 252 -EMNAEDPLFILYTSGSTGKPKGVVHT-----TGGYLVYAAMTF----KYVFdyhPGDIYWC----TADVGWVTGHSYLL 317
Cdd:PRK12583 196 aSLDRDDPINIQYTSGTTGFPKGATLShhnilNNGYFVAESLGLtehdRLCV---PVPLYHCfgmvLANLGCMTVGACLV 272
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 318 Y-----GPLAcgamTLmfegvpnypgvnrlgQVIDKHQVNILYTAPTAIRALMAEGDKAieGTKRTSLR--IMGsvGEPI 390
Cdd:PRK12583 273 YpneafDPLA----TL---------------QAVEEERCTALYGVPTMFIAELDHPQRG--NFDLSSLRtgIMA--GAPC 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 391 NPEAWEwyyNKIGNGQCP-IVDTWWQTETGGfmITPLPGAT---ELKAGSATRPFFGVQPALVDNLGN--PQ----EGAS 460
Cdd:PRK12583 330 PIEVMR---RVMDEMHMAeVQIAYGMTETSP--VSLQTTAAddlERRVETVGRTQPHLEVKVVDPDGAtvPRgeigELCT 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 461 EGNLVITDSWPGQARTLYG-DHDrfeqtyfstfkGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSH 539
Cdd:PRK12583 405 RGYSVMKGYWNNPEATAESiDED-----------GWMHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTH 473
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 540 PKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIA 619
Cdd:PRK12583 474 PAVADVQVFGVPDEKYGEEIVAWVRLHPGHAASEE---ELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQKFRMREIS 550
|
.
gi 1206237686 620 A 620
Cdd:PRK12583 551 I 551
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
82-620 |
8.55e-35 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 138.74 E-value: 8.55e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIwEGDdpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAV--- 158
Cdd:COG1021 31 LRRRAERHPDRIAVV-DGE-----RRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAIpvf 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 159 ------HSVIfggfspDAVAGRiidSNAKLVITADEgmRAG---RAIP--LKKNVDdalknpaitSINNVVVFQRTGktg 227
Cdd:COG1021 105 alpahrRAEI------SHFAEQ---SEAVAYIIPDR--HRGfdyRALAreLQAEVP---------SLRHVLVVGDAG--- 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 228 ywqEGRDLwwhDLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLvYAAMTFKYVFDYHPGDIYWCTADV 307
Cdd:COG1021 162 ---EFTSL---DALLAAPADLSEPRPDPDDVAFFQLSGGTTGLPKLIPRTHDDYL-YSVRASAEICGLDADTVYLAALPA 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 308 GwvtgHSYllygPLAC----GAM----TLMFEGVPNYPGVNRLgqvIDKHQVNILYTAPTAIRALMAEGDKaiEGTKRTS 379
Cdd:COG1021 235 A----HNF----PLSSpgvlGVLyaggTVVLAPDPSPDTAFPL---IERERVTVTALVPPLALLWLDAAER--SRYDLSS 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 380 LRIMGSVGEPINPEAWEWYYNKIGngqCpivdtWWQ-----TEtGGFMITPLPGATELKAGSATRPffgVQPA----LVD 450
Cdd:COG1021 302 LRVLQVGGAKLSPELARRVRPALG---C-----TLQqvfgmAE-GLVNYTRLDDPEEVILTTQGRP---ISPDdevrIVD 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 451 NLGNPQEGASEGNLVitdswpgqAR---TLYG-----DHDrfeQTYFsTFKGMYFSGDGARRDEDGYYWITGRVDDVLNV 522
Cdd:COG1021 370 EDGNPVPPGEVGELL--------TRgpyTIRGyyrapEHN---ARAF-TPDGFYRTGDLVRRTPDGYLVVEGRAKDQINR 437
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 523 SGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNhGEEPTPElytEVRNWVRkEIGpIAT---PDILHWT 599
Cdd:COG1021 438 GGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPR-GEPLTLA---ELRRFLR-ERG-LAAfklPDRLEFV 511
|
570 580
....*....|....*....|.
gi 1206237686 600 DSLPKTRSGKIMRRILRKIAA 620
Cdd:COG1021 512 DALPLTAVGKIDKKALRAALA 532
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
79-631 |
1.29e-34 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 138.37 E-value: 1.29e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 79 ANCLDRHLTERGEQTAIIWEGDDpsqskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAV 158
Cdd:PRK07786 20 VNQLARHALMQPDAPALRFLGNT------TTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAI 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 159 HSVIFGGFSPDAVAGRIIDSNAKLVITadEGMRAgraiPLKKNVDDAlkNPAITSInnVVVFQRTgktgywqEGRDLWWH 238
Cdd:PRK07786 94 AVPVNFRLTPPEIAFLVSDCGAHVVVT--EAALA----PVATAVRDI--VPLLSTV--VVAGGSS-------DDSVLGYE 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 239 DLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVV--HTTggyLVYAAMTFKYVFDYHPGDiywctaDVGWVTGHSYL 316
Cdd:PRK07786 157 DLLAEAGPAHAPVDIPNDSPALIMYTSGTTGRPKGAVltHAN---LTGQAMTCLRTNGADINS------DVGFVGVPLFH 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 317 LYGPLACGAMTLMfeGVPN--YP-GVNRLGQVID---KHQVNILYTAPTAIRALMAEGDKAiegTKRTSLRIMGSVGEPI 390
Cdd:PRK07786 228 IAGIGSMLPGLLL--GAPTviYPlGAFDPGQLLDvleAEKVTGIFLVPAQWQAVCAEQQAR---PRDLALRVLSWGAAPA 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 391 NPEAWEWYYNKIGNGQcpIVDTWWQTEtggfmITP----LPGATEL-KAGSATRPFFGVQPALVDNLGNPQEGASEGNLV 465
Cdd:PRK07786 303 SDTLLRQMAATFPEAQ--ILAAFGQTE-----MSPvtcmLLGEDAIrKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIV 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 466 ItdswpgQARTLYGDHDRFEQTYFSTFKGMYF-SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAE 544
Cdd:PRK07786 376 Y------RAPTLMSGYWNNPEATAEAFAGGWFhSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVE 449
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 545 AAVVGVPHNIKGQAIYAYITLNHGEEPTPelYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK-IAAGDT 623
Cdd:PRK07786 450 VAVIGRADEKWGEVPVAVAAVRNDDAALT--LEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRErYGACVN 527
|
....*...
gi 1206237686 624 SNLGDTST 631
Cdd:PRK07786 528 VERRSASA 535
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
72-617 |
4.05e-34 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 137.44 E-value: 4.05e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 72 DGTLnlsANCLDRHLTERGEQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLA 151
Cdd:PRK05605 31 DTTL---VDLYDNAVARFGDRPALDFFG------ATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYA 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 152 CARIGAV---HSVIFggfSPDAVAGRIIDSNAKLVITAD------EGMRAG------------RAIPLKKNVddALKNP- 209
Cdd:PRK05605 102 VLRLGAVvveHNPLY---TAHELEHPFEDHGARVAIVWDkvaptvERLRRTtpletivsvnmiAAMPLLQRL--ALRLPi 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 210 --------AIT-SINNVVVFQRTGKTGywqEGRDlwwhdlivGVSADCPaeEMNAEDPLFILYTSGSTGKPKGVVHTTGG 280
Cdd:PRK05605 177 palrkaraALTgPAPGTVPWETLVDAA---IGGD--------GSDVSHP--RPTPDDVALILYTSGTTGKPKGAQLTHRN 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 281 YLVYAAMTfkyvfdyhpgdiywctadVGWVTG---------------HSY-----LLYGPLACGAMTLMfegvPNyPGVN 340
Cdd:PRK05605 244 LFANAAQG------------------KAWVPGlgdgpervlaalpmfHAYgltlcLTLAVSIGGELVLL----PA-PDID 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 341 RLGQVIDKHQVNILYTAPTAIRALMAEGDKaiEGTKRTSLRIMGSVGEPINPEawewyynkigngqcpIVDTWwQTETGG 420
Cdd:PRK05605 301 LILDAMKKHPPTWLPGVPPLYEKIAEAAEE--RGVDLSGVRNAFSGAMALPVS---------------TVELW-EKLTGG 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 421 FMI--------TPL----PGATELKAGSATRPFFGVQPALVD--NLGNPQEGASEGNLVItdSWPgqaRTLYGDHDRFEQ 486
Cdd:PRK05605 363 LLVegygltetSPIivgnPMSDDRRPGYVGVPFPDTEVRIVDpeDPDETMPDGEEGELLV--RGP---QVFKGYWNRPEE 437
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 487 TYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLN 566
Cdd:PRK05605 438 TAKSFLDGWFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLE 517
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 567 HGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:PRK05605 518 PGAALDPE---GLRAYCREHLTRYKVPRRFYHVDELPRDQLGKVRRREVRE 565
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
116-616 |
4.95e-34 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 135.26 E-value: 4.95e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 116 NVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDavagrIIDSNAKLVItADEGMRAgrA 195
Cdd:cd05922 2 GVSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRLGLVFVPLNPT-----LKESVLRYLV-ADAGGRI--V 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 196 IPLKKNVD-DALKNPAitsinnvvvfqrTGKTGYWQEGRDLWwhdlivGVSADCPAEEMNAEDPLFILYTSGSTGKPKGV 274
Cdd:cd05922 74 LADAGAADrLRDALPA------------SPDPGTVLDADGIR------AARASAPAHEVSHEDLALLLYTSGSTGSPKLV 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 275 V--HTTggyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGpLACGAMTLMFEGvpnypgvNRLGQ-VID---K 348
Cdd:cd05922 136 RlsHQN---LLANARSIAEYLGITADDRALTVLPLSYDYGLSVLNTH-LLRGATLVLTND-------GVLDDaFWEdlrE 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 349 HQVNILYTAPTaIRALMAEGDKAIEGTkrTSLRIMGSVGEPINPEAWEWYYNKIGNGQcpIVDTWWQTETGGFMiTPLPG 428
Cdd:cd05922 205 HGATGLAGVPS-TYAMLTRLGFDPAKL--PSLRYLTQAGGRLPQETIARLRELLPGAQ--VYVMYGQTEATRRM-TYLPP 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 429 ATEL-KAGSATRPFFGVQPALVDNLGNPQ------EGASEGNLVITDSWpgqartlygdHDRFEQTYFSTFKGMYFSGDG 501
Cdd:cd05922 279 ERILeKPGSIGLAIPGGEFEILDDDGTPTppgepgEIVHRGPNVMKGYW----------NDPPYRRKEGRGGGVLHTGDL 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 502 ARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIkGQAIYAYITLNHGEEPTPelyteVRN 581
Cdd:cd05922 349 ARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPDPL-GEKLALFVTAPDKIDPKD-----VLR 422
|
490 500 510
....*....|....*....|....*....|....*
gi 1206237686 582 WVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd05922 423 SLAERLPPYKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
82-549 |
5.55e-34 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 137.15 E-value: 5.55e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEGDDPSQSkhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:COG1022 17 LRRRAARFPDRVALREKEDGIWQS--LTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVP 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVITADEGMRAgRAIPLKKNvddalknpaITSINNVVVFQRTGKTGywqEGRDLWWHDLI 241
Cdd:COG1022 95 IYPTSSAEEVAYILNDSGAKVLFVEDQEQLD-KLLEVRDE---------LPSLRHIVVLDPRGLRD---DPRLLSLDELL 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 242 -VGVSADCPAE------EMNAEDPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDiywctadvgwvtghS 314
Cdd:COG1022 162 aLGREVADPAElearraAVKPDDLATIIYTSGTTGRPKGVMLTHRN-LLSNARALLERLPLGPGD--------------R 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 315 YLLYGPLA-----CGAMTLMFEGVPNY--PGVNRLGQVIDKHQVNILYTAP-------TAIRALMAEG--------DKAI 372
Cdd:COG1022 227 TLSFLPLAhvferTVSYYALAAGATVAfaESPDTLAEDLREVKPTFMLAVPrvwekvyAGIQAKAEEAgglkrklfRWAL 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 373 EGTKRTS-----------------------------------LRIMGSVGEPINPEAWEWYYNkIGngqCPIVDTWWQTE 417
Cdd:COG1022 307 AVGRRYArarlagkspslllrlkhaladklvfsklrealggrLRFAVSGGAALGPELARFFRA-LG---IPVLEGYGLTE 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 418 TGGFMITPLPGATelKAGSATRPFFGVQPALVDN--------------LGNPQEGASegnlVIT-DSWpgqartlygdhd 482
Cdd:COG1022 383 TSPVITVNRPGDN--RIGTVGPPLPGVEVKIAEDgeilvrgpnvmkgyYKNPEATAE----AFDaDGW------------ 444
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 483 rfeqtyFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGhrlGT----AEIESALVSHPKIAEAAVVG 549
Cdd:COG1022 445 ------LHT-------GDIGELDEDGFLRITGRKKDLIVTSG---GKnvapQPIENALKASPLIEQAVVVG 499
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
108-617 |
6.67e-33 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 131.31 E-value: 6.67e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLvitad 187
Cdd:cd05912 2 YTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDVKL----- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 egmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemnaEDPLFILYTSGS 267
Cdd:cd05912 77 --------------------------------------------------------------------DDIATIMYTSGT 88
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 268 TGKPKGVVHTTGGYLvYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLM--FEgvpnypgVNRLGQV 345
Cdd:cd05912 89 TGKPKGVQQTFGNHW-WSAIGSALNLGLTEDDNWLCALPLFHISGLSILMRSVIYGMTVYLVdkFD-------AEQVLHL 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 346 IDKHQVNILYTAPTAIRALMAEGDkaieGTKRTSLRIMGSVGEPINPEAWEwyynkigngQC-----PIVDTWWQTETGG 420
Cdd:cd05912 161 INSGKVTIISVVPTMLQRLLEILG----EGYPNNLRCILLGGGPAPKPLLE---------QCkekgiPVYQSYGMTETCS 227
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 421 FMITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEgasEGNLV-----ITDSWPGQARTlygDHDRFEQTYFSTfkgm 495
Cdd:cd05912 228 QIVTLSPEDALNKIGSAGKPLFPVELKIEDDGQPPYE---VGEILlkgpnVTKGYLNRPDA---TEESFENGWFKT---- 297
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 496 yfsGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITlnhGEEPTPEl 575
Cdd:cd05912 298 ---GDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVV---SERPISE- 370
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 1206237686 576 yTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:cd05912 371 -EELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
109-622 |
1.14e-32 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 135.86 E-value: 1.14e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADe 188
Cdd:PRK12316 538 DYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYMLEDSGVQLLLSQS- 616
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 189 gmRAGRAIPLKKNVDD-ALKNPAItsinnvvvfqrtgktgyWQEGRdlwwhdlivgvSADCPAEEMNAEDPLFILYTSGS 267
Cdd:PRK12316 617 --HLGRKLPLAAGVQVlDLDRPAA-----------------WLEGY-----------SEENPGTELNPENLAYVIYTSGS 666
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 268 TGKPKGVVHTTGGYLVY-AAMTFKYVFDYHPGDIYWCT--ADVGwvtghSYLLYGPLACGA-MTLMFEGVPNYPgvNRLG 343
Cdd:PRK12316 667 TGKPKGAGNRHRALSNRlCWMQQAYGLGVGDTVLQKTPfsFDVS-----VWEFFWPLMSGArLVVAAPGDHRDP--AKLV 739
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 344 QVIDKHQVNILYTAPTAIRALMAEGDKAiegtKRTSLRIMGSVGEPINPEAWEWYYNKIGngQCPIVDTWWQTETGgfmi 423
Cdd:PRK12316 740 ELINREGVDTLHFVPSMLQAFLQDEDVA----SCTSLRRIVCSGEALPADAQEQVFAKLP--QAGLYNLYGPTEAA---- 809
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 424 TPLPGATELKAGSAT----RPFFGVQPALVDNLGNPQEGASEGNLVITDSwpGQARTLYG----DHDRFEQTYFSTFKGM 495
Cdd:PRK12316 810 IDVTHWTCVEEGGDSvpigRPIANLACYILDANLEPVPVGVLGELYLAGR--GLARGYHGrpglTAERFVPSPFVAGERM 887
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 496 YFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVphniKGQAIYAYITLnhgEEPTPEL 575
Cdd:PRK12316 888 YRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLAV----DGKQLVGYVVL---ESEGGDW 960
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 1206237686 576 YTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGD 622
Cdd:PRK12316 961 REALKAHLAASLPEYMVPAQWLALERLPLTPNGKLDRKALPAPEASV 1007
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
91-615 |
1.27e-32 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 131.68 E-value: 1.27e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWEGDdpsqskHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIfggfSPDA 170
Cdd:cd17655 12 DHTAVVFEDQ------TLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPI----DPDY 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 171 VAGRII----DSNAKLVITADegmragraiPLKKNVDDAlknPAITSINNvvvfqrtgKTGYWQEGRDLwwhdlivgvsa 246
Cdd:cd17655 82 PEERIQyileDSGADILLTQS---------HLQPPIAFI---GLIDLLDE--------DTIYHEESENL----------- 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 247 dcpAEEMNAEDPLFILYTSGSTGKPKGVVHTTGG---YLVYA------------AMTFKYVFDYHPGDIywctadvgwvt 311
Cdd:cd17655 131 ---EPVSKSDDLAYVIYTSGSTGKPKGVMIEHRGvvnLVEWAnkviyqgehlrvALFASISFDASVTEI----------- 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 312 ghsyllYGPLACGAMTLMFEGVPNYPGVnRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEgtkrtSLRIMGSVGEPIN 391
Cdd:cd17655 197 ------FASLLSGNTLYIVRKETVLDGQ-ALTQYIRQNRITIIDLTPAHLKLLDAADDSEGL-----SLKHLIVGGEALS 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 392 PE-AWEWYynKIGNGQCPIVDTWWQTETG-GFMITPLPGATELKAG-SATRPFFGVQPALVDNLGNPQEGASEGNLVItd 468
Cdd:cd17655 265 TElAKKII--ELFGTNPTITNAYGPTETTvDASIYQYEPETDQQVSvPIGKPLGNTRIYILDQYGRPQPVGVAGELYI-- 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 469 SWPGQARTlYGDH-----DRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIA 543
Cdd:cd17655 341 GGEGVARG-YLNRpeltaEKFVDDPFVPGERMYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHPDIK 419
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1206237686 544 EAAVVGVPHNIKGQAIYAYITLNhgEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd17655 420 EAVVIARKDEQGQNYLCAYIVSE--KELPVA---QLREFLARELPDYMIPSYFIKLDEIPLTPNGKVDRKAL 486
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
107-615 |
1.63e-32 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 131.24 E-value: 1.63e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 107 HVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIfggfSPDAVAGRI----IDSNAKL 182
Cdd:cd12114 12 TLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPV----DIDQPAARReailADAGARL 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 183 VITADEgmragraiplkknvDDALKNPAITSINNVVVFQRTgktgywqegrdlwwhdlivgvSADCPAEEMNAEDPLFIL 262
Cdd:cd12114 88 VLTDGP--------------DAQLDVAVFDVLILDLDALAA---------------------PAPPPPVDVAPDDLAYVI 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 263 YTSGSTGKPKGVVHTTGGYL-VYAAMTFKYVFDyhPGDIYWCTADVGW---VtghsYLLYGPLACGAmTLMFEGVPNYPG 338
Cdd:cd12114 133 FTSGSTGTPKGVMISHRAALnTILDINRRFAVG--PDDRVLALSSLSFdlsV----YDIFGALSAGA-TLVLPDEARRRD 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 339 VNRLGQVIDKHQVNILYTAPtAIRALMAEGDKAIEGTKRtSLR-IMGSvGEPINPEawewyynkigngqcpIVDTWWQTE 417
Cdd:cd12114 206 PAHWAELIERHGVTLWNSVP-ALLEMLLDVLEAAQALLP-SLRlVLLS-GDWIPLD---------------LPARLRALA 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 418 TGGFMITpLPGATELKAGSAT----------------RPFFGVQPALVDNLGNPQEGASEGNLVItdSWPGQARTLYGDH 481
Cdd:cd12114 268 PDARLIS-LGGATEASIWSIYhpidevppdwrsipygRPLANQRYRVLDPRGRDCPDWVPGELWI--GGRGVALGYLGDP 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 482 DRFEQTYFSTFKG--MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPhNIKGQAI 559
Cdd:cd12114 345 ELTAARFVTHPDGerLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVARAVVVVLG-DPGGKRL 423
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 1206237686 560 YAYITL-NHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd12114 424 AAFVVPdNDGTPIAPD---ALRAFLAQTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
109-623 |
2.92e-32 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 130.70 E-value: 2.92e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLvITADE 188
Cdd:PRK09088 24 TYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEPRL-LLGDD 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 189 GMRAGRAIPLkknvddalknpaitsinnvvvfqrtgktgywqegrDLwwHDLIVGVSADCPA--EEMNAEDPLFILYTSG 266
Cdd:PRK09088 103 AVAAGRTDVE-----------------------------------DL--AAFIASADALEPAdtPSIPPERVSLILFTSG 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 267 STGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLM---FE------------ 331
Cdd:PRK09088 146 TSGQPKGVMLSERN-LQQTAHNFGVLGRVDAHSSFLCDAPMFHIIGLITSVRPVLAVGGSILVsngFEpkrtlgrlgdpa 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 332 -GVPNYPGVNRLGQVIdKHQVNIlytAPTAIRALMA--EGdkaieGTKRTSLRIMGSVGEPInpeawewyynkigngqcP 408
Cdd:PRK09088 225 lGITHYFCVPQMAQAF-RAQPGF---DAAALRHLTAlfTG-----GAPHAAEDILGWLDDGI-----------------P 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 409 IVDTWWQTETGGFMITPL-PGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVIT--DSWPGQARTLYGDHDRFe 485
Cdd:PRK09088 279 MVDGFGMSEAGTVFGMSVdCDVIRAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLLRgpNLSPGYWRRPQATARAF- 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 486 qtyfsTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITL 565
Cdd:PRK09088 358 -----TGDGWFRTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVP 432
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 566 NHGeepTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR-KIAAGDT 623
Cdd:PRK09088 433 ADG---APLDLERIRSHLSTRLAKYKVPKHLRLVDALPRTASGKLQKARLRdALAAGRK 488
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
263-620 |
5.05e-32 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 127.60 E-value: 5.05e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 263 YTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAmTLMFEGVPNYPG---V 339
Cdd:cd05944 9 HTGGTTGTPKLAQHTHSN-EVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGA-HVVLAGPAGYRNpglF 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 340 NRLGQVIDKHQVNILYTAPTAIRALMAEGDKAiegtKRTSLRIMGSVGEPINPEAWEWYYNKIGngqCPIVDTWWQTE-T 418
Cdd:cd05944 87 DNFWKLVERYRITSLSTVPTVYAALLQVPVNA----DISSLRFAMSGAAPLPVELRARFEDATG---LPVVEGYGLTEaT 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 419 GGFMITPLPGATELKAGSATRPFFGVQPALVDNLGNPQE--GASE-GNLVIT--DSWPGqarTLYGDHDR---FEQTYFS 490
Cdd:cd05944 160 CLVAVNPPDGPKRPGSVGLRLPYARVRIKVLDGVGRLLRdcAPDEvGEICVAgpGVFGG---YLYTEGNKnafVADGWLN 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 491 TfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEE 570
Cdd:cd05944 237 T-------GDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAV 309
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 571 PTPElytEVRNWVRKEIGP-IATPDILHWTDSLPKTRSGKIMRRILRKIAA 620
Cdd:cd05944 310 VEEE---ELLAWARDHVPErAAVPKHIEVLEELPVTAVGKVFKPALRADAI 357
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
87-623 |
5.93e-32 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 130.93 E-value: 5.93e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 87 TERGEQTAIIWEGDDpsqskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIfggf 166
Cdd:PRK06178 44 RERPQRPAIIFYGHV------ITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPV---- 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 167 SPDAVAGRII----DSNAKLVITADegmragRAIPLKKNVDD--ALKNPAITSINNVVVFQRTGKTGYWQEGRDLW---W 237
Cdd:PRK06178 114 SPLFREHELSyelnDAGAEVLLALD------QLAPVVEQVRAetSLRHVIVTSLADVLPAEPTLPLPDSLRAPRLAaagA 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 238 HDL---IVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGgYLVYAAMTFKYVfdYHPGDiywcTADVG------ 308
Cdd:PRK06178 188 IDLlpaLRACTAPVPLPPPALDALAALNYTGGTTGMPKGCEHTQR-DMVYTAAAAYAV--AVVGG----EDSVFlsflpe 260
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 309 -WVTGHSYLLYGPLACGAmTLMF----------EGVPNYpGVNRLGQVIDKHqVNILYTAPTAIRALmaegdkaiegtkr 377
Cdd:PRK06178 261 fWIAGENFGLLFPLFSGA-TLVLlarwdavafmAAVERY-RVTRTVMLVDNA-VELMDHPRFAEYDL------------- 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 378 TSLRIMGSVG--EPINPEawewYYNKigngqcpivdtwWQTETGGFMITPLPGATELK-AGSATRPF------FGVQPAL 448
Cdd:PRK06178 325 SSLRQVRVVSfvKKLNPD----YRQR------------WRALTGSVLAEAAWGMTETHtCDTFTAGFqdddfdLLSQPVF 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 449 VdnlGNPQEGA----------------SEGNLVItdswpgqaRT---LYGDHDRFEQTYFSTFKGMYFSGDGARRDEDGY 509
Cdd:PRK06178 389 V---GLPVPGTefkicdfetgellplgAEGEIVV--------RTpslLKGYWNKPEATAEALRDGWLHTGDIGKIDEQGF 457
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 510 YWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGP 589
Cdd:PRK06178 458 LHYLGRRKEMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGADLTAA---ALQAWCRENMAV 534
|
570 580 590
....*....|....*....|....*....|....
