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Conserved domains on  [gi|2462546738|ref|XP_054235197|]
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ubiquitin-conjugating enzyme E2 Q2 isoform X10 [Homo sapiens]

Protein Classification

RWD domain-containing protein( domain architecture ID 5491)

RWD domain-containing protein similar to Homo sapiens RWD domain-containing protein 3 (RWDD3), also known as RSUME, which is a RWD-containing protein that enhances SUMO conjugation by interacting with the SUMO conjugase Ubc9, increases Ubc9 thioester formation and therefore favours sumoylation of specific targets

CATH:  3.10.110.10
Gene Ontology:  GO:0005515
PubMed:  26276859|25918163

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
UBCc_UEV super family cl49610
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain/ubiquitin E2 variant (UEV) domain; ...
 148-184 2.93e-08

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain/ubiquitin E2 variant (UEV) domain; The family includes ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain and ubiquitin (Ub) E2 variant (UEV) domain. They belong to the ubiquitin-conjugating (UBC) superfamily that represents a structural domain with an alpha-beta(4)-alpha(3) core fold. E2 is part of the ubiquitin-mediated protein degradation pathway in which a thioester linkage forms between a conserved cysteine and the C-terminus of ubiquitin, and complexes with ubiquitin protein ligase enzymes, E3. This pathway regulates many fundamental cellular processes. There are also other E2s which form thioester linkages without the use of E3s. Several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins) contains the UEV domain, which lacks the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.


The actual alignment was detected with superfamily member cd23802:

Pssm-ID: 483950  Cd Length: 157  Bit Score: 50.71  E-value: 2.93e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 2462546738 148 LEKIRKTQRQDHL------NDNFPFDPPFVRVVLPVLSGGLVH 184
Cdd:cd23802    49 LKKLKKKHGYDYIelelrfPDLYPFYPPFVRVVRPRLKGGTGH 91
RWD super family cl02687
RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. ...
 9-89 1.80e-04

RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices.


The actual alignment was detected with superfamily member pfam05773:

Pssm-ID: 413438  Cd Length: 111  Bit Score: 39.61  E-value: 1.80e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462546738   9 ELKFLASIFDKNHERFRIVSWKLDELHCQFLVPQQGSPHSLPPPLTLHCNITESYPSSSPIWFVDS----EDPNLTSVLE 84
Cdd:pfam05773   6 ELEALESIYPDEFEVISDSPYESLEIEIKLSLDSDESDSSHLPPLVLKFTLPEDYPDEPPKISLSSpwnlSDEQVLSLLE 85


                  ....*
gi 2462546738  85 RLEDT 89
Cdd:pfam05773  86 ELEEL 90
 
Name Accession Description Interval E-value
UBCc_UBE2Q cd23802
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 Q1, ...
 148-184 2.93e-08

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 Q1, Q2, and related proteins; The E2Q subfamily includes mammalian ubiquitin-conjugating enzymes E2 Q1 (UBE2Q1/NICE5/UBE2Q), Q2 (UBE2Q2), and similar proteins. They are ubiquitin-conjugating (EC 2.3.2.23) enzymes that accept ubiquitin from the E1 complex and catalyze the covalent attachment to other proteins. UBE2Q1 may be involved in hormonal homeostasis in females. It is involved in regulation of B4GALT1 cell surface expression, B4GALT1-mediated cell adhesion to laminin and embryoid body formation. In vitro, UBE2Q2 catalyzes 'Lys-48'-linked polyubiquitination. This subfamily also includes ubiquitin-conjugating enzyme E2Q-like protein 1 (UBE2QL1), which is a probable E2 ubiquitin-protein ligase that may facilitate the monoubiquitination and degradation of MTOR and CCNE1 through interaction with FBXW7.


Pssm-ID: 467422  Cd Length: 157  Bit Score: 50.71  E-value: 2.93e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 2462546738 148 LEKIRKTQRQDHL------NDNFPFDPPFVRVVLPVLSGGLVH 184
Cdd:cd23802    49 LKKLKKKHGYDYIelelrfPDLYPFYPPFVRVVRPRLKGGTGH 91
RWD pfam05773
RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. ...
 9-89 1.80e-04

RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices.


Pssm-ID: 399058  Cd Length: 111  Bit Score: 39.61  E-value: 1.80e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462546738   9 ELKFLASIFDKNHERFRIVSWKLDELHCQFLVPQQGSPHSLPPPLTLHCNITESYPSSSPIWFVDS----EDPNLTSVLE 84
Cdd:pfam05773   6 ELEALESIYPDEFEVISDSPYESLEIEIKLSLDSDESDSSHLPPLVLKFTLPEDYPDEPPKISLSSpwnlSDEQVLSLLE 85


                  ....*
gi 2462546738  85 RLEDT 89
Cdd:pfam05773  86 ELEEL 90
 
Name Accession Description Interval E-value
UBCc_UBE2Q cd23802
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 Q1, ...
 148-184 2.93e-08

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 Q1, Q2, and related proteins; The E2Q subfamily includes mammalian ubiquitin-conjugating enzymes E2 Q1 (UBE2Q1/NICE5/UBE2Q), Q2 (UBE2Q2), and similar proteins. They are ubiquitin-conjugating (EC 2.3.2.23) enzymes that accept ubiquitin from the E1 complex and catalyze the covalent attachment to other proteins. UBE2Q1 may be involved in hormonal homeostasis in females. It is involved in regulation of B4GALT1 cell surface expression, B4GALT1-mediated cell adhesion to laminin and embryoid body formation. In vitro, UBE2Q2 catalyzes 'Lys-48'-linked polyubiquitination. This subfamily also includes ubiquitin-conjugating enzyme E2Q-like protein 1 (UBE2QL1), which is a probable E2 ubiquitin-protein ligase that may facilitate the monoubiquitination and degradation of MTOR and CCNE1 through interaction with FBXW7.


Pssm-ID: 467422  Cd Length: 157  Bit Score: 50.71  E-value: 2.93e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|...
gi 2462546738 148 LEKIRKTQRQDHL------NDNFPFDPPFVRVVLPVLSGGLVH 184
Cdd:cd23802    49 LKKLKKKHGYDYIelelrfPDLYPFYPPFVRVVRPRLKGGTGH 91
RWD pfam05773
RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. ...
 9-89 1.80e-04

RWD domain; This domain was identified in WD40 repeat proteins and Ring finger domain proteins. The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices.


Pssm-ID: 399058  Cd Length: 111  Bit Score: 39.61  E-value: 1.80e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462546738   9 ELKFLASIFDKNHERFRIVSWKLDELHCQFLVPQQGSPHSLPPPLTLHCNITESYPSSSPIWFVDS----EDPNLTSVLE 84
Cdd:pfam05773   6 ELEALESIYPDEFEVISDSPYESLEIEIKLSLDSDESDSSHLPPLVLKFTLPEDYPDEPPKISLSSpwnlSDEQVLSLLE 85


                  ....*
gi 2462546738  85 RLEDT 89
Cdd:pfam05773  86 ELEEL 90
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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