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Conserved domains on  [gi|116241360|sp|Q9NW38|]
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RecName: Full=E3 ubiquitin-protein ligase FANCL; AltName: Full=Fanconi anemia group L protein; AltName: Full=Fanconi anemia-associated polypeptide of 43 kDa; Short=FAAP43; AltName: Full=RING-type E3 ubiquitin transferase FANCL

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FANCL_d3 pfam18891
FANCL UBC-like domain 3; This entry represents the third of three UBC-like domain found in the ...
 200-295 5.05e-59

FANCL UBC-like domain 3; This entry represents the third of three UBC-like domain found in the FANCL protein, which is the catalytic E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


:

Pssm-ID: 465901  Cd Length: 97  Bit Score: 186.28  E-value: 5.05e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360  200 FWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENS 279
Cdd:pfam18891   2 FWDVLDEIDEKTWVLEPEKPTRSDTSRRIALGNNVSVKIEVDPLHPTALPECKFLGATHVVAPLREKLNDNLHLWDPELS 81

                          90
                  ....*....|....*.
gi 116241360  280 VLQNLKDVLEIDFPAR 295
Cdd:pfam18891  82 LLQNLERVLEIKFPSP 97
FANCL_C pfam11793
FANCL C-terminal domain; This domain is found at the C-terminus of the Fancl protein in humans ...
 303-371 2.77e-40

FANCL C-terminal domain; This domain is found at the C-terminus of the Fancl protein in humans which is the putative E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


:

Pssm-ID: 463350 [Multi-domain]  Cd Length: 69  Bit Score: 136.78  E-value: 2.77e-40
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 116241360  303 FTMDCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNIIFGECPYCSKPITLKMS 371
Cdd:pfam11793   1 FEMECGICYAYRLGGEIPDQVCDNPKCGQPFHRACLYEWLRGLPSSRQSFNVIFGECPYCSKPITVKMS 69
FANCL_d1 pfam09765
FANCL UBC-like domain 1; This entry represents the first of three UBC-like domain found in the ...
 5-93 1.09e-37

FANCL UBC-like domain 1; This entry represents the first of three UBC-like domain found in the FANCL protein, which is the catalytic E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


:

Pssm-ID: 462888  Cd Length: 89  Bit Score: 130.85  E-value: 1.09e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360    5 EASLLRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVLPEDLQLKNARLLCSWQLRTILSGYHRIVQQRMQHSPDLMSFM 84
Cdd:pfam09765   1 MLLLLRKFPLLLPLNRQKTVYDGFIQVKGKDFRLRILLPNDYQLKGAKLYCSWELEYILYGYENILKQRLQSCWDLESFL 80


                  ....*....
gi 116241360   85 MELKMLLEV 93
Cdd:pfam09765  81 LELKTLLER 89
FANCL_d2 pfam18890
FANCL UBC-like domain 2; This entry represents the second of three UBC-like domain found in ...
 108-197 5.50e-35

FANCL UBC-like domain 2; This entry represents the second of three UBC-like domain found in the FANCL protein, which is the catalytic E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


:

Pssm-ID: 465900  Cd Length: 91  Bit Score: 123.51  E-value: 5.50e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360  108 PQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTP-QSSLISI 186
Cdd:pfam18890   1 PNYYSRLLEELETLGWDKLLQIDPDFTTIRLKAEDSSGRQHYLTLKLPSKYPAELPECSHDLPEPFVLEWTPsQSSLEEV 80

                          90
                  ....*....|.
gi 116241360  187 YSQFLAAIESL 197
Cdd:pfam18890  81 LQQFLKVLESL 91
 
Name Accession Description Interval E-value
FANCL_d3 pfam18891
FANCL UBC-like domain 3; This entry represents the third of three UBC-like domain found in the ...
 200-295 5.05e-59

FANCL UBC-like domain 3; This entry represents the third of three UBC-like domain found in the FANCL protein, which is the catalytic E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


Pssm-ID: 465901  Cd Length: 97  Bit Score: 186.28  E-value: 5.05e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360  200 FWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENS 279
Cdd:pfam18891   2 FWDVLDEIDEKTWVLEPEKPTRSDTSRRIALGNNVSVKIEVDPLHPTALPECKFLGATHVVAPLREKLNDNLHLWDPELS 81

