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Conserved domains on  [gi|1002255887|ref|XP_015632227|]
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protein NRT1/ PTR FAMILY 8.4 isoform X2 [Oryza sativa Japonica Group]

Protein Classification

MFS transporter( domain architecture ID 999995)

major facilitator superfamily (MFS) transporter facilitates the transport across cytoplasmic or internal membranes of one or more from a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides

CATH:  1.20.1250.20
Gene Ontology:  GO:0022857|GO:0055085
PubMed:  26758938|26098515
SCOP:  3000310
TCDB:  2.A.1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MFS super family cl28910
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 45-224 9.32e-67

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


The actual alignment was detected with superfamily member cd17418:

Pssm-ID: 475125 [Multi-domain]  Cd Length: 447  Bit Score: 214.64  E-value: 9.32e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  45 WKGSSLAIVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFI 124
Cdd:cd17418     1 WKACPFILGNECCERLAYYGIATNLITYLTKELHQGNVSAANNVTNWSGTCYITPLIGAFIADAYWGRYWTIASFSAIYF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 125 IGLMMLLLSAAVPLISTGPHSWIIWTDPVSSQNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKKSSFFNWT 204
Cdd:cd17418    81 IGMVLLTLSASVPGLKPASCIGSLCPPATSVQYAVFFLGLYLIALGTGGIKPCVSSFGADQFDDTDPKEKVRKSSFFNWF 160

                         170       180
                  ....*....|....*....|
gi 1002255887 205 YFVANAGSLISGTVIVWVQD 224
Cdd:cd17418   161 YFSINIGALISSSVLVWIQE 180
 
Name Accession Description Interval E-value
MFS_NPF8 cd17418
NRT1/PTR family (NPF), subfamily 8 of the Major Facilitator Superfamily of transporters; The ...
 45-224 9.32e-67

NRT1/PTR family (NPF), subfamily 8 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF8 contains the Arabidopsis dipeptide transporters AtNPF8.1 (PTR1), AtNPF8.2 (PTR5), and AtNPF8.3 (PTR2), as well as tonoplast-localized transporters AtNPF8.4 (PTR4) and AtNPF8.5 (PTR6). Oryza sativa NRT1 (now called OsNPF8.9) is a low-affinity nitrate transporter. NPF8 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340976 [Multi-domain]  Cd Length: 447  Bit Score: 214.64  E-value: 9.32e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  45 WKGSSLAIVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFI 124
Cdd:cd17418     1 WKACPFILGNECCERLAYYGIATNLITYLTKELHQGNVSAANNVTNWSGTCYITPLIGAFIADAYWGRYWTIASFSAIYF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 125 IGLMMLLLSAAVPLISTGPHSWIIWTDPVSSQNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKKSSFFNWT 204
Cdd:cd17418    81 IGMVLLTLSASVPGLKPASCIGSLCPPATSVQYAVFFLGLYLIALGTGGIKPCVSSFGADQFDDTDPKEKVRKSSFFNWF 160

                         170       180
                  ....*....|....*....|
gi 1002255887 205 YFVANAGSLISGTVIVWVQD 224
Cdd:cd17418   161 YFSINIGALISSSVLVWIQE 180
2A1704 TIGR00926
Peptide:H+ symporter (also transports b-lactam antibiotics, the antitumor agent, bestatin, and ...
 54-215 1.62e-19

Peptide:H+ symporter (also transports b-lactam antibiotics, the antitumor agent, bestatin, and various protease inhibitors); [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 273343 [Multi-domain]  Cd Length: 654  Bit Score: 88.28  E-value: 1.62e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  54 FSFCSYLAFtsivknLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLLLS 133
Cdd:TIGR00926   1 FSYYGMRTI------LVLYFLNFLGFSESTSTVLFHTFTYLCYLTPLIGAIIADGWLGKFKTILYLSIVYVVGHALLSFG 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 134 aAVPlISTGPhswiiWTDPVSsqniifFVGLYMVALGYGAQCPCISSFGADQFDDTDENERtkkSSFFNWTYFVANAGSL 213
Cdd:TIGR00926  75 -AIP-SSGHP-----LHDLLD------LLGLALIALGTGGIKPCVSAFGGDQFEERQLSLR---SRFFSFFYFAINAGSL 138


                  ..
gi 1002255887 214 IS 215
Cdd:TIGR00926 139 IS 140
PTR2 pfam00854
POT family; The POT (proton-dependent oligopeptide transport) family all appear to be proton ...
 113-228 3.38e-19

POT family; The POT (proton-dependent oligopeptide transport) family all appear to be proton dependent transporters.