gi 1206237686 590 IATPDIlHWTDSLPKTRSGKIMRRILRKIAAGDT 623
Cdd:PRK06178 535 YKVPEI-RIVDALPMTATGKVRKQDLQALAEELK 567
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
108-615 |
9.68e-32 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 128.35 E-value: 9.68e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITad 187
Cdd:cd17650 13 LTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYMLEDSGAKLLLT-- 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 egmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemNAEDPLFILYTSGS 267
Cdd:cd17650 91 ------------------------------------------------------------------QPEDLAYVIYTSGT 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 268 TGKPKGVVHTTGGYL-VYAAMTFKYVFDYHPGDIYWcTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNYPgvNRLGQVI 346
Cdd:cd17650 105 TGKPKGVMVEHRNVAhAAHAWRREYELDSFPVRLLQ-MASFSFDVFAGDFARSLLNGGTLVICPDEVKLDP--AALYDLI 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 347 DKHQVNILYTAPTAIRALMAEGDKaiEGTKRTSLR--IMGSVGEPINPEAWEwyYNKIGNGQcPIVDTWWQTE----TGG 420
Cdd:cd17650 182 LKSRITLMESTPALIRPVMAYVYR--NGLDLSAMRllIVGSDGCKAQDFKTL--AARFGQGM-RIINSYGVTEatidSTY 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 421 FMIT--PLPGATELKAGsatRPFFGVQPALVDNLGNPQEGASEGNLVITDSwpGQARTLYGDHD----RFEQTYFSTFKG 494
Cdd:cd17650 257 YEEGrdPLGDSANVPIG---RPLPNTAMYVLDERLQPQPVGVAGELYIGGA--GVARGYLNRPEltaeRFVENPFAPGER 331
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 495 MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVgVPHNIKGQA-IYAYITLNHgeepTP 573
Cdd:cd17650 332 MYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVA-VREDKGGEArLCAYVVAAA----TL 406
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 1206237686 574 ELyTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd17650 407 NT-AELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
75-617 |
1.38e-31 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 129.39 E-value: 1.38e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 75 LNLsANCLDRHLTERGEQTAIIWeGDdpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACAR 154
Cdd:PRK07470 7 MNL-AHFLRQAARRFPDRIALVW-GD-----RSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 155 IGAVHSVIFGGFSPDAVAGRIIDSNAKLVITAD---EGMRAGRAiplkknvddalKNPAITSInnVVVFQRTGKTGYwqe 231
Cdd:PRK07470 80 LGAVWVPTNFRQTPDEVAYLAEASGARAMICHAdfpEHAAAVRA-----------ASPDLTHV--VAIGGARAGLDY--- 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 232 grdlwwhDLIVGVSADCPAEEMNAE--DPLFILYTSGSTGKPKGVVHTTGgylvyaAMTFkyVFDYHPGDIYWCT--ADV 307
Cdd:PRK07470 144 -------EALVARHLGARVANAAVDhdDPCWFFFTSGTTGRPKAAVLTHG------QMAF--VITNHLADLMPGTteQDA 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 308 GWVTghsyllyGPLACGAmtlmfegvpnypGVNRLGQV----------------------IDKHQVNILYTAPTaIRALM 365
Cdd:PRK07470 209 SLVV-------APLSHGA------------GIHQLCQVargaatvllpserfdpaevwalVERHRVTNLFTVPT-ILKML 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 366 AEgDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNgqcPIVDTWWQTETGGfMITPLPGA-------TELKAGSAT 438
Cdd:PRK07470 269 VE-HPAVDRYDHSSLRYVIYAGAPMYRADQKRALAKLGK---VLVQYFGLGEVTG-NITVLPPAlhdaedgPDARIGTCG 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 439 RPFFGVQPALVDNLGNPQEGASEGNL-VItdswpGQArTLYGDHDRFEQTYfSTFKGMYF-SGDGARRDEDGYYWITGRV 516
Cdd:PRK07470 344 FERTGMEVQIQDDEGRELPPGETGEIcVI-----GPA-VFAGYYNNPEANA-KAFRDGWFrTGDLGHLDARGFLYITGRA 416
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 517 DDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDIL 596
Cdd:PRK07470 417 SDMYISGGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVDEA---ELLAWLDGKVARYKLPKRF 493
|
570 580
....*....|....*....|.
gi 1206237686 597 HWTDSLPKTRSGKIMRRILRK 617
Cdd:PRK07470 494 FFWDALPKSGYGKITKKMVRE 514
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
531-609 |
1.98e-31 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 116.88 E-value: 1.98e-31
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 531 EIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGK 609
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVELLEE---ELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
108-549 |
2.02e-31 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 127.71 E-value: 2.02e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITAD 187
Cdd:cd05907 6 ITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFVED 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 egmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemnAEDPLFILYTSGS 267
Cdd:cd05907 86 -------------------------------------------------------------------PDDLATIIYTSGT 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 268 TGKPKGVVHTTGGYLvyaaMTFKYVFDYHP---GDIYWCTADVGWVTGHSYLLYGPLACGAM------------------ 326
Cdd:cd05907 99 TGRPKGVMLSHRNIL----SNALALAERLPateGDRHLSFLPLAHVFERRAGLYVPLLAGARiyfassaetllddlsevr 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 327 -TLMFeGVPnypgvnrlgQVIDKHQVNILYTAPTAIRALMAegDKAIEGtkrtSLRIMGSVGEPINPEAWEWyYNKIGng 405
Cdd:cd05907 175 pTVFL-AVP---------RVWEKVYAAIKVKAVPGLKRKLF--DLAVGG----RLRFAASGGAPLPAELLHF-FRALG-- 235
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 406 qCPIVDTWWQTETGGFMITPLPGAteLKAGSATRPFFGVQPALVDN--------------LGNPQEGASEgnlVITDSWp 471
Cdd:cd05907 236 -IPVYEGYGLTETSAVVTLNPPGD--NRIGTVGKPLPGVEVRIADDgeilvrgpnvmlgyYKNPEATAEA---LDADGW- 308
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 472 gqartlygdhdrfeqtyFSTfkgmyfsGDGARRDEDGYYWITGRVDDVL-NVSGHRLGTAEIESALVSHPKIAEAAVVG 549
Cdd:cd05907 309 -----------------LHT-------GDLGEIDEDGFLHITGRKKDLIiTSGGKNISPEPIENALKASPLISQAVVIG 363
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
75-616 |
4.45e-31 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 127.31 E-value: 4.45e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 75 LNLSANcLDRHLTERGEQTAIIWEGDDpsqskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACAR 154
Cdd:PRK06145 2 FNLSAS-IAFHARRTPDRAALVYRDQE------ISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASY 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 155 IGAVHSVIFGGFSPDAVAGRIIDSNAKLVITaDEGMRAGRAIPLKKNVDDALKNPAITsinnvvvfqRTGKTGywqegrd 234
Cdd:PRK06145 75 LGAVFLPINYRLAADEVAYILGDAGAKLLLV-DEEFDAIVALETPKIVIDAAAQADSR---------RLAQGG------- 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 235 lwwhdlivgvsADCPAEEMNAEDPLF-ILYTSGSTGKPKGVVHTTGgylvyaamtfkyvfdyhpgDIYWCTAD----VGW 309
Cdd:PRK06145 138 -----------LEIPPQAAVAPTDLVrLMYTSGTTDRPKGVMHSYG-------------------NLHWKSIDhviaLGL 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 310 VTGHSYLLYGPL-ACGAMTLmfegvpnyPGVNRLGQ-----------------VIDKHQVNILYTAPTAIRALMAEGDK- 370
Cdd:PRK06145 188 TASERLLVVGPLyHVGAFDL--------PGIAVLWVggtlrihrefdpeavlaAIERHRLTCAWMAPVMLSRVLTVPDRd 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 371 ---------AIEGTKRT-SLRImgsvgepinpEAWEWYYNkigNGQcpIVDTWWQTET-GGfmITPLPGATEL-KAGSAT 438
Cdd:PRK06145 260 rfdldslawCIGGGEKTpESRI----------RDFTRVFT---RAR--YIDAYGLTETcSG--DTLMEAGREIeKIGSTG 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 439 RPFFGVQPALVDNLGNPQEGASEGNLVITDswPGQARTLYGDHdrfEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDD 518
Cdd:PRK06145 323 RALAHVEIRIADGAGRWLPPNMKGEICMRG--PKVTKGYWKDP---EKTAEAFYGDWFRSGDVGYLDEEGFLYLTDRKKD 397
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 519 VLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHW 598
Cdd:PRK06145 398 MIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGATLTLE---ALDRHCRQRLASFKVPRQLKV 474
|
570
....*....|....*...
gi 1206237686 599 TDSLPKTRSGKIMRRILR 616
Cdd:PRK06145 475 RDELPRNPSGKVLKRVLR 492
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
88-618 |
1.42e-30 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 126.58 E-value: 1.42e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 88 ERGEQTAIIwegDDPSQskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAI----YMPMVpeaaVAMLACARIGAVHSVIF 163
Cdd:PRK07788 61 RAPDRAALI---DERGT---LTYAELDEQSNALARGLLALGVRAGDGVAVlarnHRGFV----LALYAAGKVGARIILLN 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 164 GGFSPDAVAGRIIDSNAKLVITADEgmRAGRAIPLKKNVDDALknpaitsinnVVVFQRTGKTGYWQEGRDLwwHDLIVG 243
Cdd:PRK07788 131 TGFSGPQLAEVAAREGVKALVYDDE--FTDLLSALPPDLGRLR----------AWGGNPDDDEPSGSTDETL--DDLIAG 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 244 VS-ADCPAEEMNAEdplFILYTSGSTGKPKGVVHTTGGYLVYAAMtfkyVFDYHP---GDIYWCTADVGWVTGHSYLLYG 319
Cdd:PRK07788 197 SStAPLPKPPKPGG---IVILTSGTTGTPKGAPRPEPSPLAPLAG----LLSRVPfraGETTLLPAPMFHATGWAHLTLA 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 320 pLACGAmTLMFEGVPNYPGVNRLgqvIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYY 399
Cdd:PRK07788 270 -MALGS-TVVLRRRFDPEATLED---IAKHKATALVVVPVMLSRILDLGPEVLAKYDTSSLKIIFVSGSALSPELATRAL 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 400 NKIGngqcPIV-DTWWQTETGGFMI-TPlpgaTELKAGSAT--RPFFGVQPALVDNLGNPQEGASEGNLVITDSWPGQAR 475
Cdd:PRK07788 345 EAFG----PVLyNLYGSTEVAFATIaTP----EDLAEAPGTvgRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFPFEGY 416
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 476 TlygdHDRFEQTyfstFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIK 555
Cdd:PRK07788 417 T----DGRDKQI----IDGLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEF 488
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1206237686 556 GQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKI 618
Cdd:PRK07788 489 GQRLRAFVVKAPGAALDED---AIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREM 548
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
90-609 |
2.27e-30 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 125.77 E-value: 2.27e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 90 GEQTAIIWeGDdpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPD 169
Cdd:PRK07798 17 PDRVALVC-GD-----RRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVED 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 170 AVAGRIIDSNAKLVITADEgmRAGRaiplkknVDDALknPAITSINNVVVFQRTGKTGYWQEGRDlwWHDLIVGVSADCP 249
Cdd:PRK07798 91 ELRYLLDDSDAVALVYERE--FAPR-------VAEVL--PRLPKLRTLVVVEDGSGNDLLPGAVD--YEDALAAGSPERD 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 250 AEEMNAEDpLFILYTSGSTGKPKGVVHTTGG-YLV----YAAMTFKYVFDYHpgDIywcTADVGWVTGHSYLLYGPLACG 324
Cdd:PRK07798 158 FGERSPDD-LYLLYTGGTTGMPKGVMWRQEDiFRVllggRDFATGEPIEDEE--EL---AKRAAAGPGMRRFPAPPLMHG 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 325 A-----MTLMFEG--VPNYPGV----NRLGQVIDKHQVNILYTAPTAIRALMAEgdkAIEGTKRT---SLRIMGSVGEPI 390
Cdd:PRK07798 232 AgqwaaFAALFSGqtVVLLPDVrfdaDEVWRTIEREKVNVITIVGDAMARPLLD---ALEARGPYdlsSLFAIASGGALF 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 391 NP---EAW-EWYYNKIgngqcpIVDTWWQTETGgFMITplpGATELKAGSATRPFFGVQP--ALVDNLGNPQEGASEGNL 464
Cdd:PRK07798 309 SPsvkEALlELLPNVV------LTDSIGSSETG-FGGS---GTVAKGAVHTGGPRFTIGPrtVVLDEDGNPVEPGSGEIG 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 465 VItdswpgqART------LYGDHDRFEQTYFsTFKGMYFS--GDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESAL 536
Cdd:PRK07798 379 WI-------ARRghiplgYYKDPEKTAETFP-TIDGVRYAipGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEAL 450
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1206237686 537 VSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGK 609
Cdd:PRK07798 451 KAHPDVADALVVGVPDERWGQEVVAVVQLREGARPDLA---ELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
75-621 |
2.65e-30 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 125.48 E-value: 2.65e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 75 LNLSANCLDRhLTERGEQTAIIwegdDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACAR 154
Cdd:PLN02246 23 LPLHDYCFER-LSEFSDRPCLI----DGATGRVYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASR 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 155 IGAVHSVIFGGFSPDAVAGRIIDSNAKLVITadEGMRAgraiplkknvdDALKNPAITsiNNVVVFQRtgktgywQEGRD 234
Cdd:PLN02246 98 RGAVTTTANPFYTPAEIAKQAKASGAKLIIT--QSCYV-----------DKLKGLAED--DGVTVVTI-------DDPPE 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 235 LWWH--DLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAmtfKYV------FDYHPGDIYWCTAD 306
Cdd:PLN02246 156 GCLHfsELTQADENELPEVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVA---QQVdgenpnLYFHSDDVILCVLP 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 307 VGwvtgHSYLLYGPLACG-----AMTLM--FEgvpnypgVNRLGQVIDKHQVNILYTAPTAIRALmAEGDkAIEGTKRTS 379
Cdd:PLN02246 233 MF----HIYSLNSVLLCGlrvgaAILIMpkFE-------IGALLELIQRHKVTIAPFVPPIVLAI-AKSP-VVEKYDLSS 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 380 LRIMGSVGEPINPEAWEWYYNKIGN---GQcpivdTWWQTETG-------GFMITPLPgateLKAGSATRPFFGVQPALV 449
Cdd:PLN02246 300 IRMVLSGAAPLGKELEDAFRAKLPNavlGQ-----GYGMTEAGpvlamclAFAKEPFP----VKSGSCGTVVRNAELKIV 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 450 DnlgnPQEGASEG-NLvitdswPGQ-------------------ARTLygDHDRFEQTyfstfkgmyfsGDGARRDEDGY 509
Cdd:PLN02246 371 D----PETGASLPrNQ------PGEicirgpqimkgylndpeatANTI--DKDGWLHT-----------GDIGYIDDDDE 427
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 510 YWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGP 589
Cdd:PLN02246 428 LFIVDRLKELIKYKGFQVAPAELEALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGSEITED---EIKQFVAKQVVF 504
|
570 580 590
....*....|....*....|....*....|...
gi 1206237686 590 IATPDILHWTDSLPKTRSGKIMRRILR-KIAAG 621
Cdd:PLN02246 505 YKRIHKVFFVDSIPKAPSGKILRKDLRaKLAAG 537
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
109-618 |
3.67e-29 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 121.51 E-value: 3.67e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLK-KLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITAD 187
Cdd:PRK06839 29 TYKQLHEYVSKVAAYLIyELNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENELIFQLKDSGTTVLFVEK 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 EGMRAGRAIPLKKNVDDALKnpaITSINNVVVFQRTGktgywqegrdlwwhdlivgvsadcpAEEMNAEDPLFILYTSGS 267
Cdd:PRK06839 109 TFQNMALSMQKVSYVQRVIS---ITSLKEIEDRKIDN-------------------------FVEKNESASFIICYTSGT 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 268 TGKPKGVVhttggyLVYAAMTFKYVFDYHPGDIywcTADVGWVTGHSYLLYGPLACGAMTLMFEG----VPNYPGVNRLG 343
Cdd:PRK06839 161 TGKPKGAV------LTQENMFWNALNNTFAIDL---TMHDRSIVLLPLFHIGGIGLFAFPTLFAGgviiVPRKFEPTKAL 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 344 QVIDKHQVNILYTAPTAIRALMAEGDKaiEGTKRTSLRimgsvgepinpeaweWYYNkiGNGQCPIV------------- 410
Cdd:PRK06839 232 SMIEKHKVTVVMGVPTIHQALINCSKF--ETTNLQSVR---------------WFYN--GGAPCPEElmrefidrgflfg 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 411 DTWWQTETGG--FMITPLPGATelKAGSATRPFFGVQPALVDNLGN--PQEGASE----GNLVITDSWpgqartlygdhD 482
Cdd:PRK06839 293 QGFGMTETSPtvFMLSEEDARR--KVGSIGKPVLFCDYELIDENKNkvEVGEVGEllirGPNVMKEYW-----------N 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 483 RFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAY 562
Cdd:PRK06839 360 RPDATEETIQDGWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAF 439
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 563 ITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKI 618
Cdd:PRK06839 440 IVKKSSSVLIEK---DVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQLVNQ 492
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
109-617 |
6.72e-29 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 121.39 E-value: 6.72e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVI---- 184
Cdd:PRK06087 51 TYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWREAELVWVLNKCQAKMFFaptl 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 185 ---TADEGMragrAIPLKKNVD--------DALKnPAITSINNVVVFQRTGKtgywqegrdlwwhdlivgVSADCPAeem 253
Cdd:PRK06087 131 fkqTRPVDL----ILPLQNQLPqlqqivgvDKLA-PATSSLSLSQIIADYEP------------------LTTAITT--- 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 254 NAEDPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEgv 333
Cdd:PRK06087 185 HGDELAAVLFTSGTEGLPKGVMLTHNN-ILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLD-- 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 334 pnypgvnrlgqvidkhqvniLYTAPTAIRALMAEG------------D--KAIE--GTKRTSLRIMGSVGEPInP----- 392
Cdd:PRK06087 262 --------------------IFTPDACLALLEQQRctcmlgatpfiyDllNLLEkqPADLSALRFFLCGGTTI-Pkkvar 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 393 EAWEWyynkiGNGQCPIvdtWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVDN------LGNPQEGASEGNLVI 466
Cdd:PRK06087 321 ECQQR-----GIKLLSV---YGSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDEarktlpPGCEGEEASRGPNVF 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 467 T---DSWPGQARTLygDHDrfeqtyfstfkGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIA 543
Cdd:PRK06087 393 MgylDEPELTARAL--DEE-----------GWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIH 459
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1206237686 544 EAAVVGVPHNIKGQAIYAYITLNhGEEPTPELyTEVRNWV-RKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:PRK06087 460 DACVVAMPDERLGERSCAYVVLK-APHHSLTL-EEVVAFFsRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRK 532
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
104-615 |
1.02e-28 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 119.89 E-value: 1.02e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 104 QSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLV 183
Cdd:cd17656 10 ENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSGVRVV 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 184 ITADEgmragraiplkknvddalkNPAITSINNVVVFqrtgktgywqegrdLWWhDLIVGVSADCPAEEMNAEDPLFILY 263
Cdd:cd17656 90 LTQRH-------------------LKSKLSFNKSTIL--------------LED-PSISQEDTSNIDYINNSDDLLYIIY 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 264 TSGSTGKPKGV-------------------VHTTGGYLVYAAMTFkyvfdyhpgdiywctaDVGWVTGHSYLLYGplacG 324
Cdd:cd17656 136 TSGTTGKPKGVqlehknmvnllhferektnINFSDKVLQFATCSF----------------DVCYQEIFSTLLSG----G 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 325 AMTLMFEGVPNypGVNRLGQVIDKHQVNILYTaPTAIRALMAEGDKAIEGTKrTSLRIMGSVGEP--INPEAWEWY---- 398
Cdd:cd17656 196 TLYIIREETKR--DVEQLFDLVKRHNIEVVFL-PVAFLKFIFSEREFINRFP-TCVKHIITAGEQlvITNEFKEMLhehn 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 399 ---YNKIGNGQCPIVDTwwqtetggFMITPLPGATELKAgsATRPFFGVQPALVDNLGNPQEGASEGNLVItdSWPGQAR 475
Cdd:cd17656 272 vhlHNHYGPSETHVVTT--------YTINPEAEIPELPP--IGKPISNTWIYILDQEQQLQPQGIVGELYI--SGASVAR 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 476 TLYGD----HDRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVP 551
Cdd:cd17656 340 GYLNRqeltAEKFFPDPFDPNERMYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKA 419
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1206237686 552 HNIKGQAIYAYITlnhgeePTPELYT-EVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd17656 420 DDKGEKYLCAYFV------MEQELNIsQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
108-621 |
1.42e-28 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 122.76 E-value: 1.42e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITAD 187
Cdd:PRK12316 4577 LTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPLDPEYPRERLAYMMEDSGAALLLTQS 4656
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 egmRAGRAIPLKKNVDdalknpaitsinnVVVFQRTGKtgyWQegrdlwwhdlivGVSADCPAEEMNAEDPLFILYTSGS 267
Cdd:PRK12316 4657 ---HLLQRLPIPDGLA-------------SLALDRDED---WE------------GFPAHDPAVRLHPDNLAYVIYTSGS 4705
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 268 TGKPKGVVHTTGGYLVYAAMTfkyvfdyhpGDIYWCTADVGWVTGHSYL-------LYGPLACGAMTLMFEgvPNYPGVN 340
Cdd:PRK12316 4706 TGRPKGVAVSHGSLVNHLHAT---------GERYELTPDDRVLQFMSFSfdgshegLYHPLINGASVVIRD--DSLWDPE 4774
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 341 RLGQVIDKHQVNILYTAPTAIRALmAEGDKaiEGTKRTSLRIMGSVGEPINPEAW-EWY--------YNKIGNGQCPIVD 411
Cdd:PRK12316 4775 RLYAEIHEHRVTVLVFPPVYLQQL-AEHAE--RDGEPPSLRVYCFGGEAVAQASYdLAWralkpvylFNGYGPTETTVTV 4851
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 412 TWWQTETGgfmitPLPGATELKAGsatRPFFGVQPALVDNLGNPQEGASEGNLVITDSwpGQARtlyGDH-------DRF 484
Cdd:PRK12316 4852 LLWKARDG-----DACGAAYMPIG---TPLGNRSGYVLDGQLNPLPVGVAGELYLGGE--GVAR---GYLerpaltaERF 4918
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 485 EQTYFSTFKG-MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIkGQAIYAYI 563
Cdd:PRK12316 4919 VPDPFGAPGGrLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAV-GKQLVGYV 4997
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1206237686 564 TLNHGE-EPTPELYTEVRNWVRKEIGPiATPDIL---HWT--DSLPKTRSGKIMRRILRKIAAG 621
Cdd:PRK12316 4998 VPQDPAlADADEAQAELRDELKAALRE-RLPEYMvpaHLVflARMPLTPNGKLDRKALPQPDAS 5060
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
237-617 |
1.62e-28 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 120.17 E-value: 1.62e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 237 WHDLIVGVS-ADCPAEEMNAEDPLFILYTSGSTGKPKGVVhTTGGYLVYAAMTFKYVFDYHPGDIYWCTAD--------V 307
Cdd:PRK07867 132 WADELAAHRdAEPPFRVADPDDLFMLIFTSGTSGDPKAVR-CTHRKVASAGVMLAQRFGLGPDDVCYVSMPlfhsnavmA 210
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 308 GWVTGhsyllygpLACGAMTLM---FEGVPNYPGVNRLG----QVIDKHQVNILYTAPtairalmaEGDKAiegtkRTSL 380
Cdd:PRK07867 211 GWAVA--------LAAGASIALrrkFSASGFLPDVRRYGatyaNYVGKPLSYVLATPE--------RPDDA-----DNPL 269
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 381 RIM-GSVGEPINPEAWEWYYNkigngqCPIVDTWWQTEtGGFMITPLPGATElkaGSATRPFFGVQ-----------PAL 448
Cdd:PRK07867 270 RIVyGNEGAPGDIARFARRFG------CVVVDGFGSTE-GGVAITRTPDTPP---GALGPLPPGVAivdpdtgtecpPAE 339
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 449 VDNLGNPQEGASEGNLVITDSwPGQARTLYGDHDRFEQtyfSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLG 528
Cdd:PRK07867 340 DADGRLLNADEAIGELVNTAG-PGGFEGYYNDPEADAE---RMRGGVYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLG 415
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 529 TAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNwVRKEIGPIATPDILHWTDSLPKTRSG 608
Cdd:PRK07867 416 TAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPGAKFDPDAFAEFLA-AQPDLGPKQWPSYVRVCAELPRTATF 494
|
....*....
gi 1206237686 609 KIMRRILRK 617
Cdd:PRK07867 495 KVLKRQLSA 503
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
107-615 |
1.76e-28 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 118.51 E-value: 1.76e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 107 HVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITa 186
Cdd:cd17652 12 TLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYMLADARPALLLT- 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 187 degmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemNAEDPLFILYTSG 266
Cdd:cd17652 91 -------------------------------------------------------------------TPDNLAYVIYTSG 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 267 STGKPKGVV--HTTGGYLVYAAMTFkyvFDYHPGDIYWCTADVGWVTGHSYLLyGPLACGAMTLMFEGVPNYPGvNRLGQ 344
Cdd:cd17652 104 STGRPKGVVvtHRGLANLAAAQIAA---FDVGPGSRVLQFASPSFDASVWELL-MALLAGATLVLAPAEELLPG-EPLAD 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 345 VIDKHQVNILYTAPTAIRALMAEGDKAiegtkrtsLRIMGSVGEPINPE-AWEW-----YYNKIGNGQCPIVDTWWQTET 418
Cdd:cd17652 179 LLREHRITHVTLPPAALAALPPDDLPD--------LRTLVVAGEACPAElVDRWapgrrMINAYGPTETTVCATMAGPLP 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 419 GGFMIT---PLPGATELKAGSATRPF-FGVQpalvdnlgnpqegaseGNLVItdSWPGQARtlyGDHDRFEQTY------ 488
Cdd:cd17652 251 GGGVPPigrPVPGTRVYVLDARLRPVpPGVP----------------GELYI--AGAGLAR---GYLNRPGLTAerfvad 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 489 -FSTFKG-MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLN 566
Cdd:cd17652 310 pFGAPGSrMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDDRPGDKRLVAYVVPA 389
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 1206237686 567 HGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd17652 390 PGAAPTAA---ELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
109-616 |
1.95e-28 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 119.86 E-value: 1.95e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAvHSVIFG-GFSPDAVAGRIIDSNAKLVITAD 187
Cdd:PRK13382 70 TWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGA-DILLLNtSFAGPALAEVVTREGVDTVIYDE 148
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 EgmragrAIPLkknVDDALKN-PAITSINNvvvfqrtgktgyWQEGRdlwwHDLIVGVSADCPAEEMNAEDPL---FILY 263
Cdd:PRK13382 149 E------FSAT---VDRALADcPQATRIVA------------WTDED----HDLTVEVLIAAHAGQRPEPTGRkgrVILL 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 264 TSGSTGKPKGVVHTTGGylvyAAMTFKYVFDYHPgdiywctadvgWVTGHSYLLYGPL--ACGAMTLMFEGVPNYPGVNR 341
Cdd:PRK13382 204 TSGTTGTPKGARRSGPG----GIGTLKAILDRTP-----------WRAEEPTVIVAPMfhAWGFSQLVLAASLACTIVTR 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 342 LG-------QVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNgqcPIVDTWW 414
Cdd:PRK13382 269 RRfdpeatlDLIDRHRATGLAVVPVMFDRIMDLPAEVRNRYSGRSLRFAAASGSRMRPDVVIAFMDQFGD---VIYNNYN 345
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 415 QTETGgfMITPlpgAT--ELKAG--SATRPFFGVQPALVDNLGNPQEGASEGNLVITDSWPGQARTLYGDHDrfeqtyfs 490
Cdd:PRK13382 346 ATEAG--MIAT---ATpaDLRAApdTAGRPAEGTEIRILDQDFREVPTGEVGTIFVRNDTQFDGYTSGSTKD-------- 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 491 TFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEE 570
Cdd:PRK13382 413 FHDGFMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKPGAS 492
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 1206237686 571 PTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK13382 493 ATPE---TLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQ 535
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
256-616 |
2.86e-28 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 116.22 E-value: 2.86e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 256 EDPLFILYTSGSTGKPKGVVHT-----TGGYLVYAAMTFKyvfdyhPGDI-------YWCTADVGwvtghsyllyGPLAC 323
Cdd:cd05917 2 DDVINIQFTSGTTGSPKGATLThhnivNNGYFIGERLGLT------EQDRlcipvplFHCFGSVL----------GVLAC 65
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 324 ---GAmTLMFEGvPNYPGVNRLgQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKrtSLR--IMGsvGEPINPEAWEWY 398
Cdd:cd05917 66 lthGA-TMVFPS-PSFDPLAVL-EAIEKEKCTALHGVPTMFIAELEHPDFDKFDLS--SLRtgIMA--GAPCPPELMKRV 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 399 YNKIGNGQCPIVdtWWQTETGGFMITPLPGAT-ELKAGSATRPFFGVQPALVD-------NLGNPQEGASEGNLVITDSW 470
Cdd:cd05917 139 IEVMNMKDVTIA--YGMTETSPVSTQTRTDDSiEKRVNTVGRIMPHTEAKIVDpeggivpPVGVPGELCIRGYSVMKGYW 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 471 pgqartlyGDHDRFEQTYfsTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGV 550
Cdd:cd05917 217 --------NDPEKTAEAI--DGDGWLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGV 286
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 551 PHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd05917 287 PDERYGEEVCAWIRLKEGAELTEE---DIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
257-612 |
6.61e-28 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 115.06 E-value: 6.61e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 257 DPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNy 336
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGN-LIAANLQLIHAMGLTEADVYLNMLPLFHIAGLNLALATFHAGGANVVMEKFDPA- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 337 pgvnRLGQVIDKHQVNILYTAPTAIRALMAEGDKAieGTKRTSLRIMGSVGEPINPEAWEwyynKIGNGqcpivdTWW-- 414
Cdd:cd17637 79 ----EALELIEEEKVTLMGSFPPILSNLLDAAEKS--GVDLSSLRHVLGLDAPETIQRFE----ETTGA------TFWsl 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 415 --QTETGGFmITPLPgATElKAGSATRPFFGVQPALVDNLGNPQE-GAS-----EGNLVITDSWPGQARTLYgdhdrfeq 486
Cdd:cd17637 143 ygQTETSGL-VTLSP-YRE-RPGSAGRPGPLVRVRIVDDNDRPVPaGETgeivvRGPLVFQGYWNLPELTAY-------- 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 487 tyfsTFK-GMYFSGDGARRDEDGYYWITGRV--DDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYI 563
Cdd:cd17637 212 ----TFRnGWHHTGDLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVC 287
|
330 340 350 360
....*....|....*....|....*....|....*....|....*....