                          90
                  ....*....|....*.
gi 116241360  280 VLQNLKDVLEIDFPAR 295
Cdd:pfam18891  82 LLQNLERVLEIKFPSP 97
DRWD-C_FANCL cd23832
C-terminal double-RWD domain of Fanconi anemia group L protein (FANCL) and related proteins; ...
 200-288 1.56e-47

C-terminal double-RWD domain of Fanconi anemia group L protein (FANCL) and related proteins; FANCL, also called E3 ubiquitin-protein ligase FANCL, Fanconi anemia-associated polypeptide of 43 kDa (FAAP43), or RING-type E3 ubiquitin transferase FANCL, is an E3 ubiquitin-protein ligase component of the Fanconi anemia (FA) core complex which functions in the repair of interstrand crosslinks (ICLs), a toxic form of DNA damage. FANCL, works in conjunction with the E2 conjugating enzyme UBE2T (FANCT) to add a single ubiquitin to specific lysine residues on FANCD2 and FANCI. The mono-ubiquitinated FANCI-FANCD2 complex is thought to recruit downstream DNA-repair proteins for ICL processing. FANCL is composed of an N-terminal E2-like fold (ELF) domain, a novel double-RWD (DRWD) domain, and a C-terminal RING domain predicted to facilitate E2 binding. The DRWD domain is responsible for substrate binding. The model corresponds to the C-terminal DRWD domain (DRWD-C).


Pssm-ID: 467666  Cd Length: 89  Bit Score: 156.18  E-value: 1.56e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360 200 FWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENS 279
Cdd:cd23832    1 FWDVLDEIDSNCWVLEPEKPTRSDTYRRIALGNHCSLQITIDPLHPRALPECRFLGPESAVAPLRDKLNRNLSLWDPERS 80


                 ....*....
gi 116241360 280 VLQNLKDVL 288
Cdd:cd23832   81 LLENLERLL 89
FANCL_C pfam11793
FANCL C-terminal domain; This domain is found at the C-terminus of the Fancl protein in humans ...
 303-371 2.77e-40

FANCL C-terminal domain; This domain is found at the C-terminus of the Fancl protein in humans which is the putative E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


Pssm-ID: 463350 [Multi-domain]  Cd Length: 69  Bit Score: 136.78  E-value: 2.77e-40
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 116241360  303 FTMDCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNIIFGECPYCSKPITLKMS 371
Cdd:pfam11793   1 FEMECGICYAYRLGGEIPDQVCDNPKCGQPFHRACLYEWLRGLPSSRQSFNVIFGECPYCSKPITVKMS 69
RING-CH-C4HC3_FANCL cd16490
RING-CH finger, H2 subclass (C4HC3-type), found in Fanconi anemia group L protein (FANCL) and ...
 306-363 9.42e-40

RING-CH finger, H2 subclass (C4HC3-type), found in Fanconi anemia group L protein (FANCL) and similar proteins; FANCL, also known as fanconi anemia-associated polypeptide of 43 kDa (FAAP43) or PHF9, is a monomeric RING E3 ubiquitin-protein ligase that monoubiquitinates FANCD2 and FANCI. The monoubiquitinated FANCD2-FANCI heterodimer complex in turn recruits key proteins involved in homologous recombination and DNA repair. FANCL is also one of seven components in Fanconi anemia (FA) nuclear core complex, which provides the essential E3 ligase function for spatially defined FANCD2 ubiquitination and FA pathway activation. In the FA core complex, FANCL associates with FANCB and FAAP100 to constitute a catalytic subcomplex that functions as the monoubiquitination module. FANCL specifically interacts with the E2 ubiquitin-conjugating (UBC) enzyme Ube2T to make an E3-E2 pair, which is the catalytic center of the Fanconi Anemia (FA) pathway required for DNA interstrand crosslink repair. Moreover, FANCL has a noncanonical function to regulate the Wnt/beta-catenin signaling, a pathway involved in hematopoietic stem cell self-renewal. It functionally enhances beta-catenin activity by ubiquitinating beta-catenin, with atypical ubiquitin chains (K11 linked). FANCL contains an N-terminal E2-like fold (ELF) domain, a novel double-RWD (DRWD) domain with a clear hydrophobic core, and a C-terminal C4HC3-type RING-CH finger. The DRWD domain is required for substrate binding. The RING-CH finger, also known as vRING or RINGv, is predicted to facilitate E2 binding. It has an unusual arrangement of zinc-coordinating residues. Its cysteines and histidines are arranged in the sequence as C4HC3-type, rather than the C3H2C3-type in canonical RING-H2 finger.


