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Conserved domains on  [gi|1120152707|ref|WP_073022732|]
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GNAT family N-acetyltransferase [Clostridium intestinale]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10456837)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  15581578|10940244|9175471|27367672|26818562
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 49-146 5.14e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


:

Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 51.36  E-value: 5.14e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1120152707  49 NGHSYVLLKDDNIVATVAVSFDGEstynniydgkwitNDKYAVIHRIAVDNTYKGLGLSSEIIKKVEELCVINNVHSIKV 128
Cdd:pfam00583  32 SEGFFVAEEDGELVGFASLSIIDD-------------EPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFL 98

                          90
                  ....*....|....*...
gi 1120152707 129 DTHRENLSMQKLLKKNKF 146
Cdd:pfam00583  99 EVAADNLAAIALYEKLGF 116
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 49-146 5.14e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 51.36  E-value: 5.14e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1120152707  49 NGHSYVLLKDDNIVATVAVSFDGEstynniydgkwitNDKYAVIHRIAVDNTYKGLGLSSEIIKKVEELCVINNVHSIKV 128
Cdd:pfam00583  32 SEGFFVAEEDGELVGFASLSIIDD-------------EPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFL 98

                          90
                  ....*....|....*...
gi 1120152707 129 DTHRENLSMQKLLKKNKF 146
Cdd:pfam00583  99 EVAADNLAAIALYEKLGF 116
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 81-165 4.41e-08

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 48.50  E-value: 4.41e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1120152707  81 GKWITNDKYAVIHRIAVDNTYKGLGLSSEIIKKVEELCVINNVHSIKVDTHRENLSMQKLLKKNKFEYCGIIYLLDGNER 160
Cdd:COG0456     5 LGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNYYGDDA 84


                  ....*
gi 1120152707 161 IAFEK 165
Cdd:COG0456    85 LVMEK 89
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 52-129 1.62e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 40.72  E-value: 1.62e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1120152707  52 SYVLLKDDNIVATVAVSFDGEstynniydgkwitNDKYAVIHRIAVDNTYKGLGLSSEIIKKVEELCVINNVHSIKVD 129
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGS-------------GGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 49-146 5.14e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 51.36  E-value: 5.14e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1120152707  49 NGHSYVLLKDDNIVATVAVSFDGEstynniydgkwitNDKYAVIHRIAVDNTYKGLGLSSEIIKKVEELCVINNVHSIKV 128
Cdd:pfam00583  32 SEGFFVAEEDGELVGFASLSIIDD-------------EPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFL 98

                          90
                  ....*....|....*...
gi 1120152707 129 DTHRENLSMQKLLKKNKF 146
Cdd:pfam00583  99 EVAADNLAAIALYEKLGF 116
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 81-165 4.41e-08

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 48.50  E-value: 4.41e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1120152707  81 GKWITNDKYAVIHRIAVDNTYKGLGLSSEIIKKVEELCVINNVHSIKVDTHRENLSMQKLLKKNKFEYCGIIYLLDGNER 160
Cdd:COG0456     5 LGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNYYGDDA 84


                  ....*
gi 1120152707 161 IAFEK 165
Cdd:COG0456    85 LVMEK 89
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 49-167 6.46e-06

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 43.44  E-value: 6.46e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1120152707  49 NGHSYVLLKDDNIVATVAVSFDGEstynniydgkwitndKYAVIHRIAVDNTYKGLGLSSEIIKKVEELCVINNVHSIKV 128
Cdd:COG1246    27 IGEFWVAEEDGEIVGCAALHPLDE---------------DLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFL 91

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1120152707 129 DTHRENlsmQKLLKKNKFEYCGIIYLLDGNERIAFEKIF 167
Cdd:COG1246    92 LTTSAA---IHFYEKLGFEEIDKEDLPYAKVWQRDSVVM 127
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
3-135 1.36e-05

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 42.77  E-value: 1.36e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1120152707   3 FRKTVKADVDNVMVVIKQAQDYFKEQGInqwqnnypnSDTINNDIVNGHSYVLLKDDNIVATVAVSFdgestynniydGK 82
Cdd:COG3153     1 IRPATPEDAEAIAALLRAAFGPGREAEL---------VDRLREDPAAGLSLVAEDDGEIVGHVALSP-----------VD 60

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1120152707  83 WITNDKYAVIHRIAVDNTYKGLGLSSEIIKKVEELCVINNVHSIKVDTHRENL 135
Cdd:COG3153    61 IDGEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLL 113
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 52-129 1.62e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 40.72  E-value: 1.62e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1120152707  52 SYVLLKDDNIVATVAVSFDGEstynniydgkwitNDKYAVIHRIAVDNTYKGLGLSSEIIKKVEELCVINNVHSIKVD 129
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGS-------------GGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 49-147 1.05e-03

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 36.28  E-value: 1.05e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1120152707  49 NGHSYVLLKDDNIVATVAVSFDGEstynniydgkwitnDKYAVIHRIAVDNTYKGLGLSSEIIKKVEELCVINNVHSIKV 128
Cdd:pfam13508   2 GGRFFVAEDDGKIVGFAALLPLDD--------------EGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLEL 67

                          90
                  ....*....|....*....
gi 1120152707 129 DTHRENlsmQKLLKKNKFE 147
Cdd:pfam13508  68 ETTNRA---AAFYEKLGFE 83
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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