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Conserved domains on  [gi|1093984424|ref|WP_070982854|]
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MULTISPECIES: efflux RND transporter periplasmic adaptor subunit [Pseudoalteromonas]

Protein Classification

efflux RND transporter periplasmic adaptor subunit( domain architecture ID 11436533)

efflux RND (resistance-nodulation-division) transporter periplasmic adaptor subunit, similar to Bacillus subtilis YknX, which is part of an unusual four-component transporter with a role in protection against sporulation-delaying-protein-induced killing

CATH:  2.40.50.100|2.40.420.20|2.40.30.170|1.10.287.470
Gene Ontology:  GO:0016020|GO:0055085|GO:0022857|GO:0042802
PubMed:  32073314
TCDB:  2.A.6

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AcrA COG0845
Multidrug efflux pump subunit AcrA (membrane-fusion protein) [Cell wall/membrane/envelope ...
 53-377 2.92e-61

Multidrug efflux pump subunit AcrA (membrane-fusion protein) [Cell wall/membrane/envelope biogenesis, Defense mechanisms];


:

Pssm-ID: 440606 [Multi-domain]  Cd Length: 324  Bit Score: 200.17  E-value: 2.92e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  53 DHQVVINSYGEVKPYEQTNLSVQVSGEVTYWHpsFIPGGVIARGDVLMRVEKDNYEAALLQAEAELSRARAALieeqALA 132
Cdd:COG0845     7 DVPETVEATGTVEARREVEVRARVSGRVEEVL--VDEGDRVKKGQVLARLDPPDLQAALAQAQAQLAAAQAQL----ELA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 133 DVAADEAKRFPNKK---HTDLFLRKPQLMSAKAAVKSAEAGLKRAQRDLDNCEVVAPYDALVVSRSIGLGQFVSVGTPVA 209
Cdd:COG0845    81 KAELERYKALLKKGavsQQELDQAKAALDQAQAALAAAQAALEQARANLAYTTIRAPFDGVVGERNVEPGQLVSAGTPLF 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 210 QLNNIEAAEVVIPIAGFDSAFLPeriEGIPATVTQSGLNGFTRQAVIDRDLGVVDTQTRMVNLVVRIDDPygieskQPAL 289
Cdd:COG0845   161 TIADLDPLEVEFDVPESDLARLK---VGQPVTVTLDAGPGKTFEGKVTFIDPAVDPATRTVRVRAELPNP------DGLL 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 290 KFGAFVQVDFAGKTLSRIYKLPQDLV----NNQTVWLLTDENKLEPRKVQVIREEGEYFYVNVGLKGNERIVTTVPEYPQ 365
Cdd:COG0845   232 RPGMFVRVRIVLGERENALLVPASAVvrdgGGAYVFVVDADGKVERRPVTLGRRDGDQVEVLSGLKAGDRVVVSGLQRLR 311

                         330
                  ....*....|..
gi 1093984424 366 KGMAVKVAGADS 377
Cdd:COG0845   312 DGAKVRVVEAAA 323
 
Name Accession Description Interval E-value
AcrA COG0845
Multidrug efflux pump subunit AcrA (membrane-fusion protein) [Cell wall/membrane/envelope ...
 53-377 2.92e-61

Multidrug efflux pump subunit AcrA (membrane-fusion protein) [Cell wall/membrane/envelope biogenesis, Defense mechanisms];


Pssm-ID: 440606 [Multi-domain]  Cd Length: 324  Bit Score: 200.17  E-value: 2.92e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  53 DHQVVINSYGEVKPYEQTNLSVQVSGEVTYWHpsFIPGGVIARGDVLMRVEKDNYEAALLQAEAELSRARAALieeqALA 132
Cdd:COG0845     7 DVPETVEATGTVEARREVEVRARVSGRVEEVL--VDEGDRVKKGQVLARLDPPDLQAALAQAQAQLAAAQAQL----ELA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 133 DVAADEAKRFPNKK---HTDLFLRKPQLMSAKAAVKSAEAGLKRAQRDLDNCEVVAPYDALVVSRSIGLGQFVSVGTPVA 209
Cdd:COG0845    81 KAELERYKALLKKGavsQQELDQAKAALDQAQAALAAAQAALEQARANLAYTTIRAPFDGVVGERNVEPGQLVSAGTPLF 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 210 QLNNIEAAEVVIPIAGFDSAFLPeriEGIPATVTQSGLNGFTRQAVIDRDLGVVDTQTRMVNLVVRIDDPygieskQPAL 289
Cdd:COG0845   161 TIADLDPLEVEFDVPESDLARLK---VGQPVTVTLDAGPGKTFEGKVTFIDPAVDPATRTVRVRAELPNP------DGLL 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 290 KFGAFVQVDFAGKTLSRIYKLPQDLV----NNQTVWLLTDENKLEPRKVQVIREEGEYFYVNVGLKGNERIVTTVPEYPQ 365
Cdd:COG0845   232 RPGMFVRVRIVLGERENALLVPASAVvrdgGGAYVFVVDADGKVERRPVTLGRRDGDQVEVLSGLKAGDRVVVSGLQRLR 311