Pssm-ID: 438153 [Multi-domain]  Cd Length: 58  Bit Score: 135.07  E-value: 9.42e-40
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 116241360 306 DCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNIIFGECPYCS 363
Cdd:cd16490    1 ECGICYAYRLDGEVPDQVCDNARCGQPFHQSCLYEWLRSLPSTRQSFNTIFGECPYCS 58
FANCL_d1 pfam09765
FANCL UBC-like domain 1; This entry represents the first of three UBC-like domain found in the ...
 5-93 1.09e-37

FANCL UBC-like domain 1; This entry represents the first of three UBC-like domain found in the FANCL protein, which is the catalytic E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


Pssm-ID: 462888  Cd Length: 89  Bit Score: 130.85  E-value: 1.09e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360    5 EASLLRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVLPEDLQLKNARLLCSWQLRTILSGYHRIVQQRMQHSPDLMSFM 84
Cdd:pfam09765   1 MLLLLRKFPLLLPLNRQKTVYDGFIQVKGKDFRLRILLPNDYQLKGAKLYCSWELEYILYGYENILKQRLQSCWDLESFL 80


                  ....*....
gi 116241360   85 MELKMLLEV 93
Cdd:pfam09765  81 LELKTLLER 89
ELF_FANCL cd23786
N-terminal E2-like Fold (ELF) domain of Fanconi anemia group L protein (FANCL) and related ...
 7-92 5.33e-35

N-terminal E2-like Fold (ELF) domain of Fanconi anemia group L protein (FANCL) and related proteins; FANCL, also called E3 ubiquitin-protein ligase FANCL, Fanconi anemia-associated polypeptide of 43 kDa (FAAP43), or RING-type E3 ubiquitin transferase FANCL, is an E3 ubiquitin-protein ligase component of the Fanconi anemia (FA) core complex which functions in the repair of interstrand crosslinks (ICLs), a toxic form of DNA damage. FANCL, works in conjunction with the E2 conjugating enzyme UBE2T (FANCT) to add a single ubiquitin to specific lysine residues on FANCD2 and FANCI. The mono-ubiquitinated FANCI-FANCD2 complex is thought to recruit downstream DNA-repair proteins for ICL processing. FANCL is composed of an N-terminal E2-like fold (ELF) domain, a novel double-RWD (DRWD) domain, and a C-terminal RING domain predicted to facilitate E2 binding. The model corresponds to the ELF domain which cannot bind the substrates.


Pssm-ID: 467648  Cd Length: 86  Bit Score: 123.48  E-value: 5.33e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360   7 SLLRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVLPEDLQLKNARLLCSWQLRTILSGYHRIVQQRMQHSPDLMSFMME 86
Cdd:cd23786    1 SLLLKFPLLLPQNDSGTSYEGFITVKGEEFRIRISLPNDPSLKGASLECDWELKQLLSGYEDILKQRLQQCKSLESFLLE 80


                 ....*.
gi 116241360  87 LKMLLE 92
Cdd:cd23786   81 LKDLLE 86
FANCL_d2 pfam18890
FANCL UBC-like domain 2; This entry represents the second of three UBC-like domain found in ...
 108-197 5.50e-35

FANCL UBC-like domain 2; This entry represents the second of three UBC-like domain found in the FANCL protein, which is the catalytic E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


Pssm-ID: 465900  Cd Length: 91  Bit Score: 123.51  E-value: 5.50e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360  108 PQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTP-QSSLISI 186
Cdd:pfam18890   1 PNYYSRLLEELETLGWDKLLQIDPDFTTIRLKAEDSSGRQHYLTLKLPSKYPAELPECSHDLPEPFVLEWTPsQSSLEEV 80

                          90
                  ....*....|.
gi 116241360  187 YSQFLAAIESL 197
Cdd:pfam18890  81 LQQFLKVLESL 91
DRWD-N_FANCL cd23831
N-terminal double-RWD domain of Fanconi anemia group L protein (FANCL) and related proteins; ...
 116-190 8.61e-26

