NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1370468924|ref|XP_024306136|]
View 

acyl-coenzyme A synthetase ACSM3, mitochondrial isoform X2 [Homo sapiens]

Protein Classification

acyl-CoA synthetase family protein; acyl--CoA ligase( domain architecture ID 10147736)

acyl-CoA synthetase family protein functions in fatty acid synthesis, and may catalyze the ATP-dependent activation of fatty acids in a two-step reaction to form acyl-CoA esters; belongs to the class I adenylate-forming enzyme superfamily; acyl--CoA ligase, belonging to the class I adenylate-forming enzyme family, catalyzes the formation of acyl-CoA from a carboxylic acid, CoA, and ATP; similar to Metallosphaera sedula 4-hydroxybutyrate--CoA ligase 1

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

Name Accession Description Interval E-value
MACS_euk cd05928
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ...
54-620 0e+00

Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.


:

Pssm-ID: 341251 [Multi-domain]  Cd Length: 530  Bit Score: 1092.12  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  54 IPEYFNFAKDVLDQWTDKEKAGKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEW 133
Cdd:cd05928     1 VPEYFNFASDVLDQWADKEKAGKRPPNPALWWVNGKGDEVKWSFRELGSLSRKAANVLSGACGLQRGDRVAVILPRVPEW 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 134 WLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIVSENSREGWGNLKELMk 213
Cdd:cd05928    81 WLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTSDELAPEVDSVASECPSLKTKLLVSEKSRDGWLNFKELL- 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 214 vflchpgwstvarswltatstslvqailsllsswdyRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGL 293
Cdd:cd05928   160 ------------------------------------NEASTEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGL 203
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 294 SVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN 373
Cdd:cd05928   204 KVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEPWIQGACVFVHHLPRFDPLVILKTLSSYPITTFCGAPTVYRMLVQQ 283
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 374 DITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNV 453
Cdd:cd05928   284 DLSSYKFPSLQHCVTGGEPLNPEVLEKWKAQTGLDIYEGYGQTETGLICANFKGMKIKPGSMGKASPPYDVQIIDDNGNV 363
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 454 LPPGQEGDIGIQVLPNRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENA 533
Cdd:cd05928   364 LPPGTEGDIGIRVKPIRPFGLFSGYVDNPEKTAATIRGDFYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESA 443
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 534 LNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRN 613
Cdd:cd05928   444 LIEHPAVVESAVVSSPDPIRGEVVKAFVVLAPQFLSHDPEQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRN 523

                  ....*..
gi 1370468924 614 ELRKKEW 620
Cdd:cd05928   524 ELRDKEW 530
 
Name Accession Description Interval E-value
MACS_euk cd05928
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ...
54-620 0e+00

Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.


Pssm-ID: 341251 [Multi-domain]  Cd Length: 530  Bit Score: 1092.12  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  54 IPEYFNFAKDVLDQWTDKEKAGKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEW 133
Cdd:cd05928     1 VPEYFNFASDVLDQWADKEKAGKRPPNPALWWVNGKGDEVKWSFRELGSLSRKAANVLSGACGLQRGDRVAVILPRVPEW 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 134 WLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIVSENSREGWGNLKELMk 213
Cdd:cd05928    81 WLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTSDELAPEVDSVASECPSLKTKLLVSEKSRDGWLNFKELL- 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 214 vflchpgwstvarswltatstslvqailsllsswdyRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGL 293
Cdd:cd05928   160 ------------------------------------NEASTEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGL 203
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 294 SVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN 373
Cdd:cd05928   204 KVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEPWIQGACVFVHHLPRFDPLVILKTLSSYPITTFCGAPTVYRMLVQQ 283
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 374 DITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNV 453
Cdd:cd05928   284 DLSSYKFPSLQHCVTGGEPLNPEVLEKWKAQTGLDIYEGYGQTETGLICANFKGMKIKPGSMGKASPPYDVQIIDDNGNV 363
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 454 LPPGQEGDIGIQVLPNRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENA 533
Cdd:cd05928   364 LPPGTEGDIGIRVKPIRPFGLFSGYVDNPEKTAATIRGDFYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESA 443
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 534 LNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRN 613
Cdd:cd05928   444 LIEHPAVVESAVVSSPDPIRGEVVKAFVVLAPQFLSHDPEQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRN 523

                  ....*..
gi 1370468924 614 ELRKKEW 620
Cdd:cd05928   524 ELRDKEW 530
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
54-620 0e+00

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 583.23  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  54 IPEYFNFAKDVLDQWtdkekAGKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEW 133
Cdd:COG0365     4 VGGRLNIAYNCLDRH-----AEGRGDKVALIWEGEDGEERTLTYAELRREVNRFANAL-RALGVKKGDRVAIYLPNIPEA 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 134 WLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDV---------LAPAVDAVASKCENLHSKLIV----SEN 200
Cdd:COG0365    78 VIAMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGglrggkvidLKEKVDEALEELPSLEHVIVVgrtgADV 157
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 201 SREGWGNLKELMKvflchpgwstvarswltatstslvqailsllsswdyrHASDSHTCVKTKHNEIMAIFFTSGTSGYPK 280
Cdd:COG0365   158 PMEGDLDWDELLA-------------------------------------AASAEFEPEPTDADDPLFILYTSGTTGKPK 200
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 281 MTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTHH-LPRF-EPTSILQTLSKYPIT 358
Cdd:COG0365   201 GVVHTHGGYLVHAATTAKYVLDLKPGDVFWCTADIGWATGHSYIVYGPLLNGATVVLYEgRPDFpDPGRLWELIEKYGVT 280
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 359 VFCSAPTVYRMLVQND---ITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETV-LICGNFKGMKIKPGS 434
Cdd:COG0365   281 VFFTAPTAIRALMKAGdepLKKYDLSSLRLLGSAGEPLNPEVWEWWYEAVGVPIVDGWGQTETGgIFISNLPGLPVKPGS 360
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 435 MGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvlpnRPF-GLFTHYVDNPSKTASTLRGNF---YITGDRGYMDKDGYFWF 510
Cdd:COG0365   361 MGKPVPGYDVAVVDEDGNPVPPGEEGELVIK----GPWpGMFRGYWNDPERYRETYFGRFpgwYRTGDGARRDEDGYFWI 436
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 511 VARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDqeQLIKEIQEHVKKTTAPY 590
Cdd:COG0365   437 LGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQVVKAFVVLKPGVEPSD--ELAKELQAHVREELGPY 514
                         570       580       590
                  ....*....|....*....|....*....|
gi 1370468924 591 KYPRKVEFIQELPKTISGKTKRNELRKKEW 620
Cdd:COG0365   515 AYPREIEFVDELPKTRSGKIMRRLLRKIAE 544
PRK04319 PRK04319
acetyl-CoA synthetase; Provisional
35-619 3.88e-133

acetyl-CoA synthetase; Provisional


Pssm-ID: 235279 [Multi-domain]  Cd Length: 570  Bit Score: 401.58  E-value: 3.88e-133
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  35 ATPQNFSnYESMKQDFKLGIPEYFNFAKDVLDQWTDKEKAGKkpsnPAFWWINRNGEEmRWSFEELGSLSRKFANILSEA 114
Cdd:PRK04319   20 ETYATFS-WEEVEKEFSWLETGKVNIAYEAIDRHADGGRKDK----VALRYLDASRKE-KYTYKELKELSNKFANVLKEL 93
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 115 cSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVdaVASKCENLHSK 194
Cdd:PRK04319   94 -GVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLITTPALLERK--PADDLPSLKHV 170
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 195 LIVSENSREGWG--NLKELMKvflchpgwstvarswltatstslvqailsllsswdyrHASDSHTCVKTKHNEIMAIFFT 272
Cdd:PRK04319  171 LLVGEDVEEGPGtlDFNALME-------------------------------------QASDEFDIEWTDREDGAILHYT 213
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 273 SGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGA--CVFThhlPRFEPTSILQ 350
Cdd:PRK04319  214 SGSTGKPKGVLHVHNAM-LQHYQTGKYVLDLHEDDVYWCTADPGWVTGTSYGIFAPWLNGAtnVIDG---GRFSPERWYR 289
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 351 TLSKYPITVFCSAPTVYRMLVQND---ITSYKFKSLKHCVSAGEPITPDVTeKWRNKT-GLDIYEGYGQTET--VLICgN 424
Cdd:PRK04319  290 ILEDYKVTVWYTAPTAIRMLMGAGddlVKKYDLSSLRHILSVGEPLNPEVV-RWGMKVfGLPIHDNWWMTETggIMIA-N 367
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 425 FKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvlPNRPfGLFTHYVDNPSKTASTLRGNFYITGDRGYMDK 504
Cdd:PRK04319  368 YPAMDIKPGSMGKPLPGIEAAIVDDQGNELPPNRMGNLAIK--KGWP-SMMRGIWNNPEKYESYFAGDWYVSGDSAYMDE 444
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 505 DGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKshDQEQLIKEIQEHVK 584
Cdd:PRK04319  445 DGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYE--PSEELKEEIRGFVK 522
                         570       580       590
                  ....*....|....*....|....*....|....*
gi 1370468924 585 KTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKE 619
Cdd:PRK04319  523 KGLGAHAAPREIEFKDKLPKTRSGKIMRRVLKAWE 557
benz_CoA_lig TIGR02262
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ...
80-617 8.35e-74

benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.


Pssm-ID: 274059 [Multi-domain]  Cd Length: 505  Bit Score: 245.52  E-value: 8.35e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  80 NPAFWWINRNGEEMR------------WSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVL 147
Cdd:TIGR02262   4 NAAEDLLDRNVVEGRggktafiddissLSYGELEAQVRRLAAALR-RLGVKREERVLLLMLDGVDFPIAFLGAIRAGIVP 82
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 148 IPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSkLIVSENSREGWGNLKELMkvflchpgwstvars 227
Cdd:TIGR02262  83 VALNTLLTADDYAYMLEDSRARVVFVSGALLPVIKAALGKSPHLEH-RVVVGRPEAGEVQLAELL--------------- 146
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 228 wltATStslvqailsllsswdyrhaSDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSD 307
Cdd:TIGR02262 147 ---ATE-------------------SEQFKPAATQADDPAFWLYSSGSTGMPKGVVHTHSNPYWTAELYARNTLGIREDD 204
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 308 VMWNTSDTGWAKSAWSSVFSPWIQGACVFTHHlPRFEPTSILQTLSKYPITVFCSAPTVYR-MLVQNDITSYKFKSLKHC 386
Cdd:TIGR02262 205 VCFSAAKLFFAYGLGNALTFPMSVGATTVLMG-ERPTPDAVFDRLRRHQPTIFYGVPTLYAaMLADPNLPSEDQVRLRLC 283
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 387 VSAGEPITPDVTEKWRNKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQV 466
Cdd:TIGR02262 284 TSAGEALPAEVGQRWQARFGVDIVDGIGSTEMLHIFLSNLPGDVRYGTSGKPVPGYRLRLVGDGGQDVADGEPGELLISG 363
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 467 lPNRPFGlfthYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVV 546
Cdd:TIGR02262 364 -PSSATM----YWNNRAKSRDTFQGEWTRSGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFEIESALIQHPAVLEAAVV 438
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1370468924 547 SSPDPIRGEVVKAFVVLNPDYKShdqeqLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:TIGR02262 439 GVADEDGLIKPKAFVVLRPGQTA-----LETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQRFKLRE 504
AMP-binding pfam00501
AMP-binding enzyme;
72-521 2.45e-71

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 236.05  E-value: 2.45e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  72 EKAGKKPSNPAFwwinRNGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGT 151
Cdd:pfam00501   3 RQAARTPDKTAL----EVGEGRRLTYRELDERANRLAAGL-RALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLN 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 152 TQLTQKDILYRLQSSKANCIITNDVL-APAVDAVASKCENLHSKLIVSENSREGWGNLKElmkvflchpgwstvarswlt 230
Cdd:pfam00501  78 PRLPAEELAYILEDSGAKVLITDDALkLEELLEALGKLEVVKLVLVLDRDPVLKEEPLPE-------------------- 137
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 231 atstslvqailsllssWDYRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTH---SSFGLGLSVNGRFWLDLTPSD 307
Cdd:pfam00501 138 ----------------EAKPADVPPPPPPPPDPDDLAYIIYTSGTTGKPKGVMLTHrnlVANVLSIKRVRPRGFGLGPDD 201
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 308 VMWNTSDTGWAKSAWSSVFSPWIQGA-CVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKH 385
Cdd:pfam00501 202 RVLSTLPLFHDFGLSLGLLGPLLAGAtVVLPPGFPALDPAALLELIERYKVTVLYGVPTLLNMLLEAgAPKRALLSSLRL 281
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 386 CVSAGEPITPDVTEKWRNKTGLDIYEGYGQTET---VLICGNFKGMKIKPGSMGKPSPAFDVKIVDVN-GNVLPPGQEGD 461
Cdd:pfam00501 282 VLSGGAPLPPELARRFRELFGGALVNGYGLTETtgvVTTPLPLDEDLRSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGE 361
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1370468924 462 I---GIQVLPnrpfGlfthYVDNPSKTASTL-RGNFYITGDRGYMDKDGYFWFVARADDVILSS 521
Cdd:pfam00501 362 LcvrGPGVMK----G----YLNDPELTAEAFdEDGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
 
Name Accession Description Interval E-value
MACS_euk cd05928
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ...
54-620 0e+00

Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.


Pssm-ID: 341251 [Multi-domain]  Cd Length: 530  Bit Score: 1092.12  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  54 IPEYFNFAKDVLDQWTDKEKAGKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEW 133
Cdd:cd05928     1 VPEYFNFASDVLDQWADKEKAGKRPPNPALWWVNGKGDEVKWSFRELGSLSRKAANVLSGACGLQRGDRVAVILPRVPEW 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 134 WLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIVSENSREGWGNLKELMk 213
Cdd:cd05928    81 WLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTSDELAPEVDSVASECPSLKTKLLVSEKSRDGWLNFKELL- 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 214 vflchpgwstvarswltatstslvqailsllsswdyRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGL 293
Cdd:cd05928   160 ------------------------------------NEASTEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGL 203
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 294 SVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN 373
Cdd:cd05928   204 KVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEPWIQGACVFVHHLPRFDPLVILKTLSSYPITTFCGAPTVYRMLVQQ 283
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 374 DITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNV 453
Cdd:cd05928   284 DLSSYKFPSLQHCVTGGEPLNPEVLEKWKAQTGLDIYEGYGQTETGLICANFKGMKIKPGSMGKASPPYDVQIIDDNGNV 363
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 454 LPPGQEGDIGIQVLPNRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENA 533
Cdd:cd05928   364 LPPGTEGDIGIRVKPIRPFGLFSGYVDNPEKTAATIRGDFYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESA 443
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 534 LNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRN 613
Cdd:cd05928   444 LIEHPAVVESAVVSSPDPIRGEVVKAFVVLAPQFLSHDPEQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRN 523

                  ....*..
gi 1370468924 614 ELRKKEW 620
Cdd:cd05928   524 ELRDKEW 530
MACS_like cd05972
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ...
95-617 0e+00

Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.


Pssm-ID: 341276 [Multi-domain]  Cd Length: 428  Bit Score: 614.35  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  95 WSFEELGSLSRKFANILSEACsLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITN 174
Cdd:cd05972     1 WSFRELKRESAKAANVLAKLG-LRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVTD 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 175 Dvlapavdavaskcenlhsklivsensregwgnlkelmkvflchpgwstvarswltatstslvqailsllsswdyrhasd 254
Cdd:cd05972    80 A------------------------------------------------------------------------------- 80
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 255 shtcvktkhNEIMAIFFTSGTSGYPKMTAHTHSsFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGAC 334
Cdd:cd05972    81 ---------EDPALIYFTSGTTGLPKGVLHTHS-YPLGHIPTAAYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGAT 150
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 335 VFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYG 414
Cdd:cd05972   151 VFVYEGPRFDAERILELLERYGVTSFCGPPTAYRMLIKQDLSSYKFSHLRLVVSAGEPLNPEVIEWWRAATGLPIRDGYG 230
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 415 QTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVLPNrpfGLFTHYVDNPSKTASTLRGNFY 494
Cdd:cd05972   231 QTETGLTVGNFPDMPVKPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKLPPP---GLFLGYVGDPEKTEASIRGDYY 307
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 495 ITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKshDQEQ 574
Cdd:cd05972   308 LTGDRAYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYE--PSEE 385
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|...
gi 1370468924 575 LIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05972   386 LAEELQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
54-620 0e+00

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 583.23  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  54 IPEYFNFAKDVLDQWtdkekAGKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEW 133
Cdd:COG0365     4 VGGRLNIAYNCLDRH-----AEGRGDKVALIWEGEDGEERTLTYAELRREVNRFANAL-RALGVKKGDRVAIYLPNIPEA 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 134 WLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDV---------LAPAVDAVASKCENLHSKLIV----SEN 200
Cdd:COG0365    78 VIAMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGglrggkvidLKEKVDEALEELPSLEHVIVVgrtgADV 157
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 201 SREGWGNLKELMKvflchpgwstvarswltatstslvqailsllsswdyrHASDSHTCVKTKHNEIMAIFFTSGTSGYPK 280
Cdd:COG0365   158 PMEGDLDWDELLA-------------------------------------AASAEFEPEPTDADDPLFILYTSGTTGKPK 200
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 281 MTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTHH-LPRF-EPTSILQTLSKYPIT 358
Cdd:COG0365   201 GVVHTHGGYLVHAATTAKYVLDLKPGDVFWCTADIGWATGHSYIVYGPLLNGATVVLYEgRPDFpDPGRLWELIEKYGVT 280
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 359 VFCSAPTVYRMLVQND---ITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETV-LICGNFKGMKIKPGS 434
Cdd:COG0365   281 VFFTAPTAIRALMKAGdepLKKYDLSSLRLLGSAGEPLNPEVWEWWYEAVGVPIVDGWGQTETGgIFISNLPGLPVKPGS 360
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 435 MGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvlpnRPF-GLFTHYVDNPSKTASTLRGNF---YITGDRGYMDKDGYFWF 510
Cdd:COG0365   361 MGKPVPGYDVAVVDEDGNPVPPGEEGELVIK----GPWpGMFRGYWNDPERYRETYFGRFpgwYRTGDGARRDEDGYFWI 436
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 511 VARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDqeQLIKEIQEHVKKTTAPY 590
Cdd:COG0365   437 LGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQVVKAFVVLKPGVEPSD--ELAKELQAHVREELGPY 514
                         570       580       590
                  ....*....|....*....|....*....|
gi 1370468924 591 KYPRKVEFIQELPKTISGKTKRNELRKKEW 620
Cdd:COG0365   515 AYPREIEFVDELPKTRSGKIMRRLLRKIAE 544
MACS_AAE_MA_like cd05970
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ...
43-619 0e+00

Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.


Pssm-ID: 341274 [Multi-domain]  Cd Length: 537  Bit Score: 580.22  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  43 YESMKQDFKLGIPEYFNFAKDVLDQWtdkekAGKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANILsEACSLQRGDR 122
Cdd:cd05970     1 YEDFHNNFSINVPENFNFAYDVVDAM-----AKEYPDKLALVWCDDAGEERIFTFAELADYSDKTANFF-KAMGIGKGDT 74
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 123 VILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSS--KANCIITNDVLAPAVDAVASKCENLHSKLIVSEN 200
Cdd:cd05970    75 VMLTLKRRYEFWYSLLALHKLGAIAIPATHQLTAKDIVYRIESAdiKMIVAIAEDNIPEEIEKAAPECPSKPKLVWVGDP 154
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 201 SREGWGNLKELMKVflchpgwstvarswltatstslvqailsllSSWDYRHasdSHTCVKTKHNEIMAIFFTSGTSGYPK 280
Cdd:cd05970   155 VPEGWIDFRKLIKN------------------------------ASPDFER---PTANSYPCGEDILLVYFSSGTTGMPK 201
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 281 MTAHTHSsFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTHHLPRFEPTSILQTLSKYPITVF 360
Cdd:cd05970   202 MVEHDFT-YPLGHIVTAKYWQNVREGGLHLTVADTGWGKAVWGKIYGQWIAGAAVFVYDYDKFDPKALLEKLSKYGVTTF 280
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 361 CSAPTVYRMLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKPSP 440
Cdd:cd05970   281 CAPPTIYRFLIREDLSRYDLSSLRYCTTAGEALNPEVFNTFKEKTGIKLMEGFGQTETTLTIATFPWMEPKPGSMGKPAP 360
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 441 AFDVKIVDVNGNVLPPGQEGDIGIQVLPNRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILS 520
Cdd:cd05970   361 GYEIDLIDREGRSCEAGEEGEIVIRTSKGKPVGLFGGYYKDAEKTAEVWHDGYYHTGDAAWMDEDGYLWFVGRTDDLIKS 440
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 521 SGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKShdQEQLIKEIQEHVKKTTAPYKYPRKVEFIQ 600
Cdd:cd05970   441 SGYRIGPFEVESALIQHPAVLECAVTGVPDPIRGQVVKATIVLAKGYEP--SEELKKELQDHVKKVTAPYKYPRIVEFVD 518
                         570
                  ....*....|....*....
gi 1370468924 601 ELPKTISGKTKRNELRKKE 619
Cdd:cd05970   519 ELPKTISGKIRRVEIRERD 537
MACS_like_1 cd05974
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
96-619 2.33e-137

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341278 [Multi-domain]  Cd Length: 432  Bit Score: 407.72  E-value: 2.33e-137
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKAnciitnd 175
Cdd:cd05974     2 SFAEMSARSSRVANFL-RSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDRVDRGGA------- 73
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 VLAPAVDAVaskcenlhsklivsensregwgnlkelmkvflchpgwstvarswltatstslvqailsllsswdyrHASDS 255
Cdd:cd05974    74 VYAAVDENT------------------------------------------------------------------HADDP 87
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 256 htcvktkhneiMAIFFTSGTSGYPKMTAHTHSSFGLGlSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACV 335
Cdd:cd05974    88 -----------MLLYFTSGTTSKPKLVEHTHRSYPVG-HLSTMYWIGLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATV 155
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 336 FTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFKsLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQ 415
Cdd:cd05974   156 FLFNYARFDAKRVLAALVRYGVTTLCAPPTVWRMLIQQDLASFDVK-LREVVGAGEPLNPEVIEQVRRAWGLTIRDGYGQ 234
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 416 TETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNvlpPGQEGDIGIQVLPNRPFGLFTHYVDNPSKTASTLRGNFYI 495
Cdd:cd05974   235 TETTALVGNSPGQPVKAGSMGRPLPGYRVALLDPDGA---PATEGEVALDLGDTRPVGLMKGYAGDPDKTAHAMRGGYYR 311
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 496 TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKshDQEQL 575
Cdd:cd05974   312 TGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYE--PSPET 389
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 1370468924 576 IKEIQEHVKKTTAPYKYPRKVEFIqELPKTISGKTKRNELRKKE 619
Cdd:cd05974   390 ALEIFRFSRERLAPYKRIRRLEFA-ELPKTISGKIRRVELRRRE 432
PRK04319 PRK04319
acetyl-CoA synthetase; Provisional
35-619 3.88e-133

acetyl-CoA synthetase; Provisional


Pssm-ID: 235279 [Multi-domain]  Cd Length: 570  Bit Score: 401.58  E-value: 3.88e-133
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  35 ATPQNFSnYESMKQDFKLGIPEYFNFAKDVLDQWTDKEKAGKkpsnPAFWWINRNGEEmRWSFEELGSLSRKFANILSEA 114
Cdd:PRK04319   20 ETYATFS-WEEVEKEFSWLETGKVNIAYEAIDRHADGGRKDK----VALRYLDASRKE-KYTYKELKELSNKFANVLKEL 93
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 115 cSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVdaVASKCENLHSK 194
Cdd:PRK04319   94 -GVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLITTPALLERK--PADDLPSLKHV 170
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 195 LIVSENSREGWG--NLKELMKvflchpgwstvarswltatstslvqailsllsswdyrHASDSHTCVKTKHNEIMAIFFT 272
Cdd:PRK04319  171 LLVGEDVEEGPGtlDFNALME-------------------------------------QASDEFDIEWTDREDGAILHYT 213
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 273 SGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGA--CVFThhlPRFEPTSILQ 350
Cdd:PRK04319  214 SGSTGKPKGVLHVHNAM-LQHYQTGKYVLDLHEDDVYWCTADPGWVTGTSYGIFAPWLNGAtnVIDG---GRFSPERWYR 289
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 351 TLSKYPITVFCSAPTVYRMLVQND---ITSYKFKSLKHCVSAGEPITPDVTeKWRNKT-GLDIYEGYGQTET--VLICgN 424
Cdd:PRK04319  290 ILEDYKVTVWYTAPTAIRMLMGAGddlVKKYDLSSLRHILSVGEPLNPEVV-RWGMKVfGLPIHDNWWMTETggIMIA-N 367
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 425 FKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvlPNRPfGLFTHYVDNPSKTASTLRGNFYITGDRGYMDK 504
Cdd:PRK04319  368 YPAMDIKPGSMGKPLPGIEAAIVDDQGNELPPNRMGNLAIK--KGWP-SMMRGIWNNPEKYESYFAGDWYVSGDSAYMDE 444
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 505 DGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKshDQEQLIKEIQEHVK 584
Cdd:PRK04319  445 DGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYE--PSEELKEEIRGFVK 522
                         570       580       590
                  ....*....|....*....|....*....|....*
gi 1370468924 585 KTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKE 619
Cdd:PRK04319  523 KGLGAHAAPREIEFKDKLPKTRSGKIMRRVLKAWE 557
MACS_like_4 cd05969
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ...
95-619 1.66e-122

Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.


Pssm-ID: 341273 [Multi-domain]  Cd Length: 442  Bit Score: 369.91  E-value: 1.66e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  95 WSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITN 174
Cdd:cd05969     1 YTFAQLKVLSARFANVL-KSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITT 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 175 DVLAPavdavaskcenlhsklivsensregwgnlkelmkvflchpgwstvarswltatstslvqailsllsswdyrhasd 254
Cdd:cd05969    80 EELYE--------------------------------------------------------------------------- 84
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 255 shtcvKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGAC 334
Cdd:cd05969    85 -----RTDPEDPTLLHYTSGTTGTPKGVLHVHDAM-IFYYFTGKYVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNGVT 158
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 335 VFTHHlPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDI---TSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYE 411
Cdd:cd05969   159 NVVYE-GRFDAESWYGIIERVKVTVWYTAPTAIRMLMKEGDelaRKYDLSSLRFIHSVGEPLNPEAIRWGMEVFGVPIHD 237
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 412 GYGQTET--VLICgNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvlPNRPfGLFTHYVDNPSKTASTL 489
Cdd:cd05969   238 TWWQTETgsIMIA-NYPCMPIKPGSMGKPLPGVKAAVVDENGNELPPGTKGILALK--PGWP-SMFRGIWNDEERYKNSF 313
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 490 RGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKS 569
Cdd:cd05969   314 IDGWYLTGDLAYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEP 393
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|
gi 1370468924 570 hdQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKE 619
Cdd:cd05969   394 --SDELKEEIINFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVLKAKE 441
MenE/FadK COG0318
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ...
63-621 1.52e-118

O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440087 [Multi-domain]  Cd Length: 452  Bit Score: 359.89  E-value: 1.52e-118
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  63 DVLDQWtdkekAGKKPSNPAFWWINRngeemRWSFEELGSLSRKFANILSEACsLQRGDRVILILPRVPEWWLANVACLR 142
Cdd:COG0318     3 DLLRRA-----AARHPDRPALVFGGR-----RLTYAELDARARRLAAALRALG-VGPGDRVALLLPNSPEFVVAFLAALR 71
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 143 TGTVLIPGTTQLTQKDILYRLQSSKANCIITndvlapavdavaskcenlhsklivsensregwgnlkelmkvflchpgws 222
Cdd:COG0318    72 AGAVVVPLNPRLTAEELAYILEDSGARALVT------------------------------------------------- 102
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 223 tvarswltatstslvqailsllsswdyrhasdshtcvktkhneiMAIFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLD 302
Cdd:COG0318   103 --------------------------------------------ALILYTSGTTGRPKGVMLTHRNL-LANAAAIAAALG 137
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 303 LTPSDVMWNTS----DTGWaksaWSSVFSPWIQGACVftHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITS 377
Cdd:COG0318   138 LTPGDVVLVALplfhVFGL----TVGLLAPLLAGATL--VLLPRFDPERVLELIERERVTVLFGVPTMLARLLRHpEFAR 211
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 378 YKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTET-VLICGNFKGMK-IKPGSMGKPSPAFDVKIVDVNGNVLP 455
Cdd:COG0318   212 YDLSSLRLVVSGGAPLPPELLERFEERFGVRIVEGYGLTETsPVVTVNPEDPGeRRPGSVGRPLPGVEVRIVDEDGRELP 291
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 456 PGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALN 535
Cdd:COG0318   292 PGEVGEIVV-----RGPNVMKGYWNDPEATAEAFRDGWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLA 366
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 536 EHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyKSHDQEQLIKEIQEHVkkttAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:COG0318   367 AHPGVAEAAVVGVPDEKWGERVVAFVVLRPG-AELDAEELRAFLRERL----ARYKVPRRVEFVDELPRTASGKIDRRAL 441

                  ....*.
gi 1370468924 616 RKKEWK 621
Cdd:COG0318   442 RERYAA 447
MACS_like_3 cd05971
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
89-616 5.69e-115

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341275 [Multi-domain]  Cd Length: 439  Bit Score: 350.58  E-value: 5.69e-115
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  89 NGEEMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKA 168
Cdd:cd05971     1 KGTPEKVTFKELKTASNRFANVLKEI-GLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGA 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 169 NCIITNDVLAPAVdavaskcenlhsklivsensregwgnlkelmkvflchpgwstvarswltatstslvqailsllsswd 248
Cdd:cd05971    80 SALVTDGSDDPAL------------------------------------------------------------------- 92
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 249 yrhasdshtcvktkhneimaIFFTSGTSGYPKMTAHTHSsFGLGLSVNGRFWLDLTP--SDVMWNTSDTGWAKSAWSSVF 326
Cdd:cd05971    93 --------------------IIYTSGTTGPPKGALHAHR-VLLGHLPGVQFPFNLFPrdGDLYWTPADWAWIGGLLDVLL 151
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 327 SPWIQGACVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRML-VQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKT 405
Cdd:cd05971   152 PSLYFGVPVLAHRMTKFDPKAALDLMSRYGVTTAFLPPTALKMMrQQGEQLKHAQVKLRAIATGGESLGEELLGWAREQF 231
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 406 GLDIYEGYGQTETVLICGNFKG-MKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvLPNrPFGLFThYVDNPSK 484
Cdd:cd05971   232 GVEVNEFYGQTECNLVIGNCSAlFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEIAVE-LPD-PVAFLG-YWNNPSA 308
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 485 TASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLN 564
Cdd:cd05971   309 TEKKMAGDWLLTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLN 388
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1370468924 565 PDYKshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:cd05971   389 PGET--PSDALAREIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELR 438
MACS_like_2 cd05973
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
96-617 3.51e-105

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341277 [Multi-domain]  Cd Length: 437  Bit Score: 325.24  E-value: 3.51e-105
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITND 175
Cdd:cd05973     2 TFGELRALSARFANALQEL-GVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVTDA 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 vlapavdavaskcenlhsklivsensregwgnlkelmkvflchpgwstvarswltatstslvqailsllsswDYRHASDS 255
Cdd:cd05973    81 ------------------------------------------------------------------------ANRHKLDS 88
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 256 HTCVktkhneimaIFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACV 335
Cdd:cd05973    89 DPFV---------MMFTSGTTGLPKGVPVPLRAL-AAFGAYLRDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPT 158
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 336 FTHHLPrFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFK--SLKHCVSAGEPITPDVTEKWRNKTGLDIYEGY 413
Cdd:cd05973   159 ILLEGG-FSVESTWRVIERLGVTNLAGSPTAYRLLMAAGAEVPARPkgRLRRVSSAGEPLTPEVIRWFDAALGVPIHDHY 237
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 414 GQTETVLICGNFKGMK--IKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVlPNRPFGLFTHYVDNPSKTAStlrG 491
Cdd:cd05973   238 GQTELGMVLANHHALEhpVHAGSAGRAMPGWRVAVLDDDGDELGPGEPGRLAIDI-ANSPLMWFRGYQLPDTPAID---G 313
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 492 NFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKShd 571
Cdd:cd05973   314 GYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEG-- 391
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*.
gi 1370468924 572 QEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05973   392 TPALADELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLRR 437
AFD_class_I cd04433
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ...
265-609 9.66e-105

Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341228 [Multi-domain]  Cd Length: 336  Bit Score: 320.39  E-value: 9.66e-105
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 265 EIMAIFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSDTGWAkSAWSSVFSPWIQGACVFTHhlPRFE 344
Cdd:cd04433     1 DPALILYTSGTTGKPKGVVLSHRNL-LAAAAALAASGGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLL--PKFD 76
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 345 PTSILQTLSKYPITVFCSAPTVYRMLVQND-ITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTET--VLI 421
Cdd:cd04433    77 PEAALELIEREKVTILLGVPTLLARLLKAPeSAGYDLSSLRALVSGGAPLPPELLERFEEAPGIKLVNGYGLTETggTVA 156
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 422 CGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvLPNRPFGlfthYVDNPSKTASTLRGNFYITGDRGY 501
Cdd:cd04433   157 TGPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVR-GPSVMKG----YWNNPEATAAVDEDGWYRTGDLGR 231
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 502 MDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPdykshDQEQLIKEIQE 581
Cdd:cd04433   232 LDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRP-----GADLDAEELRA 306
                         330       340
                  ....*....|....*....|....*...
gi 1370468924 582 HVKKTTAPYKYPRKVEFIQELPKTISGK 609
Cdd:cd04433   307 HVRERLAPYKVPRRVVFVDALPRTASGK 334
FC-FACS_FadD_like cd05936
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ...
63-616 3.85e-103

Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341259 [Multi-domain]  Cd Length: 468  Bit Score: 320.66  E-value: 3.85e-103
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  63 DVLDqwtdkEKAGKKPSNPAFWWINRngeemRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLR 142
Cdd:cd05936     3 DLLE-----EAARRFPDKTALIFMGR-----KLTYRELDALAEAFAAGLQNL-GVQPGDRVALMLPNCPQFPIAYFGALK 71
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 143 TGTVLIPGTTQLTQKDILYRLQSSKANCIItndvlapavdavaskcenlhsklivsensregwgnlkelmkvflchpgws 222
Cdd:cd05936    72 AGAVVVPLNPLYTPRELEHILNDSGAKALI-------------------------------------------------- 101
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 223 tVARSWLTATSTslvqaiLSLLSSWDYRHASDshtcvktkhneIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNgRFWL- 301
Cdd:cd05936   102 -VAVSFTDLLAA------GAPLGERVALTPED-----------VAVLQYTSGTTGVPKGAMLTHRNLVANALQI-KAWLe 162
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 302 -DLTPSDVMWNT-------SDTgwaksawSSVFSPWIQGACVFThhLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN 373
Cdd:cd05936   163 dLLEGDDVVLAAlplfhvfGLT-------VALLLPLALGATIVL--IPRFRPIGVLKEIRKHRVTIFPGVPTMYIALLNA 233
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 374 -DITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETV-LICGNFKGMKIKPGSMGKPSPAFDVKIVDVNG 451
Cdd:cd05936   234 pEFKKRDFSSLRLCISGGAPLPVEVAERFEELTGVPIVEGYGLTETSpVVAVNPLDGPRKPGSIGIPLPGTEVKIVDDDG 313
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 452 NVLPPGQEGDI---GIQVlpnrpfglFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPF 528
Cdd:cd05936   314 EELPPGEVGELwvrGPQV--------MKGYWNRPEETAEAFVDGWLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPR 385
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 529 EVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDykshdqEQLIK-EIQEHVKKTTAPYKYPRKVEFIQELPKTIS 607
Cdd:cd05936   386 EVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFVVLKEG------ASLTEeEIIAFCREQLAGYKVPRQVEFRDELPKSAV 459

                  ....*....
gi 1370468924 608 GKTKRNELR 616
Cdd:cd05936   460 GKILRRELR 468
BCL_4HBCL cd05959
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ...
56-616 9.86e-101

Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.


Pssm-ID: 341269 [Multi-domain]  Cd Length: 508  Bit Score: 315.85  E-value: 9.86e-101
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  56 EYFNFAKDVLDQwTDKEKAGKkpsnPAFwwINRNGEemrWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWL 135
Cdd:cd05959     1 EKYNAATLVDLN-LNEGRGDK----TAF--IDDAGS---LTYAELEAEARRVAGALRAL-GVKREERVLLIMLDTVDFPT 69
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 136 ANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIVSENSREGWGnlkelmkvf 215
Cdd:cd05959    70 AFLGAIRAGIVPVPVNTLLTPDDYAYYLEDSRARVVVVSGELAPVLAAALTKSEHTLVVLIVSGGAGPEAG--------- 140
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 216 lchpgwstvaRSWLTAtstslvqailsllsswDYRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSV 295
Cdd:cd05959   141 ----------ALLLAE----------------LVAAEAEQLKPAATHADDPAFWLYSSGSTGRPKGVVHLHADIYWTAEL 194
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 296 NGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSP-WIQGACVFthhLP-RFEPTSILQTLSKYPITVFCSAPTVYR-MLVQ 372
Cdd:cd05959   195 YARNVLGIREDDVCFSAAKLFFAYGLGNSLTFPlSVGATTVL---MPeRPTPAAVFKRIRRYRPTVFFGVPTLYAaMLAA 271
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 373 NDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETVLI-CGNFKGmKIKPGSMGKPSPAFDVKIVDVNG 451
Cdd:cd05959   272 PNLPSRDLSSLRLCVSAGEALPAEVGERWKARFGLDILDGIGSTEMLHIfLSNRPG-RVRYGTTGKPVPGYEVELRDEDG 350
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 452 NVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVE 531
Cdd:cd05959   351 GDVADGEPGELYV-----RGPSSATMYWNNRDKTRDTFQGEWTRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVE 425
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 532 NALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTK 611
Cdd:cd05959   426 SALVQHPAVLEAAVVGVEDEDGLTKPKAFVVLRPGYE--DSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQ 503

                  ....*
gi 1370468924 612 RNELR 616
Cdd:cd05959   504 RFKLR 508
ABCL cd05958
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ...
92-616 6.89e-91

2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.


