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Conserved domains on  [gi|1007374374|ref|NP_001308088|]
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tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial isoform 4 [Homo sapiens]

Protein Classification

class I SAM-dependent methyltransferase( domain architecture ID 10614797)

class I SAM-dependent methyltransferase catalyzes the methylation of one or more specific substrates using S-adenosyl-L-methionine (SAM or AdoMet) as the methyl donor

CATH:  2.20.25.110
EC:  2.1.-.-|2.1.1.-
Gene Ontology:  GO:0032259|GO:0008757|GO:0008168|GO:1904047
PubMed:  12504684|12826405

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 150-253 6.16e-14

Methyltransferase domain; This family appears to be a methyltransferase domain.


:

Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 66.82  E-value: 6.16e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 150 ILEVGCGAGNSVFPILNTLENSpesfLYCCDFASGAVELVKSHSSYRATQCFAFVHDVCDdglpYPFPDGILDVILLVFV 229
Cdd:pfam13649   1 VLDLGCGTGRLTLALARRGGAR----VTGVDLSPEMLERARERAAEAGLNVEFVQGDAED----LPFPDGSFDLVVSSGV 72

                          90       100
                  ....*....|....*....|....
gi 1007374374 230 LSSIHPDRMQGVVNRLSKLLKPGG 253
Cdd:pfam13649  73 LHHLPDPDLEAALREIARVLKPGG 96
 
Name Accession Description Interval E-value
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 150-253 6.16e-14

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 66.82  E-value: 6.16e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 150 ILEVGCGAGNSVFPILNTLENSpesfLYCCDFASGAVELVKSHSSYRATQCFAFVHDVCDdglpYPFPDGILDVILLVFV 229
Cdd:pfam13649   1 VLDLGCGTGRLTLALARRGGAR----VTGVDLSPEMLERARERAAEAGLNVEFVQGDAED----LPFPDGSFDLVVSSGV 72

                          90       100
                  ....*....|....*....|....
gi 1007374374 230 LSSIHPDRMQGVVNRLSKLLKPGG 253
Cdd:pfam13649  73 LHHLPDPDLEAALREIARVLKPGG 96
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 149-269 3.08e-13

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 66.17  E-value: 3.08e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 149 RILEVGCGAGNSVFPILNTlenspESFLYCCDFASGAVELVKShssyRATQCFAFVHDVCDDGLPYPFPDGILDVILLVF 228
Cdd:COG2226    25 RVLDLGCGTGRLALALAER-----GARVTGVDISPEMLELARE----RAAEAGLNVEFVVGDAEDLPFPDGSFDLVISSF 95

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1007374374 229 VLSSIhPDRmQGVVNRLSKLLKPGGMLLFRDYGRYDKTQLR 269
Cdd:COG2226    96 VLHHL-PDP-ERALAEIARVLKPGGRLVVVDFSPPDLAELE 134
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 149-257 5.46e-09

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 53.20  E-value: 5.46e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 149 RILEVGCGAGNsvfpILNTLENSPESFLYCCDFASGAVELVKSHSSYRATQCFAFVHdvCDDGLPYPFPDGILDVILLVF 228
Cdd:cd02440     1 RVLDLGCGTGA----LALALASGPGARVTGVDISPVALELARKAAAALLADNVEVLK--GDAEELPPEADESFDVIISDP 74

                          90       100
                  ....*....|....*....|....*....
gi 1007374374 229 VLSSIHPDrMQGVVNRLSKLLKPGGMLLF 257
Cdd:cd02440    75 PLHHLVED-LARFLEEARRLLKPGGVLVL 102
PRK08317 PRK08317
hypothetical protein; Provisional
 140-253 1.07e-06

hypothetical protein; Provisional


Pssm-ID: 181382 [Multi-domain]  Cd Length: 241  Bit Score: 49.16  E-value: 1.07e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 140 LFPGSnatfRILEVGCGAGNSVFPILNtlENSPESFLYCCDFASGAVELVKShssyRATQCFAFVHDVCDDGLPYPFPDG 219
Cdd:PRK08317   17 VQPGD----RVLDVGCGPGNDARELAR--RVGPEGRVVGIDRSEAMLALAKE----RAAGLGPNVEFVRGDADGLPFPDG 86

