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Conserved domains on  [gi|1072746991|gb|AOT25489|]
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helix-turn-helix DNA binding domain protein [Mycobacterium phage BabyRay]

Protein Classification

helix-turn-helix domain-containing protein( domain architecture ID 229396)

helix-turn-helix domain-containing protein may bind DNA and be involved in the regulation of gene expression

Gene Ontology:  GO:0003677|GO:0006355

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
HTH_CRP super family cl46859
helix_turn_helix, cAMP Regulatory protein C-terminus; DNA binding domain of prokaryotic ...
42-85 9.11e-08

helix_turn_helix, cAMP Regulatory protein C-terminus; DNA binding domain of prokaryotic regulatory proteins belonging to the catabolite activator protein family.


The actual alignment was detected with superfamily member cd00569:

Pssm-ID: 481199 [Multi-domain]  Cd Length: 42  Bit Score: 44.24  E-value: 9.11e-08
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 1072746991 42 GRPnrKKLTPEEAKYIREMARSGIPQAEIAWSFDVNRSTISRIV 85
Cdd:cd00569    1 GRP--PKLTPEQIAEARRLLAAGESVAEIARELGVSRSTLYRYL 42
 
Name Accession Description Interval E-value
HTH_Hin_like cd00569
Helix-turn-helix domain of Hin and related proteins; This domain model summarizes a family of ...
42-85 9.11e-08

Helix-turn-helix domain of Hin and related proteins; This domain model summarizes a family of DNA-binding domains unique to bacteria and represented by the Hin protein of Salmonella. The basic HTH domain is a simple fold comprised of three core helices that form a right-handed helical bundle. The principal DNA-protein interface is formed by the third helix, the recognition helix, inserting itself into the major groove of the DNA. A diverse array of HTH domains participate in a variety of functions that depend on their DNA-binding properties. HTH_Hin represents one of the simplest versions of the HTH domains; the characterization of homologous relationships between various sequence-diverse HTH domain families remains difficult. The Hin recombinase induces the site-specific inversion of a chromosomal DNA segment containing a promoter, which controls the alternate expression of two genes by reversibly switching orientation. The Hin recombinase consists of a single polypeptide chain containing a C-terminal DNA-binding domain (HTH_Hin) and a catalytic domain.


Pssm-ID: 259851 [Multi-domain]  Cd Length: 42  Bit Score: 44.24  E-value: 9.11e-08
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 1072746991 42 GRPnrKKLTPEEAKYIREMARSGIPQAEIAWSFDVNRSTISRIV 85
Cdd:cd00569    1 GRP--PKLTPEQIAEARRLLAAGESVAEIARELGVSRSTLYRYL 42
HTH_7 pfam02796
Helix-turn-helix domain of resolvase;
42-84 4.92e-04

Helix-turn-helix domain of resolvase;


Pssm-ID: 397088 [Multi-domain]  Cd Length: 45  Bit Score: 34.64  E-value: 4.92e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 1072746991 42 GRPnrKKLTPEEAKYIREMARSGIPQAEIAWSFDVNRSTISRI 84
Cdd:pfam02796  1 GRP--PKLNEEDINEVITLLEEGISIKQIAKIFGISRSTVYRY 41
Tra8 COG2826
Transposase and inactivated derivatives, IS30 family [Mobilome: prophages, transposons];
43-86 2.31e-03

Transposase and inactivated derivatives, IS30 family [Mobilome: prophages, transposons];


Pssm-ID: 442074 [Multi-domain]  Cd Length: 325  Bit Score: 35.24  E-value: 2.31e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1072746991  43 RPNRKKLTPEEAKYIREMARSGIPQAEIAWSFDVNRSTISRIVR 86
Cdd:COG2826     1 MRSYKHLTLEEREEIEALLKAGLSVREIARRLGRSPSTISRELK 44
 
Name Accession Description Interval E-value
HTH_Hin_like cd00569
Helix-turn-helix domain of Hin and related proteins; This domain model summarizes a family of ...
42-85 9.11e-08

Helix-turn-helix domain of Hin and related proteins; This domain model summarizes a family of DNA-binding domains unique to bacteria and represented by the Hin protein of Salmonella. The basic HTH domain is a simple fold comprised of three core helices that form a right-handed helical bundle. The principal DNA-protein interface is formed by the third helix, the recognition helix, inserting itself into the major groove of the DNA. A diverse array of HTH domains participate in a variety of functions that depend on their DNA-binding properties. HTH_Hin represents one of the simplest versions of the HTH domains; the characterization of homologous relationships between various sequence-diverse HTH domain families remains difficult. The Hin recombinase induces the site-specific inversion of a chromosomal DNA segment containing a promoter, which controls the alternate expression of two genes by reversibly switching orientation. The Hin recombinase consists of a single polypeptide chain containing a C-terminal DNA-binding domain (HTH_Hin) and a catalytic domain.


Pssm-ID: 259851 [Multi-domain]  Cd Length: 42  Bit Score: 44.24  E-value: 9.11e-08
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 1072746991 42 GRPnrKKLTPEEAKYIREMARSGIPQAEIAWSFDVNRSTISRIV 85
Cdd:cd00569    1 GRP--PKLTPEQIAEARRLLAAGESVAEIARELGVSRSTLYRYL 42
HTH_7 pfam02796
Helix-turn-helix domain of resolvase;
42-84 4.92e-04

Helix-turn-helix domain of resolvase;


Pssm-ID: 397088 [Multi-domain]  Cd Length: 45  Bit Score: 34.64  E-value: 4.92e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 1072746991 42 GRPnrKKLTPEEAKYIREMARSGIPQAEIAWSFDVNRSTISRI 84
Cdd:pfam02796  1 GRP--PKLNEEDINEVITLLEEGISIKQIAKIFGISRSTVYRY 41
HTH_38 pfam13936
Helix-turn-helix domain; This helix-turn-helix domain is often found in transferases and is ...
46-86 1.89e-03

Helix-turn-helix domain; This helix-turn-helix domain is often found in transferases and is likely to be DNA-binding.


Pssm-ID: 433591 [Multi-domain]  Cd Length: 44  Bit Score: 33.25  E-value: 1.89e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 1072746991 46 RKKLTPEEAKYIREMARSGIPQAEIAWSFDVNRSTISRIVR 86
Cdd:pfam13936  2 GKHLSLEEREEIARLLAEGLSLREIARRLGRSPSTISRELR 42
Tra8 COG2826
Transposase and inactivated derivatives, IS30 family [Mobilome: prophages, transposons];
43-86 2.31e-03

Transposase and inactivated derivatives, IS30 family [Mobilome: prophages, transposons];


Pssm-ID: 442074 [Multi-domain]  Cd Length: 325  Bit Score: 35.24  E-value: 2.31e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1072746991  43 RPNRKKLTPEEAKYIREMARSGIPQAEIAWSFDVNRSTISRIVR 86
Cdd:COG2826     1 MRSYKHLTLEEREEIEALLKAGLSVREIARRLGRSPSTISRELK 44
HTH_Tnp_4 pfam13613
Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding ...
47-88 9.88e-03

Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding region of transposase enzymes, necessary for efficient DNA transposition. Most of the members derive from the IS superfamily IS5 and rather fewer from IS4.


Pssm-ID: 463934  Cd Length: 53  Bit Score: 31.33  E-value: 9.88e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 1072746991 47 KKLTPEE-----AKYIREmarsGIPQAEIAWSFDVNRSTISRIVRGI 88
Cdd:pfam13613  1 RKLSLEDqllltLVYLRL----NLTFQDLAYRFGVSESTASRIFHKW 43
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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