Pssm-ID: 341268 [Multi-domain]  Cd Length: 439  Bit Score: 288.22  E-value: 6.89e-91
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  92 EMRWSFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQsskaNCI 171
Cdd:cd05958     8 EREWTYRDLLALANRIANVLVGELGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILD----KAR 83
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 172 ITNDVLAPAVDAVaskcenlhsklivsensregwgnlkelmkvflchpgwstvarswltatstslvqailsllsswdyrh 251
Cdd:cd05958    84 ITVALCAHALTAS------------------------------------------------------------------- 96
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 252 asdshtcvktkhNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQ 331
Cdd:cd05958    97 ------------DDICILAFTSGTTGAPKATMHFHRDPLASADRYAVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGV 164
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 332 GACVFThhLPRFEPTSILQTLSKYPITVFCSAPTVYR-MLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIY 410
Cdd:cd05958   165 GASGVL--LEEATPDLLLSAIARYKPTVLFTAPTAYRaMLAHPDAAGPDLSSLRKCVSAGEALPAALHRAWKEATGIPII 242
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 411 EGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvlpnRPFGLftHYVDNPSKtASTLR 490
Cdd:cd05958   243 DGIGSTEMFHIFISARPGDARPGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAVR----GPTGC--RYLADKRQ-RTYVQ 315
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 491 GNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSh 570
Cdd:cd05958   316 GGWNITGDTYSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIP- 394
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*.
gi 1370468924 571 dQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:cd05958   395 -GPVLARELQDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
PRK06187 PRK06187
long-chain-fatty-acid--CoA ligase; Validated
62-621 2.00e-86

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235730 [Multi-domain]  Cd Length: 521  Bit Score: 278.99  E-value: 2.00e-86
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  62 KDVLDQWtdkekAGKKPSNPAFWWinrngEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACL 141
Cdd:PRK06187    9 GRILRHG-----ARKHPDKEAVYF-----DGRRTTYAELDERVNRLANAL-RALGVKKGDRVAVFDWNSHEYLEAYFAVP 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 142 RTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIVSENSREG----WGNLKELMKvflc 217
Cdd:PRK06187   78 KIGAVLHPINIRLKPEEIAYILNDAEDRVVLVDSEFVPLLAAILPQLPTVRTVIVEGDGPAAPlapeVGEYEELLA---- 153
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 218 hpgwstvarswltatstslvqailsllsswdyrHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFgLGLSVNG 297
Cdd:PRK06187  154 ---------------------------------AASDTFDFPDIDENDAAAMLYTSGTTGHPKGVVLSHRNL-FLHSLAV 199
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 298 RFWLDLTPSDV------MWNTSDTGWAksawssvFSPWIQGAcvfTHHLP-RFEPTSILQTLSKYPITVFCSAPTVYRML 370
Cdd:PRK06187  200 CAWLKLSRDDVylvivpMFHVHAWGLP-------YLALMAGA---KQVIPrRFDPENLLDLIETERVTFFFAVPTIWQML 269
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 371 VQNDITS-YKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETV-LICGNF-----KGMKIKPGSMGKPSPAFD 443
Cdd:PRK06187  270 LKAPRAYfVDFSSLRLVIYGGAALPPALLREFKEKFGIDLVQGYGMTETSpVVSVLPpedqlPGQWTKRRSAGRPLPGVE 349
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 444 VKIVDVNGNVLPPGqEGDIG-IQVL-PNRPFGlfthYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSS 521
Cdd:PRK06187  350 ARIVDDDGDELPPD-GGEVGeIIVRgPWLMQG----YWNRPEATAETIDGGWLHTGDVGYIDEDGYLYITDRIKDVIISG 424
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 522 GYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyKSHDQEQLIKEIQEHVkkttAPYKYPRKVEFIQE 601
Cdd:PRK06187  425 GENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPG-ATLDAKELRAFLRGRL----AKFKLPKRIAFVDE 499
                         570       580
                  ....*....|....*....|
gi 1370468924 602 LPKTISGKTKRNELRKKEWK 621
Cdd:PRK06187  500 LPRTSVGKILKRVLREQYAE 519
BCL_like cd05919
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ...
96-616 1.37e-77

Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.


Pssm-ID: 341243 [Multi-domain]  Cd Length: 436  Bit Score: 253.15  E-value: 1.37e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITND 175
Cdd:cd05919    12 TYGQLHDGANRLGSAL-RNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARLVVTSA 90
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 vlapavDAVASkcenlhsklivsensregwgnlkelmkvflchpgwstvarswltatstslvqailsllssWDYrhasds 255
Cdd:cd05919    91 ------DDIAY------------------------------------------------------------LLY------ 98
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 256 htcvktkhneimaiffTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACV 335
Cdd:cd05919    99 ----------------SSGTTGPPKGVMHAHRDPLLFADAMAREALGLTPGDRVFSSAKMFFGYGLGNSLWFPLAVGASA 162
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 336 FTHHLPRfEPTSILQTLSKYPITVFCSAPTVY-RMLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYG 414
Cdd:cd05919   163 VLNPGWP-TAERVLATLARFRPTVLYGVPTFYaNLLDSCAGSPDALRSLRLCVSAGEALPRGLGERWMEHFGGPILDGIG 241
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 415 QTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGNFY 494
Cdd:cd05919   242 ATEVGHIFLSNRPGAWRLGSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLV-----RGPSAAVGYWNNPEKSRATFNGGWY 316
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 495 ITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKShdQEQ 574
Cdd:cd05919   317 RTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAP--QES 394
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|..
gi 1370468924 575 LIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:cd05919   395 LARDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
PRK07656 PRK07656
long-chain-fatty-acid--CoA ligase; Validated
74-618 2.65e-77

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236072 [Multi-domain]  Cd Length: 513  Bit Score: 254.83  E-value: 2.65e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  74 AGKKPSNPAFWWinrngEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQ 153
Cdd:PRK07656   15 ARRFGDKEAYVF-----GDQRLTYAELNARVRRAAAAL-AALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTR 88
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 154 LTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIVSENSREGwgnLKELMKVFlchpgwstvaRSWLTATS 233
Cdd:PRK07656   89 YTADEAAYILARGDAKALFVLGLFLGVDYSATTRLPALEHVVICETEEDDP---HTEKMKTF----------TDFLAAGD 155
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 234 TSLVQAILsllsswdyrHASDshtcvktkhneIMAIFFTSGTSGYPK--MTAHTHSsfglgLSvNGRFW---LDLTPSD- 307
Cdd:PRK07656  156 PAERAPEV---------DPDD-----------VADILFTSGTTGRPKgaMLTHRQL-----LS-NAADWaeyLGLTEGDr 209
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 308 --------------VMWNTsdtgwaksawssvfsPWIQGACVFTHhlPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN 373
Cdd:PRK07656  210 ylaanpffhvfgykAGVNA---------------PLMRGATILPL--PVFDPDEVFRLIETERITVLPGPPTMYNSLLQH 272
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 374 -DITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIY-EGYGQTE---TVLICGNFKGMKIKPGSMGKPSPAFDVKIVD 448
Cdd:PRK07656  273 pDRSAEDLSSLRLAVTGAASMPVALLERFESELGVDIVlTGYGLSEasgVTTFNRLDDDRKTVAGTIGTAIAGVENKIVN 352
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 449 VNGNVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGNFYI-TGDRGYMDKDGYFWFVARADDVILSSGYRIGP 527
Cdd:PRK07656  353 ELGEEVPVGEVGELLV-----RGPNVMKGYYDDPEATAAAIDADGWLhTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYP 427
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 528 FEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyKSHDQEQLIKEIQEHVkkttAPYKYPRKVEFIQELPKTIS 607
Cdd:PRK07656  428 AEVEEVLYEHPAVAEAAVIGVPDERLGEVGKAYVVLKPG-AELTEEELIAYCREHL----AKYKVPRSIEFLDELPKNAT 502
                         570
                  ....*....|.
gi 1370468924 608 GKTKRNELRKK 618
Cdd:PRK07656  503 GKVLKRALREK 513
FACL_FadD13-like cd17631
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ...
74-609 2.26e-76

fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.


Pssm-ID: 341286 [Multi-domain]  Cd Length: 435  Bit Score: 249.83  E-value: 2.26e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  74 AGKKPSNPAFWWINRngeemRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQ 153
Cdd:cd17631     5 ARRHPDRTALVFGGR-----SLTYAELDERVNRLAHALRAL-GVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFR 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 154 LTQKDILYRLQSSKAnciitndvlapavdavaskcenlhsKLIVSENSRegwgnlkelmkvflchpgwstvarswltats 233
Cdd:cd17631    79 LTPPEVAYILADSGA-------------------------KVLFDDLAL------------------------------- 102
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 234 tslvqailsllsswdyrhasdshtcvktkhneimaIFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDV----- 308
Cdd:cd17631   103 -----------------------------------LMYTSGTTGRPKGAMLTHRNL-LWNAVNALAALDLGPDDVllvva 146
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 309 -MWNTSDTGwaksawssVFSPWIQGACVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHC 386
Cdd:cd17631   147 pLFHIGGLG--------VFTLPTLLRGGTVVILRKFDPETVLDLIERHRVTSFFLVPTMIQALLQHpRFATTDLSSLRAV 218
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 387 VSAGEPITPDVTEKWRnKTGLDIYEGYGQTETV-LICGNFKGMKI-KPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGI 464
Cdd:cd17631   219 IYGGAPMPERLLRALQ-ARGVKFVQGYGMTETSpGVTFLSPEDHRrKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVV 297
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 465 QVlPNrpfgLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESA 544
Cdd:cd17631   298 RG-PH----VMAGYWNRPEATAAAFRDGWFHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVA 372
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1370468924 545 VVSSPDPIRGEVVKAFVVLNPDyKSHDQEQLIkeiqEHVKKTTAPYKYPRKVEFIQELPKTISGK 609
Cdd:cd17631   373 VIGVPDEKWGEAVVAVVVPRPG-AELDEDELI----AHCRERLARYKIPKSVEFVDALPRNATGK 432
ACS cd05966
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ...
59-617 4.57e-76

Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.


Pssm-ID: 341270 [Multi-domain]  Cd Length: 608  Bit Score: 254.02  E-value: 4.57e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  59 NFAKDVLDQWtdkekAGKKPSNPAFWWINRNGEEMR-WSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLAN 137
Cdd:cd05966    53 NISYNCLDRH-----LKERGDKVAIIWEGDEPDQSRtITYRELLREVCRFANVLKSL-GVKKGDRVAIYMPMIPELVIAM 126
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 138 VACLRTG---TVLIPGttqLTQKDILYRLQSSKANCIITND---------VLAPAVDAVASKCENLHsKLIVSENSregw 205
Cdd:cd05966   127 LACARIGavhSVVFAG---FSAESLADRINDAQCKLVITADggyrggkviPLKEIVDEALEKCPSVE-KVLVVKRT---- 198
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 206 gNLKELMkvflcHPG----WSTVARSwltatstslvqailsllsswdyrhASDSHTCVKTKHNEIMAIFFTSGTSGYPKM 281
Cdd:cd05966   199 -GGEVPM-----TEGrdlwWHDLMAK------------------------QSPECEPEWMDSEDPLFILYTSGSTGKPKG 248
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 282 TAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFThhlprFE--PT--------SILQt 351
Cdd:cd05966   249 VVHTTGGYLLYAATTFKYVFDYHPDDIYWCTADIGWITGHSYIVYGPLANGATTVM-----FEgtPTypdpgrywDIVE- 322
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 352 lsKYPITVFCSAPTVYRMLVQ---NDITSYKFKSLKHCVSAGEPITPdvtEKWR---NKTG---LDIYEGYGQTETVLIC 422
Cdd:cd05966   323 --KHKVTIFYTAPTAIRALMKfgdEWVKKHDLSSLRVLGSVGEPINP---EAWMwyyEVIGkerCPIVDTWWQTETGGIM 397
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 423 -----GnfkGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvlpnRPFglfthyvdnPSkTASTLRGN----- 492
Cdd:cd05966   398 itplpG---ATPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIK----RPW---------PG-MARTIYGDherye 460
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 493 ---------FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVL 563
Cdd:cd05966   461 dtyfskfpgYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTL 540
                         570       580       590       600       610
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1370468924 564 NPDYKSHDqeQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05966   541 KDGEEPSD--ELRKELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKIMRRILRK 592
PRK08314 PRK08314
long-chain-fatty-acid--CoA ligase; Validated
78-620 5.01e-76

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236235 [Multi-domain]  Cd Length: 546  Bit Score: 252.19  E-value: 5.01e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  78 PSNPAFWWINRngeemRWSFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQK 157
Cdd:PRK08314   24 PDKTAIVFYGR-----AISYRELLEEAERLAGYLQQECGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPMNREE 98
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 158 DILYRLQSSKANCIITNDVLAPAVDAVASKCENLHskLIVSENSregwGNLKELMKVFLchPGWSTVARSwLTATSTSLV 237
Cdd:PRK08314   99 ELAHYVTDSGARVAIVGSELAPKVAPAVGNLRLRH--VIVAQYS----DYLPAEPEIAV--PAWLRAEPP-LQALAPGGV 169
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 238 QAILSLLSSwdyRHASDSHTcvkTKHNEIMAIFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSD--- 314
Cdd:PRK08314  170 VAWKEALAA---GLAPPPHT---AGPDDLAVLPYTSGTTGVPKGCMHTHRTV-MANAVGSVLWSNSTPESVVLAVLPlfh 242
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 315 -TGWAKSAWSSVFSpwiqGACVFThhLPRFEPTSILQTLSKYPITVFCSAPTvyrMLV----QNDITSYKFKSLKHCVSA 389
Cdd:PRK08314  243 vTGMVHSMNAPIYA----GATVVL--MPRWDREAAARLIERYRVTHWTNIPT---MVVdflaSPGLAERDLSSLRYIGGG 313
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 390 GEPITPDVTEKWRNKTGLDIYEGYGQTETV-LICGNFKGmKIKPGSMGKPSPAFDVKIVD-VNGNVLPPGQEGDI---GI 464
Cdd:PRK08314  314 GAAMPEAVAERLKELTGLDYVEGYGLTETMaQTHSNPPD-RPKLQCLGIPTFGVDARVIDpETLEELPPGEVGEIvvhGP 392
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 465 QVlpnrpfglFTHYVDNPSKTAS---TLRGN-FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSV 540
Cdd:PRK08314  393 QV--------FKGYWNRPEATAEafiEIDGKrFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAI 464
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 541 AESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQEQlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKEW 620
Cdd:PRK08314  465 QEACVIATPDPRRGETVKAVVVLRPEARGKTTEE---EIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKILWRQLQEQEK 541
ACS-like cd17634
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ...
95-609 3.33e-74

acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341289 [Multi-domain]  Cd Length: 587  Bit Score: 248.65  E-value: 3.33e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  95 WSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITN 174
Cdd:cd17634    85 ISYRELHREVCRFAGTL-LDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLLITA 163
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 175 D----------VLAPAVDAVASKCENLHSKLIVSensREGwgnlkelmkvflCHPGWSTVARSWLTAtstsLVQAilsll 244
Cdd:cd17634   164 DggvragrsvpLKKNVDDALNPNVTSVEHVIVLK---RTG------------SDIDWQEGRDLWWRD----LIAK----- 219
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 245 sswdyrhASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSS 324
Cdd:cd17634   220 -------ASPEHQPEAMNAEDPLFILYTSGTTGKPKGVLHTTGGYLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHSYL 292
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 325 VFSPWIQGACVFTHH-LPRF-EPTSILQTLSKYPITVFCSAPTVYRMLVQND---ITSYKFKSLKHCVSAGEPITPDVTE 399
Cdd:cd17634   293 LYGPLACGATTLLYEgVPNWpTPARMWQVVDKHGVNILYTAPTAIRALMAAGddaIEGTDRSSLRILGSVGEPINPEAYE 372
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 400 -KWR--NKTGLDIYEGYGQTETV-LICGNFKGM-KIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQV-LPNRPFG 473
Cdd:cd17634   373 wYWKkiGKEKCPVVDTWWQTETGgFMITPLPGAiELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDpWPGQTRT 452
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 474 LFThyvDNPSKTASTLR--GNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDP 551
Cdd:cd17634   453 LFG---DHERFEQTYFStfKGMYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHA 529
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1370468924 552 IRGEVVKAFVVLNPDYKshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGK 609
Cdd:cd17634   530 IKGQAPYAYVVLNHGVE--PSPELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGK 585
Firefly_Luc_like cd05911
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ...
95-609 3.73e-74

Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341237 [Multi-domain]  Cd Length: 486  Bit Score: 245.59  E-value: 3.73e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  95 WSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIIT- 173
Cdd:cd05911    11 LTYAQLRTLSRRLAAGLRKL-GLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIFTd 89
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 174 NDVLAPAVDAVASkcenlhsklivsensregwgnlkelmkvflchpgWSTVARSWLTATSTSLVQAILSLLSSWDYRHAS 253
Cdd:cd05911    90 PDGLEKVKEAAKE----------------------------------LGPKDKIIVLDDKPDGVLSIEDLLSPTLGEEDE 135
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 254 DSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGL-SVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWiQG 332
Cdd:cd05911   136 DLPPPLKDGKDDTAAILYSSGTTGLPKGVCLSHRNLIANLsQVQTFLYGNDGSNDVILGFLPLYHIYGLFTTLASLL-NG 214
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 333 ACVFTHhlPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGL-DIY 410
Cdd:cd05911   215 ATVIIM--PKFDSELFLDLIEKYKITFLYLVPPIAAALAKSpLLDKYDLSSLRVILSGGAPLSKELQELLAKRFPNaTIK 292
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 411 EGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGN-VLPPGQEGDI---GIQVLPnrpfGlfthYVDNPSKTA 486
Cdd:cd05911   293 QGYGMTETGGILTVNPDGDDKPGSVGRLLPNVEAKIVDDDGKdSLGPNEPGEIcvrGPQVMK----G----YYNNPEATK 364
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 487 STL-RGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNP 565
Cdd:cd05911   365 ETFdEDGWLHTGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKP 444
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*
gi 1370468924 566 DYKSHDqeqliKEIQEHVKKTTAPYKYPRK-VEFIQELPKTISGK 609
Cdd:cd05911   445 GEKLTE-----KEVKDYVAKKVASYKQLRGgVVFVDEIPKSASGK 484
benz_CoA_lig TIGR02262
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ...
80-617 8.35e-74

benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.


Pssm-ID: 274059 [Multi-domain]  Cd Length: 505  Bit Score: 245.52  E-value: 8.35e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  80 NPAFWWINRNGEEMR------------WSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVL 147
Cdd:TIGR02262   4 NAAEDLLDRNVVEGRggktafiddissLSYGELEAQVRRLAAALR-RLGVKREERVLLLMLDGVDFPIAFLGAIRAGIVP 82
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 148 IPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSkLIVSENSREGWGNLKELMkvflchpgwstvars 227
Cdd:TIGR02262  83 VALNTLLTADDYAYMLEDSRARVVFVSGALLPVIKAALGKSPHLEH-RVVVGRPEAGEVQLAELL--------------- 146
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 228 wltATStslvqailsllsswdyrhaSDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSD 307
Cdd:TIGR02262 147 ---ATE-------------------SEQFKPAATQADDPAFWLYSSGSTGMPKGVVHTHSNPYWTAELYARNTLGIREDD 204
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 308 VMWNTSDTGWAKSAWSSVFSPWIQGACVFTHHlPRFEPTSILQTLSKYPITVFCSAPTVYR-MLVQNDITSYKFKSLKHC 386
Cdd:TIGR02262 205 VCFSAAKLFFAYGLGNALTFPMSVGATTVLMG-ERPTPDAVFDRLRRHQPTIFYGVPTLYAaMLADPNLPSEDQVRLRLC 283
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 387 VSAGEPITPDVTEKWRNKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQV 466
Cdd:TIGR02262 284 TSAGEALPAEVGQRWQARFGVDIVDGIGSTEMLHIFLSNLPGDVRYGTSGKPVPGYRLRLVGDGGQDVADGEPGELLISG 363
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 467 lPNRPFGlfthYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVV 546
Cdd:TIGR02262 364 -PSSATM----YWNNRAKSRDTFQGEWTRSGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFEIESALIQHPAVLEAAVV 438
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1370468924 547 SSPDPIRGEVVKAFVVLNPDYKShdqeqLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:TIGR02262 439 GVADEDGLIKPKAFVVLRPGQTA-----LETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQRFKLRE 504
PrpE cd05967
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ...
90-617 5.26e-73

Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.


Pssm-ID: 341271 [Multi-domain]  Cd Length: 617  Bit Score: 246.07  E-value: 5.26e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  90 GEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTV--LIPG---TTQLTQkdilyRLQ 164
Cdd:cd05967    78 GTERTYTYAELLDEVSRLAGVL-RKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIhsVVFGgfaAKELAS-----RID 151
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 165 SSKANCIITNDV---------LAPAVDAVASKCENLHSKLIVSEnsregwgnlKELMKVFLCHPG----WSTVarswlta 231
Cdd:cd05967   152 DAKPKLIVTASCgiepgkvvpYKPLLDKALELSGHKPHHVLVLN---------RPQVPADLTKPGrdldWSEL------- 215
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 232 tstslvqailsllsswdyRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSDVMWN 311
Cdd:cd05967   216 ------------------LAKAEPVDCVPVAATDPLYILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIYGIKPGDVWWA 277
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 312 TSDTGWAKSAWSSVFSPWIQGA-CVFTHHLPRF--EPTSILQTLSKYPITVFCSAPTVYRMLVQND-----ITSYKFKSL 383
Cdd:cd05967   278 ASDVGWVVGHSYIVYGPLLHGAtTVLYEGKPVGtpDPGAFWRVIEKYQVNALFTAPTAIRAIRKEDpdgkyIKKYDLSSL 357
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 384 KHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTET----VLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQE 459
Cdd:cd05967   358 RTLFLAGERLDPPTLEWAENTLGVPVIDHWWQTETgwpiTANPVGLEPLPIKAGSPGKPVPGYQVQVLDEDGEPVGPNEL 437
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 460 GDIGIQvLPNRPFGLFTHYVDNP---SKTASTLRGnFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNE 536
Cdd:cd05967   438 GNIVIK-LPLPPGCLLTLWKNDErfkKLYLSKFPG-YYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLS 515
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 537 HPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKShDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:cd05967   516 HPAVAECAVVGVRDELKGQVPLGLVVLKEGVKI-TAEELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLR 594

                  .
gi 1370468924 617 K 617
Cdd:cd05967   595 K 595
AMP-binding pfam00501
AMP-binding enzyme;
72-521 2.45e-71

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 236.05  E-value: 2.45e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  72 EKAGKKPSNPAFwwinRNGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGT 151
Cdd:pfam00501   3 RQAARTPDKTAL----EVGEGRRLTYRELDERANRLAAGL-RALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLN 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 152 TQLTQKDILYRLQSSKANCIITNDVL-APAVDAVASKCENLHSKLIVSENSREGWGNLKElmkvflchpgwstvarswlt 230
Cdd:pfam00501  78 PRLPAEELAYILEDSGAKVLITDDALkLEELLEALGKLEVVKLVLVLDRDPVLKEEPLPE-------------------- 137
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 231 atstslvqailsllssWDYRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTH---SSFGLGLSVNGRFWLDLTPSD 307
Cdd:pfam00501 138 ----------------EAKPADVPPPPPPPPDPDDLAYIIYTSGTTGKPKGVMLTHrnlVANVLSIKRVRPRGFGLGPDD 201
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 308 VMWNTSDTGWAKSAWSSVFSPWIQGA-CVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKH 385
Cdd:pfam00501 202 RVLSTLPLFHDFGLSLGLLGPLLAGAtVVLPPGFPALDPAALLELIERYKVTVLYGVPTLLNMLLEAgAPKRALLSSLRL 281
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 386 CVSAGEPITPDVTEKWRNKTGLDIYEGYGQTET---VLICGNFKGMKIKPGSMGKPSPAFDVKIVDVN-GNVLPPGQEGD 461
Cdd:pfam00501 282 VLSGGAPLPPELARRFRELFGGALVNGYGLTETtgvVTTPLPLDEDLRSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGE 361
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1370468924 462 I---GIQVLPnrpfGlfthYVDNPSKTASTL-RGNFYITGDRGYMDKDGYFWFVARADDVILSS 521
Cdd:pfam00501 362 LcvrGPGVMK----G----YLNDPELTAEAFdEDGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
LC_FACS_like cd05935
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ...
96-615 5.08e-71

Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.


Pssm-ID: 341258 [Multi-domain]  Cd Length: 430  Bit Score: 235.84  E-value: 5.08e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITnd 175
Cdd:cd05935     3 TYLELLEVVKKLASFLS-NKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVV-- 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 vlapavdavaskcenlHSKLivsensregwgnlkelmkvflchpgwstvarswltatstslvqailsllsswdyrhasds 255
Cdd:cd05935    80 ----------------GSEL------------------------------------------------------------ 83
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 256 htcvktkhNEIMAIFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACV 335
Cdd:cd05935    84 --------DDLALIPYTSGTTGLPKGCMHTHFSA-AANALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTY 154
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 336 FThhLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYG 414
Cdd:cd05935   155 VL--MARWDRETALELIEKYKVTFWTNIPTMLVDLLATpEFKTRDLSSLKVLTGGGAPMPPAVAEKLLKLTGLRFVEGYG 232
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 415 QTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDV-NGNVLPPGQEGDIGIQVlPNrpfgLFTHYVDNPSKTAS---TLR 490
Cdd:cd05935   233 LTETMSQTHTNPPLRPKLQCLGIP*FGVDARVIDIeTGRELPPNEVGEIVVRG-PQ----IFKGYWNRPEETEEsfiEIK 307
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 491 GN-FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKS 569
Cdd:cd05935   308 GRrFFRTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYRG 387
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*.
gi 1370468924 570 HDQEQlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd05935   388 KVTEE---DIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
FACL_DitJ_like cd05934
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
268-616 7.35e-68

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.


Pssm-ID: 341257 [Multi-domain]  Cd Length: 422  Bit Score: 227.17  E-value: 7.35e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 268 AIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRfWLDLTPSDVMW--------NTSDTGWAkSAWSSvfspwiQGACVFthh 339
Cdd:cd05934    85 SILYTSGTTGPPKGVVITHANLTFAGYYSAR-RFGLGEDDVYLtvlplfhiNAQAVSVL-AALSV------GATLVL--- 153
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 340 LPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDItsyKFKSLKHCVSA--GEPITPDVTEKWRNKTGLDIYEGYGQTE 417
Cdd:cd05934   154 LPRFSASRFWSDVRRYGATVTNYLGAMLSYLLAQPP---SPDDRAHRLRAayGAPNPPELHEEFEERFGVRLLEGYGMTE 230
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 418 TVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvlPNRPFGLFTHYVDNPSKTASTLRGNFYITG 497
Cdd:cd05934   231 TIVGVIGPRDEPRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIR--GLRGWGFFKGYYNMPEATAEAMRNGWFHTG 308
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 498 DRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYK-SHDqeqli 576
Cdd:cd05934   309 DLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETlDPE----- 383
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|
gi 1370468924 577 kEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:cd05934   384 -ELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
PRK06710 PRK06710
long-chain-fatty-acid--CoA ligase; Validated
96-621 1.33e-65

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 180666 [Multi-domain]  Cd Length: 563  Bit Score: 224.91  E-value: 1.33e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITND 175
Cdd:PRK06710   51 TFSVFHDKVKRFANYL-QKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLYTERELEYQLHDSGAKVILCLD 129
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 VLAPAVDAVASKCENLHskLIVSEnsregwgnlkelMKVFLCHPgwSTVARSWLTATSTSLVQAILS--LLSSWDYRHaS 253
Cdd:PRK06710  130 LVFPRVTNVQSATKIEH--VIVTR------------IADFLPFP--KNLLYPFVQKKQSNLVVKVSEseTIHLWNSVE-K 192
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 254 DSHTCVKT---KHNEIMAIFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLD--LTPSDVMWNTSDTGWAKSAWSSVFSP 328
Cdd:PRK06710  193 EVNTGVEVpcdPENDLALLQYTGGTTGFPKGVMLTHKNL-VSNTLMGVQWLYncKEGEEVVLGVLPFFHVYGMTAVMNLS 271
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 329 WIQGACVFThhLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDI-TSYKFKSLKHCVSAGEPITPDVTEKWRNKTGL 407
Cdd:PRK06710  272 IMQGYKMVL--IPKFDMKMVFEAIKKHKVTLFPGAPTIYIALLNSPLlKEYDISSIRACISGSAPLPVEVQEKFETVTGG 349
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 408 DIYEGYGQTETVLIC-GNFKGMKIKPGSMGKPSPAFDVKIVDV-NGNVLPPGQEGDI---GIQVLPNrpfglfthYVDNP 482
Cdd:PRK06710  350 KLVEGYGLTESSPVThSNFLWEKRVPGSIGVPWPDTEAMIMSLeTGEALPPGEIGEIvvkGPQIMKG--------YWNKP 421
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 483 SKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVV 562
Cdd:PRK06710  422 EETAAVLQDGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVV 501
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1370468924 563 LNPDYKSHDQeqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKEWK 621
Cdd:PRK06710  502 LKEGTECSEE-----ELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLIEEEKR 555
PRK00174 PRK00174
acetyl-CoA synthetase; Provisional
106-617 3.37e-63

acetyl-CoA synthetase; Provisional


Pssm-ID: 234677 [Multi-domain]  Cd Length: 637  Bit Score: 220.01  E-value: 3.37e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 106 KFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTG---TVLIPGttqLTQKDILYRLQSSKANCIITND------- 175
Cdd:PRK00174  110 RFANALKSL-GVKKGDRVAIYMPMIPEAAVAMLACARIGavhSVVFGG---FSAEALADRIIDAGAKLVITADegvrggk 185
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 --VLAPAVDAVASKCENLHSKLIVS--------ENSREGWGNlkELMKvflchpgwstvarswltatstslvqailslls 245
Cdd:PRK00174  186 piPLKANVDEALANCPSVEKVIVVRrtggdvdwVEGRDLWWH--ELVA-------------------------------- 231
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 246 swdyrHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSV 325
Cdd:PRK00174  232 -----GASDECEPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAAMTMKYVFDYKDGDVYWCTADVGWVTGHSYIV 306
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 326 FSPWIQGACVFTHH-LPRF-EPTSILQTLSKYPITVFCSAPTVYRMLVQ---NDITSYKFKSLKHCVSAGEPITPdvtEK 400
Cdd:PRK00174  307 YGPLANGATTLMFEgVPNYpDPGRFWEVIDKHKVTIFYTAPTAIRALMKegdEHPKKYDLSSLRLLGSVGEPINP---EA 383
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 401 WR---NKTGLD---IYEGYGQTET--VLIC---GnfkGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIqvlpN 469
Cdd:PRK00174  384 WEwyyKVVGGErcpIVDTWWQTETggIMITplpG---ATPLKPGSATRPLPGIQPAVVDEEGNPLEGGEGGNLVI----K 456
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 470 RPFglfthyvdnPS-------------KTA-STLRGNfYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALN 535
Cdd:PRK00174  457 DPW---------PGmmrtiygdherfvKTYfSTFKGM-YFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALV 526
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 536 EHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDqeQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:PRK00174  527 AHPKVAEAAVVGRPDDIKGQGIYAFVTLKGGEEPSD--ELRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGKIMRRIL 604

                  ..
gi 1370468924 616 RK 617
Cdd:PRK00174  605 RK 606
AACS_like cd05968
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ...
59-616 1.40e-62

Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.


Pssm-ID: 341272 [Multi-domain]  Cd Length: 610  Bit Score: 218.13  E-value: 1.40e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  59 NFAKDVLDQWTdkekaGKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANV 138
Cdd:cd05968    61 NIVEQLLDKWL-----ADTRTRPALRWEGEDGTSRTLTYGELLYEVKRLANGL-RALGVGKGDRVGIYLPMIPEIVPAFL 134
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 139 ACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDV---------LAPAVDAVASKCENLHSKLIVSENSREgwgnlk 209
Cdd:cd05968   135 AVARIGGIVVPIFSGFGKEAAATRLQDAEAKALITADGftrrgrevnLKEEADKACAQCPTVEKVVVVRHLGND------ 208
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 210 elmkvflchPGWSTVARSWltatstslvqailsllssWDYRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSF 289
Cdd:cd05968   209 ---------FTPAKGRDLS------------------YDEEKETAGDGAERTESEDPLMIIYTSGTTGKPKGTVHVHAGF 261
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 290 GLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWsSVFSPWIQGACVFTHH-LPRF-EPTSILQTLSKYPITVFCSAPTVY 367
Cdd:cd05968   262 PLKAAQDMYFQFDLKPGDLLTWFTDLGWMMGPW-LIFGGLILGATMVLYDgAPDHpKADRLWRMVEDHEITHLGLSPTLI 340
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 368 RMLV---QNDITSYKFKSLKHCVSAGEPITPdvtEKW------RNKTGLDIYEGYGQTETV-LICGNFKGMKIKPGSMGK 437
Cdd:cd05968   341 RALKprgDAPVNAHDLSSLRVLGSTGEPWNP---EPWnwlfetVGKGRNPIINYSGGTEISgGILGNVLIKPIKPSSFNG 417
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 438 PSPAFDVKIVDVNGNVLPPgQEGDIGIQ-VLPNRPFGLF---THYVDnpskTASTLRGNFYITGDRGYMDKDGYFWFVAR 513
Cdd:cd05968   418 PVPGMKADVLDESGKPARP-EVGELVLLaPWPGMTRGFWrdeDRYLE----TYWSRFDNVWVHGDFAYYDEEGYFYILGR 492
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 514 ADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHD--QEQLIKEIQEHVKKttaPYK 591
Cdd:cd05968   493 SDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFVVLKPGVTPTEalAEELMERVADELGK---PLS 569
                         570       580
                  ....*....|....*....|....*
gi 1370468924 592 yPRKVEFIQELPKTISGKTKRNELR 616
Cdd:cd05968   570 -PERILFVKDLPKTRNAKVMRRVIR 593
FACL_fum10p_like cd05926
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ...
95-617 2.19e-62

Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.


Pssm-ID: 341249 [Multi-domain]  Cd Length: 493  Bit Score: 214.48  E-value: 2.19e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  95 WSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIIT- 173
Cdd:cd05926    15 LTYADLAELVDDLARQLAAL-GIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVLTp 93
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 174 NDVLAPAVDAVAskcenlhsklivsensregwgnlkelmkvflcHPGWSTVARSWLTATSTSLVQAilSLLSSWDYRHAS 253
Cdd:cd05926    94 KGELGPASRAAS--------------------------------KLGLAILELALDVGVLIRAPSA--ESLSNLLADKKN 139
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 254 DSHTCVKTKhNEIMAIFFTSGTSGYPKMTAHTHSSfgLGLSV-NGRFWLDLTPSD----VMWNTSDTGWAKSAWSSVFSp 328
Cdd:cd05926   140 AKSEGVPLP-DDLALILHTSGTTGRPKGVPLTHRN--LAASAtNITNTYKLTPDDrtlvVMPLFHVHGLVASLLSTLAA- 215
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 329 wiQGACVFThhlPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSY--KFKSLKHCVSAGEPITPDVTEKWRNKTG 406
Cdd:cd05926   216 --GGSVVLP---PRFSASTFWPDVRDYNATWYTAVPTIHQILLNRPEPNPesPPPKLRFIRSCSASLPPAVLEALEATFG 290
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 407 LDIYEGYGQTETV--LICGNFKGMKIKPGSMGKPSPAfDVKIVDVNGNVLPPGQEGDIGIQVlPNRPFGlfthYVDNPSK 484
Cdd:cd05926   291 APVLEAYGMTEAAhqMTSNPLPPGPRKPGSVGKPVGV-EVRILDEDGEILPPGVVGEICLRG-PNVTRG----YLNNPEA 364
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 485 TA-STLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVL 563
Cdd:cd05926   365 NAeAAFKDGWFRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVL 444
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1370468924 564 NPDYKShdqeqLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05926   445 REGASV-----TEEELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
MCS cd05941
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ...
268-617 1.53e-61

Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.


Pssm-ID: 341264 [Multi-domain]  Cd Length: 442  Bit Score: 210.99  E-value: 1.53e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 268 AIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWlDLTPSDV------------MWNtsdtgwaksawsSVFSPWIQGACV 335
Cdd:cd05941    93 LILYTSGTTGRPKGVVLTHANLAANVRALVDAW-RWTEDDVllhvlplhhvhgLVN------------ALLCPLFAGASV 159
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 336 ftHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQ---------NDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTG 406
Cdd:cd05941   160 --EFLPKFDPKEVAISRLMPSITVFMGVPTIYTRLLQyyeahftdpQFARAAAAERLRLMVSGSAALPVPTLEEWEAITG 237
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 407 LDIYEGYGQTETVLICGN-FKGmKIKPGSMGKPSPAFDVKIVDVNGNvlPPGQEGDIG-IQVlpnRPFGLFTHYVDNPSK 484
Cdd:cd05941   238 HTLLERYGMTEIGMALSNpLDG-ERRPGTVGMPLPGVQARIVDEETG--EPLPRGEVGeIQV---RGPSVFKEYWNKPEA 311
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 485 TASTLRG-NFYITGDRGYMDKDGYFWFVAR-ADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVV 562
Cdd:cd05941   312 TKEEFTDdGWFKTGDLGVVDEDGYYWILGRsSVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVV 391
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1370468924 563 LNPDYKSHDQEQLIkeiqEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05941   392 LRAGAAALSLEELK----EWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
PRK08316 PRK08316
acyl-CoA synthetase; Validated
91-617 1.57e-61

acyl-CoA synthetase; Validated


Pssm-ID: 181381 [Multi-domain]  Cd Length: 523  Bit Score: 212.87  E-value: 1.57e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  91 EEMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANC 170
Cdd:PRK08316   33 GDRSWTYAELDAAVNRVAAALLDL-GLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEELAYILDHSGARA 111
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 171 IITNDVLAPAVDAVASKCENLHSKLIVSENSREGWGnlkelmkvflchpGWSTVARsWLTATSTSLVQAILsllsswdyr 250
Cdd:PRK08316  112 FLVDPALAPTAEAALALLPVDTLILSLVLGGREAPG-------------GWLDFAD-WAEAGSVAEPDVEL--------- 168
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 251 hasdshtcvktkHNEIMA-IFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNtsdtgwA----KSAWSSV 325
Cdd:PRK08316  169 ------------ADDDLAqILYTSGTESLPKGAMLTHRAL-IAEYVSCIVAGDMSADDIPLH------AlplyHCAQLDV 229
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 326 F-SPWIQ-GACvfTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSaGEPITP-DVTEKW 401
Cdd:PRK08316  230 FlGPYLYvGAT--NVILDAPDPELILRTIEAERITSFFAPPTVWISLLRHpDFDTRDLSSLRKGYY-GASIMPvEVLKEL 306
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 402 RNK-TGLDIYEGYGQTE-----TVLicgNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIgiqvlPNRPFGLF 475
Cdd:PRK08316  307 RERlPGLRFYNCYGQTEiaplaTVL---GPEEHLRRPGSAGRPVLNVETRVVDDDGNDVAPGEVGEI-----VHRSPQLM 378
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 476 THYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGE 555
Cdd:PRK08316  379 LGYWDDPEKTAEAFRGGWFHSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIE 458
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1370468924 556 VVKAFVVLNPDyKSHDQEQLIkeiqEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK08316  459 AVTAVVVPKAG-ATVTEDELI----AHCRARLAGFKVPKRVIFVDELPRNPSGKILKRELRE 515
4CL cd05904
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ...
72-615 2.46e-58

4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.