gi 1206237686 564 TLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMR 612
Cdd:cd17637 288 VLKPGATLTAD---ELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
83-616 |
6.84e-28 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 117.87 E-value: 6.84e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 83 DRHLTERGEQTAIIWEGDDPSqskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVI 162
Cdd:PRK13391 4 GIHAQTTPDKPAVIMASTGEV----VTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 163 FGGFSPDAVAGRIIDSNAKLVITADEGMRAGRAIPlkKNVddalknPAITSinnVVVFQRTGKTGYWQEGRDlwwhdliv 242
Cdd:PRK13391 80 NSHLTPAEAAYIVDDSGARALITSAAKLDVARALL--KQC------PGVRH---RLVLDGDGELEGFVGYAE-------- 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 243 gVSADCPAEEMNAE---DPLfiLYTSGSTGKPKGV--------VHTTGGYLvyaaMTFKYVFDYHPGDIYWCTADVGwvt 311
Cdd:PRK13391 141 -AVAGLPATPIADEslgTDM--LYSSGTTGRPKGIkrplpeqpPDTPLPLT----AFLQRLWGFRSDMVYLSPAPLY--- 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 312 gHSyllyGPLAcgamtlmFEGVpnypgVNRLG---------------QVIDKHQVNILYTAPTA-IRALmaegdKAIEGT 375
Cdd:PRK13391 211 -HS----APQR-------AVML-----VIRLGgtvivmehfdaeqylALIEEYGVTHTQLVPTMfSRML-----KLPEEV 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 376 KRT----SLRIMGSVGEPINPEAWE----WYynkigngqCPIVDTWW-QTETGGFMITPLPGATElKAGSATRPFFGVqP 446
Cdd:PRK13391 269 RDKydlsSLEVAIHAAAPCPPQVKEqmidWW--------GPIIHEYYaATEGLGFTACDSEEWLA-HPGTVGRAMFGD-L 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 447 ALVDNLGNPQEgasegnlvitdswPGQARTLYGDHDR-FEqtYF----------STFKGMYFSGDGARRDEDGYYWITGR 515
Cdd:PRK13391 339 HILDDDGAELP-------------PGEPGTIWFEGGRpFE--YLndpaktaearHPDGTWSTVGDIGYVDEDGYLYLTDR 403
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 516 VDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDI 595
Cdd:PRK13391 404 AAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDGVDPGPALAAELIAFCRQRLSRQKCPRS 483
|
570 580
....*....|....*....|.
gi 1206237686 596 LHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK13391 484 IDFEDELPRLPTGKLYKRLLR 504
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
257-619 |
2.45e-27 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 113.19 E-value: 2.45e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 257 DPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYV-FDyhPGDIYWCTADVGWVTGHSYLLYGpLACGAMTLMFEgvPN 335
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLgFG--GGDSWLLSLPLYHVGGLAILVRS-LLAGAELVLLE--RN 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 336 YPGVNRLGQVIDKHqvniLYTAPTAIRALMAEGdkaIEGTKRTSLRIMGSVGEPINPEAWEwyynKIGNGQCPIVDTWWQ 415
Cdd:cd17630 76 QALAEDLAPPGVTH----VSLVPTQLQRLLDSG---QGPAALKSLRAVLLGGAPIPPELLE----RAADRGIPLYTTYGM 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 416 TETGGfMITPLPgATELKAGSATRPFFGVQPALVDNlgnpqegaseGNLvitdsWPGQARTLYGDHDRFEQTYFSTfKGM 495
Cdd:cd17630 145 TETAS-QVATKR-PDGFGRGGVGVLLPGRELRIVED----------GEI-----WVGGASLAMGYLRGQLVPEFNE-DGW 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 496 YFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNhgEEPTPEl 575
Cdd:cd17630 207 FTTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGR--GPADPA- 283
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 1206237686 576 ytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIA 619
Cdd:cd17630 284 --ELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
82-617 |
7.65e-27 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 114.32 E-value: 7.65e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEgddpsqSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:cd12118 10 LERAAAVYPDRTSIVYG------DRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNA 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVITADEgmragraiplkKNVDDALKnpaitsinnvvvfqrTGKTGY-WQegrdlwwhdl 240
Cdd:cd12118 84 LNTRLDAEEIAFILRHSEAKVLFVDRE-----------FEYEDLLA---------------EGDPDFeWI---------- 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 241 ivgvsadCPAEEMnaeDPLFILYTSGSTGKPKGVVHT-TGGYLvyAAMTFKYVFDYHPGDIYWCTADV----GWVtghsy 315
Cdd:cd12118 128 -------PPADEW---DPIALNYTSGTTGRPKGVVYHhRGAYL--NALANILEWEMKQHPVYLWTLPMfhcnGWC----- 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 316 LLYGPLACGAMTLMFEGVpNYPGVNRLgqvIDKHQVNILYTAPTAIRALMaegDKAIEGTKRTSLRIMGSVGEPINPEAw 395
Cdd:cd12118 191 FPWTVAAVGGTNVCLRKV-DAKAIYDL---IEKHKVTHFCGAPTVLNMLA---NAPPSDARPLPHRVHVMTAGAPPPAA- 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 396 ewYYNKIGNGQCPIVDTWWQTETGGFMIT--------PLPgaTELKAGSATR---PFFGVQPALVDNLGNPQ-------- 456
Cdd:cd12118 263 --VLAKMEELGFDVTHVYGLTETYGPATVcawkpewdELP--TEERARLKARqgvRYVGLEEVDVLDPETMKpvprdgkt 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 457 --EGASEGNLVitdswpgqARTLYGDHdrfEQTYfSTFKGMYF-SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIE 533
Cdd:cd12118 339 igEIVFRGNIV--------MKGYLKNP---EATA-EAFRGGWFhSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVE 406
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 534 SALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDsLPKTRSGKIMRR 613
Cdd:cd12118 407 GVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKVTEE---EIIAFCREHLAGFMVPKTVVFGE-LPKTSTGKIQKF 482
|
....
gi 1206237686 614 ILRK 617
Cdd:cd12118 483 VLRD 486
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
79-619 |
1.08e-26 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 114.46 E-value: 1.08e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 79 ANCLDRHLTERGEQTAIIwEGDDPsqskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAV 158
Cdd:PRK06164 13 ASLLDAHARARPDAVALI-DEDRP-----LSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGAT 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 159 HSVIFGGFSPDAVAGRIIDSNAKLVITADegmrAGRAIPLKKNVDDALKNpAITSINNVVVFQRTGKT--GYWQEGrdlw 236
Cdd:PRK06164 87 VIAVNTRYRSHEVAHILGRGRARWLVVWP----GFKGIDFAAILAAVPPD-ALPPLRAIAVVDDAADAtpAPAPGA---- 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 237 WHDLIVGVSADCPA---EEMNAEDPLFILYT-SGSTGKPKGVVHTTGGYLVYAAMTFKyVFDYHPGDIYWCTADVGWVTG 312
Cdd:PRK06164 158 RVQLFALPDPAPPAaagERAADPDAGALLFTtSGTTSGPKLVLHRQATLLRHARAIAR-AYGYDPGAVLLAALPFCGVFG 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 313 HSYLLyGPLACGAmTLMFEGVPNYPgvnRLGQVIDKHQVNILYTAPTAIRALMAEGDkaiEGTKRTSLRIMGSVG-EPIN 391
Cdd:PRK06164 237 FSTLL-GALAGGA-PLVCEPVFDAA---RTARALRRHRVTHTFGNDEMLRRILDTAG---ERADFPSARLFGFASfAPAL 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 392 PEAWEWyynkigngqcpivdtwwqTETGGFMITPLPGATELKA----GSATRPF--------FGVQPALVDNLGNPQEGA 459
Cdd:PRK06164 309 GELAAL------------------ARARGVPLTGLYGSSEVQAlvalQPATDPVsvriegggRPASPEARVRARDPQDGA 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 460 -----SEGNLVItdSWPGQARTLYGDHDRFEQTYfsTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIES 534
Cdd:PRK06164 371 llpdgESGEIEI--RAPSLMRGYLDNPDATARAL--TDDGYFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEH 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 535 ALVSHPKIAEAAVVGVPHniKGQAI-YAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSG---KI 610
Cdd:PRK06164 447 ALEALPGVAAAQVVGATR--DGKTVpVAFVIPTDGASPDEA---GLMAACREALAGFKVPARVQVVEAFPVTESAngaKI 521
|
....*....
gi 1206237686 611 MRRILRKIA 619
Cdd:PRK06164 522 QKHRLREMA 530
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
82-620 |
1.10e-26 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 116.80 E-value: 1.10e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:PRK12467 3101 IEAQVARTPEAPALVFGD------QQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVP 3174
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVITadegmragraiplKKNVDDALknPAITSINNVVVfqrtgktgywqEGRDLWwhdli 241
Cdd:PRK12467 3175 LDPEYPRERLAYMIEDSGVKLLLT-------------QAHLLEQL--PAPAGDTALTL-----------DRLDLN----- 3223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 242 vGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVY-AAMTFKYVFDYHPGDIYWCTADVgwvTGHSYLLYGP 320
Cdd:PRK12467 3224 -GYSENNPSTRVMGENLAYVIYTSGSTGKPKGVGVRHGALANHlCWIAEAYELDANDRVLLFMSFSF---DGAQERFLWT 3299
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 321 LACGAMTLMFEGVPNYPgvNRLGQVIDKHQVNILYTAPTAIRALMAEGDkaieGTKRTSLRIMGSVGEPINPEAWEWY-- 398
Cdd:PRK12467 3300 LICGGCLVVRDNDLWDP--EELWQAIHAHRISIACFPPAYLQQFAEDAG----GADCASLDIYVFGGEAVPPAAFEQVkr 3373
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 399 -------YNKIGNGQCPIVDTWWQTETGGfmitpLPGATELKAGsatRPFFGVQPALVDNLGNPQEGASEGNLVITDSwp 471
Cdd:PRK12467 3374 klkprglTNGYGPTEAVVTVTLWKCGGDA-----VCEAPYAPIG---RPVAGRSIYVLDGQLNPVPVGVAGELYIGGV-- 3443
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 472 GQARtlyGDH-------DRFEQTYFSTFKG-MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIA 543
Cdd:PRK12467 3444 GLAR---GYHqrpsltaERFVADPFSGSGGrLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVR 3520
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1206237686 544 EAAVVGVPhNIKGQAIYAYITLNhgeEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAA 620
Cdd:PRK12467 3521 EAVVLARD-GAGGKQLVAYVVPA---DPQGDWRETLRDHLAASLPDYMVPAQLLVLAAMPLGPNGKVDRKALPDPDA 3593
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
103-617 |
1.46e-26 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 116.41 E-value: 1.46e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 103 SQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKL 182
Cdd:PRK12467 533 FGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQDRLAYMLDDSGVRL 612
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 183 VITADEGMRagraiplkknvddalKNPAITSINNVVVFQRTgktgywqegrdlwwhDLIVGVSADCPAEEMNAEDPLFIL 262
Cdd:PRK12467 613 LLTQSHLLA---------------QLPVPAGLRSLCLDEPA---------------DLLCGYSGHNPEVALDPDNLAYVI 662
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 263 YTSGSTGKPKGVVHTTGGYLVYAAMTFKYvfdyhpgdiYWCTADVGWVTGHSY-------LLYGPLACGAMTLMFEgvpn 335
Cdd:PRK12467 663 YTSGSTGQPKGVAISHGALANYVCVIAER---------LQLAADDSMLMVSTFafdlgvtELFGALASGATLHLLP---- 729
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 336 yPGVNR----LGQVIDKHQVNILYTAPTAIRALMAEGDKAiEGTKRTSLRIMGSVGEPINPEAWEwyynKIGNGqCPIVD 411
Cdd:PRK12467 730 -PDCARdaeaFAALMADQGVTVLKIVPSHLQALLQASRVA-LPRPQRALVCGGEALQVDLLARVR----ALGPG-ARLIN 802
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 412 TWWQTETG-GFMITPLPGATELKAGSAT-RPFFGVQPALVDNLGNPQEGASEGNLVITDSwpGQARTLYG----DHDRFE 485
Cdd:PRK12467 803 HYGPTETTvGVSTYELSDEERDFGNVPIgQPLANLGLYILDHYLNPVPVGVVGELYIGGA--GLARGYHRrpalTAERFV 880
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 486 QTYFSTFKG-MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPhNIKGQAIYAYIT 564
Cdd:PRK12467 881 PDPFGADGGrLYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLAQP-GDAGLQLVAYLV 959
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 1206237686 565 LNHGEEPT--PELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:PRK12467 960 PAAVADGAehQATRDELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPK 1014
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
106-616 |
1.14e-25 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 111.25 E-value: 1.14e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 106 KHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPdAVAGRII-DSNAKLVI 184
Cdd:PRK13390 23 EQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTA-PEADYIVgDSGARVLV 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 185 T--ADEGM--RAGRAIPLKknvddalknpaitsinnvVVFQrtgktgywqeGRDLWWHDLIVGVSADCPAEemnAEDPL- 259
Cdd:PRK13390 102 AsaALDGLaaKVGADLPLR------------------LSFG----------GEIDGFGSFEAALAGAGPRL---TEQPCg 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 260 -FILYTSGSTGKPKGV--------VHTTGGYLVYAAMTFkyvFDYHPGDIYWCTADVGwvtgHSyllyGPLACGAMTLMF 330
Cdd:PRK13390 151 aVMLYSSGTTGFPKGIqpdlpgrdVDAPGDPIVAIARAF---YDISESDIYYSSAPIY----HA----APLRWCSMVHAL 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 331 EGV----PNYPGVNRLGQvIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEP------------INPEA 394
Cdd:PRK13390 220 GGTvvlaKRFDAQATLGH-VERYRITVTQMVPTMFVRLLKLDADVRTRYDVSSLRAVIHAAAPcpvdvkhamidwLGPIV 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 395 WEWYYNKIGNGQCPIvdtwwqtETGGFMITPlpgatelkaGSATRPFFGVQpALVDNLGNpqegasegnlvitDSWPGQA 474
Cdd:PRK13390 299 YEYYSSTEAHGMTFI-------DSPDWLAHP---------GSVGRSVLGDL-HICDDDGN-------------ELPAGRI 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 475 RTLYGDHDRF--------EQTYFSTFKGMYF---SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIA 543
Cdd:PRK13390 349 GTVYFERDRLpfrylndpEKTAAAQHPAHPFwttVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVH 428
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1206237686 544 EAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK13390 429 DVAVIGVPDPEMGEQVKAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
91-619 |
2.48e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 109.87 E-value: 2.48e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWEgddpsqSKHVTYKQLHHNVCQFANVLKKLGvKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDA 170
Cdd:PRK07638 16 NKIAIKEN------DRVLTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDE 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 171 VAGRIIDSNAKLVITadegmragraiplkknvDDALKNPAITSINNVVVfqrtgktgyWQEgrdlwWHDLIvGVSADCPA 250
Cdd:PRK07638 89 LKERLAISNADMIVT-----------------ERYKLNDLPDEEGRVIE---------IDE-----WKRMI-EKYLPTYA 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 251 EEMNAE-DPLFILYTSGSTGKPKGVVHTTGGYLvyaamtfkYVFDYHPGDIYWCTADVGWVTG---HSYLLYGPLAC--- 323
Cdd:PRK07638 137 PIENVQnAPFYMGFTSGSTGKPKAFLRAQQSWL--------HSFDCNVHDFHMKREDSVLIAGtlvHSLFLYGAISTlyv 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 324 -GAMTLMFEGVPNypgvnrlgQVIDK---HQVNILYTAPTAIRALMAEgdkaiEGTKRTSLRIMGSVGEpinpeaWEwyy 399
Cdd:PRK07638 209 gQTVHLMRKFIPN--------QVLDKletENISVMYTVPTMLESLYKE-----NRVIENKMKIISSGAK------WE--- 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 400 nKIGNGQcpIVDTW--------WQTETGGFMITPLPGATELKAGSATRPFFGVQPALVDNLGnpqegasegnlviTDSWP 471
Cdd:PRK07638 267 -AEAKEK--IKNIFpyaklyefYGASELSFVTALVDEESERRPNSVGRPFHNVQVRICNEAG-------------EEVQK 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 472 GQARTLYGDHDRFEQTYFS--------TFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIA 543
Cdd:PRK07638 331 GEIGTVYVKSPQFFMGYIIggvlarelNADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVD 410
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 544 EAAVVGVPHNIKGQAIYAYItlnHGEEPTPELytevRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIA 619
Cdd:PRK07638 411 EIVVIGVPDSYWGEKPVAII---KGSATKQQL----KSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSWI 479
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
106-622 |
5.45e-25 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 111.59 E-value: 5.45e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 106 KHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVIT 185
Cdd:PRK12316 3081 QRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLLLS 3160
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 186 ADEgmragraiplkknvddaLKNPAITSINNVVVfqRTGKTGYWQEGrdlwwhdlivgvsadcPAEEMNAEDPLFILYTS 265
Cdd:PRK12316 3161 QSH-----------------LRLPLAQGVQVLDL--DRGDENYAEAN----------------PAIRTMPENLAYVIYTS 3205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 266 GSTGKPKGVVHTTGGYLVYAAMTfkyvfdyhpGDIYWCTAD--VGWVTGHSY-----LLYGPLACGAmTLMFEGVPNYPG 338
Cdd:PRK12316 3206 GSTGKPKGVGIRHSALSNHLCWM---------QQAYGLGVGdrVLQFTTFSFdvfveELFWPLMSGA-RVVLAGPEDWRD 3275
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 339 VNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAiegtKRTSLRIMGSVGEPINPEAWEWYynkigNGQCPIVDTWWQTET 418
Cdd:PRK12316 3276 PALLVELINSEGVDVLHAYPSMLQAFLEEEDAH----RCTSLKRIVCGGEALPADLQQQV-----FAGLPLYNLYGPTEA 3346
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 419 GGFMITPLPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSwpGQARTLYGDHD----RFEQTYFSTFKG 494
Cdd:PRK12316 3347 TITVTHWQCVEEGKDAVPIGRPIANRACYILDGSLEPVPVGALGELYLGGE--GLARGYHNRPGltaeRFVPDPFVPGER 3424
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 495 MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVphniKGQAIYAYITLnhgEEPTPE 574
Cdd:PRK12316 3425 LYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVLAV----DGRQLVAYVVP---EDEAGD 3497
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 1206237686 575 LYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGD 622
Cdd:PRK12316 3498 LREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPRPDAAL 3545
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
109-617 |
6.04e-25 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 109.46 E-value: 6.04e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITaDE 188
Cdd:PRK06018 41 TYAQIHDRALKVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFPEQIAWIINHAEDRVVIT-DL 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 189 GMragraIPLKKNVDDALKnpaitSINNVVVFqrTGKTGYWQE--GRDLWWHDLIVGVSADCPAEEMNAEDPLFILYTSG 266
Cdd:PRK06018 120 TF-----VPILEKIADKLP-----SVERYVVL--TDAAHMPQTtlKNAVAYEEWIAEADGDFAWKTFDENTAAGMCYTSG 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 267 STGKPKGVVHTTGGYLVYAAMTFkyvfdyHPGDIYWCTADV-----------GWVTGHSyllyGPlACGAMTLMfegvpn 335
Cdd:PRK06018 188 TTGDPKGVLYSHRSNVLHALMAN------NGDALGTSAADTmlpvvplfhanSWGIAFS----AP-SMGTKLVM------ 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 336 yPGVNRLGQVI----DKHQVNILYTAPTAIRALMAEGDKaiEGTKRTSLRiMGSVGEPINPEAWEWYYNKIGngqCPIVD 411
Cdd:PRK06018 251 -PGAKLDGASVyellDTEKVTFTAGVPTVWLMLLQYMEK--EGLKLPHLK-MVVCGGSAMPRSMIKAFEDMG---VEVRH 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 412 TWWQTETggfmiTPLPGATELKAGSAT--------------RPFFGVQPALVDNLGN--PQEGASEGNLVItdSWPGQAR 475
Cdd:PRK06018 324 AWGMTEM-----SPLGTLAALKPPFSKlpgdarldvlqkqgYPPFGVEMKITDDAGKelPWDGKTFGRLKV--RGPAVAA 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 476 TLY--GDHDRFEQTYFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHN 553
Cdd:PRK06018 397 AYYrvDGEILDDDGFFDT-------GDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHP 469
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1206237686 554 IKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:PRK06018 470 KWDERPLLIVQLKPGETATRE---EILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALRE 530
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
82-615 |
8.28e-25 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 108.18 E-value: 8.28e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTE----RGEQTAIIwegDDPSQskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGA 157
Cdd:cd05920 17 LGDLLARsaarHPDRIAVV---DGDRR---LTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGA 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 158 VHSVIFGGFSPDAVAGRIIDSNAKLVITADEgmragraiplkknvddalknpaitsinnvvvfqrtgktgywQEGRDlwW 237
Cdd:cd05920 91 VPVLALPSHRRSELSAFCAHAEAVAYIVPDR-----------------------------------------HAGFD--H 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 238 HDLIVGVSADCPaeemnaeDPLFILYTSGSTGKPKGVVHTTGGYlVYAAMTFKYVFDYHPGDIYWCTADVGwvtgHSYLL 317
Cdd:cd05920 128 RALARELAESIP-------EVALFLLSGGTTGTPKLIPRTHNDY-AYNVRASAEVCGLDQDTVYLAVLPAA----HNFPL 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 318 YGPLACGamTLMFEG---VPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKaiEGTKRTSLRIMGSVGEPINPEA 394
Cdd:cd05920 196 ACPGVLG--TLLAGGrvvLAPDPSPDAAFPLIEREGVTVTALVPALVSLWLDAAAS--RRADLSSLRLLQVGGARLSPAL 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 395 WEWYYNKIGngqcPIVDTWWQTETGGFMITPLPGATELKAGSATRPffgVQP----ALVDNLGNPQEGASEGNLVITDsw 470
Cdd:cd05920 272 ARRVPPVLG----CTLQQVFGMAEGLLNYTRLDDPDEVIIHTQGRP---MSPddeiRVVDEEGNPVPPGEEGELLTRG-- 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 471 PGQARTLYGDHDRFEQTYfsTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGV 550
Cdd:cd05920 343 PYTIRGYYRAPEHNARAF--TPDGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAM 420
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1206237686 551 PHNIKGQAIYAYITLNhgeePTPELYTEVRNWVRkEIGpIAT---PDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd05920 421 PDELLGERSCAFVVLR----DPPPSAAQLRRFLR-ERG-LAAyklPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
261-616 |
1.69e-24 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 107.08 E-value: 1.69e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 261 ILYTSGSTGKPKGVVHTTGGYLVYAA--MTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMfegvPNYPG 338
Cdd:cd05929 130 MLYSGGTTGRPKGIKRGLPGGPPDNDtlMAAALGFGPGADSVYLSPAPLYHAAPFRWSMTALFMGGTLVLM----EKFDP 205
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 339 VNRLgQVIDKHQVNILYTAPTAIRALMAegdkaIEGTKR-----TSLRIMGSVGEPINPEA------------WEWYYNK 401
Cdd:cd05929 206 EEFL-RLIERYRVTFAQFVPTMFVRLLK-----LPEAVRnaydlSSLKRVIHAAAPCPPWVkeqwidwggpiiWEYYGGT 279
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 402 IGNGQCPIVDTWWQTEtggfmitplpgatelkAGSATRPFFGVQpALVDNLGNPQEGASEGNLVITDSWPgqartlYGDH 481
Cdd:cd05929 280 EGQGLTIINGEEWLTH----------------PGSVGRAVLGKV-HILDEDGNEVPPGEIGEVYFANGPG------FEYT 336
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 482 DRFEQTYFSTFKGMYFS-GDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIY 560
Cdd:cd05929 337 NDPEKTAAARNEGGWSTlGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVH 416
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 561 AYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:cd05929 417 AVVQPAPGADAGTALAEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLR 472
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
107-628 |
1.88e-24 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 107.92 E-value: 1.88e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 107 HVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITA 186
Cdd:PRK06155 46 RWTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALRGPQLEHILRNSGARLLVVE 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 187 DEGMRAGRAIPlkknvDDALKNPAITSINNVVvfqrtgkTGYWQEGrdlWWHDLIVGVSADCPAEEMNAEDPLFILYTSG 266
Cdd:PRK06155 126 AALLAALEAAD-----PGDLPLPAVWLLDAPA-------SVSVPAG---WSTAPLPPLDAPAPAAAVQPGDTAAILYTSG 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 267 STGKPKGVVHTTGGYLVYAAMTFKyVFDYHPGDIYWCTADVGWVTGHSyLLYGPLACGAmTLMFEgvPNYpGVNRLGQVI 346
Cdd:PRK06155 191 TTGPSKGVCCPHAQFYWWGRNSAE-DLEIGADDVLYTTLPLFHTNALN-AFFQALLAGA-TYVLE--PRF-SASGFWPAV 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 347 DKHQVNILYTAPTAIRALMAEGDKAIEGTKRtsLRIMGSVGEPinPEAWEWYYNKIGngqCPIVDTWWQTETGGFMITPL 426
Cdd:PRK06155 265 RRHGATVTYLLGAMVSILLSQPARESDRAHR--VRVALGPGVP--AALHAAFRERFG---VDLLDGYGSTETNFVIAVTH 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 427 PgatELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSWPGQARTLYgdhDRFEQTYFSTFKGMYF-SGDGARRD 505
Cdd:PRK06155 338 G---SQRPGSMGRLAPGFEARVVDEHDQELPDGEPGELLLRADEPFAFATGY---FGMPEKTVEAWRNLWFhTGDRVVRD 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 506 EDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRK 585
Cdd:PRK06155 412 ADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEDEVMAAVVLRDGTALEPV---ALVRHCEP 488