Pssm-ID: 425909 [Multi-domain]  Cd Length: 392  Bit Score: 86.60  E-value: 3.38e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 113 YCTILIFCTIFIIGLMMLLLSAaVPLISTGPHSWIiwtdpvssqniiFFVGLYMVALGYGAQCPCISSFGADQFDDTDEN 192
Cdd:pfam00854   1 FRTIFLGSIIYAIGHILLALSA-IPSLRPPVHVAL------------FFIGLALIALGTGGIKPNVSSFGGDQFEETDPR 67

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1002255887 193 ERTKkssFFNWTYFVANAGSLISGTVIVWVQDHKDF 228
Cdd:pfam00854  68 RRSR---FFSIFYFSINAGSLISTIITPYLQQNYGY 100
PTR2 COG3104
Dipeptide/tripeptide permease [Amino acid transport and metabolism];
69-229 2.42e-15

Dipeptide/tripeptide permease [Amino acid transport and metabolism];


Pssm-ID: 442338 [Multi-domain]  Cd Length: 479  Bit Score: 75.62  E-value: 2.42e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  69 LVSYLTKVLHE-----TNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLLLSAAVPListgp 143
Cdd:COG3104    37 LVLYLTASLADgglglSEADAAAIYGAYTALVYLTPLLGGWLADRLLGQRRAILLGGILMALGHFLLALPGGVTL----- 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 144 hswiiwtdpvssqniiFFVGLYMVALGYGAQCPCISSFGADQFDDTDenerTKKSSFFNWTYFVANAGSLISGTVIVWVQ 223
Cdd:COG3104   112 ----------------FYLGLALIAVGNGLFKPNISSLVGQLYDPGD----PRRDAGFTIFYMGINIGAFLAPLLCGYLG 171


                  ....*.
gi 1002255887 224 DHKDFR 229
Cdd:COG3104   172 EKYGWH 177
PRK15462 PRK15462
dipeptide permease;
38-224 2.50e-04

dipeptide permease;


Pssm-ID: 237971 [Multi-domain]  Cd Length: 493  Bit Score: 42.24  E-value: 2.50e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  38 LRKHTGNWKGSSLAIVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTIL 117
Cdd:PRK15462    1 MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYDDNHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVM 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 118 IFCTIFIIGLMMLLLSAAVPlistgphswiiwtdpvssqnIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDenerTKK 197
Cdd:PRK15462   81 LGALLMAIGHVVLGASEIHP--------------------SFLYLSLAIIVCGYGLFKSNVSCLLGELYEPTD----PRR 136

                         170       180
                  ....*....|....*....|....*..
gi 1002255887 198 SSFFNWTYFVANAGSLISGTVIVWVQD 224
Cdd:PRK15462  137 DGGFSLMYAAGNVGSIIAPIACGYAQE 163
 
Name Accession Description Interval E-value
MFS_NPF8 cd17418
NRT1/PTR family (NPF), subfamily 8 of the Major Facilitator Superfamily of transporters; The ...
 45-224 9.32e-67

NRT1/PTR family (NPF), subfamily 8 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF8 contains the Arabidopsis dipeptide transporters AtNPF8.1 (PTR1), AtNPF8.2 (PTR5), and AtNPF8.3 (PTR2), as well as tonoplast-localized transporters AtNPF8.4 (PTR4) and AtNPF8.5 (PTR6). Oryza sativa NRT1 (now called OsNPF8.9) is a low-affinity nitrate transporter. NPF8 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340976 [Multi-domain]  Cd Length: 447  Bit Score: 214.64  E-value: 9.32e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  45 WKGSSLAIVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFI 124
Cdd:cd17418     1 WKACPFILGNECCERLAYYGIATNLITYLTKELHQGNVSAANNVTNWSGTCYITPLIGAFIADAYWGRYWTIASFSAIYF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 125 IGLMMLLLSAAVPLISTGPHSWIIWTDPVSSQNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKKSSFFNWT 204
Cdd:cd17418    81 IGMVLLTLSASVPGLKPASCIGSLCPPATSVQYAVFFLGLYLIALGTGGIKPCVSSFGADQFDDTDPKEKVRKSSFFNWF 160

                         170       180
                  ....*....|....*....|
gi 1002255887 205 YFVANAGSLISGTVIVWVQD 224
Cdd:cd17418   161 YFSINIGALISSSVLVWIQE 180
MFS_NPF5 cd17417
NRT1/PTR family (NPF), subfamily 5 of the Major Facilitator Superfamily of transporters; The ...
 45-225 8.63e-65