Pssm-ID: 341230 [Multi-domain]  Cd Length: 505  Bit Score: 204.01  E-value: 2.46e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  72 EKAGKKPSNPAFwwIN-RNGEEMrwSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPG 150
Cdd:cd05904    13 LFASAHPSRPAL--IDaATGRAL--TYAELERRVRRLAAGLA-KRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTA 87
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 151 TTQLTQKDILYRLQSSKANCIITndvlapaVDAVASKCENLHSKLIVSENsregwgnlkelmKVFLCHPGWSTVArswlT 230
Cdd:cd05904    88 NPLSTPAEIAKQVKDSGAKLAFT-------TAELAEKLASLALPVVLLDS------------AEFDSLSFSDLLF----E 144
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 231 ATSTSLVQAILSllsswdyrhasDSHTCvktkhneimAIFFTSGTSGYPKMTAHTHSSF-GLGLSVNGRFWLDLTPSDVM 309
Cdd:cd05904   145 ADEAEPPVVVIK-----------QDDVA---------ALLYSSGTTGRSKGVMLTHRNLiAMVAQFVAGEGSNSDSEDVF 204
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 310 WntsdtgwaksawssVFSPW--IQGACVFTHH----------LPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDIT- 376
Cdd:cd05904   205 L--------------CVLPMfhIYGLSSFALGllrlgatvvvMPRFDLEELLAAIERYKVTHLPVVPPIVLALVKSPIVd 270
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 377 SYKFKSLKHCVSAGEPITPDVTEKWRNK-TGLDIYEGYGQTET---VLICGNFKGMKIKPGSMGKPSPAFDVKIVDVN-G 451
Cdd:cd05904   271 KYDLSSLRQIMSGAAPLGKELIEAFRAKfPNVDLGQGYGMTEStgvVAMCFAPEKDRAKYGSVGRLVPNVEAKIVDPEtG 350
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 452 NVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGNFYI-TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEV 530
Cdd:cd05904   351 ESLPPNQTGELWI-----RGPSIMKGYLNNPEATAATIDKEGWLhTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAEL 425
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 531 ENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDykSHDQEQlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKT 610
Cdd:cd05904   426 EALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRKPG--SSLTED---EIMDFVAKQVAPYKKVRKVAFVDAIPKSPSGKI 500

                  ....*
gi 1370468924 611 KRNEL 615
Cdd:cd05904   501 LRKEL 505
PRK07514 PRK07514
malonyl-CoA synthase; Validated
94-618 7.59e-57

malonyl-CoA synthase; Validated


Pssm-ID: 181011 [Multi-domain]  Cd Length: 504  Bit Score: 199.72  E-value: 7.59e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  94 RWSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIIT 173
Cdd:PRK07514   28 RYTYGDLDAASARLANLLV-ALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAELDYFIGDAEPALVVC 106
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 174 NDVLAPAVDAVASKCenlhsklivsensreGWGNLKELMkvflchpgwstvarswlTATSTSLVQAIlsllsswdyRHAS 253
Cdd:PRK07514  107 DPANFAWLSKIAAAA---------------GAPHVETLD-----------------ADGTGSLLEAA---------AAAP 145
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 254 DSHTCVKTKHNEIMAIFFTSGTSGYPK--MTAHTH-SSFGLGLSVNGRFwldlTPSDVMwntsdtgwaksawssvfspwI 330
Cdd:PRK07514  146 DDFETVPRGADDLAAILYTSGTTGRSKgaMLSHGNlLSNALTLVDYWRF----TPDDVL--------------------I 201
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 331 QGACVFTHH------------------LPRFEPTSILQTLSKypITVFCSAPTVY-RMLVQNDITSYKFKSLKHCVSAGE 391
Cdd:PRK07514  202 HALPIFHTHglfvatnvallagasmifLPKFDPDAVLALMPR--ATVMMGVPTFYtRLLQEPRLTREAAAHMRLFISGSA 279
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 392 PITPDVTEKWRNKTGLDIYEGYGQTETVLICGN-FKGMKIkPGSMGKPSPAFDVKIVD-VNGNVLPPGQEGDIGIQVlPN 469
Cdd:PRK07514  280 PLLAETHREFQERTGHAILERYGMTETNMNTSNpYDGERR-AGTVGFPLPGVSLRVTDpETGAELPPGEIGMIEVKG-PN 357
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 470 rpfgLFTHYVDNPSKTASTLRGN-FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSS 548
Cdd:PRK07514  358 ----VFKGYWRMPEKTAEEFRADgFFITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIGV 433
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 549 PDPIRGEVVKAFVVLNPDyKSHDQEQLIKEIQEHVkkttAPYKYPRKVEFIQELPKTISGKTKRNELRKK 618
Cdd:PRK07514  434 PHPDFGEGVTAVVVPKPG-AALDEAAILAALKGRL----ARFKQPKRVFFVDELPRNTMGKVQKNLLREQ 498
PRK08276 PRK08276
long-chain-fatty-acid--CoA ligase; Validated
90-621 1.54e-55

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236215 [Multi-domain]  Cd Length: 502  Bit Score: 196.28  E-value: 1.54e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  90 GEEMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKAN 169
Cdd:PRK08276    7 PSGEVVTYGELEARSNRLAHGLRAL-GLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAK 85
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 170 CIITNDVLAPAVDAVASKCENlhsklivsensregwgNLKELMKVFLCHPGWstvaRSWLTATSTSLVQAILSLLSSWDy 249
Cdd:PRK08276   86 VLIVSAALADTAAELAAELPA----------------GVPLLLVVAGPVPGF----RSYEEALAAQPDTPIADETAGAD- 144
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 250 rhasdshtcvktkhneiMAifFTSGTSGYPK------MTAHTHSSFGLGLSVNGrFWLDLTPSDV------MWNTSDTGW 317
Cdd:PRK08276  145 -----------------ML--YSSGTTGRPKgikrplPGLDPDEAPGMMLALLG-FGMYGGPDSVylspapLYHTAPLRF 204
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 318 AKSAwssvfspwiqgacvftHHL-------PRFEPTSILQTLSKYPITVFCSAPTVY-RML-----VQNditSYKFKSLK 384
Cdd:PRK08276  205 GMSA----------------LALggtvvvmEKFDAEEALALIERYRVTHSQLVPTMFvRMLklpeeVRA---RYDVSSLR 265
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 385 HCVSAGEPITPDVTEK----WrnktGLDIYEGYGQTE----TVLICGNFKGmkiKPGSMGKPSPAfDVKIVDVNGNVLPP 456
Cdd:PRK08276  266 VAIHAAAPCPVEVKRAmidwW----GPIIHEYYASSEgggvTVITSEDWLA---HPGSVGKAVLG-EVRILDEDGNELPP 337
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 457 GQEGDIGIQvLPNRPFglftHYVDNPSKTASTLRGNFYIT-GDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALN 535
Cdd:PRK08276  338 GEIGTVYFE-MDGYPF----EYHNDPEKTAAARNPHGWVTvGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLV 412
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 536 EHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:PRK08276  413 THPKVADVAVFGVPDEEMGERVKAVVQPADGAD--AGDALAAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRL 490

                  ....*.
gi 1370468924 616 RKKEWK 621
Cdd:PRK08276  491 RDRYWE 496
prpE PRK10524
propionyl-CoA synthetase; Provisional
76-612 1.02e-54

propionyl-CoA synthetase; Provisional


Pssm-ID: 182517 [Multi-domain]  Cd Length: 629  Bit Score: 196.71  E-value: 1.02e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  76 KKPSNPAFWWIN-RNGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTG---TVLIPG- 150
Cdd:PRK10524   65 KRPEQLALIAVStETDEERTYTFRQLHDEVNRMAAML-RSLGVQRGDRVLIYMPMIAEAAFAMLACARIGaihSVVFGGf 143
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 151 -TTQLTQkdilyRLQSSKANCIITNDVLAPAVDAVASKCEnLHSKLIVSENSREgwgnlkelmKVFLCHPGWSTVARswl 229
Cdd:PRK10524  144 aSHSLAA-----RIDDAKPVLIVSADAGSRGGKVVPYKPL-LDEAIALAQHKPR---------HVLLVDRGLAPMAR--- 205
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 230 tatstslVQAILSLLSSWDYRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSDVM 309
Cdd:PRK10524  206 -------VAGRDVDYATLRAQHLGARVPVEWLESNEPSYILYTSGTTGKPKGVQRDTGGYAVALATSMDTIFGGKAGETF 278
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 310 WNTSDTGWAKSAWSSVFSPWIQG-ACVFTHHLP-RFEPTSILQTLSKYPITVFCSAPTVYRMLVQND---ITSYKFKSLK 384
Cdd:PRK10524  279 FCASDIGWVVGHSYIVYAPLLAGmATIMYEGLPtRPDAGIWWRIVEKYKVNRMFSAPTAIRVLKKQDpalLRKHDLSSLR 358
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 385 HCVSAGEPITpDVTEKWRNKT-GLDIYEGYGQTET----VLICGNFKGMKIKPGSMGKPSPAFDVKIVD-VNGNVLPPGQ 458
Cdd:PRK10524  359 ALFLAGEPLD-EPTASWISEAlGVPVIDNYWQTETgwpiLAIARGVEDRPTRLGSPGVPMYGYNVKLLNeVTGEPCGPNE 437
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 459 EGDIGIQV-LPnrPFGLFTHYVDNP---SKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENAL 534
Cdd:PRK10524  438 KGVLVIEGpLP--PGCMQTVWGDDDrfvKTYWSLFGRQVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESI 515
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 535 NEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQEQ---LIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTK 611
Cdd:PRK10524  516 SSHPAVAEVAVVGVKDALKGQVAVAFVVPKDSDSLADREArlaLEKEIMALVDSQLGAVARPARVWFVSALPKTRSGKLL 595

                  .
gi 1370468924 612 R 612
Cdd:PRK10524  596 R 596
PRK08974 PRK08974
long-chain-fatty-acid--CoA ligase FadD;
72-619 1.17e-54

long-chain-fatty-acid--CoA ligase FadD;


Pssm-ID: 236359 [Multi-domain]  Cd Length: 560  Bit Score: 195.27  E-value: 1.17e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  72 EKAGKK-PSNPAFwwINRnGEEMrwSFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPG 150
Cdd:PRK08974   30 EQAVARyADQPAF--INM-GEVM--TFRKLEERSRAFAAYLQNGLGLKKGDRVALMMPNLLQYPIALFGILRAGMIVVNV 104
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 151 TTQLTQKDILYRLQSSKANCIITndvlapaVDAVASKCENlhskliVSENSregwgnlkELMKVFLCHPGwstvarSWLT 230
Cdd:PRK08974  105 NPLYTPRELEHQLNDSGAKAIVI-------VSNFAHTLEK------VVFKT--------PVKHVILTRMG------DQLS 157
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 231 ATSTSLV----QAILSLLSSWDYRHASdSHTCVKTK-----------HNEIMAIF-FTSGTSGYPKMTAHTHSsfglgls 294
Cdd:PRK08974  158 TAKGTLVnfvvKYIKRLVPKYHLPDAI-SFRSALHKgrrmqyvkpelVPEDLAFLqYTGGTTGVAKGAMLTHR------- 229
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 295 vngrfwldltpsDVMWNTSDTGWAKSAWSSVFSPWIQGA------------CVFTHHL---------PRFEPTSIlQTLS 353
Cdd:PRK08974  230 ------------NMLANLEQAKAAYGPLLHPGKELVVTAlplyhifaltvnCLLFIELggqnllitnPRDIPGFV-KELK 296
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 354 KYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTE-TVLICGNFKGMKIK 431
Cdd:PRK08974  297 KYPFTAITGVNTLFNALLNNeEFQELDFSSLKLSVGGGMAVQQAVAERWVKLTGQYLLEGYGLTEcSPLVSVNPYDLDYY 376
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 432 PGSMGKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQVLPNrpfglfthYVDNPSKTASTLRGNFYITGDRGYMDKDGYF 508
Cdd:PRK08974  377 SGSIGLPVPSTEIKLVDDDGNEVPPGEPGELwvkGPQVMLG--------YWQRPEATDEVIKDGWLATGDIAVMDEEGFL 448
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 509 WFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPdyKSHDQEQLIKEIQEHVkkttA 588
Cdd:PRK08974  449 RIVDRKKDMILVSGFNVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFVVKKD--PSLTEEELITHCRRHL----T 522
                         570       580       590
                  ....*....|....*....|....*....|.
gi 1370468924 589 PYKYPRKVEFIQELPKTISGKTKRNELRKKE 619
Cdd:PRK08974  523 GYKVPKLVEFRDELPKSNVGKILRRELRDEA 553
PRK05605 PRK05605
long-chain-fatty-acid--CoA ligase; Validated
63-617 1.33e-54

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235531 [Multi-domain]  Cd Length: 573  Bit Score: 195.22  E-value: 1.33e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  63 DVLDqwtdkEKAGKKPSNPAFWWInrnGEEMrwSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLR 142
Cdd:PRK05605   36 DLYD-----NAVARFGDRPALDFF---GATT--TYAELGKQVRRAAAGL-RALGVRPGDRVAIVLPNCPQHIVAFYAVLR 104
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 143 TGTVLIPGTTQLTQKDILYRLQSSKANCIItndvlapAVDAVASKCENLHSKL----IVSENSREGWGNLKELmKVFLCH 218
Cdd:PRK05605  105 LGAVVVEHNPLYTAHELEHPFEDHGARVAI-------VWDKVAPTVERLRRTTpletIVSVNMIAAMPLLQRL-ALRLPI 176
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 219 PGWSTvARSWLTATSTSLVqailsllsSWDYRHAS------DSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSsfglG 292
Cdd:PRK05605  177 PALRK-ARAALTGPAPGTV--------PWETLVDAaiggdgSDVSHPRPTPDDVALILYTSGTTGKPKGAQLTHR----N 243
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 293 LSVN---GRFWLDLTPSD----------------VMWNTsdtgwaksawssvFSPWIQGACVFthhLPRFEPTSILQTLS 353
Cdd:PRK05605  244 LFANaaqGKAWVPGLGDGpervlaalpmfhayglTLCLT-------------LAVSIGGELVL---LPAPDIDLILDAMK 307
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 354 KYPITVFCSAPTVYRMLVQN------DITSykfksLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETV-LICGNFK 426
Cdd:PRK05605  308 KHPPTWLPGVPPLYEKIAEAaeergvDLSG-----VRNAFSGAMALPVSTVELWEKLTGGLLVEGYGLTETSpIIVGNPM 382
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 427 GMKIKPGSMGKPSPAFDVKIVDVN--GNVLPPGQEGDI---GIQVlpnrpfglFTHYVDNPSKTASTLRGNFYITGDRGY 501
Cdd:PRK05605  383 SDDRRPGYVGVPFPDTEVRIVDPEdpDETMPDGEEGELlvrGPQV--------FKGYWNRPEETAKSFLDGWFRTGDVVV 454
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 502 MDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyKSHDQEQLikeiQE 581
Cdd:PRK05605  455 MEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPG-AALDPEGL----RA 529
                         570       580       590
                  ....*....|....*....|....*....|....*.
gi 1370468924 582 HVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK05605  530 YCREHLTRYKVPRRFYHVDELPRDQLGKVRRREVRE 565
OSB_CoA_lg cd05912
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ...
260-617 1.55e-54

O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.


Pssm-ID: 341238 [Multi-domain]  Cd Length: 411  Bit Score: 191.02  E-value: 1.55e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 260 KTKHNEIMAIFFTSGTSGYPK---MTAHTH------SSFGLGLSVNGRfWLDLTPsdvMWNTSdtgwaksAWSSVFSPWI 330
Cdd:cd05912    73 DVKLDDIATIMYTSGTTGKPKgvqQTFGNHwwsaigSALNLGLTEDDN-WLCALP---LFHIS-------GLSILMRSVI 141
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 331 QGACVFTHhlPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSYKfKSLKHCVSAGEPITPDVTEKWRNKtGLDIY 410
Cdd:cd05912   142 YGMTVYLV--DKFDAEQVLHLINSGKVTIISVVPTMLQRLLEILGEGYP-NNLRCILLGGGPAPKPLLEQCKEK-GIPVY 217
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 411 EGYGQTETV--LICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGnvlPPGQEGDIGIQVlPNRPFGlfthYVDNPSKTAST 488
Cdd:cd05912   218 QSYGMTETCsqIVTLSPEDALNKIGSAGKPLFPVELKIEDDGQ---PPYEVGEILLKG-PNVTKG----YLNRPDATEES 289
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 489 LRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYk 568
Cdd:cd05912   290 FENGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSERPI- 368
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*....
gi 1370468924 569 shDQEQLIKEIQEHVKKttapYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05912   369 --SEEELIAYCSEKLAK----YKVPKKIYFVDELPRTASGKLLRHELKQ 411
PRK06178 PRK06178
acyl-CoA synthetase; Validated
63-615 3.12e-53

acyl-CoA synthetase; Validated


Pssm-ID: 235724 [Multi-domain]  Cd Length: 567  Bit Score: 191.41  E-value: 3.12e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  63 DVLDQWtdkekAGKKPSNPAFWWINRngeemRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLR 142
Cdd:PRK06178   37 EYLRAW-----ARERPQRPAIIFYGH-----VITYAELDELSDRFAALL-RQRGVGAGDRVAVFLPNCPQFHIVFFGILK 105
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 143 TGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCEnlhsklivsensregwgnLKELMKVFLCH--PG 220
Cdd:PRK06178  106 LGAVHVPVSPLFREHELSYELNDAGAEVLLALDQLAPVVEQVRAETS------------------LRHVIVTSLADvlPA 167
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 221 WSTVARSWLTATSTSLVQAILSLLSSwdYRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFW 300
Cdd:PRK06178  168 EPTLPLPDSLRAPRLAAAGAIDLLPA--LRACTAPVPLPPPALDALAALNYTGGTTGMPKGCEHTQRDMVYTAAAAYAVA 245
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 301 LDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFThhLPRFEPTSILQTLSKYPITVfcsapTVyrMLVQN------- 373
Cdd:PRK06178  246 VVGGEDSVFLSFLPEFWIAGENFGLLFPLFSGATLVL--LARWDAVAFMAAVERYRVTR-----TV--MLVDNavelmdh 316
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 374 -DITSYKFKSLKH--CVSAGEPITPDVTEKWRNKTGLDIYEG-YGQTETvLICGNF-KGM-------KIKPGSMGKPSPA 441
Cdd:PRK06178  317 pRFAEYDLSSLRQvrVVSFVKKLNPDYRQRWRALTGSVLAEAaWGMTET-HTCDTFtAGFqdddfdlLSQPVFVGLPVPG 395
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 442 FDVKIVD-VNGNVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILS 520
Cdd:PRK06178  396 TEFKICDfETGELLPLGAEGEIVV-----RTPSLLKGYWNKPEATAEALRDGWLHTGDIGKIDEQGFLHYLGRRKEMLKV 470
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 521 SGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyksHDQEQliKEIQEHVKKTTAPYKYPrKVEFIQ 600
Cdd:PRK06178  471 NGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPG---ADLTA--AALQAWCRENMAVYKVP-EIRIVD 544
                         570
                  ....*....|....*
gi 1370468924 601 ELPKTISGKTKRNEL 615
Cdd:PRK06178  545 ALPMTATGKVRKQDL 559
ACLS-CaiC cd17637
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ...
340-612 5.18e-53

acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341292 [Multi-domain]  Cd Length: 333  Bit Score: 184.78  E-value: 5.18e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 340 LPRFEPTSILQTLSKYPITVFCS-APTVYRMLVQNDITSYKFKSLKHcVSAGEpiTPDVTEKWRNKTGLDIYEGYGQTET 418
Cdd:cd17637    72 MEKFDPAEALELIEEEKVTLMGSfPPILSNLLDAAEKSGVDLSSLRH-VLGLD--APETIQRFEETTGATFWSLYGQTET 148
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 419 ---VLICGNFKgmkiKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGNFYI 495
Cdd:cd17637   149 sglVTLSPYRE----RPGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVV-----RGPLVFQGYWNLPELTAYTFRNGWHH 219
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 496 TGDRGYMDKDGYFWFVAR--ADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyKSHDQE 573
Cdd:cd17637   220 TGDLGRFDEDGYLWYAGRkpEKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCVLKPG-ATLTAD 298
                         250       260       270
                  ....*....|....*....|....*....|....*....
gi 1370468924 574 QLIkeiqEHVKKTTAPYKYPRKVEFIQELPKTISGKTKR 612
Cdd:cd17637   299 ELI----EFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
PRK03640 PRK03640
o-succinylbenzoate--CoA ligase;
91-619 9.96e-52

o-succinylbenzoate--CoA ligase;


Pssm-ID: 235146 [Multi-domain]  Cd Length: 483  Bit Score: 185.55  E-value: 9.96e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  91 EEMRWSFEELGSLSRKFANILSEACsLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANC 170
Cdd:PRK03640   24 EEKKVTFMELHEAVVSVAGKLAALG-VKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREELLWQLDDAEVKC 102
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 171 IITNDVLAPAVDAVASkcenlhskLIVSEnsregwgnlkeLMKVFLCHPgwstvarswltatstslvqailSLLSSWDYr 250
Cdd:PRK03640  103 LITDDDFEAKLIPGIS--------VKFAE-----------LMNGPKEEA----------------------EIQEEFDL- 140
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 251 hasdshtcvktkhNEIMAIFFTSGTSGYPK---MTAHTH------SSFGLGLSVNGRfWLDLTPsdvMWNTSdtgwaksA 321
Cdd:PRK03640  141 -------------DEVATIMYTSGTTGKPKgviQTYGNHwwsavgSALNLGLTEDDC-WLAAVP---IFHIS-------G 196
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 322 WSSVFSPWIQGACVFTHhlPRFEPTSILQTLSKYPITVFCSAPT-VYRMLVQNDITSYKfKSLKHCVSAGEPITPDVTEK 400
Cdd:PRK03640  197 LSILMRSVIYGMRVVLV--EKFDAEKINKLLQTGGVTIISVVSTmLQRLLERLGEGTYP-SSFRCMLLGGGPAPKPLLEQ 273
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 401 WRNKtGLDIYEGYGQTETV-LICG-NFKGMKIKPGSMGKPSPAFDVKIVDvNGNVLPPGQEGDIGIQVlPNrpfgLFTHY 478
Cdd:PRK03640  274 CKEK-GIPVYQSYGMTETAsQIVTlSPEDALTKLGSAGKPLFPCELKIEK-DGVVVPPFEEGEIVVKG-PN----VTKGY 346
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 479 VDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVK 558
Cdd:PRK03640  347 LNREDATRETFQDGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPV 426
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1370468924 559 AFVVLNpdyKSHDQEQLIKEIQEHVkkttAPYKYPRKVEFIQELPKTISGKTKRNELRKKE 619
Cdd:PRK03640  427 AFVVKS---GEVTEEELRHFCEEKL----AKYKVPKRFYFVEELPRNASGKLLRHELKQLV 480
PRK06839 PRK06839
o-succinylbenzoate--CoA ligase;
90-615 1.82e-51

o-succinylbenzoate--CoA ligase;


Pssm-ID: 168698 [Multi-domain]  Cd Length: 496  Bit Score: 185.06  E-value: 1.82e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  90 GEEMRWSFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKAN 169
Cdd:PRK06839   23 TEEEEMTYKQLHEYVSKVAAYLIYELNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENELIFQLKDSGTT 102
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 170 CIITNDVLApavdavaskcenlhsklivsensregwgNLKELMKvflchpGWSTVARS-WLTATSTSLVQAIlsllSSWD 248
Cdd:PRK06839  103 VLFVEKTFQ----------------------------NMALSMQ------KVSYVQRViSITSLKEIEDRKI----DNFV 144
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 249 YRHASDShtcvktkhneiMAIFFTSGTSGYPKMTAHTHSSFGLGlSVNGRFWLDLTPSDV------MWNTSDTGWAksaw 322
Cdd:PRK06839  145 EKNESAS-----------FIICYTSGTTGKPKGAVLTQENMFWN-ALNNTFAIDLTMHDRsivllpLFHIGGIGLF---- 208
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 323 ssVFSPWIQGACVFTHHlpRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEPITPDVTEKW 401
Cdd:PRK06839  209 --AFPTLFAGGVIIVPR--KFEPTKALSMIEKHKVTVVMGVPTIHQALINCsKFETTNLQSVRWFYNGGAPCPEELMREF 284
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 402 RNKtGLDIYEGYGQTET-----VLICGNFKGmkiKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVlPNrpfgLFT 476
Cdd:PRK06839  285 IDR-GFLFGQGFGMTETsptvfMLSEEDARR---KVGSIGKPVLFCDYELIDENKNKVEVGEVGELLIRG-PN----VMK 355
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 477 HYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEV 556
Cdd:PRK06839  356 EYWNRPDATEETIQDGWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEI 435
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1370468924 557 VKAFVVLNPDYKSHDqeqliKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:PRK06839  436 PIAFIVKKSSSVLIE-----KDVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQL 489
PRK06155 PRK06155
crotonobetaine/carnitine-CoA ligase; Provisional
92-616 3.32e-51

crotonobetaine/carnitine-CoA ligase; Provisional


Pssm-ID: 235719 [Multi-domain]  Cd Length: 542  Bit Score: 185.35  E-value: 3.32e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  92 EMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCI 171
Cdd:PRK06155   44 GTRWTYAEAARAAAAAAHALAAA-GVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALRGPQLEHILRNSGARLL 122
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 172 ITNDVLAPAVDAVASKCENLHSKLIVSENsregwgnlkelmkvflchPGWStVARSWLTATSTSLVQAIlsllsswdyrh 251
Cdd:PRK06155  123 VVEAALLAALEAADPGDLPLPAVWLLDAP------------------ASVS-VPAGWSTAPLPPLDAPA----------- 172
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 252 asdshTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFglglsvngrFW--------LDLTPSDVMWNTSDTgWAKSAWS 323
Cdd:PRK06155  173 -----PAAAVQPGDTAAILYTSGTTGPSKGVCCPHAQF---------YWwgrnsaedLEIGADDVLYTTLPL-FHTNALN 237
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 324 SVFSPWIQGAcvfTHHL-PRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDIT-SYKFKSLKHCVSAGEPitPDVTEKW 401
Cdd:PRK06155  238 AFFQALLAGA---TYVLePRFSASGFWPAVRRHGATVTYLLGAMVSILLSQPAReSDRAHRVRVALGPGVP--AALHAAF 312
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 402 RNKTGLDIYEGYGQTETVLICGNFKGMKiKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVlpNRPFGLFTHYVDN 481
Cdd:PRK06155  313 RERFGVDLLDGYGSTETNFVIAVTHGSQ-RPGSMGRLAPGFEARVVDEHDQELPDGEPGELLLRA--DEPFAFATGYFGM 389
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 482 PSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFV 561
Cdd:PRK06155  390 PEKTVEAWRNLWFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEDEVMAAV 469
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1370468924 562 VLNPDyKSHDQEQLIkeiqEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PRK06155  470 VLRDG-TALEPVALV----RHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLR 519
PRK07059 PRK07059
Long-chain-fatty-acid--CoA ligase; Validated
96-616 1.71e-50

Long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235923 [Multi-domain]  Cd Length: 557  Bit Score: 183.68  E-value: 1.71e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITND 175
Cdd:PRK07059   50 TYGELDELSRALAAWL-QSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIVVLE 128
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 VLAPAVDAVASKCENLHsklIVSENSREGWGnLKELMKVFLCH------PGWSTV-ARSWLTATSTSlvqailsllsswd 248
Cdd:PRK07059  129 NFATTVQQVLAKTAVKH---VVVASMGDLLG-FKGHIVNFVVRrvkkmvPAWSLPgHVRFNDALAEG------------- 191
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 249 yrhASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFglglsvngrfwldltPSDVMWNTSdtgWAKSAWSSvfsP 328
Cdd:PRK07059  192 ---ARQTFKPVKLGPDDVAFLQYTGGTTGVSKGATLLHRNI---------------VANVLQMEA---WLQPAFEK---K 247
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 329 WIQGACVFTHHLP---------------RFEPTSIL-----------QTLSKYPITVFCSAPTVYRMLVQN-DITSYKFK 381
Cdd:PRK07059  248 PRPDQLNFVCALPlyhifaltvcgllgmRTGGRNILipnprdipgfiKELKKYQVHIFPAVNTLYNALLNNpDFDKLDFS 327
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 382 SLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTET--VLICGNFKGMKIKpGSMGKPSPAFDVKIVDVNGNVLPPGQE 459
Cdd:PRK07059  328 KLIVANGGGMAVQRPVAERWLEMTGCPITEGYGLSETspVATCNPVDATEFS-GTIGLPLPSTEVSIRDDDGNDLPLGEP 406
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 460 GDI---GIQVLPNrpfglfthYVDNPSKTASTLRGN-FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALN 535
Cdd:PRK07059  407 GEIcirGPQVMAG--------YWNRPDETAKVMTADgFFRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVA 478
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 536 EHPSVAESAVVSSPDPIRGEVVKAFVVlnpdykSHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:PRK07059  479 SHPGVLEVAAVGVPDEHSGEAVKLFVV------KKDPALTEEDVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRREL 552

                  .
gi 1370468924 616 R 616
Cdd:PRK07059  553 R 553
PRK06087 PRK06087
medium-chain fatty-acid--CoA ligase;
94-617 1.73e-50

medium-chain fatty-acid--CoA ligase;


Pssm-ID: 180393 [Multi-domain]  Cd Length: 547  Bit Score: 183.41  E-value: 1.73e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  94 RWSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIIt 173
Cdd:PRK06087   49 SYTYSALDHAASRLANWLL-AKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWREAELVWVLNKCQAKMFF- 126
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 174 ndvlAPAVDAvaskcENLHSKLIVSenSREGWGNLKELMKVFLCHPGWSTVARSWLTATSTSLVQAIlsllsswdyrhas 253
Cdd:PRK06087  127 ----APTLFK-----QTRPVDLILP--LQNQLPQLQQIVGVDKLAPATSSLSLSQIIADYEPLTTAI------------- 182
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 254 dshtcvKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLG-LSVNGRfwLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQG 332
Cdd:PRK06087  183 ------TTHGDELAAVLFTSGTEGLPKGVMLTHNNILASeRAYCAR--LNLTWQDVFMMPAPLGHATGFLHGVTAPFLIG 254
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 333 ACVFThhLPRFEPTSILQTLSKYPITVFCSA-PTVYRMLVQNDITSYKFKSLKHCVSAGEPITPDVTEK-WRNktGLDIY 410
Cdd:PRK06087  255 ARSVL--LDIFTPDACLALLEQQRCTCMLGAtPFIYDLLNLLEKQPADLSALRFFLCGGTTIPKKVAREcQQR--GIKLL 330
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 411 EGYGQTETV--LICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDiGIQVLPNrpfgLFTHYVDNPSKTAST 488
Cdd:PRK06087  331 SVYGSTESSphAVVNLDDPLSRFMHTDGYAAAGVEIKVVDEARKTLPPGCEGE-EASRGPN----VFMGYLDEPELTARA 405
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 489 L--RGNFYiTGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPD 566
Cdd:PRK06087  406 LdeEGWYY-SGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAP 484
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1370468924 567 YKSHDQEQLIKEIQEhvkKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK06087  485 HHSLTLEEVVAFFSR---KRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRK 532
PRK06188 PRK06188
acyl-CoA synthetase; Validated
78-620 3.13e-50

acyl-CoA synthetase; Validated


Pssm-ID: 235731 [Multi-domain]  Cd Length: 524  Bit Score: 182.11  E-value: 3.13e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  78 PSNPAFWWINRngeemRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTG---TVLIP-GTTQ 153
Cdd:PRK06188   26 PDRPALVLGDT-----RLTYGQLADRISRYIQAF-EALGLGTGDAVALLSLNRPEVLMAIGAAQLAGlrrTALHPlGSLD 99
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 154 ltqkDILYRLQSSKANCIITNDvlAPAVD---AVASKCENLhsklivsensregwgnlkelmkvflchpgwstvaRSWLT 230
Cdd:PRK06188  100 ----DHAYVLEDAGISTLIVDP--APFVEralALLARVPSL----------------------------------KHVLT 139
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 231 ATSTSLVQAILSLLSSWDYRHASDSHTcvktkHNEIMAIFFTSGTSGYPKMTAHTHSSFG---------LGLSVNGRFwL 301
Cdd:PRK06188  140 LGPVPDGVDLLAAAAKFGPAPLVAAAL-----PPDIAGLAYTGGTTGKPKGVMGTHRSIAtmaqiqlaeWEWPADPRF-L 213
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 302 DLTPsdvmwnTSDTGWAKsawssvFSPWIQ-GACVftHHLPRFEPTSILQTLSKYPITVFCSAPT-VYRMLVQNDITSYK 379
Cdd:PRK06188  214 MCTP------LSHAGGAF------FLPTLLrGGTV--IVLAKFDPAEVLRAIEEQRITATFLVPTmIYALLDHPDLRTRD 279
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 380 FKSLKHCVSAGEPITPDvtekwRNKTGLDIY-----EGYGQTE-----TVLICGNFKGMKIKP-GSMGKPSPAFDVKIVD 448
Cdd:PRK06188  280 LSSLETVYYGASPMSPV-----RLAEAIERFgpifaQYYGQTEapmviTYLRKRDHDPDDPKRlTSCGRPTPGLRVALLD 354
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 449 VNGNVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPF 528
Cdd:PRK06188  355 EDGREVAQGEVGEICV-----RGPLVMDGYWNRPEETAEAFRDGWLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPR 429
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 529 EVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyKSHDQEqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISG 608
Cdd:PRK06188  430 EVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPG-AAVDAA----ELQAHVKERKGSVHAPKQVDFVDSLPLTALG 504
                         570
                  ....*....|..
gi 1370468924 609 KTKRNELRKKEW 620
Cdd:PRK06188  505 KPDKKALRARYW 516
A_NRPS cd05930
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ...
253-615 1.23e-49

The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341253 [Multi-domain]  Cd Length: 444  Bit Score: 178.49  E-value: 1.23e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 253 SDSH-TCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFglglsVNGRFW----LDLTPSDVMWNTSDTGWAKSAWSsVFS 327
Cdd:cd05930    81 EDSGaKLVLTDPDDLAYVIYTSGSTGKPKGVMVEHRGL-----VNLLLWmqeaYPLTPGDRVLQFTSFSFDVSVWE-IFG 154
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 328 PWIQGACVftHHLP---RFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSyKFKSLKHCVSAGEPITPDVTEKWR-N 403
Cdd:cd05930   155 ALLAGATL--VVLPeevRKDPEALADLLAEEGITVLHLTPSLLRLLLQELELA-ALPSLRLVLVGGEALPPDLVRRWReL 231
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 404 KTGLDIYEGYGQTETVLICGNF--KGMKIKPGSM--GKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQVlpNRpfGlft 476
Cdd:cd05930   232 LPGARLVNLYGPTEATVDATYYrvPPDDEEDGRVpiGRPIPNTRVYVLDENLRPVPPGVPGELyigGAGL--AR--G--- 304
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 477 hYVDNPSKTASTLRGN-------FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSP 549
Cdd:cd05930   305 -YLNRPELTAERFVPNpfgpgerMYRTGDLVRWLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLAHPGVREAAVVARE 383
                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1370468924 550 DPIRGEVVKAFVVLNPdykshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd05930   384 DGDGEKRLVAYVVPDE-----GGELDEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
PRK07529 PRK07529
AMP-binding domain protein; Validated
74-619 2.57e-49

AMP-binding domain protein; Validated


Pssm-ID: 236043 [Multi-domain]  Cd Length: 632  Bit Score: 181.69  E-value: 2.57e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  74 AGKKPSNPAFWWI---NRNGEEMRWSFEELgsLSR--KFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVlI 148
Cdd:PRK07529   35 AARHPDAPALSFLldaDPLDRPETWTYAEL--LADvtRTANLLH-SLGVGPGDVVAFLLPNLPETHFALWGGEAAGIA-N 110
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 149 PGTTQLTQKDILYRLQSSKANCIITndvLAPAVDA-VASKCENLHSKLivsensregwGNLKELMKVFL--CHPGWSTVA 225
Cdd:PRK07529  111 PINPLLEPEQIAELLRAAGAKVLVT---LGPFPGTdIWQKVAEVLAAL----------PELRTVVEVDLarYLPGPKRLA 177
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 226 RSWLTATSTSLVQAILSLLSswdyRHASDSHTCVKTKHNEIMAIFF-TSGTSGYPKMTAHTHSsfglGLSVNGrfW---- 300
Cdd:PRK07529  178 VPLIRRKAHARILDFDAELA----RQPGDRLFSGRPIGPDDVAAYFhTGGTTGMPKLAQHTHG----NEVANA--Wlgal 247
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 301 -LDLTPSDVMW--------NTSDTGwaksawssVFSPWIQGACVFThhlprfePTS-----------ILQTLSKYPITVF 360
Cdd:PRK07529  248 lLGLGPGDTVFcglplfhvNALLVT--------GLAPLARGAHVVL-------ATPqgyrgpgvianFWKIVERYRINFL 312
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 361 CSAPTVYRMLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTE-TVLICGNFKGMKIKPGSMGKPS 439
Cdd:PRK07529  313 SGVPTVYAALLQVPVDGHDISSLRYALCGAAPLPVEVFRRFEAATGVRIVEGYGLTEaTCVSSVNPPDGERRIGSVGLRL 392
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 440 PAFDVKIV--DVNGNVLPPGQEGDIGIQVLPNrPfGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDV 517
Cdd:PRK07529  393 PYQRVRVVilDDAGRYLRDCAVDEVGVLCIAG-P-NVFSGYLEAAHNKGLWLEDGWLNTGDLGRIDADGYFWLTGRAKDL 470
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 518 ILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyKSHDQEQLIKEIQEHVKKTTApykYPRKVE 597
Cdd:PRK07529  471 IIRGGHNIDPAAIEEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPG-ASATEAELLAFARDHIAERAA---VPKHVR 546
                         570       580
                  ....*....|....*....|..
gi 1370468924 598 FIQELPKTISGKTKRNELRKKE 619
Cdd:PRK07529  547 ILDALPKTAVGKIFKPALRRDA 568
CHC_CoA_lg cd05903
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ...
94-617 9.01e-49

Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.


