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Conserved domains on  [gi|1091089802|gb|OHQ23031|]
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glycosyl transferase, partial [Streptococcus sp. HMSC065H07]

Protein Classification

bifunctional glycosyltransferase family 2/GtrA family protein( domain architecture ID 10135401)

bifunctional glycosyltransferase family 2/GtrA family protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DPM_DPG-synthase_like cd04179
DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the ...
 1-155 1.40e-25

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.


:

Pssm-ID: 133022 [Multi-domain]  Cd Length: 185  Bit Score: 100.73  E-value: 1.40e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSgAD--FAPLFAIAQSNYACHLQRHADNKGKGRALKTAISYTLDNFpnakgIVTIDSDGQHTYEDMMKCLAEF 78
Cdd:cd04179    31 IIVVDDGS-TDgtAEIARELAARVPRVRVIRLSRNFGKGAAVRAGFKAARGDI-----VVTMDADLQHPPEDIPKLLEKL 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802  79 LQHPDSLVLGVRTFEKS---VPLRSKFGNLLTRDVLGAMTGVNVSDTQTGLRVIPMSWLPKLLT-VEGERYEFEMNMLLE 154
Cdd:cd04179   105 LEGGADVVIGSRFVRGGgagMPLLRRLGSRLFNFLIRLLLGVRISDTQSGFRLFRREVLEALLSlLESNGFEFGLELLVG 184


                  .
gi 1091089802 155 A 155
Cdd:cd04179   185 A 185
GtrA COG2246
Putative flippase GtrA (transmembrane translocase of bactoprenol-linked glucose) [Lipid ...
 188-312 9.76e-14

Putative flippase GtrA (transmembrane translocase of bactoprenol-linked glucose) [Lipid transport and metabolism];


:

Pssm-ID: 441847  Cd Length: 133  Bit Score: 67.15  E-value: 9.76e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802 188 RIYKVFFKYIFSSLFSFLVDIVAFTILISLFnGLSFsavTLASILARVISSVVNFTLNRKVVFKRGQSNS----AIKYFT 263
Cdd:COG2246     5 RLLRQLLRFGLVGGLGTLVDLGVFYLLVELL-GLPP---LLANVIAFVVAVIVNFLLNRRWTFRSRGGRSrlreFLRFLL 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1091089802 264 LVVVQIVLSSLLVTVLgNALTTLPISLVKILVDGTLFIVSYFVQKHLIF 312
Cdd:COG2246    81 VSLVGLALNLGLLALL-VYVLGLPYLLAKLIGIVVGTVFNFLLSRRWVF 128
 
Name Accession Description Interval E-value
DPM_DPG-synthase_like cd04179
DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the ...
 1-155 1.40e-25

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133022 [Multi-domain]  Cd Length: 185  Bit Score: 100.73  E-value: 1.40e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSgAD--FAPLFAIAQSNYACHLQRHADNKGKGRALKTAISYTLDNFpnakgIVTIDSDGQHTYEDMMKCLAEF 78
Cdd:cd04179    31 IIVVDDGS-TDgtAEIARELAARVPRVRVIRLSRNFGKGAAVRAGFKAARGDI-----VVTMDADLQHPPEDIPKLLEKL 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802  79 LQHPDSLVLGVRTFEKS---VPLRSKFGNLLTRDVLGAMTGVNVSDTQTGLRVIPMSWLPKLLT-VEGERYEFEMNMLLE 154
Cdd:cd04179   105 LEGGADVVIGSRFVRGGgagMPLLRRLGSRLFNFLIRLLLGVRISDTQSGFRLFRREVLEALLSlLESNGFEFGLELLVG 184


                  .
gi 1091089802 155 A 155
Cdd:cd04179   185 A 185
WcaA COG0463
Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis]; ...
 1-190 3.16e-15

Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440231 [Multi-domain]  Cd Length: 208  Bit Score: 73.20  E-value: 3.16e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGADFAPLF-AIAQSNYACHLQRHADNKGKGRALKTAISYtldnfpnAKG--IVTIDSDGQHTYEDMMKCLAE 77
Cdd:COG0463    34 IIVVDDGSTDGTAEILrELAAKDPRIRVIRLERNRGKGAARNAGLAA-------ARGdyIAFLDADDQLDPEKLEELVAA 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802  78 FLQHPDSLVLGVRTFEKSVPLRSKFGNlltRDVLGAMTGVNVSDTQTGLRVIPMSWLPKLLTVEGERYEFEMnmlLEAKE 157
Cdd:COG0463   107 LEEGPADLVYGSRLIREGESDLRRLGS---RLFNLVRLLTNLPDSTSGFRLFRREVLEELGFDEGFLEDTEL---LRALR 180

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1091089802 158 DNIPICEVGIatIYLDENQSSHFdviRDSIRIY 190
Cdd:COG0463   181 HGFRIAEVPV--RYRAGESKLNL---RDLLRLL 208
GtrA COG2246
Putative flippase GtrA (transmembrane translocase of bactoprenol-linked glucose) [Lipid ...
 188-312 9.76e-14

Putative flippase GtrA (transmembrane translocase of bactoprenol-linked glucose) [Lipid transport and metabolism];


Pssm-ID: 441847  Cd Length: 133  Bit Score: 67.15  E-value: 9.76e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802 188 RIYKVFFKYIFSSLFSFLVDIVAFTILISLFnGLSFsavTLASILARVISSVVNFTLNRKVVFKRGQSNS----AIKYFT 263
Cdd:COG2246     5 RLLRQLLRFGLVGGLGTLVDLGVFYLLVELL-GLPP---LLANVIAFVVAVIVNFLLNRRWTFRSRGGRSrlreFLRFLL 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1091089802 264 LVVVQIVLSSLLVTVLgNALTTLPISLVKILVDGTLFIVSYFVQKHLIF 312
Cdd:COG2246    81 VSLVGLALNLGLLALL-VYVLGLPYLLAKLIGIVVGTVFNFLLSRRWVF 128
GtrA pfam04138
GtrA-like protein; Members of this family are predicted to be integral membrane proteins with ...
 195-312 4.91e-12

GtrA-like protein; Members of this family are predicted to be integral membrane proteins with three or four transmembrane spans. They are involved in the synthesis of cell surface polysaccharides. The GtrA family are a subset of this family. GtrA is predicted to be an integral membrane protein with 4 transmembrane spans. It is involved is in O antigen modification by Shigella flexneri bacteriophage X (SfX), but does not determine the specificity of glucosylation. Its function remains unknown, but it may play a role in translocation of undecaprenyl phosphate linked glucose (UndP-Glc) across the cytoplasmic membrane. Another member of this family is a DTDP-glucose-4-keto-6-deoxy-D-glucose reductase, which catalyzes the conversion of dTDP-4-keto-6-deoxy-D-glucose to dTDP-D-fucose, which is involved in the biosynthesis of the serotype-specific polysaccharide antigen of Actinobacillus actinomycetemcomitans Y4 (serotype b). This family also includes the teichoic acid glycosylation protein, GtcA, which is a serotype-specific protein in some Listeria innocua and monocytogenes strains. Its exact function is not known, but it is essential for decoration of cell wall teichoic acids with glucose and galactose.


Pssm-ID: 427738  Cd Length: 116  Bit Score: 61.80  E-value: 4.91e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802 195 KYIFSSLFSFLVDIVAFTILISLFNGlsfsAVTLASILARVISSVVNFTLNRKVVFKRGQSNS---AIKYFTLVVVQIVL 271
Cdd:pfam04138   1 RFLLVGVLGTLVDLAVFLLLLELFGL----SYLLANAIAFVVAILVNFLLNRRWTFRDRRSGSlreLLRFFLVSLAGLLL 76