NRT1/PTR family (NPF), subfamily 5 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF5 includes Arabidopsis thaliana PTR3 (AtPTR3, now named AtNPF5.2), which is a wound-induced peptide transporter that is necessary for defense against virulent bacterial pathogens. NPF5 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340975 [Multi-domain]  Cd Length: 452  Bit Score: 209.82  E-value: 8.63e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  45 WKGSSLAIVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFI 124
Cdd:cd17417     1 WKACSFIIGIEVAERFAYYGIASNLVTYLTTPLHQSTARAAKNVNNWSGTATLLPLLGAFVADAYLGRYRTILISSLIYL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 125 IGLMMLLLSAAVPLISTgPHSWIIWTDPVSSQNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKKSSFFNWT 204
Cdd:cd17417    81 LGLVLLTLSASLPSLKP-PECNGTCSKASTLQVAFFFFSLYLVAIGQGGHKPCVQAFGADQFDEDDPKEKKQKSSFFNWW 159

                         170       180
                  ....*....|....*....|.
gi 1002255887 205 YFVANAGSLISGTVIVWVQDH 225
Cdd:cd17417   160 YFSICAGSLTAVTVLVYIQDN 180
MFS_NPF cd17351
Plant NRT1/PTR family (NPF) of the Major Facilitator Superfamily of transporters; The plant ...
 49-225 2.31e-64

Plant NRT1/PTR family (NPF) of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340909 [Multi-domain]  Cd Length: 445  Bit Score: 208.63  E-value: 2.31e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  49 SLAIVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLM 128
Cdd:cd17351     2 PFILGNEFAERLAFYGIAVNLVTYLTKVMHLSNAKAANTVTNFSGTSYLTPLLGAFLADAYLGRYWTIAIFSVIYLVGLI 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 129 MLLLSAAVPLIStgPHSWIIWTDPV---SSQNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKKSSFFNWTY 205
Cdd:cd17351    82 LLTLSASVPSLR--PPPCNPDSPCEpasSTQLTVLYVGLYLIALGTGGIKPCVSAFGADQFDETDPKEKKQKPSFFNWFY 159

                         170       180
                  ....*....|....*....|
gi 1002255887 206 FVANAGSLISGTVIVWVQDH 225
Cdd:cd17351   160 FSINLGSLLAVTLVVYIQDN 179
MFS_NPF7 cd17419
NRT1/PTR family (NPF), subfamily 7 of the Major Facilitator Superfamily of transporters; The ...
 45-224 1.66e-55

NRT1/PTR family (NPF), subfamily 7 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF7 includes the nitrate transporters AtNPF7.2 and AtNPF7.3, as well as the dipeptide transporter OsNPF7.3. AtNPF7.3 is a bidirectional transporter involved in nitrate influx and efflux. NPF7 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340977 [Multi-domain]  Cd Length: 447  Bit Score: 185.66  E-value: 1.66e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  45 WKGSSLAIVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFI 124
Cdd:cd17419     1 WKSAIFILVNQGLATLAYFGVGVNLVLYLTRVMHQSNASAANNVSNWTGTVYITPLIGAFLSDSYWGRYWTSTLFQAIFV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 125 IGLMMLLLSAAVPLI-STGPHSWIIWTDPVSS-QNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKKSSFFN 202
Cdd:cd17419    81 IGLLLLTLSSALPSLrPPQCGDGDLKCPPASAaQTTFLYFSLYLIALGYGGYQPCIATLGADQFDDEDPIEKASKSAFFS 160

                         170       180
                  ....*....|....*....|..
gi 1002255887 203 WTYFVANAGSLISGTVIVWVQD 224
Cdd:cd17419   161 YFYQALNLGSLFSNTILVYFED 182
MFS_NPF4 cd17414
NRT1/PTR family (NPF), subfamily 4 of the Major Facilitator Superfamily of transporters; The ...
 60-226 8.65e-40

NRT1/PTR family (NPF), subfamily 4 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. Members of the NPF4 subfamily have been shown to transport ABA. NPF4 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340972 [Multi-domain]  Cd Length: 456  Bit Score: 144.09  E-value: 8.65e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  60 LAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLLLSAAVPli 139
Cdd:cd17414    16 MAYLANANNLVTYLINNMHYSLAKSANIVTNFVGTAFLLALLGGFLSDSFLSSFKTIIIFGSIELLGLILLTVQAHIP-- 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 140 STGPHSWIIWTD-----PVSSQNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKKSSFFNWTYFVANAGSLI 214
Cdd:cd17414    94 SLQPPSCNSLGDltckePSGSQAAILFVGLYLVALGVGCIKPNLPSHGADQFDENDPKGRRQLSSFFNWYYFSFSLGALI 173

                         170
                  ....*....|..
gi 1002255887 215 SGTVIVWVQDHK 226
Cdd:cd17414   174 AVTLVVWVEENK 185
MFS_NPF3 cd17415
NRT1/PTR family (NPF), subfamily 3 of the Major Facilitator Superfamily of transporters; The ...
 45-225 9.90e-40