N-terminal double-RWD domain of Fanconi anemia group L protein (FANCL) and related proteins; FANCL, also called E3 ubiquitin-protein ligase FANCL, Fanconi anemia-associated polypeptide of 43 kDa (FAAP43), or RING-type E3 ubiquitin transferase FANCL, is an E3 ubiquitin-protein ligase component of the Fanconi anemia (FA) core complex which functions in the repair of interstrand crosslinks (ICLs), a toxic form of DNA damage. FANCL, works in conjunction with the E2 conjugating enzyme UBE2T (FANCT) to add a single ubiquitin to specific lysine residues on FANCD2 and FANCI. The mono-ubiquitinated FANCI-FANCD2 complex is thought to recruit downstream DNA-repair proteins for ICL processing. FANCL is composed of an N-terminal E2-like fold (ELF) domain, a novel double-RWD (DRWD) domain, and a C-terminal RING domain predicted to facilitate E2 binding. The DRWD domain is responsible for substrate binding. The model corresponds to the N-terminal DRWD domain (DRWD-N).


Pssm-ID: 467665  Cd Length: 75  Bit Score: 98.82  E-value: 8.61e-26
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 116241360 116 EEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQF 190
Cdd:cd23831    1 EELEAIGWDRVSSIDDDLSSITLKIVDSAGREHLLTVKLPSDYPAEPPTCSADLPEPFEFSWSSSSSLSDIYEQF 75
COG5219 COG5219
Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only];
306-367 5.57e-03

Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only];


Pssm-ID: 227544 [Multi-domain]  Cd Length: 1525  Bit Score: 38.88  E-value: 5.57e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 116241360  306 DCGICYA--YQLDGTIPDQVCdnSQCGQPFHQICLYEWLRGLLTSRqsfniifgeCPYCSKPIT 367
Cdd:COG5219  1471 ECAICYSvlDMVDRSLPSKRC--ATCKNKFHTRCLYKWFASSARSN---------CPLCRSEIT 1523
 
Name Accession Description Interval E-value
FANCL_d3 pfam18891
FANCL UBC-like domain 3; This entry represents the third of three UBC-like domain found in the ...
 200-295 5.05e-59

FANCL UBC-like domain 3; This entry represents the third of three UBC-like domain found in the FANCL protein, which is the catalytic E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


Pssm-ID: 465901  Cd Length: 97  Bit Score: 186.28  E-value: 5.05e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360  200 FWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENS 279
Cdd:pfam18891   2 FWDVLDEIDEKTWVLEPEKPTRSDTSRRIALGNNVSVKIEVDPLHPTALPECKFLGATHVVAPLREKLNDNLHLWDPELS 81

                          90
                  ....*....|....*.
gi 116241360  280 VLQNLKDVLEIDFPAR 295
Cdd:pfam18891  82 LLQNLERVLEIKFPSP 97
DRWD-C_FANCL cd23832
C-terminal double-RWD domain of Fanconi anemia group L protein (FANCL) and related proteins; ...
 200-288 1.56e-47

C-terminal double-RWD domain of Fanconi anemia group L protein (FANCL) and related proteins; FANCL, also called E3 ubiquitin-protein ligase FANCL, Fanconi anemia-associated polypeptide of 43 kDa (FAAP43), or RING-type E3 ubiquitin transferase FANCL, is an E3 ubiquitin-protein ligase component of the Fanconi anemia (FA) core complex which functions in the repair of interstrand crosslinks (ICLs), a toxic form of DNA damage. FANCL, works in conjunction with the E2 conjugating enzyme UBE2T (FANCT) to add a single ubiquitin to specific lysine residues on FANCD2 and FANCI. The mono-ubiquitinated FANCI-FANCD2 complex is thought to recruit downstream DNA-repair proteins for ICL processing. FANCL is composed of an N-terminal E2-like fold (ELF) domain, a novel double-RWD (DRWD) domain, and a C-terminal RING domain predicted to facilitate E2 binding. The DRWD domain is responsible for substrate binding. The model corresponds to the C-terminal DRWD domain (DRWD-C).