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1091089802 272 SSLLVTVLGNALTTLPIsLVKILVDGTLFIVSYFVQKHLIF 312
Cdd:pfam04138  77 NLLLLWLLVDGLGLPPL-LAKLVGIAVGTVVNFLLSRFFVF 116
PTZ00260 PTZ00260
dolichyl-phosphate beta-glucosyltransferase; Provisional
 1-194 2.90e-08

dolichyl-phosphate beta-glucosyltransferase; Provisional


Pssm-ID: 240336 [Multi-domain]  Cd Length: 333  Bit Score: 54.39  E-value: 2.90e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGADFAPL---FAIAQSNYACHLQ--RHADNKGKGRALKTAISYTLdnfpnAKGIVTIDSDGQHTYEDMMKCL 75
Cdd:PTZ00260  110 IIIVNDGSKDKTLKVakdFWRQNINPNIDIRllSLLRNKGKGGAVRIGMLASR-----GKYILMVDADGATDIDDFDKLE 184

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802  76 AEFLQHPD---SLVLGVRTF---EKSVPLRSKFGNLLT---RDVLGAMTGVNVSDTQTGLrvipmswlpKLLTVEG---- 142
Cdd:PTZ00260  185 DIMLKIEQnglGIVFGSRNHlvdSDVVAKRKWYRNILMygfHFIVNTICGTNLKDTQCGF---------KLFTRETarii 255

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1091089802 143 ------ERYEFEMNMLLEAKEDNIPICEVGIAtiyLDENQSSHFDVIRDSIRIYKVFF 194
Cdd:PTZ00260  256 fpslhlERWAFDIEIVMIAQKLNLPIAEVPVN---WTEVEGSKLNVISASIQMARDIL 310
Glycos_transf_2 pfam00535
Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, ...
 1-90 3.53e-08

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.


Pssm-ID: 425738 [Multi-domain]  Cd Length: 166  Bit Score: 52.01  E-value: 3.53e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGADF-APLFAIAQSNYACHLQRHADNKGKGRALKTAISYtldnfpnAKG--IVTIDSDGQHTYEDMMKCLAE 77
Cdd:pfam00535  30 IIVVDDGSTDGTvEIAEEYAKKDPRVRVIRLPENRGKAGARNAGLRA-------ATGdyIAFLDADDEVPPDWLEKLVEA 102

                          90
                  ....*....|...
gi 1091089802  78 FLQHPDSLVLGVR 90
Cdd:pfam00535 103 LEEDGADVVVGSR 115
 
Name Accession Description Interval E-value
DPM_DPG-synthase_like cd04179
DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the ...
 1-155 1.40e-25

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133022 [Multi-domain]  Cd Length: 185  Bit Score: 100.73  E-value: 1.40e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSgAD--FAPLFAIAQSNYACHLQRHADNKGKGRALKTAISYTLDNFpnakgIVTIDSDGQHTYEDMMKCLAEF 78
Cdd:cd04179    31 IIVVDDGS-TDgtAEIARELAARVPRVRVIRLSRNFGKGAAVRAGFKAARGDI-----VVTMDADLQHPPEDIPKLLEKL 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802  79 LQHPDSLVLGVRTFEKS---VPLRSKFGNLLTRDVLGAMTGVNVSDTQTGLRVIPMSWLPKLLT-VEGERYEFEMNMLLE 154
Cdd:cd04179   105 LEGGADVVIGSRFVRGGgagMPLLRRLGSRLFNFLIRLLLGVRISDTQSGFRLFRREVLEALLSlLESNGFEFGLELLVG 184


                  .
gi 1091089802 155 A 155
Cdd:cd04179   185 A 185
WcaA COG0463
Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis]; ...
 1-190 3.16e-15

Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440231 [Multi-domain]  Cd Length: 208  Bit Score: 73.20  E-value: 3.16e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGADFAPLF-AIAQSNYACHLQRHADNKGKGRALKTAISYtldnfpnAKG--IVTIDSDGQHTYEDMMKCLAE 77
Cdd:COG0463    34 IIVVDDGSTDGTAEILrELAAKDPRIRVIRLERNRGKGAARNAGLAA-------ARGdyIAFLDADDQLDPEKLEELVAA 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802  78 FLQHPDSLVLGVRTFEKSVPLRSKFGNlltRDVLGAMTGVNVSDTQTGLRVIPMSWLPKLLTVEGERYEFEMnmlLEAKE 157
Cdd:COG0463   107 LEEGPADLVYGSRLIREGESDLRRLGS---RLFNLVRLLTNLPDSTSGFRLFRREVLEELGFDEGFLEDTEL---LRALR 180

