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Conserved domains on  [gi|1085021513|gb|OGS28389|]
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divalent metal ion transporter [Elusimicrobia bacterium RIFCSPHIGHO2_02_FULL_61_10]

Protein Classification

magnesium transporter CorA family protein( domain architecture ID 10191291)

magnesium transporter CorA family protein belonging to the CorA metal ion transporter (MIT) superfamily, similar to CorA which mediates both influx and efflux of magnesium, and may also transport nickel and cobalt

CATH:  3.30.460.20|1.20.58.340
Gene Ontology:  GO:0046873|GO:0030001
PubMed:  17619822|23046658|16179994
SCOP:  4000404|3000358

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EcCorA_ZntB-like_u2 cd12827
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 23-304 2.84e-65

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes.The EcCorA-ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


:

Pssm-ID: 213361 [Multi-domain]  Cd Length: 289  Bit Score: 206.59  E-value: 2.84e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  23 WV-VVSPTEDEKKWLTENFALDEHDFASAFDPEEPARMEFEPD--HLALIFKRPKNYSSkDHFMFKVSTMGLFLFKDKLI 99
Cdd:cd12827     1 WInVVNPTEEEIEQLAEKYGIPEDFLTYALDEEERSRIEYDDDtdYLLIILDVPVEEEE-DDDEYETIPLGIILTKDRLI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 100 LALSEDINMFS------GKYFKQVSgVREVFLKLIYNSIFHFMEHLKVINMIAEEIETKITHSMENRHLLNLFTLEKSLV 173
Cdd:cd12827    80 TICNHETDLLDdflegrVKNFDTSS-KTRFLLQILYRIATSYLKYLRQINKERDELEQELRKSTKNKELFQLLDLEKSLV 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 174 YYLNAINANSYVIDRLKhNTEKVGLSQKSVEFLDDIIIENNQCYRQAEIYSNIFSSLVGARASIVSNNLNILMKNLNAIV 253
Cdd:cd12827   159 YFSTSLKSNEIVLEKLK-RSRSLKLDEEDKELLEDVIIENKQAIEMAEIYSDILSGLMDAFASVISNNLNNVMKFLTSIT 237

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1085021513 254 IAVAIPSFFAGVGGMSElyAITGITNPRVAYPVFMAAMIIIGISTFILIKR 304
Cdd:cd12827   238 IVLTIPTIITGFYGMNV--PLPFADSPYAFWIILLISLLLSVIVALILRRK 286
 
Name Accession Description Interval E-value
EcCorA_ZntB-like_u2 cd12827
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 23-304 2.84e-65

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes.The EcCorA-ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213361 [Multi-domain]  Cd Length: 289  Bit Score: 206.59  E-value: 2.84e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  23 WV-VVSPTEDEKKWLTENFALDEHDFASAFDPEEPARMEFEPD--HLALIFKRPKNYSSkDHFMFKVSTMGLFLFKDKLI 99
Cdd:cd12827     1 WInVVNPTEEEIEQLAEKYGIPEDFLTYALDEEERSRIEYDDDtdYLLIILDVPVEEEE-DDDEYETIPLGIILTKDRLI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 100 LALSEDINMFS------GKYFKQVSgVREVFLKLIYNSIFHFMEHLKVINMIAEEIETKITHSMENRHLLNLFTLEKSLV 173
Cdd:cd12827    80 TICNHETDLLDdflegrVKNFDTSS-KTRFLLQILYRIATSYLKYLRQINKERDELEQELRKSTKNKELFQLLDLEKSLV 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 174 YYLNAINANSYVIDRLKhNTEKVGLSQKSVEFLDDIIIENNQCYRQAEIYSNIFSSLVGARASIVSNNLNILMKNLNAIV 253
Cdd:cd12827   159 YFSTSLKSNEIVLEKLK-RSRSLKLDEEDKELLEDVIIENKQAIEMAEIYSDILSGLMDAFASVISNNLNNVMKFLTSIT 237

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1085021513 254 IAVAIPSFFAGVGGMSElyAITGITNPRVAYPVFMAAMIIIGISTFILIKR 304
Cdd:cd12827   238 IVLTIPTIITGFYGMNV--PLPFADSPYAFWIILLISLLLSVIVALILRRK 286
CorA COG0598
Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];
17-304 8.19e-39

Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];


Pssm-ID: 440363 [Multi-domain]  Cd Length: 305  Bit Score: 138.81  E-value: 8.19e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  17 AEAPALWV-VVSPTEDEKKWLTENFALDEHDFASAFDPEEPARMEFEPDHLALIFKRPKNYSSKDHFmfKVSTMGLFLFK 95
Cdd:COG0598    11 DPEGFVWIdLDGPTDEELEWLAEEFGLHPLALEDALDIEQRPKLEEYDDYLFIVLRTLNYNEEEEEL--ETGPLSIFLGP 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  96 DKLILALSEDINMFSG--KYFKQVSGVR-----EVFLKLIYNSIFHFMEHLKVINMIAEEIETKITHSMENRHLLNLFTL 168
Cdd:COG0598    89 NFLITVRERPLDAFDDvrERLERGPGLLprgpdYLLYALLDAIVDRYFPVLEELEDELDELEDELFESPDNELLRRLYRL 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 169 EKSLVYYLNAINANSYVIDRLKhNTEKVGLSQKSVEFLDDIIIENNQCYRQAEIYSNIFSSLVGARASIVSNNLNILMKN 248
Cdd:COG0598   169 RRELVRLRRALAPLREVLSRLL-RGESPLISEEDREYLRDVYDHLERLLEMVDSLRERLSGLLDAYLSLISNRQNEIMKV 247

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1085021513 249 LNAIVIAVAIPSFFAGVGGMS-----ELyaitgitNPRVAYPVFMAAMIIIGISTFILIKR 304
Cdd:COG0598   248 LTIIAAIFLPPTFIAGIYGMNfegmpEL-------DWPYGYPIVLGLMLLIAVLLYLYFKR 301
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 22-304 2.21e-22

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 94.30  E-value: 2.21e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  22 LWV-VVSPTEDEKKWLTENFALDEHDFASAFDPEEPARMEFEPDHLALIFK--RPKNYSSKDHFmfkvSTMGLFLFKDKL 98
Cdd:pfam01544   5 VWLdLDSPTEAELEWLARTLGLDPLALEDLLDPEERPRVEDYDDYFLLVLPgvNLDDSEEADEL----VTVSILLGDNFL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  99 ILALSEDIN---------MFSGKYFKQVSGVREVFLKLIYNSIFHFMEHlkvINMIAEEIETKITHSMENRHLLNLFTLE 169
Cdd:pfam01544  81 ITVRHEPLPaldevrkrlTLLGGGPLDPGDLLYRLLDEIVDRFLELLEK---LEDELDELEDELEDETNNELLRELAALR 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 170 KSLVYYLNAINANSYVIDRLKHNTEKVGLSQKSVEFLDDIIIENNQCYRQAEIYSNIFSSLVGARASIVSNNLNILMKNL 249
Cdd:pfam01544 158 RSLVYLRRSLLPQRDVLNRLLRREDDPLLDDEQKEYLRDLLDRLERLLEDLDALRERLRSLQDEYSSLLSERTNRVMKVL 237

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1085021513 250 nAIVIAVAIP-SFFAGVGGMSeLYAITGITNPRVAYPVFM-AAMIIIGISTFILIKR 304
Cdd:pfam01544 238 -TVVTAIFLPlTLITGIYGMN-FGGLPELEWSYGYPFVLIlGLMILLAILLLFYFRR 292
 
Name Accession Description Interval E-value
EcCorA_ZntB-like_u2 cd12827
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 23-304 2.84e-65