NRT1/PTR family (NPF), subfamily 3 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF3 is the smallest NPF subfamily and it includes Cucumis sativus nitrite transporter (CsNitr1), now named CsNPF3.2. It functions as a chloroplast nitrite uptake transporter to remove toxic nitrite from the cytosol. NPF3 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340973 [Multi-domain]  Cd Length: 448  Bit Score: 143.74  E-value: 9.90e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  45 WKGSSLAIVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFI 124
Cdd:cd17415     1 WKTMPFIFGNEICERLASVGFHTNLITYLTLQLHMPLVKAANILTNFNGTASLTPLLGAFIADSFAGRFWTITIGSIIYQ 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 125 IGLMMLLLSAAVPLISTGPHSW--IIWTDPVSSQNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKKSSFFN 202
Cdd:cd17415    81 LGMVLLTISAILPSLRPPPCNGheVCACQASSAQLSILYLSLLLTALGSGGIRPCVVAFGADQFDESDPKQAKQTWNFFN 160

                         170       180
                  ....*....|....*....|...
gi 1002255887 203 WTYFVANAGSLISGTVIVWVQDH 225
Cdd:cd17415   161 WYYFCMGIAILLAVTVIVYIQDN 183
MFS_NPF6 cd17413
NRT1/PTR family (NPF), subfamily 6 of the Major Facilitator Superfamily of transporters; The ...
 45-224 3.81e-39

NRT1/PTR family (NPF), subfamily 6 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter 1/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF6 includes the first identified member of the NRT1/PTR family: Arabidopsis thaliana NRT1.1, now called AtNPF6.3. It is a dual affinity nitrate influx transporter and a nitrate sensor. It also transports auxin and has nitrate efflux activity. NPF6 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340971 [Multi-domain]  Cd Length: 457  Bit Score: 142.59  E-value: 3.81e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  45 WKGSSLAIVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFI 124
Cdd:cd17413     1 WKSAALILGIEMCERLTTLGIGVNLVTYLTGTMHLPSATSANIVTNFLGTSFLLCLLGGFIADTFLGRYLTIAIFASIQA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 125 IGLMMLLLSAAVPLISTGP--HSWIIWTDPVSS-QNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKKSSFF 201
Cdd:cd17413    81 LGVLVLTLSTTLPSLRPPPcnHTTSHHCEPASGiQLTVLYLALYLIALGTGGIKSSVSGFGSDQFDENDPKERKLMSYFF 160

                         170       180
                  ....*....|....*....|...
gi 1002255887 202 NWTYFVANAGSLISGTVIVWVQD 224
Cdd:cd17413   161 NRFFFCISVGSLLAVTVLVYIQD 183
MFS_NPF1_2 cd17416
NRT1/PTR family (NPF), subfamily 1 and 2 of the Major Facilitator Superfamily of transporters; ...
 60-225 6.82e-37

NRT1/PTR family (NPF), subfamily 1 and 2 of the Major Facilitator Superfamily of transporters; The plant Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) is related to the POT (proton-coupled oligopeptide transporter), Peptide transporter (PepT/PTR), or Solute Carrier 15 (SLC15) family in animals. In contrast to related animal and bacterial counterparts, the plant proteins transport a wide variety of substrates including nitrate, peptides, amino acids, dicarboxylates, glucosinolates, as well as the plant hormones indole-3-acetic acid (IAA) and abscisic acid (ABA). A recent study identified eight subfamilies within this family, named NPF1-NPF8. NPF1 includes Medicago truncatula LATD/NIP, now named MtNPF1.7, which is a high-affinity nitrate transporter and is involved in nodulation and root architecture. NPF2 members are well-established nitrate and glucosinolate transporters, including Arabidopsis nitrate influx and efflux transporters with varied tissue and developmental specificity. Examples are AtNPF2.7, which is expressed in the cortex of mature roots, and AtNPF2.9, which is expressed in root companion cells where it is involved in phloem loading. NPF1/2 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340974 [Multi-domain]  Cd Length: 444  Bit Score: 136.24  E-value: 6.82e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  60 LAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLLLSAAVPLI 139
Cdd:cd17416    13 LASYGLLANLIVYLIEEYNMKSIAAANIINIWSGTTNFLPVVGAFLSDSYFGRFPTISVGSFASLLGMVLLTLTAAIPSL 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 140 ---STGPHSwIIWTDPVSSQNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKKSSFFNWTYFVANAGSLISG 216
Cdd:cd17416    93 rppPCETAS-SLCQSPSKLQLAVLYSALALLSIGAGGIRPCSLAFGADQFDKKTESGKRGLESFFNWYYFTFTVAQIISL 171


                  ....*....
gi 1002255887 217 TVIVWVQDH 225
Cdd:cd17416   172 TAIVYIQDN 180
MFS_SLC15A1_2_like cd17347
Solute carrier family 15 members 1 and 2, and similar Major Facilitator Superfamily ...
 52-228 6.18e-30