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1091089802 158 DNIPICEVGIatIYLDENQSSHFdviRDSIRIY 190
Cdd:COG0463   181 HGFRIAEVPV--RYRAGESKLNL---RDLLRLL 208
GtrA COG2246
Putative flippase GtrA (transmembrane translocase of bactoprenol-linked glucose) [Lipid ...
 188-312 9.76e-14

Putative flippase GtrA (transmembrane translocase of bactoprenol-linked glucose) [Lipid transport and metabolism];


Pssm-ID: 441847  Cd Length: 133  Bit Score: 67.15  E-value: 9.76e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802 188 RIYKVFFKYIFSSLFSFLVDIVAFTILISLFnGLSFsavTLASILARVISSVVNFTLNRKVVFKRGQSNS----AIKYFT 263
Cdd:COG2246     5 RLLRQLLRFGLVGGLGTLVDLGVFYLLVELL-GLPP---LLANVIAFVVAVIVNFLLNRRWTFRSRGGRSrlreFLRFLL 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1091089802 264 LVVVQIVLSSLLVTVLgNALTTLPISLVKILVDGTLFIVSYFVQKHLIF 312
Cdd:COG2246    81 VSLVGLALNLGLLALL-VYVLGLPYLLAKLIGIVVGTVFNFLLSRRWVF 128
GtrA pfam04138
GtrA-like protein; Members of this family are predicted to be integral membrane proteins with ...
 195-312 4.91e-12

GtrA-like protein; Members of this family are predicted to be integral membrane proteins with three or four transmembrane spans. They are involved in the synthesis of cell surface polysaccharides. The GtrA family are a subset of this family. GtrA is predicted to be an integral membrane protein with 4 transmembrane spans. It is involved is in O antigen modification by Shigella flexneri bacteriophage X (SfX), but does not determine the specificity of glucosylation. Its function remains unknown, but it may play a role in translocation of undecaprenyl phosphate linked glucose (UndP-Glc) across the cytoplasmic membrane. Another member of this family is a DTDP-glucose-4-keto-6-deoxy-D-glucose reductase, which catalyzes the conversion of dTDP-4-keto-6-deoxy-D-glucose to dTDP-D-fucose, which is involved in the biosynthesis of the serotype-specific polysaccharide antigen of Actinobacillus actinomycetemcomitans Y4 (serotype b). This family also includes the teichoic acid glycosylation protein, GtcA, which is a serotype-specific protein in some Listeria innocua and monocytogenes strains. Its exact function is not known, but it is essential for decoration of cell wall teichoic acids with glucose and galactose.


Pssm-ID: 427738  Cd Length: 116  Bit Score: 61.80  E-value: 4.91e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802 195 KYIFSSLFSFLVDIVAFTILISLFNGlsfsAVTLASILARVISSVVNFTLNRKVVFKRGQSNS---AIKYFTLVVVQIVL 271
Cdd:pfam04138   1 RFLLVGVLGTLVDLAVFLLLLELFGL----SYLLANAIAFVVAILVNFLLNRRWTFRDRRSGSlreLLRFFLVSLAGLLL 76

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1091089802 272 SSLLVTVLGNALTTLPIsLVKILVDGTLFIVSYFVQKHLIF 312
Cdd:pfam04138  77 NLLLLWLLVDGLGLPPL-LAKLVGIAVGTVVNFLLSRFFVF 116
DPM1_like cd06442
DPM1_like represents putative enzymes similar to eukaryotic DPM1; Proteins similar to ...
 1-167 6.82e-09