Pssm-ID: 341229 [Multi-domain]  Cd Length: 437  Bit Score: 176.03  E-value: 9.01e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  94 RWSFEELGSLSRKFANILSEAcSLQRGDRVILILPrvpEWWLANV---ACLRTGTVLIPGTTQLTQKDILYRLQSSKAnc 170
Cdd:cd05903     1 RLTYSELDTRADRLAAGLAAL-GVGPGDVVAFQLP---NWWEFAVlylACLRIGAVTNPILPFFREHELAFILRRAKA-- 74
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 171 iitndvlapavdavaskcenlhsklivsensregwgnlkelmKVFLchpgwstVARSWLTatstslvqailsllsswdYR 250
Cdd:cd05903    75 ------------------------------------------KVFV-------VPERFRQ------------------FD 87
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 251 HASDShtcvktkhNEIMAIFFTSGTSGYPKMTAHTHSSfglgLSVNGRFW---LDLTPSDVMWNTSDTGWAKSAWSSVFS 327
Cdd:cd05903    88 PAAMP--------DAVALLLFTSGTTGEPKGVMHSHNT----LSASIRQYaerLGLGPGDVFLVASPMAHQTGFVYGFTL 155
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 328 PWIQGACVftHHLPRFEPTSILQTLSKYPITVFCSAPT-VYRMLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTG 406
Cdd:cd05903   156 PLLLGAPV--VLQDIWDPDKALALMREHGVTFMMGATPfLTDLLNAVEEAGEPLSRLRTFVCGGATVPRSLARRAAELLG 233
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 407 LDIYEGYGQTETVLICGNfkgmkIKPG-------SMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIqvlpnRPFGLFTHYV 479
Cdd:cd05903   234 AKVCSAYGSTECPGAVTS-----ITPApedrrlyTDGRPLPGVEIKVVDDTGATLAPGVEGELLS-----RGPSVFLGYL 303
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 480 DNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKA 559
Cdd:cd05903   304 DRPDLTADAAPEGWFRTGDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACA 383
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1370468924 560 FVVLNPdykshDQEQLIKEIQEHV-KKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05903   384 VVVTKS-----GALLTFDELVAYLdRQGVAKQYWPERLVHVDDLPRTPSGKVQKFRLRE 437
PRK07470 PRK07470
acyl-CoA synthetase; Validated
74-618 1.26e-48

acyl-CoA synthetase; Validated


Pssm-ID: 180988 [Multi-domain]  Cd Length: 528  Bit Score: 177.93  E-value: 1.26e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  74 AGKKPSNPAFWWinrnGEEmRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQ 153
Cdd:PRK07470   17 ARRFPDRIALVW----GDR-SWTWREIDARVDALAAAL-AARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNFR 90
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 154 LTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIVSEnsregwgnlkelmkvflchpgwstvarswltATS 233
Cdd:PRK07470   91 QTPDEVAYLAEASGARAMICHADFPEHAAAVRAASPDLTHVVAIGG-------------------------------ARA 139
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 234 TSLVQAILSllsswdyRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSfgLGLSVNGRFwLDLTPsdvmwnts 313
Cdd:PRK07470  140 GLDYEALVA-------RHLGARVANAAVDHDDPCWFFFTSGTTGRPKAAVLTHGQ--MAFVITNHL-ADLMP-------- 201
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 314 dtGWAKSAWSSVFSPWIQGACVftHHL--------------PRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSY 378
Cdd:PRK07470  202 --GTTEQDASLVVAPLSHGAGI--HQLcqvargaatvllpsERFDPAEVWALVERHRVTNLFTVPTILKMLVEHpAVDRY 277
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 379 KFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTE-----TVL-ICGNF--KGMKIKPGSMGKPSPAFDVKIVDVN 450
Cdd:PRK07470  278 DHSSLRYVIYAGAPMYRADQKRALAKLGKVLVQYFGLGEvtgniTVLpPALHDaeDGPDARIGTCGFERTGMEVQIQDDE 357
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 451 GNVLPPGQEGDI---GIQVlpnrpfglFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGP 527
Cdd:PRK07470  358 GRELPPGETGEIcviGPAV--------FAGYYNNPEANAKAFRDGWFRTGDLGHLDARGFLYITGRASDMYISGGSNVYP 429
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 528 FEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNpDYKSHDQEqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTIS 607
Cdd:PRK07470  430 REIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVAR-DGAPVDEA----ELLAWLDGKVARYKLPKRFFFWDALPKSGY 504
                         570
                  ....*....|.
gi 1370468924 608 GKTKRNELRKK 618
Cdd:PRK07470  505 GKITKKMVREE 515
PRK07786 PRK07786
long-chain-fatty-acid--CoA ligase; Validated
77-618 1.37e-48

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 169098 [Multi-domain]  Cd Length: 542  Bit Score: 178.05  E-value: 1.37e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  77 KPSNPAFWWInrnGEEMRWSfeELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQ 156
Cdd:PRK07786   30 QPDAPALRFL---GNTTTWR--ELDDRVAALAGALSRR-GVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTP 103
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 157 KDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIVSENSREGWGNLKELMkvflchpgwstvarswltatstsl 236
Cdd:PRK07786  104 PEIAFLVSDCGAHVVVTEAALAPVATAVRDIVPLLSTVVVAGGSSDDSVLGYEDLL------------------------ 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 237 vqailsllsswdyRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGlGLSVNG-RFWLDLTPSDVMWNTSDT 315
Cdd:PRK07786  160 -------------AEAGPAHAPVDIPNDSPALIMYTSGTTGRPKGAVLTHANLT-GQAMTClRTNGADINSDVGFVGVPL 225
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 316 gWAKSAWSSVFSPWIQGACVFTHHLPRFEPTSILQTLSKYPIT-VFCsAPTVYRMLVQNDITSYKFKSLKhCVSAGEPIT 394
Cdd:PRK07786  226 -FHIAGIGSMLPGLLLGAPTVIYPLGAFDPGQLLDVLEAEKVTgIFL-VPAQWQAVCAEQQARPRDLALR-VLSWGAAPA 302
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 395 PDVTEKWRNKT--GLDIYEGYGQTE----TVLICGNFKGMKIkpGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIgiqvlP 468
Cdd:PRK07786  303 SDTLLRQMAATfpEAQILAAFGQTEmspvTCMLLGEDAIRKL--GSVGKVIPTVAARVVDENMNDVPVGEVGEI-----V 375
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 469 NRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSS 548
Cdd:PRK07786  376 YRAPTLMSGYWNNPEATAEAFAGGWFHSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGR 455
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 549 PDPIRGEVVKAFVVLNPDykshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKK 618
Cdd:PRK07786  456 ADEKWGEVPVAVAAVRND----DAALTLEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRER 521
FACL_like_2 cd05917
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
269-616 2.72e-48

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341241 [Multi-domain]  Cd Length: 349  Bit Score: 172.46  E-value: 2.72e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPKMTAHTHSSFglgLSvNGRF---WLDLTPSDVM----------------WNTSDTGwaksawssvfspw 329
Cdd:cd05917     7 IQFTSGTTGSPKGATLTHHNI---VN-NGYFigeRLGLTEQDRLcipvplfhcfgsvlgvLACLTHG------------- 69
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 330 iqGACVFTHhlPRFEPTSILQTLSKYPITVFCSAPTVY-RMLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGL- 407
Cdd:cd05917    70 --ATMVFPS--PSFDPLAVLEAIEKEKCTALHGVPTMFiAELEHPDFDKFDLSSLRTGIMAGAPCPPELMKRVIEVMNMk 145
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 408 DIYEGYGQTETVLICgnFKGMKIKP-----GSMGKPSPAFDVKIVDVNGNVLPP-GQEGDIGIqvlpnRPFGLFTHYVDN 481
Cdd:cd05917   146 DVTIAYGMTETSPVS--TQTRTDDSiekrvNTVGRIMPHTEAKIVDPEGGIVPPvGVPGELCI-----RGYSVMKGYWND 218
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 482 PSKTASTLRG-NFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAF 560
Cdd:cd05917   219 PEKTAEAIDGdGWLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAW 298
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1370468924 561 VVLNPDYKSHDQeqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:cd05917   299 IRLKEGAELTEE-----DIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
caiC PRK08008
putative crotonobetaine/carnitine-CoA ligase; Validated
72-616 1.60e-47

putative crotonobetaine/carnitine-CoA ligase; Validated


Pssm-ID: 181195 [Multi-domain]  Cd Length: 517  Bit Score: 174.49  E-value: 1.60e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  72 EKAGKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANiLSEACSLQRGDRVILILPRVPEW---WLAnVACLrtGTVLI 148
Cdd:PRK08008   15 DLADVYGHKTALIFESSGGVVRRYSYLELNEEINRTAN-LFYSLGIRKGDKVALHLDNCPEFifcWFG-LAKI--GAIMV 90
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 149 PGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCEN-LHSKLIVSENSREGWG-----NLKELMKVFLCHpgws 222
Cdd:PRK08008   91 PINARLLREESAWILQNSQASLLVTSAQFYPMYRQIQQEDATpLRHICLTRVALPADDGvssftQLKAQQPATLCY---- 166
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 223 tvarswltatstslvqaiLSLLSSWDyrhasdshtcvktkhneIMAIFFTSGTSGYPKMTAHTHSsfglglsvNGRF--- 299
Cdd:PRK08008  167 ------------------APPLSTDD-----------------TAEILFTSGTTSRPKGVVITHY--------NLRFagy 203
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 300 ---W-LDLTPSDVMWNTSdtgwaksawssvfsPWIQGACVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDI 375
Cdd:PRK08008  204 ysaWqCALRDDDVYLTVM--------------PAFHIDCQCTAAMAAFSAGATFVLLEKYSARAFWGQVCKYRATITECI 269
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 376 TSYKFKSLKHCVSAGE------------PITPDVTEKWRNKTGLDIYEGYGQTETVL-ICGNFKGMKIKPGSMGKPSPAF 442
Cdd:PRK08008  270 PMMIRTLMVQPPSANDrqhclrevmfylNLSDQEKDAFEERFGVRLLTSYGMTETIVgIIGDRPGDKRRWPSIGRPGFCY 349
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 443 DVKIVDVNGNVLPPGQEGDIGIQVLPNRPfgLFTHYVDNPSKTASTLRGNFYI-TGDRGYMDKDGYFWFVARADDVILSS 521
Cdd:PRK08008  350 EAEIRDDHNRPLPAGEIGEICIKGVPGKT--IFKEYYLDPKATAKVLEADGWLhTGDTGYVDEEGFFYFVDRRCNMIKRG 427
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 522 GYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDykshdqEQL-IKEIQEHVKKTTAPYKYPRKVEFIQ 600
Cdd:PRK08008  428 GENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEG------ETLsEEEFFAFCEQNMAKFKVPSYLEIRK 501
                         570
                  ....*....|....*.
gi 1370468924 601 ELPKTISGKTKRNELR 616
Cdd:PRK08008  502 DLPRNCSGKIIKKNLK 517
PRK12583 PRK12583
acyl-CoA synthetase; Provisional
91-616 2.06e-47

acyl-CoA synthetase; Provisional


Pssm-ID: 237145 [Multi-domain]  Cd Length: 558  Bit Score: 174.96  E-value: 2.06e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  91 EEMRWSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANC 170
Cdd:PRK12583   42 QALRYTWRQLADAVDRLARGLL-ALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRASELEYALGQSGVRW 120
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 171 IITNDvlapavdavASKCENLHSKL--IVSENSREGWGNLK-----ELMKVFLCHP-------GWSTVARSWLTATSTSL 236
Cdd:PRK12583  121 VICAD---------AFKTSDYHAMLqeLLPGLAEGQPGALAcerlpELRGVVSLAPapppgflAWHELQARGETVSREAL 191
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 237 VQAILSLlsswdyrhasdshtcvktKHNEIMAIFFTSGTSGYPKMTAHTHSSfglgLSVNGRF---WLDLTPSDVM---- 309
Cdd:PRK12583  192 AERQASL------------------DRDDPINIQYTSGTTGFPKGATLSHHN----ILNNGYFvaeSLGLTEHDRLcvpv 249
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 310 --WNTSDTGWAKSAWSSVfspwiqGACVFthhLPR--FEPTSILQTLSKYPITVFCSAPTVY-RMLVQNDITSYKFKSLK 384
Cdd:PRK12583  250 plYHCFGMVLANLGCMTV------GACLV---YPNeaFDPLATLQAVEEERCTALYGVPTMFiAELDHPQRGNFDLSSLR 320
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 385 HCVSAGEPITPDVTEKWRNKTGL-DIYEGYGQTET---VLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEG 460
Cdd:PRK12583  321 TGIMAGAPCPIEVMRRVMDEMHMaEVQIAYGMTETspvSLQTTAADDLERRVETVGRTQPHLEVKVVDPDGATVPRGEIG 400
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 461 DigiqvLPNRPFGLFTHYVDNPSKTASTLRGNFYI-TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPS 539
Cdd:PRK12583  401 E-----LCTRGYSVMKGYWNNPEATAESIDEDGWMhTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPA 475
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1370468924 540 VAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQeqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PRK12583  476 VADVQVFGVPDEKYGEEIVAWVRLHPGHAASEE-----ELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQKFRMR 547
FACL_like_4 cd05944
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
263-617 2.64e-47

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341266 [Multi-domain]  Cd Length: 359  Bit Score: 169.97  E-value: 2.64e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 263 HNEIMAIFFTSGTSGYPKMTAHTHSsfglGLSVNGrfW-----LDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGA-CVF 336
Cdd:cd05944     1 SDDVAAYFHTGGTTGTPKLAQHTHS----NEVYNA--WmlalnSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGAhVVL 74
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 337 THHLPRFEPT---SILQTLSKYPITVFCSAPTVYRMLVQNDITSyKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGY 413
Cdd:cd05944    75 AGPAGYRNPGlfdNFWKLVERYRITSLSTVPTVYAALLQVPVNA-DISSLRFAMSGAAPLPVELRARFEDATGLPVVEGY 153
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 414 GQTE-TVLICGNFKGMKIKPGSMGKPSPAFDVKIV--DVNGNVLPPGQEGDIGIQVLPNRpfGLFTHYVDNPSKTASTLR 490
Cdd:cd05944   154 GLTEaTCLVAVNPPDGPKRPGSVGLRLPYARVRIKvlDGVGRLLRDCAPDEVGEICVAGP--GVFGGYLYTEGNKNAFVA 231
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 491 GNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSh 570
Cdd:cd05944   232 DGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAVV- 310
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*..
gi 1370468924 571 DQEQLIKEIQEHVKKTTApykYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05944   311 EEEELLAWARDHVPERAA---VPKHIEVLEELPVTAVGKVFKPALRA 354
PRK05677 PRK05677
long-chain-fatty-acid--CoA ligase; Validated
96-621 7.37e-47

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 168170 [Multi-domain]  Cd Length: 562  Bit Score: 173.80  E-value: 7.37e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITND 175
Cdd:PRK05677   51 TYGELYKLSGAFAAWLQQHTDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLIVVNTNPLYTAREMEHQFNDSGAKALVCLA 130
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 VLAPAVDAVASKCENLHskLIVSEnsregWGNLKELMKVFLCHPGWSTVARswlTATSTSLVQAI--LSLLSswdyRHAS 253
Cdd:PRK05677  131 NMAHLAEKVLPKTGVKH--VIVTE-----VADMLPPLKRLLINAVVKHVKK---MVPAYHLPQAVkfNDALA----KGAG 196
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 254 DSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSfglgLSVNgrfWLDLTP--SDVMWNTSDTGWAKSAWSSVFSpwiq 331
Cdd:PRK05677  197 QPVTEANPQADDVAVLQYTGGTTGVAKGAMLTHRN----LVAN---MLQCRAlmGSNLNEGCEILIAPLPLYHIYA---- 265
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 332 gacvFTHHL---------------PRFEPtSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEPITP 395
Cdd:PRK05677  266 ----FTFHCmammlignhnilisnPRDLP-AMVKELGKWKFSGFVGLNTLFVALCNNeAFRKLDFSALKLTLSGGMALQL 340
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 396 DVTEKWRNKTGLDIYEGYGQTETV-LICGNFKGmKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQVLPNrp 471
Cdd:PRK05677  341 ATAERWKEVTGCAICEGYGMTETSpVVSVNPSQ-AIQVGTIGIPVPSTLCKVIDDDGNELPLGEVGELcvkGPQVMKG-- 417
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 472 fglfthYVDNPSKTASTLRGNFYI-TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPD 550
Cdd:PRK05677  418 ------YWQRPEATDEILDSDGWLkTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAALPGVLQCAAIGVPD 491
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1370468924 551 PIRGEVVKAFVVLNPDykshdqEQLIKE-IQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKEWK 621
Cdd:PRK05677  492 EKSGEAIKVFVVVKPG------ETLTKEqVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELRDEELK 557
PRK13382 PRK13382
bile acid CoA ligase;
269-618 1.52e-46

bile acid CoA ligase;


Pssm-ID: 172019 [Multi-domain]  Cd Length: 537  Bit Score: 172.25  E-value: 1.52e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPKMTAHTHSSFGLGLSVngrfWLDLTPsdvmWNTSDTGWAKSAwssVFSPWIQGACVFTHHLP------- 341
Cdd:PRK13382  201 ILLTSGTTGTPKGARRSGPGGIGTLKA----ILDRTP----WRAEEPTVIVAP---MFHAWGFSQLVLAASLActivtrr 269
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 342 RFEPTSILQTLSKYPITVFCSAPTVYRM---LVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTET 418
Cdd:PRK13382  270 RFDPEATLDLIDRHRATGLAVVPVMFDRimdLPAEVRNRYSGRSLRFAAASGSRMRPDVVIAFMDQFGDVIYNNYNATEA 349
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 419 VLIC-GNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGdiGIQVLPNRPFGLFThyvdnPSKTASTLRGnFYITG 497
Cdd:PRK13382  350 GMIAtATPADLRAAPDTAGRPAEGTEIRILDQDFREVPTGEVG--TIFVRNDTQFDGYT-----SGSTKDFHDG-FMASG 421
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 498 DRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPdykshDQEQLIK 577
Cdd:PRK13382  422 DVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKP-----GASATPE 496
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|.
gi 1370468924 578 EIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKK 618
Cdd:PRK13382  497 TLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQAR 537
PRK13390 PRK13390
acyl-CoA synthetase; Provisional
78-616 5.80e-46

acyl-CoA synthetase; Provisional


Pssm-ID: 139538 [Multi-domain]  Cd Length: 501  Bit Score: 169.80  E-value: 5.80e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  78 PSNPAFWwINRNGEEMrwSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQK 157
Cdd:PRK13390   11 PDRPAVI-VAETGEQV--SYRQLDDDSAALARVLYDA-GLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAP 86
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 158 DILYRLQSSKANCIitndVLAPAVDAVASKCENLHSKLIVSENSREGWGNLKelmkvflchpgwstvarSWLTATSTSLV 237
Cdd:PRK13390   87 EADYIVGDSGARVL----VASAALDGLAAKVGADLPLRLSFGGEIDGFGSFE-----------------AALAGAGPRLT 145
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 238 QailsllsswdyrhasdsHTCvktkhNEIMaiFFTSGTSGYPK-----MTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNT 312
Cdd:PRK13390  146 E-----------------QPC-----GAVM--LYSSGTTGFPKgiqpdLPGRDVDAPGDPIVAIARAFYDISESDIYYSS 201
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 313 SDTGWAKS-AWSSVFSPwIQGACVFTHhlpRFEPTSILQTLSKYPITVFCSAPTVY-RMLVQND--ITSYKFKSLKHCVS 388
Cdd:PRK13390  202 APIYHAAPlRWCSMVHA-LGGTVVLAK---RFDAQATLGHVERYRITVTQMVPTMFvRLLKLDAdvRTRYDVSSLRAVIH 277
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 389 AGEPITPDVTEKWRNKTGLDIYEGYGQTE----TVLICGNFKGmkiKPGSMGKpSPAFDVKIVDVNGNVLPPGQEGDIGI 464
Cdd:PRK13390  278 AAAPCPVDVKHAMIDWLGPIVYEYYSSTEahgmTFIDSPDWLA---HPGSVGR-SVLGDLHICDDDGNELPAGRIGTVYF 353
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 465 Q--VLPNRpfglfthYVDNPSKTASTLRGN--FYIT-GDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPS 539
Cdd:PRK13390  354 ErdRLPFR-------YLNDPEKTAAAQHPAhpFWTTvGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPA 426
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1370468924 540 VAESAVVSSPDPIRGEVVKAFVVLNPDYKShdQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PRK13390  427 VHDVAVIGVPDPEMGEQVKAVIQLVEGIRG--SDELARELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
PRK08751 PRK08751
long-chain fatty acid--CoA ligase;
96-616 8.59e-46

long-chain fatty acid--CoA ligase;


Pssm-ID: 181546 [Multi-domain]  Cd Length: 560  Bit Score: 170.44  E-value: 8.59e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITND 175
Cdd:PRK08751   52 TYREADQLVEQFAAYLLGELQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVID 131
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 VLAPAVDAVASKCEnlhskliVSENSREGWGNLKELMKVFLCHPGWSTVAR---SWLTATSTSLVQAiLSLLSswdyRHa 252
Cdd:PRK08751  132 NFGTTVQQVIADTP-------VKQVITTGLGDMLGFPKAALVNFVVKYVKKlvpEYRINGAIRFREA-LALGR----KH- 198
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 253 sdSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRfWLDLTpsdvmwNTSDTG-------------WAK 319
Cdd:PRK08751  199 --SMPTLQIEPDDIAFLQYTGGTTGVAKGAMLTHRNLVANMQQAHQ-WLAGT------GKLEEGcevvitalplyhiFAL 269
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 320 SAWSSVFSPWiqGACvftHHL---PRFEPTSIlQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEPITP 395
Cdd:PRK08751  270 TANGLVFMKI--GGC---NHLisnPRDMPGFV-KELKKTRFTAFTGVNTLFNGLLNTpGFDQIDFSSLKMTLGGGMAVQR 343
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 396 DVTEKWRNKTGLDIYEGYGQTETV-LICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQVLPNrp 471
Cdd:PRK08751  344 SVAERWKQVTGLTLVEAYGLTETSpAACINPLTLKEYNGSIGLPIPSTDACIKDDAGTVLAIGEIGELcikGPQVMKG-- 421
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 472 fglfthYVDNPSKTASTLRGNFYI-TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPD 550
Cdd:PRK08751  422 ------YWKRPEETAKVMDADGWLhTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAVGVPD 495
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1370468924 551 PIRGEVVKAFVVlnpdykSHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PRK08751  496 EKSGEIVKVVIV------KKDPALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRRELR 555
PLN02654 PLN02654
acetate-CoA ligase
83-617 1.03e-45

acetate-CoA ligase


Pssm-ID: 215353 [Multi-domain]  Cd Length: 666  Bit Score: 172.00  E-value: 1.03e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  83 FWWINRNGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYR 162
Cdd:PLN02654  109 YWEGNEPGFDASLTYSELLDRVCQLANYL-KDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAESLAQR 187
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 163 LQSSKANCIIT-NDV--------LAPAVDAVASKCENLHSKL---IVSENS----REG--WGNLKELMkvflchpgWSTV 224
Cdd:PLN02654  188 IVDCKPKVVITcNAVkrgpktinLKDIVDAALDESAKNGVSVgicLTYENQlamkREDtkWQEGRDVW--------WQDV 259
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 225 ARSWLTATSTSLVQAilsllsswdyrhasdshtcvktkhNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLT 304
Cdd:PLN02654  260 VPNYPTKCEVEWVDA------------------------EDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYK 315
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 305 PSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTHH-LPRF-EPTSILQTLSKYPITVFCSAPTVYRMLVQND---ITSYK 379
Cdd:PLN02654  316 PTDVYWCTADCGWITGHSYVTYGPMLNGATVLVFEgAPNYpDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGdeyVTRHS 395
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 380 FKSLKHCVSAGEPITPDVTEKWRNKTG---LDIYEGYGQTETvlicGNFKGMKI------KPGSMGKPSPAFDVKIVDVN 450
Cdd:PLN02654  396 RKSLRVLGSVGEPINPSAWRWFFNVVGdsrCPISDTWWQTET----GGFMITPLpgawpqKPGSATFPFFGVQPVIVDEK 471
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 451 GNVLPPGQEGDIGIQvlPNRPFGLFTHYVDNPSKTASTLR--GNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPF 528
Cdd:PLN02654  472 GKEIEGECSGYLCVK--KSWPGAFRTLYGDHERYETTYFKpfAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTA 549
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 529 EVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKShdQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISG 608
Cdd:PLN02654  550 EVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVEGVPY--SEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSG 627

                  ....*....
gi 1370468924 609 KTKRNELRK 617
Cdd:PLN02654  628 KIMRRILRK 636
BACL_like cd05929
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ...
340-616 1.07e-45

Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.


Pssm-ID: 341252 [Multi-domain]  Cd Length: 473  Bit Score: 168.71  E-value: 1.07e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 340 LPRFEPTSILQTLSKYPITVFCSAPTVY-RML-----VQNditSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGY 413
Cdd:cd05929   200 MEKFDPEEFLRLIERYRVTFAQFVPTMFvRLLklpeaVRN---AYDLSSLKRVIHAAAPCPPWVKEQWIDWGGPIIWEYY 276
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 414 GQTETV-LICGNFKGMKIKPGSMGKPSPAfDVKIVDVNGNVLPPGQEGDIgiQVLPNRPFglftHYVDNPSKTA-STLRG 491
Cdd:cd05929   277 GGTEGQgLTIINGEEWLTHPGSVGRAVLG-KVHILDEDGNEVPPGEIGEV--YFANGPGF----EYTNDPEKTAaARNEG 349
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 492 NFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAfvVLNPDYKSHD 571
Cdd:cd05929   350 GWSTLGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHA--VVQPAPGADA 427
                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*
gi 1370468924 572 QEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:cd05929   428 GTALAEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLR 472
FACL_like_6 cd05922
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
213-616 1.24e-45

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341246 [Multi-domain]  Cd Length: 457  Bit Score: 168.00  E-value: 1.24e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 213 KVFLCHPGWSTVARSWLTATSTSLVqaILSLLSSWDYRHASDSHTCVktkHNEIMAIFFTSGTSGYPKMTAHTHSSFGLG 292
Cdd:cd05922    71 RIVLADAGAADRLRDALPASPDPGT--VLDADGIRAARASAPAHEVS---HEDLALLLYTSGSTGSPKLVRLSHQNLLAN 145
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 293 L-SVNGRfwLDLTPSDVMWNTSDTGWAkSAWSSVFSPWIQGACVFTHHLPRFePTSILQTLSKYPITVFCSAPTVYRMLV 371
Cdd:cd05922   146 ArSIAEY--LGITADDRALTVLPLSYD-YGLSVLNTHLLRGATLVLTNDGVL-DDAFWEDLREHGATGLAGVPSTYAMLT 221
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 372 QNDITSYKFKSLKHCVSAGEPITPDVTEKWRNK-TGLDIYEGYGQTETvlicgnFKGMKI--------KPGSMGKPSPAF 442
Cdd:cd05922   222 RLGFDPAKLPSLRYLTQAGGRLPQETIARLRELlPGAQVYVMYGQTEA------TRRMTYlpperileKPGSIGLAIPGG 295
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 443 DVKIVDVNGNVLPPGQEGDIGiqvlPNRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSG 522
Cdd:cd05922   296 EFEILDDDGTPTPPGEPGEIV----HRGPNVMKGYWNDPPYRRKEGRGGGVLHTGDLARRDEDGFLFIVGRRDRMIKLFG 371
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 523 YRIGPFEVENALNEHPSVAESAVVSSPDPIrGEVVKAFVVLNPDYKSHDqeqlikeIQEHVKKTTAPYKYPRKVEFIQEL 602
Cdd:cd05922   372 NRISPTEIEAAARSIGLIIEAAAVGLPDPL-GEKLALFVTAPDKIDPKD-------VLRSLAERLPPYKVPATVRVVDEL 443
                         410
                  ....*....|....
gi 1370468924 603 PKTISGKTKRNELR 616
Cdd:cd05922   444 PLTASGKVDYAALR 457
PRK12406 PRK12406
long-chain-fatty-acid--CoA ligase; Provisional
341-620 7.25e-45

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 183506 [Multi-domain]  Cd Length: 509  Bit Score: 167.18  E-value: 7.25e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 341 PRFEPTSILQTLSKYPITVFCSAPTVY-RML-----VQNditSYKFKSLKHCVSAGEPITPDVT----EKWrnktGLDIY 410
Cdd:PRK12406  228 PRFDPEELLQLIERHRITHMHMVPTMFiRLLklpeeVRA---KYDVSSLRHVIHAAAPCPADVKramiEWW----GPVIY 300
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 411 EGYGQTET--VLICGNFKGMKiKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVLPNrpfGLFThYVDNPSKTAST 488
Cdd:PRK12406  301 EYYGSTESgaVTFATSEDALS-HPGTVGKAAPGAELRFVDEDGRPLPQGEIGEIYSRIAGN---PDFT-YHNKPEKRAEI 375
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 489 LRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyK 568
Cdd:PRK12406  376 DRGGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEPQPG-A 454
                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1370468924 569 SHDQEqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKEW 620
Cdd:PRK12406  455 TLDEA----DIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLRDPYW 502
DltA cd05945
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ...
264-615 7.55e-45

D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341267 [Multi-domain]  Cd Length: 449  Bit Score: 165.50  E-value: 7.55e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 264 NEIMAIFFTSGTSGYPKMTAHTHS---SFGLGLsvNGRFwlDLTPSDVMWNTSDtgwaksaWS---SVFS---PWIQGAC 334
Cdd:cd05945    97 DDNAYIIFTSGSTGRPKGVQISHDnlvSFTNWM--LSDF--PLGPGDVFLNQAP-------FSfdlSVMDlypALASGAT 165
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 335 VFThhLPRFE---PTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEPITPDVTEKWRNKT-GLDI 409
Cdd:cd05945   166 LVP--VPRDAtadPKQLFRFLAEHGITVWVSTPSFAAMCLLSpTFTPESLPSLRHFLFCGEVLPHKTARALQQRFpDARI 243
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 410 YEGYGQTETVLICgnfKGMKIKPGSM--------GKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQVlpnrpfglFTHY 478
Cdd:cd05945   244 YNTYGPTEATVAV---TYIEVTPEVLdgydrlpiGYAKPGAKLVILDEDGRPVPPGEKGELvisGPSV--------SKGY 312
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 479 VDNPSKTASTLRGNF----YITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRG 554
Cdd:cd05945   313 LNNPEKTAAAFFPDEgqraYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKV 392
                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1370468924 555 EVVKAFVVLNPdyksHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd05945   393 TELIAFVVPKP----GAEAGLTKAIKAELAERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
ttLC_FACS_AEE21_like cd12118
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ...
92-617 1.60e-44

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.


Pssm-ID: 341283 [Multi-domain]  Cd Length: 486  Bit Score: 165.55  E-value: 1.60e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  92 EMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCI 171
Cdd:cd12118    27 DRRYTWRQTYDRCRRLASALAAL-GISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRLDAEEIAFILRHSEAKVL 105
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 172 IT------NDVLApavdavaskcenlhsklivsensrEGWGNlkelmkvflchPGWSTVARSWltatstslvQAIlslls 245
Cdd:cd12118   106 FVdrefeyEDLLA------------------------EGDPD-----------FEWIPPADEW---------DPI----- 136
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 246 swdyrhasdshtcvktkhneimAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWlDLTPSDV-MWNTSD---TGWAksa 321
Cdd:cd12118   137 ----------------------ALNYTSGTTGRPKGVVYHHRGAYLNALANILEW-EMKQHPVyLWTLPMfhcNGWC--- 190
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 322 wssvfSPWIQGACVFTHH-LPRFEPTSILQTLSKYPITVFCSAPTVYRMLVqNDITSYKfKSLKHCVS---AGEPITPDV 397
Cdd:cd12118   191 -----FPWTVAAVGGTNVcLRKVDAKAIYDLIEKHKVTHFCGAPTVLNMLA-NAPPSDA-RPLPHRVHvmtAGAPPPAAV 263
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 398 TEKWRNKtGLDIYEGYGQTET---VLICgnfkgmKIKPGSMGKPSP---------------AFDVKIVDVNGNVLPPGQE 459
Cdd:cd12118   264 LAKMEEL-GFDVTHVYGLTETygpATVC------AWKPEWDELPTEerarlkarqgvryvgLEEVDVLDPETMKPVPRDG 336
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 460 GDIGIQVLpnRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPS 539
Cdd:cd12118   337 KTIGEIVF--RGNIVMKGYLKNPEATAEAFRGGWFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPA 414
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1370468924 540 VAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQeqlikEIQEHVKKTTAPYKYPRKVEFiQELPKTISGKTKRNELRK 617
Cdd:cd12118   415 VLEAAVVARPDEKWGEVPCAFVELKEGAKVTEE-----EIIAFCREHLAGFMVPKTVVF-GELPKTSTGKIQKFVLRD 486
PRK07787 PRK07787
acyl-CoA synthetase; Validated
338-616 5.37e-44

acyl-CoA synthetase; Validated


Pssm-ID: 236096 [Multi-domain]  Cd Length: 471  Bit Score: 163.62  E-value: 5.37e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 338 HHLPRFEPTSILQTLSKyPITVFCSAPTVYRMLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTE 417
Cdd:PRK07787  199 VHTGRPTPEAYAQALSE-GGTLYFGVPTVWSRIAADPEAARALRGARLLVSGSAALPVPVFDRLAALTGHRPVERYGMTE 277
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 418 TVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVlpnRPFGLFTHYVDNPSKTASTLRGN-FYIT 496
Cdd:PRK07787  278 TLITLSTRADGERRPGWVGLPLAGVETRLVDEDGGPVPHDGETVGELQV---RGPTLFDGYLNRPDATAAAFTADgWFRT 354
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 497 GDRGYMDKDGYFWFVAR-ADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYkshDQEQL 575
Cdd:PRK07787  355 GDVAVVDPDGMHRIVGReSTDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDV---AADEL 431
                         250       260       270       280
                  ....*....|....*....|....*....|....*....|.
gi 1370468924 576 IkeiqEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PRK07787  432 I----DFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLL 468
ttLC_FACS_AlkK_like cd12119
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
90-617 1.14e-43

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.


Pssm-ID: 341284 [Multi-domain]  Cd Length: 518  Bit Score: 163.96  E-value: 1.14e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  90 GEEMRWSFEELGSLSRKFANILSEAcSLQRGDRVILIL---PRVPEWWLAnVAClrTGTVLIPGTTQLTQKDILYRLQSS 166
Cdd:cd12119    21 GEVHRYTYAEVAERARRLANALRRL-GVKPGDRVATLAwntHRHLELYYA-VPG--MGAVLHTINPRLFPEQIAYIINHA 96
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 167 KANCIITNDVLAPAVDAVASKcenlhsklivsensregwgnLKELMKVFLchpgwstvarswLTATSTSLVQAILSLLSS 246
Cdd:cd12119    97 EDRVVFVDRDFLPLLEAIAPR--------------------LPTVEHVVV------------MTDDAAMPEPAGVGVLAY 144
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 247 WDYRHA-SDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLG-LSVNGRFWLDLTPSDV------MWNTSdtgwa 318
Cdd:cd12119   145 EELLAAeSPEYDWPDFDENTAAAICYTSGTTGNPKGVVYSHRSLVLHaMAALLTDGLGLSESDVvlpvvpMFHVN----- 219
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 319 ksAWSSVFSPWIQGAC-VFTHhlPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEPITPD 396
Cdd:cd12119   220 --AWGLPYAAAMVGAKlVLPG--PYLDPASLAELIEREGVTFAAGVPTVWQGLLDHlEANGRDLSSLRRVVIGGSAVPRS 295
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 397 VTEKWRNKtGLDIYEGYGQTET--VLICGnfkgmKIKPG--------------SMGKPSPAFDVKIVDVNGNVLP--PGQ 458
Cdd:cd12119   296 LIEAFEER-GVRVIHAWGMTETspLGTVA-----RPPSEhsnlsedeqlalraKQGRPVPGVELRIVDDDGRELPwdGKA 369
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 459 EGDIgiQVlpnR-PFgLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEH 537
Cdd:cd12119   370 VGEL--QV---RgPW-VTKSYYKNDEESEALTEDGWLRTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAH 443
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 538 PSVAESAVVSSPDPIRGEVVKAFVVLNPdykshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd12119   444 PAVAEAAVIGVPHPKWGERPLAVVVLKE-----GATVTAEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKALRE 518
PRK07788 PRK07788
acyl-CoA synthetase; Validated
261-619 3.10e-43

acyl-CoA synthetase; Validated


Pssm-ID: 236097 [Multi-domain]  Cd Length: 549  Bit Score: 163.17  E-value: 3.10e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 261 TKHNEImaIFFTSGTSGYPKMTAHTHSSfglGLSVNGRFwLDLTP---SDVMWNTS----DTGWAKSAWSSVFspwiqGA 333
Cdd:PRK07788  206 PKPGGI--VILTSGTTGTPKGAPRPEPS---PLAPLAGL-LSRVPfraGETTLLPApmfhATGWAHLTLAMAL-----GS 274
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 334 CVFTHHlpRFEPTSILQTLSKYPITVFCSAPT-VYRML--VQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIY 410
Cdd:PRK07788  275 TVVLRR--RFDPEATLEDIAKHKATALVVVPVmLSRILdlGPEVLAKYDTSSLKIIFVSGSALSPELATRALEAFGPVLY 352
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 411 EGYGQTE----TVlicGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKta 486
Cdd:PRK07788  353 NLYGSTEvafaTI---ATPEDLAEAPGTVGRPPKGVTVKILDENGNEVPRGVVGRIFV-----GNGFPFEGYTDGRDK-- 422
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 487 STLRGnFYITGDRGYMDKDGYfWFVA-RADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNP 565
Cdd:PRK07788  423 QIIDG-LLSSGDVGYFDEDGL-LFVDgRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAP 500
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1370468924 566 DyKSHDQEqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKE 619
Cdd:PRK07788  501 G-AALDED----AIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREMD 549
PRK13391 PRK13391
acyl-CoA synthetase; Provisional
78-621 7.51e-43

acyl-CoA synthetase; Provisional


Pssm-ID: 184022 [Multi-domain]  Cd Length: 511  Bit Score: 161.40  E-value: 7.51e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  78 PSNPAFWwINRNGEEMrwSFEELGSLSRKFANILSEAcSLQRGDRVILIL---PRVPEwwlANVACLRTGTVLIPGTTQL 154
Cdd:PRK13391   11 PDKPAVI-MASTGEVV--TYRELDERSNRLAHLFRSL-GLKRGDHVAIFMennLRYLE---VCWAAERSGLYYTCVNSHL 83
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 155 TQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIV-SENSREGWGNLKElmkvflchpgwsTVARswLTATs 233
Cdd:PRK13391   84 TPAEAAYIVDDSGARALITSAAKLDVARALLKQCPGVRHRLVLdGDGELEGFVGYAE------------AVAG--LPAT- 148
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 234 tslvqailsllsswdyRHASDShtcvktkhnEIMAIFFTSGTSGYPK----------------MTAHTHSSFGLGlsvNG 297
Cdd:PRK13391  149 ----------------PIADES---------LGTDMLYSSGTTGRPKgikrplpeqppdtplpLTAFLQRLWGFR---SD 200
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 298 RFWLDLTPsdvMWNTsdtgwAKSAWSSVfspwIQ--GACVFThhLPRFEPTSILQTLSKYPITVFCSAPTVY-RML-VQN 373
Cdd:PRK13391  201 MVYLSPAP---LYHS-----APQRAVML----VIrlGGTVIV--MEHFDAEQYLALIEEYGVTHTQLVPTMFsRMLkLPE 266
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 374 DI-TSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETVLICG-NFKGMKIKPGSMGKPSPAfDVKIVDVNG 451
Cdd:PRK13391  267 EVrDKYDLSSLEVAIHAAAPCPPQVKEQMIDWWGPIIHEYYAATEGLGFTAcDSEEWLAHPGTVGRAMFG-DLHILDDDG 345
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 452 NVLPPGQEGDIGIQvlPNRPFglftHYVDNPSKTASTL--RGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFE 529
Cdd:PRK13391  346 AELPPGEPGTIWFE--GGRPF----EYLNDPAKTAEARhpDGTWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQE 419
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 530 VENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNP--DYKSHDQEQLIKEIQEHVkkttAPYKYPRKVEFIQELPKTIS 607
Cdd:PRK13391  420 AENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDgvDPGPALAAELIAFCRQRL----SRQKCPRSIDFEDELPRLPT 495
                         570
                  ....*....|....
gi 1370468924 608 GKTKRNELRKKEWK 621
Cdd:PRK13391  496 GKLYKRLLRDRYWG 509
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
96-545 1.34e-42

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 158.20  E-value: 1.34e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPgttqLtqkDILY---RLQS----SKA 168
Cdd:TIGR01733   1 TYRELDERANRLARHLRAAGGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVP----L---DPAYpaeRLAFiledAGA 73
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 169 NCIITNDVLAPAVDAVASKCENlhsklivsensregwgnlkelmkvflchpgwstVARSWLTATSTSLVqailsllsswd 248
Cdd:TIGR01733  74 RLLLTDSALASRLAGLVLPVIL---------------------------------LDPLELAALDDAPA----------- 109
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 249 yrhasDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWlDLTPSDVMWNTSDTGWAKSAWSsVFSP 328
Cdd:TIGR01733 110 -----PPPPDAPSGPDDLAYVIYTSGSTGRPKGVVVTHRSLVNLLAWLARRY-GLDPDDRVLQFASLSFDASVEE-IFGA 182
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 329 WIQGACVF--THHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSykFKSLKHCVSAGEPITPDVTEKWRNKTG 406
Cdd:TIGR01733 183 LLAGATLVvpPEDEERDDAALLAALIAEHPVTVLNLTPSLLALLAAALPPA--LASLRLVILGGEALTPALVDRWRARGP 260
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 407 -LDIYEGYGQTETVLICGnfkgMKIKPGSM---------GKPSPAFDVKIVDVNGNVLPPGQEGDIGIqvlpnRPFGLFT 476
Cdd:TIGR01733 261 gARLINLYGPTETTVWST----ATLVDPDDaprespvpiGRPLANTRLYVLDDDLRPVPVGVVGELYI-----GGPGVAR 331
                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1370468924 477 HYVDNPSKTA---------STLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAV 545
Cdd:TIGR01733 332 GYLNRPELTAerfvpdpfaGGDGARLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
PtmA cd17636
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ...
332-608 1.73e-41

long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341291 [Multi-domain]  Cd Length: 331  Bit Score: 153.23  E-value: 1.73e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 332 GACVFthhLPRFEPTSILQTLSKYPIT-VFCSAPTVYRMLVQNDITSYKFKSLKHCVSAGE---PITPDVTEkWRNKTGl 407
Cdd:cd17636    67 GTNVF---VRRVDAEEVLELIEAERCThAFLLPPTIDQIVELNADGLYDLSSLRSSPAAPEwndMATVDTSP-WGRKPG- 141
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 408 diyeGYGQTETV-LICGNFKGMKIKpGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTA 486
Cdd:cd17636   142 ----GYGQTEVMgLATFAALGGGAI-GGAGRPSPLVQVRILDEDGREVPDGEVGEIVA-----RGPTVMAGYWNRPEVNA 211
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 487 STLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPD 566
Cdd:cd17636   212 RRTRGGWHHTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPG 291
                         250       260       270       280
                  ....*....|....*....|....*....|....*....|..
gi 1370468924 567 yKSHDQEQLIkeiqEHVKKTTAPYKYPRKVEFIQELPKTISG 608
Cdd:cd17636   292 -ASVTEAELI----EHCRARIASYKKPKSVEFADALPRTAGG 328
FadD3 cd17638
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ...
265-609 4.52e-41

acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.