Pssm-ID: 467666  Cd Length: 89  Bit Score: 156.18  E-value: 1.56e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360 200 FWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENS 279
Cdd:cd23832    1 FWDVLDEIDSNCWVLEPEKPTRSDTYRRIALGNHCSLQITIDPLHPRALPECRFLGPESAVAPLRDKLNRNLSLWDPERS 80


                 ....*....
gi 116241360 280 VLQNLKDVL 288
Cdd:cd23832   81 LLENLERLL 89
FANCL_C pfam11793
FANCL C-terminal domain; This domain is found at the C-terminus of the Fancl protein in humans ...
 303-371 2.77e-40

FANCL C-terminal domain; This domain is found at the C-terminus of the Fancl protein in humans which is the putative E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


Pssm-ID: 463350 [Multi-domain]  Cd Length: 69  Bit Score: 136.78  E-value: 2.77e-40
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 116241360  303 FTMDCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNIIFGECPYCSKPITLKMS 371
Cdd:pfam11793   1 FEMECGICYAYRLGGEIPDQVCDNPKCGQPFHRACLYEWLRGLPSSRQSFNVIFGECPYCSKPITVKMS 69
RING-CH-C4HC3_FANCL cd16490
RING-CH finger, H2 subclass (C4HC3-type), found in Fanconi anemia group L protein (FANCL) and ...
 306-363 9.42e-40

RING-CH finger, H2 subclass (C4HC3-type), found in Fanconi anemia group L protein (FANCL) and similar proteins; FANCL, also known as fanconi anemia-associated polypeptide of 43 kDa (FAAP43) or PHF9, is a monomeric RING E3 ubiquitin-protein ligase that monoubiquitinates FANCD2 and FANCI. The monoubiquitinated FANCD2-FANCI heterodimer complex in turn recruits key proteins involved in homologous recombination and DNA repair. FANCL is also one of seven components in Fanconi anemia (FA) nuclear core complex, which provides the essential E3 ligase function for spatially defined FANCD2 ubiquitination and FA pathway activation. In the FA core complex, FANCL associates with FANCB and FAAP100 to constitute a catalytic subcomplex that functions as the monoubiquitination module. FANCL specifically interacts with the E2 ubiquitin-conjugating (UBC) enzyme Ube2T to make an E3-E2 pair, which is the catalytic center of the Fanconi Anemia (FA) pathway required for DNA interstrand crosslink repair. Moreover, FANCL has a noncanonical function to regulate the Wnt/beta-catenin signaling, a pathway involved in hematopoietic stem cell self-renewal. It functionally enhances beta-catenin activity by ubiquitinating beta-catenin, with atypical ubiquitin chains (K11 linked). FANCL contains an N-terminal E2-like fold (ELF) domain, a novel double-RWD (DRWD) domain with a clear hydrophobic core, and a C-terminal C4HC3-type RING-CH finger. The DRWD domain is required for substrate binding. The RING-CH finger, also known as vRING or RINGv, is predicted to facilitate E2 binding. It has an unusual arrangement of zinc-coordinating residues. Its cysteines and histidines are arranged in the sequence as C4HC3-type, rather than the C3H2C3-type in canonical RING-H2 finger.


Pssm-ID: 438153 [Multi-domain]  Cd Length: 58  Bit Score: 135.07  E-value: 9.42e-40
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 116241360 306 DCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNIIFGECPYCS 363
Cdd:cd16490    1 ECGICYAYRLDGEVPDQVCDNARCGQPFHQSCLYEWLRSLPSTRQSFNTIFGECPYCS 58
FANCL_d1 pfam09765
FANCL UBC-like domain 1; This entry represents the first of three UBC-like domain found in the ...
 5-93 1.09e-37

FANCL UBC-like domain 1; This entry represents the first of three UBC-like domain found in the FANCL protein, which is the catalytic E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


Pssm-ID: 462888  Cd Length: 89  Bit Score: 130.85  E-value: 1.09e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360    5 EASLLRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVLPEDLQLKNARLLCSWQLRTILSGYHRIVQQRMQHSPDLMSFM 84
Cdd:pfam09765   1 MLLLLRKFPLLLPLNRQKTVYDGFIQVKGKDFRLRILLPNDYQLKGAKLYCSWELEYILYGYENILKQRLQSCWDLESFL 80


                  ....*....
gi 116241360   85 MELKMLLEV 93
Cdd:pfam09765  81 LELKTLLER 89
ELF_FANCL cd23786
N-terminal E2-like Fold (ELF) domain of Fanconi anemia group L protein (FANCL) and related ...
 7-92 5.33e-35

N-terminal E2-like Fold (ELF) domain of Fanconi anemia group L protein (FANCL) and related proteins; FANCL, also called E3 ubiquitin-protein ligase FANCL, Fanconi anemia-associated polypeptide of 43 kDa (FAAP43), or RING-type E3 ubiquitin transferase FANCL, is an E3 ubiquitin-protein ligase component of the Fanconi anemia (FA) core complex which functions in the repair of interstrand crosslinks (ICLs), a toxic form of DNA damage. FANCL, works in conjunction with the E2 conjugating enzyme UBE2T (FANCT) to add a single ubiquitin to specific lysine residues on FANCD2 and FANCI. The mono-ubiquitinated FANCI-FANCD2 complex is thought to recruit downstream DNA-repair proteins for ICL processing. FANCL is composed of an N-terminal E2-like fold (ELF) domain, a novel double-RWD (DRWD) domain, and a C-terminal RING domain predicted to facilitate E2 binding. The model corresponds to the ELF domain which cannot bind the substrates.


