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Conserved domains on  [gi|2287722447|ref|XP_050008234|]
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myosin regulatory light chain 12B-like [Alexandromys fortis]

Protein Classification

EF-hand domain-containing protein( domain architecture ID 1000080)

EF-hand (EFh) domain-containing protein may be involved in binding intracellular calcium and in calcium signal transduction

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PTZ00184 super family cl33172
calmodulin; Provisional
 1-124 5.37e-26

calmodulin; Provisional


The actual alignment was detected with superfamily member PTZ00184:

Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 95.21  E-value: 5.37e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2287722447   1 MIDQNRDGFIDKDDLHDMLASLGKNPTDAYLDAMMNE----AAGPINFTMFLAMFGKKLNGTDPEDVIRNAFACFDEEAT 76
Cdd:PTZ00184   19 LFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEvdadGNGTIDFPEFLTLMARKMKDTDSEEEIKEAFKVFDRDGN 98

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2287722447  77 GTIQENYLRELLTMVGDRLADEEVDELYREAPIDKKGSFNYIQFTRIL 124
Cdd:PTZ00184   99 GFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFVKMM 146
 
Name Accession Description Interval E-value
PTZ00184 PTZ00184
calmodulin; Provisional
 1-124 5.37e-26

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 95.21  E-value: 5.37e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2287722447   1 MIDQNRDGFIDKDDLHDMLASLGKNPTDAYLDAMMNE----AAGPINFTMFLAMFGKKLNGTDPEDVIRNAFACFDEEAT 76
Cdd:PTZ00184   19 LFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEvdadGNGTIDFPEFLTLMARKMKDTDSEEEIKEAFKVFDRDGN 98

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2287722447  77 GTIQENYLRELLTMVGDRLADEEVDELYREAPIDKKGSFNYIQFTRIL 124
Cdd:PTZ00184   99 GFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFVKMM 146
EFh_PEF_ALG-2_like cd16185
EF-hand, calcium binding motif, found in homologs of mammalian apoptosis-linked gene 2 protein ...
 1-123 3.85e-05

EF-hand, calcium binding motif, found in homologs of mammalian apoptosis-linked gene 2 protein (ALG-2); The family includes some homologs of mammalian apoptosis-linked gene 2 protein (ALG-2) mainly found in lower eukaryotes, such as a parasitic protist Leishmarua major and a cellular slime mold Dictyostelium discoideum. These homologs contains five EF-hand motifs. Due to the presence of unfavorable residues at the Ca2+-coordinating positions, their non-canonical EF4 and EF5 hands may not bind Ca2+. Two Dictyostelium PEF proteins are the prototypes of this family. They may bind to cytoskeletal proteins and/or signal-transducing proteins localized to detergent-resistant membranes named lipid rafts, and occur as monomers or weak homo- or heterodimers like ALG-2. They can serve as a mediator for Ca2+ signaling-related Dictyostehum programmed cell death (PCD).


Pssm-ID: 320060 [Multi-domain]  Cd Length: 163  Bit Score: 41.05  E-value: 3.85e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2287722447   1 MIDQNRDGFIDKDDLHDMLASLGKNPTDAYLDAMMN----EAAGPINFTMFLAMFGKKLNgtdpedvIRNAFACFDEEAT 76
Cdd:cd16185     8 AVDRDRSGSIDVNELQKALAGGGLLFSLATAEKLIRmfdrDGNGTIDFEEFAALHQFLSN-------MQNGFEQRDTSRS 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 2287722447  77 GTIQENYLRELLTMVGDRLADEEVDELYREAPIDKKGSFNYIQFTRI 123
Cdd:cd16185    81 GRLDANEVHEALAASGFQLDPPAFQALFRKFDPDRGGSLGFDDYIEL 127
EF-hand_11 pfam08976
EF-hand domain; This domain is found predominantly in DJ binding proteins.
 64-120 1.85e-04

EF-hand domain; This domain is found predominantly in DJ binding proteins.


Pssm-ID: 401068  Cd Length: 105  Bit Score: 38.16  E-value: 1.85e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 2287722447  64 IRNAFACFDEEATGTIQENYLRELLTMVGDRLADEEVDELYREAPIDKKGSFNYIQF 120
Cdd:pfam08976  22 ITQEFENFDTLKSNTISRDEFRAICNRHIQILTDEQFDRLWNELPVNAKGRLKYPDF 78
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
1-114 7.32e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 34.38  E-value: 7.32e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2287722447   1 MIDQNRDGFIDKDDLHDMLASLgknpTDAYLDAMMNEAAGPINFTMFLAMFGKKLNGTDpEDVIRNAFACFDEEATGTIQ 80
Cdd:COG5126    13 LLDADGDGVLERDDFEALFRRL----WATLFSEADTDGDGRISREEFVAGMESLFEATV-EPFARAAFDLLDTDGDGKIS 87