Solute carrier family 15 members 1 and 2, and similar Major Facilitator Superfamily transporters; Solute carrier family 15 member 1 (SLC15A1) and SLC15A2 are members of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. They mediate the proton-coupled active transport of a broad range of dipeptides and tripeptides, including zwitterionic, anionic and cationic peptides, as well as a variety of peptide-like drugs such as cefadroxil, enalapril, and valacyclovir. SLC15A1, or peptide transporter 1 (PepT1), is primarily expressed in the brush border membranes of enterocytes of the small intestine and is also known as the intestinal isoform. SLC15A2, or peptide transporter 2 (PepT2), is abundantly expressed in the apical membrane of kidney proximal tubules and is also referred to as the renal isoform. Both proteins transport di/tripeptides, but not tetrapeptides or free amino acids, using the energy generated by an inwardly directed transmembrane proton gradient. The SLC15A1/SLC15A2-like group belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340905 [Multi-domain]  Cd Length: 427  Bit Score: 116.95  E-value: 6.18e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  52 IVFSFC---SYLAFTSIvknLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLM 128
Cdd:cd17347     8 VGNEFCerfSYYGMRAI---LVLYLTDFLGFSEAEATAIYHSFVMLCYFFPLLGAIIADSYLGKYRTILYLSLVYCLGHI 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 129 MLLLSAAVPLisTGPHSWiiwtdpvssqniIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKkssFFNWTYFVA 208
Cdd:cd17347    85 VLAVTAIPPL--GPPHVW------------GLLIGLLLIAIGTGGIKPCVSAFGGDQFGADQQHLLAR---FFSWFYFAI 147

                         170       180
                  ....*....|....*....|
gi 1002255887 209 NAGSLISGTVIVWVQDHKDF 228
Cdd:cd17347   148 NAGSLLSTFLTPILRADVQF 167
MFS_POT cd06175
Proton-coupled oligopeptide transporter (POT) family of the Major Facilitator Superfamily of ...
 57-228 2.37e-28

Proton-coupled oligopeptide transporter (POT) family of the Major Facilitator Superfamily of transporters; The Proton-coupled oligopeptide transporter (POT) family is present across all major kingdoms of life and is known by a variety of names. It is referred to as the Nitrate transporter/Peptide transporter (NRT1/PTR) family (NPF) in plants, and in addition to POT, it is also known as the Peptide transporter (PepT/PTR) or Solute Carrier 15 (SLC15) family in animals. Members of this family are proton-driven symporters involved in nitrogen acquisition in the form of di- and tripeptides. Plant members transport other nitrogenous ligands including nitrate, the plant hormone auxin, and glucosinolate compounds that are important for seed defense. POT proteins exhibit substrate multispecificity, with one transporter able to recognize as many as 8,400 types of di/tripeptides and certain peptide-like drugs. The POT family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340865 [Multi-domain]  Cd Length: 422  Bit Score: 112.53  E-value: 2.37e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  57 CSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLLLSAAV 136
Cdd:cd06175    10 CERFTFYGIGANLVPFLTGALGLGNAQAANVVLNFLGTSYVTPLFGGWIADTFLGRYRTILIGAAIYAVGVAILTVSTIP 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 137 PLISTGPHSWIIWTDPVSSQNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDEnerTKKSSFFNWTYFVANAGSLISG 216
Cdd:cd06175    90 PGLRPLRCNPSHCEQASGIQLTGFYAALYLIALGTGGVKANVSPLGADQFDERGP---SKTQSFFNWFYFCINVGSLLAM 166

                         170
                  ....*....|..
gi 1002255887 217 TVIVWVQDHKDF 228
Cdd:cd06175   167 LGTPYIQDNVGW 178
2A1704 TIGR00926
Peptide:H+ symporter (also transports b-lactam antibiotics, the antitumor agent, bestatin, and ...
 54-215 1.62e-19

Peptide:H+ symporter (also transports b-lactam antibiotics, the antitumor agent, bestatin, and various protease inhibitors); [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 273343 [Multi-domain]  Cd Length: 654  Bit Score: 88.28  E-value: 1.62e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  54 FSFCSYLAFtsivknLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLLLS 133
Cdd:TIGR00926   1 FSYYGMRTI------LVLYFLNFLGFSESTSTVLFHTFTYLCYLTPLIGAIIADGWLGKFKTILYLSIVYVVGHALLSFG 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 134 aAVPlISTGPhswiiWTDPVSsqniifFVGLYMVALGYGAQCPCISSFGADQFDDTDENERtkkSSFFNWTYFVANAGSL 213
Cdd:TIGR00926  75 -AIP-SSGHP-----LHDLLD------LLGLALIALGTGGIKPCVSAFGGDQFEERQLSLR---SRFFSFFYFAINAGSL 138