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1007374374 220 ILDVILLVFVLSsiHPDRMQGVVNRLSKLLKPGG 253
Cdd:PRK08317   87 SFDAVRSDRVLQ--HLEDPARALAEIARVLRPGG 118
 
Name Accession Description Interval E-value
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 150-253 6.16e-14

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 66.82  E-value: 6.16e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 150 ILEVGCGAGNSVFPILNTLENSpesfLYCCDFASGAVELVKSHSSYRATQCFAFVHDVCDdglpYPFPDGILDVILLVFV 229
Cdd:pfam13649   1 VLDLGCGTGRLTLALARRGGAR----VTGVDLSPEMLERARERAAEAGLNVEFVQGDAED----LPFPDGSFDLVVSSGV 72

                          90       100
                  ....*....|....*....|....
gi 1007374374 230 LSSIHPDRMQGVVNRLSKLLKPGG 253
Cdd:pfam13649  73 LHHLPDPDLEAALREIARVLKPGG 96
Methyltransf_12 pfam08242
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 151-255 1.40e-13

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 400515 [Multi-domain]  Cd Length: 98  Bit Score: 65.85  E-value: 1.40e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 151 LEVGCGAGNSVFPILNTLensPESFLYCCDFASGAVELVKSH-SSYRATQCFAFVHDVCDDGLPYPFPdgiLDVILLVFV 229
Cdd:pfam08242   1 LEIGCGTGTLLRALLEAL---PGLEYTGLDISPAALEAARERlAALGLLNAVRVELFQLDLGELDPGS---FDVVVASNV 74

                          90       100
                  ....*....|....*....|....*.
gi 1007374374 230 LSsiHPDRMQGVVNRLSKLLKPGGML 255
Cdd:pfam08242  75 LH--HLADPRAVLRNIRRLLKPGGVL 98
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 149-269 3.08e-13

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 66.17  E-value: 3.08e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 149 RILEVGCGAGNSVFPILNTlenspESFLYCCDFASGAVELVKShssyRATQCFAFVHDVCDDGLPYPFPDGILDVILLVF 228
Cdd:COG2226    25 RVLDLGCGTGRLALALAER-----GARVTGVDISPEMLELARE----RAAEAGLNVEFVVGDAEDLPFPDGSFDLVISSF 95

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1007374374 229 VLSSIhPDRmQGVVNRLSKLLKPGGMLLFRDYGRYDKTQLR 269
Cdd:COG2226    96 VLHHL-PDP-ERALAEIARVLKPGGRLVVVDFSPPDLAELE 134
SmtA COG0500
SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, ...
 140-302 7.73e-11

SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, General function prediction only];


Pssm-ID: 440266 [Multi-domain]  Cd Length: 199  Bit Score: 60.70  E-value: 7.73e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 140 LFPGSNATFRILEVGCGAGNSVFPILNTLEnspeSFLYCCDFASGAVEL-VKSHSSYRATQCFAFVHDVCDdglPYPFPD 218
Cdd:COG0500    20 LLERLPKGGRVLDLGCGTGRNLLALAARFG----GRVIGIDLSPEAIALaRARAAKAGLGNVEFLVADLAE---LDPLPA 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 219 GILDVILLVFVLSSIHPDRMQGVVNRLSKLLKPGGMLLF--------RDYGRYDKTQLRFKKGHCLSENFYVRGDGTRAY 290
Cdd:COG0500    93 ESFDLVVAFGVLHHLPPEEREALLRELARALKPGGVLLLsasdaaaaLSLARLLLLATASLLELLLLLRLLALELYLRAL 172

                         170
                  ....*....|..
gi 1007374374 291 FFTKGEVHSMFC 302
Cdd:COG0500   173 LAAAATEDLRSD 184
Methyltransf_11 pfam08241
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 151-257 5.01e-10

