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Conserved domains on  [gi|1080759744|gb|OFP28399|]
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alkaline-shock protein, partial [Staphylococcus sp. HMSC057C08]

Protein Classification

Asp23/Gls24 family envelope stress response protein( domain architecture ID 10003467)

Asp23/Gls24 family envelope stress response protein similar to Staphylococcus aureus alkaline shock protein 23 (Asp23) and Streptococcus pyogenes stress response regulator gls24 homolog

PubMed:  7864904

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
YloU COG1302
Uncharacterized conserved protein YloU, alkaline shock protein (Asp23) family [Function ...
 39-150 1.53e-36

Uncharacterized conserved protein YloU, alkaline shock protein (Asp23) family [Function unknown];


:

Pssm-ID: 440913  Cd Length: 118  Bit Score: 121.81  E-value: 1.53e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1080759744  39 KLTFADEVIEKISGIAAREVNGILSLKGNFVDSISNQFsNTENVTQGVSVEVGEKQTAVDLKVVLEYGESAPKIFNKVTD 118
Cdd:COG1302     8 KITIADEVIAKIAGIAALEVYGVVGMAGGFADGIAELL-GRKNLTKGVKVEVGEDQVAIDLYVVVEYGVNIPEVAENVQE 86

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1080759744 119 LIKEQVKYMTGLQVVEVNMRVEDVMTRKEFEQ 150
Cdd:COG1302    87 NVKYAVEKMTGLEVVEVNVHVQGVRFPEEEEE 118
 
Name Accession Description Interval E-value
YloU COG1302
Uncharacterized conserved protein YloU, alkaline shock protein (Asp23) family [Function ...
 39-150 1.53e-36

Uncharacterized conserved protein YloU, alkaline shock protein (Asp23) family [Function unknown];


Pssm-ID: 440913  Cd Length: 118  Bit Score: 121.81  E-value: 1.53e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1080759744  39 KLTFADEVIEKISGIAAREVNGILSLKGNFVDSISNQFsNTENVTQGVSVEVGEKQTAVDLKVVLEYGESAPKIFNKVTD 118
Cdd:COG1302     8 KITIADEVIAKIAGIAALEVYGVVGMAGGFADGIAELL-GRKNLTKGVKVEVGEDQVAIDLYVVVEYGVNIPEVAENVQE 86

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1080759744 119 LIKEQVKYMTGLQVVEVNMRVEDVMTRKEFEQ 150
Cdd:COG1302    87 NVKYAVEKMTGLEVVEVNVHVQGVRFPEEEEE 118
Asp23 pfam03780
Asp23 family, cell envelope-related function; The alkaline shock protein Asp23 was identified ...
 39-143 1.29e-33

Asp23 family, cell envelope-related function; The alkaline shock protein Asp23 was identified as an alkaline shock protein that was expressed in a sigmaB-dependent manner in Staphylococcus aureus. Following an alkaline shock Asp23 accumulates in the soluble protein fraction of the S. aureus cell. Asp23 is one of the most abundant proteins in the cytosolic protein fraction of stationary S. aureus cells, with a copy-number of >25000 per cell. A second Asp23-family protein, AmaP, which is encoded within the asp23-operon, is required to localize Asp23 to the cell membrane. The overall function for the family is thus a cell envelope-related one in Gram-positive bacteria. UniProtKB:Q2FW64.


Pssm-ID: 427500  Cd Length: 108  Bit Score: 114.15  E-value: 1.29e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1080759744  39 KLTFADEVIEKISGIAAREVNGILSLKGNFVDSISNQFSNtENVTQGVSVEVGE-KQTAVDLKVVLEYGESAPKIFNKVT 117
Cdd:pfam03780   4 EITIADEVIAKIAGIAATEVDGVVGMAGGLKDGLAERLGR-ENLTKGVKVEVGEeGQVAVDLYVIVEYGVSIPEVAENIQ 82

                          90       100
                  ....*....|....*....|....*.
gi 1080759744 118 DLIKEQVKYMTGLQVVEVNMRVEDVM 143
Cdd:pfam03780  83 ENVKYAVETMTGLEVVEVNVHVQGVR 108
anchor_AmaP NF033218
alkaline shock response membrane anchor protein AmaP; The founding member of this family, AmaP ...
 67-139 4.50e-03

alkaline shock response membrane anchor protein AmaP; The founding member of this family, AmaP (Asp23 membrane anchoring protein), is related to Asp23 through part of its length, but includes a highly hydrophobic N-terminal region that should make it an integral membrane protein. Asp23 (alkaline shock protein of 23 kDa), described in PMID:7864904, is a cytosolic protein in Staphylococcus aureus, strongly induced by a pH shift from 7 to 10, and also recruited to the membrane. AmaP appears to be the partner protein with an integral membrane segment and the ability to anchor Asp23 to the membrane. This model was built to identify full-length homologs of AmaP, while excluding Asp23. Some but not all members of this family score above the cutoffs of Pfam model version PF03780.11, but full-length homologs of Asp23 score considerably higher. Asp23 family members previously were known as DUF322.