Pssm-ID: 341293 [Multi-domain]  Cd Length: 330  Bit Score: 151.88  E-value: 4.52e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 265 EIMAIFFTSGTSGYPK--MTAHTHSsfgLGLSVNgrfWldltpSDVMWNTSDTGWA-----------KSAWSSVFspwIQ 331
Cdd:cd17638     1 DVSDIMFTSGTTGRSKgvMCAHRQT---LRAAAA---W-----ADCADLTEDDRYLiinpffhtfgyKAGIVACL---LT 66
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 332 GACVFTHHLprFEPTSILQTLSKYPITVFCSAPTVYR-MLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLD-I 409
Cdd:cd17638    67 GATVVPVAV--FDVDAILEAIERERITVLPGPPTLFQsLLDHPGRKKFDLSSLRAAVTGAATVPVELVRRMRSELGFEtV 144
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 410 YEGYGQTE--TVLICGNFKGMKIKPGSMGKPSPAFDVKIVDvNGNVLPPGqegdigiqvlPNRPFGlfthYVDNPSKTAS 487
Cdd:cd17638   145 LTAYGLTEagVATMCRPGDDAETVATTCGRACPGFEVRIAD-DGEVLVRG----------YNVMQG----YLDDPEATAE 209
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 488 TLRGNFYI-TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPD 566
Cdd:cd17638   210 AIDADGWLhTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPG 289
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|...
gi 1370468924 567 yKSHDQEQLIKEIQEHVkkttAPYKYPRKVEFIQELPKTISGK 609
Cdd:cd17638   290 -VTLTEEDVIAWCRERL----ANYKVPRFVRFLDELPRNASGK 327
PRK13295 PRK13295
cyclohexanecarboxylate-CoA ligase; Reviewed
67-616 2.18e-40

cyclohexanecarboxylate-CoA ligase; Reviewed


Pssm-ID: 171961 [Multi-domain]  Cd Length: 547  Bit Score: 154.83  E-value: 2.18e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  67 QWTDK-------EKAGKKPSNPAFWWINRNGEEM-RWSFEELGSLSRKFANILSEaCSLQRGDRVILilpRVPEWWLANV 138
Cdd:PRK13295   20 HWHDRtinddldACVASCPDKTAVTAVRLGTGAPrRFTYRELAALVDRVAVGLAR-LGVGRGDVVSC---QLPNWWEFTV 95
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 139 ---ACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIItndvlAPAV----DAVAskcenlhskliVSENSREGWGNLKEL 211
Cdd:PRK13295   96 lylACSRIGAVLNPLMPIFRERELSFMLKHAESKVLV-----VPKTfrgfDHAA-----------MARRLRPELPALRHV 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 212 MKV----------FLCHPGWSTVARSwltatstslvQAILSllsswdyRHASDShtcvktkhNEIMAIFFTSGTSGYPKM 281
Cdd:PRK13295  160 VVVggdgadsfeaLLITPAWEQEPDA----------PAILA-------RLRPGP--------DDVTQLIYTSGTTGEPKG 214
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 282 TAHTHSS-FGLGLSVNGRfwLDLTPSDVMWNTS----DTGWAKSAwssvFSPWIQGACVFTHHLprFEPTSILQTLSKYP 356
Cdd:PRK13295  215 VMHTANTlMANIVPYAER--LGLGADDVILMASpmahQTGFMYGL----MMPVMLGATAVLQDI--WDPARAAELIRTEG 286
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 357 ITvFCSAPTVYRM-LVQN-DITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETVLICGnfkgmkIKPG- 433
Cdd:PRK13295  287 VT-FTMASTPFLTdLTRAvKESGRPVSSLRTFLCAGAPIPGALVERARAALGAKIVSAWGMTENGAVTL------TKLDd 359
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 434 -------SMGKPSPAFDVKIVDVNGNVLPPGQEGDIgiQVlpnRPFGLFTHYVDNPSKTASTLRGnFYITGDRGYMDKDG 506
Cdd:PRK13295  360 pderastTDGCPLPGVEVRVVDADGAPLPAGQIGRL--QV---RGCSNFGGYLKRPQLNGTDADG-WFDTGDLARIDADG 433
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 507 YFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyKSHDQEQLIKEIQEHvkKT 586
Cdd:PRK13295  434 YIRISGRSKDVIIRGGENIPVVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVPRPG-QSLDFEEMVEFLKAQ--KV 510
                         570       580       590
                  ....*....|....*....|....*....|
gi 1370468924 587 TAPYkYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PRK13295  511 AKQY-IPERLVVRDALPRTPSGKIQKFRLR 539
PLN02246 PLN02246
4-coumarate--CoA ligase
72-618 2.55e-40

4-coumarate--CoA ligase


Pssm-ID: 215137 [Multi-domain]  Cd Length: 537  Bit Score: 154.75  E-value: 2.55e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  72 EKAGKKPSNPAFwwIN-RNGEEMrwSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPG 150
Cdd:PLN02246   31 ERLSEFSDRPCL--IDgATGRVY--TYADVELLSRRVAAGLH-KLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTA 105
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 151 TTQLTQKDILYRLQSSKANCIITndvLAPAVDAVASKCENLHSKLIVSENSREGWGNLKELMkvflchpgwstvarswlt 230
Cdd:PLN02246  106 NPFYTPAEIAKQAKASGAKLIIT---QSCYVDKLKGLAEDDGVTVVTIDDPPEGCLHFSELT------------------ 164
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 231 atstslvqailsllsswdyrhASDSHTC--VKTKHNEIMAIFFTSGTSGYPKMTAHTHSsfGLGLSV---------Ngrf 299
Cdd:PLN02246  165 ---------------------QADENELpeVEISPDDVVALPYSSGTTGLPKGVMLTHK--GLVTSVaqqvdgenpN--- 218
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 300 wLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFThhLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDIT-SY 378
Cdd:PLN02246  219 -LYFHSDDVILCVLPMFHIYSLNSVLLCGLRVGAAILI--MPKFEIGALLELIQRHKVTIAPFVPPIVLAIAKSPVVeKY 295
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 379 KFKSLKHCVSAGEPITPDVTEKWRNKTGLDIY-EGYGQTE--TVL-ICGNF--KGMKIKPGSMGKPSPAFDVKIVDVN-G 451
Cdd:PLN02246  296 DLSSIRMVLSGAAPLGKELEDAFRAKLPNAVLgQGYGMTEagPVLaMCLAFakEPFPVKSGSCGTVVRNAELKIVDPEtG 375
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 452 NVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTL-RGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEV 530
Cdd:PLN02246  376 ASLPRNQPGEICI-----RGPQIMKGYLNDPEATANTIdKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAEL 450
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 531 ENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYK-SHDqeqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGK 609
Cdd:PLN02246  451 EALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGSEiTED------EIKQFVAKQVVFYKRIHKVFFVDSIPKAPSGK 524

                  ....*....
gi 1370468924 610 TKRNELRKK 618
Cdd:PLN02246  525 ILRKDLRAK 533
AAS_C cd05909
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ...
258-617 1.35e-39

C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.


Pssm-ID: 341235 [Multi-domain]  Cd Length: 490  Bit Score: 151.72  E-value: 1.35e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 258 CVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGL-SVNGRFwlDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVF 336
Cdd:cd05909   141 VAPVQPDDPAVILFTSGSEGLPKGVVLSHKNLLANVeQITAIF--DPNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKVV 218
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 337 THHLPrFEPTSILQTLSKYPITVFCSAPTVYRMLVQNdITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQT 416
Cdd:cd05909   219 FHPNP-LDYKKIPELIYDKKATILLGTPTFLRGYARA-AHPEDFSSLRLVVAGAEKLKDTLRQEFQEKFGIRILEGYGTT 296
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 417 ETV-LICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNV-LPPGQEGDIGIQVlPNrpfgLFTHYVDNPSKTASTLRGNFY 494
Cdd:cd05909   297 ECSpVISVNTPQSPNKEGTVGRPLPGMEVKIVSVETHEeVPIGEGGLLLVRG-PN----VMLGYLNEPELTSFAFGDGWY 371
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 495 ITGDRGYMDKDGYFWFVARaddviLSSGYRIG----PFE-VENALNEH-PSVAESAVVSSPDPIRGEVVKAFVVLnpdyk 568
Cdd:cd05909   372 DTGDIGKIDGEGFLTITGR-----LSRFAKIAgemvSLEaIEDILSEIlPEDNEVAVVSVPDGRKGEKIVLLTTT----- 441
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|
gi 1370468924 569 sHDQEQLikEIQEHVKKTTAPYKY-PRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05909   442 -TDTDPS--SLNDILKNAGISNLAkPSYIHQVEEIPLLGTGKPDYVTLKA 488
PRK12492 PRK12492
long-chain-fatty-acid--CoA ligase; Provisional
96-616 5.57e-39

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 171539 [Multi-domain]  Cd Length: 562  Bit Score: 151.13  E-value: 5.57e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITND 175
Cdd:PRK12492   51 SYAELERHSAAFAAYLQQHTDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTAREMRHQFKDSGARALVYLN 130
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 VLAPAVDAVASKCENLHskLIVSEnsregwgnLKELMkvflchpgwsTVARSWLTATSTSLVQAIL---SLLSSWDYRHA 252
Cdd:PRK12492  131 MFGKLVQEVLPDTGIEY--LIEAK--------MGDLL----------PAAKGWLVNTVVDKVKKMVpayHLPQAVPFKQA 190
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 253 -----SDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSfglgLSVN--------GRFWLDLTPsdVMWNTSDTGWAK 319
Cdd:PRK12492  191 lrqgrGLSLKPVPVGLDDIAVLQYTGGTTGLAKGAMLTHGN----LVANmlqvraclSQLGPDGQP--LMKEGQEVMIAP 264
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 320 SAWSSVFSPWIQGACVF---THHLPRFEPTSI---LQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEP 392
Cdd:PRK12492  265 LPLYHIYAFTANCMCMMvsgNHNVLITNPRDIpgfIKELGKWRFSALLGLNTLFVALMDHpGFKDLDFSALKLTNSGGTA 344
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 393 ITPDVTEKWRNKTGLDIYEGYGQTETV-LICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQVLP 468
Cdd:PRK12492  345 LVKATAERWEQLTGCTIVEGYGLTETSpVASTNPYGELARLGTVGIPVPGTALKVIDDDGNELPLGERGELcikGPQVMK 424
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 469 NrpfglfthYVDNPSKTASTLRGN-FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVS 547
Cdd:PRK12492  425 G--------YWQQPEATAEALDAEgWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVMAHPKVANCAAIG 496
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1370468924 548 SPDPIRGEVVKAFVVlnpdykSHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PRK12492  497 VPDERSGEAVKLFVV------ARDPGLSVEELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRRELR 559
Firefly_Luc cd17642
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ...
92-617 9.27e-39

insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341297 [Multi-domain]  Cd Length: 532  Bit Score: 149.98  E-value: 9.27e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  92 EMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCI 171
Cdd:cd17642    42 GVNYSYAEYLEMSVRLAEALKKY-GLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIV 120
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 172 ITNDVLAPAVDAVASKceNLHSKLIVSENSREGWGNLKELMKvFLCHPGWSTVARSWLTATSTSlvqailsllsswdyrh 251
Cdd:cd17642   121 FCSKKGLQKVLNVQKK--LKIIKTIIILDSKEDYKGYQCLYT-FITQNLPPGFNEYDFKPPSFD---------------- 181
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 252 asdshtcvktKHNEIMAIFFTSGTSGYPKMTAHTHssfglgLSVNGRFWLDLTPSDVMWNTSDTgwaksAWSSVFsPWIQ 331
Cdd:cd17642   182 ----------RDEQVALIMNSSGSTGLPKGVQLTH------KNIVARFSHARDPIFGNQIIPDT-----AILTVI-PFHH 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 332 GACVFTH-----------HLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITS-YKFKSLKHCVSAGEPITPDVTE 399
Cdd:cd17642   240 GFGMFTTlgylicgfrvvLMYKFEEELFLRSLQDYKVQSALLVPTLFAFFAKSTLVDkYDLSNLHEIASGGAPLSKEVGE 319
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 400 KWRNKTGLD-IYEGYGQTET---VLICGNfkgMKIKPGSMGKPSPAFDVKIVDVN-GNVLPPGQEGDIGIqvlpnRPFGL 474
Cdd:cd17642   320 AVAKRFKLPgIRQGYGLTETtsaILITPE---GDDKPGAVGKVVPFFYAKVVDLDtGKTLGPNERGELCV-----KGPMI 391
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 475 FTHYVDNPSKTASTLRGNFYI-TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIR 553
Cdd:cd17642   392 MKGYVNNPEATKALIDKDGWLhSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDA 471
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1370468924 554 GEVVKAFVVLnpdykSHDQEQLIKEIQEHVKKTTAPYKYPR-KVEFIQELPKTISGKTKRNELRK 617
Cdd:cd17642   472 GELPAAVVVL-----EAGKTMTEKEVMDYVASQVSTAKRLRgGVKFVDEVPKGLTGKIDRRKIRE 531
FADD10 cd17635
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ...
265-612 1.33e-38

adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.


Pssm-ID: 341290 [Multi-domain]  Cd Length: 340  Bit Score: 145.48  E-value: 1.33e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 265 EIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTHHlpRFE 344
Cdd:cd17635     2 DPLAVIFTSGTTGEPKAVLLANKTFFAVPDILQKEGLNWVVGDVTYLPLPATHIGGLWWILTCLIHGGLCVTGGE--NTT 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 345 PTSILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFKSLKHCVSAG--EPITPDVTEKWRNKTgLDIYEGYGQTET-VLI 421
Cdd:cd17635    80 YKSLFKILTTNAVTTTCLVPTLLSKLVSELKSANATVPSLRLIGYGgsRAIAADVRFIEATGL-TNTAQVYGLSETgTAL 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 422 CGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVlPNRPFGlfthYVDNPSKTASTLRGNFYITGDRGY 501
Cdd:cd17635   159 CLPTDDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKS-PANMLG----YWNNPERTAEVLIDGWVNTGDLGE 233
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 502 MDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNpdykSHDQEQLIKEIQE 581
Cdd:cd17635   234 RREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVAS----AELDENAIRALKH 309
                         330       340       350
                  ....*....|....*....|....*....|.
gi 1370468924 582 HVKKTTAPYKYPRKVEFIQELPKTISGKTKR 612
Cdd:cd17635   310 TIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
PLN02330 PLN02330
4-coumarate--CoA ligase-like 1
95-618 2.88e-38

4-coumarate--CoA ligase-like 1


Pssm-ID: 215189 [Multi-domain]  Cd Length: 546  Bit Score: 148.97  E-value: 2.88e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  95 WSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITN 174
Cdd:PLN02330   56 VTYGEVVRDTRRFAKALR-SLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFSGANPTALESEIKKQAEAAGAKLIVTN 134
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 175 DvlapavdAVASKCENLHSKLIV-SENSREGWGNLKELmkvflchpgwstvarswltatstslvqailslLSSWDyrHAS 253
Cdd:PLN02330  135 D-------TNYGKVKGLGLPVIVlGEEKIEGAVNWKEL--------------------------------LEAAD--RAG 173
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 254 DSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTH---------SSFGLGLSVNGRF-WLDLTPSDVMWNtsdtgwaksaws 323
Cdd:PLN02330  174 DTSDNEEILQTDLCALPFSSGTTGISKGVMLTHrnlvanlcsSLFSVGPEMIGQVvTLGLIPFFHIYG------------ 241
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 324 svfspwIQGACVFTHH-------LPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN------DITSYKFKSLkhcVSAG 390
Cdd:PLN02330  242 ------ITGICCATLRnkgkvvvMSRFELRTFLNALITQEVSFAPIVPPIILNLVKNpiveefDLSKLKLQAI---MTAA 312
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 391 EPITPDVTEKWRNK-TGLDIYEGYGQTETVLICGNF----KGMKI-KPGSMGKPSPAFDVKIVDV-NGNVLPPGQEGDIG 463
Cdd:PLN02330  313 APLAPELLTAFEAKfPGVQVQEAYGLTEHSCITLTHgdpeKGHGIaKKNSVGFILPNLEVKFIDPdTGRSLPKNTPGELC 392
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 464 IqvlpnRPFGLFTHYVDNPSKTASTLRGNFYI-TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAE 542
Cdd:PLN02330  393 V-----RSQCVMQGYYNNKEETDRTIDEDGWLhTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVED 467
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1370468924 543 SAVVSSPDPIRGEVVKAFVVLNPDYKSHDQeqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKK 618
Cdd:PLN02330  468 AAVVPLPDEEAGEIPAACVVINPKAKESEE-----DILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKEK 538
PRK06145 PRK06145
acyl-CoA synthetase; Validated
264-616 5.92e-38

acyl-CoA synthetase; Validated


Pssm-ID: 102207 [Multi-domain]  Cd Length: 497  Bit Score: 147.34  E-value: 5.92e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 264 NEIMAIFFTSGTSGYPKMTAHTHSSF---------GLGLSVNGRFW----------LDLTPSDVMWntsdtgwaksawss 324
Cdd:PRK06145  149 TDLVRLMYTSGTTDRPKGVMHSYGNLhwksidhviALGLTASERLLvvgplyhvgaFDLPGIAVLW-------------- 214
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 325 vfspwiQGACVFTHHlpRFEPTSILQTLSKYPITVFCSAPTVY-RMLVQNDITSYKFKSLKHCVSAGEPiTP-----DVT 398
Cdd:PRK06145  215 ------VGGTLRIHR--EFDPEAVLAAIERHRLTCAWMAPVMLsRVLTVPDRDRFDLDSLAWCIGGGEK-TPesrirDFT 285
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 399 EKWRNKTGLDiyeGYGQTETvliCGNFKGMKI-----KPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVlPNRPFG 473
Cdd:PRK06145  286 RVFTRARYID---AYGLTET---CSGDTLMEAgreieKIGSTGRALAHVEIRIADGAGRWLPPNMKGEICMRG-PKVTKG 358
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 474 lfthYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIR 553
Cdd:PRK06145  359 ----YWKDPEKTAEAFYGDWFRSGDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRW 434
                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1370468924 554 GEVVKAFVVLNPdykshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PRK06145  435 GERITAVVVLNP-----GATLTLEALDRHCRQRLASFKVPRQLKVRDELPRNPSGKVLKRVLR 492
A_NRPS_PvdJ-like cd17649
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ...
254-616 6.44e-36

non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341304 [Multi-domain]  Cd Length: 450  Bit Score: 140.58  E-value: 6.44e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 254 DS-HTCVKTKHNEIMA-IFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFwLDLTPSDVMWNTSDTGWaKSAWSSVFSPWIQ 331
Cdd:cd17649    82 DSgAGLLLTHHPRQLAyVIYTSGSTGTPKGVAVSHGPLAAHCQATAER-YGLTPGDRELQFASFNF-DGAHEQLLPPLIC 159
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 332 GACVFTHHLPRFEPTSILQTL-SKYPITVFCSAPTVYRMLVQ--NDITSYKFKSLKHCVSAGEPITPDVTEKWRnKTGLD 408
Cdd:cd17649   160 GACVVLRPDELWASADELAEMvRELGVTVLDLPPAYLQQLAEeaDRTGDGRPPSLRLYIFGGEALSPELLRRWL-KAPVR 238
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 409 IYEGYGQTETVLICGNFK---GMKIKPGSM--GKPSPAFDVKIVDVNGNVLPPGQEGD--IGIQVLP----NRPfGLfTH 477
Cdd:cd17649   239 LFNAYGPTEATVTPLVWKceaGAARAGASMpiGRPLGGRSAYILDADLNPVPVGVTGElyIGGEGLArgylGRP-EL-TA 316
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 478 --YVDNPSKTAStlrGNFYITGD--RgYMDkDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIR 553
Cdd:cd17649   317 erFVPDPFGAPG---SRLYRTGDlaR-WRD-DGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDGAGG 391
                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1370468924 554 GEVVkAFVVLNPDYKshdQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:cd17649   392 KQLV-AYVVLRAAAA---QPELRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
PRK08162 PRK08162
acyl-CoA synthetase; Validated
91-618 7.93e-36

acyl-CoA synthetase; Validated


Pssm-ID: 236169 [Multi-domain]  Cd Length: 545  Bit Score: 141.62  E-value: 7.93e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  91 EEMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANC 170
Cdd:PRK08162   40 GDRRRTWAETYARCRRLASALARR-GIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAASIAFMLRHGEAKV 118
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 171 IITNDVLAPAVDAVASKCENLHSKLI-VSENSREGWGNLKEL-MKVFLCH--PG--WSTVARSWltatstslvQAIlsll 244
Cdd:PRK08162  119 LIVDTEFAEVAREALALLPGPKPLVIdVDDPEYPGGRFIGALdYEAFLASgdPDfaWTLPADEW---------DAI---- 185
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 245 sswdyrhasdshtcvktkhneimAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWlDLTPSDV-MW-------NtsdtG 316
Cdd:PRK08162  186 -----------------------ALNYTSGTTGNPKGVVYHHRGAYLNALSNILAW-GMPKHPVyLWtlpmfhcN----G 237
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 317 WAksawssvFsPWIQGACVFTH-HLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVqNDITSYKfKSLKHCVS---AGEP 392
Cdd:PRK08162  238 WC-------F-PWTVAARAGTNvCLRKVDPKLIFDLIREHGVTHYCGAPIVLSALI-NAPAEWR-AGIDHPVHamvAGAA 307
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 393 ITPDVTEKWRNkTGLDIYEGYGQTET---VLIC--------------GNFKGMK------------IKPGSMgKPSPAFD 443
Cdd:PRK08162  308 PPAAVIAKMEE-IGFDLTHVYGLTETygpATVCawqpewdalplderAQLKARQgvryplqegvtvLDPDTM-QPVPADG 385
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 444 VKI--VDVNGNVLPPGqegdigiqvlpnrpfglfthYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSS 521
Cdd:PRK08162  386 ETIgeIMFRGNIVMKG--------------------YLKNPKATEEAFAGGWFHTGDLAVLHPDGYIKIKDRSKDIIISG 445
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 522 GYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyKSHDQEqlikEIQEHVKKTTAPYKYPRKVEFiQE 601
Cdd:PRK08162  446 GENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDG-ASATEE----EIIAHCREHLAGFKVPKAVVF-GE 519
                         570
                  ....*....|....*..
gi 1370468924 602 LPKTISGKTKRNELRKK 618
Cdd:PRK08162  520 LPKTSTGKIQKFVLREQ 536
OSB_MenE-like cd17630
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ...
269-618 1.48e-35

O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.


Pssm-ID: 341285 [Multi-domain]  Cd Length: 325  Bit Score: 136.31  E-value: 1.48e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPKMTAHT-----HSSFG----LGLSVNGRfWLDLTPS------DVMWNtsdtgWAKSAWSSVFSPWIQGA 333
Cdd:cd17630     5 VILTSGSTGTPKAVVHTaanllASAAGlhsrLGFGGGDS-WLLSLPLyhvgglAILVR-----SLLAGAELVLLERNQAL 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 334 cvfthhlprfeptsiLQTLSKYPITVFCSAPTVYRMLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKtGLDIYEGY 413
Cdd:cd17630    79 ---------------AEDLAPPGVTHVSLVPTQLQRLLDSGQGPAALKSLRAVLLGGAPIPPELLERAADR-GIPLYTTY 142
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 414 GQTETV-LICGNFKGMKiKPGSMGKPSPAFDVKIVDvngnvlppgqEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGN 492
Cdd:cd17630   143 GMTETAsQVATKRPDGF-GRGGVGVLLPGRELRIVE----------DGEIWV-----GGASLAMGYLRGQLVPEFNEDGW 206
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 493 FYiTGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPdykSHDQ 572
Cdd:cd17630   207 FT-TKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRG---PADP 282
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*.
gi 1370468924 573 EQLIkeiqEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKK 618
Cdd:cd17630   283 AELR----AWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAW 324
PRK06060 PRK06060
p-hydroxybenzoic acid--AMP ligase FadD22;
117-616 1.96e-35

p-hydroxybenzoic acid--AMP ligase FadD22;


Pssm-ID: 180374 [Multi-domain]  Cd Length: 705  Bit Score: 142.09  E-value: 1.96e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 117 LQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLapavdavaskCENLHSKLI 196
Cdd:PRK06060   52 LSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLANPELHRDDHALAARNTEPALVVTSDAL----------RDRFQPSRV 121
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 197 VSEnsregwgnlKELMkvflchpgwSTVARSwltatstslvqailsllSSWDYRHASDSHTCVKTkhneimaifFTSGTS 276
Cdd:PRK06060  122 AEA---------AELM---------SEAARV-----------------APGGYEPMGGDALAYAT---------YTSGTT 157
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 277 GYPKMTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTHHLP-RFEPTSILQTlsKY 355
Cdd:PRK06060  158 GPPKAAIHRHADPLTFVDAMCRKALRLTPEDTGLCSARMYFAYGLGNSVWFPLATGGSAVINSAPvTPEAAAILSA--RF 235
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 356 PITVFCSAPTVYRMLVqNDITSYKFKSLKHCVSAGEPITPDVTEKWRNK-TGLDIYEGYGQTEtvlICGNFKGMKI---K 431
Cdd:PRK06060  236 GPSVLYGVPNFFARVI-DSCSPDSFRSLRCVVSAGEALELGLAERLMEFfGGIPILDGIGSTE---VGQTFVSNRVdewR 311
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 432 PGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGI------QVLPNRPFGLFTHyvdnpsktastlrGNFYITGDRGYMDKD 505
Cdd:PRK06060  312 LGTLGRVLPPYEIRVVAPDGTTAGPGVEGDLWVrgpaiaKGYWNRPDSPVAN-------------EGWLDTRDRVCIDSD 378
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 506 GYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVlnPDYKSHDQEQLIKEIQEHVKK 585
Cdd:PRK06060  379 GWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLV--ATSGATIDGSVMRDLHRGLLN 456
                         490       500       510
                  ....*....|....*....|....*....|.
gi 1370468924 586 TTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PRK06060  457 RLSAFKVPHRFAVVDRLPRTPNGKLVRGALR 487
CBAL cd05923
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ...
337-615 2.38e-35

4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.


Pssm-ID: 341247 [Multi-domain]  Cd Length: 493  Bit Score: 139.57  E-value: 2.38e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 337 THHLPR-FEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYG 414
Cdd:cd05923   221 TYVVVEeFDPADALKLIEQERVTSLFATPTHLDALAAAaEFAGLKLSSLRHVTFAGATMPDAVLERVNQHLPGEKVNIYG 300
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 415 QTETVlicgNFKGMK-IKPGSMGKPSPAFDVKIVDVNGNV---LPPGQEGDIGIQVLPNRPFglfTHYVDNPSKTASTLR 490
Cdd:cd05923   301 TTEAM----NSLYMRdARTGTEMRPGFFSEVRIVRIGGSPdeaLANGEEGELIVAAAADAAF---TGYLNQPEATAKKLQ 373
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 491 GNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSH 570
Cdd:cd05923   374 DGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPREGTLSA 453
                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*.
gi 1370468924 571 DqeqlikEIQEHVKKTT-APYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd05923   454 D------ELDQFCRASElADFKRPRRYFFLDELPKNAMNKVLRRQL 493
A_NRPS_AB3403-like cd17646
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ...
72-615 3.17e-35

Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341301 [Multi-domain]  Cd Length: 488  Bit Score: 138.95  E-value: 3.17e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  72 EKAGKKPSNPAFWWinrngEEMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGT 151
Cdd:cd17646     6 EQAARTPDAPAVVD-----EGRTLTYRELDERANRLAHLLRAR-GVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLD 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 152 TQLTQKDILYRLQSSKANCIITNDVLA---PAVDAVASKCEnlhsklivsensregwgnlkelmkvflchpgwstvarsW 228
Cdd:cd17646    80 PGYPADRLAYMLADAGPAVVLTTADLAarlPAGGDVALLGD--------------------------------------E 121
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 229 LTATSTSLVQAILSLLSSWDYrhasdshtcvktkhneimaIFFTSGTSGYPKMTAHTHSSFglglsVNGRFWL----DLT 304
Cdd:cd17646   122 ALAAPPATPPLVPPRPDNLAY-------------------VIYTSGSTGRPKGVMVTHAGI-----VNRLLWMqdeyPLG 177
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 305 PSDVMWNTSDTGWAKSAWSsVFSPWIQGAC-VFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQnDITSYKFKSL 383
Cdd:cd17646   178 PGDRVLQKTPLSFDVSVWE-LFWPLVAGARlVVARPGGHRDPAYLAALIREHGVTTCHFVPSMLRVFLA-EPAAGSCASL 255
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 384 KHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTET---VLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEG 460
Cdd:cd17646   256 RRVFCSGEALPPELAARFLALPGAELHNLYGPTEAaidVTHWPVRGPAETPSVPIGRPVPNTRLYVLDDALRPVPVGVPG 335
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 461 DI---GIQV---LPNRPfGLfT--HYVDNPSKTASTLrgnfYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVEN 532
Cdd:cd17646   336 ELylgGVQLargYLGRP-AL-TaeRFVPDPFGPGSRM----YRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEA 409
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 533 ALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQEQLikeiQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKR 612
Cdd:cd17646   410 ALAAHPAVTHAVVVARAAPAGAARLVGYVVPAAGAAGPDTAAL----RAHLAERLPEYMVPAAFVVLDALPLTANGKLDR 485

                  ...
gi 1370468924 613 NEL 615
Cdd:cd17646   486 AAL 488
FAA1 COG1022
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
74-618 3.52e-35

Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];


Pssm-ID: 440645 [Multi-domain]  Cd Length: 603  Bit Score: 140.62  E-value: 3.52e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  74 AGKKPSNPAFWWiNRNGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQ 153
Cdd:COG1022    21 AARFPDRVALRE-KEDGIWQSLTWAEFAERVRALAAGL-LALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVPIYPT 98
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 154 LTQKDILYRLQSSKANCIIT-NDVLAPAVDAVASKCENLhsKLIVSENSREGWGNLKELmkvflchpGWSTVARSWLTAT 232
Cdd:COG1022    99 SSAEEVAYILNDSGAKVLFVeDQEQLDKLLEVRDELPSL--RHIVVLDPRGLRDDPRLL--------SLDELLALGREVA 168
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 233 STSLVQAILSllsswdyrhasdshtcvKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDV---- 308
Cdd:COG1022   169 DPAELEARRA-----------------AVKPDDLATIIYTSGTTGRPKGVMLTHRNL-LSNARALLERLPLGPGDRtlsf 230
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 309 --MWNTSDTGWaksawsSVFSpWIQGACVftHHLPRfePTSILQTLSKYPITVFCSAPTVYRMlVQNDITS--------- 377
Cdd:COG1022   231 lpLAHVFERTV------SYYA-LAAGATV--AFAES--PDTLAEDLREVKPTFMLAVPRVWEK-VYAGIQAkaeeagglk 298
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 378 ---------------------------YKFK------------------SLKHCVSAGEPITPDVTEKWRNkTGLDIYEG 412
Cdd:COG1022   299 rklfrwalavgrryararlagkspsllLRLKhaladklvfsklrealggRLRFAVSGGAALGPELARFFRA-LGIPVLEG 377
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 413 YGQTET-VLICGNFKGmKIKPGSMGKPSPAFDVKIvdvngnvlppGQEGDI---GIQVlpnrpfglFTHYVDNPSKTAST 488
Cdd:COG1022   378 YGLTETsPVITVNRPG-DNRIGTVGPPLPGVEVKI----------AEDGEIlvrGPNV--------MKGYYKNPEATAEA 438
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 489 LR--GNFYiTGDRGYMDKDGYFWFVARADDVI-LSSGYRIGPFEVENALNEHPSVAESAVVsspdpirGE----VVkAFV 561
Cdd:COG1022   439 FDadGWLH-TGDIGELDEDGFLRITGRKKDLIvTSGGKNVAPQPIENALKASPLIEQAVVV-------GDgrpfLA-ALI 509
                         570       580       590       600       610       620       630       640
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 562 VLNPD------------YKSHDQ----EQLIKEIQEHVKKTT---APYKYPRKVEFiqeLPK---------TISGKTKRN 613
Cdd:COG1022   510 VPDFEalgewaeenglpYTSYAElaqdPEVRALIQEEVDRANaglSRAEQIKRFRL---LPKeftiengelTPTLKLKRK 586

                  ....*
gi 1370468924 614 ELRKK 618
Cdd:COG1022   587 VILEK 591
PRK09088 PRK09088
acyl-CoA synthetase; Validated
269-616 4.29e-35

acyl-CoA synthetase; Validated


Pssm-ID: 181644 [Multi-domain]  Cd Length: 488  Bit Score: 138.79  E-value: 4.29e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPK--MTA-----HTHSSFGLGLSVNGR--FWLDlTPsdvMWNTsdTGWAksawSSVFSPWIQGACVFTHh 339
Cdd:PRK09088  140 ILFTSGTSGQPKgvMLSernlqQTAHNFGVLGRVDAHssFLCD-AP---MFHI--IGLI----TSVRPVLAVGGSILVS- 208
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 340 lPRFEPTSILQTLS--KYPITVFCSAPTVYRML-VQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKtGLDIYEGYGQT 416
Cdd:PRK09088  209 -NGFEPKRTLGRLGdpALGITHYFCVPQMAQAFrAQPGFDAAALRHLTALFTGGAPHAAEDILGWLDD-GIPMVDGFGMS 286
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 417 E--TVLicgnfkGMKI-------KPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVlPNrpfgLFTHYVDNPSKTAS 487
Cdd:PRK09088  287 EagTVF------GMSVdcdviraKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLLRG-PN----LSPGYWRRPQATAR 355
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 488 TLRGN-FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPd 566
Cdd:PRK09088  356 AFTGDgWFRTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPAD- 434
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|
gi 1370468924 567 ykshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PRK09088  435 ----GAPLDLERIRSHLSTRLAKYKVPKHLRLVDALPRTASGKLQKARLR 480
A_NRPS_VisG_like cd17651
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ...
90-616 1.45e-34

similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341306 [Multi-domain]  Cd Length: 491  Bit Score: 137.09  E-value: 1.45e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  90 GEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKAN 169
Cdd:cd17651    16 AEGRRLTYAELDRRANRLAHRL-RARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYPAERLAFMLADAGPV 94
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 170 CIITNDVLAPAVDAVAskcenlhsklivsensregwgnlkelmkvflcHPGWSTVARSWLTATSTSLVQAilsllsswdy 249
Cdd:cd17651    95 LVLTHPALAGELAVEL--------------------------------VAVTLLDQPGAAAGADAEPDPA---------- 132
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 250 RHASDshtcvktkhneIMAIFFTSGTSGYPKMTAHTHSSFglglsVNGRFWLD----LTPSDVMWNTSDTGWAKSAWSsV 325
Cdd:cd17651   133 LDADD-----------LAYVIYTSGSTGRPKGVVMPHRSL-----ANLVAWQArassLGPGARTLQFAGLGFDVSVQE-I 195
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 326 FSPWIQGACVfthHLP----RFEPTSILQTLSKYPITVfCSAPTVY--RMLVQNDITSYKFKSLKHCVSAGEP--ITPDV 397
Cdd:cd17651   196 FSTLCAGATL---VLPpeevRTDPPALAAWLDEQRISR-VFLPTVAlrALAEHGRPLGVRLAALRYLLTGGEQlvLTEDL 271
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 398 TEKWRNKTGLDIYEGYGQTE----TVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQVLP-- 468
Cdd:cd17651   272 REFCAGLPGLRLHNHYGPTEthvvTALSLPGDPAAWPAPPPIGRPIDNTRVYVLDAALRPVPPGVPGELyigGAGLARgy 351
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 469 -NRPfGLfTH--YVDNPSKTASTLrgnfYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAV 545
Cdd:cd17651   352 lNRP-EL-TAerFVPDPFVPGARM----YRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAVV 425
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1370468924 546 VSSPDPIRGEVVKAFVVLNPdykshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:cd17651   426 LAREDRPGEKRLVAYVVGDP-----EAPVDAAELRAALATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
PLN02574 PLN02574
4-coumarate--CoA ligase-like
253-616 1.19e-33

4-coumarate--CoA ligase-like


Pssm-ID: 215312 [Multi-domain]  Cd Length: 560  Bit Score: 135.74  E-value: 1.19e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 253 SDSHTCVK--TKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFwldltpsdvmwntSDTGWAKSAWSSVFSP-- 328
Cdd:PLN02574  185 EDFDFVPKpvIKQDDVAAIMYSSGTTGASKGVVLTHRNLIAMVELFVRF-------------EASQYEYPGSDNVYLAal 251
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 329 ---WIQGACVFTHHL----------PRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN--DITSYKFKSLKHCVSAGEPI 393
Cdd:PLN02574  252 pmfHIYGLSLFVVGLlslgstivvmRRFDASDMVKVIDRFKVTHFPVVPPILMALTKKakGVCGEVLKSLKQVSCGAAPL 331
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 394 TPDVTEKW-RNKTGLDIYEGYGQTETVLICG---NFKGMKiKPGSMGKPSPAFDVKIVD-VNGNVLPPGQEGDIGIQvlp 468
Cdd:PLN02574  332 SGKFIQDFvQTLPHVDFIQGYGMTESTAVGTrgfNTEKLS-KYSSVGLLAPNMQAKVVDwSTGCLLPPGNCGELWIQ--- 407
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 469 nRPfGLFTHYVDNPSKTASTLRGNFYI-TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVS 547
Cdd:PLN02574  408 -GP-GVMKGYLNNPKATQSTIDKDGWLrTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTA 485
                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1370468924 548 SPDPIRGEVVKAFVVLNPDyKSHDQEQLIkeiqEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PLN02574  486 VPDKECGEIPVAFVVRRQG-STLSQEAVI----NYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELK 549
EntE COG1021
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ...
63-617 3.46e-33

EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440644 [Multi-domain]  Cd Length: 533  Bit Score: 133.73  E-value: 3.46e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  63 DVLDQWtdkekAGKKPSNPAFwwInrnGEEMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLR 142
Cdd:COG1021    29 DLLRRR-----AERHPDRIAV--V---DGERRLSYAELDRRADRLAAGLLAL-GLRPGDRVVVQLPNVAEFVIVFFALFR 97
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 143 TGtvLIPGTT--QLTQKDILYRLQSSKANCIITNDV-----LAPAVDAVASKCENLhsKLIVSENSREGWGNLKELMKvf 215
Cdd:COG1021    98 AG--AIPVFAlpAHRRAEISHFAEQSEAVAYIIPDRhrgfdYRALARELQAEVPSL--RHVLVVGDAGEFTSLDALLA-- 171
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 216 lcHPGWSTVARSwltatstslvqailsllsswdyrHASDshtcvktkhneiMAIFFTS-GTSGYPKMTAHTHSSFGLGLS 294
Cdd:COG1021   172 --APADLSEPRP-----------------------DPDD------------VAFFQLSgGTTGLPKLIPRTHDDYLYSVR 214
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 295 VNGRFWlDLTPSDVMW-------NtsdtgwakSAWSS--VFSPWIQGACVFTHhlPRFEPTSILQTLSKYPITVFCSAPT 365
Cdd:COG1021   215 ASAEIC-GLDADTVYLaalpaahN--------FPLSSpgVLGVLYAGGTVVLA--PDPSPDTAFPLIERERVTVTALVPP 283
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 366 VYRMLVQ-NDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYG-------QT------ETVLicgnfkgmkik 431
Cdd:COG1021   284 LALLWLDaAERSRYDLSSLRVLQVGGAKLSPELARRVRPALGCTLQQVFGmaeglvnYTrlddpeEVIL----------- 352
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 432 pGSMGKP-SPAFDVKIVDVNGNVLPPGQEGdigiqVLPNRPFGLFTHYVDNPSKTAS--TLRGnFYITGDRGYMDKDGYF 508
Cdd:COG1021   353 -TTQGRPiSPDDEVRIVDEDGNPVPPGEVG-----ELLTRGPYTIRGYYRAPEHNARafTPDG-FYRTGDLVRRTPDGYL 425
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 509 WFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNpdykshDQEQLIKEIQEHVK-KTT 587
Cdd:COG1021   426 VVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPR------GEPLTLAELRRFLReRGL 499
                         570       580       590
                  ....*....|....*....|....*....|
gi 1370468924 588 APYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:COG1021   500 AAFKLPDRLEFVDALPLTAVGKIDKKALRA 529
A_NRPS_Srf_like cd12117
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ...
269-615 4.37e-33

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.