                         330
                  ....*....|..
gi 1093984424 366 KGMAVKVAGADS 377
Cdd:COG0845   312 DGAKVRVVEAAA 323
RND_mfp TIGR01730
RND family efflux transporter, MFP subunit; This model represents the MFP (membrane fusion ...
 44-372 4.27e-35

RND family efflux transporter, MFP subunit; This model represents the MFP (membrane fusion protein) component of the RND family of transporters. RND refers to Resistance, Nodulation, and cell Division. It is, in part, a subfamily of pfam00529 (Pfam release 7.5) but hits substantial numbers of proteins missed by that model. The related HlyD secretion protein, for which pfam00529 is named, is outside the scope of this model. Attributed functions imply outward transport. These functions include nodulation, acriflavin resistance, heavy metal efflux, and multidrug resistance proteins. Most members of this family are found in Gram-negative bacteria. The proposed function of MFP proteins is to bring the inner and outer membranes together and enable transport to the outside of the outer membrane. Note, however, that a few members of this family are found in Gram-positive bacteria, where there is no outer membrane. [Transport and binding proteins, Unknown substrate]


Pssm-ID: 273776 [Multi-domain]  Cd Length: 322  Bit Score: 131.28  E-value: 4.27e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  44 VEVESISALDHQVVINSYGEVKPYEQTNLSVQVSGEVTYWhpSFIPGGVIARGDVLMRVEKDNYEAALLQAEAELSRARA 123
Cdd:TIGR01730   1 VTVATVESETLANTLTFPGSLEAVDEADLAAEVAGKITKI--SVREGQKVKKGQVLARLDDDDYQLALQAALAQLAAAEA 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 124 ALieeqALADVAADEAKRFPNKK---HTDLFLRKPQLMSAKAAVKSAEAGLKRAQRDLDNCEVVAPYDALVVSRSIGLGQ 200
Cdd:TIGR01730  79 QL----ELAQRSFERAERLVKRNavsQADLDDAKAAVEAAQADLEAAKASLASAQLNLRYTEIRAPFDGTIGRRLVEVGA 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 201 FVSVGTPVAQLNNIEAAEVVIPIagfDSAFLPERIEGIPATVTQSGLNGFTRQAVIDRDLGVVDTQTRMVNLVVRIDDPy 280
Cdd:TIGR01730 155 YVTAGQTLATIVDLDPLEADFSV---PERDLPQLRRGQTLTVELDALPGEEFKGKLRFIDPRVDSGTGTVRVRATFPNP- 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 281 gieskQPALKFGAFVQVDFAGKTLSRIYKLPQDLV----NNQTVWLLTDENKLEPRKVQVIREEGEYFYVNVGLKGNERI 356
Cdd:TIGR01730 231 -----DGRLLPGMFGRVTISLKVRSSAIVVPTQAViedlNGKYVYVVKNDGKVSKRPVEVGLRNGGYVEIESGLKAGDQI 305

                         330
                  ....*....|....*.
gi 1093984424 357 VTTVPEYPQKGMAVKV 372
Cdd:TIGR01730 306 VTAGVVKLRDGAKVKV 321
HlyD_3 pfam13437
HlyD family secretion protein; This is a family of largely bacterial haemolysin translocator ...
 182-292 1.61e-12

HlyD family secretion protein; This is a family of largely bacterial haemolysin translocator HlyD proteins.


Pssm-ID: 433206 [Multi-domain]  Cd Length: 104  Bit Score: 63.15  E-value: 1.61e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 182 EVVAPYDALVVSRSIGLGQFVSVGTPVAQLNNIEAAEVVIPIAGFDSAFLPeriEGIPATVTQSGLNGFTRQAVIDRDLG 261
Cdd:pfam13437   1 TIRAPVDGVVAELNVEEGQVVQAGDPLATIVPPDRLLVEAFVPAADLGSLK---KGQKVTLKLDPGSDYTLEGKVVRISP 77

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1093984424 262 VVDTQTRMVNLVVRIDDPYgiesKQPALKFG 292
Cdd:pfam13437  78 TVDPDTGVIPVRVSIENPK----TPIPLLPG 104
PRK09578 PRK09578
MexX/AxyX family multidrug efflux RND transporter periplasmic adaptor subunit;
27-383 7.59e-11

MexX/AxyX family multidrug efflux RND transporter periplasmic adaptor subunit;