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 1206237686 586 EIGPIATPDILHWTDSLPKTRSGKIMRRILRkiAAGDTSNLGD 628
Cdd:PRK06155 489 RLAYFAVPRYVEFVAALPKTENGKVQKFVLR--EQGVTADTWD 529
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
90-580 |
3.27e-24 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 107.27 E-value: 3.27e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 90 GEQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPD 169
Cdd:PRK08279 51 PDRPALLFED------QSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVALLNTQQRGA 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 170 AVAGRIIDSNAKLVITADEgmragraipLKKNVDDALKNPAITsinnVVVFQRTGKTGYWQEG-RDLwwHDLIVGVSADC 248
Cdd:PRK08279 125 VLAHSLNLVDAKHLIVGEE---------LVEAFEEARADLARP----PRLWVAGGDTLDDPEGyEDL--AAAAAGAPTTN 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 249 PA--EEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMtFKYVFDYHPGDIYWCT--------ADVGWVTGhsylly 318
Cdd:PRK08279 190 PAsrSGVTAKDTAFYIYTSGTTGLPKAAVMSHMRWLKAMGG-FGGLLRLTPDDVLYCClplyhntgGTVAWSSV------ 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 319 gpLACGAmTLmfegvpnypgvnrlgqVIDKH--------QVnILYTApTAI-------RALMAEGDKAIEgtKRTSLRIM 383
Cdd:PRK08279 263 --LAAGA-TL----------------ALRRKfsasrfwdDV-RRYRA-TAFqyigelcRYLLNQPPKPTD--RDHRLRLM 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 384 gsvgepinpeawewyynkIGNGQCPivDTW--WQTETGGFMITPLPGATEL---------KAGSATR-PFFGVQP-ALV- 449
Cdd:PRK08279 320 ------------------IGNGLRP--DIWdeFQQRFGIPRILEFYAASEGnvgfinvfnFDGTVGRvPLWLAHPyAIVk 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 450 ----------DNLGNPQE-GASE-GNLV--ITDSWP--GqartlYGD---------HDRFEQ--TYFSTfkgmyfsGDGA 502
Cdd:PRK08279 380 ydvdtgepvrDADGRCIKvKPGEvGLLIgrITDRGPfdG-----YTDpeasekkilRDVFKKgdAWFNT-------GDLM 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 503 RRDEDGYYWITGRVDDVL-----NVSghrlgTAEIESALVSHPKIAEAAVVGVP-HNIKGQAIYAYITLNHGEEPTP-EL 575
Cdd:PRK08279 448 RDDGFGHAQFVDRLGDTFrwkgeNVA-----TTEVENALSGFPGVEEAVVYGVEvPGTDGRAGMAAIVLADGAEFDLaAL 522
|
....*
gi 1206237686 576 YTEVR 580
Cdd:PRK08279 523 AAHLY 527
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
106-617 |
5.03e-24 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 106.60 E-value: 5.03e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 106 KHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVhsviFGGFSPDAVAGRIID----SNAK 181
Cdd:PLN02330 54 KAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGV----FSGANPTALESEIKKqaeaAGAK 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 182 LVITADEGMRAGRAIPLkknvddalknPAI----TSINNVVVfqrtgktgywqegrdlwWHDLIVGV--SADCPA-EEMN 254
Cdd:PLN02330 130 LIVTNDTNYGKVKGLGL----------PVIvlgeEKIEGAVN-----------------WKELLEAAdrAGDTSDnEEIL 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 255 AEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIywctADVGWVTghSYLLYGPLACGAMTLMFEG-- 332
Cdd:PLN02330 183 QTDLCALPFSSGTTGISKGVMLTHRNLVANLCSSLFSVGPEMIGQV----VTLGLIP--FFHIYGITGICCATLRNKGkv 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 333 --VPNYPGVNRLGQVIdKHQVNILYTAPTAIRALMAegDKAIEGTKRTSLRIMG--SVGEPINPEAWEWYYNKIGNGQcp 408
Cdd:PLN02330 257 vvMSRFELRTFLNALI-TQEVSFAPIVPPIILNLVK--NPIVEEFDLSKLKLQAimTAAAPLAPELLTAFEAKFPGVQ-- 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 409 IVDTWWQTETGgfMITPLPGATELKAGSATRPFFG-VQPALVDNLGNPQEGASegnlvITDSWPGQ--ART---LYGDHD 482
Cdd:PLN02330 332 VQEAYGLTEHS--CITLTHGDPEKGHGIAKKNSVGfILPNLEVKFIDPDTGRS-----LPKNTPGElcVRSqcvMQGYYN 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 483 RFEQTYFSTFK-GMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYA 561
Cdd:PLN02330 405 NKEETDRTIDEdGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGEIPAA 484
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 562 YITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:PLN02330 485 CVVINPKAKESEE---DILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKE 537
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
84-620 |
8.80e-24 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 105.32 E-value: 8.80e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 84 RHLTERGEQTAI-IWEGDdpsqskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVhsvi 162
Cdd:cd05918 7 ERARSQPDAPAVcAWDGS-------LTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGA---- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 163 FGGFSPDAVAGR----IIDSNAKLVITADegmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwh 238
Cdd:cd05918 76 FVPLDPSHPLQRlqeiLQDTGAKVVLTSS--------------------------------------------------- 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 239 dlivgvsadcpaeemnAEDPLFILYTSGSTGKPKGVV--HTTggyLVYAAMTFKYVFDYHPG-----------DIywCTA 305
Cdd:cd05918 105 ----------------PSDAAYVIFTSGSTGKPKGVVieHRA---LSTSALAHGRALGLTSEsrvlqfasytfDV--SIL 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 306 DVgwvtghsyllYGPLACGAMTLmfegVP-NYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDkaiegtkrTSLRIMG 384
Cdd:cd05918 164 EI----------FTTLAAGGCLC----IPsEEDRLNDLAGFINRLRVTWAFLTPSVARLLDPEDV--------PSLRTLV 221
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 385 SVGEPINPEawewyynkigngqcpIVDTWWQ----------TETGGFMITPLPGATElKAGSATRPfFGVQPALVDNlGN 454
Cdd:cd05918 222 LGGEALTQS---------------DVDTWADrvrlinaygpAECTIAATVSPVVPST-DPRNIGRP-LGATCWVVDP-DN 283
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 455 PQE----GAsEGNLVItdSWPGQARtlyGDHDRFEQTYFSTFKG--------------MYFSGDGARRDEDG--YYwiTG 514
Cdd:cd05918 284 HDRlvpiGA-VGELLI--EGPILAR---GYLNDPEKTAAAFIEDpawlkqegsgrgrrLYRTGDLVRYNPDGslEY--VG 355
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 515 RVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQA---IYAYITLNHGE-----------EPTPELY---T 577
Cdd:cd05918 356 RKDTQVKIRGQRVELGEIEHHLRQSLPGAKEVVVEVVKPKDGSSspqLVAFVVLDGSSsgsgdgdslflEPSDEFRalvA 435
|
570 580 590 600
....*....|....*....|....*....|....*....|...
gi 1206237686 578 EVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAA 620
Cdd:cd05918 436 ELRSKLRQRLPSYMVPSVFLPLSHLPLTASGKIDRRALRELAE 478
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
106-617 |
1.03e-23 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 107.35 E-value: 1.03e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 106 KHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVIT 185
Cdd:PRK12316 2027 QHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLLT 2106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 186 aDEGMRAGRAIPlkknvdDALKNPAITsinnvvvfqrtgktgywqegRDLWWHDLIVGVsadcPAEEMNAEDPLFILYTS 265
Cdd:PRK12316 2107 -QRHLLERLPLP------AGVARLPLD--------------------RDAEWADYPDTA----PAVQLAGENLAYVIYTS 2155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 266 GSTGKPKGVVHTTGGYLVYAAMTFKYvFDYHPGDIYWCTADVGWVTGHSYLLYgPLACGAMTLMFEGVPNYPGvnRLGQV 345
Cdd:PRK12316 2156 GSTGLPKGVAVSHGALVAHCQAAGER-YELSPADCELQFMSFSFDGAHEQWFH-PLLNGARVLIRDDELWDPE--QLYDE 2231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 346 IDKHQVNILYTAPTAIRALMAEgdKAIEGtKRTSLRIMGSVGEPINPEAWEWYYNKIGNGQcpIVDTWWQTETggfMITP 425
Cdd:PRK12316 2232 MERHGVTILDFPPVYLQQLAEH--AERDG-RPPAVRVYCFGGEAVPAASLRLAWEALRPVY--LFNGYGPTEA---VVTP 2303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 426 LP-GATELKAGSATRPFFGvqpALVDNLGN----------PQEGAseGNLVITDSwpGQARtlyGDHDRFEQT------- 487
Cdd:PRK12316 2304 LLwKCRPQDPCGAAYVPIG---RALGNRRAyildadlnllAPGMA--GELYLGGE--GLAR---GYLNRPGLTaerfvpd 2373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 488 -YFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVpHNIKGQAIYAYITln 566
Cdd:PRK12316 2374 pFSASGERLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAVVVAQ-DGASGKQLVAYVV-- 2450
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 567 hGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:PRK12316 2451 -PDDAAEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPK 2500
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
105-615 |
1.36e-23 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 107.17 E-value: 1.36e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 105 SKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVI 184
Cdd:PRK12467 1597 EQELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLL 1676
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 185 TadegmragraiplKKNVDDALknPAITSINNVVVFQrtgkTGYWQEGRDLwwHDLIVGVSADCPAeemnaedplFILYT 264
Cdd:PRK12467 1677 T-------------QSHLQARL--PLPDGLRSLVLDQ----EDDWLEGYSD--SNPAVNLAPQNLA---------YVIYT 1726
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 265 SGSTGKPKGVVHTTGGYL-VYAAMTFKYVFdyhpgdiywCTADVgWVTGHSYL-------LYGPLACGAMTLMfegVPny 336
Cdd:PRK12467 1727 SGSTGRPKGAGNRHGALVnRLCATQEAYQL---------SAADV-VLQFTSFAfdvsvweLFWPLINGARLVI---AP-- 1791
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 337 PGVNR----LGQVIDKHQVNILYTAPTAIRALMaEGDKAIEGTkrTSLRIMGSVGEPINPEAWEWYYNKIGNGQcpIVDT 412
Cdd:PRK12467 1792 PGAHRdpeqLIQLIERQQVTTLHFVPSMLQQLL-QMDEQVEHP--LSLRRVVCGGEALEVEALRPWLERLPDTG--LFNL 1866
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 413 WWQTETggfMITPLPGATELK--AGSATRPfFGVQPA-----LVDNLGNPQEGASEGNLVITDSwpGQARtlyGDH---- 481
Cdd:PRK12467 1867 YGPTET---AVDVTHWTCRRKdlEGRDSVP-IGQPIAnlstyILDASLNPVPIGVAGELYLGGV--GLAR---GYLnrpa 1937
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 482 ---DRFEQTYFSTFKG-MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVpHNIKGQ 557
Cdd:PRK12467 1938 ltaERFVADPFGTVGSrLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVIAQ-DGANGK 2016
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1206237686 558 AIYAYIT-----LNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:PRK12467 2017 QLVAYVVptdpgLVDDDEAQVALRAILKNHLKASLPEYMVPAHLVFLARMPLTPNGKLDRKAL 2079
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
82-620 |
1.52e-23 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 104.89 E-value: 1.52e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIwegdDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:PRK08315 22 LDRTAARYPDREALV----YRDQGLRWTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAILVT 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVITADE-------GMRAGRAIPLKKNVDDALKNPAITSINNVVVFQRTGKTGYWQegrd 234
Cdd:PRK08315 98 INPAYRLSELEYALNQSGCKALIAADGfkdsdyvAMLYELAPELATCEPGQLQSARLPELRRVIFLGDEKHPGMLN---- 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 235 lwWHDLIVGVSADCPAE------EMNAEDPLFILYTSGSTGKPKGVVHT-----TGGYLVYAAMTFKyvfdyhPGD---- 299
Cdd:PRK08315 174 --FDELLALGRAVDDAElaarqaTLDPDDPINIQYTSGTTGFPKGATLThrnilNNGYFIGEAMKLT------EEDrlci 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 300 ---IYWCtadVGWVTGHsyllygpLAC----GAMTLMFEGvpnYPGVNRLgQVIDKHQVNILYTAPTairalM--AE-GD 369
Cdd:PRK08315 246 pvpLYHC---FGMVLGN-------LACvthgATMVYPGEG---FDPLATL-AAVEEERCTALYGVPT-----MfiAElDH 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 370 KAIEGTKRTSLR--IM-GSVgepinpeawewyynkigngqCPI------VD---------TWWQTETGgfmitplPGAT- 430
Cdd:PRK08315 307 PDFARFDLSSLRtgIMaGSP--------------------CPIevmkrvIDkmhmsevtiAYGMTETS-------PVSTq 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 431 -------ELKAGSATRPFFGVQPALVDNL-------GNPQEGASEGNLVITDSWPGQARTLYG-DHDRFeqtyfstfkgM 495
Cdd:PRK08315 360 trtddplEKRVTTVGRALPHLEVKIVDPEtgetvprGEQGELCTRGYSVMKGYWNDPEKTAEAiDADGW----------M 429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 496 YfSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPEl 575
Cdd:PRK08315 430 H-TGDLAVMDEEGYVNIVGRIKDMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTEE- 507
|
570 580 590 600
....*....|....*....|....*....|....*....|....*...
gi 1206237686 576 ytEVRNWVRkeiGPIA---TPDILHWTDSLPKTRSGKIMRRILRKIAA 620
Cdd:PRK08315 508 --DVRDFCR---GKIAhykIPRYIRFVDEFPMTVTGKIQKFKMREMMI 550
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
103-616 |
1.61e-23 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 104.78 E-value: 1.61e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 103 SQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAK- 181
Cdd:PRK12406 7 SGDRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARv 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 182 LVITADEGMRAGRAIPLKKNVDDALKNPAITS---INNVVVFQRTGKTGYwqegrDLWwhdlivgVSADCPAEEMNAEDP 258
Cdd:PRK12406 87 LIAHADLLHGLASALPAGVTVLSVPTPPEIAAayrISPALLTPPAGAIDW-----EGW-------LAQQEPYDGPPVPQP 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 259 LFILYTSGSTGKPKGVVH---TTGGYLVYAAMTfkyvfdyhpGDIYwctadvGWVTGHSYLLYGPlacgamtlMFEGVPN 335
Cdd:PRK12406 155 QSMIYTSGTTGHPKGVRRaapTPEQAAAAEQMR---------ALIY------GLKPGIRALLTGP--------LYHSAPN 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 336 YPGVN--RLG---------------QVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPinpeawewy 398
Cdd:PRK12406 212 AYGLRagRLGgvlvlqprfdpeellQLIERHRITHMHMVPTMFIRLLKLPEEVRAKYDVSSLRHVIHAAAP--------- 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 399 ynkigngqCP------IVDtWWqtetgGFMITPLPGATEL-------------KAGSATRPFFGVQPALVDNLGNPQEGA 459
Cdd:PRK12406 283 --------CPadvkraMIE-WW-----GPVIYEYYGSTESgavtfatsedalsHPGTVGKAAPGAELRFVDEDGRPLPQG 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 460 SEGNLVITDswPGQARTLYgdHDRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSH 539
Cdd:PRK12406 349 EIGEIYSRI--AGNPDFTY--HNKPEKRAEIDRGGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAV 424
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1206237686 540 PKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK12406 425 PGVHDCAVFGIPDAEFGEALMAVVEPQPGATLDEA---DIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLR 498
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
109-621 |
1.78e-23 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 105.10 E-value: 1.78e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADE 188
Cdd:PLN03102 41 TWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFVDRS 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 189 GMRAGRAIPLKKNVDDALKNPAITSINNVVVFQRTGKtgywqegRDLWWHDLIV-GVSADCPAEEM----NAEDPLFILY 263
Cdd:PLN03102 121 FEPLAREVLHLLSSEDSNLNLPVIFIHEIDFPKRPSS-------EELDYECLIQrGEPTPSLVARMfriqDEHDPISLNY 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 264 TSGSTGKPKGVVHT-TGGYLVYAAMTFKYVFDYHPgdIYWCTADV----GWVtghsyLLYGPLACGAMTLMFEGVpNYPG 338
Cdd:PLN03102 194 TSGTTADPKGVVIShRGAYLSTLSAIIGWEMGTCP--VYLWTLPMfhcnGWT-----FTWGTAARGGTSVCMRHV-TAPE 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 339 VNRlgqVIDKHQVNILYTAPTAIRALMaEGDKAIEGTKRTSLRIMGSVGEPinPEAWEWYYNKIG----------NGQCP 408
Cdd:PLN03102 266 IYK---NIEMHNVTHMCCVPTVFNILL-KGNSLDLSPRSGPVHVLTGGSPP--PAALVKKVQRLGfqvmhaygltEATGP 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 409 IVDTWWQTETGGFmitPLPGATELKAGSATRpFFGVQPALVDNLGN----PQEGASEGNLVITDSwpgqarTLYGDHDRF 484
Cdd:PLN03102 340 VLFCEWQDEWNRL---PENQQMELKARQGVS-ILGLADVDVKNKETqesvPRDGKTMGEIVIKGS------SIMKGYLKN 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 485 EQTYFSTFK-GMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYI 563
Cdd:PLN03102 410 PKATSEAFKhGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPCAFV 489
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1206237686 564 TLNHGEEPTPE----LYTEVRN---WVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAG 621
Cdd:PLN03102 490 VLEKGETTKEDrvdkLVTRERDlieYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLRDIAKG 554
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
257-612 |
1.91e-23 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 101.71 E-value: 1.91e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 257 DPLFILYTSGSTGKPKGVVHTTGGYLVYaamtfkyvFDYHPGDIYWCTADVGWVTG---HSYLLYgplacGAMTLMFEG- 332
Cdd:cd17633 1 NPFYIGFTSGTTGLPKAYYRSERSWIES--------FVCNEDLFNISGEDAILAPGplsHSLFLY-----GAISALYLGg 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 333 ---VPNYPGVNRLGQVIDKHQVNILYTAPTAIRALmaegdkAIEGTKRTSLRIMGSVGEPINPEAWEwyynKIGNG--QC 407
Cdd:cd17633 68 tfiGQRKFNPKSWIRKINQYNATVIYLVPTMLQAL------ARTLEPESKIKSIFSSGQKLFESTKK----KLKNIfpKA 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 408 PIVDTWWQTETGgfMITPLPGATELKAGSATRPFFGVQPALVDNLGNPQ-----------EGASEGNLVITDSWpgqart 476
Cdd:cd17633 138 NLIEFYGTSELS--FITYNFNQESRPPNSVGRPFPNVEIEIRNADGGEIgkifvksemvfSGYVRGGFSNPDGW------ 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 477 lygdhdrfeqtyFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKG 556
Cdd:cd17633 210 ------------MSV-------GDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFG 270
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 557 QAIYAYITlnhGEEPTpelYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMR 612
Cdd:cd17633 271 EIAVALYS---GDKLT---YKQLKRFLKQKLSRYEIPKKIIFVDSLPYTSSGKIAR 320
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
237-616 |
3.19e-23 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 103.95 E-value: 3.19e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 237 WHDLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYA-AMTFKYvfDYHPGDIYWCTADVGwvtgHS- 314
Cdd:PRK13388 131 YAELVAAAGALTPHREVDAMDPFMLIFTSGTTGAPKAVRCSHGRLAFAGrALTERF--GLTRDDVCYVSMPLF----HSn 204
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 315 --YLLYGP-LACGA---MTLMFEGVPNYPGVNRLGqvidkhqVNILYTAPTAIRALMAEGDKAIEGTkrTSLRImgSVGE 388
Cdd:PRK13388 205 avMAGWAPaVASGAavaLPAKFSASGFLDDVRRYG-------ATYFNYVGKPLAYILATPERPDDAD--NPLRV--AFGN 273
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 389 PINPEAWEWYYNKIGngqCPIVDTWWQTETGGfMITPLPGATElkaGSATRPFFGVQ-----------PALVDNLG---N 454
Cdd:PRK13388 274 EASPRDIAEFSRRFG---CQVEDGYGSSEGAV-IVVREPGTPP---GSIGRGAPGVAiynpetltecaVARFDAHGallN 346
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 455 PQEGAseGNLVITDSwPGQARTLYGDHD----RFEQtyfstfkGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTA 530
Cdd:PRK13388 347 ADEAI--GELVNTAG-AGFFEGYYNNPEataeRMRH-------GMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAA 416
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 531 EIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNwVRKEIGPIATPDILHWTDSLPKTRSGKI 610
Cdd:PRK13388 417 PIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGATFDPDAFAAFLA-AQPDLGTKAWPRYVRIAADLPSTATNKV 495
|
....*.
gi 1206237686 611 MRRILR 616
Cdd:PRK13388 496 LKRELI 501
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
256-612 |
4.19e-23 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 101.18 E-value: 4.19e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 256 EDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDyhpgdiyWCTADVGWVTGHSYLLYGpLACGAMTLMFEGV-- 333
Cdd:cd17635 1 EDPLAVIFTSGTTGEPKAVLLANKTFFAVPDILQKEGLN-------WVVGDVTYLPLPATHIGG-LWWILTCLIHGGLcv 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 334 --PNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKrtSLRIMGSVGE-PINPEAWEWYYNKIGNgqcpIV 410
Cdd:cd17635 73 tgGENTTYKSLFKILTTNAVTTTCLVPTLLSKLVSELKSANATVP--SLRLIGYGGSrAIAADVRFIEATGLTN----TA 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 411 DTWWQTETGGFMITPLpGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSWpgqarTLYGDHDRFEQTYFS 490
Cdd:cd17635 147 QVYGLSETGTALCLPT-DDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPA-----NMLGYWNNPERTAEV 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 491 TFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNhgEE 570
Cdd:cd17635 221 LIDGWVNTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVAS--AE 298
|
330 340 350 360
....*....|....*....|....*....|....*....|..
gi 1206237686 571 PTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMR 612
Cdd:cd17635 299 LDENAIRALKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
251-616 |
6.73e-23 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 102.38 E-value: 6.73e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 251 EEMNAEDPLFILYTSGSTGKPKGVVhttggyLVYAAMTfkyvfdyhpGDI-------YWCTADV-----------GWVTG 312
Cdd:PRK07787 123 PEPDPDAPALIVYTSGTTGPPKGVV------LSRRAIA---------ADLdalaeawQWTADDVlvhglplfhvhGLVLG 187
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 313 hsylLYGPLACGAmTLMFEGVPNYPGVNRLGQVidkhQVNILYTAPTAIRALMAEGDKAiEGTKRTSLRIMGSVGEPInP 392
Cdd:PRK07787 188 ----VLGPLRIGN-RFVHTGRPTPEAYAQALSE----GGTLYFGVPTVWSRIAADPEAA-RALRGARLLVSGSAALPV-P 256
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 393 EawewyYNKIG--NGQcPIVDTWWQTETggFMITPLPGATELKAGSATRPFFGVQPALVDNLGN--PQEGASEGNLVItd 468
Cdd:PRK07787 257 V-----FDRLAalTGH-RPVERYGMTET--LITLSTRADGERRPGWVGLPLAGVETRLVDEDGGpvPHDGETVGELQV-- 326
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 469 swpgQARTLY-GDHDRFEQTYFS-TFKGMYFSGDGARRDEDGYYWITGRVD-DVLNVSGHRLGTAEIESALVSHPKIAEA 545
Cdd:PRK07787 327 ----RGPTLFdGYLNRPDATAAAfTADGWFRTGDVAVVDPDGMHRIVGREStDLIKSGGYRIGAGEIETALLGHPGVREA 402
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 546 AVVGVPHNIKGQAIYAYITlnHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK07787 403 AVVGVPDDDLGQRIVAYVV--GADDVAAD---ELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLL 468
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
91-617 |
1.00e-22 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 102.12 E-value: 1.00e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWeGDDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDA 170
Cdd:cd05915 9 GRKEVVS-RLHTGEVHRTTYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKE 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 171 VAGRIIDSNAKLVITADEGMR-AGRAIPLKKNVDDalkNPaitsinnvvvfqrTGKTGYWQegrdlwWHDLI-VGVSADC 248
Cdd:cd05915 88 IAYILNHAEDKVLLFDPNLLPlVEAIRGELKTVQH---FV-------------VMDEKAPE------GYLAYeEALGEEA 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 249 PAEEMNAEDPLFILYTSGSTGKPKGVVHT-TGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLlYGPLACGAMT 327
Cdd:cd05915 146 DPVRVPERAACGMAYTTGTTGLPKGVVYShRALVLHSLAASLVDGTALSEKDVVLPVVPMFHVNAWCLP-YAATLVGAKQ 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 328 LMFEGVPNYpgvNRLGQVIDKHQVNILYTAPTAIRaLMAEGDKAIEGTKRTSLRIMGSVGEPinPEAWeWYYNKIGNGQ- 406
Cdd:cd05915 225 VLPGPRLDP---ASLVELFDGEGVTFTAGVPTVWL-ALADYLESTGHRLKTLRRLVVGGSAA--PRSL-IARFERMGVEv 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 407 -----CPIV-----DTWWQTEtggFMITPLPGATELKAGSATRPFFGVQPAL-VDNLGNPQEG------ASEGNLVITDs 469
Cdd:cd05915 298 rqgygLTETspvvvQNFVKSH---LESLSEEEKLTLKAKTGLPIPLVRLRVAdEEGRPVPKDGkalgevQLKGPWITGG- 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 470 wpgqartLYGDHDRFEQTYFStfKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVG 549
Cdd:cd05915 374 -------YYGNEEATRSALTP--DGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVA 444
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 550 VPHNIKGQAIYAYITLNHGEEPTPELYtevrNWVRKEIGPIAT-PDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:cd05915 445 IPHPKWQERPLAVVVPRGEKPTPEELN----EHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALRE 509
|
|
| ACAS_N |
pfam16177 |
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many ... |
24-81 |
1.22e-22 |
|
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many acetyl-coenzyme A synthetase enzymes.