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes.The EcCorA-ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213361 [Multi-domain]  Cd Length: 289  Bit Score: 206.59  E-value: 2.84e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  23 WV-VVSPTEDEKKWLTENFALDEHDFASAFDPEEPARMEFEPD--HLALIFKRPKNYSSkDHFMFKVSTMGLFLFKDKLI 99
Cdd:cd12827     1 WInVVNPTEEEIEQLAEKYGIPEDFLTYALDEEERSRIEYDDDtdYLLIILDVPVEEEE-DDDEYETIPLGIILTKDRLI 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 100 LALSEDINMFS------GKYFKQVSgVREVFLKLIYNSIFHFMEHLKVINMIAEEIETKITHSMENRHLLNLFTLEKSLV 173
Cdd:cd12827    80 TICNHETDLLDdflegrVKNFDTSS-KTRFLLQILYRIATSYLKYLRQINKERDELEQELRKSTKNKELFQLLDLEKSLV 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 174 YYLNAINANSYVIDRLKhNTEKVGLSQKSVEFLDDIIIENNQCYRQAEIYSNIFSSLVGARASIVSNNLNILMKNLNAIV 253
Cdd:cd12827   159 YFSTSLKSNEIVLEKLK-RSRSLKLDEEDKELLEDVIIENKQAIEMAEIYSDILSGLMDAFASVISNNLNNVMKFLTSIT 237

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1085021513 254 IAVAIPSFFAGVGGMSElyAITGITNPRVAYPVFMAAMIIIGISTFILIKR 304
Cdd:cd12827   238 IVLTIPTIITGFYGMNV--PLPFADSPYAFWIILLISLLLSVIVALILRRK 286
CorA COG0598
Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];
17-304 8.19e-39

Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];


Pssm-ID: 440363 [Multi-domain]  Cd Length: 305  Bit Score: 138.81  E-value: 8.19e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  17 AEAPALWV-VVSPTEDEKKWLTENFALDEHDFASAFDPEEPARMEFEPDHLALIFKRPKNYSSKDHFmfKVSTMGLFLFK 95
Cdd:COG0598    11 DPEGFVWIdLDGPTDEELEWLAEEFGLHPLALEDALDIEQRPKLEEYDDYLFIVLRTLNYNEEEEEL--ETGPLSIFLGP 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  96 DKLILALSEDINMFSG--KYFKQVSGVR-----EVFLKLIYNSIFHFMEHLKVINMIAEEIETKITHSMENRHLLNLFTL 168
Cdd:COG0598    89 NFLITVRERPLDAFDDvrERLERGPGLLprgpdYLLYALLDAIVDRYFPVLEELEDELDELEDELFESPDNELLRRLYRL 168

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 169 EKSLVYYLNAINANSYVIDRLKhNTEKVGLSQKSVEFLDDIIIENNQCYRQAEIYSNIFSSLVGARASIVSNNLNILMKN 248
Cdd:COG0598   169 RRELVRLRRALAPLREVLSRLL-RGESPLISEEDREYLRDVYDHLERLLEMVDSLRERLSGLLDAYLSLISNRQNEIMKV 247

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1085021513 249 LNAIVIAVAIPSFFAGVGGMS-----ELyaitgitNPRVAYPVFMAAMIIIGISTFILIKR 304
Cdd:COG0598   248 LTIIAAIFLPPTFIAGIYGMNfegmpEL-------DWPYGYPIVLGLMLLIAVLLYLYFKR 301
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 22-304 2.21e-22

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 94.30  E-value: 2.21e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  22 LWV-VVSPTEDEKKWLTENFALDEHDFASAFDPEEPARMEFEPDHLALIFK--RPKNYSSKDHFmfkvSTMGLFLFKDKL 98
Cdd:pfam01544   5 VWLdLDSPTEAELEWLARTLGLDPLALEDLLDPEERPRVEDYDDYFLLVLPgvNLDDSEEADEL----VTVSILLGDNFL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  99 ILALSEDIN---------MFSGKYFKQVSGVREVFLKLIYNSIFHFMEHlkvINMIAEEIETKITHSMENRHLLNLFTLE 169
Cdd:pfam01544  81 ITVRHEPLPaldevrkrlTLLGGGPLDPGDLLYRLLDEIVDRFLELLEK---LEDELDELEDELEDETNNELLRELAALR 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 170 KSLVYYLNAINANSYVIDRLKHNTEKVGLSQKSVEFLDDIIIENNQCYRQAEIYSNIFSSLVGARASIVSNNLNILMKNL 249
Cdd:pfam01544 158 RSLVYLRRSLLPQRDVLNRLLRREDDPLLDDEQKEYLRDLLDRLERLLEDLDALRERLRSLQDEYSSLLSERTNRVMKVL 237