Pssm-ID: 169982 [Multi-domain]  Cd Length: 385  Bit Score: 63.27  E-value: 7.59e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  27 VMAADPKEKEPVDSRPVVEVESISALDHQVVINSY--GEVKPYEQTNLSVQVSGEVTywHPSFIPGGVIARGDVLMRVEK 104
Cdd:PRK09578   19 VLAGCGKGDSDAAAAAPREATVVTVRPTSVPMTVElpGRLDAYRQAEVRARVAGIVT--ARTYEEGQEVKQGAVLFRIDP 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 105 DNYEAALLQAEAELSRARAAlieeqALAdvAADEAKRFPN---------KKHTDLFLRKPQlmsAKAAVKSAEAGLKRAQ 175
Cdd:PRK09578   97 APLKAARDAAAGALAKAEAA-----HLA--ALDKRRRYDDlvrdravseRDYTEAVADERQ---AKAAVASAKAELARAQ 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 176 RDLDNCEVVAPYD-----ALVVSRS-IGLGQFVSVgTPVAQLNNI------EAAEVvipiAGFDSAFLPERIEGIP---A 240
Cdd:PRK09578  167 LQLDYATVTAPIDgrarrALVTEGAlVGQDQATPL-TTVEQLDPIyvnfsqPAADV----EALRRAVKSGRATGIAqqdV 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 241 TVTQSGLNGFTRQ---AVIDRDLGvVDTQTRMVNLVVRIDDPygieskQPALKFGAFVQVDFAGKTLSRIYKLPQDLV-- 315
Cdd:PRK09578  242 AVTLVRADGSEYPlkgKLLFSDLA-VDPTTDTVAMRALFPNP------ERELLPGAYVRIALDRAVNPRAILVPRDALlr 314

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 316 --NNQTVWLLTDENKLEPRKVQVIREEGEYFYVNVGLKGNERIVTTVPEYPQKGMAVKVAGADSSDAKAS 383
Cdd:PRK09578  315 taDSASVKVVGQNGKVRDVEVEADQMSGRDWIVTRGLAGGERVIVDNAAQFAPGTAVKAVERAPAAKPAP 384
 
Name Accession Description Interval E-value
AcrA COG0845
Multidrug efflux pump subunit AcrA (membrane-fusion protein) [Cell wall/membrane/envelope ...
 53-377 2.92e-61

Multidrug efflux pump subunit AcrA (membrane-fusion protein) [Cell wall/membrane/envelope biogenesis, Defense mechanisms];


Pssm-ID: 440606 [Multi-domain]  Cd Length: 324  Bit Score: 200.17  E-value: 2.92e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  53 DHQVVINSYGEVKPYEQTNLSVQVSGEVTYWHpsFIPGGVIARGDVLMRVEKDNYEAALLQAEAELSRARAALieeqALA 132
Cdd:COG0845     7 DVPETVEATGTVEARREVEVRARVSGRVEEVL--VDEGDRVKKGQVLARLDPPDLQAALAQAQAQLAAAQAQL----ELA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 133 DVAADEAKRFPNKK---HTDLFLRKPQLMSAKAAVKSAEAGLKRAQRDLDNCEVVAPYDALVVSRSIGLGQFVSVGTPVA 209
Cdd:COG0845    81 KAELERYKALLKKGavsQQELDQAKAALDQAQAALAAAQAALEQARANLAYTTIRAPFDGVVGERNVEPGQLVSAGTPLF 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 210 QLNNIEAAEVVIPIAGFDSAFLPeriEGIPATVTQSGLNGFTRQAVIDRDLGVVDTQTRMVNLVVRIDDPygieskQPAL 289
Cdd:COG0845   161 TIADLDPLEVEFDVPESDLARLK---VGQPVTVTLDAGPGKTFEGKVTFIDPAVDPATRTVRVRAELPNP------DGLL 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 290 KFGAFVQVDFAGKTLSRIYKLPQDLV----NNQTVWLLTDENKLEPRKVQVIREEGEYFYVNVGLKGNERIVTTVPEYPQ 365
Cdd:COG0845   232 RPGMFVRVRIVLGERENALLVPASAVvrdgGGAYVFVVDADGKVERRPVTLGRRDGDQVEVLSGLKAGDRVVVSGLQRLR 311

                         330
                  ....*....|..
gi 1093984424 366 KGMAVKVAGADS 377
Cdd:COG0845   312 DGAKVRVVEAAA 323
RND_mfp TIGR01730
RND family efflux transporter, MFP subunit; This model represents the MFP (membrane fusion ...
 44-372 4.27e-35