Pssm-ID: 465043 [Multi-domain] Cd Length: 55 Bit Score: 90.99 E-value: 1.22e-22
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*...
gi 1206237686 24 YHDFYQQSVQNPDAFWGEQGKIIDWIKPYSIVKNTSFDPGhvsIRWFEDGTLNLSANC 81
Cdd:pfam16177 1 YEALYRRSIEDPEGFWGEVAKELDWFKPFDKVLDGSNGPF---AKWFVGGKLNVCYNC 55
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
108-619 |
3.45e-22 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 100.48 E-value: 3.45e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKlGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITAD 187
Cdd:cd05909 8 LTYRKLLTGAIALARKLAK-MTKEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAGIKTVLTSK 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 EGMRAGRAIPLKknvdDALKNPAITSINNVvvfqRTGKTgywqegrdlWWHDLIVGVSADCPAEEM---------NAEDP 258
Cdd:cd05909 87 QFIEKLKLHHLF----DVEYDARIVYLEDL----RAKIS---------KADKCKAFLAGKFPPKWLlrifgvapvQPDDP 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 259 LFILYTSGSTGKPKGVVHTTGGYL--VYAAMTfkyVFDYHPGDIywctadvgwVTG-----HSYLLYG----PLACGAMT 327
Cdd:cd05909 150 AVILFTSGSEGLPKGVVLSHKNLLanVEQITA---IFDPNPEDV---------VFGalpffHSFGLTGclwlPLLSGIKV 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 328 LMFEGVPNYPGVNRLgqvIDKHQVNILYTAPTAIRALMaegdKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGngqC 407
Cdd:cd05909 218 VFHPNPLDYKKIPEL---IYDKKATILLGTPTFLRGYA----RAAHPEDFSSLRLVVAGAEKLKDTLRQEFQEKFG---I 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 408 PIVDTWWQTETGGF--MITPLPGAtelKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSwPGQARTLYGDHdrfE 485
Cdd:cd05909 288 RILEGYGTTECSPVisVNTPQSPN---KEGTVGRPLPGMEVKIVSVETHEEVPIGEGGLLLVRG-PNVMLGYLNEP---E 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 486 QTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSH-PKIAEAAVVGVPHNIKGQAIYAYIT 564
Cdd:cd05909 361 LTSFAFGDGWYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEIlPEDNEVAVVSVPDGRKGEKIVLLTT 440
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 565 lnhGEEPTPElytEVRNWVRK-EIGPIATPDILHWTDSLPKTRSGKIMRRILRKIA 619
Cdd:cd05909 441 ---TTDTDPS---SLNDILKNaGISNLAKPSYIHQVEEIPLLGTGKPDYVTLKALA 490
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
76-621 |
4.01e-22 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 100.47 E-value: 4.01e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 76 NLSANCLDRHLTERGEQ-TAIIWEGDDPSQSkhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACAR 154
Cdd:PRK05857 11 QLPSTVLDRVFEQARQQpEAIALRRCDGTSA--LRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAK 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 155 IGAVHSVIFGGFSPDAVA--GRIIDSNAklVITADEGMRAGRAIPlkknvDDALKNPAITsinnvvvFQRTGKTGYWQEG 232
Cdd:PRK05857 89 LGAIAVMADGNLPIAAIErfCQITDPAA--ALVAPGSKMASSAVP-----EALHSIPVIA-------VDIAAVTRESEHS 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 233 RDLwwhdlivgvsaDCPAEEMN--AEDPLFILYTSGSTGKPKGVVhttggylvYAAMTFKYVFD-YHPGDIYWctadVGW 309
Cdd:PRK05857 155 LDA-----------ASLAGNADqgSEDPLAMIFTSGTTGEPKAVL--------LANRTFFAVPDiLQKEGLNW----VTW 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 310 VTGHSylLYGPLAC---GAM-----TLMFEGVPNYPGVN--RLGQVIDKHQVNILYTAPTAIRALMAEGDKAieGTKRTS 379
Cdd:PRK05857 212 VVGET--TYSPLPAthiGGLwwiltCLMHGGLCVTGGENttSLLEILTTNAVATTCLVPTLLSKLVSELKSA--NATVPS 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 380 LRIMGSVGEpinpEAWEWYYNKIGNGQCPIVDTWWQTETGGFMITpLPGATE----LKAGSATRPFFGVQPALVD-NLGN 454
Cdd:PRK05857 288 LRLVGYGGS----RAIAADVRFIEATGVRTAQVYGLSETGCTALC-LPTDDGsivkIEAGAVGRPYPGVDVYLAAtDGIG 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 455 PQ-----EGASEGNLvitdsWPGQARTLYGDHDRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGT 529
Cdd:PRK05857 363 PTapgagPSASFGTL-----WIKSPANMLGYWNNPERTAEVLIDGWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAP 437
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 530 AEIESALVSHPKIAEAAVVGVPHN----IKGQAIYAYITLNhgEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKT 605
Cdd:PRK05857 438 DEVDRIAEGVSGVREAACYEIPDEefgaLVGLAVVASAELD--ESAARALKHTIAARFRRESEPMARPSTIVIVTDIPRT 515
|
570
....*....|....*.
gi 1206237686 606 RSGKIMRRILRKIAAG 621
Cdd:PRK05857 516 QSGKVMRASLAAAATA 531
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
48-625 |
4.90e-22 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 100.30 E-value: 4.90e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 48 WIKPYSIVKNTSFDPGHVSIrwfeDGTLNLSANCLDRHltERGEQTAIIwegdDPSQSKHVTYKQLHHNVCQFANVL-KK 126
Cdd:PLN02574 17 WYSPETGIYSSKHPPVPLPS----DPNLDAVSFIFSHH--NHNGDTALI----DSSTGFSISYSELQPLVKSMAAGLyHV 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 127 LGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADEgmragraiplkkNVDD-- 204
Cdd:PLN02574 87 MGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGLAFTSPE------------NVEKls 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 205 ALKNPAITSINNvvvfqrtgktGYWQEGRDLW--WHDLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYL 282
Cdd:PLN02574 155 PLGVPVIGVPEN----------YDFDSKRIEFpkFYELIKEDFDFVPKPVIKQDDVAAIMYSSGTTGASKGVVLTHRNLI 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 283 VYAAMTFKY---VFDYHPGD-IYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVpnypGVNRLGQVIDKHQVNILYTAP 358
Cdd:PLN02574 225 AMVELFVRFeasQYEYPGSDnVYLAALPMFHIYGLSLFVVGLLSLGSTIVVMRRF----DASDMVKVIDRFKVTHFPVVP 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 359 TAIRALMAEGdKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIgngqcPIVDTwwqteTGGFmitplpGATElKAGSAT 438
Cdd:PLN02574 301 PILMALTKKA-KGVCGEVLKSLKQVSCGAAPLSGKFIQDFVQTL-----PHVDF-----IQGY------GMTE-STAVGT 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 439 RPFfgvqpalvdNLGNPQEGASEGNLV----------ITDSW--PGQARTLYGDHDRFEQTYFSTFKGMYFS-------- 498
Cdd:PLN02574 363 RGF---------NTEKLSKYSSVGLLApnmqakvvdwSTGCLlpPGNCGELWIQGPGVMKGYLNNPKATQSTidkdgwlr 433
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 499 -GDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElyt 577
Cdd:PLN02574 434 tGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGSTLSQE--- 510
|
570 580 590 600
....*....|....*....|....*....|....*....|....*...
gi 1206237686 578 EVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIAAGDTSN 625
Cdd:PLN02574 511 AVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELKRSLTNSVSS 558
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
79-613 |
8.20e-22 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 99.58 E-value: 8.20e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 79 ANCLDRHLTERGEQTAIIWEgddpSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAV 158
Cdd:PRK05852 19 ADLVEVAATRLPEAPALVVT----ADRIAISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLV 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 159 HSVIFGGFSPDAVAGRIIDSNAKLVITADEGMrAGRAIPLKKNVDDALKNPAITSINNVVVFQRTGKTGywqegrdlwwh 238
Cdd:PRK05852 95 VVPLDPALPIAEQRVRSQAAGARVVLIDADGP-HDRAEPTTRWWPLTVNVGGDSGPSGGTLSVHLDAAT----------- 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 239 DLIVGVSADcpaEEMNAEDPLfILYTSGSTGKPKGVVHTTGGYlvyAAMTFKYVFDYHPGDIYWCTADVGWVTGHsylly 318
Cdd:PRK05852 163 EPTPATSTP---EGLRPDDAM-IMFTGGTTGLPKMVPWTHANI---ASSVRAIITGYRLSPRDATVAVMPLYHGH----- 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 319 GPLACGAMTLMFEGVPNYPGVNRLGQVI---DKHQVN-ILYTA-PTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPE 393
Cdd:PRK05852 231 GLIAALLATLASGGAVLLPARGRFSAHTfwdDIKAVGaTWYTAvPTIHQILLERAATEPSGRKPAALRFIRSCSAPLTAE 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 394 AWEWYYNKIGngqCPIVDTWWQTET---------GGFMITPLPGATELKAGSATrpffGVQPALVDNLGNPQEGASEGNL 464
Cdd:PRK05852 311 TAQALQTEFA---APVVCAFGMTEAthqvtttqiEGIGQTENPVVSTGLVGRST----GAQIRIVGSDGLPLPAGAVGEV 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 465 VItdSWPGQARTLYGDHdrfEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAE 544
Cdd:PRK05852 384 WL--RGTTVVRGYLGDP---TITAANFTDGWLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVME 458
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 545 AAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRR 613
Cdd:PRK05852 459 AAVFGVPDQLYGEAVAAVIVPRESAPPTAE---ELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRR 524
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
256-609 |
8.50e-22 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 97.45 E-value: 8.50e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 256 EDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDyHPGDIYWCTADVGWVTGHSYLLYGPLACG-----AMTLMF 330
Cdd:cd05924 3 ADDLYILYTGGTTGMPKGVMWRQEDIFRMLMGGADFGTG-EFTPSEDAHKAAAAAAGTVMFPAPPLMHGtgswtAFGGLL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 331 EG------VPNYPGVNRLgQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGN 404
Cdd:cd05924 82 GGqtvvlpDDRFDPEEVW-RTIEKHKVTSMTIVGDAMARPLIDALRDAGPYDLSSLFAISSGGALLSPEVKQGLLELVPN 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 405 gqCPIVDTWWQTETGGFMItplpgATELKAGSATRPFFGVQP--ALVDNLGNPQEGASEGnlvitdswPGQ-ART----- 476
Cdd:cd05924 161 --ITLVDAFGSSETGFTGS-----GHSAGSGPETGPFTRANPdtVVLDDDGRVVPPGSGG--------VGWiARRghipl 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 477 -LYGDHDRFEQTYFSTFKGMY-FSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNI 554
Cdd:cd05924 226 gYYGDEAKTAETFPEVDGVRYaVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDER 305
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*
gi 1206237686 555 KGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGK 609
Cdd:cd05924 306 WGQEVVAVVQLREGAGVDLE---ELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
87-622 |
1.23e-21 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 99.09 E-value: 1.23e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 87 TERGEQTAIIWEGDDPSQSkhvTYKQLHHNVCQFANVLK-KLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGG 165
Cdd:PRK05620 21 TVHGDTTVTTWGGAEQEQT---TFAAIGARAAALAHALHdELGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQ 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 166 FSPDAVAgRIIDSNAKLVITADEGMrAGRAIPLKKNVD--DALKNPAITSINNVVVFQRTGKTGYWQEGrdlwwhdLIVG 243
Cdd:PRK05620 98 LMNDQIV-HIINHAEDEVIVADPRL-AEQLGEILKECPcvRAVVFIGPSDADSAAAHMPEGIKVYSYEA-------LLDG 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 244 VSADCPAEEMNAEDPLFILYTSGSTGKPKGVVH-------------TTGGYLVYAAMTFKY-VFDYHPgdIYWCTADVGW 309
Cdd:PRK05620 169 RSTVYDWPELDETTAAAICYSTGTTGAPKGVVYshrslylqslslrTTDSLAVTHGESFLCcVPIYHV--LSWGVPLAAF 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 310 VTGHSYLLYGPLACGAmtlmfegvpnypgvnRLGQVIDKHQVNILYTAPTAIRALMAEGDKaiEGTKRTSLRIMGSVGEP 389
Cdd:PRK05620 247 MSGTPLVFPGPDLSAP---------------TLAKIIATAMPRVAHGVPTLWIQLMVHYLK--NPPERMSLQEIYVGGSA 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 390 INP---EAWEWYYNkigngqCPIVDTWWQTETG--GFMITPLPGAtelkAGSATRPFFGVQ---PA----LVDNLGNPQE 457
Cdd:PRK05620 310 VPPiliKAWEERYG------VDVVHVWGMTETSpvGTVARPPSGV----SGEARWAYRVSQgrfPAsleyRIVNDGQVME 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 458 GA--SEGNLVITDSW-------------PGQARTLYGDHDRFEQTYFsTFKGMYFSGDGARRDEDGYYWITGRVDDVLNV 522
Cdd:PRK05620 380 STdrNEGEIQVRGNWvtasyyhspteegGGAASTFRGEDVEDANDRF-TADGWLRTGDVGSVTRDGFLTIHDRARDVIRS 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 523 SGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSL 602
Cdd:PRK05620 459 GGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTRETAERLRDQLRDRLPNWMLPEYWTFVDEI 538
|
570 580
....*....|....*....|.
gi 1206237686 603 PKTRSGKIMRRILRK-IAAGD 622
Cdd:PRK05620 539 DKTSVGKFDKKDLRQhLADGD 559
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
108-616 |
2.96e-21 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 98.57 E-value: 2.96e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITAd 187
Cdd:PRK06060 31 VTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPELHRDDHALAARNTEPALVVTS- 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 eGMRAGRAIPlkKNVDDA--LKNPAitsinnvvvfQRTGKTGYwqegrdlwwhdlivgvsadcpaEEMNAEDPLFILYTS 265
Cdd:PRK06060 110 -DALRDRFQP--SRVAEAaeLMSEA----------ARVAPGGY----------------------EPMGGDALAYATYTS 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 266 GSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMfEGVPnyPGVNRLGQV 345
Cdd:PRK06060 155 GTTGPPKAAIHRHADPLTFVDAMCRKALRLTPEDTGLCSARMYFAYGLGNSVWFPLATGGSAVI-NSAP--VTPEAAAIL 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 346 IDKHQVNILYTAPTairaLMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNgqCPIVDTWWQTETGGFMITP 425
Cdd:PRK06060 232 SARFGPSVLYGVPN----FFARVIDSCSPDSFRSLRCVVSAGEALELGLAERLMEFFGG--IPILDGIGSTEVGQTFVSN 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 426 lpGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNL-----VITDSWPGQARTLYGDHDRFEqtyfstfkgmyfSGD 500
Cdd:PRK06060 306 --RVDEWRLGTLGRVLPPYEIRVVAPDGTTAGPGVEGDLwvrgpAIAKGYWNRPDSPVANEGWLD------------TRD 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 501 GARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVR 580
Cdd:PRK06060 372 RVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDGSVMRDLH 451
|
490 500 510
....*....|....*....|....*....|....*.
gi 1206237686 581 NWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK06060 452 RGLLNRLSAFKVPHRFAVVDRLPRTPNGKLVRGALR 487
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
108-618 |
8.02e-21 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 96.44 E-value: 8.02e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITAD 187
Cdd:cd17642 45 YSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVFCSK 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 EGmragraipLKKNVDDALKNPAITSInnVVVFQRTGKTGYWQEGRDLWWHDLIVGVSADCPAEEMNAEDPL-FILYTSG 266
Cdd:cd17642 125 KG--------LQKVLNVQKKLKIIKTI--IILDSKEDYKGYQCLYTFITQNLPPGFNEYDFKPPSFDRDEQVaLIMNSSG 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 267 STGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYL-LYGPLACGAMTLMfegVPNYPGVNRLgQV 345
Cdd:cd17642 195 STGLPKGVQLTHKNIVARFSHARDPIFGNQIIPDTAILTVIPFHHGFGMFtTLGYLICGFRVVL---MYKFEEELFL-RS 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 346 IDKHQVNILYTAPTairaLMAEGDKA--IEGTKRTSLRIMGSVGEPINPEAWEWYYNKIG-NGqcpIVDTWWQTE-TGGF 421
Cdd:cd17642 271 LQDYKVQSALLVPT----LFAFFAKStlVDKYDLSNLHEIASGGAPLSKEVGEAVAKRFKlPG---IRQGYGLTEtTSAI 343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 422 MITPlpgATELKAGSATR--PFFGVQpaLVDNLGNPQEGASE-GNLVITDswPGQARTLYGDHdrfEQTYFSTFK-GMYF 497
Cdd:cd17642 344 LITP---EGDDKPGAVGKvvPFFYAK--VVDLDTGKTLGPNErGELCVKG--PMIMKGYVNNP---EATKALIDKdGWLH 413
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 498 SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElyt 577
Cdd:cd17642 414 SGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVVVLEAGKTMTEK--- 490
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 1206237686 578 EVRNWVRKEIGPIA-TPDILHWTDSLPKTRSGKIMRRILRKI 618
Cdd:cd17642 491 EVMDYVASQVSTAKrLRGGVKFVDEVPKGLTGKIDRRKIREI 532
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
104-612 |
1.20e-20 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 95.20 E-value: 1.20e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 104 QSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLV 183
Cdd:cd05914 4 GGEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAI 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 184 ITADEgmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemnaEDPLFILY 263
Cdd:cd05914 84 FVSDE-------------------------------------------------------------------DDVALINY 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 264 TSGSTGKPKGVVHTTGGYLVYAAMTFKYVFdYHPGD----------IYWCTADV---GWVTGHSYLLYGPLACGAMTLMF 330
Cdd:cd05914 97 TSGTTGNSKGVMLTYRNIVSNVDGVKEVVL-LGKGDkilsilplhhIYPLTFTLllpLLNGAHVVFLDKIPSAKIIALAF 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 331 EGVPNYPGVNRLGQVIDKHQVNI------------LYTAPTAIRALMAEGDKAIEGTKrTSLRIMGSVGEPINPEAWEWY 398
Cdd:cd05914 176 AQVTPTLGVPVPLVIEKIFKMDIipkltlkkfkfkLAKKINNRKIRKLAFKKVHEAFG-GNIKEFVIGGAKINPDVEEFL 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 399 YnKIGngqCPIVDTWWQTETGGFMITPLPGatELKAGSATRPFFGVQPALVDNlgNPQEGASE----GNLVITDSWPGQA 474
Cdd:cd05914 255 R-TIG---FPYTIGYGMTETAPIISYSPPN--RIRLGSAGKVIDGVEVRIDSP--DPATGEGEiivrGPNVMKGYYKNPE 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 475 RTlygdhdrfeQTYFsTFKGMYFSGDGARRDEDGYYWITGRVDDV-LNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHN 553
Cdd:cd05914 327 AT---------AEAF-DKDGWFHTGDLGKIDAEGYLYIRGRKKEMiVLSSGKNIYPEEIEAKINNMPFVLESLVVVQEKK 396
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 554 IKGQAIYAYITLNHGEEPTPELYTEVRNWVRKEIGpIATPD-------ILHWTDsLPKTRSGKIMR 612
Cdd:cd05914 397 LVALAYIDPDFLDVKALKQRNIIDAIKWEVRDKVN-QKVPNykkiskvKIVKEE-FEKTPKGKIKR 460
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
107-615 |
1.62e-20 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 96.65 E-value: 1.62e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 107 HVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITA 186
Cdd:PRK10252 483 QFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITT 562
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 187 DEgmRAGR--AIPlkknVDDALKNPAitsinnvvvfqrtgktgywqegrdLWWhdlivgVSADCPAEEMNAEDPLFILYT 264
Cdd:PRK10252 563 AD--QLPRfaDVP----DLTSLCYNA------------------------PLA------PQGAAPLQLSQPHHTAYIIFT 606
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 265 SGSTGKPKGVV--HTTggyLVYAAMTFKYVFDYHPGDIYW----CTADVG-WVtghsylLYGPLACGAMTLMFEgvpnyP 337
Cdd:PRK10252 607 SGSTGRPKGVMvgQTA---IVNRLLWMQNHYPLTADDVVLqktpCSFDVSvWE------FFWPFIAGAKLVMAE-----P 672
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 338 GVNR----LGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPE---AWE-WYYNKIGNGQCPI 409
Cdd:PRK10252 673 EAHRdplaMQQFFAEYGVTTTHFVPSMLAAFVASLTPEGARQSCASLRQVFCSGEALPADlcrEWQqLTGAPLHNLYGPT 752
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 410 ---VD-TWWqtetggfmitPLPGATELKAGSAT----RPFFGVQPALVDNLGNPQEGASEGNLVITdswpG-Q-ARTLYG 479
Cdd:PRK10252 753 eaaVDvSWY----------PAFGEELAAVRGSSvpigYPVWNTGLRILDARMRPVPPGVAGDLYLT----GiQlAQGYLG 818
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 480 DHD----RFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVV------G 549
Cdd:PRK10252 819 RPDltasRFIADPFAPGERMYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQALPDVEQAVTHacvinqA 898
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 550 VPHNIKGQAIYAYITLNHGEEPTPELyteVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:PRK10252 899 AATGGDARQLVGYLVSQSGLPLDTSA---LQAQLRERLPPHMVPVVLLQLDQLPLSANGKLDRKAL 961
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
91-615 |
3.06e-20 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 93.77 E-value: 3.06e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWEGddpsQSkhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDA 170
Cdd:cd17645 13 DHVAVVDRG----QS--LTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYPGER 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 171 VAGRIIDSNAKLVITadegmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpa 250
Cdd:cd17645 87 IAYMLADSSAKILLT----------------------------------------------------------------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 251 eemNAEDPLFILYTSGSTGKPKGVV--HTTggyLVYAAMTFKYVFDYHPGDIYWCTADVGWvTGHSYLLYGPLACGAMTL 328
Cdd:cd17645 102 ---NPDDLAYVIYTSGSTGLPKGVMieHHN---LVNLCEWHRPYFGVTPADKSLVYASFSF-DASAWEIFPHLTAGAALH 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 329 MFEGVPNYpGVNRLGQVIDKHQVNILYTaPTAIralmAEGDKAIEGTkrtSLRIMGSVGEPINPEAWEWY--YNKIGNGQ 406
Cdd:cd17645 175 VVPSERRL-DLDALNDYFNQEGITISFL-PTGA----AEQFMQLDNQ---SLRVLLTGGDKLKKIERKGYklVNNYGPTE 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 407 CPIVDTwwqtetgGFMITPLPGATEL-KAGSATRPFFgvqpaLVDNLGNPQEGASeGNLVITDSwpGQARTLYGDHD--- 482
Cdd:cd17645 246 NTVVAT-------SFEIDKPYANIPIgKPIDNTRVYI-----LDEALQLQPIGVA-GELCIAGE--GLARGYLNRPElta 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 483 -RFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYA 561
Cdd:cd17645 311 eKFIVHPFVPGERMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGRKYLVA 390
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 1206237686 562 YITLNhgEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd17645 391 YVTAP--EEIPHE---ELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
93-620 |
3.25e-20 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 94.56 E-value: 3.25e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 93 TAIIWEGDDPSQS---KHVTYKQLHHNVCQFAN-VLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSP 168
Cdd:PRK08751 33 TSVAKFADRPAYHsfgKTITYREADQLVEQFAAyLLGELQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTP 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 169 DAVAGRIIDSNAKLVITADE-GMRAGRAI---PLKK----NVDDALKNPAITSINNVVVFQRTGKTGYWQEGRDLWWHDL 240
Cdd:PRK08751 113 RELKHQLIDSGASVLVVIDNfGTTVQQVIadtPVKQvittGLGDMLGFPKAALVNFVVKYVKKLVPEYRINGAIRFREAL 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 241 IVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGV-----------------VHTTGGYLVYAAMTFKYVFDYHpgdIYWC 303
Cdd:PRK08751 193 ALGRKHSMPTLQIEPDDIAFLQYTGGTTGVAKGAmlthrnlvanmqqahqwLAGTGKLEEGCEVVITALPLYH---IFAL 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 304 TADvGWV----TGHSYLLYGP---------------LACGAMTLMFEGVPNYPGVNRlgqvIDKHQVNILYTAPTAIRAL 364
Cdd:PRK08751 270 TAN-GLVfmkiGGCNHLISNPrdmpgfvkelkktrfTAFTGVNTLFNGLLNTPGFDQ----IDFSSLKMTLGGGMAVQRS 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 365 MAEGDKAIEGTkrtslrimgsvgepinpeawewyynkigngqcPIVDTWWQTETG-GFMITPLpgatELKA--GSATRPF 441
Cdd:PRK08751 345 VAERWKQVTGL--------------------------------TLVEAYGLTETSpAACINPL----TLKEynGSIGLPI 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 442 FGVQPALVDNLGNPQEGASEGNLVITDSwpgqaRTLYGDHDRFEQT-YFSTFKGMYFSGDGARRDEDGYYWITGRVDDVL 520
Cdd:PRK08751 389 PSTDACIKDDAGTVLAIGEIGELCIKGP-----QVMKGYWKRPEETaKVMDADGWLHTGDIARMDEQGFVYIVDRKKDMI 463
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 521 NVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITlnhgeEPTPELYTE-VRNWVRKEIGPIATPDILHWT 599
Cdd:PRK08751 464 LVSGFNVYPNEIEDVIAMMPGVLEVAAVGVPDEKSGEIVKVVIV-----KKDPALTAEdVKAHARANLTGYKQPRIIEFR 538
|
570 580
....*....|....*....|.
