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Conserved domains on  [gi|1148298263|ref|WP_077252464|]
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aminomethyltransferase beta-barrel domain-containing protein [Acinetobacter baumannii]

Protein Classification

adenine nucleotide alpha hydrolase family protein( domain architecture ID 188)

AANH (adenine nucleotide alpha hydrolase) family protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MnmA super family cl33902
tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain [Translation, ...
 2-91 5.82e-21

tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain [Translation, ribosomal structure and biogenesis]; tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain is part of the Pathway/BioSystem: tRNA modification


The actual alignment was detected with superfamily member COG0482:

Pssm-ID: 440250 [Multi-domain]  Cd Length: 353  Bit Score: 84.34  E-value: 5.82e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1148298263   2 LYVVQGKDnpkLWKNIVSVSELHLINETSLLNN-NYTASIRYRSQDTPVKVTQNENG-YIFEFSAPQWAPAVGQSLVLFQ 79
Cdd:COG0482   262 VIVGQGEA---LYSRELTAEDVNWISGEPPEEPlRCTAKIRYRQPPVPATLTPLEDGrVRVEFDEPQRAVTPGQSAVFYD 338

                          90
                  ....*....|..
gi 1148298263  80 ENECLGGGVISE 91
Cdd:COG0482   339 GDRVLGGGIIER 350
 
Name Accession Description Interval E-value
MnmA COG0482
tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain [Translation, ...
 2-91 5.82e-21

tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain [Translation, ribosomal structure and biogenesis]; tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440250 [Multi-domain]  Cd Length: 353  Bit Score: 84.34  E-value: 5.82e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1148298263   2 LYVVQGKDnpkLWKNIVSVSELHLINETSLLNN-NYTASIRYRSQDTPVKVTQNENG-YIFEFSAPQWAPAVGQSLVLFQ 79
Cdd:COG0482   262 VIVGQGEA---LYSRELTAEDVNWISGEPPEEPlRCTAKIRYRQPPVPATLTPLEDGrVRVEFDEPQRAVTPGQSAVFYD 338

                          90
                  ....*....|..
gi 1148298263  80 ENECLGGGVISE 91
Cdd:COG0482   339 GDRVLGGGIIER 350
mnmA PRK00143
tRNA-specific 2-thiouridylase MnmA; Reviewed
 2-90 6.45e-21

tRNA-specific 2-thiouridylase MnmA; Reviewed


Pssm-ID: 234664 [Multi-domain]  Cd Length: 346  Bit Score: 84.35  E-value: 6.45e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1148298263   2 LYVVQGkdnPKLWKNIVSVSELHLINETSLLNN-NYTASIRYRSQDTPVKVTQNENGYIFEFSAPQWAPAVGQSLVLFQE 80
Cdd:PRK00143  260 VVVGQG---EALYSRELIASDLNWVGGEPPEEPfECTAKIRYRQKPVPATVELEDDRVEVEFDEPQRAVTPGQAAVFYDG 336

                          90
                  ....*....|
gi 1148298263  81 NECLGGGVIS 90
Cdd:PRK00143  337 DRVLGGGIIE 346
MnmA_TRMU-like cd01998
MnmA/TRMU family 2-thiouridylases and similar proteins; This family is composed of bacterial ...
 2-89 2.20e-20

MnmA/TRMU family 2-thiouridylases and similar proteins; This family is composed of bacterial tRNA-specific 2-thiouridylase MnmA (EC 2.8.1.13) and mitochondrial tRNA-specific 2-thiouridylase 1 (TRMU or MTU1, EC 2.8.1.14). MnmA catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. TRMU/MTU1 catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln); this is required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. This family belongs to the adenine nucleotide alpha hydrolase (AANH) superfamily that also includes other N-type ATP PPases and ATP sulfurylases. It forms an alpha/beta/alpha fold which binds to the adenosine group.


Pssm-ID: 467502 [Multi-domain]  Cd Length: 349  Bit Score: 82.94  E-value: 2.20e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1148298263   2 LYVVQGKdnPKLWKNIVSVSELHLINETSLLN-NNYTASIRYRSQDTPVKVTQNENGYI-FEFSAPQWAPAVGQSLVLFQ 79
Cdd:cd01998   262 VVVGPGH--PALFSDTLRASDLNWISPEPPLEpLECEAKIRYRQPPVPCTVTPLDDGRLkVEFDEPQRAVTPGQAAVFYD 339

                          90
                  ....*....|
gi 1148298263  80 ENECLGGGVI 89
Cdd:cd01998   340 GDEVLGGGII 349
tRNA_Me_trans_C pfam20258
Aminomethyltransferase beta-barrel domain; This domain is found at the C-terminus of tRNA ...
 20-89 1.08e-12

Aminomethyltransferase beta-barrel domain; This domain is found at the C-terminus of tRNA(5-methylaminomethyl-2-thiouridine)-methyltransferase which is involved in the biosynthesis of the modified nucleoside 5-methylaminomethyl-2-thiouridine present in the wobble position of some tRNAs.