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1085021513 250 nAIVIAVAIP-SFFAGVGGMSeLYAITGITNPRVAYPVFM-AAMIIIGISTFILIKR 304
Cdd:pfam01544 238 -TVVTAIFLPlTLITGIYGMN-FGGLPELEWSYGYPFVLIlGLMILLAILLLFYFRR 292
TmCorA-like cd12822
Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential ...
 23-304 1.06e-19

Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the Thermotoga maritima CorA_like family are found in all three kingdoms of life. It is a functionally diverse family, in addition to the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, it includes three Saccharomyces cerevisiae members: two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213356 [Multi-domain]  Cd Length: 289  Bit Score: 86.85  E-value: 1.06e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  23 WV-VVSPTEDEKKWLTENFALDEHDFASAFDPEEPARMEFEPDHLALIFKRPKnySSKDHFMFKVSTMGLFLFKDKLIL- 100
Cdd:cd12822     1 WIdLEGPTEEELQLLAEEFGFHPLAIEDALNERQRPKIEEYDDYLFLVLRALR--YDEEELELETSELSIFLGKNFLVTv 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 101 ------ALSEDINMFSGKYFKQVSGVREVFLKLIYNSIFHFMEHLKVINMIAEEIETKITHSMENRHLLNLFTLEKSLVY 174
Cdd:cd12822    79 hegplrLIDEVIERLLSDPKLLPKGPDFLLYAILDAIVDSYFPILDELEDRLDELEDEILEEPRREVLRELFALRRELLR 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 175 YLNAINANSYVIDRLKHNtEKVGLSQKSVEFLDDIIIENNQCYRQAEIYSNIFSSLVGARASIVSNNLNILMKNLnAIVI 254
Cdd:cd12822   159 LRRILSPQREVLNRLLRR-ENELISEELRPYFRDVYDHLERLAELLDSYRDLLSSLLDAYLSLQSNRTNEVMKVL-TIIT 236

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1085021513 255 AVAIP-SFFAGVGGMS-----ELyaitgitNPRVAYPVFMAAMIIIGISTFILIKR 304
Cdd:cd12822   237 TIFLPlTFITGIYGMNfeympEL-------HWPYGYFIVLGIMLLIAGGLLWWFRR 285
EcCorA_ZntB-like cd12821
Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily ...
 25-304 2.42e-11

Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of this family are found in all three kingdoms of life. It is a functionally diverse family, including the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs (which can also transport Co2+, and Ni2+ ), and the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). It also includes two Saccharomyces cerevisiae members: the inner membrane Mg2+ transporters Mfm1p/Lpe10p, and Mrs2p, and a family of Arabidopsis thaliana members (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213355 [Multi-domain]  Cd Length: 285  Bit Score: 63.11  E-value: 2.42e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  25 VVSPTEDEKKWLTENFALDEHDFASAFDPEEPARmEFEPDHLALIFKRpknySSKDHFMFKVSTMGLFLFKDKLIL---- 100
Cdd:cd12821     4 IDEPDDEIRRLLEELESSHLPNDLRDILLGVSFP-RFEEVSGSGIYIQ----LRKPQKDADTASFHFLITRDRLITvrld 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 101 ---ALSEDINMFSGKYFKQVSGVREVFLKLIYNSIFHFMEHLKVINMIAEEIETKITHSMENRHLLNLFTLEKSLVYYLN 177
Cdd:cd12821    79 elpALDELRQDLRSGQMKSAKSAAELLFVLLGAIIKALLTGIDQFEEKLEELEWDLLEGNNAIKLDRILELRRELLRLTN 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 178 AINANSYVIDRLKHNTEKVGLSQKSVEFlDDIIIENNQCYRQAEIYSNIFSSLVGARASIVSNNLNILMKNLnAIVIAVA 257
Cdd:cd12821   159 LIEPQQEVLMALQEAFAELLFSEDEEEL-RRTLDRIERLLQLIEEYEQELDTLQDIEEVVSSHRGNEIMKTL-TVVTTVF 236