DPM1_like represents putative enzymes similar to eukaryotic DPM1; Proteins similar to eukaryotic DPM1, including enzymes from bacteria and archaea; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133062 [Multi-domain]  Cd Length: 224  Bit Score: 55.23  E-value: 6.82e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGS--G-ADFAplFAIAQSNYACHLQRHADNKGKGRALKTAISYTLDNFpnakgIVTIDSDGQHTYEDMMKCLAE 77
Cdd:cd06442    30 IIVVDDNSpdGtAEIV--RELAKEYPRVRLIVRPGKRGLGSAYIEGFKAARGDV-----IVVMDADLSHPPEYIPELLEA 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802  78 FLQHPDSLVLGVR-----TFEKSVPLR---SKFGNLLTRdvlgAMTGVNVSDTQTGLRVIPMSWLPKLL-TVEGERYEFE 148
Cdd:cd06442   103 QLEGGADLVIGSRyveggGVEGWGLKRkliSRGANLLAR----LLLGRKVSDPTSGFRAYRREVLEKLIdSLVSKGYKFQ 178

                         170
                  ....*....|....*....
gi 1091089802 149 MNMLLEAKEDNIPICEVGI 167
Cdd:cd06442   179 LELLVRARRLGYRIVEVPI 197
PTZ00260 PTZ00260
dolichyl-phosphate beta-glucosyltransferase; Provisional
 1-194 2.90e-08

dolichyl-phosphate beta-glucosyltransferase; Provisional


Pssm-ID: 240336 [Multi-domain]  Cd Length: 333  Bit Score: 54.39  E-value: 2.90e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGADFAPL---FAIAQSNYACHLQ--RHADNKGKGRALKTAISYTLdnfpnAKGIVTIDSDGQHTYEDMMKCL 75
Cdd:PTZ00260  110 IIIVNDGSKDKTLKVakdFWRQNINPNIDIRllSLLRNKGKGGAVRIGMLASR-----GKYILMVDADGATDIDDFDKLE 184

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802  76 AEFLQHPD---SLVLGVRTF---EKSVPLRSKFGNLLT---RDVLGAMTGVNVSDTQTGLrvipmswlpKLLTVEG---- 142
Cdd:PTZ00260  185 DIMLKIEQnglGIVFGSRNHlvdSDVVAKRKWYRNILMygfHFIVNTICGTNLKDTQCGF---------KLFTRETarii 255

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1091089802 143 ------ERYEFEMNMLLEAKEDNIPICEVGIAtiyLDENQSSHFDVIRDSIRIYKVFF 194
Cdd:PTZ00260  256 fpslhlERWAFDIEIVMIAQKLNLPIAEVPVN---WTEVEGSKLNVISASIQMARDIL 310
Glycos_transf_2 pfam00535
Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, ...
 1-90 3.53e-08

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.


Pssm-ID: 425738 [Multi-domain]  Cd Length: 166  Bit Score: 52.01  E-value: 3.53e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGADF-APLFAIAQSNYACHLQRHADNKGKGRALKTAISYtldnfpnAKG--IVTIDSDGQHTYEDMMKCLAE 77
Cdd:pfam00535  30 IIVVDDGSTDGTvEIAEEYAKKDPRVRVIRLPENRGKAGARNAGLRA-------ATGdyIAFLDADDEVPPDWLEKLVEA 102

                          90
                  ....*....|...
gi 1091089802  78 FLQHPDSLVLGVR 90
Cdd:pfam00535 103 LEEDGADVVVGSR 115
DPG_synthase cd04188
DPG_synthase is involved in protein N-linked glycosylation; UDP-glucose:dolichyl-phosphate ...
 1-167 7.28e-08

DPG_synthase is involved in protein N-linked glycosylation; UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate.


Pssm-ID: 133031 [Multi-domain]  Cd Length: 211  Bit Score: 52.18  E-value: 7.28e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGADFAP-LFAIAQSNYA----CHLQRhadNKGKGRALKTAISYTLDNFpnakgIVTIDSDGQHTYEDMMKCL 75
Cdd:cd04188    33 IIVVDDGSKDGTAEvARKLARKNPAlirvLTLPK---NRGKGGAVRAGMLAARGDY-----ILFADADLATPFEELEKLE 104