gi 1206237686 600 DSLPKTRSGKIMRRILRKIAA 620
Cdd:PRK08751 539 KELPKTNVGKILRRELRDAAK 559
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
109-616 |
2.43e-19 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 91.48 E-value: 2.43e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADE 188
Cdd:PRK07514 30 TYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAELDYFIGDAEPALVVCDPA 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 189 GMRAGRAIPLKKNVddalknPAITSINNvvvfQRTGKTGywqegrdlwwhDLIVGVSADCPAEEMNAEDPLFILYTSGST 268
Cdd:PRK07514 110 NFAWLSKIAAAAGA------PHVETLDA----DGTGSLL-----------EAAAAAPDDFETVPRGADDLAAILYTSGTT 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 269 GKPKGVVHTTGGyLVYAAMTFKYVFDYHPGD-------IYwctadvgwvtgHSYLLY----GPLACGA-MTLM--FEgvp 334
Cdd:PRK07514 169 GRSKGAMLSHGN-LLSNALTLVDYWRFTPDDvlihalpIF-----------HTHGLFvatnVALLAGAsMIFLpkFD--- 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 335 nypgvnrLGQVIDK-HQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVgePINPEAWEWYYNKIGNgqcPIVDTW 413
Cdd:PRK07514 234 -------PDAVLALmPRATVMMGVPTFYTRLLQEPRLTREAAAHMRLFISGSA--PLLAETHREFQERTGH---AILERY 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 414 WQTETGgfMITPLPGATELKAGSATRPFFGVQPALVDnlgnPQEGASE-----GNL------VITDSW--PGQARTLYgd 480
Cdd:PRK07514 302 GMTETN--MNTSNPYDGERRAGTVGFPLPGVSLRVTD----PETGAELppgeiGMIevkgpnVFKGYWrmPEKTAEEF-- 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 481 hdrfeqtyfsTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIY 560
Cdd:PRK07514 374 ----------RADGFFITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIGVPHPDFGEGVT 443
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 561 AYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK07514 444 AVVVPKPGAALDEA---AILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLLR 496
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
70-619 |
2.60e-19 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 91.93 E-value: 2.60e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 70 FEDGTLNLSANC--------LDRHLTERGEQTAIIWEGddpsqsKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPM 141
Cdd:PRK08162 4 YEQGLDRNAANYvpltplsfLERAAEVYPDRPAVIHGD------RRRTWAETYARCRRLASALARRGIGRGDTVAVLLPN 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 142 VPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADEGMR-AGRAI-------PLKKNVDDALKNPAits 213
Cdd:PRK08162 78 IPAMVEAHFGVPMAGAVLNTLNTRLDAASIAFMLRHGEAKVLIVDTEFAEvAREALallpgpkPLVIDVDDPEYPGG--- 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 214 innvvvfQRTGKTGY---WQEGR-DLWWHdlivgvsadCPAEEMNAedpLFILYTSGSTGKPKGVV-HTTGGYLVYAAMT 288
Cdd:PRK08162 155 -------RFIGALDYeafLASGDpDFAWT---------LPADEWDA---IALNYTSGTTGNPKGVVyHHRGAYLNALSNI 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 289 FKYVFDYHPgdIY-----------WC-----TADVG------WVTghSYLLYGPLA-------CGA---MTLMFegvpNY 336
Cdd:PRK08162 216 LAWGMPKHP--VYlwtlpmfhcngWCfpwtvAARAGtnvclrKVD--PKLIFDLIRehgvthyCGApivLSALI----NA 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 337 PGVNRLGqvIDkHQVNILYTA---PTAIralmaegdkaIEGTKRTSLRIMGSVG--EPINPEAwewyynkigngQCPivd 411
Cdd:PRK08162 288 PAEWRAG--ID-HPVHAMVAGaapPAAV----------IAKMEEIGFDLTHVYGltETYGPAT-----------VCA--- 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 412 twWQTETGGFmitPLPGATELKAGSATRpfFGVQPALvdNLGNPQEGAS-------------EGNLVITDSWPGQARTly 478
Cdd:PRK08162 341 --WQPEWDAL---PLDERAQLKARQGVR--YPLQEGV--TVLDPDTMQPvpadgetigeimfRGNIVMKGYLKNPKAT-- 409
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 479 gdHDRFEQTYFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQA 558
Cdd:PRK08162 410 --EEAFAGGWFHT-------GDLAVLHPDGYIKIKDRSKDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEV 480
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 559 IYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTdSLPKTRSGKIMRRILRKIA 619
Cdd:PRK08162 481 PCAFVELKDGASATEE---EIIAHCREHLAGFKVPKAVVFG-ELPKTSTGKIQKFVLREQA 537
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
82-615 |
6.11e-19 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 92.15 E-value: 6.11e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEGDDpsqskhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSV 161
Cdd:PRK05691 1137 LNEQARQTPERIALVWDGGS------LDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVP 1210
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSPDAVAGRIIDSNAKLVITAdegmragRAIPLKKNVDDALKNPAITSINnvvvfqrtgktgywqegRDLWwhdli 241
Cdd:PRK05691 1211 LDPDYPAERLAYMLADSGVELLLTQ-------SHLLERLPQAEGVSAIALDSLH-----------------LDSW----- 1261
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 242 vgvSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAA-MTFKYVFDyhPGDIYWCTADVGWVTGhSYLLYGP 320
Cdd:PRK05691 1262 ---PSQAPGLHLHGDNLAYVIYTSGSTGQPKGVGNTHAALAERLQwMQATYALD--DSDVLMQKAPISFDVS-VWECFWP 1335
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 321 LACGAmTLMFEGVPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAiegtKRTSLRIMGSVGEPINPEAWE---- 396
Cdd:PRK05691 1336 LITGC-RLVLAGPGEHRDPQRIAELVQQYGVTTLHFVPPLLQLFIDEPLAA----ACTSLRRLFSGGEALPAELRNrvlq 1410
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 397 -----WYYNKIGNGQCPIVDTWWQTETGGFMITPLpgatelkagsaTRPFFGVQPALVDNLGNPQEGASEGNLVITDSwp 471
Cdd:PRK05691 1411 rlpqvQLHNRYGPTETAINVTHWQCQAEDGERSPI-----------GRPLGNVLCRVLDAELNLLPPGVAGELCIGGA-- 1477
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 472 GQARTLYG----DHDRF-EQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAA 546
Cdd:PRK05691 1478 GLARGYLGrpalTAERFvPDPLGEDGARLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEPEEIQARLLAQPGVAQAA 1557
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 547 VVgVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:PRK05691 1558 VL-VREGAAGAQLVGYYTGEAGQEAEAE---RLKAALAAELPEYMVPAQLIRLDQMPLGPSGKLDRRAL 1622
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
104-617 |
8.55e-19 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 89.80 E-value: 8.55e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 104 QSKHVTYKQLHHNVCQFANVLKK-LGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKL 182
Cdd:cd05937 2 EGKTWTYSETYDLVLRYAHWLHDdLGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPLIHCLKLSGSRF 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 183 VItadegmragraiplkknVDDalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemnaEDPLFIL 262
Cdd:cd05937 82 VI-----------------VDP---------------------------------------------------DDPAILI 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 263 YTSGSTGKPKGVVHTTGGYLVyAAMTFKYVFDYHPGD-IYWCTAdvgwvtghsyLLYGPLAC-GAMTLMFEGV-----PN 335
Cdd:cd05937 94 YTSGTTGLPKAAAISWRRTLV-TSNLLSHDLNLKNGDrTYTCMP----------LYHGTAAFlGACNCLMSGGtlalsRK 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 336 YPGVNRLGQVIDKHQVNILYTAPTaIRALMAeGDKAIEgTKRTSLRIMgsVGEPINPEAWEWYYNKIGngqCPIVD---- 411
Cdd:cd05937 163 FSASQFWKDVRDSGATIIQYVGEL-CRYLLS-TPPSPY-DRDHKVRVA--WGNGLRPDIWERFRERFN---VPEIGefya 234
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 412 ------TWWQTETGGFMItplpGATELKaGSATRPFFGVQPALV-------DNLGNPQEG-------ASEGNLVIT---- 467
Cdd:cd05937 235 ategvfALTNHNVGDFGA----GAIGHH-GLIRRWKFENQVVLVkmdpetdDPIRDPKTGfcvrapvGEPGEMLGRvpfk 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 468 --DSWPG--------QARTLygdHDRFEqtyfstfKG-MYF-SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESA 535
Cdd:cd05937 310 nrEAFQGylhnedatESKLV---RDVFR-------KGdIYFrTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADV 379
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 536 LVSHPKIAEAAVVGV--PHNiKGQAIYAYITL-NHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMR 612
Cdd:cd05937 380 LGAHPDIAEANVYGVkvPGH-DGRAGCAAITLeESSAVPTEFTKSLLASLARKNLPSYAVPLFLRLTEEVATTDNHKQQK 458
|
....*
gi 1206237686 613 RILRK 617
Cdd:cd05937 459 GVLRD 463
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
260-615 |
1.70e-18 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 90.61 E-value: 1.70e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 260 FILYTSGSTGKPKGVVHTTGGYlvyaAMTFKYV---FDYHPGD----IYWCTADVGwvtghSYLLYGPLACGA-MTLMFE 331
Cdd:PRK05691 2337 YLIYTSGSTGKPKGVVVSHGEI----AMHCQAVierFGMRADDcelhFYSINFDAA-----SERLLVPLLCGArVVLRAQ 2407
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 332 GvpnYPGVNRLGQVIDKHQVNILYTAPT----AIRALMAEGDkaiegtkRTSLRIMGSVGEPINPEAW---------EWY 398
Cdd:PRK05691 2408 G---QWGAEEICQLIREQQVSILGFTPSygsqLAQWLAGQGE-------QLPVRMCITGGEALTGEHLqrirqafapQLF 2477
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 399 YNKIGngqcpivdtwwQTETggfMITPL--PGATELKAGSATRPFFGVQPALV-----DNLGNPQEGASeGNLVItdswp 471
Cdd:PRK05691 2478 FNAYG-----------PTET---VVMPLacLAPEQLEEGAASVPIGRVVGARVayildADLALVPQGAT-GELYV----- 2537
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 472 GQARTLYGDHDR-------FEQTYFSTFKG-MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIA 543
Cdd:PRK05691 2538 GGAGLAQGYHDRpgltaerFVADPFAADGGrLYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVR 2617
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1206237686 544 EAAVVGVpHNIKGQAIYAYI---TLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:PRK05691 2618 EAVVLAL-DTPSGKQLAGYLvsaVAGQDDEAQAALREALKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRAL 2691
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
233-642 |
2.59e-18 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 90.23 E-value: 2.59e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 233 RDLWWHDLIVGVSADCPAEEMNAEDPL-FILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVfDYHPGDIYWCTADVGWVT 311
Cdd:PRK05691 3845 RLLVWEEVQAGEVASHNPGIYSGPDNLaYVIYTSGSTGLPKGVMVEQRGMLNNQLSKVPYL-ALSEADVIAQTASQSFDI 3923
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 312 GHSYLLYGPLACGAMTLMFEGVPNYPGvNRLGQVIDKhQVNILYTAPTAIRALMAEGDKAIEGtkrtsLRIMGSVGEPIN 391
Cdd:PRK05691 3924 SVWQFLAAPLFGARVEIVPNAIAHDPQ-GLLAHVQAQ-GITVLESVPSLIQGMLAEDRQALDG-----LRWMLPTGEAMP 3996
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 392 PE-AWEWY--YNKIGngqcpIVDTWWQTETG---GFMITPLPG--ATELKAGSATrpffgvqpalvDN-----LGNPQE- 457
Cdd:PRK05691 3997 PElARQWLqrYPQIG-----LVNAYGPAECSddvAFFRVDLAStrGSYLPIGSPT-----------DNnrlylLDEALEl 4060
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 458 ---GASeGNLVITDSwpGQARTLYGDHDRFEQTYFSTFKG-----MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGT 529
Cdd:PRK05691 4061 vplGAV-GELCVAGT--GVGRGYVGDPLRTALAFVPHPFGapgerLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIEL 4137
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 530 AEIESALVSHPKIAEAAvVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGK 609
Cdd:PRK05691 4138 GEIEARLHEQAEVREAA-VAVQEGVNGKHLVGYLVPHQTVLAQGALLERIKQRLRAELPDYMVPLHWLWLDRLPLNANGK 4216
|
410 420 430
....*....|....*....|....*....|...
gi 1206237686 610 IMRRILrkiAAGDTSNLGDTSTLADPSVVEKLL 642
Cdd:PRK05691 4217 LDRKAL---PALDIGQLQSQAYLAPRNELEQTL 4246
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
261-612 |
2.67e-18 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 86.40 E-value: 2.67e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 261 ILYTSGSTGKPKGVV--HTTGgYLVYAAmtfkyvfdyhpgdiyWCtaDVGWVT-GHSYLLYGPL------ACGAMTLMFE 331
Cdd:cd17638 5 IMFTSGTTGRSKGVMcaHRQT-LRAAAA---------------WA--DCADLTeDDRYLIINPFfhtfgyKAGIVACLLT 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 332 GVPNYP----GVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKaiEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNGQc 407
Cdd:cd17638 67 GATVVPvavfDVDAILEAIERERITVLPGPPTLFQSLLDHPGR--KKFDLSSLRAAVTGAATVPVELVRRMRSELGFET- 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 408 pIVDTWWQTETG-GFMITPLPGATELkAGSATRPFFGVQPALVDnlgnPQEGASEGNLVIT---DSWPGQARTLYGDhdr 483
Cdd:cd17638 144 -VLTAYGLTEAGvATMCRPGDDAETV-ATTCGRACPGFEVRIAD----DGEVLVRGYNVMQgylDDPEATAEAIDAD--- 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 484 feqtyfstfkGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYI 563
Cdd:cd17638 215 ----------GWLHTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFV 284
|
330 340 350 360
....*....|....*....|....*....|....*....|....*....
gi 1206237686 564 TLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMR 612
Cdd:cd17638 285 VARPGVTLTEE---DVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
84-617 |
1.27e-17 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 86.52 E-value: 1.27e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 84 RHLTERGEQTAIIWEGDDPSQSKHVTYKQLHHNVCQFANVLKKLGvKKGDVVAIYMPMVPEAAVAMLACARIGAVhSVIF 163
Cdd:cd05931 1 RRAAARPDRPAYTFLDDEGGREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAI-AVPL 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 164 ggFSPDA------VAGRIIDSNAKLVITADEgmragraiplkknVDDALKnpaitsinnvvvfqRTGKTGYWQEGRDLWW 237
Cdd:cd05931 79 --PPPTPgrhaerLAAILADAGPRVVLTTAA-------------ALAAVR--------------AFAASRPAAGTPRLLV 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 238 HDLI-VGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKyVFDYHPGDIY--W--CTADVGWVTG 312
Cdd:cd05931 130 VDLLpDTSAADWPPPSPDPDDIAYLQYTSGSTGTPKGVVVTHRNLLANVRQIRR-AYGLDPGDVVvsWlpLYHDMGLIGG 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 313 hsylLYGPLACGA-MTLM----FegvpnypgVNR----LgQVIDKHQVNILYtAPT-----AIRALMAEGdkaIEGTKRT 378
Cdd:cd05931 209 ----LLTPLYSGGpSVLMspaaF--------LRRplrwL-RLISRYRATISA-APNfaydlCVRRVRDED---LEGLDLS 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 379 SLRIMGSVGEPINPEAWEWYYNK--------------------------IGNGQCPIVDTW--WQTETGGFMITPL-PGA 429
Cdd:cd05931 272 SWRVALNGAEPVRPATLRRFAEAfapfgfrpeafrpsyglaeatlfvsgGPPGTGPVVLRVdrDALAGRAVAVAADdPAA 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 430 TELKagSATRPFFGVQPALVDnlgnPQEGASEGNLVITDSW---PGQARTLYGDHDRFEQTYF---STFKGMYF-SGD-G 501
Cdd:cd05931 352 RELV--SCGRPLPDQEVRIVD----PETGRELPDGEVGEIWvrgPSVASGYWGRPEATAETFGalaATDEGGWLrTGDlG 425
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 502 ARRdeDGYYWITGRVDDVLNVSGHRLGTAEIE-SALVSHPKIAE--AAVVGVPHNIKGQ-AIYAYITLNHGEEPTPELYT 577
Cdd:cd05931 426 FLH--DGELYITGRLKDLIIVRGRNHYPQDIEaTAEEAHPALRPgcVAAFSVPDDGEERlVVVAEVERGADPADLAAIAA 503
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 1206237686 578 EVRNWVRKEIGpIATPDI-LHWTDSLPKTRSGKIMRRILRK 617
Cdd:cd05931 504 AIRAAVAREHG-VAPADVvLVRPGSIPRTSSGKIQRRACRA 543
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
109-616 |
3.15e-17 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 85.49 E-value: 3.15e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLK-KLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAK-LVITA 186
Cdd:PRK08974 50 TFRKLEERSRAFAAYLQnGLGLKKGDRVALMMPNLLQYPIALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAKaIVIVS 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 187 DegmragRAIPLKKNVD-------------DALKNPAITSINNVVVFQRTGKTGY-----------WQEGRDLWWhdliv 242
Cdd:PRK08974 130 N------FAHTLEKVVFktpvkhviltrmgDQLSTAKGTLVNFVVKYIKRLVPKYhlpdaisfrsaLHKGRRMQY----- 198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 243 gVSADcpaeeMNAEDPLFILYTSGSTGKPKGVVHTTGGYLvyaamtfkyvfdyhpgdiywctADVGWVTGhsylLYGPLA 322
Cdd:PRK08974 199 -VKPE-----LVPEDLAFLQYTGGTTGVAKGAMLTHRNML----------------------ANLEQAKA----AYGPLL 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 323 CGAMTLMFEGVPNYP----GVNRLgQVIDKHQVNILYTAPTAIRALMAEGDK----AIEGTKR----------------T 378
Cdd:PRK08974 247 HPGKELVVTALPLYHifalTVNCL-LFIELGGQNLLITNPRDIPGFVKELKKypftAITGVNTlfnallnneefqeldfS 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 379 SLRImgSVGepinpeawewyynkignGQCPI---VDTWWQTETG-----GFMIT---PL----PGATELKAGSATRPFFG 443
Cdd:PRK08974 326 SLKL--SVG-----------------GGMAVqqaVAERWVKLTGqylleGYGLTecsPLvsvnPYDLDYYSGSIGLPVPS 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 444 VQPALVDNLGNPQEGASEGNLvitdsWPGQARTLYGDHDRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVS 523
Cdd:PRK08974 387 TEIKLVDDDGNEVPPGEPGEL-----WVKGPQVMLGYWQRPEATDEVIKDGWLATGDIAVMDEEGFLRIVDRKKDMILVS 461
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 524 GHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHgEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLP 603
Cdd:PRK08974 462 GFNVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFVVKKD-PSLTEE---ELITHCRRHLTGYKVPKLVEFRDELP 537
|
570
....*....|...
gi 1206237686 604 KTRSGKIMRRILR 616
Cdd:PRK08974 538 KSNVGKILRRELR 550
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
480-617 |
9.60e-17 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 82.40 E-value: 9.60e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 480 DHDRF-EQTYFSTfkgmyfsgDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQA 558
Cdd:PRK07824 226 DPDPFaEPGWFRT--------DDLGALDDGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQR 297
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 559 IYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:PRK07824 298 VVAAVVGDGGPAPTLE---ALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALVR 353
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
100-617 |
1.32e-16 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 82.74 E-value: 1.32e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 100 DDPSQSkhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSN 179
Cdd:cd17653 17 ESLGGS--LTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAKLPSARIQAILRTSG 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 180 AKLVITADegmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemNAEDPL 259
Cdd:cd17653 95 ATLLLTTD------------------------------------------------------------------SPDDLA 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 260 FILYTSGSTGKPKGVVHTTGGYLVYA---------------AMTFKYVFDYHPGDIYwctadvgwvtghSYLLYGPlacg 324
Cdd:cd17653 109 YIIFTSGSTGIPKGVMVPHRGVLNYVsqpparldvgpgsrvAQVLSIAFDACIGEIF------------STLCNGG---- 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 325 amTLMFEGVPNYpgvnrLGQVIDKhqVNILYTAPTAIRALMAEGdkaiegtkRTSLRIMGSVGEPINP---EAWEW---Y 398
Cdd:cd17653 173 --TLVLADPSDP-----FAHVART--VDALMSTPSILSTLSPQD--------FPNLKTIFLGGEAVPPsllDRWSPgrrL 235
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 399 YNKIGNGQCPIVDTWWQTETGGFMI--TPLPGATELkagsatrpffgvqpaLVDNLGNPQEGASEGNLVItdSWPGQART 476
Cdd:cd17653 236 YNAYGPTECTISSTMTELLPGQPVTigKPIPNSTCY---------------ILDADLQPVPEGVVGEICI--SGVQVARG 298
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 477 LYGDH----DRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVpH 552
Cdd:cd17653 299 YLGNPaltaSKFVPDPFWPGSRMYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVLQSQPEVTQAAAIV-V 377
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1206237686 553 NikgQAIYAYItlnhgeepTPELYTE--VRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:cd17653 378 N---GRLVAFV--------TPETVDVdgLRSELAKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
247-615 |
1.59e-16 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 82.74 E-value: 1.59e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 247 DCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGgylvyaamtfkyvfdyhpgdiywCTADVG-WVT--GHSYLLYGPLAC 323
Cdd:PRK13383 165 ESGGRPAVAAPGRIVLLTSGTTGKPKGVPRAPQ-----------------------LRSAVGvWVTilDRTRLRTGSRIS 221
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 324 GAMTlMFEGVpnypGVNRL-------GQVIDK--------------HQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRI 382
Cdd:PRK13383 222 VAMP-MFHGL----GLGMLmltialgGTVLTHrhfdaeaalaqaslHRADAFTAVPVVLARILELPPRVRARNPLPQLRV 296
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 383 MGSVGEPINPEAWEWYYNKIGNgqcPIVDTWWQTETG-GFMITPlpgaTELKAGSAT--RPFFGVQPALVDNLGNPQEGA 459
Cdd:PRK13383 297 VMSSGDRLDPTLGQRFMDTYGD---ILYNGYGSTEVGiGALATP----ADLRDAPETvgKPVAGCPVRILDRNNRPVGPR 369
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 460 SEGNLVITDSWPGQARTLYGDHdrfeqtyfSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSH 539
Cdd:PRK13383 370 VTGRIFVGGELAGTRYTDGGGK--------AVVDGMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAH 441
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 540 PKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:PRK13383 442 PAVADNAVIGVPDERFGHRLAAFVVLHPGSGVDAA---QLRDYLKDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
108-549 |
3.63e-16 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 82.09 E-value: 3.63e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITAD 187
Cdd:cd17641 12 FTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIAED 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 EgmragraiplkKNVDDALK-NPAITSINNVVVFQRTGKTGYwQEGRDLWWHDLI-VGVSADC--PAE------EMNAED 257
Cdd:cd17641 92 E-----------EQVDKLLEiADRIPSVRYVIYCDPRGMRKY-DDPRLISFEDVVaLGRALDRrdPGLyerevaAGKGED 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 258 PLFILYTSGSTGKPKGVVHTTGGYL----VYAAMTFKyvfdyHPGDIYWCTADVGWVTGHSYLLYGPLACG--------- 324
Cdd:cd17641 160 VAVLCTTSGTTGKPKLAMLSHGNFLghcaAYLAADPL-----GPGDEYVSVLPLPWIGEQMYSVGQALVCGfivnfpeep 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 325 --AMTLMFEGVPNY----PGV---------------NRLGQVIDKHQVNILYTA----------PTAIRALMAEGDKAIE 373
Cdd:cd17641 235 etMMEDLREIGPTFvllpPRVwegiaadvrarmmdaTPFKRFMFELGMKLGLRAldrgkrgrpvSLWLRLASWLADALLF 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 374 GTKR-----TSLRIMGSVGEPINPEAWEwYYNKIGngqCPIVDTWWQTETGGFmITPLPGAtELKAGSATRPFFGVQpAL 448
Cdd:cd17641 315 RPLRdrlgfSRLRSAATGGAALGPDTFR-FFHAIG---VPLKQLYGQTELAGA-YTVHRDG-DVDPDTVGVPFPGTE-VR 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 449 VDNLGnpqEGASEGNLVITDSWPGQARTlygDHDRFEQTYFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVS-GHRL 527
Cdd:cd17641 388 IDEVG---EILVRSPGVFVGYYKNPEAT---AEDFDEDGWLHT-------GDAGYFKENGHLVVIDRAKDVGTTSdGTRF 454
|
490 500
....*....|....*....|..
gi 1206237686 528 GTAEIESALVSHPKIAEAAVVG 549
Cdd:cd17641 455 SPQFIENKLKFSPYIAEAVVLG 476
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
82-620 |
7.05e-16 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 80.80 E-value: 7.05e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLteRGEQTAIIwegddpSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVhsV 161
Cdd:PRK10946 31 LTRHA--ASDAIAVI------CGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVA--P 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 162 IFGGFSP-----DAVAGRIidsNAKLVItadegmrAGRAIPLKKNVD--DALKNpAITSINNVVVFQRTGktgywqeGRD 234
Cdd:PRK10946 101 VNALFSHqrselNAYASQI---EPALLI-------ADRQHALFSDDDflNTLVA-EHSSLRVVLLLNDDG-------EHS 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 235 LwwHDLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLvYAAMTFKYVFDYHPGDIYWCTADvgwvTGHS 314
Cdd:PRK10946 163 L--DDAINHPAEDFTATPSPADEVAFFQLSGGSTGTPKLIPRTHNDYY-YSVRRSVEICGFTPQTRYLCALP----AAHN 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 315 YLLYGPlacGAMTLMFEG-----VPNyPGVNRLGQVIDKHQVNILYTAPTA----IRALMAEGDKAiegtKRTSLRIM-- 383
Cdd:PRK10946 236 YPMSSP---GALGVFLAGgtvvlAPD-PSATLCFPLIEKHQVNVTALVPPAvslwLQAIAEGGSRA----QLASLKLLqv 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 384 ------GSVGEPINPEawewyynkIGngqCPIVDTWWQTEtGGFMITPLPGATELKAGSATRPffgVQPA----LVDNLG 453
Cdd:PRK10946 308 ggarlsETLARRIPAE--------LG---CQLQQVFGMAE-GLVNYTRLDDSDERIFTTQGRP---MSPDdevwVADADG 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 454 NPQEGASEGNLVITDswPGQARTLYG--DHDRfeqtyfSTF--KGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGT 529
Cdd:PRK10946 373 NPLPQGEVGRLMTRG--PYTFRGYYKspQHNA------SAFdaNGFYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAA 444
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 530 AEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPtpelyTEVRNWVRkEIGpIAT---PDILHWTDSLPKTR 606
Cdd:PRK10946 445 EEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVKEPLKA-----VQLRRFLR-EQG-IAEfklPDRVECVDSLPLTA 517
|
570
....*....|....
gi 1206237686 607 SGKIMRRILRKIAA 620
Cdd:PRK10946 518 VGKVDKKQLRQWLA 531
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
498-616 |
1.74e-15 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 79.81 E-value: 1.74e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 498 SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPElyt 577
Cdd:PRK05677 437 TGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAALPGVLQCAAIGVPDEKSGEAIKVFVVVKPGETLTKE--- 513
|
90 100 110
....*....|....*....|....*....|....*....