Pssm-ID: 341282 [Multi-domain]  Cd Length: 483  Bit Score: 132.71  E-value: 4.37e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPKMTAHTHSSFgLGLsVNGRFWLDLTPSDVMWNTSDTGWAKSAWSsVFSPWIQGACVF---THHLPRfeP 345
Cdd:cd12117   141 VMYTSGSTGRPKGVAVTHRGV-VRL-VKNTNYVTLGPDDRVLQTSPLAFDASTFE-IWGALLNGARLVlapKGTLLD--P 215
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 346 TSILQTLSKYPITV-FCSAPtVYRMLVQNDITSykFKSLKHCVSAGEPITPDVTEKWRNKT-GLDIYEGYGQTE--TVLI 421
Cdd:cd12117   216 DALGALIAEEGVTVlWLTAA-LFNQLADEDPEC--FAGLRELLTGGEVVSPPHVRRVLAACpGLRLVNGYGPTEntTFTT 292
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 422 CGNFKGMKIKPGS--MGKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQVLP---NRPFGLFTHYVDNPSKTASTLrgnf 493
Cdd:cd12117   293 SHVVTELDEVAGSipIGRPIANTRVYVLDEDGRPVPPGVPGELyvgGDGLALgylNRPALTAERFVADPFGPGERL---- 368
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 494 YITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAV-VSSPDPIRGEVVkAFVVLNPDyKSHDq 572
Cdd:cd12117   369 YRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVREAVVvVREDAGGDKRLV-AYVVAEGA-LDAA- 445
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|...
gi 1370468924 573 eqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd12117   446 -----ELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
AFD_YhfT-like cd17633
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ...
271-612 5.25e-33

fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain


Pssm-ID: 341288 [Multi-domain]  Cd Length: 320  Bit Score: 129.06  E-value: 5.25e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 271 FTSGTSGYPKMTAHTHSSfglglsvngrfWLDLTPSDVMwntsdtGWAKSAWSSVFSPwiqGACVFTHHL---------- 340
Cdd:cd17633     7 FTSGTTGLPKAYYRSERS-----------WIESFVCNED------LFNISGEDAILAP---GPLSHSLFLygaisalylg 66
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 341 ------PRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFKSLkhcVSAGEPITPDVTEKWRNKT-GLDIYEGY 413
Cdd:cd17633    67 gtfigqRKFNPKSWIRKINQYNATVIYLVPTMLQALARTLEPESKIKSI---FSSGQKLFESTKKKLKNIFpKANLIEFY 143
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 414 GQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVlppgqegdIG-IQVLPNRpfgLFTHYVDNPSKTAstlrGN 492
Cdd:cd17633   144 GTSELSFITYNFNQESRPPNSVGRPFPNVEIEIRNADGGE--------IGkIFVKSEM---VFSGYVRGGFSNP----DG 208
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 493 FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNpdyKSHDQ 572
Cdd:cd17633   209 WMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIAVALYSGD---KLTYK 285
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|
gi 1370468924 573 EQLIKeiqehVKKTTAPYKYPRKVEFIQELPKTISGKTKR 612
Cdd:cd17633   286 QLKRF-----LKQKLSRYEIPKKIIFVDSLPYTSSGKIAR 320
PRK08315 PRK08315
AMP-binding domain protein; Validated
94-618 5.76e-33

AMP-binding domain protein; Validated


Pssm-ID: 236236 [Multi-domain]  Cd Length: 559  Bit Score: 133.40  E-value: 5.76e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  94 RWSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSS--KANCI 171
Cdd:PRK08315   43 RWTYREFNEEVDALAKGLL-ALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAILVTINPAYRLSELEYALNQSgcKALIA 121
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 172 I----TNDVLApAVDAVASKCENLHSKLIVSENSREgwgnLKELmkVFLC---HPG---WSTVARSWLTATSTSLvQAIL 241
Cdd:PRK08315  122 AdgfkDSDYVA-MLYELAPELATCEPGQLQSARLPE----LRRV--IFLGdekHPGmlnFDELLALGRAVDDAEL-AARQ 193
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 242 SLLSSWDyrhasdshtcvktkhneIMAIFFTSGTSGYPKMTAHTHSSFGLglsvNGRF---WLDLTPSD----------- 307
Cdd:PRK08315  194 ATLDPDD-----------------PINIQYTSGTTGFPKGATLTHRNILN----NGYFigeAMKLTEEDrlcipvplyhc 252
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 308 ---VMWNTsdtgwaksawssvfspwiqgACVfTHH------LPRFEPTSILQTLSKYPITVFCSAPTVY-RMLVQNDITS 377
Cdd:PRK08315  253 fgmVLGNL--------------------ACV-THGatmvypGEGFDPLATLAAVEEERCTALYGVPTMFiAELDHPDFAR 311
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 378 YKFKSLKHCVSAGEPITPDVTEKWRNKTGL-DIYEGYGQTETvlicgnfkgmkiKPGSM---------------GKPSPA 441
Cdd:PRK08315  312 FDLSSLRTGIMAGSPCPIEVMKRVIDKMHMsEVTIAYGMTET------------SPVSTqtrtddplekrvttvGRALPH 379
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 442 FDVKIVD-VNGNVLPPGQEGDigiqvLPNRPFGLFTHYVDNPSKTASTL-RGNFYITGDRGYMDKDGYFWFVARADDVIL 519
Cdd:PRK08315  380 LEVKIVDpETGETVPRGEQGE-----LCTRGYSVMKGYWNDPEKTAEAIdADGWMHTGDLAVMDEEGYVNIVGRIKDMII 454
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 520 SSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQeqlikEIQEHVKKTTAPYKYPRKVEFI 599
Cdd:PRK08315  455 RGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTEE-----DVRDFCRGKIAHYKIPRYIRFV 529
                         570
                  ....*....|....*....
gi 1370468924 600 QELPKTISGKTKRNELRKK 618
Cdd:PRK08315  530 DEFPMTVTGKIQKFKMREM 548
A_NRPS_Cytc1-like cd17643
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ...
269-615 2.01e-32

similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341298 [Multi-domain]  Cd Length: 450  Bit Score: 130.51  E-value: 2.01e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSsVFSPWIQGA--CVFTHHLPRfEPT 346
Cdd:cd17643    98 VIYTSGSTGRPKGVVVSHANV-LALFAATQRWFGFNEDDVWTLFHSYAFDFSVWE-IWGALLHGGrlVVVPYEVAR-SPE 174
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 347 SILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFK-SLKHCVSAGEPITPDVTEKWRNKTGL---DIYEGYGQTET-VLI 421
Cdd:cd17643   175 DFARLLRDEGVTVLNQTPSAFYQLVEAADRDGRDPlALRYVIFGGEALEAAMLRPWAGRFGLdrpQLVNMYGITETtVHV 254
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 422 cgNFKGMK---IKPGSM---GKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQVLP---NRPFGLFTHYVDNPsKTASTL 489
Cdd:cd17643   255 --TFRPLDaadLPAAAAspiGRPLPGLRVYVLDADGRPVPPGVVGELyvsGAGVARgylGRPELTAERFVANP-FGGPGS 331
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 490 RGnfYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNpdyks 569
Cdd:cd17643   332 RM--YRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDAAVIVREDEPGDTRLVAYVVAD----- 404
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*.
gi 1370468924 570 HDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd17643   405 DGAAADIAELRALLKELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
VL_LC_FACS_like cd05907
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ...
95-615 3.08e-32

Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341233 [Multi-domain]  Cd Length: 452  Bit Score: 129.64  E-value: 3.08e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  95 WSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKAnciitn 174
Cdd:cd05907     6 ITWAEFAEEVRALAKGL-IALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEA------ 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 175 dvlapavdavaskcenlhsKLIVSENSREgwgnlkelmkvflchpgwstvarswlTATstslvqailsllsswdyrhasd 254
Cdd:cd05907    79 -------------------KALFVEDPDD--------------------------LAT---------------------- 91
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 255 shtcvktkhneimaIFFTSGTSGYPKMTAHTHSSFglglsvngrfwldltpsdvMWN--TSDTGWAKSAWSSVFS----- 327
Cdd:cd05907    92 --------------IIYTSGTTGRPKGVMLSHRNI-------------------LSNalALAERLPATEGDRHLSflpla 138
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 328 -----------PWIQGACVFthHLPRFEptSILQTLSKYPITVFCSAPTVYRML----VQNDITSYK--------FKSLK 384
Cdd:cd05907   139 hvferraglyvPLLAGARIY--FASSAE--TLLDDLSEVRPTVFLAVPRVWEKVyaaiKVKAVPGLKrklfdlavGGRLR 214
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 385 HCVSAGEPITPDVTEKWRnKTGLDIYEGYGQTETV-LICGNFKGmKIKPGSMGKPSPAFDVKIVDVNgnvlppgqEgdig 463
Cdd:cd05907   215 FAASGGAPLPAELLHFFR-ALGIPVYEGYGLTETSaVVTLNPPG-DNRIGTVGKPLPGVEVRIADDG--------E---- 280
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 464 IQVlpnRPFGLFTHYVDNPSKTA-STLRGNFYITGDRGYMDKDGYFWFVARADDVI-LSSGYRIGPFEVENALNEHPSVA 541
Cdd:cd05907   281 ILV---RGPNVMLGYYKNPEATAeALDADGWLHTGDLGEIDEDGFLHITGRKKDLIiTSGGKNISPEPIENALKASPLIS 357
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 542 ESAVVSSPDPirgeVVKAFVVLNPDY-----KSHD-----------QEQLIKEIQEHVK---KTTAPYKYPRKVEFIQEl 602
Cdd:cd05907   358 QAVVIGDGRP----FLVALIVPDPEAleawaEEHGiaytdvaelaaNPAVRAEIEAAVEaanARLSRYEQIKKFLLLPE- 432
                         570       580
                  ....*....|....*....|
gi 1370468924 603 PKTI-------SGKTKRNEL 615
Cdd:cd05907   433 PFTIengeltpTLKLKRPVI 452
PTZ00237 PTZ00237
acetyl-CoA synthetase; Provisional
269-617 1.24e-31

acetyl-CoA synthetase; Provisional


Pssm-ID: 240325 [Multi-domain]  Cd Length: 647  Bit Score: 130.25  E-value: 1.24e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTGWAKsawssvFSPWIQGACVFTHHLPRFE---- 344
Cdd:PTZ00237  259 ILYTSGTTGNSKAVVRSNGPHLVGLKYYWRSIIEKDIPTVVFSHSSIGWVS------FHGFLYGSLSLGNTFVMFEggii 332
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 345 -----PTSILQTLSKYPITVFCSAPTVYRMLVQND-----ITS-YKFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGY 413
Cdd:PTZ00237  333 knkhiEDDLWNTIEKHKVTHTLTLPKTIRYLIKTDpeatiIRSkYDLSNLKEIWCGGEVIEESIPEYIENKLKIKSSRGY 412
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 414 GQTET-VLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvLPNRPFGLFTHYV-DNPSKTASTLRG 491
Cdd:PTZ00237  413 GQTEIgITYLYCYGHINIPYNATGVPSIFIKPSILSEDGKELNVNEIGEVAFK-LPMPPSFATTFYKnDEKFKQLFSKFP 491
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 492 NFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSH- 570
Cdd:PTZ00237  492 GYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECCSIGIYDPDCYNVPIGLLVLKQDQSNQs 571
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*...
gi 1370468924 571 -DQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PTZ00237  572 iDLNKLKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPRQIISK 619
PLN02860 PLN02860
o-succinylbenzoate-CoA ligase
212-617 6.34e-31

o-succinylbenzoate-CoA ligase


Pssm-ID: 215464 [Multi-domain]  Cd Length: 563  Bit Score: 127.61  E-value: 6.34e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 212 MKVFLCHPGWSTV--ARSWLTaTSTSLVQAILSllSSWDYRHASDShtcvktkhneIMAIFFTSGTSGYPKMTAHTHSSF 289
Cdd:PLN02860  131 WQVFLESPSSSVFifLNSFLT-TEMLKQRALGT--TELDYAWAPDD----------AVLICFTSGTTGRPKGVTISHSAL 197
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 290 ---GLG-LSVNGrfwldLTPSDVMWNTS---DTGwaksAWSSVFSPWIQGAC-VFthhLPRFEPTSILQTLSKYPITVFC 361
Cdd:PLN02860  198 ivqSLAkIAIVG-----YGEDDVYLHTAplcHIG----GLSSALAMLMVGAChVL---LPKFDAKAALQAIKQHNVTSMI 265
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 362 SAPTVYRMLV---QNDITSYKFKSLKHCVSAGEPIT----PDVTEKWRNKtglDIYEGYGQTET----------VLICGN 424
Cdd:PLN02860  266 TVPAMMADLIsltRKSMTWKVFPSVRKILNGGGSLSsrllPDAKKLFPNA---KLFSAYGMTEAcssltfmtlhDPTLES 342
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 425 FK-----GMKIKPGS--------MGKPSPAFDVKIvdvngnvlppGQEGDIGIQVLPNRPFGLFTHYVDNPSKTASTLRG 491
Cdd:PLN02860  343 PKqtlqtVNQTKSSSvhqpqgvcVGKPAPHVELKI----------GLDESSRVGRILTRGPHVMLGYWGQNSETASVLSN 412
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 492 NFYI-TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYK-S 569
Cdd:PLN02860  413 DGWLdTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVPDSRLTEMVVACVRLRDGWIwS 492
                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1370468924 570 HDQEQLIK--------EIQEHV-KKTTAPYKYPRK-VEFIQELPKTISGKTKRNELRK 617
Cdd:PLN02860  493 DNEKENAKknltlsseTLRHHCrEKNLSRFKIPKLfVQWRKPFPLTTTGKIRRDEVRR 550
A_NRPS_Bac cd17655
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ...
269-615 6.37e-31

bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341310 [Multi-domain]  Cd Length: 490  Bit Score: 126.67  E-value: 6.37e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPKMTAHTHSSFglglsVNGRFWLD----LTPSDVMWNTSDTGWAKSAWSsVFSPWIQGACVFTH-HLPRF 343
Cdd:cd17655   142 VIYTSGSTGKPKGVMIEHRGV-----VNLVEWANkviyQGEHLRVALFASISFDASVTE-IFASLLSGNTLYIVrKETVL 215
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 344 EPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSykFKSLKHCVSAGEPITPDVTEKW--RNKTGLDIYEGYGQTETVLI 421
Cdd:cd17655   216 DGQALTQYIRQNRITIIDLTPAHLKLLDAADDSE--GLSLKHLIVGGEALSTELAKKIieLFGTNPTITNAYGPTETTVD 293
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 422 C--GNFKGMKIKPGS--MGKPSPAFDVKIVDVNGNVLPPGQEGDIGI------QVLPNRPFGLFTHYVDNPSKTAstlrG 491
Cdd:cd17655   294 AsiYQYEPETDQQVSvpIGKPLGNTRIYILDQYGRPQPVGVAGELYIggegvaRGYLNRPELTAEKFVDDPFVPG----E 369
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 492 NFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKshd 571
Cdd:cd17655   370 RMYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIVSEKELP--- 446
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*.
gi 1370468924 572 qeqlIKEIQEHVKKTTAPYKYPRKveFIQ--ELPKTISGKTKRNEL 615
Cdd:cd17655   447 ----VAQLREFLARELPDYMIPSY--FIKldEIPLTPNGKVDRKAL 486
PLN02479 PLN02479
acetate-CoA ligase
267-618 1.55e-30

acetate-CoA ligase


Pssm-ID: 178097 [Multi-domain]  Cd Length: 567  Bit Score: 126.50  E-value: 1.55e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 267 MAIFFTSGTSGYPKmtahthssfGLGLSVNGRFWLDLTpSDVMWNTSD-------------TGWAKSaWSsvfspwIQGA 333
Cdd:PLN02479  198 IALGYTSGTTASPK---------GVVLHHRGAYLMALS-NALIWGMNEgavylwtlpmfhcNGWCFT-WT------LAAL 260
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 334 CVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVqNDITSYKFKSLKHCV---SAGEPITPDVTEKWrNKTGLDIY 410
Cdd:PLN02479  261 CGTNICLRQVTAKAIYSAIANYGVTHFCAAPVVLNTIV-NAPKSETILPLPRVVhvmTAGAAPPPSVLFAM-SEKGFRVT 338
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 411 EGYGQTETVlicgnfkgmkiKPGSMGKPSPAFD-----------------------VKIVDVNGNVLPPGQEGDIGIQVL 467
Cdd:PLN02479  339 HTYGLSETY-----------GPSTVCAWKPEWDslppeeqarlnarqgvryiglegLDVVDTKTMKPVPADGKTMGEIVM 407
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 468 pnRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVS 547
Cdd:PLN02479  408 --RGNMVMKGYLKNPKANEEAFANGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVA 485
                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1370468924 548 SPDPIRGEVVKAFVVLNPDYKSHDQEQLIKEIQEHVKKTTAPYKYPRKVEFiQELPKTISGKTKRNELRKK 618
Cdd:PLN02479  486 RPDERWGESPCAFVTLKPGVDKSDEAALAEDIMKFCRERLPAYWVPKSVVF-GPLPKTATGKIQKHVLRAK 555
PLN03102 PLN03102
acyl-activating enzyme; Provisional
105-616 1.99e-30

acyl-activating enzyme; Provisional


Pssm-ID: 215576 [Multi-domain]  Cd Length: 579  Bit Score: 126.29  E-value: 1.99e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 105 RKFANILSeaCSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAV 184
Cdd:PLN03102   51 RLAASLIS--LNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFVDRSFEPLAREV 128
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 185 ----ASKCENLHSKLI-VSENSREGWGNLKELMKVFLCHPGWSTvarswltatsTSLVQAILsllsswdyrhasdshtCV 259
Cdd:PLN03102  129 lhllSSEDSNLNLPVIfIHEIDFPKRPSSEELDYECLIQRGEPT----------PSLVARMF----------------RI 182
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 260 KTKHNEImAIFFTSGTSGYPKMTAHTHSSFGL-GLSVNGRFWLDLTPSdVMWNTSD---TGWAKSaWSSVFSpwiQGACV 335
Cdd:PLN03102  183 QDEHDPI-SLNYTSGTTADPKGVVISHRGAYLsTLSAIIGWEMGTCPV-YLWTLPMfhcNGWTFT-WGTAAR---GGTSV 256
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 336 FTHHLPRFEptsILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFKSLK-HCVSAGEPiTPDVTEKWRNKTGLDIYEGYG 414
Cdd:PLN03102  257 CMRHVTAPE---IYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRSGPvHVLTGGSP-PPAALVKKVQRLGFQVMHAYG 332
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 415 QTET---VLIC-----------GNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQE-GDIGIqvlpnRPFGLFTHYV 479
Cdd:PLN03102  333 LTEAtgpVLFCewqdewnrlpeNQQMELKARQGVSILGLADVDVKNKETQESVPRDGKTmGEIVI-----KGSSIMKGYL 407
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 480 DNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKA 559
Cdd:PLN03102  408 KNPKATSEAFKHGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPCA 487
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1370468924 560 FVVL--NPDYKSHDQEQLI---KEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PLN03102  488 FVVLekGETTKEDRVDKLVtreRDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLR 549
PRK07638 PRK07638
acyl-CoA synthetase; Validated
271-617 1.80e-29

acyl-CoA synthetase; Validated


Pssm-ID: 236071 [Multi-domain]  Cd Length: 487  Bit Score: 122.20  E-value: 1.80e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 271 FTSGTSGYPKMTAHTHSSfglglsvngrfWL---DLTPSDV-MWNTSDTGWAKSAWSSVF-----SPWIQGACVftHHLP 341
Cdd:PRK07638  150 FTSGSTGKPKAFLRAQQS-----------WLhsfDCNVHDFhMKREDSVLIAGTLVHSLFlygaiSTLYVGQTV--HLMR 216
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 342 RFEPTSILQTLSKYPITVFCSAPTVYRMLVQndITSYKFKSLKhCVSAGEPITPDVTEKWRNK-TGLDIYEGYGQTE--- 417
Cdd:PRK07638  217 KFIPNQVLDKLETENISVMYTVPTMLESLYK--ENRVIENKMK-IISSGAKWEAEAKEKIKNIfPYAKLYEFYGASElsf 293
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 418 -TVLICGNFKgmkIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIqvlpNRPFgLFTHYVdNPSKTASTLRGNFYIT 496
Cdd:PRK07638  294 vTALVDEESE---RRPNSVGRPFHNVQVRICNEAGEEVQKGEIGTVYV----KSPQ-FFMGYI-IGGVLARELNADGWMT 364
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 497 -GDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVvlnpdykshDQEQL 575
Cdd:PRK07638  365 vRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAII---------KGSAT 435
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|..
gi 1370468924 576 IKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK07638  436 KQQLKSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKS 477
LC_FACL_like cd05914
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ...
261-612 2.83e-29

Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341240 [Multi-domain]  Cd Length: 463  Bit Score: 121.40  E-value: 2.83e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 261 TKHNEIMAIFFTSGTSGYPKMTAHTHSSfgLGLSVNGRFWLD-LTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVftHH 339
Cdd:cd05914    86 SDEDDVALINYTSGTTGNSKGVMLTYRN--IVSNVDGVKEVVlLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHV--VF 161
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 340 LPRFePTSILQTLSKYPITVFCSAPTVYRM--------LVQNDITSYKFK------------------------SLKHCV 387
Cdd:cd05914   162 LDKI-PSAKIIALAFAQVTPTLGVPVPLVIekifkmdiIPKLTLKKFKFKlakkinnrkirklafkkvheafggNIKEFV 240
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 388 SAGEPITPDVTEKWRnKTGLDIYEGYGQTETV-LICGNFKGmKIKPGSMGKPSPAFDVKIVDVNgnvlPPGQEGDIgiQV 466
Cdd:cd05914   241 IGGAKINPDVEEFLR-TIGFPYTIGYGMTETApIISYSPPN-RIRLGSAGKVIDGVEVRIDSPD----PATGEGEI--IV 312
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 467 lpnRPFGLFTHYVDNPSKTAS--TLRGNFYiTGDRGYMDKDGYFWFVARADDVI-LSSGYRIGPFEVENALNEHPSVAES 543
Cdd:cd05914   313 ---RGPNVMKGYYKNPEATAEafDKDGWFH-TGDLGKIDAEGYLYIRGRKKEMIvLSSGKNIYPEEIEAKINNMPFVLES 388
                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1370468924 544 AVVsspdpIRGEVVKAFVVLNPDY---KSHDQEQLIKEIQEHVKK----TTAPYKYPRKVEFI-QELPKTISGKTKR 612
Cdd:cd05914   389 LVV-----VQEKKLVALAYIDPDFldvKALKQRNIIDAIKWEVRDkvnqKVPNYKKISKVKIVkEEFEKTPKGKIKR 460
PRK06164 PRK06164
acyl-CoA synthetase; Validated
96-616 3.02e-29

acyl-CoA synthetase; Validated


Pssm-ID: 235722 [Multi-domain]  Cd Length: 540  Bit Score: 122.16  E-value: 3.02e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIitnd 175
Cdd:PRK06164   37 SRAELRALVDRLAAWLAAQ-GVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRYRSHEVAHILGRGRARWL---- 111
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 VLAPA---VDAVAskcenlhsklIVSENSREGwgnLKELMKVFLCHPGWSTVARSWLTATSTSLVQAILSLLSSWDYRHA 252
Cdd:PRK06164  112 VVWPGfkgIDFAA----------ILAAVPPDA---LPPLRAIAVVDDAADATPAPAPGARVQLFALPDPAPPAAAGERAA 178
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 253 SDSHTCVktkhneimaIFFTSGTSGYPKMTAHTHSSF---------GLGLSVNGRFWLDLTPSDVMwntsdtgwaksAWS 323
Cdd:PRK06164  179 DPDAGAL---------LFTTSGTTSGPKLVLHRQATLlrharaiarAYGYDPGAVLLAALPFCGVF-----------GFS 238
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 324 SVFSPWIQGACVftHHLPRFEPTSILQTLSKYPIT-VFCSAPTVYRMLVQNDiTSYKFKSLKHC-VSAGEPITPDVTEkW 401
Cdd:PRK06164  239 TLLGALAGGAPL--VCEPVFDAARTARALRRHRVThTFGNDEMLRRILDTAG-ERADFPSARLFgFASFAPALGELAA-L 314
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 402 RNKTGLDIYEGYGQTE--TVLICGNFK---GMKIKPGsmGKP-SPAFDVKIVDV-NGNVLPPGQEGDIGIQVlPNrpfgL 474
Cdd:PRK06164  315 ARARGVPLTGLYGSSEvqALVALQPATdpvSVRIEGG--GRPaSPEARVRARDPqDGALLPDGESGEIEIRA-PS----L 387
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 475 FTHYVDNPSKTASTLRGN-FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSpdPIR 553
Cdd:PRK06164  388 MRGYLDNPDATARALTDDgYFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGA--TRD 465
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1370468924 554 GE-VVKAFVVLNpDYKSHDQEQLIKeiqeHVKKTTAPYKYPRKVEFIQELPKTISG---KTKRNELR 616
Cdd:PRK06164  466 GKtVPVAFVIPT-DGASPDEAGLMA----ACREALAGFKVPARVQVVEAFPVTESAngaKIQKHRLR 527
PRK07798 PRK07798
acyl-CoA synthetase; Validated
58-609 6.85e-29

acyl-CoA synthetase; Validated


Pssm-ID: 236100 [Multi-domain]  Cd Length: 533  Bit Score: 121.15  E-value: 6.85e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  58 FNFAkDVLDQWTDKekagkKPSNPAFWWINRngeemRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLAN 137
Cdd:PRK07798    3 WNIA-DLFEAVADA-----VPDRVALVCGDR-----RLTYAELEERANRLAHYLIAQ-GLGPGDHVGIYARNRIEYVEAM 70
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 138 VACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIVSENSREGWGnlkelmkvflc 217
Cdd:PRK07798   71 LGAFKARAVPVNVNYRYVEDELRYLLDDSDAVALVYEREFAPRVAEVLPRLPKLRTLVVVEDGSGNDLL----------- 139
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 218 hPGwstvARSWLTATStslvqailsllsswdyrhASDSHTCVKTKHNEIMAIFFTSGTSGYPK--MTAHT---HSSFGLG 292
Cdd:PRK07798  140 -PG----AVDYEDALA------------------AGSPERDFGERSPDDLYLLYTGGTTGMPKgvMWRQEdifRVLLGGR 196
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 293 LSVNGRF------------------WLDLTPsdVMWNTSDtgWAksAWSSVFSpwiqGACVFTHHLPRFEPTSILQTLSK 354
Cdd:PRK07798  197 DFATGEPiedeeelakraaagpgmrRFPAPP--LMHGAGQ--WA--AFAALFS----GQTVVLLPDVRFDADEVWRTIER 266
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 355 YPITV-FCSAPTVYRMLVQ--NDITSYKFKSLKHCVSAGEPITPDVTEKWR----NKTGLDiyeGYGQTETvlicgNFKG 427
Cdd:PRK07798  267 EKVNViTIVGDAMARPLLDalEARGPYDLSSLFAIASGGALFSPSVKEALLellpNVVLTD---SIGSSET-----GFGG 338
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 428 MKI-KPGSMGKPSPAF----DVKIVDVNGNVLPPGqEGDIG-IQVLPNRPFGlfthYVDNPSKTASTLR---GNFY-ITG 497
Cdd:PRK07798  339 SGTvAKGAVHTGGPRFtigpRTVVLDEDGNPVEPG-SGEIGwIARRGHIPLG----YYKDPEKTAETFPtidGVRYaIPG 413
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 498 DRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDyKSHDQEqlik 577
Cdd:PRK07798  414 DRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAHPDVADALVVGVPDERWGQEVVAVVQLREG-ARPDLA---- 488
                         570       580       590
                  ....*....|....*....|....*....|..
gi 1370468924 578 EIQEHVKKTTAPYKYPRKVEFIQELPKTISGK 609
Cdd:PRK07798  489 ELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
AMP-binding_C pfam13193
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ...
529-609 1.15e-28

AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.


Pssm-ID: 463804 [Multi-domain]  Cd Length: 76  Bit Score: 108.79  E-value: 1.15e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 529 EVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYkshdqEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISG 608
Cdd:pfam13193   1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGV-----ELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSG 75

                  .
gi 1370468924 609 K 609
Cdd:pfam13193  76 K 76
23DHB-AMP_lg cd05920
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ...
90-615 1.37e-28

2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.


Pssm-ID: 341244 [Multi-domain]  Cd Length: 482  Bit Score: 119.35  E-value: 1.37e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  90 GEEMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKAN 169
Cdd:cd05920    36 DGDRRLTYRELDRRADRLAAGLRGL-GIRPGDRVVVQLPNVAEFVVLFFALLRLGAVPVLALPSHRRSELSAFCAHAEAV 114
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 170 CIITNDVLAPavdavaskcenlhsklivsensregwgnlkelmkvflchpgwstvarswltatstslvqailsllsswdY 249
Cdd:cd05920   115 AYIVPDRHAG---------------------------------------------------------------------F 125
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 250 RHASDSHTcVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSvngrfwldlTPSDVMWNTSDT----------GWAK 319
Cdd:cd05920   126 DHRALARE-LAESIPEVALFLLSGGTTGTPKLIPRTHNDYAYNVR---------ASAEVCGLDQDTvylavlpaahNFPL 195
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 320 SAWSSVFSPWIQGACVFThhlPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITS-YKFKSLKHCVSAGEPITPDVT 398
Cdd:cd05920   196 ACPGVLGTLLAGGRVVLA---PDPSPDAAFPLIEREGVTVTALVPALVSLWLDAAASRrADLSSLRLLQVGGARLSPALA 272
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 399 EKWRNKTGLDIYEGYGQTETVLicgNFKGM----KIKPGSMGKP-SPAFDVKIVDVNGNVLPPGQEGdigiQVLPNRPFg 473
Cdd:cd05920   273 RRVPPVLGCTLQQVFGMAEGLL---NYTRLddpdEVIIHTQGRPmSPDDEIRVVDEEGNPVPPGEEG----ELLTRGPY- 344
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 474 LFTHYVDNPSKTASTLRGN-FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPI 552
Cdd:cd05920   345 TIRGYYRAPEHNARAFTPDgFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDEL 424
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1370468924 553 RGEVVKAFVVLNpdykshDQEQLIKEIQEHVKKT-TAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd05920   425 LGERSCAFVVLR------DPPPSAAQLRRFLRERgLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
A_NRPS_Ta1_like cd12116
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ...
91-615 7.75e-28

The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.


Pssm-ID: 341281 [Multi-domain]  Cd Length: 470  Bit Score: 117.01  E-value: 7.75e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  91 EEMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANC 170
Cdd:cd12116     9 DDRSLSYAELDERANRLAARLRAR-GVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYILEDAEPAL 87
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 171 IITNDVLapavdavaskCENLHSKLIVSENSREGWGNlkelmkvflchpgwsTVARSWLTATSTSLVqailsllsswdYr 250
Cdd:cd12116    88 VLTDDAL----------PDRLPAGLPVLLLALAAAAA---------------APAAPRTPVSPDDLA-----------Y- 130
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 251 hasdshtcvktkhneimaIFFTSGTSGYPKMTAHTHSSF-GLGLSVNGRfwLDLTPSDVMWNTSDTGWAKSAWSsVFSPW 329
Cdd:cd12116   131 ------------------VIYTSGSTGRPKGVVVSHRNLvNFLHSMRER--LGLGPGDRLLAVTTYAFDISLLE-LLLPL 189
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 330 IQGACVftHHLPR---FEPTSILQTLSKYPITVFCSAPTVYRMLVQNDitsykFKSLKH----CvsAGEPITPDVTEKWR 402
Cdd:cd12116   190 LAGARV--VIAPRetqRDPEALARLIEAHSITVMQATPATWRMLLDAG-----WQGRAGltalC--GGEALPPDLAARLL 260
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 403 NKTGlDIYEGYGQTETVL------ICGNFKGMKIkpgsmGKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQVLP---NR 470
Cdd:cd12116   261 SRVG-SLWNLYGPTETTIwstaarVTAAAGPIPI-----GRPLANTQVYVLDAALRPVPPGVPGELyigGDGVAQgylGR 334
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 471 PFGLFTHYVDNPSKTAstlRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPD 550
Cdd:cd12116   335 PALTAERFVPDPFAGP---GSRLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVVRED 411
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1370468924 551 PIRGEVVkAFVVLnPDYKSHDqeqlIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd12116   412 GGDRRLV-AYVVL-KAGAAPD----AAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
A_NRPS_GliP_like cd17653
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ...
233-617 1.05e-27

nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341308 [Multi-domain]  Cd Length: 433  Bit Score: 116.25  E-value: 1.05e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 233 STSLVQAILSLL-----------SSWDYR-----HASDSHTCVKTKH-NEIMAIFFTSGTSGYPKMTAHTHSSFgLGLSV 295
Cdd:cd17653    57 SLEMLVAILAILkagaayvpldaKLPSARiqailRTSGATLLLTTDSpDDLAYIIFTSGSTGIPKGVMVPHRGV-LNYVS 135
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 296 NGRFWLDLTPSDVMWNTSDTGWAKSAWSsVFSPWIQGAcvfthHLPRFEPTSILQTLSKyPITVFCSAPTVYRMLVQNDi 375
Cdd:cd17653   136 QPPARLDVGPGSRVAQVLSIAFDACIGE-IFSTLCNGG-----TLVLADPSDPFAHVAR-TVDALMSTPSILSTLSPQD- 207
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 376 tsykFKSLKHCVSAGEPITPDVTEKWRNktGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLP 455
Cdd:cd17653   208 ----FPNLKTIFLGGEAVPPSLLDRWSP--GRRLYNAYGPTECTISSTMTELLPGQPVTIGKPIPNSTCYILDADLQPVP 281
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 456 PGQEGDI---GIQVLPNrpfglfthYVDNPSKTASTLRGN-------FYITGDRGYMDKDGYFWFVARADDVILSSGYRI 525
Cdd:cd17653   282 EGVVGEIcisGVQVARG--------YLGNPALTASKFVPDpfwpgsrMYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRI 353
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 526 GPFEVENALNEHPSVAESAVVSSpdpIRGEVVkAFVVlnPDykSHDQEQLIKEIQEHVkkttAPYKYPRKVEFIQELPKT 605
Cdd:cd17653   354 NLEEIEEVVLQSQPEVTQAAAIV---VNGRLV-AFVT--PE--TVDVDGLRSELAKHL----PSYAVPDRIIALDSFPLT 421
                         410
                  ....*....|..
gi 1370468924 606 ISGKTKRNELRK 617
Cdd:cd17653   422 ANGKVDRKALRE 433
PRK08633 PRK08633
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
258-610 1.61e-27

2-acyl-glycerophospho-ethanolamine acyltransferase; Validated


Pssm-ID: 236315 [Multi-domain]  Cd Length: 1146  Bit Score: 118.49  E-value: 1.61e-27
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  258 CVKTKHNE-IMAIFFTSGTSGYPK--MTAHT----------------------------HSsfgLGLSVNgrFWLdltps 306
Cdd:PRK08633   775 YGPTFKPDdTATIIFSSGSEGEPKgvMLSHHnilsnieqisdvfnlrnddvilsslpffHS---FGLTVT--LWL----- 844
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  307 dvmwntsdtgwaksawssvfsPWIQGACVFTHHLPrFEPTSILQTLSKYPITVFCSAPTVYRMLVQND-ITSYKFKSLKH 385
Cdd:PRK08633   845 ---------------------PLLEGIKVVYHPDP-TDALGIAKLVAKHRATILLGTPTFLRLYLRNKkLHPLMFASLRL 902
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  386 CVSAGEPITPDVTEKWRNKTGLDIYEGYGQTET----------VLICGNFKGMKIKPGSMGKPSPAFDVKIVD-VNGNVL 454
Cdd:PRK08633   903 VVAGAEKLKPEVADAFEEKFGIRILEGYGATETspvasvnlpdVLAADFKRQTGSKEGSVGMPLPGVAVRIVDpETFEEL 982
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  455 PPGQEGDI---GIQVLpnrpfglfTHYVDNPSKTASTLR----GNFYITGDRGYMDKDGYFWFVARaddviLSSGYRIG- 526
Cdd:PRK08633   983 PPGEDGLIligGPQVM--------KGYLGDPEKTAEVIKdidgIGWYVTGDKGHLDEDGFLTITDR-----YSRFAKIGg 1049
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  527 ---PF-EVENALNE--HPSVAESAVVSSPDPIRGEVVkafVVLnpdyksHDQEQL-IKEIQEHVKKTTAP--YKyPRKVE 597
Cdd:PRK08633  1050 emvPLgAVEEELAKalGGEEVVFAVTAVPDEKKGEKL---VVL------HTCGAEdVEELKRAIKESGLPnlWK-PSRYF 1119
                          410
                   ....*....|...
gi 1370468924  598 FIQELPKTISGKT 610
Cdd:PRK08633  1120 KVEALPLLGSGKL 1132
PRK07867 PRK07867
acyl-CoA synthetase; Validated
267-622 2.46e-27

acyl-CoA synthetase; Validated


Pssm-ID: 236120 [Multi-domain]  Cd Length: 529  Bit Score: 116.32  E-value: 2.46e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 267 MAIFfTSGTSGYPKMTAHTHSSF-GLGLSVNGRFwlDLTPSDVMWntsdtgwaksawssVFSPWIQGACVFTHHLP---- 341
Cdd:PRK07867  156 MLIF-TSGTSGDPKAVRCTHRKVaSAGVMLAQRF--GLGPDDVCY--------------VSMPLFHSNAVMAGWAValaa 218
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 342 --------RFEPTSILQTLSKYPITVFcsaptvyrmlvqnditSYKFKSLKHCVSA---------------GEPITPDVT 398
Cdd:PRK07867  219 gasialrrKFSASGFLPDVRRYGATYA----------------NYVGKPLSYVLATperpddadnplrivyGNEGAPGDI 282
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 399 EKWRNKTGLDIYEGYGQTETvlicgnfkGMKIK------PGSMGKPSPafDVKIVDVN-GNVLPPGQEGD---------I 462
Cdd:PRK07867  283 ARFARRFGCVVVDGFGSTEG--------GVAITrtpdtpPGALGPLPP--GVAIVDPDtGTECPPAEDADgrllnadeaI 352
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 463 GIQVLPNRPfGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAE 542
Cdd:PRK07867  353 GELVNTAGP-GGFEGYYNDPEADAERMRGGVYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPDATE 431
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 543 SAVVSSPDPIRGEVVKAFVVLNPDyKSHDQEQLIKEIqeHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKEWKT 622
Cdd:PRK07867  432 VAVYAVPDPVVGDQVMAALVLAPG-AKFDPDAFAEFL--AAQPDLGPKQWPSYVRVCAELPRTATFKVLKRQLSAEGVDC 508
PRK13383 PRK13383
acyl-CoA synthetase; Provisional
332-615 1.03e-26

acyl-CoA synthetase; Provisional


Pssm-ID: 139531 [Multi-domain]  Cd Length: 516  Bit Score: 114.32  E-value: 1.03e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 332 GACVFTHHlpRFEPTSILQTLSKYPITVFCSAPTVY-RMLVQNDITSYK--FKSLKHCVSAGEPITPDVTEKWRNKTGLD 408
Cdd:PRK13383  242 GGTVLTHR--HFDAEAALAQASLHRADAFTAVPVVLaRILELPPRVRARnpLPQLRVVMSSGDRLDPTLGQRFMDTYGDI 319
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 409 IYEGYGQTET-VLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVLPNRpfglfTHYVDNPSKtaS 487
Cdd:PRK13383  320 LYNGYGSTEVgIGALATPADLRDAPETVGKPVAGCPVRILDRNNRPVGPRVTGRIFVGGELAG-----TRYTDGGGK--A 392
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 488 TLRGnFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDy 567
Cdd:PRK13383  393 VVDG-MTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHPG- 470
                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*...
gi 1370468924 568 KSHDQEQlikeIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:PRK13383  471 SGVDAAQ----LRDYLKDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
271-566 1.24e-26

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 116.11  E-value: 1.24e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  271 FTSGTSGYPKMTAHTHSSFG-LGLSVNGRFwlDLTPSDVM-WNTS---DTgwakSAWSsVFSPWIQGACVfthHLP---- 341
Cdd:COG1020    624 YTSGSTGRPKGVMVEHRALVnLLAWMQRRY--GLGPGDRVlQFASlsfDA----SVWE-IFGALLSGATL---VLAppea 693
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  342 RFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSykFKSLKHCVSAGEPITPDVTEKWRNKT-GLDIYEGYGQTETVL 420
Cdd:COG1020    694 RRDPAALAELLARHRVTVLNLTPSLLRALLDAAPEA--LPSLRLVLVGGEALPPELVRRWRARLpGARLVNLYGPTETTV 771
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  421 --ICGNFKGMKIKPGSM--GKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQV----LpNRPfGL----FthyVDNPSKT 485
Cdd:COG1020    772 dsTYYEVTPPDADGGSVpiGRPIANTRVYVLDAHLQPVPVGVPGELyigGAGLargyL-NRP-ELtaerF---VADPFGF 846
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  486 ASTlRgnFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNP 565
Cdd:COG1020    847 PGA-R--LYRTGDLARWLPDGNLEFLGRADDQVKIRGFRIELGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEA 923

                   .
gi 1370468924  566 D 566
Cdd:COG1020    924 G 924
PRK12316 PRK12316
peptide synthase; Provisional
72-617 1.28e-26

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 116.21  E-value: 1.28e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924   72 EKAGKKPSNPAFWWinrngEEMRWSFEELGSLSRKFANILseacsLQRG----DRVILILPRVPEWWLANVACLRTGTVL 147
Cdd:PRK12316  4559 ERARMTPDAVAVVF-----DEEKLTYAELNRRANRLAHAL-----IARGvgpeVLVGIAMERSAEMMVGLLAVLKAGGAY 4628
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  148 IPGTTQLTQKDILYRLQSSKANCIITNDVLA---PAVDAVASkcenlhsklivsensregwgnlkelmkvflchpgwstv 224
Cdd:PRK12316  4629 VPLDPEYPRERLAYMMEDSGAALLLTQSHLLqrlPIPDGLAS-------------------------------------- 4670
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  225 arswltatstslvqaiLSLLSSWDYRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWlDLT 304
Cdd:PRK12316  4671 ----------------LALDRDEDWEGFPAHDPAVRLHPDNLAYVIYTSGSTGRPKGVAVSHGSLVNHLHATGERY-ELT 4733
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  305 PSDVMWNTSDTGWAKSAWSsVFSPWIQGACVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFKSLK 384
Cdd:PRK12316  4734 PDDRVLQFMSFSFDGSHEG-LYHPLINGASVVIRDDSLWDPERLYAEIHEHRVTVLVFPPVYLQQLAEHAERDGEPPSLR 4812
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  385 HCVSAGEPITPD-VTEKWRNKTGLDIYEGYGQTETVLICGNFKGMK-IKPGS----MGKPSPAFDVKIVDVNGNVLPPGQ 458
Cdd:PRK12316  4813 VYCFGGEAVAQAsYDLAWRALKPVYLFNGYGPTETTVTVLLWKARDgDACGAaympIGTPLGNRSGYVLDGQLNPLPVGV 4892
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  459 EGD--IGIQvlpnrpfGLFTHYVDNPSKTASTL--------RGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPF 528
Cdd:PRK12316  4893 AGElyLGGE-------GVARGYLERPALTAERFvpdpfgapGGRLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELG 4965
                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  529 EVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQEQ--LIKEIQEHVKKTTAPYKYPRKVEFIQELPKTI 606
Cdd:PRK12316  4966 EIEARLREHPAVREAVVIAQEGAVGKQLVGYVVPQDPALADADEAQaeLRDELKAALRERLPEYMVPAHLVFLARMPLTP 5045
                          570
                   ....*....|.
gi 1370468924  607 SGKTKRNELRK 617
Cdd:PRK12316  5046 NGKLDRKALPQ 5056
PRK05852 PRK05852
fatty acid--CoA ligase family protein;
96-612 1.67e-26

fatty acid--CoA ligase family protein;


Pssm-ID: 235625 [Multi-domain]  Cd Length: 534  Bit Score: 113.83  E-value: 1.67e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITnD 175
Cdd:PRK05852   45 SYRDLARLVDDLAGQLTRS-GLLPGDRVALRMGSNAEFVVALLAASRADLVVVPLDPALPIAEQRVRSQAAGARVVLI-D 122
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 176 VLAPAVDAVASkcenlhsklivsensregwgnlkelmkvflcHPGWSTVARswlTATSTSLVQAILSLlsswdYRHASDS 255
Cdd:PRK05852  123 ADGPHDRAEPT-------------------------------TRWWPLTVN---VGGDSGPSGGTLSV-----HLDAATE 163
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 256 HTCVKT-----KHNEIMaIFFTSGTSGYPKMTAHTHSSfgLGLSVNGRFW-LDLTPSD----VMWNTSDTGWAKSAWSSV 325
Cdd:PRK05852  164 PTPATStpeglRPDDAM-IMFTGGTTGLPKMVPWTHAN--IASSVRAIITgYRLSPRDatvaVMPLYHGHGLIAALLATL 240
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 326 FSpwiqGACVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSY---KFKSLKHCVSAGEPITPDVTEKWR 402
Cdd:PRK05852  241 AS----GGAVLLPARGRFSAHTFWDDIKAVGATWYTAVPTIHQILLERAATEPsgrKPAALRFIRSCSAPLTAETAQALQ 316
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 403 NKTGLDIYEGYGQTET--------VLICGNFKGMKIKPGSMGKpSPAFDVKIVDVNGNVLPPGQEGDIGIqvlpnRPFGL 474
Cdd:PRK05852  317 TEFAAPVVCAFGMTEAthqvtttqIEGIGQTENPVVSTGLVGR-STGAQIRIVGSDGLPLPAGAVGEVWL-----RGTTV 390
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 475 FTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRG 554
Cdd:PRK05852  391 VRGYLGDPTITAANFTDGWLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYG 470
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1370468924 555 EVVKAFVVlnPDYKSHDQEQlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKR 612
Cdd:PRK05852  471 EAVAAVIV--PRESAPPTAE---ELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDR 523
A_NRPS_CmdD_like cd17652
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ...
257-615 2.49e-26

similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).


