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Conserved domains on  [gi|66824663|ref|XP_645686|]
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hypothetical protein DDB_G0271268 [Dictyostelium discoideum AX4]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11441181)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
3-186 5.15e-44

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


:

Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 143.60  E-value: 5.15e-44
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663   3 EIKIRNVNENDIKEITNIYKHYVLNEVCTFEEEVPSEEEMNKRRSIIVDKNFPYIVATTttttttiikDGAnditkeiVI 82
Cdd:COG1247   1 EMTIRPATPEDAPAIAAIYNEAIAEGTATFETEPPSEEEREAWFAAILAPGRPVLVAEE---------DGE-------VV 64

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663  83 GYAYASSFRPRAAYRFTVEDSIYIDINYQKLGVGSILLKELISQCKEKGFKEIIAVIAAggPNQVggsSIKLHKKFGFDN 162
Cdd:COG1247  65 GFASLGPFRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLA--DNEA---SIALYEKLGFEE 139

                       170       180
                ....*....|....*....|....
gi 66824663 163 QHTLKNVGYKFNQFLDTVSLQLSL 186
Cdd:COG1247 140 VGTLPEVGFKFGRWLDLVLMQKRL 163
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
3-186 5.15e-44

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 143.60  E-value: 5.15e-44
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663   3 EIKIRNVNENDIKEITNIYKHYVLNEVCTFEEEVPSEEEMNKRRSIIVDKNFPYIVATTttttttiikDGAnditkeiVI 82
Cdd:COG1247   1 EMTIRPATPEDAPAIAAIYNEAIAEGTATFETEPPSEEEREAWFAAILAPGRPVLVAEE---------DGE-------VV 64

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663  83 GYAYASSFRPRAAYRFTVEDSIYIDINYQKLGVGSILLKELISQCKEKGFKEIIAVIAAggPNQVggsSIKLHKKFGFDN 162
Cdd:COG1247  65 GFASLGPFRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLA--DNEA---SIALYEKLGFEE 139

                       170       180
                ....*....|....*....|....
gi 66824663 163 QHTLKNVGYKFNQFLDTVSLQLSL 186
Cdd:COG1247 140 VGTLPEVGFKFGRWLDLVLMQKRL 163
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 81-160 4.09e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 52.13  E-value: 4.09e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663    81 VIGYAYASSFRPRaaYRFTVEDSIYIDINYQKLGVGSILLKELISQCKEKGFKEIIAVIAAggPNQvggSSIKLHKKFGF 160
Cdd:pfam00583  44 LVGFASLSIIDDE--PPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAA--DNL---AAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 81-136 2.24e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.03  E-value: 2.24e-04
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 66824663  81 VIGYAyasSFRPRAAYRFTVE-DSIYIDINYQKLGVGSILLKELISQCKEKGFKEII 136
Cdd:cd04301  10 IVGFA---SLSPDGSGGDTAYiGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLR 63
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
3-186 5.15e-44

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 143.60  E-value: 5.15e-44
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663   3 EIKIRNVNENDIKEITNIYKHYVLNEVCTFEEEVPSEEEMNKRRSIIVDKNFPYIVATTttttttiikDGAnditkeiVI 82
Cdd:COG1247   1 EMTIRPATPEDAPAIAAIYNEAIAEGTATFETEPPSEEEREAWFAAILAPGRPVLVAEE---------DGE-------VV 64

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663  83 GYAYASSFRPRAAYRFTVEDSIYIDINYQKLGVGSILLKELISQCKEKGFKEIIAVIAAggPNQVggsSIKLHKKFGFDN 162
Cdd:COG1247  65 GFASLGPFRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLA--DNEA---SIALYEKLGFEE 139

                       170       180
                ....*....|....*....|....
gi 66824663 163 QHTLKNVGYKFNQFLDTVSLQLSL 186
Cdd:COG1247 140 VGTLPEVGFKFGRWLDLVLMQKRL 163
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 81-160 4.09e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 52.13  E-value: 4.09e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663    81 VIGYAYASSFRPRaaYRFTVEDSIYIDINYQKLGVGSILLKELISQCKEKGFKEIIAVIAAggPNQvggSSIKLHKKFGF 160
Cdd:pfam00583  44 LVGFASLSIIDDE--PPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAA--DNL---AAIALYEKLGF 116
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 77-182 4.22e-07

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 46.97  E-value: 4.22e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663  77 TKEIVIGYAYASSFRPRAAYrftVEDsIYIDINYQKLGVGSILLKELISQCKEKGFKEIIAVIAAGGPnqvggSSIKLHK 156
Cdd:COG0454  41 DKGEPIGFAGLRRLDDKVLE---LKR-LYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNP-----AAIRFYE 111