gi 1206237686 578 EVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK05677 514 QVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELR 552
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
254-615 |
3.29e-15 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 78.63 E-value: 3.29e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 254 NAEDPLFILYTSGSTGKPKGVV--------HTTGGYLVYAAMTFKYV-------FDYHPGDIYwctadVGWVTGHSYLLy 318
Cdd:cd17644 104 QPENLAYVIYTSGSTGKPKGVMiehqslvnLSHGLIKEYGITSSDRVlqfasiaFDVAAEEIY-----VTLLSGATLVL- 177
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 319 gplacgamtlmfegVPN--YPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKA-IEGTKRTSLRIMGsvGEPINPEAW 395
Cdd:cd17644 178 --------------RPEemRSSLEDFVQYIQQWQLTVLSLPPAYWHLLVLELLLStIDLPSSLRLVIVG--GEAVQPELV 241
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 396 E-W---------YYNKIGNGQCPIVDTWwqtetggFMITPLPGATELKAgSATRPFFGVQPALVDNLGNPQEGASEGNLV 465
Cdd:cd17644 242 RqWqknvgnfiqLINVYGPTEATIAATV-------CRLTQLTERNITSV-PIGRPIANTQVYILDENLQPVPVGVPGELH 313
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 466 ItdswpGQARTLYGDHDRFEQT---------YFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESAL 536
Cdd:cd17644 314 I-----GGVGLARGYLNRPELTaekfishpfNSSESERLYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVL 388
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 537 VSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPelyTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:cd17644 389 SQHNDVKTAVVIVREDQPGNKRLVAYIVPHYEESPST---VELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
106-616 |
3.33e-15 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 78.91 E-value: 3.33e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 106 KHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVIT 185
Cdd:PRK07059 47 KAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIVV 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 186 ADEGMRAGRAIPLKKNVddalKNPAITS----------INNVVVFQRTGKTGYWQEGRDLWWHD-LIVGVSADCPAEEMN 254
Cdd:PRK07059 127 LENFATTVQQVLAKTAV----KHVVVASmgdllgfkghIVNFVVRRVKKMVPAWSLPGHVRFNDaLAEGARQTFKPVKLG 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 255 AEDPLFILYTSGSTGKPKGVVHTTGGYL---------VYAAMTFKYVFD----------YHpgdIYWCTAdvgwvtghSY 315
Cdd:PRK07059 203 PDDVAFLQYTGGTTGVSKGATLLHRNIVanvlqmeawLQPAFEKKPRPDqlnfvcalplYH---IFALTV--------CG 271
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 316 LLygPLACGAMTLMfegVPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMG--SVGEPInPE 393
Cdd:PRK07059 272 LL--GMRTGGRNIL---IPNPRDIPGFIKELKKYQVHIFPAVNTLYNALLNNPDFDKLDFSKLIVANGGgmAVQRPV-AE 345
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 394 AWewyYNKIGngqCPIVDTWWQTETGGFMITPLPGATELkAGSATRPFFGVQPALVDN------LGNPQEGASEGNLVIT 467
Cdd:PRK07059 346 RW---LEMTG---CPITEGYGLSETSPVATCNPVDATEF-SGTIGLPLPSTEVSIRDDdgndlpLGEPGEICIRGPQVMA 418
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 468 DSW--PGQ-ARTLYGDhdrfeqtyfstfkGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAE 544
Cdd:PRK07059 419 GYWnrPDEtAKVMTAD-------------GFFRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVASHPGVLE 485
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1206237686 545 AAVVGVPHNIKGQAIYAYITlnhgeEPTPELYTE-VRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK07059 486 VAAVGVPDEHSGEAVKLFVV-----KKDPALTEEdVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRRELR 553
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
82-619 |
9.33e-15 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 77.58 E-value: 9.33e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 82 LDRHLTERGEQTAIIWEgddpsqSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVP---EA------AVAMLAC 152
Cdd:PLN02479 26 LERAAVVHPTRKSVVHG------SVRYTWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPamyEAhfgvpmAGAVVNC 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 153 A--RIGAVHSVIFGGFSPDAV-------------AGRIIDSNAK-------LVITADEgmragRAIPlkKNVDDALKNPA 210
Cdd:PLN02479 100 VniRLNAPTIAFLLEHSKSEVvmvdqefftlaeeALKILAEKKKssfkpplLIVIGDP-----TCDP--KSLQYALGKGA 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 211 ITSINnvvvFQRTGKTGY-WQEGRDLWwhdlivgvsadcpaeemnaeDPLFILYTSGSTGKPKGVV-HTTGGYLvyAAMT 288
Cdd:PLN02479 173 IEYEK----FLETGDPEFaWKPPADEW--------------------QSIALGYTSGTTASPKGVVlHHRGAYL--MALS 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 289 FKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMfegvpNYPGVNRLGQVIDKHQVNILYTAPTAIRALmaeg 368
Cdd:PLN02479 227 NALIWGMNEGAVYLWTLPMFHCNGWCFTWTLAALCGTNICL-----RQVTAKAIYSAIANYGVTHFCAAPVVLNTI---- 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 369 dkaiegtkrtslrIMGSVGEPINPEAWEWYYNKIGNGQCPIVdtWWQTETGGFMITPLPGATELKAGS---ATRPFFGVQ 445
Cdd:PLN02479 298 -------------VNAPKSETILPLPRVVHVMTAGAAPPPSV--LFAMSEKGFRVTHTYGLSETYGPStvcAWKPEWDSL 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 446 PALVDNLGNPQEG----ASEG-NLVITDSW---PGQARTL-------------YGDHDRFEQTYFSTfkGMYFSGDGARR 504
Cdd:PLN02479 363 PPEEQARLNARQGvryiGLEGlDVVDTKTMkpvPADGKTMgeivmrgnmvmkgYLKNPKANEEAFAN--GWFHSGDLGVK 440
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 505 DEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPTPE--LYTEVRNW 582
Cdd:PLN02479 441 HPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESPCAFVTLKPGVDKSDEaaLAEDIMKF 520
|
570 580 590
....*....|....*....|....*....|....*..
gi 1206237686 583 VRKEIGPIATPDILHWtDSLPKTRSGKIMRRILRKIA 619
Cdd:PLN02479 521 CRERLPAYWVPKSVVF-GPLPKTATGKIQKHVLRAKA 556
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
407-619 |
1.45e-14 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 76.78 E-value: 1.45e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 407 CPIVDTWWQTETGGFMITPlPGATELKAGSATRPFFGVQPALVDNLGNPQEGASEGNLVITDSwpgqaRTLYGDHDRFEQ 486
Cdd:PRK12492 359 CTIVEGYGLTETSPVASTN-PYGELARLGTVGIPVPGTALKVIDDDGNELPLGERGELCIKGP-----QVMKGYWQQPEA 432
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 487 TYFS-TFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITl 565
Cdd:PRK12492 433 TAEAlDAEGWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVV- 511
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....
gi 1206237686 566 nhGEEPTPELyTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRKIA 619
Cdd:PRK12492 512 --ARDPGLSV-EELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRRELRDIA 562
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
106-617 |
1.49e-14 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 76.31 E-value: 1.49e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 106 KHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVIT 185
Cdd:cd05939 2 RHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALIF 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 186 adegmragraiplkkNVDDALKNPAITSINnvvvfqrtgktgywqegrdlwwhdlivgvSADCpaeeMNAEDPLFILYTS 265
Cdd:cd05939 82 ---------------NLLDPLLTQSSTEPP-----------------------------SQDD----VNFRDKLFYIYTS 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 266 GSTGKPKGVVHTTGGYLVYAAMTFkYVFDYHPGDIywctadvgwvtghsylLYGPLAcgamtlMFEGVPNYPGVnrlGQV 345
Cdd:cd05939 114 GTTGLPKAAVIVHSRYYRIAAGAY-YAFGMRPEDV----------------VYDCLP------LYHSAGGIMGV---GQA 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 346 I---------------------DKHQVNILYTAPTAIRALMAEgdKAIEGTKRTSLRIMgsVGEPINPEAWEWYYN---- 400
Cdd:cd05939 168 LlhgstvvirkkfsasnfwddcVKYNCTIVQYIGEICRYLLAQ--PPSEEEQKHNVRLA--VGNGLRPQIWEQFVRrfgi 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 401 -KIG------NGQCPIVDTWWQTETGGFMITPLPGatelkagsatrpFFGVQPALVDNLGNPQEGASEGNLVITDswPGQ 473
Cdd:cd05939 244 pQIGefygatEGNSSLVNIDNHVGACGFNSRILPS------------VYPIRLIKVDEDTGELIRDSDGLCIPCQ--PGE 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 474 ARTLYGDHD------RFE----------QTYFSTFKG---MYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIES 534
Cdd:cd05939 310 PGLLVGKIIqndplrRFDgyvnegatnkKIARDVFKKgdsAFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEG 389
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 535 ALVSHPKIAEAAVVGV--PHnIKGQAIYAYITLNHGEEPTPELYTEvrnwVRKEIGPIATPDILHWTDSLPKTRSGKIMR 612
Cdd:cd05939 390 ILSNVLGLEDVVVYGVevPG-VEGRAGMAAIVDPERKVDLDRFSAV----LAKSLPPYARPQFIRLLPEVDKTGTFKLQK 464
|
....*
gi 1206237686 613 RILRK 617
Cdd:cd05939 465 TDLQK 469
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
109-549 |
1.55e-14 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 76.74 E-value: 1.55e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVIT--- 185
Cdd:cd05932 8 TWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFVgkl 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 186 -ADEGMRAGRAIPLKKnvddalknpAITSINNVVVFQRTgktgywqegrdlwWHDLIvgvSADCPAEEMNAEDP---LFI 261
Cdd:cd05932 88 dDWKAMAPGVPEGLIS---------ISLPPPSAANCQYQ-------------WDDLI---AQHPPLEERPTRFPeqlATL 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 262 LYTSGSTGKPKGVVHTTGGYlVYAAMTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGAMTLMFEGVPNYP---- 337
Cdd:cd05932 143 IYTSGTTGQPKGVMLTFGSF-AWAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAFAESLDTFVedvq 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 338 --------GVNRL-----GQVIDK---HQVNILYTAPTaIRALMaeGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNk 401
Cdd:cd05932 222 rarptlffSVPRLwtkfqQGVQDKipqQKLNLLLKIPV-VNSLV--KRKVLKGLGLDQCRLAGCGSAPVPPALLEWYRS- 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 402 IGngqCPIVDTWWQTETGGFMITPLPGATelKAGSATRPFFGVQPALVDnlgnpqegasEGNLVITDswPGqarTLYGDH 481
Cdd:cd05932 298 LG---LNILEAYGMTENFAYSHLNYPGRD--KIGTVGNAGPGVEVRISE----------DGEILVRS--PA---LMMGYY 357
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 482 DRFEQTYFS-TFKGMYFSGDGARRDEDGYYWITGRVDDVLNVS-GHRLGTAEIESALVSHPKIAEAAVVG 549
Cdd:cd05932 358 KDPEATAEAfTADGFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIENKLAEHDRVEMVCVIG 427
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
106-549 |
6.19e-13 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 71.24 E-value: 6.19e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 106 KHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVhsvifggfspDAVAGRIIDSNAKLVIT 185
Cdd:cd17640 4 KRITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAV----------DVVRGSDSSVEELLYIL 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 186 ADEGMRAgraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdLIVgvsadcpaeEMNAEDPLFILYTS 265
Cdd:cd17640 74 NHSESVA-----------------------------------------------LVV---------ENDSDDLATIIYTS 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 266 GSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWCTADVgWvtgHSY---LLYGPLACGaMTLMFEGVPNYPgvnrl 342
Cdd:cd17640 98 GTTGNPKGVMLTHAN-LLHQIRSLSDIVPPQPGDRFLSILPI-W---HSYersAEYFIFACG-CSQAYTSIRTLK----- 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 343 gQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMG---SVGE---PIN-----PEAWEWYYNKIGngqCPIVD 411
Cdd:cd17640 167 -DDLKRVKPHYIVSVPRLWESLYSGIQKQVSKSSPIKQFLFLfflSGGIfkfGISgggalPPHVDTFFEAIG---IEVLN 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 412 TWWQTETGGFMITPLPGATelKAGSATRPFFGVQPALVDNLGN-PQEGASEGNLVITDswPGQARTLYGDHDRFEQTYFS 490
Cdd:cd17640 243 GYGLTETSPVVSARRLKCN--VRGSVGRPLPGTEIKIVDPEGNvVLPPGEKGIVWVRG--PQVMKGYYKNPEATSKVLDS 318
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1206237686 491 tfKGMYFSGDGARRDEDGYYWITGRVDD--VLNvSGHRLGTAEIESALVSHPKIAEAAVVG 549
Cdd:cd17640 319 --DGWFNTGDLGWLTCGGELVLTGRAKDtiVLS-NGENVEPQPIEEALMRSPFIEQIMVVG 376
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
263-616 |
2.00e-12 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 70.12 E-value: 2.00e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 263 YTSGSTGKPKGVV--HTTGGYLVYAAMTfkyvfdyhPgDIYWCTA-DV-----------GWVTGHSYLLYGplacgaMTL 328
Cdd:PRK07008 183 YTSGTTGNPKGALysHRSTVLHAYGAAL--------P-DAMGLSArDAvlpvvpmfhvnAWGLPYSAPLTG------AKL 247
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 329 MFEGvPNYPGVNrLGQVIDKHQVNILYTAPTAIRALMAEGDKAieGTKRTSLR---IMGSVGEPINPEAWEWYYNkigng 405
Cdd:PRK07008 248 VLPG-PDLDGKS-LYELIEAERVTFSAGVPTVWLGLLNHMREA--GLRFSTLRrtvIGGSACPPAMIRTFEDEYG----- 318
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 406 qCPIVDTWWQTEtggfmITPLPGATELKAGSATRP--------------FFGVQPALVDNLGN--PQEGASEGNLVITDS 469
Cdd:PRK07008 319 -VEVIHAWGMTE-----MSPLGTLCKLKWKHSQLPldeqrkllekqgrvIYGVDMKIVGDDGRelPWDGKAFGDLQVRGP 392
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 470 WPGQaRTLYGDHDRFEQTYFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVG 549
Cdd:PRK07008 393 WVID-RYFRGDASPLVDGWFPT-------GDVATIDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAEAACIA 464
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1206237686 550 VPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILR 616
Cdd:PRK07008 465 CAHPKWDERPLLVVVKRPGAEVTRE---ELLAFYEGKVAKWWIPDDVVFVDAIPHTATGKLQKLKLR 528
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
254-615 |
5.94e-12 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 68.20 E-value: 5.94e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 254 NAEDPLFILYTSGSTGKPKGVVHTTGG-----------YLV----YAAMTF--KYVFDYHpgdIYWCTADVgwVTGHSYL 316
Cdd:cd17648 92 NSTDLAYAIYTSGTTGKPKGVLVEHGSvvnlrtslserYFGrdngDEAVLFfsNYVFDFF---VEQMTLAL--LNGQKLV 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 317 LYGPlacgamtlmfEGVPNYPgvnRLGQVIDKHQVNILYTAPTAIralmaegdKAIEGTKRTSLRIMGSVGEPINPEAwe 396
Cdd:cd17648 167 VPPD----------EMRFDPD---RFYAYINREKVTYLSGTPSVL--------QQYDLARLPHLKRVDAAGEEFTAPV-- 223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 397 wyYNKI-GNGQCPIVDTWWQTETGGF-MITPLPGATELKAgSATRPFFGVQP-ALVDNLGNPQEGASeGNLVITDSwpGQ 473
Cdd:cd17648 224 --FEKLrSRFAGLIINAYGPTETTVTnHKRFFPGDQRFDK-SLGRPVRNTKCyVLNDAMKRVPVGAV-GELYLGGD--GV 297
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 474 ARtlyGDH-------DRFEQTYFST--------FKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVS 538
Cdd:cd17648 298 AR---GYLnrpeltaERFLPNPFQTeqerargrNARLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALAS 374
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 539 HPKIAEAAVVGVPHNIKGQA-----IYAYITLNhgEEPTPElyTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRR 613
Cdd:cd17648 375 YPGVRECAVVAKEDASQAQSriqkyLVGYYLPE--PGHVPE--SDLLSFLRAKLPRYMVPARLVRLEGIPVTINGKLDVR 450
|
..
gi 1206237686 614 IL 615
Cdd:cd17648 451 AL 452
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
103-559 |
6.47e-12 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 68.40 E-value: 6.47e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 103 SQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKL 182
Cdd:cd17639 1 GEYKYMSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQNIPIVTVYATLGEDALIHSLNETECSA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 183 VITadegmragraiplkknvddalkNPaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemNAEDPLFIL 262
Cdd:cd17639 81 IFT----------------------DG--------------------------------------------KPDDLACIM 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 263 YTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDiywctADVgwvtghsYLLYGPLAcGAMTLMFEGVPNYPGVnRL 342
Cdd:cd17639 95 YTSGSTGNPKGVMLTHGN-LVAGIAGLGDRVPELLGP-----DDR-------YLAYLPLA-HIFELAAENVCLYRGG-TI 159
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 343 G-----QVIDKHQVN----------ILYTAPTAI-----RALMAEGD------------------KAIEGTKRTS----- 379
Cdd:cd17639 160 GygsprTLTDKSKRGckgdltefkpTLMVGVPAIwdtirKGVLAKLNpmgglkrtlfwtayqsklKALKEGPGTPlldel 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 380 ------------LRIMGSVGEPINPEAWEWyynkIGNGQCPIVDTWWQTETGGFMITPLPGatELKAGSATRPFFGVQPA 447
Cdd:cd17639 240 vfkkvraalggrLRYMLSGGAPLSADTQEF----LNIVLCPVIQGYGLTETCAGGTVQDPG--DLETGRVGPPLPCCEIK 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 448 LVD--------NLGNPQegaseGNLVITdswpGQARTLyGDHDRFEQTYFSTFKGMYF-SGDGARRDEDGYYWITGRVDD 518
Cdd:cd17639 314 LVDweeggystDKPPPR-----GEILIR----GPNVFK-GYYKNPEKTKEAFDGDGWFhTGDIGEFHPDGTLKIIDRKKD 383
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 1206237686 519 -VLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAI 559
Cdd:cd17639 384 lVKLQNGEYIALEKLESIYRSNPLVNNICVYADPDKSYPVAI 425
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
79-617 |
7.48e-12 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 68.29 E-value: 7.48e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 79 ANCLDRHLTERGEQTAIIwegddpSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAV 158
Cdd:PLN02860 10 CQCLTRLATLRGNAVVTI------SGNRRRTGHEFVDGVLSLAAGLLRLGLRNGDVVAIAALNSDLYLEWLLAVACAGGI 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 159 HSVIFGGFSPDAVAGRIIDSNAKLVITaDEG-------MRAGRAIPLKKNVddALKNPAITSINNVVVFQRTgktgywqe 231
Cdd:PLN02860 84 VAPLNYRWSFEEAKSAMLLVRPVMLVT-DETcsswyeeLQNDRLPSLMWQV--FLESPSSSVFIFLNSFLTT-------- 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 232 grDLWWHDLIVGVSAD-CPAeemnAEDPLFILYTSGSTGKPKGVV--HTTggyLVYAAMTFKYVFDYHPGDIYWCTADVG 308
Cdd:PLN02860 153 --EMLKQRALGTTELDyAWA----PDDAVLICFTSGTTGRPKGVTisHSA---LIVQSLAKIAIVGYGEDDVYLHTAPLC 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 309 WVTGHSyllygplacGAMTLMFEG-----VPNYPGVNRLgQVIDKHQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIM 383
Cdd:PLN02860 224 HIGGLS---------SALAMLMVGachvlLPKFDAKAAL-QAIKQHNVTSMITVPAMMADLISLTRKSMTWKVFPSVRKI 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 384 ----GSVGEPINPEAWEWYYNkigngqCPIVDTWWQTETGG---FM----------ITPLPGATELKAGSATRPFfGV-- 444
Cdd:PLN02860 294 lnggGSLSSRLLPDAKKLFPN------AKLFSAYGMTEACSsltFMtlhdptlespKQTLQTVNQTKSSSVHQPQ-GVcv 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 445 -QPALVDNLGNPQEGASEGNLVITDSWPGQARtlYGDHDRfEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVS 523
Cdd:PLN02860 367 gKPAPHVELKIGLDESSRVGRILTRGPHVMLG--YWGQNS-ETASVLSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTG 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 524 GHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHG------EEPTPE-----------LYTEVRNWVRKE 586
Cdd:PLN02860 444 GENVYPEEVEAVLSQHPGVASVVVVGVPDSRLTEMVVACVRLRDGwiwsdnEKENAKknltlssetlrHHCREKNLSRFK 523
|
570 580 590
....*....|....*....|....*....|.
gi 1206237686 587 IGPIatpdILHWTDSLPKTRSGKIMRRILRK 617
Cdd:PLN02860 524 IPKL----FVQWRKPFPLTTTGKIRRDEVRR 550
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
87-618 |
1.74e-11 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 66.92 E-value: 1.74e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 87 TERGEQTAIIWEGDDPSQSKhVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACarigavhsvIFGGF 166
Cdd:cd05906 20 AERGPTKGITYIDADGSEEF-QSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWAC---------VLAGF 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 167 SPdavagriidsnAKLVITADEGMRAGRAIPLKkNVDDALKNPAI-TSINNVVVFQRtgktgywQEGRDLWWHDLIVGVS 245
Cdd:cd05906 90 VP-----------APLTVPPTYDEPNARLRKLR-HIWQLLGSPVVlTDAELVAEFAG-------LETLSGLPGIRVLSIE 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 246 A--DCPAEEM----NAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKyVFDYHPGDIY--WCTAD-VGWVTgHSYL 316
Cdd:cd05906 151 EllDTAADHDlpqsRPDDLALLMLTSGSTGFPKAVPLTHRNILARSAGKIQ-HNGLTPQDVFlnWVPLDhVGGLV-ELHL 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 317 LygplacgAMTLMFEGVpNYPGVNRLGQV------IDKHQVNILYtAPTAIRALMAegDKAIEGTKRT----SLRIMGSV 386
Cdd:cd05906 229 R-------AVYLGCQQV-HVPTEEILADPlrwldlIDRYRVTITW-APNFAFALLN--DLLEEIEDGTwdlsSLRYLVNA 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 387 GEPINPEAWEWYYNKIGNGQCP---IVDTWWQTETGGFMI----TPLPGAT-ELKAGSATRPFFGVQPALVDNLGNPQEG 458
Cdd:cd05906 298 GEAVVAKTIRRLLRLLEPYGLPpdaIRPAFGMTETCSGVIysrsFPTYDHSqALEFVSLGRPIPGVSMRIVDDEGQLLPE 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 459 ASEGNLVITdswpGQARTL-YGDHDRFEQTYFsTFKGMYFSGDGARRDeDGYYWITGRVDDVLNVSGHRLGTAEIESALV 537
Cdd:cd05906 378 GEVGRLQVR----GPVVTKgYYNNPEANAEAF-TEDGWFRTGDLGFLD-NGNLTITGRTKDTIIVNGVNYYSHEIEAAVE 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 538 SHPKIAEAAVVGVPHNIKGQ-----AIYAYITLNHgEEPTPELYTEVRNWVRKEIG-------PIATPDIlhwtdslPKT 605
Cdd:cd05906 452 EVPGVEPSFTAAFAVRDPGAeteelAIFFVPEYDL-QDALSETLRAIRSVVSREVGvspayliPLPKEEI-------PKT 523
|
570
....*....|...
gi 1206237686 606 RSGKIMRRILRKI 618
Cdd:cd05906 524 SLGKIQRSKLKAA 536
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
109-617 |
4.01e-11 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 65.45 E-value: 4.01e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITade 188
Cdd:cd05940 5 TYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLVV--- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 189 gmragraiplkknvddalknpaitsinnvvvfqrtgktgywqegrdlwwhdlivgvsadcpaeemnaeDPLFILYTSGST 268
Cdd:cd05940 82 --------------------------------------------------------------------DAALYIYTSGTT 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 269 GKPKGVVHTTGGYLVYAAMtFKYVFDYHPGDIYWCTADVGWVTGhsyLLYGPLAC--GAMTLM----FEGvpnypgvNRL 342
Cdd:cd05940 94 GLPKAAIISHRRAWRGGAF-FAGSGGALPSDVLYTCLPLYHSTA---LIVGWSAClaSGATLVirkkFSA-------SNF 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 343 GQVIDKHQVNILYTAPTAIRALMAEgdKAIEGTKRTSLRIMgsVGEPINPEAWEWYYNKIGNGQcpIVDTWWQTE--TGG 420
Cdd:cd05940 163 WDDIRKYQATIFQYIGELCRYLLNQ--PPKPTERKHKVRMI--FGNGLRPDIWEEFKERFGVPR--IAEFYAATEgnSGF 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 421 FMITPLPGAtelkAGSATRPFFGVQP-ALV------DNLGNPQEGASE-------GNLV--ITDSWP------GQARTLY 478
Cdd:cd05940 237 INFFGKPGA----IGRNPSLLRKVAPlALVkydlesGEPIRDAEGRCIkvprgepGLLIsrINPLEPfdgytdPAATEKK 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 479 GDHDRFEQ--TYFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVP-HNIK 555
Cdd:cd05940 313 ILRDVFKKgdAWFNT-------GDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYGVQvPGTD 385
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1206237686 556 GQAIYAYITLnhgEEPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRILRK 617
Cdd:cd05940 386 GRAGMAAIVL---QPNEEFDLSALAAHLEKNLPGYARPLFLRLQPEMEITGTFKQQKVDLRN 444
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
84-551 |
4.62e-11 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 65.69 E-value: 4.62e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 84 RHLT----ERGEQTAII----WEGDDPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARI 155
Cdd:PRK09274 10 RHLPraaqERPDQLAVAvpggRGADGKLAYDELSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALFKA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 156 GAVHSVIFGGFSPDAVAGRIIDSNAKLVITADEGMRAGRAIPLKKnvddalknPAITSINNVvvfqrtgktgywqeGRDL 235
Cdd:PRK09274 90 GAVPVLVDPGMGIKNLKQCLAEAQPDAFIGIPKAHLARRLFGWGK--------PSVRRLVTV--------------GGRL 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 236 WW------HDLIVGVSADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYL--VYAAmtfKYVFDYHPGDIYWCTADV 307
Cdd:PRK09274 148 LWggttlaTLLRDGAAAPFPMADLAPDDMAAILFTSGSTGTPKGVVYTHGMFEaqIEAL---REDYGIEPGEIDLPTFPL 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 308 gwvtghsYLLYGPlACGAMTLMFEGVPNYPG-VN--RLGQVIDKHQVNILYTAPTAIRALMAEGDKAieGTKRTSLRIMG 384
Cdd:PRK09274 225 -------FALFGP-ALGMTSVIPDMDPTRPAtVDpaKLFAAIERYGVTNLFGSPALLERLGRYGEAN--GIKLPSLRRVI 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 385 SVGEPINPEAWEwYYNKIGNGQCPIVDTWWQTETggfmitpLP----GATEL--KAGSATRPFFGVqpaLVdnlGNPQEG 458
Cdd:PRK09274 295 SAGAPVPIAVIE-RFRAMLPPDAEILTPYGATEA-------LPissiESREIlfATRAATDNGAGI---CV---GRPVDG 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 459 ASEGNLVITD----SWPGQARTLYGD---------------HDRFEQTYFS--------TFKGMyfsGDGARRDEDGYYW 511
Cdd:PRK09274 361 VEVRIIAISDapipEWDDALRLATGEigeivvagpmvtrsyYNRPEATRLAkipdgqgdVWHRM---GDLGYLDAQGRLW 437
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 1206237686 512 ITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVP 551
Cdd:PRK09274 438 FCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRSALVGVG 477
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
257-608 |
4.79e-11 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 64.63 E-value: 4.79e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 257 DPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTfkyvfdyhpgdiywctADVGWVTGHS-YLLYGPL------ACGAMTLM 329
Cdd:cd17636 1 DPVLAIYTAAFSGRPNGALLSHQALLAQALVL----------------AVLQAIDEGTvFLNSGPLfhigtlMFTLATFH 64
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 330 FEGVPNY-PGVN--RLGQVIDKHQVNILY-TAPT--AIRALMAEGDKAIegtkrTSLRIMgsvgepinPEAWEWyynkig 403
Cdd:cd17636 65 AGGTNVFvRRVDaeEVLELIEAERCTHAFlLPPTidQIVELNADGLYDL-----SSLRSS--------PAAPEW------ 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 404 NGQCPIVDTWW--------QTETGGFMITPLPGATElkAGSATRPFFGVQPALVDNLGN--PQ----EGASEGNLVITDS 469
Cdd:cd17636 126 NDMATVDTSPWgrkpggygQTEVMGLATFAALGGGA--IGGAGRPSPLVQVRILDEDGRevPDgevgEIVARGPTVMAGY 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 470 WpgqartlygdhDRFEQTYFSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVG 549
Cdd:cd17636 204 W-----------NRPEVNARRTRGGWHHTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIG 272
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 550 VPHNIKGQAIYAYITLNHGEEPTPElytEVRNWVRKEIGPIATPDILHWTDSLPKTRSG 608
Cdd:cd17636 273 VPDPRWAQSVKAIVVLKPGASVTEA---ELIEHCRARIASYKKPKSVEFADALPRTAGG 328
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
255-580 |
5.09e-11 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 65.17 E-value: 5.09e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 255 AEDPLFILYTSGSTGKPKGVVHTTGgylVYAAM--TFKYVFDYHPGDIYWCTADVgwvtghsYLLYGPlACGAMTLMFEG 332
Cdd:cd05910 84 ADEPAAILFTSGSTGTPKGVVYRHG---TFAAQidALRQLYGIRPGEVDLATFPL-------FALFGP-ALGLTSVIPDM 152
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 333 VPNYPG-VN--RLGQVIDKHQVNILYTAPTAIRALMAEGdkAIEGTKRTSLRIMGSVGEPInPEAWEWYYNKIGNGQCPI 409
Cdd:cd05910 153 DPTRPArADpqKLVGAIRQYGVSIVFGSPALLERVARYC--AQHGITLPSLRRVLSAGAPV-PIALAARLRKMLSDEAEI 229
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 410 VDTWWQTET-------GGFMITPLPGATELKAGSAT-RPFFGVQPALVD---------------NLGNPQEGASEGNLVi 466
Cdd:cd05910 230 LTPYGATEAlpvssigSRELLATTTAATSGGAGTCVgRPIPGVRVRIIEiddepiaewddtlelPRGEIGEITVTGPTV- 308
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 467 TDSWPG--QARTLYGDHDRFEQTYFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAE 544
Cdd:cd05910 309 TPTYVNrpVATALAKIDDNSEGFWHRM-------GDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVRR 381
|
330 340 350
....*....|....*....|....*....|....*.