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 462406 [Multi-domain]  Cd Length: 94  Bit Score: 55.75  E-value: 5.01e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 151 LEVGCGAGNSVFPILNTLENspesfLYCCDFASGAVELVKSHSSYRAtqcfafVHDVCDDGLPYPFPDGILDVILLVFVL 230
Cdd:pfam08241   1 LDVGCGTGLLTELLARLGAR-----VTGVDISPEMLELAREKAPREG------LTFVVGDAEDLPFPDNSFDLVLSSEVL 69

                          90       100
                  ....*....|....*....|....*..
gi 1007374374 231 SsiHPDRMQGVVNRLSKLLKPGGMLLF 257
Cdd:pfam08241  70 H--HVEDPERALREIARVLKPGGILII 94
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 149-257 5.46e-09

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 53.20  E-value: 5.46e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 149 RILEVGCGAGNsvfpILNTLENSPESFLYCCDFASGAVELVKSHSSYRATQCFAFVHdvCDDGLPYPFPDGILDVILLVF 228
Cdd:cd02440     1 RVLDLGCGTGA----LALALASGPGARVTGVDISPVALELARKAAAALLADNVEVLK--GDAEELPPEADESFDVIISDP 74

                          90       100
                  ....*....|....*....|....*....
gi 1007374374 229 VLSSIHPDrMQGVVNRLSKLLKPGGMLLF 257
Cdd:cd02440    75 PLHHLVED-LARFLEEARRLLKPGGVLVL 102
Tam COG4106
Trans-aconitate methyltransferase [Energy production and conversion];
149-257 1.45e-08

Trans-aconitate methyltransferase [Energy production and conversion];


Pssm-ID: 443282 [Multi-domain]  Cd Length: 100  Bit Score: 51.75  E-value: 1.45e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 149 RILEVGCGAGNSVFPILntlENSPESFLYCCDFASGAVELVKShssyRATQCFAFVHDVCDDGLPYPFpdgilDVILLVF 228
Cdd:COG4106     4 RVLDLGCGTGRLTALLA---ERFPGARVTGVDLSPEMLARARA----RLPNVRFVVADLRDLDPPEPF-----DLVVSNA 71

                          90       100
                  ....*....|....*....|....*....
gi 1007374374 229 VLSsiHPDRMQGVVNRLSKLLKPGGMLLF 257
Cdd:COG4106    72 ALH--WLPDHAALLARLAAALAPGGVLAV 98
UbiG COG2227
2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase [Coenzyme transport and ...
 149-260 1.56e-08

2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase [Coenzyme transport and metabolism]; 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase is part of the Pathway/BioSystem: Ubiquinone biosynthesis


Pssm-ID: 441829 [Multi-domain]  Cd Length: 126  Bit Score: 52.33  E-value: 1.56e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 149 RILEVGCGAGNSVFPILNTLENspesfLYCCDFASGAVELVKSHSSYRAtqcfafVHDVCDDGLPYPFPDGILDVILLVF 228
Cdd:COG2227    27 RVLDVGCGTGRLALALARRGAD-----VTGVDISPEALEIARERAAELN------VDFVQGDLEDLPLEDGSFDLVICSE 95

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1007374374 229 VLssIHPDRMQGVVNRLSKLLKPGGMLLFRDY 260
Cdd:COG2227    96 VL--EHLPDPAALLRELARLLKPGGLLLLSTP 125
PRK08317 PRK08317
hypothetical protein; Provisional
 140-253 1.07e-06

hypothetical protein; Provisional


Pssm-ID: 181382 [Multi-domain]  Cd Length: 241  Bit Score: 49.16  E-value: 1.07e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 140 LFPGSnatfRILEVGCGAGNSVFPILNtlENSPESFLYCCDFASGAVELVKShssyRATQCFAFVHDVCDDGLPYPFPDG 219
Cdd:PRK08317   17 VQPGD----RVLDVGCGPGNDARELAR--RVGPEGRVVGIDRSEAMLALAKE----RAAGLGPNVEFVRGDADGLPFPDG 86

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1007374374 220 ILDVILLVFVLSsiHPDRMQGVVNRLSKLLKPGG 253
Cdd:PRK08317   87 SFDAVRSDRVLQ--HLEDPARALAEIARVLRPGG 118
COG4976 COG4976
Predicted methyltransferase, contains TPR repeat [General function prediction only];
149-263 3.97e-06