                          90       100       110
                  ....*....|....*....|....*....|....
gi 2287722447  81 ENYLRELLTMVGdrLADEEVDELYREAPIDKKGS 114
Cdd:COG5126    88 ADEFRRLLTALG--VSEEEADELFARLDTDGDGK 119
 
Name Accession Description Interval E-value
PTZ00184 PTZ00184
calmodulin; Provisional
 1-124 5.37e-26

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 95.21  E-value: 5.37e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2287722447   1 MIDQNRDGFIDKDDLHDMLASLGKNPTDAYLDAMMNE----AAGPINFTMFLAMFGKKLNGTDPEDVIRNAFACFDEEAT 76
Cdd:PTZ00184   19 LFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEvdadGNGTIDFPEFLTLMARKMKDTDSEEEIKEAFKVFDRDGN 98

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2287722447  77 GTIQENYLRELLTMVGDRLADEEVDELYREAPIDKKGSFNYIQFTRIL 124
Cdd:PTZ00184   99 GFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFVKMM 146
PTZ00183 PTZ00183
centrin; Provisional
 1-125 1.01e-12

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 61.24  E-value: 1.01e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2287722447   1 MIDQNRDGFIDKDDLHDMLASLGKNPTDAYLDAMM----NEAAGPINFTMFLAMFGKKLNGTDPEDVIRNAFACFDEEAT 76
Cdd:PTZ00183   25 LFDTDGSGTIDPKELKVAMRSLGFEPKKEEIKQMIadvdKDGSGKIDFEEFLDIMTKKLGERDPREEILKAFRLFDDDKT 104

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 2287722447  77 GTIQENYLRELLTMVGDRLADEEVDELYREAPIDKKGSFNYIQFTRILK 125
Cdd:PTZ00183  105 GKISLKNLKRVAKELGETITDEELQEMIDEADRNGDGEISEEEFYRIMK 153
EFh_PEF_ALG-2_like cd16185
EF-hand, calcium binding motif, found in homologs of mammalian apoptosis-linked gene 2 protein ...
 1-123 3.85e-05

EF-hand, calcium binding motif, found in homologs of mammalian apoptosis-linked gene 2 protein (ALG-2); The family includes some homologs of mammalian apoptosis-linked gene 2 protein (ALG-2) mainly found in lower eukaryotes, such as a parasitic protist Leishmarua major and a cellular slime mold Dictyostelium discoideum. These homologs contains five EF-hand motifs. Due to the presence of unfavorable residues at the Ca2+-coordinating positions, their non-canonical EF4 and EF5 hands may not bind Ca2+. Two Dictyostelium PEF proteins are the prototypes of this family. They may bind to cytoskeletal proteins and/or signal-transducing proteins localized to detergent-resistant membranes named lipid rafts, and occur as monomers or weak homo- or heterodimers like ALG-2. They can serve as a mediator for Ca2+ signaling-related Dictyostehum programmed cell death (PCD).


Pssm-ID: 320060 [Multi-domain]  Cd Length: 163  Bit Score: 41.05  E-value: 3.85e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2287722447   1 MIDQNRDGFIDKDDLHDMLASLGKNPTDAYLDAMMN----EAAGPINFTMFLAMFGKKLNgtdpedvIRNAFACFDEEAT 76
Cdd:cd16185     8 AVDRDRSGSIDVNELQKALAGGGLLFSLATAEKLIRmfdrDGNGTIDFEEFAALHQFLSN-------MQNGFEQRDTSRS 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 2287722447  77 GTIQENYLRELLTMVGDRLADEEVDELYREAPIDKKGSFNYIQFTRI 123
Cdd:cd16185    81 GRLDANEVHEALAASGFQLDPPAFQALFRKFDPDRGGSLGFDDYIEL 127
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 1-51 6.83e-05

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 38.30  E-value: 6.83e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 2287722447   1 MIDQNRDGFIDKDDLHDMLASLGKNPTDAYLDAMMNEA----AGPINFTMFLAMF 51
Cdd:cd00051     8 LFDKDGDGTISADELKAALKSLGEGLSEEEIDEMIREVdkdgDGKIDFEEFLELM 62
EF-hand_11 pfam08976
EF-hand domain; This domain is found predominantly in DJ binding proteins.
 64-120 1.85e-04

EF-hand domain; This domain is found predominantly in DJ binding proteins.


Pssm-ID: 401068  Cd Length: 105  Bit Score: 38.16  E-value: 1.85e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 2287722447  64 IRNAFACFDEEATGTIQENYLRELLTMVGDRLADEEVDELYREAPIDKKGSFNYIQF 120
Cdd:pfam08976  22 ITQEFENFDTLKSNTISRDEFRAICNRHIQILTDEQFDRLWNELPVNAKGRLKYPDF 78
EFh_calglandulin_like cd16252
EF-hand, calcium binding motif, found in uncharacterized calglandulin-like proteins; The ...
 40-125 1.11e-03

EF-hand, calcium binding motif, found in uncharacterized calglandulin-like proteins; The family corresponds to a group of uncharacterized calglandulin-like proteins. Although their biological function remain unclear, they show high sequence similarity with human calglandulin-like protein GAGLP, which is an ortholog of calglandulin from the venom glands of Bothrops insularis snake. Both GAGLP and calglandulin are putative Ca2+-binding proteins with four EF-hand motifs. However, members in this family contain only three EF-hand motifs. In this point, they may belong to the parvalbumin-like EF-hand family, which is characterized by the presence of three consecutive EF-hand motifs (helix-loop-helix).