gi 1206237686 545 AAVVGVPHNIKGQAIYAYITLNHGEEPTPELYTEVR 580
Cdd:cd05910 382 SALVGVGKPGCQLPVLCVEPLPGTITPRARLEQELR 417
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
109-322 |
1.62e-10 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 64.37 E-value: 1.62e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVITADE 188
Cdd:PLN02387 108 TYGQVFERVCNFASGLVALGHNKEERVAIFADTRAEWLIALQGCFRQNITVVTIYASLGEEALCHSLNETEVTTVICDSK 187
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 189 gmragraiPLKKNVDDALKnpaITSINNVVVFQRTG--------KTGYWQEGRDLWWHDLivGVSADCPAEEMNAEDPLF 260
Cdd:PLN02387 188 --------QLKKLIDISSQ---LETVKRVIYMDDEGvdsdsslsGSSNWTVSSFSEVEKL--GKENPVDPDLPSPNDIAV 254
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1206237686 261 ILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYHPGDIywctadvgwvtghsYLLYGPLA 322
Cdd:PLN02387 255 IMYTSGSTGLPKGVMMTHGNIVATVAGVMTVVPKLGKNDV--------------YLAYLPLA 302
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
406-625 |
8.86e-10 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 61.16 E-value: 8.86e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 406 QCPIVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVDNlgnpqegaSEGNLVItdswpgQARTL---YGDHD 482
Cdd:PRK07445 254 QLRLAPTYGMTETASQIATLKPDDFLAGNNSSGQVLPHAQITIPAN--------QTGNITI------QAQSLalgYYPQI 319
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 483 RFEQTYFSTfkgmyfsGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAY 562
Cdd:PRK07445 320 LDSQGIFET-------DDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAI 392
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1206237686 563 ITLNHGeEPTPElytEVRNWVRKEIGPIATPDilHW--TDSLPKTRSGKIMRRILRKIAAGDTSN 625
Cdd:PRK07445 393 YVPKDP-SISLE---ELKTAIKDQLSPFKQPK--HWipVPQLPRNPQGKINRQQLQQIAVQRLGL 451
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
497-615 |
1.76e-09 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 60.44 E-value: 1.76e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 497 FSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAYITLNHGEEPtpely 576
Cdd:PRK08308 294 FTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVISHEEIDP----- 368
|
90 100 110
....*....|....*....|....*....|....*....
gi 1206237686 577 TEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:PRK08308 369 VQLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
260-610 |
1.91e-09 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 60.18 E-value: 1.91e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 260 FILYTSGSTGKPKGvVHTTGGYLVYAAMTFKYVFDYHPGDIYWCTA----DVGWVTghsylLYGPLACGAmTLMFegVPN 335
Cdd:cd17654 122 YVIHTSGTTGTPKI-VAVPHKCILPNIQHFRSLFNITSEDILFLTSpltfDPSVVE-----IFLSLSSGA-TLLI--VPT 192
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 336 YPGV--NRLGQVIDK-HQVNILYTAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPInP-----EAWEWYYNKIgngqc 407
Cdd:cd17654 193 SVKVlpSKLADILFKrHRITVLQATPTLFRRFGSQSIKSTVLSATSSLRVLALGGEPF-PslvilSSWRGKGNRT----- 266
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 408 PIVDTWWQTETGGFMIT--------PLPGATELkAGSATRpffgvqpaLVDNLGNPQEGasegnlviTDSWPGQARTLY- 478
Cdd:cd17654 267 RIFNIYGITEVSCWALAykvpeedsPVQLGSPL-LGTVIE--------VRDQNGSEGTG--------QVFLGGLNRVCIl 329
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 479 -GDHDRFEQTyfstfkgMYFSGDGARRdEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIaEAAVVGVPHNikgQ 557
Cdd:cd17654 330 dDEVTVPKGT-------MRATGDFVTV-KDGELFFLGRKDSQIKRRGKRINLDLIQQVIESCLGV-ESCAVTLSDQ---Q 397
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....
gi 1206237686 558 AIYAYITlnhgeepTPELYTEVRNWVRKEIGPI-ATPDILHWTDSLPKTRSGKI 610
Cdd:cd17654 398 RLIAFIV-------GESSSSRIHKELQLTLLSShAIPDTFVQIDKLPLTSHGKV 444
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
106-322 |
1.41e-08 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 58.06 E-value: 1.41e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 106 KHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNAKLVIT 185
Cdd:PTZ00216 120 RYITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALRETECKAIVC 199
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 186 adEGMRAGRAIPLKKNVddALKNPAITSINNVvvfqrtgKTGYWQEGRDL--WWHDLIVGVSA-DC--PAEEMNAEDPLF 260
Cdd:PTZ00216 200 --NGKNVPNLLRLMKSG--GMPNTTIIYLDSL-------PASVDTEGCRLvaWTDVVAKGHSAgSHhpLNIPENNDDLAL 268
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1206237686 261 ILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVF-----DYHPGDIYwCtadvgwvtghSYLlygPLA 322
Cdd:PTZ00216 269 IMYTSGTTGDPKGVMHTHGS-LTAGILALEDRLndligPPEEDETY-C----------SYL---PLA 320
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
108-283 |
6.72e-08 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 55.62 E-value: 6.72e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVagRIIDSNAKLVITAD 187
Cdd:PLN02861 78 LTYKEVYDAAIRIGSAIRSRGVNPGDRCGIYGSNCPEWIIAMEACNSQGITYVPLYDTLGANAV--EFIINHAEVSIAFV 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 EGMRAGRAIPLKKNVDDALKnpAITSINNVVVFQRtgktgywQEGRDLW-----WHDLIVGVSADCPAEEMNAEDPLFIL 262
Cdd:PLN02861 156 QESKISSILSCLPKCSSNLK--TIVSFGDVSSEQK-------EEAEELGvscfsWEEFSLMGSLDCELPPKQKTDICTIM 226
|
170 180
....*....|....*....|.
gi 1206237686 263 YTSGSTGKPKGVVHTTGGYLV 283
Cdd:PLN02861 227 YTSGTTGEPKGVILTNRAIIA 247
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
108-277 |
7.80e-08 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 55.54 E-value: 7.80e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 108 VTYKQLHHNVCQFANVLK-KLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGrIIDSNAKLVITA 186
Cdd:cd17632 68 ITYAELWERVGAVAAAHDpEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAP-ILAETEPRLLAV 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 187 DegmragraiplKKNVDDALKNPA-ITSINNVVVFQRTGKTGYWQE------------GRDLWWHDLIVGVSADCPAEEM 253
Cdd:cd17632 147 S-----------AEHLDLAVEAVLeGGTPPRLVVFDHRPEVDAHRAalesarerlaavGIPVTTLTLIAVRGRDLPPAPL 215
|
170 180
....*....|....*....|....*....
gi 1206237686 254 NAE----DPL-FILYTSGSTGKPKGVVHT 277
Cdd:cd17632 216 FRPepddDPLaLLIYTSGSTGTPKGAMYT 244
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
100-620 |
1.25e-07 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 54.97 E-value: 1.25e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 100 DDPSQSKhVTYKQLhhnvCQFANVL-KKL--GVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIfgGFSpdAVAGRII 176
Cdd:PRK06814 652 EDPVNGP-LTYRKL----LTGAFVLgRKLkkNTPPGENVGVMLPNANGAAVTFFALQSAGRVPAMI--NFS--AGIANIL 722
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 177 DS----NAKLVITAdegmragRAIPLKKNVDdalknPAITSINNVVVFqrtgktgYWQEgrDL-----WWHD---LIVGV 244
Cdd:PRK06814 723 SAckaaQVKTVLTS-------RAFIEKARLG-----PLIEALEFGIRI-------IYLE--DVraqigLADKikgLLAGR 781
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 245 SADCPAEEMNAEDPLFILYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVfDYHPGDIYWCTADVgwvtGHSYLLYGplacG 324
Cdd:PRK06814 782 FPLVYFCNRDPDDPAVILFTSGSEGTPKGVVLSHRNLLANRAQVAARI-DFSPEDKVFNALPV----FHSFGLTG----G 852
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 325 AMTLMFEGVPNYpgvnrlgqvidkhqvniLYTAPTAIRA-----------LMAEGDKAIEGTKRT-------SLRIMGSV 386
Cdd:PRK06814 853 LVLPLLSGVKVF-----------------LYPSPLHYRIipeliydtnatILFGTDTFLNGYARYahpydfrSLRYVFAG 915
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 387 GEPINPEAWEWYYNKIGngqCPIVDTWWQTETGGF--MITPLpgatELKAGSATRPFFGVQPALVdnlgnPQEGASEG-- 462
Cdd:PRK06814 916 AEKVKEETRQTWMEKFG---IRILEGYGVTETAPViaLNTPM----HNKAGTVGRLLPGIEYRLE-----PVPGIDEGgr 983
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 463 ------NLV---ITDSWPGQARTLYGdhdrfeqtyfstfkGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIE 533
Cdd:PRK06814 984 lfvrgpNVMlgyLRAENPGVLEPPAD--------------GWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVE 1049
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 534 SALVSHPKIAEAAVVGVPHNIKGQAIyayITLNHGEEPTPELYTevrNWVRKE-IGPIATPDILHWTDSLPKTRSGKI-- 610
Cdd:PRK06814 1050 ELAAELWPDALHAAVSIPDARKGERI---ILLTTASDATRAAFL---AHAKAAgASELMVPAEIITIDEIPLLGTGKIdy 1123
|
570
....*....|..
gi 1206237686 611 --MRRILRKIAA 620
Cdd:PRK06814 1124 vaVTKLAEEAAA 1135
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
106-322 |
1.93e-07 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 54.33 E-value: 1.93e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 106 KHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVagRIIDSNAKL--- 182
Cdd:PLN02736 77 KWMTYGEAGTARTAIGSGLVQHGIPKGACVGLYFINRPEWLIVDHACSAYSYVSVPLYDTLGPDAV--KFIVNHAEVaai 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 183 ---------VITADEGMRAGRAIPLKKNVDDALKNPAITSINNVVVFQRtgktgYWQEGRdlwwhdliVGVSADCPAEEm 253
Cdd:PLN02736 155 fcvpqtlntLLSCLSEIPSVRLIVVVGGADEPLPSLPSGTGVEIVTYSK-----LLAQGR--------SSPQPFRPPKP- 220
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 254 naEDPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIYWctadvgwvtghSYLlygPLA 322
Cdd:PLN02736 221 --EDVATICYTSGTTGTPKGVVLTHGN-LIANVAGSSLSTKFYPSDVHI-----------SYL---PLA 272
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
109-322 |
4.58e-07 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 52.99 E-value: 4.58e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVK--KGDVVAIYMPMVPEAAVAMLACARIGAVhsvifggfspdavagriidsnaklvita 186
Cdd:cd05927 7 SYKEVAERADNIGSALRSLGGKpaPASFVGIYSINRPEWIISELACYAYSLV---------------------------- 58
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 187 degmragrAIPLKknvdDALKNPAITSINN-----VVVFQRTGKTGYWQEGRDLwwhdlivGVSADCPAEEMNAEDPLFI 261
Cdd:cd05927 59 --------TVPLY----DTLGPEAIEYILNhaeisIVFCDAGVKVYSLEEFEKL-------GKKNKVPPPPPKPEDLATI 119
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1206237686 262 LYTSGSTGKPKGVVHTTGGYLVYAAMTFKYVFDYH---PGDIYWctadvgwvtghSYLlygPLA 322
Cdd:cd05927 120 CYTSGTTGNPKGVMLTHGNIVSNVAGVFKILEILNkinPTDVYI-----------SYL---PLA 169
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
109-277 |
8.82e-07 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 52.12 E-value: 8.82e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVaGRIID-SNAKLVITAD 187
Cdd:PLN02430 78 TYKEVYEEVLQIGSALRASGAEPGSRVGIYGSNCPQWIVAMEACAAHSLICVPLYDTLGPGAV-DYIVDhAEIDFVFVQD 156
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 188 EGMRAgraiPLKKNVDDALKNPAITSINNVVVFQRTGKTgywQEGRDLW-WHDLIvGVSADCPAeEMNAEDPL---FILY 263
Cdd:PLN02430 157 KKIKE----LLEPDCKSAKRLKAIVSFTSVTEEESDKAS---QIGVKTYsWIDFL-HMGKENPS-ETNPPKPLdicTIMY 227
|
170
....*....|....
gi 1206237686 264 TSGSTGKPKGVVHT 277
Cdd:PLN02430 228 TSGTSGDPKGVVLT 241
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
109-615 |
1.51e-06 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 51.05 E-value: 1.51e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 109 TYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIG------AVHSvifggfSPDAVAgRIID-SNAK 181
Cdd:PRK04813 29 TYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGAVKAGhayipvDVSS------PAERIE-MIIEvAKPS 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 182 LVITADEgmragraIPLkknvdDALKNPAITsINNVVVFQRTGKTgywqegrdlwwhdlivgVSADCPaeeMNAEDPLFI 261
Cdd:PRK04813 102 LIIATEE-------LPL-----EILGIPVIT-LDELKDIFATGNP-----------------YDFDHA---VKGDDNYYI 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 262 LYTSGSTGKPKGVVHTTGGYLVYAA-MT--FK------------YVFDYHPGDIYWCTAdvgwvTGhsyllyGPLAC--G 324
Cdd:PRK04813 149 IFTSGTTGKPKGVQISHDNLVSFTNwMLedFAlpegpqflnqapYSFDLSVMDLYPTLA-----SG------GTLVAlpK 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 325 AMT----LMFEGVPNYPgvnrlgqvidkhqVNILYTAPTAIRalMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYN 400
Cdd:PRK04813 218 DMTanfkQLFETLPQLP-------------INVWVSTPSFAD--MCLLDPSFNEEHLPNLTHFLFCGEELPHKTAKKLLE 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 401 KIGNGQcpIVDTWWQTETGGFM----IT--------PLP-GATelKAGSATrpffgvqpALVDNLGNPQEGASEGNLVIT 467
Cdd:PRK04813 283 RFPSAT--IYNTYGPTEATVAVtsieITdemldqykRLPiGYA--KPDSPL--------LIIDEEGTKLPDGEQGEIVIS 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 468 dswpGQARTL--YGDHDRFEQTYFsTFKGM--YFSGDGARRDeDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIA 543
Cdd:PRK04813 351 ----GPSVSKgyLNNPEKTAEAFF-TFDGQpaYHTGDAGYLE-DGLLFYQGRIDFQIKLNGYRIELEEIEQNLRQSSYVE 424
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1206237686 544 EAAVVGVPHNIKGQAIYAYITLNHGE-EPTPELYTEVRNWVRKEIGPIATPDILHWTDSLPKTRSGKIMRRIL 615
Cdd:PRK04813 425 SAVVVPYNKDHKVQYLIAYVVPKEEDfEREFELTKAIKKELKERLMEYMIPRKFIYRDSLPLTPNGKIDRKAL 497
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
254-621 |
3.18e-06 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 50.69 E-value: 3.18e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 254 NAEDPLFILYTSGSTGKPKGVV---HTTGGYLVYAAMtfkyVFDYHPGDIywctadvgwVTG-----HSY----LLYGPL 321
Cdd:PRK08633 780 KPDDTATIIFSSGSEGEPKGVMlshHNILSNIEQISD----VFNLRNDDV---------ILSslpffHSFgltvTLWLPL 846
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 322 ACGAMTLMfegVPNYPGVNRLGQVIDKHQVNILYTAPTAIRALMAegDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNK 401
Cdd:PRK08633 847 LEGIKVVY---HPDPTDALGIAKLVAKHRATILLGTPTFLRLYLR--NKKLHPLMFASLRLVVAGAEKLKPEVADAFEEK 921
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 402 IGNG----------------QCP---IVDTWWQTETG-GFMITPLPGA----------TELKAGSATRPFFGVQPALVDN 451
Cdd:PRK08633 922 FGIRilegygatetspvasvNLPdvlAADFKRQTGSKeGSVGMPLPGVavrivdpetfEELPPGEDGLILIGGPQVMKGY 1001
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 452 LGNPQEGASegnlVITDSwpgqartlygdhdrfeqtyfsTFKGMYFSGDGARRDEDGYYWITGRVDdvlnvsghRLgtAE 531
Cdd:PRK08633 1002 LGDPEKTAE----VIKDI---------------------DGIGWYVTGDKGHLDEDGFLTITDRYS--------RF--AK 1046
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 532 IESALVSHPKIAEA------------AVVGVPHNIKGQAIYAYITlnHGEEPTPELYTEVRNwvrKEIGPIATPDILHWT 599
Cdd:PRK08633 1047 IGGEMVPLGAVEEElakalggeevvfAVTAVPDEKKGEKLVVLHT--CGAEDVEELKRAIKE---SGLPNLWKPSRYFKV 1121
|
410 420
....*....|....*....|..
gi 1206237686 600 DSLPKTRSGKIMRRILRKIAAG 621
Cdd:PRK08633 1122 EALPLLGSGKLDLKGLKELALA 1143
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
101-613 |
6.22e-05 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 46.14 E-value: 6.22e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 101 DPSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIY--MP--MVPEAAVAMLACARIGAVHS----VIFGGFSPDAVa 172
Cdd:PRK07768 23 EPDAPVRHTWGEVHERARRIAGGLAAAGVGPGDAVAVLagAPveIAPTAQGLWMRGASLTMLHQptprTDLAVWAEDTL- 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 173 gRIIDS-NAKLVITADEGMRAGraiplkknvdDALKNPAITSInnvvvfqrtgktgywqEGRDLWWHDlivgvsadcPAE 251
Cdd:PRK07768 102 -RVIGMiGAKAVVVGEPFLAAA----------PVLEEKGIRVL----------------TVADLLAAD---------PID 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 252 --EMNAEDPLFILYTSGSTGKPKGVVHTTGGylVYA---AMTFKYVFDY-HPGDIYW--CTADVGWVTGhsylLYGPLAC 323
Cdd:PRK07768 146 pvETGEDDLALMQLTSGSTGSPKAVQITHGN--LYAnaeAMFVAAEFDVeTDVMVSWlpLFHDMGMVGF----LTVPMYF 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 324 GAmtlmfEGVPNYPgVNRLG------QVIDKHQVNILyTAPTAIRALMAE--GDKAIEGT-KRTSLRIMGSVGEPINPEA 394
Cdd:PRK07768 220 GA-----ELVKVTP-MDFLRdpllwaELISKYRGTMT-AAPNFAYALLARrlRRQAKPGAfDLSSLRFALNGAEPIDPAD 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 395 WEWYYN---KIGNGQCPIVDTWWQTET---------GGFMITPLPGATELKA--------GSATRPFF-------GVQPA 447
Cdd:PRK07768 293 VEDLLDagaRFGLRPEAILPAYGMAEAtlavsfspcGAGLVVDEVDADLLAAlrravpatKGNTRRLAtlgpplpGLEVR 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 448 LVDNLGNPQEGASEGNLVITdswpGQARTL-YGDHDRFEQTyfSTFKGMYFSGDGARRDEDGYYWITGRVDDVLNVSGHR 526
Cdd:PRK07768 373 VVDEDGQVLPPRGVGVIELR----GESVTPgYLTMDGFIPA--QDADGWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRN 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 527 LGTAEIESALVSHPKIAEAAVVGV----PHNIKGQAIYAYITLNHGEEPTPELYTEVRNWVRKEIGpiATPDILHWTD-- 600
Cdd:PRK07768 447 IYPTDIERAAARVEGVRPGNAVAVrldaGHSREGFAVAVESNAFEDPAEVRRIRHQVAHEVVAEVG--VRPRNVVVLGpg 524
|
570
....*....|...
gi 1206237686 601 SLPKTRSGKIMRR 613
Cdd:PRK07768 525 SIPKTPSGKLRRA 537
|
|
| alpha_am_amid |
TIGR03443 |
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ... |
103-274 |
8.36e-05 |
|
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.
Pssm-ID: 274582 [Multi-domain] Cd Length: 1389 Bit Score: 45.83 E-value: 8.36e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 103 SQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDavagR--IIDSNA 180
Cdd:TIGR03443 266 SKTRSFTYKQINEASNILAHYLLKTGIKRGDVVMIYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPA----RqtIYLSVA 341
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 181 K---LVITADegmrAGRAIPL-KKNVDDALKnpAITSINNVVVfqrtGKTGYWQEGRdlwwhdlIVGVSADC--PAEEMN 254
Cdd:TIGR03443 342 KpraLIVIEK----AGTLDQLvRDYIDKELE--LRTEIPALAL----QDDGSLVGGS-------LEGGETDVlaPYQALK 404
|
170 180
....*....|....*....|....*....
gi 1206237686 255 AEDPLFIL---------YTSGSTGKPKGV 274
Cdd:TIGR03443 405 DTPTGVVVgpdsnptlsFTSGSEGIPKGV 433
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
91-393 |
2.12e-04 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 44.40 E-value: 2.12e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 91 EQTAIIWEGDdpSQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACarigavhsvIFGGFSPDA 170
Cdd:cd05908 1 PEGIIFILGD--KKEKFVSYRHLREEALGYLGALQELGIKPGQEVVFQITHNNKFLYLFWAC---------LLGGMIAVP 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 171 VAgriIDSNAklvitaDEGMRAGRAIplkknvdDALKNPAITSINNVvvfqrtgktgyWQEgrdlwwhdlivgvsadcpa 250
Cdd:cd05908 70 VS---IGSNE------EHKLKLNKVW-------NTLKNPYLITEEEV-----------LCE------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 251 eemNAEDPLFILYTSGSTGKPKGVVHTTGGyLVYAAMTFKYVFDYHPGDIY--W--CTADVGWVTGHSYLLYGPLACGAM 326
Cdd:cd05908 104 ---LADELAFIQFSSGSTGDPKGVMLTHEN-LVHNMFAILNSTEWKTKDRIlsWmpLTHDMGLIAFHLAPLIAGMNQYLM 179
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1206237686 327 -TLMFEGVPnypgVNRLGQvIDKHQVNILYTAPTAIRALMAE-GDKAIEGTKRTSLRIMGSVGEPINPE 393
Cdd:cd05908 180 pTRLFIRRP----ILWLKK-ASEHKATIVSSPNFGYKYFLKTlKPEKANDWDLSSIRMILNGAEPIDYE 243
|
|
| A_NRPS_alphaAR |
cd17647 |
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ... |
103-168 |
1.51e-03 |
|
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.
Pssm-ID: 341302 [Multi-domain] Cd Length: 520 Bit Score: 41.73 E-value: 1.51e-03
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1206237686 103 SQSKHVTYKQLHHNVCQFANVLKKLGVKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSP 168
Cdd:cd17647 16 SKTRSFTYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVIDPAYPP 81
|
|
| PRK07868 |
PRK07868 |
acyl-CoA synthetase; Validated |
503-607 |
1.82e-03 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236121 [Multi-domain] Cd Length: 994 Bit Score: 41.63 E-value: 1.82e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1206237686 503 RRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVSHPKIAEAAVVGVPHNIKGQAIYAyITLNHGEEPTPelyTEVRNW 582
Cdd:PRK07868 845 RRDDDGDYWLVDRRGSVIRTARGPVYTEPVTDALGRIGGVDLAVTYGVEVGGRQLAVAA-VTLRPGAAITA---ADLTEA 920
|
90 100
....*....|....*....|....*
gi 1206237686 583 VRkEIGPIATPDILHWTDSLPKTRS 607
Cdd:PRK07868 921 LA-SLPVGLGPDIVHVVPEIPLSAT 944
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
235-277 |
2.53e-03 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 40.85 E-value: 2.53e-03
10 20 30 40
....*....|....*....|....*....|....*....|....*...
gi 1206237686 235 LW--WHDLivgvsadCPAEEM---NAEDPLFILYTSGSTGKPKGVVHT 277
Cdd:PRK08043 346 LWifAHLL-------MPRLAQvkqQPEDAALILFTSGSEGHPKGVVHS 386
|
|
| |