                  ..
gi 1002255887 214 IS 215
Cdd:TIGR00926 139 IS 140
MFS_SLC15A1 cd17412
Solute Carrier family 15 member 1 of the Major Facilitator Superfamily of transporters; Solute ...
 52-219 2.16e-19

Solute Carrier family 15 member 1 of the Major Facilitator Superfamily of transporters; Solute carrier family 15 member 1 (SLC15A1), also called peptide transporter 1 (PepT1), is a member of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. SLC15A1, as well as SLC15A2, mediate the proton-coupled active transport of a broad range of dipeptides and tripeptides, including zwitterionic, anionic and cationic peptides, as well as a variety of peptide-like drugs such as cefadroxil, enalapril, and valacyclovir. SLC15A1 is primarily expressed in the brush border membranes of enterocytes of the small intestine and is also known as the intestinal isoform. It is a high-capacity/low-affinity transporter that drives the transport of di-and tripeptides for metabolic purposes. It's expression is upregulated in the colon during chronic inflammation associated with inflammatory bowel disease. SLC15A1/PepT1 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340970 [Multi-domain]  Cd Length: 415  Bit Score: 87.21  E-value: 2.16e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  52 IVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLL 131
Cdd:cd17412     8 VVNEFCERFSYYGMRAVLVLYFKYFLRWDDDLSTAIYHTFVALCYLTPILGAIIADSWLGKFKTIVYLSIVYTIGQAVMA 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 132 LSAAVPLISTGPHSWiiwTDPVSSQNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERtkkSSFFNWTYFVANAG 211
Cdd:cd17412    88 VSAIHDLTDANRDGT---PDNLTLHVALSMIGLLLIALGTGGIKPCVAAFGGDQFEEHQEKQR---SRFFSIFYLSINAG 161


                  ....*...
gi 1002255887 212 SLISgTVI 219
Cdd:cd17412   162 SLLS-TII 168
PTR2 pfam00854
POT family; The POT (proton-dependent oligopeptide transport) family all appear to be proton ...
 113-228 3.38e-19

POT family; The POT (proton-dependent oligopeptide transport) family all appear to be proton dependent transporters.


Pssm-ID: 425909 [Multi-domain]  Cd Length: 392  Bit Score: 86.60  E-value: 3.38e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 113 YCTILIFCTIFIIGLMMLLLSAaVPLISTGPHSWIiwtdpvssqniiFFVGLYMVALGYGAQCPCISSFGADQFDDTDEN 192
Cdd:pfam00854   1 FRTIFLGSIIYAIGHILLALSA-IPSLRPPVHVAL------------FFIGLALIALGTGGIKPNVSSFGGDQFEETDPR 67

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1002255887 193 ERTKkssFFNWTYFVANAGSLISGTVIVWVQDHKDF 228
Cdd:pfam00854  68 RRSR---FFSIFYFSINAGSLISTIITPYLQQNYGY 100
MFS_SLC15A2 cd17411
Solute carrier family 15 member 2 of the Major Facilitator Superfamily of transporters; Solute ...
 52-215 1.25e-18

Solute carrier family 15 member 2 of the Major Facilitator Superfamily of transporters; Solute carrier family 15 member 2 (SLC15A2), also called peptide transporter 2 (PepT2), is a member of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. SLC15A2, as well as SLC15A1, mediate the proton-coupled active transport of a broad range of dipeptides and tripeptides, including zwitterionic, anionic and cationic peptides, as well as a variety of peptide-like drugs such as cefadroxil, enalapril, and valacyclovir. SLC15A2 is a high-affinity transporter and is abundantly expressed in the apical membrane of kidney proximal tubules and choroid plexus epithelial cells. It is the major transporter involved in the reclamation of peptide-bound amino acids and peptide-like drugs in the kidney, and is also called the renal isoform. In choroid plexus and the brain, it acts as an efflux transporter and plays a role in regulating peptide/neuropeptide homeostasis. SLC15A2/PepT2 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340969 [Multi-domain]  Cd Length: 403  Bit Score: 84.88  E-value: 1.25e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  52 IVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLL 131
Cdd:cd17411     8 VVNEFCERFSYYGMKAVLTLYFLSFLHWDENLSTAIYHAFSGLCYFTPVLGALIADSWLGKFKTIIYLSIVYVVGHVIKS 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 132 LSAAVPLISTGPHSwiiwtdpvssqnIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDENERTKkssFFNWTYFVANAG 211
Cdd:cd17411    88 VGAIPTVGDQQVHV------------VLSMIGLFLIALGTGGIKPCVAAFGGDQFEEEHARERSK---FFSIFYLSINAG 152


                  ....
gi 1002255887 212 SLIS 215
Cdd:cd17411   153 SLIS 156
PTR2 COG3104
Dipeptide/tripeptide permease [Amino acid transport and metabolism];
69-229 2.42e-15