RND family efflux transporter, MFP subunit; This model represents the MFP (membrane fusion protein) component of the RND family of transporters. RND refers to Resistance, Nodulation, and cell Division. It is, in part, a subfamily of pfam00529 (Pfam release 7.5) but hits substantial numbers of proteins missed by that model. The related HlyD secretion protein, for which pfam00529 is named, is outside the scope of this model. Attributed functions imply outward transport. These functions include nodulation, acriflavin resistance, heavy metal efflux, and multidrug resistance proteins. Most members of this family are found in Gram-negative bacteria. The proposed function of MFP proteins is to bring the inner and outer membranes together and enable transport to the outside of the outer membrane. Note, however, that a few members of this family are found in Gram-positive bacteria, where there is no outer membrane. [Transport and binding proteins, Unknown substrate]


Pssm-ID: 273776 [Multi-domain]  Cd Length: 322  Bit Score: 131.28  E-value: 4.27e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  44 VEVESISALDHQVVINSYGEVKPYEQTNLSVQVSGEVTYWhpSFIPGGVIARGDVLMRVEKDNYEAALLQAEAELSRARA 123
Cdd:TIGR01730   1 VTVATVESETLANTLTFPGSLEAVDEADLAAEVAGKITKI--SVREGQKVKKGQVLARLDDDDYQLALQAALAQLAAAEA 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 124 ALieeqALADVAADEAKRFPNKK---HTDLFLRKPQLMSAKAAVKSAEAGLKRAQRDLDNCEVVAPYDALVVSRSIGLGQ 200
Cdd:TIGR01730  79 QL----ELAQRSFERAERLVKRNavsQADLDDAKAAVEAAQADLEAAKASLASAQLNLRYTEIRAPFDGTIGRRLVEVGA 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 201 FVSVGTPVAQLNNIEAAEVVIPIagfDSAFLPERIEGIPATVTQSGLNGFTRQAVIDRDLGVVDTQTRMVNLVVRIDDPy 280
Cdd:TIGR01730 155 YVTAGQTLATIVDLDPLEADFSV---PERDLPQLRRGQTLTVELDALPGEEFKGKLRFIDPRVDSGTGTVRVRATFPNP- 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 281 gieskQPALKFGAFVQVDFAGKTLSRIYKLPQDLV----NNQTVWLLTDENKLEPRKVQVIREEGEYFYVNVGLKGNERI 356
Cdd:TIGR01730 231 -----DGRLLPGMFGRVTISLKVRSSAIVVPTQAViedlNGKYVYVVKNDGKVSKRPVEVGLRNGGYVEIESGLKAGDQI 305

                         330
                  ....*....|....*.
gi 1093984424 357 VTTVPEYPQKGMAVKV 372
Cdd:TIGR01730 306 VTAGVVKLRDGAKVKV 321
EmrA COG1566
Multidrug resistance efflux pump EmrA [Defense mechanisms];
56-299 1.65e-15

Multidrug resistance efflux pump EmrA [Defense mechanisms];


Pssm-ID: 441174 [Multi-domain]  Cd Length: 331  Bit Score: 76.63  E-value: 1.65e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  56 VVINSYGEVkPYEQTNLSVQVSGEVTYWHpsFIPGGVIARGDVLMRVEKDNYEAALLQAEAELSRARAALIEEQALA--- 132
Cdd:COG1566    33 EPVTADGRV-EARVVTVAAKVSGRVTEVL--VKEGDRVKKGQVLARLDPTDLQAALAQAEAQLAAAEAQLARLEAELgae 109

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 133 -----------------DVAADEAKRFPN-------------KKHTDL--------------------FLRKPQLMSAKA 162
Cdd:COG1566   110 aeiaaaeaqlaaaqaqlDLAQRELERYQAlykkgavsqqeldEARAALdaaqaqleaaqaqlaqaqagLREEEELAAAQA 189

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 163 AVKSAEAGLKRAQRDLDNCEVVAPYDALVVSRSIGLGQFVSVGTPVAQLNNIEAAEVVIPIAGFDSAFLPeriEGIPATV 242
Cdd:COG1566   190 QVAQAEAALAQAELNLARTTIRAPVDGVVTNLNVEPGEVVSAGQPLLTIVPLDDLWVEAYVPETDLGRVK---PGQPVEV 266

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1093984424 243 TQSGLNGFTRQAVIDR----------DLGVVDTQTRMVNLVVRIDDPYGIEskqpaLKFGAFVQVDF 299
Cdd:COG1566   267 RVDAYPDRVFEGKVTSispgagftspPKNATGNVVQRYPVRIRLDNPDPEP-----LRPGMSATVEI 328
HlyD_3 pfam13437
HlyD family secretion protein; This is a family of largely bacterial haemolysin translocator ...
 182-292 1.61e-12

HlyD family secretion protein; This is a family of largely bacterial haemolysin translocator HlyD proteins.