Predicted methyltransferase, contains TPR repeat [General function prediction only];


Pssm-ID: 444001 [Multi-domain]  Cd Length: 181  Bit Score: 46.53  E-value: 3.97e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 149 RILEVGCGAGNSVFPILntlensPESF-LYCCDFASGAVELVKSHSSYRATqcfaFVHDVCDDglpyPFPDGILDVILLV 227
Cdd:COG4976    49 RVLDLGCGTGLLGEALR------PRGYrLTGVDLSEEMLAKAREKGVYDRL----LVADLADL----AEPDGRFDLIVAA 114

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1007374374 228 FVLSsiHPDRMQGVVNRLSKLLKPGGMLLF-----RDYGRY 263
Cdd:COG4976   115 DVLT--YLGDLAAVFAGVARALKPGGLFIFsvedaDGSGRY 153
Methyltransf_23 pfam13489
Methyltransferase domain; This family appears to be a methyltransferase domain.
 140-301 9.83e-05

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 404385 [Multi-domain]  Cd Length: 162  Bit Score: 42.42  E-value: 9.83e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 140 LFPGSNATFRILEVGCGAGNsVFPILNtlENSPESFLYccDFASGAVELVKShssyratqcfaFVHDVCDDGLPYPFPDG 219
Cdd:pfam13489  16 LLPKLPSPGRVLDFGCGTGI-FLRLLR--AQGFSVTGV--DPSPIAIERALL-----------NVRFDQFDEQEAAVPAG 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 220 ILDVILLVFVLSSIHPdrMQGVVNRLSKLLKPGGMLLFRDYGRYDKTQLRFKKGHCLSENfyvrgdGTRAYFFTKGEVHS 299
Cdd:pfam13489  80 KFDVIVAREVLEHVPD--PPALLRQIAALLKPGGLLLLSTPLASDEADRLLLEWPYLRPR------NGHISLFSARSLKR 151


                  ..
gi 1007374374 300 MF 301
Cdd:pfam13489 152 LL 153
Methyltransf_31 pfam13847
Methyltransferase domain; This family appears to have methyltransferase activity.
 149-301 2.77e-04

Methyltransferase domain; This family appears to have methyltransferase activity.


Pssm-ID: 463998 [Multi-domain]  Cd Length: 150  Bit Score: 40.86  E-value: 2.77e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 149 RILEVGCGAGNSVFpILNTLENsPESFLYCCDFASGAVELVKSHSSYRATQCFAFVH-DVCDdgLPYPFPDGILDVILLV 227
Cdd:pfam13847   6 RVLDLGCGTGHLSF-ELAEELG-PNAEVVGIDISEEAIEKARENAQKLGFDNVEFEQgDIEE--LPELLEDDKFDVVISN 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1007374374 228 FVLSSIhPDrMQGVVNRLSKLLKPGGMLLFRDYGRydktqLRFKKGHCLSENFYVRGDGTRAYffTKGEVHSMF 301
Cdd:pfam13847  82 CVLNHI-PD-PDKVLQEILRVLKPGGRLIISDPDS-----LAELPAHVKEDSTYYAGCVGGAI--LKKKLYELL 146
PLN02232 PLN02232
ubiquinone biosynthesis methyltransferase
 180-273 9.65e-04

ubiquinone biosynthesis methyltransferase


Pssm-ID: 165876  Cd Length: 160  Bit Score: 39.29  E-value: 9.65e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007374374 180 DFASGAVELVKSHSSYRATQCFAFVHDVCDDGLPYPFPDGILDVILLVFVLSSIhPDRMQGvVNRLSKLLKPGGMLLFRD 259
Cdd:PLN02232    4 DFSSEQLAVAATRQSLKARSCYKCIEWIEGDAIDLPFDDCEFDAVTMGYGLRNV-VDRLRA-MKEMYRVLKPGSRVSILD 81

                          90
                  ....*....|....
gi 1007374374 260 YGRYDKTQLRFKKG 273
Cdd:PLN02232   82 FNKSNQSVTTFMQG 95
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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