Dipeptide/tripeptide permease [Amino acid transport and metabolism];


Pssm-ID: 442338 [Multi-domain]  Cd Length: 479  Bit Score: 75.62  E-value: 2.42e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  69 LVSYLTKVLHE-----TNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLLLSAAVPListgp 143
Cdd:COG3104    37 LVLYLTASLADgglglSEADAAAIYGAYTALVYLTPLLGGWLADRLLGQRRAILLGGILMALGHFLLALPGGVTL----- 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 144 hswiiwtdpvssqniiFFVGLYMVALGYGAQCPCISSFGADQFDDTDenerTKKSSFFNWTYFVANAGSLISGTVIVWVQ 223
Cdd:COG3104   112 ----------------FYLGLALIAVGNGLFKPNISSLVGQLYDPGD----PRRDAGFTIFYMGINIGAFLAPLLCGYLG 171


                  ....*.
gi 1002255887 224 DHKDFR 229
Cdd:COG3104   172 EKYGWH 177
MFS_SLC15A3_4 cd17348
Solute Carrier family 15 members 3 and 4 of the Major Facilitator Superfamily of transporters; ...
 61-228 4.15e-15

Solute Carrier family 15 members 3 and 4 of the Major Facilitator Superfamily of transporters; Solute carrier family 15 members 3 (SLC15A3) and 4 (SLC15A4) are members of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. They are peptide/histidine transporters (PHTs) that transport free histidine in addition to di/tripeptides. SLC15A4, also called peptide transporter 4 or peptide/histidine transporter 1 (PHT1), is expressed in the human brain, retina, placenta, and immune cells. It is required for Toll-like receptor 7 (TLR7)- and TLR9-mediated type I interferon production in plasmacytoid dendritic cells (pDCs) and is involved in the pathogenesis of lupus-like autoimmunity. SLC15A3, also called osteoclast transporter, peptide transporter 3, or peptide/histidine transporter 2 (PHT2), is expressed in immune tissues including the spleen and thymus. The SLC15A3/SLC15A4 group belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340906 [Multi-domain]  Cd Length: 435  Bit Score: 74.83  E-value: 4.15e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  61 AFTSIVKNLVSYLT-KVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGlMMLLLSAAVPLI 139
Cdd:cd17348    17 AFYGITSNLVLFLNgSPFNWEGTQASQALLLFMGATYLLSPFGGWLADAYLGRFWTILLSLLLYLLG-MLLFPATAAPAL 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 140 stgphswiiwtdpvssqniifFVGLYMVALGYGAQCPCISSFGADQFDDTDEnERTKKssFFNWTYFVANAGSLISGTVI 219
Cdd:cd17348    96 ---------------------YAGLLLVGLGVGSVKANITPFGADQVKDRGP-EATRR--FFNWFYWSINLGAILSLGGV 151


                  ....*....
gi 1002255887 220 VWVQDHKDF 228
Cdd:cd17348   152 AYIQQNVSF 160
MFS_DtpA_like cd17346
Dipeptide and tripeptide permease A (DtpA)-like subfamily of the Major Facilitator Superfamily ...
 58-230 2.27e-12

Dipeptide and tripeptide permease A (DtpA)-like subfamily of the Major Facilitator Superfamily of transporters; The DtpA-like subfamily includes four Escherichia coli proteins: dipeptide and tripeptide permeases A (DtpA, TppB or YdgR), B (DtpB or YhiP), C (DtpC or YjdL), and D (DtpD or YbgH). They are proton-dependent permeases that transport di- and tripeptides. DtpA and DtpB display a preference for di- and tripeptides composed of L-amino acids. DtpC shows higher specificity for dipeptides compared to tripeptides, and prefers dipeptides containing a C-terminal lysine residue. The DtpA-like subfamily belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340904 [Multi-domain]  Cd Length: 399  Bit Score: 66.44  E-value: 2.27e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  58 SYLAFTSIvknLVSYLT-KVLHETNVA-AARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLLLSaa 135
Cdd:cd17346    17 SYYGMRAI---LILYLTaQLGDGFSEAdAASIYGAYTALVYLTPIIGGWLADKVLGARRTVFIGGILMALGYLLLALS-- 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 136 vplistgphswiiwtdpvSSQNIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDEnertKKSSFFNWTYFVANAGSLIS 215
Cdd:cd17346    92 ------------------PFSVSMLYLGLALIIVGNGLFKPNISSLVGKLYDENDP----RRDSGFSIFYMGINLGAFLA 149

                         170
                  ....*....|....*
gi 1002255887 216 GTVIVWVQDHKDFRN 230
Cdd:cd17346   150 PLLCGTLGDNYGYHY 164
MFS_SLC15A5 cd17349
Solute Carrier family 15 member 5 of the Major Facilitator Superfamily of transporters; Solute ...
 57-223 1.21e-08