Pssm-ID: 433206 [Multi-domain]  Cd Length: 104  Bit Score: 63.15  E-value: 1.61e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 182 EVVAPYDALVVSRSIGLGQFVSVGTPVAQLNNIEAAEVVIPIAGFDSAFLPeriEGIPATVTQSGLNGFTRQAVIDRDLG 261
Cdd:pfam13437   1 TIRAPVDGVVAELNVEEGQVVQAGDPLATIVPPDRLLVEAFVPAADLGSLK---KGQKVTLKLDPGSDYTLEGKVVRISP 77

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1093984424 262 VVDTQTRMVNLVVRIDDPYgiesKQPALKFG 292
Cdd:pfam13437  78 TVDPDTGVIPVRVSIENPK----TPIPLLPG 104
CusB_dom_1 pfam00529
Cation efflux system protein CusB domain 1; The cation efflux system protein CusB from E. coli ...
 54-358 1.85e-12

Cation efflux system protein CusB domain 1; The cation efflux system protein CusB from E. coli can be divided into four different domains, the first three domains of the protein are mostly beta-strands and the fourth forms an all alpha-helical domain. This entry represents the first beta-domain (domain 1) of CusB and it is formed by the N and C-terminal ends of the polypeptide (residues 89-102 and 324-385). CusB is part of the copper-transporting efflux system CusCFBA. This domain can also be found in other membrane-fusion proteins, such as HlyD, MdtN, MdtE and AaeA. HlyD is a component of the prototypical alpha-haemolysin (HlyA) bacterial type I secretion system, along with the other components HlyB and TolC. HlyD is anchored in the cytoplasmic membrane by a single transmembrane domain and has a large periplasmic domain within the carboxy-terminal 100 amino acids, HlyB and HlyD form a stable complex that binds the recombinant protein bearing a C-terminal HlyA signal sequence and ATP in the cytoplasm. HlyD, HlyB and TolC combine to form the three-component ABC transporter complex that forms a trans-membrane channel or pore through which HlyA can be transferred directly to the extracellular medium. Cutinase has been shown to be transported effectively through this pore.


Pssm-ID: 425733 [Multi-domain]  Cd Length: 322  Bit Score: 67.45  E-value: 1.85e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  54 HQVVINSYGEVKPY-EQTNLSVQVSGEVTywHPSFIPGGVIARGDVLMRVEKDNYEAALLQAEAELSRARAALIEEQALA 132
Cdd:pfam00529   4 LTKGVEAPGRVVVSgNAKAVQPQVSGIVT--RVLVKEGDRVKAGDVLFQLDPTDYQAALDSAEAQLAKAQAQVARLQAEL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 133 DvAADEAKRFPNKKHTDLFLRKPQLMSAKAAVKSAEAGLKRAQRDLDNCEVVAPYDALVVSRSIGLGQFV-----SVGTP 207
Cdd:pfam00529  82 D-RLQALESELAISRQDYDGATAQLRAAQAAVKAAQAQLAQAQIDLARRRVLAPIGGISRESLVTAGALVaqaqaNLLAT 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 208 VAQLNNIEaAEVVIPIAGFDSAFLPERIEGIP------ATVTQSGLNGFTRQA-------VIDRDL----GVVDTQTRMV 270
Cdd:pfam00529 161 VAQLDQIY-VQITQSAAENQAEVRSELSGAQLqiaeaeAELKLAKLDLERTEIrapvdgtVAFLSVtvdgGTVSAGLRLM 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 271 NLVvriddpygieSKQPALKFGAFVQVDFAG-----KTLSRIYKLPQDLVNNQTVWLLTDENKLEPRKVQVIREEGEYFY 345
Cdd:pfam00529 240 FVV----------PEDNLLVPGMFVETQLDQvrvgqPVLIPFDAFPQTKTGRFTGVVVGISPDTGPVRVVVDKAQGPYYP 309

                         330
                  ....*....|...
gi 1093984424 346 VNVGLKGNERIVT 358
Cdd:pfam00529 310 LRIGLSAGALVRL 322
PRK09578 PRK09578
MexX/AxyX family multidrug efflux RND transporter periplasmic adaptor subunit;
27-383 7.59e-11

MexX/AxyX family multidrug efflux RND transporter periplasmic adaptor subunit;


