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Conserved domains on  [gi|1125431540|gb|OLD60565|]
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hypothetical protein AUF60_00680 [Gemmatimonadetes bacterium 13_1_20CM_69_28]

Protein Classification

polysaccharide deacetylase family protein( domain architecture ID 79029)

metal-dependent polysaccharide deacetylase family protein, belonging to the carbohydrate esterase 4 (CE4) superfamily, may catalyze the N- or O-deacetylation of a substrate such as acetylated chitin, peptidoglycan, and acetylated xylan

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
CE4_SF super family cl15692
Catalytic NodB homology domain of the carbohydrate esterase 4 superfamily; The carbohydrate ...
 5-177 3.14e-21

Catalytic NodB homology domain of the carbohydrate esterase 4 superfamily; The carbohydrate esterase 4 (CE4) superfamily mainly includes chitin deacetylases (EC 3.5.1.41), bacterial peptidoglycan N-acetylglucosamine deacetylases (EC 3.5.1.-), and acetylxylan esterases (EC 3.1.1.72), which catalyze the N- or O-deacetylation of substrates such as acetylated chitin, peptidoglycan, and acetylated xylan, respectively. Members in this superfamily contain a NodB homology domain that adopts a deformed (beta/alpha)8 barrel fold, which encompasses a mononuclear metalloenzyme employing a conserved His-His-Asp zinc-binding triad, closely associated with the conserved catalytic base (aspartic acid) and acid (histidine) to carry out acid/base catalysis. The NodB homology domain of CE4 superfamily is remotely related to the 7-stranded beta/alpha barrel catalytic domain of the superfamily consisting of family 38 glycoside hydrolases (GH38), family 57 heat stable retaining glycoside hydrolases (GH57), lactam utilization protein LamB/YcsF family proteins, and YdjC-family proteins.


The actual alignment was detected with superfamily member cd10929:

Pssm-ID: 472828 [Multi-domain]  Cd Length: 263  Bit Score: 87.71  E-value: 3.14e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431540   5 EPGVTADDITADLGAvscvWRE---RFGAPPVSLVFPRNQV--AFLPVIRACGIRVWRGNEPGWYYDCNESSTNRPLARG 79
Cdd:cd10929    88 EEGQTREVFEADLEA----AKKaasKYGVDLRSFVFPRNQVnvGYLDVLAEHGIKCYRGNEPSWLYQEGGTLKLSLLRRA 163

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431540  80 RRLLDA-VNPRVRHARAVEDDmtRASLFLRTNLPAAAW--ALHCARIRNELD-ALRPPQVFHIWWHDHNLGAAVRQRLGR 155
Cdd:cd10929   164 PRLVDPyVNLSGLVNIPASLF--LFGFFLRPYSKRLAFleDLRLRRIKRGMTyAAKNDGIFHLWWHPHNFGKDTEENLSF 241

                         170       180
                  ....*....|....*....|..
gi 1125431540 156 VEQVCDMVAERCLRRLLVSQSM 177
Cdd:cd10929   242 LEEILEHLAALREEYGLESLTM 263
 
Name Accession Description Interval E-value
CE4_u5 cd10929
Putative catalytic domain of uncharacterized bacterial proteins from the carbohydrate esterase ...
 5-177 3.14e-21

Putative catalytic domain of uncharacterized bacterial proteins from the carbohydrate esterase 4 superfamily; This family corresponds to a group of uncharacterized bacterial proteins with high sequence similarity to the catalytic domain of the six-stranded barrel rhizobial NodB-like proteins, which remove N-linked or O-linked acetyl groups from cell wall polysaccharides and belong to the larger carbohydrate esterase 4 (CE4) superfamily.


Pssm-ID: 200555 [Multi-domain]  Cd Length: 263  Bit Score: 87.71  E-value: 3.14e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431540   5 EPGVTADDITADLGAvscvWRE---RFGAPPVSLVFPRNQV--AFLPVIRACGIRVWRGNEPGWYYDCNESSTNRPLARG 79
Cdd:cd10929    88 EEGQTREVFEADLEA----AKKaasKYGVDLRSFVFPRNQVnvGYLDVLAEHGIKCYRGNEPSWLYQEGGTLKLSLLRRA 163

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431540  80 RRLLDA-VNPRVRHARAVEDDmtRASLFLRTNLPAAAW--ALHCARIRNELD-ALRPPQVFHIWWHDHNLGAAVRQRLGR 155
Cdd:cd10929   164 PRLVDPyVNLSGLVNIPASLF--LFGFFLRPYSKRLAFleDLRLRRIKRGMTyAAKNDGIFHLWWHPHNFGKDTEENLSF 241

                         170       180
                  ....*....|....*....|..
gi 1125431540 156 VEQVCDMVAERCLRRLLVSQSM 177
Cdd:cd10929   242 LEEILEHLAALREEYGLESLTM 263
 
Name Accession Description Interval E-value
CE4_u5 cd10929
Putative catalytic domain of uncharacterized bacterial proteins from the carbohydrate esterase ...
 5-177 3.14e-21

Putative catalytic domain of uncharacterized bacterial proteins from the carbohydrate esterase 4 superfamily; This family corresponds to a group of uncharacterized bacterial proteins with high sequence similarity to the catalytic domain of the six-stranded barrel rhizobial NodB-like proteins, which remove N-linked or O-linked acetyl groups from cell wall polysaccharides and belong to the larger carbohydrate esterase 4 (CE4) superfamily.


Pssm-ID: 200555 [Multi-domain]  Cd Length: 263  Bit Score: 87.71  E-value: 3.14e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431540   5 EPGVTADDITADLGAvscvWRE---RFGAPPVSLVFPRNQV--AFLPVIRACGIRVWRGNEPGWYYDCNESSTNRPLARG 79
Cdd:cd10929    88 EEGQTREVFEADLEA----AKKaasKYGVDLRSFVFPRNQVnvGYLDVLAEHGIKCYRGNEPSWLYQEGGTLKLSLLRRA 163

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431540  80 RRLLDA-VNPRVRHARAVEDDmtRASLFLRTNLPAAAW--ALHCARIRNELD-ALRPPQVFHIWWHDHNLGAAVRQRLGR 155
Cdd:cd10929   164 PRLVDPyVNLSGLVNIPASLF--LFGFFLRPYSKRLAFleDLRLRRIKRGMTyAAKNDGIFHLWWHPHNFGKDTEENLSF 241

                         170       180
                  ....*....|....*....|..
gi 1125431540 156 VEQVCDMVAERCLRRLLVSQSM 177
Cdd:cd10929   242 LEEILEHLAALREEYGLESLTM 263
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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