Pssm-ID: 319995 [Multi-domain]  Cd Length: 106  Bit Score: 35.97  E-value: 1.11e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2287722447  40 GPINFTMFLAMFGKKLNGTDPEDVIRNAFACFDEEATGTIQENYLRELLTMVGDR-----LADEEVDELYREAPIDKKGS 114
Cdd:cd16252    15 GSFNYSKFFEYMQKFQTSEQQEEAIRKAFQMLDKDKSGFIEWNEIKYILSTVPSSmpvapLSDEEAEAMIQAADTDGDGR 94

                          90
                  ....*....|.
gi 2287722447 115 FNYIQFTRILK 125
Cdd:cd16252    95 IDFQEFSDMVK 105
EFh_parvalbumin_like cd16251
EF-hand, calcium binding motif, found in parvalbumin-like EF-hand family; The family includes ...
 37-125 2.32e-03

EF-hand, calcium binding motif, found in parvalbumin-like EF-hand family; The family includes alpha- and beta-parvalbumins, and a group of uncharacterized calglandulin-like proteins. Parvalbumins are small, acidic, cytosolic EF-hand-containing Ca2+-buffer and Ca2+ transporter/shuttle proteins belonging to EF-hand superfamily. They are expressed by vertebrates in fast-twitch muscle cells, specific neurons of the central and peripheral nervous system, sensory cells of the mammalian auditory organ (Corti's cell), and some other cells, and characterized by the presence of three consecutive EF-hand motifs (helix-loop-helix) called AB, CD, and EF, but only CD and EF can chelate metal ions, such as Ca2+ and Mg2+. Thus, they may play an additional role in Mg2+ handling. Moreover, parvalbumins represent one of the major animal allergens. In metal-bound states, parvalbumins possess a rigid and stable tertiary structure and display strong allergenicity. In contrast, the metal-free parvalbumins are intrinsically disordered, and the loss of metal ions results in a conformational change that decreases their IgE binding capacity. Furthermore, parvalbumins have been widely used as a neuronal marker for a variety of functional brain systems. They also function as a Ca2+ shuttle transporting Ca2+ from troponin-C (TnC) to the sarcoplasmic reticulum (SR) Ca2+ pump during muscle relaxation. Thus they may facilitate myocardial relaxation and play important roles in cardiac diastolic dysfunction. Parvalbumins consists of alpha- and beta- sublineages, which can be distinguished on the basis of isoelectric point (pI > 5 for alpha; pI


Pssm-ID: 319994 [Multi-domain]  Cd Length: 101  Bit Score: 35.20  E-value: 2.32e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2287722447  37 EAAGPINFTMFLAMFGKKlngTDPEDVIRNAFACFDEEATGTIQENYLRELLTMV---GDRLADEEVDELYREAPIDKKG 113
Cdd:cd16251    12 RAHGSFNYKKFFEHVGLK---QKSEDQIKKVFQILDKDKSGFIEEEELKYILKGFsiaGRDLTDEETKALLAAGDTDGDG 88

                          90
                  ....*....|..
gi 2287722447 114 SFNYIQFTRILK 125
Cdd:cd16251    89 KIGVEEFATLVA 100
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 64-125 7.03e-03

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 32.90  E-value: 7.03e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2287722447  64 IRNAFACFDEEATGTIQENYLRELLTMVGDRLADEEVDELYREAPIDKKGSFNYIQFTRILK 125
Cdd:cd00051     2 LREAFRLFDKDGDGTISADELKAALKSLGEGLSEEEIDEMIREVDKDGDGKIDFEEFLELMA 63
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
1-114 7.32e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 34.38  E-value: 7.32e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2287722447   1 MIDQNRDGFIDKDDLHDMLASLgknpTDAYLDAMMNEAAGPINFTMFLAMFGKKLNGTDpEDVIRNAFACFDEEATGTIQ 80
Cdd:COG5126    13 LLDADGDGVLERDDFEALFRRL----WATLFSEADTDGDGRISREEFVAGMESLFEATV-EPFARAAFDLLDTDGDGKIS 87

                          90       100       110
                  ....*....|....*....|....*....|....
gi 2287722447  81 ENYLRELLTMVGdrLADEEVDELYREAPIDKKGS 114
Cdd:COG5126    88 ADEFRRLLTALG--VSEEEADELFARLDTDGDGK 119
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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