Pssm-ID: 169982 [Multi-domain]  Cd Length: 385  Bit Score: 63.27  E-value: 7.59e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  27 VMAADPKEKEPVDSRPVVEVESISALDHQVVINSY--GEVKPYEQTNLSVQVSGEVTywHPSFIPGGVIARGDVLMRVEK 104
Cdd:PRK09578   19 VLAGCGKGDSDAAAAAPREATVVTVRPTSVPMTVElpGRLDAYRQAEVRARVAGIVT--ARTYEEGQEVKQGAVLFRIDP 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 105 DNYEAALLQAEAELSRARAAlieeqALAdvAADEAKRFPN---------KKHTDLFLRKPQlmsAKAAVKSAEAGLKRAQ 175
Cdd:PRK09578   97 APLKAARDAAAGALAKAEAA-----HLA--ALDKRRRYDDlvrdravseRDYTEAVADERQ---AKAAVASAKAELARAQ 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 176 RDLDNCEVVAPYD-----ALVVSRS-IGLGQFVSVgTPVAQLNNI------EAAEVvipiAGFDSAFLPERIEGIP---A 240
Cdd:PRK09578  167 LQLDYATVTAPIDgrarrALVTEGAlVGQDQATPL-TTVEQLDPIyvnfsqPAADV----EALRRAVKSGRATGIAqqdV 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 241 TVTQSGLNGFTRQ---AVIDRDLGvVDTQTRMVNLVVRIDDPygieskQPALKFGAFVQVDFAGKTLSRIYKLPQDLV-- 315
Cdd:PRK09578  242 AVTLVRADGSEYPlkgKLLFSDLA-VDPTTDTVAMRALFPNP------ERELLPGAYVRIALDRAVNPRAILVPRDALlr 314

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 316 --NNQTVWLLTDENKLEPRKVQVIREEGEYFYVNVGLKGNERIVTTVPEYPQKGMAVKVAGADSSDAKAS 383
Cdd:PRK09578  315 taDSASVKVVGQNGKVRDVEVEADQMSGRDWIVTRGLAGGERVIVDNAAQFAPGTAVKAVERAPAAKPAP 384
PRK11556 PRK11556
MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit;
75-386 2.81e-10

MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit;


Pssm-ID: 183194 [Multi-domain]  Cd Length: 415  Bit Score: 61.34  E-value: 2.81e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  75 QVSGEVTYWHpsFIPGGVIARGDVLMRVEKDNYEAALLQAEAELSRARAALIeeQALADVAadeakRFPNKKHTDLFLRK 154
Cdd:PRK11556   93 RVDGQLMALH--FQEGQQVKAGDLLAEIDPRPFKVALAQAQGQLAKDQATLA--NARRDLA-----RYQQLAKTNLVSRQ 163

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 155 pQLMSAKAAVKSAEAGLK-------RAQRDLDNCEVVAPYDALVVSRSIGLGQFVSVGTPvaqlNNIeaaeVVI----PI 223
Cdd:PRK11556  164 -ELDAQQALVSETEGTIKadeasvaSAQLQLDYSRITAPISGRVGLKQVDVGNQISSGDT----TGI----VVItqthPI 234

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 224 agfDSAF-LPeriEGIPATVTQSGLNGftrQAVI----DRDLGVVDTQTRMVNLVVRIDDPYG-------IESKQPALKF 291
Cdd:PRK11556  235 ---DLVFtLP---ESDIATVVQAQKAG---KPLVveawDRTNSKKLSEGTLLSLDNQIDATTGtiklkarFNNQDDALFP 305

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 292 GAFVQVDFAGKTLSRIYKLPQDLV----NNQTVWLLTDENKLEPRKVQVIREEGEYFYVNVGLKGNERIVTTVPEYPQKG 367
Cdd:PRK11556  306 NQFVNARMLVDTLQNAVVIPTAALqmgnEGHFVWVLNDENKVSKHLVTPGIQDSQKVVISAGLSAGDRVVTDGIDRLTEG 385

                         330
                  ....*....|....*....
gi 1093984424 368 MAVKVAGADSSDAKASKQT 386
Cdd:PRK11556  386 AKVEVVEPQSATTPEEKAT 404
PRK10476 PRK10476
multidrug transporter subunit MdtN;
93-242 3.46e-10

multidrug transporter subunit MdtN;


Pssm-ID: 182488 [Multi-domain]  Cd Length: 346  Bit Score: 60.81  E-value: 3.46e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  93 IARGDVLMRVEKDNYEAALLQAEAELSRARAAL-------IEEQALADVAADEAKRF-PNKKHTDLFLRKPQLMSAK--- 161
Cdd:PRK10476   70 VKKGDLLFRIDPRPYELTVAQAQADLALADAQImttqrsvDAERSNAASANEQVERArANAKLATRTLERLEPLLAKgyv 149

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 162 ----------------------------------------AAVKSAEAGLKRAQRDLDNCEVVAPYDALVVSRSIGLGQF 201
Cdd:PRK10476  150 saqqvdqartaqrdaevslnqallqaqaaaaavggvdalvAQRAAREAALAIAELHLEDTTVRAPFDGRVVGLKVSVGEF 229