Solute Carrier family 15 member 5 of the Major Facilitator Superfamily of transporters; Solute carrier family 15 member 5 (SLC15A5) is a member of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. The specific function of SLC15A5 is unknown. SLC15A5 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340907 [Multi-domain]  Cd Length: 437  Bit Score: 55.53  E-value: 1.21e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  57 CSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLLLsAAV 136
Cdd:cd17349    13 CERFTFFGIVCNMILFCTIKLGYRNHQAAIVNLCFVGTSTLTPVFVGWLAEECMGRNKLVYICLFLHFLGTALLPV-VAF 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 137 PL--ISTGPHSwIIWTDPVSSQNIIFFVGLYMVALGYGA----QCPcISSFGadqfddTDENERTKKSSFFNWTYFVANA 210
Cdd:cd17349    92 PFedFYIDTHH-IIHTLAKREQTLLFYVALLTASLGTGGiraiVCP-LSAYG------LQDYGSRKLMSFFNWFYWLVNL 163

                         170
                  ....*....|...
gi 1002255887 211 GSLISGTVIVWVQ 223
Cdd:cd17349   164 NSAVVFVGISYIQ 176
MFS_PTR2 cd17350
Peptide transporter PTR2 of the Major Facilitator Superfamily of transporters; Fungal peptide ...
 96-213 2.63e-05

Peptide transporter PTR2 of the Major Facilitator Superfamily of transporters; Fungal peptide transporter or permease PTR2 is a member of the proton-coupled oligopeptide transporter (POT) family of integral membrane proteins that mediate the cellular uptake of di/tripeptides and peptide-like drugs. It is a 12-transmembrane domain (TMD) integral membrane protein that translocates di-/tripeptides. As with other POT family proteins, it displays characteristic substrate multispecificity. PTR2 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340908 [Multi-domain]  Cd Length: 438  Bit Score: 45.15  E-value: 2.63e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  96 YLAPLVGAFLADSYLGKYCTILIFCTIFIIGLMMLLLSAAVPLISTGphswiiwtdpvsSQNIIFFVGLYMVALGYGAQC 175
Cdd:cd17350    60 YVTPVIGAYVADQFLGRYNTIVCGAVIYFVGILILTCTSIPSVIDAG------------AGLGGFVVAIIIIGLGTGGIK 127

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1002255887 176 PCISSFGADQFDDTDENERTKK-------------SSFFNWTYFVANAGSL 213
Cdd:cd17350   128 SNVSPLIADQLPKIKPYVKTLKngervivdpvvtlSRIYMIFYWAINVGSL 178
PRK15462 PRK15462
dipeptide permease;
38-224 2.50e-04

dipeptide permease;


Pssm-ID: 237971 [Multi-domain]  Cd Length: 493  Bit Score: 42.24  E-value: 2.50e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  38 LRKHTGNWKGSSLAIVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADSYLGKYCTIL 117
Cdd:PRK15462    1 MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYDDNHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVM 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 118 IFCTIFIIGLMMLLLSAAVPlistgphswiiwtdpvssqnIIFFVGLYMVALGYGAQCPCISSFGADQFDDTDenerTKK 197
Cdd:PRK15462   81 LGALLMAIGHVVLGASEIHP--------------------SFLYLSLAIIVCGYGLFKSNVSCLLGELYEPTD----PRR 136

                         170       180
                  ....*....|....*....|....*..
gi 1002255887 198 SSFFNWTYFVANAGSLISGTVIVWVQD 224
Cdd:PRK15462  137 DGGFSLMYAAGNVGSIIAPIACGYAQE 163
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
50-229 1.09e-03

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 40.24  E-value: 1.09e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887  50 LAIVFSFCSYLAFTSIVKNLVSYLTKVLHETNVAAARDVATWSGTSYLAPLVGAFLADsYLGKYCTILIFCTIFIIGLMM 129
Cdd:COG2271   184 LLALAYFLVYFALYGFLTWLPTYLVEVRGLSLAQAGLLLSLPFLAGIVGSLLGGWLSD-RLGRRRKLVLAIGLLLAALAL 262

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1002255887 130 LLLSAAVPListgphswiiwtdpvssqnIIFFVGLYMVALGYGAQCPCISSFGADQFDdtdeneRTKKSSFFNWTYFVAN 209
Cdd:COG2271   263 LLLALLPSP-------------------ALAIALLFLAGFGLGGAFGLLWALAAELFP------KKARGTASGLVNTFGF 317

                         170       180
                  ....*....|....*....|
gi 1002255887 210 AGSLISGTVIVWVQDHKDFR 229
Cdd:COG2271   318 LGGALGPLLVGYLLDATGYQ 337
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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