                        90       100
                ....*....|....*....|....*.
gi 66824663 157 KFGFDNQHTLKNvgYKFNQFLDTVSL 182
Cdd:COG0454 112 RLGFKEIERYVA--YVGGEFEKELSL 135
Acetyltransf_4 pfam13420
Acetyltransferase (GNAT) domain;
6-178 2.88e-06

Acetyltransferase (GNAT) domain;


Pssm-ID: 433192 [Multi-domain]  Cd Length: 153  Bit Score: 45.05  E-value: 2.88e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663     6 IRNVNENDIKEITNIYKHYVLNEVCTFEEEVPSEEEMNKR-RSIIVDKNFPYIVATtttttttiikdganditKEIVIGY 84
Cdd:pfam13420   1 IRALTQNDLKEIRRWYAEDRVNPAFTQEYAHSSIEEFETFlAAYLSPGEIVFGVAE-----------------SDRLIGY 63

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663    85 AYASSFRPRaaYRFTVEDSIYIDINYQKlGVGSILLKELIS-QCKEKGFKEIIAVIAAGGPNqvggsSIKLHKKFGFDNQ 163
Cdd:pfam13420  64 ATLRQFDYV--KTHKAELSFYVVKNNDE-GINRELINAIIQyARKNQNIENLEACIASNNIN-----AIVFLKAIGFEWL 135

                         170
                  ....*....|....*
gi 66824663   164 HTLKNVGYKFNQFLD 178
Cdd:pfam13420 136 GIERNAIKKNGRWID 150
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 4-160 2.92e-05

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 41.90  E-value: 2.92e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663   4 IKIRNVNENDIKEITNIYKHYVLNEvctfeeevpseeemNKRRsiivdknfpYIVATtttttttiiKDGAnditkeiVIG 83
Cdd:COG1246   1 MTIRPATPDDVPAILELIRPYALEE--------------EIGE---------FWVAE---------EDGE-------IVG 41

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 66824663  84 YAYASSFRPRAAY-RftvedSIYIDINYQKLGVGSILLKELISQCKEKGFKEIIAViaaggpnqVGGSSIKLHKKFGF 160
Cdd:COG1246  42 CAALHPLDEDLAElR-----SLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLL--------TTSAAIHFYEKLGF 106
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 85-160 3.84e-05

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 40.79  E-value: 3.84e-05
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 66824663  85 AYASSFRPRAAYRFTVEDsIYIDINYQKLGVGSILLKELISQCKEKGFKEIIAVIAAGGPnqvggSSIKLHKKFGF 160
Cdd:COG0456   1 GFALLGLVDGGDEAEIED-LAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNE-----AAIALYEKLGF 70
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
81-177 1.63e-04

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 40.07  E-value: 1.63e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663  81 VIGYAYASSFR-PRAAYRFTVEDsIYIDINYQKLGVGSILLKELISQCKEKGFKEIIAViaaGGPNQVGgssikLHKKFG 159
Cdd:COG3153  50 IVGHVALSPVDiDGEGPALLLGP-LAVDPEYRGQGIGRALMRAALEAARERGARAVVLL---GDPSLLP-----FYERFG 120

                        90
                ....*....|....*...
gi 66824663 160 FDNQHTLKNVGYKFNQFL 177
Cdd:COG3153 121 FRPAGELGLTLGPDEVFL 138
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 81-136 2.24e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.03  E-value: 2.24e-04
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 66824663  81 VIGYAyasSFRPRAAYRFTVE-DSIYIDINYQKLGVGSILLKELISQCKEKGFKEII 136
Cdd:cd04301  10 IVGFA---SLSPDGSGGDTAYiGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLR 63
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 81-160 2.25e-03

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 35.51  E-value: 2.25e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663    81 VIGYAyasSFRPRAAYRFTVEDSIYIDINYQKLGVGSILLKELISQCKEKGFKEIIAviaaggpnQVGGSSIKLHKKFGF 160
Cdd:pfam13508  14 IVGFA---ALLPLDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLEL--------ETTNRAAAFYEKLGF 82
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 81-180 9.08e-03

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 35.36  E-value: 9.08e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66824663  81 VIGYAyasSFRPRAAYRFTVEDSIYIDINYQKLGVGSILLKELISQCKEK-GFKEIIAVIAAGgpNQvggSSIKLHKKFG 159
Cdd:COG1670  73 LIGVV---GLYDIDRANRSAEIGYWLAPAYWGKGYATEALRALLDYAFEElGLHRVEAEVDPD--NT---ASIRVLEKLG 144

                        90       100
                ....*....|....*....|.
gi 66824663 160 FDNQHTLKNVGYKFNQFLDTV 180
Cdd:COG1670 145 FRLEGTLRDALVIDGRYRDHV 165
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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