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 1093984424 202 VSVGTPVAQLNNIEAAEVvipIAGFDSAFLPERIEGIPATV 242
Cdd:PRK10476  230 AAPMQPIFTLIDTDHWYA---IANFRETDLKNIRVGDCATV 267
PRK15030 PRK15030
multidrug efflux RND transporter periplasmic adaptor subunit AcrA;
31-383 5.30e-09

multidrug efflux RND transporter periplasmic adaptor subunit AcrA;


Pssm-ID: 184990 [Multi-domain]  Cd Length: 397  Bit Score: 57.42  E-value: 5.30e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  31 DPKEKEPVDSRPVVEVESISALDHQVVINSYGEVKPYEQTNLSVQVSGEVTywHPSFIPGGVIARGDVLMRVEKDNYEAA 110
Cdd:PRK15030   27 DKQAQQGGQQMPAVGVVTVKTEPLQITTELPGRTSAYRIAEVRPQVSGIIL--KRNFKEGSDIEAGVSLYQIDPATYQAT 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 111 LLQAEAELSRARAAlieeqalADVAADEAKRFP--------NKKHTDLFLRKPQlmSAKAAVKSAEAGLKRAQRDLDNCE 182
Cdd:PRK15030  105 YDSAKGDLAKAQAA-------ANIAQLTVNRYQkllgtqyiSKQEYDQALADAQ--QANAAVTAAKAAVETARINLAYTK 175

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 183 VVAPYDALVVSRSIGLGQFVSVGTPVAQLNNIEAAEVVIPIAGFDSAFLPERIEGIPATVTQSglNGFTRQAVIDRDlGV 262
Cdd:PRK15030  176 VTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQE--NGKAKVSLITSD-GI 252

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 263 VDTQTRMVNLV-VRIDDPYG-------IESKQPALKFGAFVQVDFAGKTLSRIYKLPQDLVN-----NQTVWLLTDENKL 329
Cdd:PRK15030  253 KFPQDGTLEFSdVTVDQTTGsitlraiFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTrtprgDATVLVVGADDKV 332

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1093984424 330 EPRKVQVIREEGEYFYVNVGLKGNERIVTTVPEYPQKGMAVKV--AGADSSDAKAS 383
Cdd:PRK15030  333 ETRPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAqeVTADNNQQAAS 388
PRK03598 PRK03598
putative efflux pump membrane fusion protein; Provisional
 61-208 2.70e-07

putative efflux pump membrane fusion protein; Provisional


Pssm-ID: 235136 [Multi-domain]  Cd Length: 331  Bit Score: 51.89  E-value: 2.70e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  61 YGEVKpYEQTNLSVQVSGEVTYWHpsFIPGGVIARGDVLMRVEKDNYEAALLQAEA-------------------ELSRA 121
Cdd:PRK03598   36 YGNVD-IRTVNLGFRVGGRLASLA--VDEGDAVKAGQVLGELDAAPYENALMQAKAnvsvaqaqldlmlagyrdeEIAQA 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 122 RAALIEEQALADVAADEAKR----FPNKKHTDLFL----------------------------RKPQLMSAKAAVKSAEA 169
Cdd:PRK03598  113 RAAVKQAQAAYDYAQNFYNRqqglWKSRTISANDLenarssrdqaqatlksaqdklsqyregnRPQDIAQAKASLAQAQA 192

                         170       180       190
                  ....*....|....*....|....*....|....*....
gi 1093984424 170 GLKRAQRDLDNCEVVAPYDALVVSRSIGLGQFVSVGTPV 208
Cdd:PRK03598  193 ALAQAELNLQDTELIAPSDGTILTRAVEPGTMLNAGSTV 231
PRK09859 PRK09859
multidrug transporter subunit MdtE;
29-385 3.18e-06

multidrug transporter subunit MdtE;


Pssm-ID: 137559 [Multi-domain]  Cd Length: 385  Bit Score: 48.94  E-value: 3.18e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  29 AADPKEKEPVDSR-PVVEVESISALDHQVVINSYGEVKPYEQTNLSVQVSGEVTywHPSFIPGGVIARGDVLMRVEKDNY 107
Cdd:PRK09859   20 ACDDKSAENAAAMtPEVGVVTLSPGSVNVLSELPGRTVPYEVAEIRPQVGGIII--KRNFIEGDKVNQGDSLYQIDPAPL 97

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 108 EAALLQAEAELSRARAAlieeqalADVAADEAKRFPNKKHTDLFLR------KPQLMSAKAAVKSAEAGLKRAQRDLDNC 181
Cdd:PRK09859   98 QAELNSAKGSLAKALST-------ASNARITFNRQASLLKTNYVSRqdydtaRTQLNEAEANVTVAKAAVEQATINLQYA 170