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802  76 AEFLQHPDSLVLGVR-TFEKSV-----PLR---SKFGNLLTRDVLGAmtgvNVSDTQTGLRVIPMSWLPKLLT-VEGERY 145
Cdd:cd04188   105 EALKTSGYDIAIGSRaHLASAAvvkrsWLRnllGRGFNFLVRLLLGL----GIKDTQCGFKLFTRDAARRLFPrLHLERW 180

                         170       180
                  ....*....|....*....|..
gi 1091089802 146 EFEMNMLLEAKEDNIPICEVGI 167
Cdd:cd04188   181 AFDVELLVLARRLGYPIEEVPV 202
DPM1_like_bac cd04187
Bacterial DPM1_like enzymes are related to eukaryotic DPM1; A family of bacterial enzymes ...
 1-129 5.86e-06

Bacterial DPM1_like enzymes are related to eukaryotic DPM1; A family of bacterial enzymes related to eukaryotic DPM1; Although the mechanism of eukaryotic enzyme is well studied, the mechanism of the bacterial enzymes is not well understood. The eukaryotic DPM1 is the catalytic subunit of eukaryotic Dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. The enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133030 [Multi-domain]  Cd Length: 181  Bit Score: 45.93  E-value: 5.86e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGAD-FAPLFAIAQSNYACHLQRHADNKGKGRALKTAISYtldnfpnAKG--IVTIDSDGQHTYEDmmkcLAE 77
Cdd:cd04187    32 IIFVDDGSTDRtLEILRELAARDPRVKVIRLSRNFGQQAALLAGLDH-------ARGdaVITMDADLQDPPEL----IPE 100

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1091089802  78 FLQHPDS---LVLGVRTFEKSVPLRSKFGNLLTRdVLGAMTGVNVSDTQTGLRVI 129
Cdd:cd04187   101 MLAKWEEgydVVYGVRKNRKESWLKRLTSKLFYR-LINKLSGVDIPDNGGDFRLM 154
BcsA COG1215
Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1, ...
 1-83 1.17e-04

Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility];


Pssm-ID: 440828 [Multi-domain]  Cd Length: 303  Bit Score: 43.19  E-value: 1.17e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGADFAPLF-AIAQSNYACHLQRHADNKGKGRALKTAISYtldnfpnAKG--IVTIDSDGQHTyEDMMKCLAE 77
Cdd:COG1215    63 VIVVDDGSTDETAEIArELAAEYPRVRVIERPENGGKAAALNAGLKA-------ARGdiVVFLDADTVLD-PDWLRRLVA 134


                  ....*.
gi 1091089802  78 FLQHPD 83
Cdd:COG1215   135 AFADPG 140
GtrA COG2246
Putative flippase GtrA (transmembrane translocase of bactoprenol-linked glucose) [Lipid ...
 184-252 1.94e-04

Putative flippase GtrA (transmembrane translocase of bactoprenol-linked glucose) [Lipid transport and metabolism];


Pssm-ID: 441847  Cd Length: 133  Bit Score: 40.57  E-value: 1.94e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1091089802 184 RDSIRIYKVFFKYIFSSLFSFLVDIVAFTILISLFnGLSFsavTLASILARVISSVVNFTLNRKVVFKR 252
Cdd:COG2246    66 RGGRSRLREFLRFLLVSLVGLALNLGLLALLVYVL-GLPY---LLAKLIGIVVGTVFNFLLSRRWVFRK 130
GlcNAc-1-P_transferase cd06436
N-acetyl-glucosamine transferase is involved in the synthesis of Poly-beta-1, ...
 1-108 3.08e-04

N-acetyl-glucosamine transferase is involved in the synthesis of Poly-beta-1,6-N-acetyl-D-glucosamine; N-acetyl-glucosamine transferase is responsible for the synthesis of bacteria Poly-beta-1,6-N-acetyl-D-glucosamine (PGA). Poly-beta-1,6-N-acetyl-D-glucosamine is a homopolymer that serves as an adhesion for the maintenance of biofilm structural stability in diverse eubacteria. N-acetyl-glucosamine transferase is the product of gene pgaC. Genetic analysis indicated that all four genes of the pgaABCD locus were required for the PGA production, pgaC being a glycosyltransferase.