Pssm-ID: 341307 [Multi-domain]  Cd Length: 436  Bit Score: 111.96  E-value: 2.49e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 257 TCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGlGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSsVFSPWIQGACVf 336
Cdd:cd17652    86 ALLLTTPDNLAYVIYTSGSTGRPKGVVVTHRGLA-NLAAAQIAAFDVGPGSRVLQFASPSFDASVWE-LLMALLAGATL- 162
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 337 thHLPRFEPTS----ILQTLSKYPITVFCSAPTVYRMLVQNDITSykfksLKHCVSAGEPITPDVTEKWrnKTGLDIYEG 412
Cdd:cd17652   163 --VLAPAEELLpgepLADLLREHRITHVTLPPAALAALPPDDLPD-----LRTLVVAGEACPAELVDRW--APGRRMINA 233
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 413 YGQTETVL---ICGNFKGMKIKPgsMGKPSPAFDVKIVDVNGNVLPPGQEGDI---GIqvlpnrpfGLFTHYVDNPSKTA 486
Cdd:cd17652   234 YGPTETTVcatMAGPLPGGGVPP--IGRPVPGTRVYVLDARLRPVPPGVPGELyiaGA--------GLARGYLNRPGLTA 303
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 487 --------STLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVK 558
Cdd:cd17652   304 erfvadpfGAPGSRMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDDRPGDKRLV 383
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1370468924 559 AFVVLNPDyKSHDQEQLikeiQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd17652   384 AYVVPAPG-AAPTAAEL----RAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
FACL_like_5 cd05924
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
269-609 3.82e-26

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341248 [Multi-domain]  Cd Length: 364  Bit Score: 110.16  E-value: 3.82e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPKMTAHTHSSFGLGLS-----VNGRFWLDLTPSDVMWNTSDTGW--------AKSAWSSVFSPWIQGACV 335
Cdd:cd05924     8 ILYTGGTTGMPKGVMWRQEDIFRMLMggadfGTGEFTPSEDAHKAAAAAAGTVMfpapplmhGTGSWTAFGGLLGGQTVV 87
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 336 FthHLPRFEPTSILQTLSKYPITVFCSAPTVY-RMLVQ--NDITSYKFKSLKHCVSAGEPITPDVTEKW-RNKTGLDIYE 411
Cdd:cd05924    88 L--PDDRFDPEEVWRTIEKHKVTSMTIVGDAMaRPLIDalRDAGPYDLSSLFAISSGGALLSPEVKQGLlELVPNITLVD 165
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 412 GYGQTET-VLICGNFKGMKIKPGSMGKPSPafDVKIVDVNGNVLPPGQEGDIGIQVLPNRPFGlfthYVDNPSKTASTLR 490
Cdd:cd05924   166 AFGSSETgFTGSGHSAGSGPETGPFTRANP--DTVVLDDDGRVVPPGSGGVGWIARRGHIPLG----YYGDEAKTAETFP 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 491 ---GNFY-ITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPD 566
Cdd:cd05924   240 evdGVRYaVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDERWGQEVVAVVQLREG 319
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|...
gi 1370468924 567 YKSHDQeqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGK 609
Cdd:cd05924   320 AGVDLE-----ELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
A_NRPS_SidN3_like cd05918
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ...
262-617 1.74e-25

The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341242 [Multi-domain]  Cd Length: 481  Bit Score: 110.32  E-value: 1.74e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 262 KHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFwLDLTPS-----------DVMWNtsdtgwaksawsSVFSPWI 330
Cdd:cd05918   104 SPSDAAYVIFTSGSTGKPKGVVIEHRALSTSALAHGRA-LGLTSEsrvlqfasytfDVSIL------------EIFTTLA 170
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 331 QGACVFT-------HHLPRFeptsilqtLSKYPITVFCSAPTVYRMLVQNDITSykfksLKHCVSAGEPITPDVTEKWRN 403
Cdd:cd05918   171 AGGCLCIpseedrlNDLAGF--------INRLRVTWAFLTPSVARLLDPEDVPS-----LRTLVLGGEALTQSDVDTWAD 237
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 404 KTGLdiYEGYGQTE-TVLICGNFKGMKIKPGSMGKPSPAFdVKIVDVNGN--VLPPGQEGDI---GIQVLPnrpfGlfth 477
Cdd:cd05918   238 RVRL--INAYGPAEcTIAATVSPVVPSTDPRNIGRPLGAT-CWVVDPDNHdrLVPIGAVGELlieGPILAR----G---- 306
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 478 YVDNPSKTA--------------STLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAES 543
Cdd:cd05918   307 YLNDPEKTAaafiedpawlkqegSGRGRRLYRTGDLVRYNPDGSLEYVGRKDTQVKIRGQRVELGEIEHHLRQSLPGAKE 386
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 544 AVVSSPDPIRGEVVK---AFVVLNPDYKSHDQEQ------------LIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISG 608
Cdd:cd05918   387 VVVEVVKPKDGSSSPqlvAFVVLDGSSSGSGDGDslflepsdefraLVAELRSKLRQRLPSYMVPSVFLPLSHLPLTASG 466

                  ....*....
gi 1370468924 609 KTKRNELRK 617
Cdd:cd05918   467 KIDRRALRE 475
A_NRPS_ApnA-like cd17644
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ...
261-615 1.01e-24

similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341299 [Multi-domain]  Cd Length: 465  Bit Score: 107.52  E-value: 1.01e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 261 TKHNEIMAIFFTSGTSGYPKMTAHTHSS---FGLGLSVNgrfwLDLTPSDVMWNTSDTGWAKSAwSSVFSPWIQGAC-VF 336
Cdd:cd17644   103 TQPENLAYVIYTSGSTGKPKGVMIEHQSlvnLSHGLIKE----YGITSSDRVLQFASIAFDVAA-EEIYVTLLSGATlVL 177
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 337 THHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLV----QNDITSykFKSLKHCVSAGEPITPDVTEKWRNKTGLDI--Y 410
Cdd:cd17644   178 RPEEMRSSLEDFVQYIQQWQLTVLSLPPAYWHLLVlellLSTIDL--PSSLRLVIVGGEAVQPELVRQWQKNVGNFIqlI 255
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 411 EGYGQTETVL--ICGNFK---GMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVLpnrpfGLFTHYVDNPSKT 485
Cdd:cd17644   256 NVYGPTEATIaaTVCRLTqltERNITSVPIGRPIANTQVYILDENLQPVPVGVPGELHIGGV-----GLARGYLNRPELT 330
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 486 ASTLRGN---------FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEV 556
Cdd:cd17644   331 AEKFISHpfnsseserLYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQPGNKR 410
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1370468924 557 VKAFVVlnPDYkshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd17644   411 LVAYIV--PHY---EESPSTVELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
A_NRPS_Sfm_like cd12115
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ...
269-615 2.86e-24

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.


Pssm-ID: 341280 [Multi-domain]  Cd Length: 447  Bit Score: 106.25  E-value: 2.86e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPKMTAHTHSSfglglSVNGRFWLDLT-PSD----VMWNTSdTGWAKSAWSsVFSPWIQGACVFT------ 337
Cdd:cd12115   110 VIYTSGSTGRPKGVAIEHRN-----AAAFLQWAAAAfSAEelagVLASTS-ICFDLSVFE-LFGPLATGGKVVLadnvla 182
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 338 -HHLPRFEPTSILQTLskypitvfcsaPTVYRMLVQNDITSykfKSLKHCVSAGEPITPD-VTEKWRNKTGLDIYEGYGQ 415
Cdd:cd12115   183 lPDLPAAAEVTLINTV-----------PSAAAELLRHDALP---ASVRVVNLAGEPLPRDlVQRLYARLQVERVVNLYGP 248
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 416 TE-----TVLICGnfKGMKIKPgSMGKPSPAFDVKIVDVNGNVLPPGQEGDI---GIqvlpnrpfGLFTHYVDNPSKTAS 487
Cdd:cd12115   249 SEdttysTVAPVP--PGASGEV-SIGRPLANTQAYVLDRALQPVPLGVPGELyigGA--------GVARGYLGRPGLTAE 317
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 488 TLRGN-------FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAF 560
Cdd:cd12115   318 RFLPDpfgpgarLYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALRSIPGVREAVVVAIGDAAGERRLVAY 397
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1370468924 561 VVLNPDYKShdqeqLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd12115   398 IVAEPGAAG-----LVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
PRK09274 PRK09274
peptide synthase; Provisional
96-582 5.96e-24

peptide synthase; Provisional


Pssm-ID: 236443 [Multi-domain]  Cd Length: 552  Bit Score: 106.14  E-value: 5.96e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTG--TVLI-PGttqLTQKDILYRLQSSKANCII 172
Cdd:PRK09274   43 SFAELDARSDAIAHGLNAA-GIGRGMRAVLMVTPSLEFFALTFALFKAGavPVLVdPG---MGIKNLKQCLAEAQPDAFI 118
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 173 TNDvLAPAVdavaskcenlhSKLIvsensreGWGnlKELMKVFLchpgwsTVARSWLTAtSTSLVQAILsllsswdyRHA 252
Cdd:PRK09274  119 GIP-KAHLA-----------RRLF-------GWG--KPSVRRLV------TVGGRLLWG-GTTLATLLR--------DGA 162
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 253 SDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSF---------GLGLSVNGRfwlDLTPSDVMwntsdtgwaksaws 323
Cdd:PRK09274  163 AAPFPMADLAPDDMAAILFTSGSTGTPKGVVYTHGMFeaqiealreDYGIEPGEI---DLPTFPLF-------------- 225
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 324 SVFSPWIQGACVfthhLPRFEPT--------SILQTLSKYPITVFCSAPTVYRML----VQNDItsyKFKSLKHCVSAGE 391
Cdd:PRK09274  226 ALFGPALGMTSV----IPDMDPTrpatvdpaKLFAAIERYGVTNLFGSPALLERLgrygEANGI---KLPSLRRVISAGA 298
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 392 PITPDVTEKWRN--KTGLDIYEGYGQTETVLICgnfkgmKIkpGS------------------MGKPSPAFDVKIVDVNG 451
Cdd:PRK09274  299 PVPIAVIERFRAmlPPDAEILTPYGATEALPIS------SI--ESreilfatraatdngagicVGRPVDGVEVRIIAISD 370
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 452 N---------VLPPGQEGDI---GIQVLPNrpfglfthYVDNPSKTA-----STLRGNFYITGDRGYMDKDGYFWFVAR- 513
Cdd:PRK09274  371 ApipewddalRLATGEIGEIvvaGPMVTRS--------YYNRPEATRlakipDGQGDVWHRMGDLGYLDAQGRLWFCGRk 442
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 514 ADDVILSSGyRIGPFEVENALNEHPSVAESAVVSSPDPirGEVVKAFVV-LNPDyKSHDQEQLIKEIQEH 582
Cdd:PRK09274  443 AHRVETAGG-TLYTIPCERIFNTHPGVKRSALVGVGVP--GAQRPVLCVeLEPG-VACSKSALYQELRAL 508
PRK12467 PRK12467
peptide synthase; Provisional
269-619 7.76e-24

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 107.55  E-value: 7.76e-24
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  269 IFFTSGTSGYPKMTAHTHSSFGLGLSVNGRfWLDLTPSDVMWNTSDTGWAKSAWSsVFSPWIQGACVftHHLPR---FEP 345
Cdd:PRK12467   661 VIYTSGSTGQPKGVAISHGALANYVCVIAE-RLQLAADDSMLMVSTFAFDLGVTE-LFGALASGATL--HLLPPdcaRDA 736
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  346 TSILQTLSKYPITVFCSAPTVYRMLVQnDITSYKFKSLKHCVSAGEPITPDVTEKWRNKT-GLDIYEGYGQTETVLICGN 424
Cdd:PRK12467   737 EAFAALMADQGVTVLKIVPSHLQALLQ-ASRVALPRPQRALVCGGEALQVDLLARVRALGpGARLINHYGPTETTVGVST 815
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  425 FK----GMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGD--IGIQVLPN----RPFGLFTHYVDNPSKTAStlrGNFY 494
Cdd:PRK12467   816 YElsdeERDFGNVPIGQPLANLGLYILDHYLNPVPVGVVGElyIGGAGLARgyhrRPALTAERFVPDPFGADG---GRLY 892
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  495 ITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVkAFVVlnPDYKSHDQEQ 574
Cdd:PRK12467   893 RTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLAQPGDAGLQLV-AYLV--PAAVADGAEH 969
                          330       340       350       360
                   ....*....|....*....|....*....|....*....|....*..
gi 1370468924  575 LIK--EIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKE 619
Cdd:PRK12467   970 QATrdELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPKPD 1016
PRK05857 PRK05857
fatty acid--CoA ligase;
72-615 2.48e-23

fatty acid--CoA ligase;


Pssm-ID: 180293 [Multi-domain]  Cd Length: 540  Bit Score: 104.32  E-value: 2.48e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  72 EKAGKKPSNPAfwwINRNGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVlipgt 151
Cdd:PRK05857   22 EQARQQPEAIA---LRRCDGTSALRYRELVAEVGGLAADL-RAQSVSRGSRVLVISDNGPETYLSVLACAKLGAI----- 92
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 152 tqltqkdilyrlqsskanCIITNDVLAPAvdAVASKCENLH-SKLIVSENSREGWGNLKELMKVFlchPGWSTVARSWLT 230
Cdd:PRK05857   93 ------------------AVMADGNLPIA--AIERFCQITDpAAALVAPGSKMASSAVPEALHSI---PVIAVDIAAVTR 149
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 231 ATSTSLVQAILSllsswdyrhasdshTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLG---LSVNGRFWLDltpsd 307
Cdd:PRK05857  150 ESEHSLDAASLA--------------GNADQGSEDPLAMIFTSGTTGEPKAVLLANRTFFAVpdiLQKEGLNWVT----- 210
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 308 vmWNTSDTGWAKSAWSSVFSPW------IQGACVFTHHlprfEPT-SILQTLSKYPITVFCSAPTVYRMLVQN-DITSYK 379
Cdd:PRK05857  211 --WVVGETTYSPLPATHIGGLWwiltclMHGGLCVTGG----ENTtSLLEILTTNAVATTCLVPTLLSKLVSElKSANAT 284
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 380 FKSLKHCVSAG-EPITPDVteKWRNKTGLDIYEGYGQTET-----VLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNG-- 451
Cdd:PRK05857  285 VPSLRLVGYGGsRAIAADV--RFIEATGVRTAQVYGLSETgctalCLPTDDGSIVKIEAGAVGRPYPGVDVYLAATDGig 362
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 452 -NVLPPGQEGDIGIQVL--PNRPFGlfthYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPF 528
Cdd:PRK05857  363 pTAPGAGPSASFGTLWIksPANMLG----YWNNPERTAEVLIDGWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPD 438
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 529 EVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISG 608
Cdd:PRK05857  439 EVDRIAEGVSGVREAACYEIPDEEFGALVGLAVVASAELDESAARALKHTIAARFRRESEPMARPSTIVIVTDIPRTQSG 518

                  ....*..
gi 1370468924 609 KTKRNEL 615
Cdd:PRK05857  519 KVMRASL 525
PRK12467 PRK12467
peptide synthase; Provisional
269-621 6.17e-23

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 104.47  E-value: 6.17e-23
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  269 IFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWlDLTPSDVMWNTSDTGWAKSAWSsVFSPWIQGACVFTHHLPRFEPTSI 348
Cdd:PRK12467  3242 VIYTSGSTGKPKGVGVRHGALANHLCWIAEAY-ELDANDRVLLFMSFSFDGAQER-FLWTLICGGCLVVRDNDLWDPEEL 3319
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  349 LQTLSKYPITVFCSAPTVYRMLVQN-DITSYKfkSLKHCVSAGEPITPDVTEKWRNKTG-LDIYEGYGQTETVLI----- 421
Cdd:PRK12467  3320 WQAIHAHRISIACFPPAYLQQFAEDaGGADCA--SLDIYVFGGEAVPPAAFEQVKRKLKpRGLTNGYGPTEAVVTvtlwk 3397
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  422 CGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVLpnrpfGLFTHYVDNPSKTA--------STLRGNF 493
Cdd:PRK12467  3398 CGGDAVCEAPYAPIGRPVAGRSIYVLDGQLNPVPVGVAGELYIGGV-----GLARGYHQRPSLTAerfvadpfSGSGGRL 3472
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  494 YITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSpDPIRGEVVKAFVVLNPdykshDQE 573
Cdd:PRK12467  3473 YRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVREAVVLAR-DGAGGKQLVAYVVPAD-----PQG 3546
                          330       340       350       360
                   ....*....|....*....|....*....|....*....|....*...
gi 1370468924  574 QLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKEWK 621
Cdd:PRK12467  3547 DWRETLRDHLAASLPDYMVPAQLLVLAAMPLGPNGKVDRKALPDPDAK 3594
A_NRPS_TubE_like cd05906
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ...
86-617 7.15e-23

The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341232 [Multi-domain]  Cd Length: 540  Bit Score: 102.75  E-value: 7.15e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  86 INRNGEEMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPR----VPEWWlanvACLRTGTVLIPGTTQLTqkdilY 161
Cdd:cd05906    31 IDADGSEEFQSYQDLLEDARRLAAGLRQL-GLRPGDSVILQFDDnedfIPAFW----ACVLAGFVPAPLTVPPT-----Y 100
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 162 RLQSSKAN------------CIITNDVLAPAVDAVASKCENLHSKLIVSENSREGWGNlkelmkvflcHPgwstvarsWL 229
Cdd:cd05906   101 DEPNARLRklrhiwqllgspVVLTDAELVAEFAGLETLSGLPGIRVLSIEELLDTAAD----------HD--------LP 162
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 230 TATSTSLVqailsllsswdyrhasdshtcvktkhneimAIFFTSGTSGYPKMTAHTHSSF---GLGLSVNGRFwldlTPS 306
Cdd:cd05906   163 QSRPDDLA------------------------------LLMLTSGSTGFPKAVPLTHRNIlarSAGKIQHNGL----TPQ 208
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 307 DVMWNtsdtgwaksawssvfspWIQ----GACVFTHHLPRF---------------EPTSILQTLSKYPITV-FcsAPT- 365
Cdd:cd05906   209 DVFLN-----------------WVPldhvGGLVELHLRAVYlgcqqvhvpteeilaDPLRWLDLIDRYRVTItW--APNf 269
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 366 VYRMLVQ----NDITSYKFKSLKHCVSAGEPITPDVTEKWRN---KTGLD---IYEGYGQTET---VLICGNFKGMKIKP 432
Cdd:cd05906   270 AFALLNDlleeIEDGTWDLSSLRYLVNAGEAVVAKTIRRLLRllePYGLPpdaIRPAFGMTETcsgVIYSRSFPTYDHSQ 349
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 433 G----SMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVLPnrpfgLFTHYVDNPSKTASTLR-GNFYITGDRGYMDkDGY 507
Cdd:cd05906   350 AlefvSLGRPIPGVSMRIVDDEGQLLPEGEVGRLQVRGPV-----VTKGYYNNPEANAEAFTeDGWFRTGDLGFLD-NGN 423
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 508 FWFVARADDVILSSGYRIGPFEVENALNEHPSVAES--AVVSSPDPIRGEVVKAfVVLNPDYKSHDQ-EQLIKEIQEHV- 583
Cdd:cd05906   424 LTITGRTKDTIIVNGVNYYSHEIEAAVEEVPGVEPSftAAFAVRDPGAETEELA-IFFVPEYDLQDAlSETLRAIRSVVs 502
                         570       580       590
                  ....*....|....*....|....*....|....*...
gi 1370468924 584 KKTTAPYKY----PRkvefiQELPKTISGKTKRNELRK 617
Cdd:cd05906   503 REVGVSPAYliplPK-----EEIPKTSLGKIQRSKLKA 535
PRK13388 PRK13388
acyl-CoA synthetase; Provisional
243-622 7.75e-23

acyl-CoA synthetase; Provisional


Pssm-ID: 237374 [Multi-domain]  Cd Length: 540  Bit Score: 102.41  E-value: 7.75e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 243 LLSSWDYRHASDSHTCVkTKHNEIMA-----IFFTSGTSGYPKMTAHTHSSFG-LGLSVNGRFwlDLTPSDVMW------ 310
Cdd:PRK13388  125 DVDTPAYAELVAAAGAL-TPHREVDAmdpfmLIFTSGTTGAPKAVRCSHGRLAfAGRALTERF--GLTRDDVCYvsmplf 201
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 311 --NTSDTGWAKSAwssvfspwIQGACVFTHhlPRFEPTSILQTLSKYPITVF--CSAPTVYRMLV-------QNDITsyk 379
Cdd:PRK13388  202 hsNAVMAGWAPAV--------ASGAAVALP--AKFSASGFLDDVRRYGATYFnyVGKPLAYILATperpddaDNPLR--- 268
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 380 fkslkhcVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETVLICGNFKGMKikPGSMGKPSPafDVKIVDV---------- 449
Cdd:PRK13388  269 -------VAFGNEASPRDIAEFSRRFGCQVEDGYGSSEGAVIVVREPGTP--PGSIGRGAP--GVAIYNPetltecavar 337
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 450 ---NGNVLPPgqegDIGIQVLPNRP-FGLFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRI 525
Cdd:PRK13388  338 fdaHGALLNA----DEAIGELVNTAgAGFFEGYYNNPEATAERMRHGMYWSGDLAYRDADGWIYFAGRTADWMRVDGENL 413
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 526 GPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKS---------HDQEQLikeiqehvkkttAPYKYPRKV 596
Cdd:PRK13388  414 SAAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGATFdpdafaaflAAQPDL------------GTKAWPRYV 481
                         410       420
                  ....*....|....*....|....*.
gi 1370468924 597 EFIQELPKTISGKTKRNELRKKEWKT 622
Cdd:PRK13388  482 RIAADLPSTATNKVLKRELIAQGWAT 507
PRK05620 PRK05620
long-chain fatty-acid--CoA ligase;
96-617 8.27e-23

long-chain fatty-acid--CoA ligase;


Pssm-ID: 180167 [Multi-domain]  Cd Length: 576  Bit Score: 102.55  E-value: 8.27e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  96 SFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEWW--LANVACLrtGTVLIPGTTQLTQKDILYRLQSSKANCIIT 173
Cdd:PRK05620   40 TFAAIGARAAALAHALHDELGITGDQRVGSMMYNCAEHLevLFAVACM--GAVFNPLNKQLMNDQIVHIINHAEDEVIVA 117
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 174 NDVLAPAVDAVASKCENLHSKLIVSENSregwgnlkelmkvflchpgwSTVARSwltatstslvqAILSLLSSWDYRHAS 253
Cdd:PRK05620  118 DPRLAEQLGEILKECPCVRAVVFIGPSD--------------------ADSAAA-----------HMPEGIKVYSYEALL 166
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 254 DSHTCV----KTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLsvngrfwLDLTPSDVMWNTSDTG-------WAKSAW 322
Cdd:PRK05620  167 DGRSTVydwpELDETTAAAICYSTGTTGAPKGVVYSHRSLYLQS-------LSLRTTDSLAVTHGESflccvpiYHVLSW 239
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 323 SSVFSPWIQGA-CVFTHHlpRFEPTSILQTLSKYPITVFCSAPTVY-RMLVQNDITSYKFKSLKHCVSAGEPITPDVTEK 400
Cdd:PRK05620  240 GVPLAAFMSGTpLVFPGP--DLSAPTLAKIIATAMPRVAHGVPTLWiQLMVHYLKNPPERMSLQEIYVGGSAVPPILIKA 317
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 401 WRNKTGLDIYEGYGQTETVLIcgnfkGMKIKPG-------------SMGKPSPAFDVKIVDvNGNVLPPGQEGDIGIQVL 467
Cdd:PRK05620  318 WEERYGVDVVHVWGMTETSPV-----GTVARPPsgvsgearwayrvSQGRFPASLEYRIVN-DGQVMESTDRNEGEIQVR 391
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 468 PNRpfgLFTHYVDNPSKT----ASTLRGN-------------FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEV 530
Cdd:PRK05620  392 GNW---VTASYYHSPTEEgggaASTFRGEdvedandrftadgWLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQL 468
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 531 ENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQ--EQLIKEIQEHVKKTTAPYKYprkvEFIQELPKTISG 608
Cdd:PRK05620  469 ENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTREtaERLRDQLRDRLPNWMLPEYW----TFVDEIDKTSVG 544

                  ....*....
gi 1370468924 609 KTKRNELRK 617
Cdd:PRK05620  545 KFDKKDLRQ 553
LC_FACS_like cd17640
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ...
249-546 2.34e-22

Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341295 [Multi-domain]  Cd Length: 468  Bit Score: 100.51  E-value: 2.34e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 249 YRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSF---------GLGLSVNGRFwLDLTPSdvmWNTSDtgwaK 319
Cdd:cd17640    73 LNHSESVALVVENDSDDLATIIYTSGTTGNPKGVMLTHANLlhqirslsdIVPPQPGDRF-LSILPI---WHSYE----R 144
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 320 SAWSSVFSpwiQG-ACVFThhlprfEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFKS---------------L 383
Cdd:cd17640   145 SAEYFIFA---CGcSQAYT------SIRTLKDDLKRVKPHYIVSVPRLWESLYSGIQKQVSKSSpikqflflfflsggiF 215
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 384 KHCVSAGEPITPDVtEKWRNKTGLDIYEGYGQTET--VLICGNFKGMKIkpGSMGKPSPAFDVKIVDVNGN-VLPPGQEG 460
Cdd:cd17640   216 KFGISGGGALPPHV-DTFFEAIGIEVLNGYGLTETspVVSARRLKCNVR--GSVGRPLPGTEIKIVDPEGNvVLPPGEKG 292
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 461 DI---GIQVLPNrpfglfthYVDNPSKTASTLRGN-FYITGDRGYMDKDGYFWFVARADDVI-LSSGYRIGPFEVENALN 535
Cdd:cd17640   293 IVwvrGPQVMKG--------YYKNPEATSKVLDSDgWFNTGDLGWLTCGGELVLTGRAKDTIvLSNGENVEPQPIEEALM 364
                         330
                  ....*....|.
gi 1370468924 536 EHPSVAESAVV 546
Cdd:cd17640   365 RSPFIEQIMVV 375
PRK04813 PRK04813
D-alanine--poly(phosphoribitol) ligase subunit DltA;
350-618 2.60e-22

D-alanine--poly(phosphoribitol) ligase subunit DltA;


Pssm-ID: 235313 [Multi-domain]  Cd Length: 503  Bit Score: 100.74  E-value: 2.60e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 350 QTLSKYPITVFCSAPTVYRM-LVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNK-TGLDIYEGYGQTE-TVLIcgnfK 426
Cdd:PRK04813  228 ETLPQLPINVWVSTPSFADMcLLDPSFNEEHLPNLTHFLFCGEELPHKTAKKLLERfPSATIYNTYGPTEaTVAV----T 303
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 427 GMKI--------KPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIqVLPNRPFGlfthYVDNPSKTAS---TLRGN-FY 494
Cdd:PRK04813  304 SIEItdemldqyKRLPIGYAKPDSPLLIIDEEGTKLPDGEQGEIVI-SGPSVSKG----YLNNPEKTAEaffTFDGQpAY 378
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 495 ITGDRGYMDkDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVsspdPI-RGEVVK---AFVVLNPdyksH 570
Cdd:PRK04813  379 HTGDAGYLE-DGLLFYQGRIDFQIKLNGYRIELEEIEQNLRQSSYVESAVVV----PYnKDHKVQyliAYVVPKE----E 449
                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1370468924 571 DQE---QLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKK 618
Cdd:PRK04813  450 DFErefELTKAIKKELKERLMEYMIPRKFIYRDSLPLTPNGKIDRKALIEE 500
entE PRK10946
(2,3-dihydroxybenzoyl)adenylate synthase;
436-617 2.93e-22

(2,3-dihydroxybenzoyl)adenylate synthase;


Pssm-ID: 236803 [Multi-domain]  Cd Length: 536  Bit Score: 100.83  E-value: 2.93e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 436 GKP-SPAFDVKIVDVNGNVLPPGQEGdigiqVLPNR-PFgLFTHYVDNPSKTASTLRGN-FYITGDRGYMDKDGYFWFVA 512
Cdd:PRK10946  356 GRPmSPDDEVWVADADGNPLPQGEVG-----RLMTRgPY-TFRGYYKSPQHNASAFDANgFYCSGDLVSIDPDGYITVVG 429
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 513 RADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKS-----HDQEQLIkeiqehvkktt 587
Cdd:PRK10946  430 REKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVKEPLKAvqlrrFLREQGI----------- 498
                         170       180       190
                  ....*....|....*....|....*....|
gi 1370468924 588 APYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK10946  499 AEFKLPDRVECVDSLPLTAVGKVDKKQLRQ 528
LC_FACS_bac cd05932
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ...
89-618 4.11e-22

Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341255 [Multi-domain]  Cd Length: 508  Bit Score: 100.24  E-value: 4.11e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  89 NGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKA 168
Cdd:cd05932     1 GGQVVEFTWGEVADKARRLAAAL-RALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSES 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 169 NCIIT-----NDVLAPAV-DAVASKCENLHSKLivseNSREGWGNLKElmkvflCHPgwstvarswltatstslvqails 242
Cdd:cd05932    80 KALFVgklddWKAMAPGVpEGLISISLPPPSAA----NCQYQWDDLIA------QHP----------------------- 126
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 243 lLSSWDYRHASDshtcvktkhnEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSvNGRFWLDLTPSDVMWntSDTGWAKSAW 322
Cdd:cd05932   127 -PLEERPTRFPE----------QLATLIYTSGTTGQPKGVMLTFGSFAWAAQ-AGIEHIGTEENDRML--SYLPLAHVTE 192
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 323 SS-VFSPWIQGACV--FTHHLPRFeptsiLQTLSKYPITVFCSAP---TVYRMLVQNDITSYKFKSL----------KHC 386
Cdd:cd05932   193 RVfVEGGSLYGGVLvaFAESLDTF-----VEDVQRARPTLFFSVPrlwTKFQQGVQDKIPQQKLNLLlkipvvnslvKRK 267
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 387 VSAG-------------EPITPDVTEkWRNKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIvdvngnv 453
Cdd:cd05932   268 VLKGlgldqcrlagcgsAPVPPALLE-WYRSLGLNILEAYGMTENFAYSHLNYPGRDKIGTVGNAGPGVEVRI------- 339
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 454 lppGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGN-FYITGDRGYMDKDGYFWFVARADDVILSS-GYRIGPFEVE 531
Cdd:cd05932   340 ---SEDGEILV-----RSPALMMGYYKDPEATAEAFTADgFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIE 411
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 532 NALNEHPSVAESAVVSS--PDPIRGEVVKAFVVLNPDYKSHDQ-EQLIKEIQEHVKKTTAPYKYPRKVEFIQElPKTISG 608
Cdd:cd05932   412 NKLAEHDRVEMVCVIGSglPAPLALVVLSEEARLRADAFARAElEASLRAHLARVNSTLDSHEQLAGIVVVKD-PWSIDN 490
                         570
                  ....*....|....*..
gi 1370468924 609 -------KTKRNELRKK 618
Cdd:cd05932   491 giltptlKIKRNVLEKA 507
PRK12316 PRK12316
peptide synthase; Provisional
233-617 4.13e-22

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 101.96  E-value: 4.13e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  233 STSLVQAILSLLSSWDYRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFwLDLTPSDVMWNT 312
Cdd:PRK12316  3165 RLPLAQGVQVLDLDRGDENYAEANPAIRTMPENLAYVIYTSGSTGKPKGVGIRHSALSNHLCWMQQA-YGLGVGDRVLQF 3243
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  313 SDTGWAKSAWSsVFSPWIQGACVFTHHLPRF-EPTSILQTLSKYPITVFCSAPTVYRMLVQnDITSYKFKSLKHCVSAGE 391
Cdd:PRK12316  3244 TTFSFDVFVEE-LFWPLMSGARVVLAGPEDWrDPALLVELINSEGVDVLHAYPSMLQAFLE-EEDAHRCTSLKRIVCGGE 3321
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  392 PITPDVTEKWrnKTGLDIYEGYGQTETVLICGNFKGMKIKPGS--MGKPSPAFDVKIVDVNGNVLPPGQEGD--IGIQVL 467
Cdd:PRK12316  3322 ALPADLQQQV--FAGLPLYNLYGPTEATITVTHWQCVEEGKDAvpIGRPIANRACYILDGSLEPVPVGALGElyLGGEGL 3399
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  468 P----NRPFGLFTHYVDNPSKTASTLrgnfYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAES 543
Cdd:PRK12316  3400 ArgyhNRPGLTAERFVPDPFVPGERL----YRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREA 3475
                          330       340       350       360       370       380       390
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1370468924  544 AVVSspdpIRGEVVKAFVVLnpdykSHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK12316  3476 VVLA----VDGRQLVAYVVP-----EDEAGDLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPR 3540
ttLC_FACS_like cd05915
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
267-617 2.70e-21

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.


Pssm-ID: 213283 [Multi-domain]  Cd Length: 509  Bit Score: 97.50  E-value: 2.70e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 267 MAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRF-WLDLTPSDVMWNTSDTgWAKSAWSSVFS-PWIQGACVFTHHLPRFE 344
Cdd:cd05915   156 CGMAYTTGTTGLPKGVVYSHRALVLHSLAASLVdGTALSEKDVVLPVVPM-FHVNAWCLPYAaTLVGAKQVLPGPRLDPA 234
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 345 ptSILQTLSKYPITVFCSAPTVYRMLVQ-NDITSYKFKSLKHCVSAGEPiTPDVTEKWRNKTGLDIYEGYGQTETVLI-- 421
Cdd:cd05915   235 --SLVELFDGEGVTFTAGVPTVWLALADyLESTGHRLKTLRRLVVGGSA-APRSLIARFERMGVEVRQGYGLTETSPVvv 311
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 422 ----------CGNFKGMKIK----------------PGSMGKPSPAFDVKIVDVNGNVLPPGqegdigiqvlpnrpfglf 475
Cdd:cd05915   312 qnfvkshlesLSEEEKLTLKaktglpiplvrlrvadEEGRPVPKDGKALGEVQLKGPWITGG------------------ 373
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 476 tHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGE 555
Cdd:cd05915   374 -YYGNEEATRSALTPDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQE 452
                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1370468924 556 VVKAFVVLNpdykshDQEQLIKEIQEHVKKTTAPYKY-PRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05915   453 RPLAVVVPR------GEKPTPEELNEHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALRE 509
LC_FACS_bac1 cd17641
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ...
95-565 1.23e-20

bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341296 [Multi-domain]  Cd Length: 569  Bit Score: 95.95  E-value: 1.23e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  95 WSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITN 174
Cdd:cd17641    12 FTWADYADRVRAFALGLL-ALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIAE 90
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 175 DvlAPAVDavaskcenlhsKLIvsensrEGWGNLKELMKVFLCHP-GWSTVARSWLTAtstslVQAILSLLSSWDYRHAS 253
Cdd:cd17641    91 D--EEQVD-----------KLL------EIADRIPSVRYVIYCDPrGMRKYDDPRLIS-----FEDVVALGRALDRRDPG 146
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 254 DSHTCVKTKHNEIMAIF-FTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQG 332
Cdd:cd17641   147 LYEREVAAGKGEDVAVLcTTSGTTGKPKLAMLSHGNF-LGHCAAYLAADPLGPGDEYVSVLPLPWIGEQMYSVGQALVCG 225
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 333 ACVfthHLPRfEPTSILQTLSKYPITVFCSAPTVYRMLVQN------DITSYK--------------------------- 379
Cdd:cd17641   226 FIV---NFPE-EPETMMEDLREIGPTFVLLPPRVWEGIAADvrarmmDATPFKrfmfelgmklglraldrgkrgrpvslw 301
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 380 ---------------------FKSLKHCVSAGEPITPDVTEKWRnKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKP 438
Cdd:cd17641   302 lrlaswladallfrplrdrlgFSRLRSAATGGAALGPDTFRFFH-AIGVPLKQLYGQTELAGAYTVHRDGDVDPDTVGVP 380
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 439 SPAFDVKIVDVnGNVLPPGQegdigiqvlpnrpfGLFTHYVDNPSKTASTL-RGNFYITGDRGYMDKDGYFWFVARADDV 517
Cdd:cd17641   381 FPGTEVRIDEV-GEILVRSP--------------GVFVGYYKNPEATAEDFdEDGWLHTGDAGYFKENGHLVVIDRAKDV 445
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....*....
gi 1370468924 518 -ILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIrgevVKAFVVLNP 565
Cdd:cd17641   446 gTTSDGTRFSPQFIENKLKFSPYIAEAVVLGAGRPY----LTAFICIDY 490
A_NRPS_TlmIV_like cd12114
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ...
94-615 1.42e-20

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.