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1085021513 258 IP-SFFAG-----VGGMSELyaitgitNPRVAYPVFMAAMIIIGISTFILIKR 304
Cdd:cd12821   237 LPlTLIGGlwgmnFKNMPEL-------EWKYGYLFVLGLIILSTAGLYLYLRR 282
TmCorA-like_1 cd12828
Thermotoga maritima CorA_like subfamily; This subfamily belongs to the Thermotoga maritima ...
 147-304 2.98e-05

Thermotoga maritima CorA_like subfamily; This subfamily belongs to the Thermotoga maritima CorA (TmCorA)-family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of this subfamily are found in all three kingdoms of life. It is functionally diverse subfamily, in addition to the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, it includes Methanosarcina mazei CorA which may be involved in transport of copper and/or other divalent metal ions. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by a related protein, Saccharomyces cerevisiae Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213362 [Multi-domain]  Cd Length: 294  Bit Score: 44.73  E-value: 2.98e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 147 EEIETKITHSMENRHLLNLFTLEKSLVYYLNAINANSYVIDRLKHNtEKVGLSQKSVEFLDDI------IIENnqcyrqA 220
Cdd:cd12828   136 EDLEEELLENPTPETLERIHKLKRELLFLRRSIWPLREVINRLLRG-ESPLISEETRPYLRDVydhtiqVIDT------I 208

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 221 EIYSNIFSSLVGARASIVSNNLNILMKNLnAIVIAVAIP-SFFAGVGGMS-----ELYAitgitnpRVAYPVFMAAMIII 294
Cdd:cd12828   209 ETYRDMLSGLLDLYLSSVSNRMNEVMKVL-TIISTIFIPlTFIAGIYGMNfeympELEW-------KYGYPAVLGVMLLI 280

                         170
                  ....*....|
gi 1085021513 295 GISTFILIKR 304
Cdd:cd12828   281 ALGMLLYFKR 290
TmCorA-like_u3 cd12832
Uncharacterized subfamily of the Thermotoga maritima CorA-like family; This subfamily belongs ...
 23-304 5.99e-04

Uncharacterized subfamily of the Thermotoga maritima CorA-like family; This subfamily belongs to the Thermotoga maritima CorA (TmCorA)-like family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the TmCorA-like family are found in all three kingdoms of life. It is a functionally diverse family which includes the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, and three Saccharomyces cerevisiae proteins: two located in the plasma membrane: the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by a related protein, Saccharomyces cerevisiae Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213366 [Multi-domain]  Cd Length: 287  Bit Score: 40.66  E-value: 5.99e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513  23 WV-VVSPTEDEKKWLTENFALDEHDFASAFDPEEPARMEFEPDHLALIFKRPKNYSSK-DHFMFKVSTMGLFLFKDKLIL 100
Cdd:cd12832     1 WIdVVAPTEEDLEDLARRYNLHETSLKDCLDPDHLPKYEEVDGVTFIILRVYTDKKREnADTIQELTTKIAIFVVDKFVI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 101 -----------ALSEDINMFSGKYfkqvSGVREVFLKLIYNSIFHFMEHLKVINMIAEEIETKITHSMENRHLL-NLFTL 168
Cdd:cd12832    81 tihrtdlefiaNLKEKWLSGMHKC----ESPTELLLRIVKDALKTYEAPLEKLSEELDKYEEEVFLKKRKGSLLkRLYHL 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1085021513 169 EKSLVYYLNAINANSYVIDRLKHNTEKVGLSQKSVEFLDDIiiennqCYRQAEIYSNIfSSLVGARASIVSNNLNILMKN 248
Cdd:cd12832   157 KRKASVIKRVLNLTQDVIDKIDTPLKSDPYTQDLRDLLIKL------LSLYDELHENA-TNLLSLYISLSSQRTNEVMRV 229

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1085021513 249 LnAIVIAVAIP-SFFAGVGGMS-----ELYAITGitnprvaYPVFMAAMIIIGISTFILIKR 304
Cdd:cd12832   230 L-TIFSVFFLPlTFIAGVYGMNfkympELHWKYG-------YPATLLVMIVIVVGIYFWFKR 283
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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