Pssm-ID: 133058 [Multi-domain]  Cd Length: 191  Bit Score: 41.22  E-value: 3.08e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGADFAPLFAIAQSNYACH-LQRHADN--KGKGRALKTAISY-----TLDNFPNAKGIVT-IDSDGQ---HTY 68
Cdd:cd06436    28 VLVIDDASDDDTAGIVRLAITDSRVHlLRRHLPNarTGKGDALNAAYDQirqilIEEGADPERVIIAvIDADGRldpNAL 107

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1091089802  69 EDMMKCLAeflqhpDSLVLGVRTfekSVPLRSKFGNLLTR 108
Cdd:cd06436   108 EAVAPYFS------DPRVAGTQS---RVRMYNRHKNLLTI 138
GtrA pfam04138
GtrA-like protein; Members of this family are predicted to be integral membrane proteins with ...
 193-250 4.35e-04

GtrA-like protein; Members of this family are predicted to be integral membrane proteins with three or four transmembrane spans. They are involved in the synthesis of cell surface polysaccharides. The GtrA family are a subset of this family. GtrA is predicted to be an integral membrane protein with 4 transmembrane spans. It is involved is in O antigen modification by Shigella flexneri bacteriophage X (SfX), but does not determine the specificity of glucosylation. Its function remains unknown, but it may play a role in translocation of undecaprenyl phosphate linked glucose (UndP-Glc) across the cytoplasmic membrane. Another member of this family is a DTDP-glucose-4-keto-6-deoxy-D-glucose reductase, which catalyzes the conversion of dTDP-4-keto-6-deoxy-D-glucose to dTDP-D-fucose, which is involved in the biosynthesis of the serotype-specific polysaccharide antigen of Actinobacillus actinomycetemcomitans Y4 (serotype b). This family also includes the teichoic acid glycosylation protein, GtcA, which is a serotype-specific protein in some Listeria innocua and monocytogenes strains. Its exact function is not known, but it is essential for decoration of cell wall teichoic acids with glucose and galactose.


Pssm-ID: 427738  Cd Length: 116  Bit Score: 39.45  E-value: 4.35e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1091089802 193 FFKYIFSSLFSFLVDIVAFTILISLFNglsfSAVTLASILARVISSVVNFTLNRKVVF 250
Cdd:pfam04138  63 LLRFFLVSLAGLLLNLLLLWLLVDGLG----LPPLLAKLVGIAVGTVVNFLLSRFFVF 116
GT_2_like_e cd04192
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 1-105 2.82e-03

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133035 [Multi-domain]  Cd Length: 229  Bit Score: 38.42  E-value: 2.82e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGADFAPL--FAIAQSNYacHLQ----RHADNKGKGRALKTAISytldnfpNAKG--IVTIDSDGQHTyEDMM 72
Cdd:cd04192    31 VILVDDHSTDGTVQIleFAAAKPNF--QLKilnnSRVSISGKKNALTTAIK-------AAKGdwIVTTDADCVVP-SNWL 100

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1091089802  73 KCL-AEFLQHPDSLVLGVRTFEKSVPLRSKFGNL 105
Cdd:cd04192   101 LTFvAFIQKEQIGLVAGPVIYFKGKSLLAKFQRL 134
CESA_like cd06423
CESA_like is the cellulose synthase superfamily; The cellulose synthase (CESA) superfamily ...
 1-83 4.26e-03

CESA_like is the cellulose synthase superfamily; The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.


Pssm-ID: 133045 [Multi-domain]  Cd Length: 180  Bit Score: 37.59  E-value: 4.26e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1091089802   1 IILVDDGSGADFAPLFAIAQSNYAC--HLQRHADNKGKGRALKTAISYtldnfpnAKG--IVTIDSDGqHTYEDMMK-CL 75
Cdd:cd06423    29 VIVVDDGSTDDTLEILEELAALYIRrvLVVRDKENGGKAGALNAGLRH-------AKGdiVVVLDADT-ILEPDALKrLV 100


                  ....*...
gi 1091089802  76 AEFLQHPD 83
Cdd:cd06423   101 VPFFADPK 108
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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