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 182 EVVAPYDALVVSRSIGLGQFVSVGTPVAQLNNIEAAEVVIPIAGFDSAFLP----------ERIEG-IPATVTQSGLNGF 250
Cdd:PRK09859  171 NVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRmkeevasgqiKQVQGsTPVQLNLENGKRY 250

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 251 TRQAVIDRDLGVVDTQTRMVNLVVRIDDPYGieskqpALKFGAFVQVDFAGKTLSRIYKLPQDLVNNQ-----TVWLLTD 325
Cdd:PRK09859  251 SQTGTLKFSDPTVDETTGSVTLRAIFPNPNG------DLLPGMYVTALVDEGSRQNVLLVPQEGVTHNaqgkaTALILDK 324

                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1093984424 326 ENKLEPRKVQVIREEGEYFYVNVGLKGNERIVTTVPEYPQKGMAVKVAGADSSDAKA-SKQ 385
Cdd:PRK09859  325 DDVVQLREIEASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTeSKQ 385
PRK11578 PRK11578
macrolide transporter subunit MacA; Provisional
 109-202 5.50e-05

macrolide transporter subunit MacA; Provisional


Pssm-ID: 183211 [Multi-domain]  Cd Length: 370  Bit Score: 44.77  E-value: 5.50e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 109 AALLQAEAELSRARAALIEEQALADVAA------DEAKrfpnkkhTDLFLRKPQLMSAKAAVKSAEAGLKRAQRDLDNCE 182
Cdd:PRK11578  113 AQRQQAEAELKLARVTLSRQQRLAKTQAvsqqdlDTAA-------TELAVKQAQIGTIDAQIKRNQASLDTAKTNLDYTR 185

                          90       100
                  ....*....|....*....|
gi 1093984424 183 VVAPYDALVVSRSIGLGQFV 202
Cdd:PRK11578  186 IVAPMAGEVTQITTLQGQTV 205
HlyD_D23 pfam16576
Barrel-sandwich domain of CusB or HlyD membrane-fusion; HlyD_D23 is the combined domains 2 and ...
 89-295 8.95e-05

Barrel-sandwich domain of CusB or HlyD membrane-fusion; HlyD_D23 is the combined domains 2 and 3 of the membrane-fusion proteins CusB and HlyD, which forms a barrel-sandwich. CusB and HlyD proteins are membrane fusion proteins of the CusCFBA copper efflux system in E.coli and related bacteria. The whole molecule hinges between D2 and D3. Efflux systems of this resistance-nodulation-division group - RND - have been developed to excrete poisonous metal ions, and in E.coli the only one that deals with silver and copper is the CusA transporter. The transporter CusA works in conjunction with a periplasmic component that is a membrane fusion protein, eg CusB, and an outer-membrane channel component CusC in a CusABC complex driven by import of protons.


Pssm-ID: 435440 [Multi-domain]  Cd Length: 214  Bit Score: 43.26  E-value: 8.95e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  89 PGGVIARGDVLMRVekdnYEAALLQAEAELSRARAALIEEQALADVAADEAKrfpnkkhtdlfLRkpQLMSAKAAVKSae 168
Cdd:pfam16576  38 TGDPVKKGQPLAEL----YSPELVAAQQEYLLALRSGDALSKSELLRAARQR-----------LR--LLGMPEAQIAE-- 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424 169 agLKRAQRDLDNCEVVAPYDALVVSRSIGLGQFVSVGTPVAQLNNIEA----AEVvipiagfdsaflPERI-----EGIP 239
Cdd:pfam16576  99 --LERTGKVQPTVTVYAPISGVVTELNVREGMYVQPGDTLFTIADLSTvwveADV------------PEQDlalvkVGQP 164

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1093984424 240 ATVTQSGLNGFTRQAVIDRDLGVVDTQTRMVnlVVRIDdpygIESKQPALKFGAFV 295
Cdd:pfam16576 165 AEVTLPALPGKTFEGKVDYIYPTLDPKTRTV--RVRIE----LPNPDGRLKPGMFA 214
TolC COG1538
Outer membrane protein TolC [Cell wall/membrane/envelope biogenesis];
91-178 2.04e-03

Outer membrane protein TolC [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441147 [Multi-domain]  Cd Length: 367  Bit Score: 40.02  E-value: 2.04e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1093984424  91 GVIARGDVL-MRVEKDNYEAALLQAEAELSRARAAL-----IEEQALADVAADEAKRFPNKK-----HTDLFLRKPQLMS 159
Cdd:COG1538   116 GLASRLDVLqAEAQLAQARAQLAQAEAQLAQARNALalllgLPPPAPLDLPDPLPPLPPLPPslpglPSEALERRPDLRA 195

                          90
                  ....*....|....*....
gi 1093984424 160 AKAAVKSAEAGLKRAQRDL 178
Cdd:COG1538   196 AEAQLEAAEAEIGVARAAF 214
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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