Pssm-ID: 467648  Cd Length: 86  Bit Score: 123.48  E-value: 5.33e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360   7 SLLRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVLPEDLQLKNARLLCSWQLRTILSGYHRIVQQRMQHSPDLMSFMME 86
Cdd:cd23786    1 SLLLKFPLLLPQNDSGTSYEGFITVKGEEFRIRISLPNDPSLKGASLECDWELKQLLSGYEDILKQRLQQCKSLESFLLE 80


                 ....*.
gi 116241360  87 LKMLLE 92
Cdd:cd23786   81 LKDLLE 86
FANCL_d2 pfam18890
FANCL UBC-like domain 2; This entry represents the second of three UBC-like domain found in ...
 108-197 5.50e-35

FANCL UBC-like domain 2; This entry represents the second of three UBC-like domain found in the FANCL protein, which is the catalytic E3 ubiquitin ligase subunit of the FA complex (Fanconi anaemia). Eight subunits of the Fanconi anaemia gene products form a multisubunit nuclear complex which is required for mono-ubiquitination of a downstream FA protein, FANCD2.


Pssm-ID: 465900  Cd Length: 91  Bit Score: 123.51  E-value: 5.50e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116241360  108 PQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTP-QSSLISI 186
Cdd:pfam18890   1 PNYYSRLLEELETLGWDKLLQIDPDFTTIRLKAEDSSGRQHYLTLKLPSKYPAELPECSHDLPEPFVLEWTPsQSSLEEV 80

                          90
                  ....*....|.
gi 116241360  187 YSQFLAAIESL 197
Cdd:pfam18890  81 LQQFLKVLESL 91
DRWD-N_FANCL cd23831
N-terminal double-RWD domain of Fanconi anemia group L protein (FANCL) and related proteins; ...
 116-190 8.61e-26

N-terminal double-RWD domain of Fanconi anemia group L protein (FANCL) and related proteins; FANCL, also called E3 ubiquitin-protein ligase FANCL, Fanconi anemia-associated polypeptide of 43 kDa (FAAP43), or RING-type E3 ubiquitin transferase FANCL, is an E3 ubiquitin-protein ligase component of the Fanconi anemia (FA) core complex which functions in the repair of interstrand crosslinks (ICLs), a toxic form of DNA damage. FANCL, works in conjunction with the E2 conjugating enzyme UBE2T (FANCT) to add a single ubiquitin to specific lysine residues on FANCD2 and FANCI. The mono-ubiquitinated FANCI-FANCD2 complex is thought to recruit downstream DNA-repair proteins for ICL processing. FANCL is composed of an N-terminal E2-like fold (ELF) domain, a novel double-RWD (DRWD) domain, and a C-terminal RING domain predicted to facilitate E2 binding. The DRWD domain is responsible for substrate binding. The model corresponds to the N-terminal DRWD domain (DRWD-N).


Pssm-ID: 467665  Cd Length: 75  Bit Score: 98.82  E-value: 8.61e-26
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 116241360 116 EEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQF 190
Cdd:cd23831    1 EELEAIGWDRVSSIDDDLSSITLKIVDSAGREHLLTVKLPSDYPAEPPTCSADLPEPFEFSWSSSSSLSDIYEQF 75
RING-H2 cd16448
H2 subclass of RING (RING-H2) fingers and its variants; The RING finger is a specialized type ...
 307-362 2.50e-05