Pssm-ID: 466409 [Multi-domain]  Cd Length: 77  Bit Score: 57.67  E-value: 1.08e-12
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1148298263 20 VSELHLINETSLLNN-NYTASIRYRSQDTPVKVTQNENGYIF-EFSAPQWAPAVGQSLVLFQENECLGGGVI 89
Cdd:pfam20258  6 AKDPNWLGDKPPTEPlECTVKVRHRQPPVPCVVELIDDETVEvHFDEPVRAVTPGQAAVFYDGDRCLGGGII 77
 
Name Accession Description Interval E-value
MnmA COG0482
tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain [Translation, ...
 2-91 5.82e-21

tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain [Translation, ribosomal structure and biogenesis]; tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440250 [Multi-domain]  Cd Length: 353  Bit Score: 84.34  E-value: 5.82e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1148298263   2 LYVVQGKDnpkLWKNIVSVSELHLINETSLLNN-NYTASIRYRSQDTPVKVTQNENG-YIFEFSAPQWAPAVGQSLVLFQ 79
Cdd:COG0482   262 VIVGQGEA---LYSRELTAEDVNWISGEPPEEPlRCTAKIRYRQPPVPATLTPLEDGrVRVEFDEPQRAVTPGQSAVFYD 338

                          90
                  ....*....|..
gi 1148298263  80 ENECLGGGVISE 91
Cdd:COG0482   339 GDRVLGGGIIER 350
mnmA PRK00143
tRNA-specific 2-thiouridylase MnmA; Reviewed
 2-90 6.45e-21

tRNA-specific 2-thiouridylase MnmA; Reviewed


Pssm-ID: 234664 [Multi-domain]  Cd Length: 346  Bit Score: 84.35  E-value: 6.45e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1148298263   2 LYVVQGkdnPKLWKNIVSVSELHLINETSLLNN-NYTASIRYRSQDTPVKVTQNENGYIFEFSAPQWAPAVGQSLVLFQE 80
Cdd:PRK00143  260 VVVGQG---EALYSRELIASDLNWVGGEPPEEPfECTAKIRYRQKPVPATVELEDDRVEVEFDEPQRAVTPGQAAVFYDG 336

                          90
                  ....*....|
gi 1148298263  81 NECLGGGVIS 90
Cdd:PRK00143  337 DRVLGGGIIE 346
MnmA_TRMU-like cd01998
MnmA/TRMU family 2-thiouridylases and similar proteins; This family is composed of bacterial ...
 2-89 2.20e-20

MnmA/TRMU family 2-thiouridylases and similar proteins; This family is composed of bacterial tRNA-specific 2-thiouridylase MnmA (EC 2.8.1.13) and mitochondrial tRNA-specific 2-thiouridylase 1 (TRMU or MTU1, EC 2.8.1.14). MnmA catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. TRMU/MTU1 catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln); this is required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. This family belongs to the adenine nucleotide alpha hydrolase (AANH) superfamily that also includes other N-type ATP PPases and ATP sulfurylases. It forms an alpha/beta/alpha fold which binds to the adenosine group.


Pssm-ID: 467502 [Multi-domain]  Cd Length: 349  Bit Score: 82.94  E-value: 2.20e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1148298263   2 LYVVQGKdnPKLWKNIVSVSELHLINETSLLN-NNYTASIRYRSQDTPVKVTQNENGYI-FEFSAPQWAPAVGQSLVLFQ 79
Cdd:cd01998   262 VVVGPGH--PALFSDTLRASDLNWISPEPPLEpLECEAKIRYRQPPVPCTVTPLDDGRLkVEFDEPQRAVTPGQAAVFYD 339

                          90
                  ....*....|
gi 1148298263  80 ENECLGGGVI 89
Cdd:cd01998   340 GDEVLGGGII 349
tRNA_Me_trans_C pfam20258
Aminomethyltransferase beta-barrel domain; This domain is found at the C-terminus of tRNA ...
 20-89 1.08e-12

Aminomethyltransferase beta-barrel domain; This domain is found at the C-terminus of tRNA(5-methylaminomethyl-2-thiouridine)-methyltransferase which is involved in the biosynthesis of the modified nucleoside 5-methylaminomethyl-2-thiouridine present in the wobble position of some tRNAs.


Pssm-ID: 466409 [Multi-domain]  Cd Length: 77  Bit Score: 57.67  E-value: 1.08e-12
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1148298263 20 VSELHLINETSLLNN-NYTASIRYRSQDTPVKVTQNENGYIF-EFSAPQWAPAVGQSLVLFQENECLGGGVI 89
Cdd:pfam20258  6 AKDPNWLGDKPPTEPlECTVKVRHRQPPVPCVVELIDDETVEvHFDEPVRAVTPGQAAVFYDGDRCLGGGII 77
mnmA PRK14665
tRNA-specific 2-thiouridylase MnmA; Provisional
 13-91 1.16e-05

tRNA-specific 2-thiouridylase MnmA; Provisional


Pssm-ID: 173128 [Multi-domain]  Cd Length: 360  Bit Score: 41.84  E-value: 1.16e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1148298263  13 LWKNIVSVSELHLINETSLLN-NNYTASIRYRSQDTPVKVT-QNENGYIFEFSAPQWAPAVGQSLVLFQENECLGGGVIS 90
Cdd:PRK14665  280 LEKTEMWLKDWNIVNESRLLGcDDIIVKIRYRKQENHCTVTiTPDNLLHVQLHEPLTAIAEGQAAAFYKDGLLLGGGIIT 359


                  .
gi 1148298263  91 E 91
Cdd:PRK14665  360 M 360
PRK14664 PRK14664
tRNA-specific 2-thiouridylase MnmA; Provisional
 9-90 3.25e-05

tRNA-specific 2-thiouridylase MnmA; Provisional


Pssm-ID: 173127 [Multi-domain]  Cd Length: 362  Bit Score: 40.32  E-value: 3.25e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1148298263   9 DNPKLWKNIVSVSELHLINETSLLN-NNYTASIRYRSQDTPVKVTQNENGYIF-EFSAPQWAPAVGQSLVLFQENECLGG 86
Cdd:PRK14664  263 DAEQLKAEYMLAEQDNIVDEQELFAcPDLAVRIRYRSRPIPCRVKRLEDGRLLvRFLAEASAIAPGQSAVFYEGRRVLGG 342


                  ....
gi 1148298263  87 GVIS 90
Cdd:PRK14664  343 AFIA 346
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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