Pssm-ID: 468002  Cd Length: 166  Bit Score: 35.64  E-value: 4.50e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1080759744  67 NFVDSISNQFSNTENVTqgVSVEVGEKQTAVDLKVVLEYGESAPKIFNKVTDLIKEQVKYMTGLQVVEVNMRV 139
Cdd:NF033218   96 GFVREALSKVDGVRNPK--VKVKLKKRKIKVDVKGDLDPRSNLPELTEEIQEEIKEGLEELLGIPVKKVKVKV 166
 
Name Accession Description Interval E-value
YloU COG1302
Uncharacterized conserved protein YloU, alkaline shock protein (Asp23) family [Function ...
 39-150 1.53e-36

Uncharacterized conserved protein YloU, alkaline shock protein (Asp23) family [Function unknown];


Pssm-ID: 440913  Cd Length: 118  Bit Score: 121.81  E-value: 1.53e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1080759744  39 KLTFADEVIEKISGIAAREVNGILSLKGNFVDSISNQFsNTENVTQGVSVEVGEKQTAVDLKVVLEYGESAPKIFNKVTD 118
Cdd:COG1302     8 KITIADEVIAKIAGIAALEVYGVVGMAGGFADGIAELL-GRKNLTKGVKVEVGEDQVAIDLYVVVEYGVNIPEVAENVQE 86

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1080759744 119 LIKEQVKYMTGLQVVEVNMRVEDVMTRKEFEQ 150
Cdd:COG1302    87 NVKYAVEKMTGLEVVEVNVHVQGVRFPEEEEE 118
Asp23 pfam03780
Asp23 family, cell envelope-related function; The alkaline shock protein Asp23 was identified ...
 39-143 1.29e-33

Asp23 family, cell envelope-related function; The alkaline shock protein Asp23 was identified as an alkaline shock protein that was expressed in a sigmaB-dependent manner in Staphylococcus aureus. Following an alkaline shock Asp23 accumulates in the soluble protein fraction of the S. aureus cell. Asp23 is one of the most abundant proteins in the cytosolic protein fraction of stationary S. aureus cells, with a copy-number of >25000 per cell. A second Asp23-family protein, AmaP, which is encoded within the asp23-operon, is required to localize Asp23 to the cell membrane. The overall function for the family is thus a cell envelope-related one in Gram-positive bacteria. UniProtKB:Q2FW64.


Pssm-ID: 427500  Cd Length: 108  Bit Score: 114.15  E-value: 1.29e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1080759744  39 KLTFADEVIEKISGIAAREVNGILSLKGNFVDSISNQFSNtENVTQGVSVEVGE-KQTAVDLKVVLEYGESAPKIFNKVT 117
Cdd:pfam03780   4 EITIADEVIAKIAGIAATEVDGVVGMAGGLKDGLAERLGR-ENLTKGVKVEVGEeGQVAVDLYVIVEYGVSIPEVAENIQ 82

                          90       100
                  ....*....|....*....|....*.
gi 1080759744 118 DLIKEQVKYMTGLQVVEVNMRVEDVM 143
Cdd:pfam03780  83 ENVKYAVETMTGLEVVEVNVHVQGVR 108
anchor_AmaP NF033218
alkaline shock response membrane anchor protein AmaP; The founding member of this family, AmaP ...
 67-139 4.50e-03

alkaline shock response membrane anchor protein AmaP; The founding member of this family, AmaP (Asp23 membrane anchoring protein), is related to Asp23 through part of its length, but includes a highly hydrophobic N-terminal region that should make it an integral membrane protein. Asp23 (alkaline shock protein of 23 kDa), described in PMID:7864904, is a cytosolic protein in Staphylococcus aureus, strongly induced by a pH shift from 7 to 10, and also recruited to the membrane. AmaP appears to be the partner protein with an integral membrane segment and the ability to anchor Asp23 to the membrane. This model was built to identify full-length homologs of AmaP, while excluding Asp23. Some but not all members of this family score above the cutoffs of Pfam model version PF03780.11, but full-length homologs of Asp23 score considerably higher. Asp23 family members previously were known as DUF322.


Pssm-ID: 468002  Cd Length: 166  Bit Score: 35.64  E-value: 4.50e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1080759744  67 NFVDSISNQFSNTENVTqgVSVEVGEKQTAVDLKVVLEYGESAPKIFNKVTDLIKEQVKYMTGLQVVEVNMRV 139
Cdd:NF033218   96 GFVREALSKVDGVRNPK--VKVKLKKRKIKVDVKGDLDPRSNLPELTEEIQEEIKEGLEELLGIPVKKVKVKV 166
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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