H2 subclass of RING (RING-H2) fingers and its variants; The RING finger is a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc. It is defined by the "cross-brace" motif that chelates zinc atoms by eight amino acid residues, typically Cys or His, arranged in a characteristic spacing. Canonical RING motifs have been categorized into two major subclasses, RING-HC (C3HC4-type) and RING-H2 (C3H2C3-type), according to their Cys/His content. There are also many variants of RING fingers: some have different Cys/His patterns while some lack a single Cys or His residue at typical Zn ligand positions (the fourth or eighth zinc ligand is prevalently exchanged for an Asp, which can indeed chelate Zn in a RING finger as well). This family corresponds to the H2 subclass of RING (RING-H2) finger proteins that are characterized by containing C3H2C3-type canonical RING-H2 fingers or noncanonical RING-H2 finger variants, including C4HC3- (RING-CH alias RINGv), C3H3C2-, C3H2C2D-, C3DHC3-, and C4HC2H-type modified RING-H2 fingers. The canonical RING-H2 finger has been defined as C-X2-C-X(9-39)-C-X(1-3)-H-X(2-3)-H-X2-C-X(4-48)-C-X2-C, X is any amino acid and the number of X residues varies in different fingers. It binds two Zn ions in a unique "cross-brace" arrangement, which distinguishes it from tandem zinc fingers and other similar motifs. RING-H2 finger can be found in a group of diverse proteins with a variety of cellular functions, including oncogenesis, development, viral replication, signal transduction, the cell cycle and apoptosis. Many of them are ubiquitin-protein ligases (E3s) that serves as a scaffold for binding to ubiquitin-conjugating enzymes (E2s, also referred to as ubiquitin carrier proteins or UBCs) in close proximity to substrate proteins, which enables efficient transfer of ubiquitin from E2 to the substrates.


Pssm-ID: 438112 [Multi-domain]  Cd Length: 43  Bit Score: 41.23  E-value: 2.50e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 116241360 307 CGICYAYQLDGtipdQVCDNSQCGQPFHQICLYEWLRGlltsrqsfniIFGECPYC 362
Cdd:cd16448    1 CVICLEEFEEG----DVVRLLPCGHVFHLACILRWLES----------GNNTCPLC 42
RING-CH-C4HC3_LTN1 cd16491
RING-CH finger, H2 subclass (C4HC3-type), found in E3 ubiquitin-protein ligase listerin and ...
 306-362 3.92e-05

RING-CH finger, H2 subclass (C4HC3-type), found in E3 ubiquitin-protein ligase listerin and similar proteins; Listerin, also known as RING finger protein 160 or zinc finger protein 294, is the mammalian homolog of yeast Ltn1. It is widely expressed in all tissues, but motor and sensory neurons and neuronal processes in the brainstem and spinal cord are primarily affected in the mutant. Listerin is required for embryonic development and plays an important role in neurodegeneration. It also functions as a critical E3 ligase involving quality control of nonstop proteins. It mediates ubiquitylation of aberrant proteins that become stalled on ribosomes during translation. Ltn1 works with several cofactors to form a large ribosomal subunit-associated quality control complex (RQC), which mediates the ubiquitylation and extraction of ribosome-stalled nascent polypeptide chains for proteasomal degradation. It appears to first associate with nascent chain-stalled 60S subunits together with two proteins of unknown function, Tae2 and Rqc1. Listerin contains a long stretch of HEAT (Huntingtin, Elongation factor 3, PR65/A subunit of protein phosphatase 2A, and TOR) or ARM (Armadillo) repeats in the N terminus and middle region, and a catalytic RING-CH finger, also known as vRING or RINGv, with an unusual arrangement of zinc-coordinating residues in the C-terminus . Its cysteines and histidines are arranged in the sequence as C4HC3-type, rather than the C3H2C3-type in canonical RING-H2 finger.


Pssm-ID: 438154 [Multi-domain]  Cd Length: 50  Bit Score: 40.71  E-value: 3.92e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 116241360 306 DCGICYA--YQLDGTIPDQVCdnSQCGQPFHQICLYEWLRgllTSRQSfniifgECPYC 362
Cdd:cd16491    2 ECPICYSviHGSNHSLPKLKC--KTCKNKFHSACLYKWFR---SSNKS------TCPLC 49
COG5219 COG5219
Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only];
306-367 5.57e-03

Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only];


Pssm-ID: 227544 [Multi-domain]  Cd Length: 1525  Bit Score: 38.88  E-value: 5.57e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 116241360  306 DCGICYA--YQLDGTIPDQVCdnSQCGQPFHQICLYEWLRGLLTSRqsfniifgeCPYCSKPIT 367
Cdd:COG5219  1471 ECAICYSvlDMVDRSLPSKRC--ATCKNKFHTRCLYKWFASSARSN---------CPLCRSEIT 1523
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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