Pssm-ID: 341279 [Multi-domain]  Cd Length: 477  Bit Score: 95.03  E-value: 1.42e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  94 RWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIIT 173
Cdd:cd12114    12 TLTYGELAERARRVAGAL-KAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARREAILADAGARLVLT 90
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 174 NDVLAPAVDAVAskcenlhsklivsensregwgnlkelmkvflchpgwstvarswlTATSTSLVQAILSLLSSWDYRHAS 253
Cdd:cd12114    91 DGPDAQLDVAVF--------------------------------------------DVLILDLDALAAPAPPPPVDVAPD 126
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 254 DshtcvktkhneiMA-IFFTSGTSGYPKMTAHTHSS-FGLGLSVNGRFwlDLTPSDVMWNTSDTGWAKSAWSsVFSPWIQ 331
Cdd:cd12114   127 D------------LAyVIFTSGSTGTPKGVMISHRAaLNTILDINRRF--AVGPDDRVLALSSLSFDLSVYD-IFGALSA 191
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 332 GAC-VFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLV----QNDITSykfKSLKHCVSAGEPITPDVTEKWRNKT- 405
Cdd:cd12114   192 GATlVLPDEARRRDPAHWAELIERHGVTLWNSVPALLEMLLdvleAAQALL---PSLRLVLLSGDWIPLDLPARLRALAp 268
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 406 GLDIYEGYGQTETVlICGNFkgMKIKPGSM-------GKPSPAFDVKIVDVNGNVLPPGQEGDI---GIqvlpnrpfGLF 475
Cdd:cd12114   269 DARLISLGGATEAS-IWSIY--HPIDEVPPdwrsipyGRPLANQRYRVLDPRGRDCPDWVPGELwigGR--------GVA 337
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 476 THYVDNPSKTAS-----TLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPD 550
Cdd:cd12114   338 LGYLGDPELTAArfvthPDGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVARAVVVVLGD 417
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1370468924 551 PiRGEVVKAFVVLNPDYKSHDQEqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd12114   418 P-GGKRLAAFVVPDNDGTPIAPD----ALRAFLAQTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
A_NRPS_ACVS-like cd17648
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ...
269-609 1.71e-20

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.


Pssm-ID: 341303 [Multi-domain]  Cd Length: 453  Bit Score: 94.77  E-value: 1.71e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPK--MTAHtHSSFGLGLSVNGRFWLDLTPSDVMWNTSdtgwaksawSSVFSPWIQGAC--VFTHH----L 340
Cdd:cd17648    99 AIYTSGTTGKPKgvLVEH-GSVVNLRTSLSERYFGRDNGDEAVLFFS---------NYVFDFFVEQMTlaLLNGQklvvP 168
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 341 P---RFEPTSILQTLSKYPITVFCSAPTVyrmLVQNDITSykFKSLKHCVSAGEPITPDVTEKWRNKTGLDIYEGYGQTE 417
Cdd:cd17648   169 PdemRFDPDRFYAYINREKVTYLSGTPSV---LQQYDLAR--LPHLKRVDAAGEEFTAPVFEKLRSRFAGLIINAYGPTE 243
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 418 TVL--ICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDI---GIQVLP---NRPFGLFTHYVDNPSKTAS-T 488
Cdd:cd17648   244 TTVtnHKRFFPGDQRFDKSLGRPVRNTKCYVLNDAMKRVPVGAVGELylgGDGVARgylNRPELTAERFLPNPFQTEQeR 323
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 489 LRGNF---YITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDP-IRGEVVKAFVVln 564
Cdd:cd17648   324 ARGRNarlYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVVAKEDAsQAQSRIQKYLV-- 401
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*.
gi 1370468924 565 pDYKSHDQEQLIK-EIQEHVKKTTAPYKYPRKVEFIQELPKTISGK 609
Cdd:cd17648   402 -GYYLPEPGHVPEsDLLSFLRAKLPRYMVPARLVRLEGIPVTINGK 446
A_NRPS_LgrA-like cd17645
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ...
252-615 2.60e-20

adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.


Pssm-ID: 341300 [Multi-domain]  Cd Length: 440  Bit Score: 94.16  E-value: 2.60e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 252 ASDSHTCVKTKHNEIMA-IFFTSGTSGYPKMTAHTHSSFglglsVNGRFW----LDLTPSDVMWNTSDTGWAKSAWSsVF 326
Cdd:cd17645    91 LADSSAKILLTNPDDLAyVIYTSGSTGLPKGVMIEHHNL-----VNLCEWhrpyFGVTPADKSLVYASFSFDASAWE-IF 164
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 327 SPWIQGACVftHHLP---RFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSykfksLKHCVSAGepitpDVTEKWRN 403
Cdd:cd17645   165 PHLTAGAAL--HVVPserRLDLDALNDYFNQEGITISFLPTGAAEQFMQLDNQS-----LRVLLTGG-----DKLKKIER 232
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 404 KtGLDIYEGYGQTETVLICGNFKGMKIKPG-SMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvlpnrPFGLFTHYVDNP 482
Cdd:cd17645   233 K-GYKLVNNYGPTENTVVATSFEIDKPYANiPIGKPIDNTRVYILDEALQLQPIGVAGELCIA-----GEGLARGYLNRP 306
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 483 SKTASTLRGN-------FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGE 555
Cdd:cd17645   307 ELTAEKFIVHpfvpgerMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGRK 386
                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 556 VVKAFVVLnpdykshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd17645   387 YLVAYVTA-------PEEIPHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
PRK07445 PRK07445
O-succinylbenzoic acid--CoA ligase; Reviewed
413-617 2.66e-20

O-succinylbenzoic acid--CoA ligase; Reviewed


Pssm-ID: 236019 [Multi-domain]  Cd Length: 452  Bit Score: 93.91  E-value: 2.66e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 413 YGQTETV-LICgnfkgmKIKPG-------SMGKPSPAFDVKIVdvngnvlpPGQEGDIGIQVlPNRPFGLFTHYVDNPsk 484
Cdd:PRK07445  261 YGMTETAsQIA------TLKPDdflagnnSSGQVLPHAQITIP--------ANQTGNITIQA-QSLALGYYPQILDSQ-- 323
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 485 tastlrgNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVln 564
Cdd:PRK07445  324 -------GIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYV-- 394
                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1370468924 565 PDYKSHDQEqlikEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK07445  395 PKDPSISLE----ELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQ 443
PRK06018 PRK06018
putative acyl-CoA synthetase; Provisional
268-618 5.03e-20

putative acyl-CoA synthetase; Provisional


Pssm-ID: 235673 [Multi-domain]  Cd Length: 542  Bit Score: 94.05  E-value: 5.03e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 268 AIFFTSGTSGYPKMTAHTHSSFGL-GLSVNGRFWLDLTPSDVMWNTSDTGWAkSAWSSVFSPWIQGACVFthhLP--RFE 344
Cdd:PRK06018  181 GMCYTSGTTGDPKGVLYSHRSNVLhALMANNGDALGTSAADTMLPVVPLFHA-NSWGIAFSAPSMGTKLV---MPgaKLD 256
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 345 PTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEPItPDVTEKWRNKTGLDIYEGYGQTETVLI-- 421
Cdd:PRK06018  257 GASVYELLDTEKVTFTAGVPTVWLMLLQYmEKEGLKLPHLKMVVCGGSAM-PRSMIKAFEDMGVEVRHAWGMTEMSPLgt 335
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 422 CGNFKG----------MKIKPgSMGKPSPAFDVKIVDVNGNVLPpgQEGdigiqvlpnRPFGLFThyVDNPSKTASTLRG 491
Cdd:PRK06018  336 LAALKPpfsklpgdarLDVLQ-KQGYPPFGVEMKITDDAGKELP--WDG---------KTFGRLK--VRGPAVAAAYYRV 401
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 492 N--------FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVL 563
Cdd:PRK06018  402 DgeildddgFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLIVQL 481
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1370468924 564 NPDyKSHDQEQLIKEIQEHVKKttapYKYPRKVEFIQELPKTISGKTKRNELRKK 618
Cdd:PRK06018  482 KPG-ETATREEILKYMDGKIAK----WWMPDDVAFVDAIPHTATGKILKTALREQ 531
PRK12467 PRK12467
peptide synthase; Provisional
269-615 5.59e-20

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 95.23  E-value: 5.59e-20
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  269 IFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSsVFSPWIQGACV----FTHHLprfE 344
Cdd:PRK12467  1723 VIYTSGSTGRPKGAGNRHGAL-VNRLCATQEAYQLSAADVVLQFTSFAFDVSVWE-LFWPLINGARLviapPGAHR---D 1797
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  345 PTSILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTG-LDIYEGYGQTETVL--- 420
Cdd:PRK12467  1798 PEQLIQLIERQQVTTLHFVPSMLQQLLQMDEQVEHPLSLRRVVCGGEALEVEALRPWLERLPdTGLFNLYGPTETAVdvt 1877
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  421 --IC--GNFKGMKIKPgsMGKPSPAFDVKIVDVNGNVLPPGQEGD--IGIQVLP----NRPFGLFTHYVDNPSktaSTLR 490
Cdd:PRK12467  1878 hwTCrrKDLEGRDSVP--IGQPIANLSTYILDASLNPVPIGVAGElyLGGVGLArgylNRPALTAERFVADPF---GTVG 1952
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  491 GNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSpDPIRGEVVKAFVV-LNPDYKS 569
Cdd:PRK12467  1953 SRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVIAQ-DGANGKQLVAYVVpTDPGLVD 2031
                          330       340       350       360
                   ....*....|....*....|....*....|....*....|....*...
gi 1370468924  570 HDQEQ--LIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:PRK12467  2032 DDEAQvaLRAILKNHLKASLPEYMVPAHLVFLARMPLTPNGKLDRKAL 2079
PRK12316 PRK12316
peptide synthase; Provisional
71-619 3.50e-19

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 92.71  E-value: 3.50e-19
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924   71 KEKAGKKPSNPAFwwinRNGEEmRWSFEELGSLSRKFANILSEaCSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPG 150
Cdd:PRK12316   518 EEQVERTPEAPAL----AFGEE-TLDYAELNRRANRLAHALIE-RGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPL 591
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  151 TTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVAskcenlhsklivsensregwgnlkELMKVFLCHPGwstvarSWLT 230
Cdd:PRK12316   592 DPEYPAERLAYMLEDSGVQLLLSQSHLGRKLPLAA------------------------GVQVLDLDRPA------AWLE 641
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  231 ATST-SLVQAIlsllsswdyrhasdshtcvktkHNEIMA-IFFTSGTSGYPKMTAHTHSSFglglsVNGRFW----LDLT 304
Cdd:PRK12316   642 GYSEeNPGTEL----------------------NPENLAyVIYTSGSTGKPKGAGNRHRAL-----SNRLCWmqqaYGLG 694
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  305 PSDVMWNTSDTGWAKSAWSsVFSPWIQGA-CVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSykFKS 382
Cdd:PRK12316   695 VGDTVLQKTPFSFDVSVWE-FFWPLMSGArLVVAAPGDHRDPAKLVELINREGVDTLHFVPSMLQAFLQDeDVAS--CTS 771
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  383 LKHCVSAGEPITPDVTEK-WRNKTGLDIYEGYGQTETVLICGNFKGMKIKPGS--MGKPSPAFDVKIVDVNGNVLPPGQE 459
Cdd:PRK12316   772 LRRIVCSGEALPADAQEQvFAKLPQAGLYNLYGPTEAAIDVTHWTCVEEGGDSvpIGRPIANLACYILDANLEPVPVGVL 851
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  460 GDIgiqVLPNRpfGLFTHYVDNPSKTASTLRGN-------FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVEN 532
Cdd:PRK12316   852 GEL---YLAGR--GLARGYHGRPGLTAERFVPSpfvagerMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEA 926
                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  533 ALNEHPSVAESAVVSspdpIRGEVVKAFVVLNPDYKShdqeqLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKR 612
Cdd:PRK12316   927 RLLEHPWVREAAVLA----VDGKQLVGYVVLESEGGD-----WREALKAHLAASLPEYMVPAQWLALERLPLTPNGKLDR 997

                   ....*..
gi 1370468924  613 NELRKKE 619
Cdd:PRK12316   998 KALPAPE 1004
PRK08308 PRK08308
acyl-CoA synthetase; Validated
335-619 4.25e-19

acyl-CoA synthetase; Validated


Pssm-ID: 236231 [Multi-domain]  Cd Length: 414  Bit Score: 90.10  E-value: 4.25e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 335 VFTHHLPRFeptsILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFKSLkhcVSAGEPITPDVTEKWRNKTgLDIYEGYG 414
Cdd:PRK08308  173 IITNKNPKF----ALNILRNTPQHILYAVPLMLHILGRLLPGTFQFHAV---MTSGTPLPEAWFYKLRERT-TYMMQQYG 244
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 415 QTET--VLICGNFKgmkiKPGSMGKPSPAFDVKIvdvngnvlppGQEgdigiqvlpnrpfglfthyVDNPSKTASTLRGN 492
Cdd:PRK08308  245 CSEAgcVSICPDMK----SHLDLGNPLPHVSVSA----------GSD-------------------ENAPEEIVVKMGDK 291
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 493 FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVlnpdykSHDQ 572
Cdd:PRK08308  292 EIFTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVI------SHEE 365
                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*..
gi 1370468924 573 EQLIkEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKE 619
Cdd:PRK08308  366 IDPV-QLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLLELGE 411
PRK07008 PRK07008
long-chain-fatty-acid--CoA ligase; Validated
178-616 4.58e-19

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235908 [Multi-domain]  Cd Length: 539  Bit Score: 90.92  E-value: 4.58e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 178 APAVDAVASKCENLhsklivsensrEGWgnlkelmkVFLCHpgwstvaRSWLTATSTSLvqailslLSSWDYRHA-SDSH 256
Cdd:PRK07008  122 LPLVDALAPQCPNV-----------KGW--------VAMTD-------AAHLPAGSTPL-------LCYETLVGAqDGDY 168
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 257 TCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSS-----FGLGLSVNgrfwLDLTPSDV------MWNTSdtgwaksAWSSV 325
Cdd:PRK07008  169 DWPRFDENQASSLCYTSGTTGNPKGALYSHRStvlhaYGAALPDA----MGLSARDAvlpvvpMFHVN-------AWGLP 237
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 326 FSPWIQGA-CVFTHhlPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCVSAGEPITPDVTEKWRN 403
Cdd:PRK07008  238 YSAPLTGAkLVLPG--PDLDGKSLYELIEAERVTFSAGVPTVWLGLLNHmREAGLRFSTLRRTVIGGSACPPAMIRTFED 315
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 404 KTGLDIYEGYGQTETVLIcGNFKGMKIKPGSM------------GKPSPAFDVKIVDVNGNVLP-PGQE-GDIgiQVlpn 469
Cdd:PRK07008  316 EYGVEVIHAWGMTEMSPL-GTLCKLKWKHSQLpldeqrkllekqGRVIYGVDMKIVGDDGRELPwDGKAfGDL--QV--- 389
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 470 RPFGLFTHYVDNpskTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSP 549
Cdd:PRK07008  390 RGPWVIDRYFRG---DASPLVDGWFPTGDVATIDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACA 466
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1370468924 550 DPIRGEVVKAFVVLNPDYKShDQEQLIKeiqeHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PRK07008  467 HPKWDERPLLVVVKRPGAEV-TREELLA----FYEGKVAKWWIPDDVVFVDAIPHTATGKLQKLKLR 528
PRK12316 PRK12316
peptide synthase; Provisional
239-617 6.18e-19

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 91.94  E-value: 6.18e-19
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  239 AILSLLSSWDYRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWlDLTPSDVMWNTSDTGWA 318
Cdd:PRK12316  2121 ARLPLDRDAEWADYPDTAPAVQLAGENLAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGERY-ELSPADCELQFMSFSFD 2199
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  319 KSAWSsVFSPWIQGACVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSYKFKSLKHCVSAGEPITPDVT 398
Cdd:PRK12316  2200 GAHEQ-WFHPLLNGARVLIRDDELWDPEQLYDEMERHGVTILDFPPVYLQQLAEHAERDGRPPAVRVYCFGGEAVPAASL 2278
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  399 EK-WRNKTGLDIYEGYGQTETVLICGNFKGMKIKPGS-----MGKPSPAFDVKIVDVNGNVLPPGQEGD--IGIQvlpnr 470
Cdd:PRK12316  2279 RLaWEALRPVYLFNGYGPTEAVVTPLLWKCRPQDPCGaayvpIGRALGNRRAYILDADLNLLAPGMAGElyLGGE----- 2353
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  471 pfGLFTHYVDNPSKTA--------STLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAE 542
Cdd:PRK12316  2354 --GLARGYLNRPGLTAerfvpdpfSASGERLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVRE 2431
                          330       340       350       360       370       380       390
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1370468924  543 SAVVSSpDPIRGEVVKAFVVlnPDyksHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK12316  2432 AVVVAQ-DGASGKQLVAYVV--PD---DAAEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPK 2500
A_NRPS_ProA cd17656
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ...
251-615 1.35e-18

gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341311 [Multi-domain]  Cd Length: 479  Bit Score: 89.07  E-value: 1.35e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 251 HASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSDVMWNTSDTgwAKSAWSSVFSPWI 330
Cdd:cd17656   115 QEDTSNIDYINNSDDLLYIIYTSGTTGKPKGVQLEHKNMVNLLHFEREKTNINFSDKVLQFATCS--FDVCYQEIFSTLL 192
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 331 QGAcvfTHHLPRFEPTSILQTL----SKYPITVFcSAPTVYRMLVQNDITSYK--FKSLKHCVSAGEP--ITPDVTEKWR 402
Cdd:cd17656   193 SGG---TLYIIREETKRDVEQLfdlvKRHNIEVV-FLPVAFLKFIFSEREFINrfPTCVKHIITAGEQlvITNEFKEMLH 268
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 403 NKtGLDIYEGYGQTETVLIcgnfKGMKIKPGS-------MGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVLpnrpfGLF 475
Cdd:cd17656   269 EH-NVHLHNHYGPSETHVV----TTYTINPEAeipelppIGKPISNTWIYILDQEQQLQPQGIVGELYISGA-----SVA 338
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 476 THYVDNPSKTASTLRGN-------FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSS 548
Cdd:cd17656   339 RGYLNRQELTAEKFFPDpfdpnerMYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDK 418
                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1370468924 549 PDPIRGEVVKAFVVLnpdykshDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd17656   419 ADDKGEKYLCAYFVM-------EQELNISQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
PRK08279 PRK08279
long-chain-acyl-CoA synthetase; Validated
63-617 2.96e-18

long-chain-acyl-CoA synthetase; Validated


Pssm-ID: 236217 [Multi-domain]  Cd Length: 600  Bit Score: 88.39  E-value: 2.96e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  63 DVLDQWTDKekagkKPSNPAFwwinrNGEEMRWSFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLR 142
Cdd:PRK08279   41 DVFEEAAAR-----HPDRPAL-----LFEDQSISYAELNARANRYAHWAAAR-GVGKGDVVALLMENRPEYLAAWLGLAK 109
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 143 TGTV--LIpgTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASkCENLHSKLIV----SENSREGWGNLKELMKVFl 216
Cdd:PRK08279  110 LGAVvaLL--NTQQRGAVLAHSLNLVDAKHLIVGEELVEAFEEARA-DLARPPRLWVaggdTLDDPEGYEDLAAAAAGA- 185
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 217 chPGWSTVARSWLTATSTSLvqailsllsswdYrhasdshtcvktkhneimaiFFTSGTSGYPKMTAHTH-----SSFGL 291
Cdd:PRK08279  186 --PTTNPASRSGVTAKDTAF------------Y--------------------IYTSGTTGLPKAAVMSHmrwlkAMGGF 231
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 292 GLSvngrfwLDLTPSDVMWNT----SDTGwAKSAWSSVFSPwiqGACV-----FThhLPRFEPTSIlqtlsKYPITVFCS 362
Cdd:PRK08279  232 GGL------LRLTPDDVLYCClplyHNTG-GTVAWSSVLAA---GATLalrrkFS--ASRFWDDVR-----RYRATAFQY 294
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 363 APTVYRMLVQNDITSY-KFKSLKHCVSAGepITPDVTEKWRNKTGLD-IYEGYGQTE--TVLIcgNFKGmkiKPGSMGKp 438
Cdd:PRK08279  295 IGELCRYLLNQPPKPTdRDHRLRLMIGNG--LRPDIWDEFQQRFGIPrILEFYAASEgnVGFI--NVFN---FDGTVGR- 366
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 439 SPAFDVK---IV-----------DVNGNVLP--PGQEGD-IGiQVLPNRPFglfTHYVDnPSKT-ASTLRGNF------Y 494
Cdd:PRK08279  367 VPLWLAHpyaIVkydvdtgepvrDADGRCIKvkPGEVGLlIG-RITDRGPF---DGYTD-PEASeKKILRDVFkkgdawF 441
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 495 ITGDRGYMDKDGYFWFVARADDVilssgYR-----IGPFEVENALNEHPSVAESAV--VSSPDpIRGEVVKAFVVLnpdy 567
Cdd:PRK08279  442 NTGDLMRDDGFGHAQFVDRLGDT-----FRwkgenVATTEVENALSGFPGVEEAVVygVEVPG-TDGRAGMAAIVL---- 511
                         570       580       590       600       610
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1370468924 568 ksHDQEQL-IKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK08279  512 --ADGAEFdLAALAAHLYERLPAYAVPLFVRLVPELETTGTFKYRKVDLRK 560
FAAL cd05931
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ...
74-617 7.11e-17

Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.


Pssm-ID: 341254 [Multi-domain]  Cd Length: 547  Bit Score: 83.83  E-value: 7.11e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  74 AGKKPSNPAFWWINR-NGEEMRWSFEELGSLSRKFANILSEACslQRGDRVILILPRVPEWWLANVACLRTGTV---LIP 149
Cdd:cd05931     3 AAARPDRPAYTFLDDeGGREETLTYAELDRRARAIAARLQAVG--KPGDRVLLLAPPGLDFVAAFLGCLYAGAIavpLPP 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 150 GTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKcenlhsklivsensregwgnlkelmkvflcHPGWSTVARSWL 229
Cdd:cd05931    81 PTPGRHAERLAAILADAGPRVVLTTAAALAAVRAFAAS------------------------------RPAAGTPRLLVV 130
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 230 TATSTSlvqailsllsswdyrhASDSHTCVKTKHNEIMAIFFTSGTSGYPK--MTAHT--HSSFGLGLSVngrfwLDLTP 305
Cdd:cd05931   131 DLLPDT----------------SAADWPPPSPDPDDIAYLQYTSGSTGTPKgvVVTHRnlLANVRQIRRA-----YGLDP 189
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 306 SDVMWNtsdtgwaksaW----------SSVFSPWIQGA-CVFTHhlPR-F--EPTSILQTLSKYPITvFCSAPT-VYRML 370
Cdd:cd05931   190 GDVVVS----------WlplyhdmgliGGLLTPLYSGGpSVLMS--PAaFlrRPLRWLRLISRYRAT-ISAAPNfAYDLC 256
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 371 VQ----NDITSYKFKSLKHCVSAGEPITPDVTEKWRNK---TGLD---IYEGYGQTE-TVLICGNFKG------------ 427
Cdd:cd05931   257 VRrvrdEDLEGLDLSSWRVALNGAEPVRPATLRRFAEAfapFGFRpeaFRPSYGLAEaTLFVSGGPPGtgpvvlrvdrda 336
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 428 ------MKIKPG-------SMGKPSPAFDVKIVDVNGN-VLPPGQEGDIGIQVlPNRPFGlfthYVDNPSKTASTLR--- 490
Cdd:cd05931   337 lagravAVAADDpaarelvSCGRPLPDQEVRIVDPETGrELPDGEVGEIWVRG-PSVASG----YWGRPEATAETFGala 411
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 491 ----GNFYITGDRGYMdKDGYFWFVARADDVILSSGYRIGPFEVENAL-NEHPSVAES--AVVSSPDPIRGEVVkAFVVL 563
Cdd:cd05931   412 atdeGGWLRTGDLGFL-HDGELYITGRLKDLIIVRGRNHYPQDIEATAeEAHPALRPGcvAAFSVPDDGEERLV-VVAEV 489
                         570       580       590       600       610       620
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1370468924 564 NPDYKSHDQEQLIKEIQ-----EH-VKkttapykyPRKVEFI--QELPKTISGKTKRNELRK 617
Cdd:cd05931   490 ERGADPADLAAIAAAIRaavarEHgVA--------PADVVLVrpGSIPRTSSGKIQRRACRA 543
LC-FACS_euk cd05927
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ...
262-623 9.86e-17

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.


Pssm-ID: 341250 [Multi-domain]  Cd Length: 545  Bit Score: 83.42  E-value: 9.86e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 262 KHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLtpsdVMWNTSDTGWAKSAWSSVFSPWIQ------GACV 335
Cdd:cd05927   112 KPEDLATICYTSGTTGNPKGVMLTHGNIVSNVAGVFKILEIL----NKINPTDVYISYLPLAHIFERVVEalflyhGAKI 187
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 336 -FTHHLPRfEPTSILQTLSkyPiTVFCSAPTVY-RML--VQNDI--------------TSYKFKSLKH------------ 385
Cdd:cd05927   188 gFYSGDIR-LLLDDIKALK--P-TVFPGVPRVLnRIYdkIFNKVqakgplkrklfnfaLNYKLAELRSgvvraspfwdkl 263
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 386 ---------------CVSAGEPITPDVTEKWRNKTGLDIYEGYGQTETV-LICGNFKGMKIkPGSMGKPSPAFDVKIVDV 449
Cdd:cd05927   264 vfnkikqalggnvrlMLTGSAPLSPEVLEFLRVALGCPVLEGYGQTECTaGATLTLPGDTS-VGHVGGPLPCAEVKLVDV 342
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 450 ---NGNVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGN-FYITGDRGYMDKDGYFWFVARADDVI-LSSGYR 524
Cdd:cd05927   343 pemNYDAKDPNPRGEVCI-----RGPNVFSGYYKDPEKTAEALDEDgWLHTGDIGEWLPNGTLKIIDRKKNIFkLSQGEY 417
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 525 IGPFEVENALNEHPSVAESAVvsspdpiRGEVVKAF----VVLNPDY------------KSHDQ----EQLIKEIQEHVK 584
Cdd:cd05927   418 VAPEKIENIYARSPFVAQIFV-------YGDSLKSFlvaiVVPDPDVlkewaaskgggtGSFEElcknPEVKKAILEDLV 490
                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1370468924 585 KTTAPYKYpRKVEFIQEL---PK---------TISGKTKRNELRKKEWKTI 623
Cdd:cd05927   491 RLGKENGL-KGFEQVKAIhlePEpfsvengllTPTFKLKRPQLKKYYKKQI 540
A_NRPS_PpsD_like cd17650
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ...
269-615 2.04e-16

similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341305 [Multi-domain]  Cd Length: 447  Bit Score: 82.13  E-value: 2.04e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 IFFTSGTSGYPKMTAHTHSSFG-LGLSVNGRFWLDLTPSDVMWNTS---DTGWAKSAWSSVFSpwiqGACVFTHHLPRFE 344
Cdd:cd17650    98 VIYTSGTTGKPKGVMVEHRNVAhAAHAWRREYELDSFPVRLLQMASfsfDVFAGDFARSLLNG----GTLVICPDEVKLD 173
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 345 PTSILQTLSKYPITVFCSAPTVYRMLVQN-DITSYKFKSLKHCV--SAGEPITPDVTEKWRNKTGLDIYEGYGQTETVLI 421
Cdd:cd17650   174 PAALYDLILKSRITLMESTPALIRPVMAYvYRNGLDLSAMRLLIvgSDGCKAQDFKTLAARFGQGMRIINSYGVTEATID 253
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 422 CGNFK-GMKIKPGS----MGKPSPAFDVKIVDVNGNVLPPGQEGDIGI------QVLPNRPFGLFTHYVDNPSKTAstlr 490
Cdd:cd17650   254 STYYEeGRDPLGDSanvpIGRPLPNTAMYVLDERLQPQPVGVAGELYIggagvaRGYLNRPELTAERFVENPFAPG---- 329
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 491 GNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPiRGEV-VKAFVVlnPDYKS 569
Cdd:cd17650   330 ERMYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVAVREDK-GGEArLCAYVV--AAATL 406
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*.
gi 1370468924 570 HdqeqlIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd17650   407 N-----TAELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
A_NRPS_acs4 cd17654
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ...
229-615 3.01e-16

acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341309 [Multi-domain]  Cd Length: 449  Bit Score: 81.36  E-value: 3.01e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 229 LTATSTSLVQAILS---LLSSWDYRHASDSHTCVKTKHNEIMAIFfTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTP 305
Cdd:cd17654    81 LTVMKKCHVSYLLQnkeLDNAPLSFTPEHRHFNIRTDECLAYVIH-TSGTTGTPKIVAVPHKCI-LPNIQHFRSLFNITS 158
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 306 SDVMWNTSDTGWAKSAwSSVFSPWIQGAC-VFTHHLPRFEPTSILQTLSKYP-ITVFCSAPTVYRMLVQNDITSY---KF 380
Cdd:cd17654   159 EDILFLTSPLTFDPSV-VEIFLSLSSGATlLIVPTSVKVLPSKLADILFKRHrITVLQATPTLFRRFGSQSIKSTvlsAT 237
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 381 KSLKHCVSAGEPITPDVTEK-WRNK-TGLDIYEGYGQTETVlICGNFKGMKIKPGSMGKPSPAFDVKI--VDVNGNVlPP 456
Cdd:cd17654   238 SSLRVLALGGEPFPSLVILSsWRGKgNRTRIFNIYGITEVS-CWALAYKVPEEDSPVQLGSPLLGTVIevRDQNGSE-GT 315
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 457 GQEGDIGIqvlpNRpFGLFTHYVDNPsktastlRGNFYITGDRGYMdKDGYFWFVARADDVILSSGYRIGPFEVENALNE 536
Cdd:cd17654   316 GQVFLGGL----NR-VCILDDEVTVP-------KGTMRATGDFVTV-KDGELFFLGRKDSQIKRRGKRINLDLIQQVIES 382
                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1370468924 537 HPSVAESAVVSSPDpirgEVVKAFVVLNPdykSHDQEQliKEIQEHVKKTTApykYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:cd17654   383 CLGVESCAVTLSDQ----QRLIAFIVGES---SSSRIH--KELQLTLLSSHA---IPDTFVQIDKLPLTSHGKVDKSEL 449
entF PRK10252
enterobactin non-ribosomal peptide synthetase EntF;
96-619 3.54e-16

enterobactin non-ribosomal peptide synthetase EntF;


Pssm-ID: 236668 [Multi-domain]  Cd Length: 1296  Bit Score: 82.78  E-value: 3.54e-16
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924   96 SFEELGSLSRKFANILSEAcSLQRGDRVILILPRVPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITND 175
Cdd:PRK10252   485 SYREMREQVVALANLLRER-GVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTA 563
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  176 VLAPAVDAVASKcenlhsklivsensregwgnlkelmkVFLCHPGWstvarsWLTATSTSLVqaiLSLLSSWDYrhasds 255
Cdd:PRK10252   564 DQLPRFADVPDL--------------------------TSLCYNAP------LAPQGAAPLQ---LSQPHHTAY------ 602
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  256 htcvktkhneimaIFFTSGTSGYPKMTAHTHSSFglglsVNGRFWLD----LTPSDVMWNTSDTGWAKSAWSsVFSPWIQ 331
Cdd:PRK10252   603 -------------IIFTSGSTGRPKGVMVGQTAI-----VNRLLWMQnhypLTADDVVLQKTPCSFDVSVWE-FFWPFIA 663
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  332 GACVF-----THHlprfEPTSILQTLSKYPITV----------FCSAPTVyrmlvqnDITSYKFKSLKHCVSAGEPITPD 396
Cdd:PRK10252   664 GAKLVmaepeAHR----DPLAMQQFFAEYGVTTthfvpsmlaaFVASLTP-------EGARQSCASLRQVFCSGEALPAD 732
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  397 VTEKWRNKTGLDIYEGYGQTET-VLICGNFKGMKIKPGSMGKPSP-AFDV-----KIVDVNGNVLPPGQEGDI---GIQv 466
Cdd:PRK10252   733 LCREWQQLTGAPLHNLYGPTEAaVDVSWYPAFGEELAAVRGSSVPiGYPVwntglRILDARMRPVPPGVAGDLyltGIQ- 811
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  467 lpnrpfgLFTHYVDNPSKTASTLRGN-------FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPS 539
Cdd:PRK10252   812 -------LAQGYLGRPDLTASRFIADpfapgerMYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQALPD 884
                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  540 VAESA----VVSSPDPIRGEVVK--AFVVLNPDYkSHDQEQLikeiQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRN 613
Cdd:PRK10252   885 VEQAVthacVINQAAATGGDARQlvGYLVSQSGL-PLDTSAL----QAQLRERLPPHMVPVVLLQLDQLPLSANGKLDRK 959

                   ....*.
gi 1370468924  614 ELRKKE 619
Cdd:PRK10252   960 ALPLPE 965
FACL_like_1 cd05910
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
261-546 3.92e-16

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341236 [Multi-domain]  Cd Length: 457  Bit Score: 81.35  E-value: 3.92e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 261 TKHNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNgRFWLDLTPSDVMWntsdtgwAKSAWSSVFSPWIQGACV---FT 337
Cdd:cd05910    82 PKADEPAAILFTSGSTGTPKGVVYRHGTFAAQIDAL-RQLYGIRPGEVDL-------ATFPLFALFGPALGLTSVipdMD 153
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 338 HHLP-RFEPTSILQTLSKYPITVFCSAPTVYRML----VQNDITsykFKSLKHCVSAGEPITPDVTEKWRN--KTGLDIY 410
Cdd:cd05910   154 PTRPaRADPQKLVGAIRQYGVSIVFGSPALLERVarycAQHGIT---LPSLRRVLSAGAPVPIALAARLRKmlSDEAEIL 230
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 411 EGYGQTETVLICG-------NFKGMKIKPGS---MGKPSPAFDVKIVDVN---------GNVLPPGQEGDI---GIQVLP 468
Cdd:cd05910   231 TPYGATEALPVSSigsrellATTTAATSGGAgtcVGRPIPGVRVRIIEIDdepiaewddTLELPRGEIGEItvtGPTVTP 310
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 469 NrpfglfthYVDNPSKTA----STLRGNF-YITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAES 543
Cdd:cd05910   311 T--------YVNRPVATAlakiDDNSEGFwHRMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVRRS 382

                  ...
gi 1370468924 544 AVV 546
Cdd:cd05910   383 ALV 385
A_NRPS_MycA_like cd05908
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ...
264-618 5.78e-15

The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341234 [Multi-domain]  Cd Length: 499  Bit Score: 77.91  E-value: 5.78e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 264 NEIMAIFFTSGTSGYPKMTAHTHSSFGLG-LSVNGRFWLDLTPSDVMWN--TSDTGWAksawSSVFSPWIQGACVFTHHL 340
Cdd:cd05908   106 DELAFIQFSSGSTGDPKGVMLTHENLVHNmFAILNSTEWKTKDRILSWMplTHDMGLI----AFHLAPLIAGMNQYLMPT 181
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 341 PRF--EPTSILQTLSKYPITVFCSAPTVYRMLVQ----NDITSYKFKSLKHCVSAGEPITPDVTE---KWRNKTGLD--- 408
Cdd:cd05908   182 RLFirRPILWLKKASEHKATIVSSPNFGYKYFLKtlkpEKANDWDLSSIRMILNGAEPIDYELCHeflDHMSKYGLKrna 261
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 409 IYEGYGQTE-TVLICGNFKGMKIKPGSM-------GKPSPAFD--------------------VKIVDVNGNVLPPGQEG 460
Cdd:cd05908   262 ILPVYGLAEaSVGASLPKAQSPFKTITLgrrhvthGEPEPEVDkkdsecltfvevgkpidetdIRICDEDNKILPDGYIG 341
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 461 DI---GIQVLPNrpfglfthYVDNPSKTASTLRGN-FYITGDRGYMdKDGYFWFVARADDVILSSGYRIGPFEVENALNE 536
Cdd:cd05908   342 HIqirGKNVTPG--------YYNNPEATAKVFTDDgWLKTGDLGFI-RNGRLVITGREKDIIFVNGQNVYPHDIERIAEE 412
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 537 HPSVAESAVVS---SPDPIRGEVVKAFVVLNpdyKSHDQ-EQLIKEIQEHVKKTTApyKYPRKVEFIQELPKTISGKTKR 612
Cdd:cd05908   413 LEGVELGRVVAcgvNNSNTRNEEIFCFIEHR---KSEDDfYPLGKKIKKHLNKRGG--WQINEVLPIRRIPKTTSGKVKR 487

                  ....*.
gi 1370468924 613 NELRKK 618
Cdd:cd05908   488 YELAQR 493
PRK06814 PRK06814
acyl-[ACP]--phospholipid O-acyltransferase;
268-617 1.55e-14

acyl-[ACP]--phospholipid O-acyltransferase;


Pssm-ID: 235865 [Multi-domain]  Cd Length: 1140  Bit Score: 77.31  E-value: 1.55e-14
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  268 AIFFTSGTSGYPKMTAHTHSSFglgLS----VNGRfwLDLTPSDVMWNTsdtgwaksawssvfspwiqgacvfthhLPRF 343
Cdd:PRK06814   797 VILFTSGSEGTPKGVVLSHRNL---LAnraqVAAR--IDFSPEDKVFNA---------------------------LPVF 844
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  344 EP---TS--ILQTLSKYPITVFCSaPTVYR----MLVQNDIT-----------------SYKFKSLKHCVSAGEPITPDV 397
Cdd:PRK06814   845 HSfglTGglVLPLLSGVKVFLYPS-PLHYRiipeLIYDTNATilfgtdtflngyaryahPYDFRSLRYVFAGAEKVKEET 923
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  398 TEKWRNKTGLDIYEGYGQTET--VLICGNfkGMKIKPGSMGKPSPAFDVKIVDVngnvlpPGQEGDIGIQVL-PNRPFGl 474
Cdd:PRK06814   924 RQTWMEKFGIRILEGYGVTETapVIALNT--PMHNKAGTVGRLLPGIEYRLEPV------PGIDEGGRLFVRgPNVMLG- 994
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  475 fthYV--DNPSkTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPI 552
Cdd:PRK06814   995 ---YLraENPG-VLEPPADGWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAAELWPDALHAAVSIPDAR 1070
                          330       340       350       360       370       380
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1370468924  553 RGEVvkafVVLNPDYKSHDQEqlikEIQEHVKKTTAPYKY-PRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK06814  1071 KGER----IILLTTASDATRA----AFLAHAKAAGASELMvPAEIITIDEIPLLGTGKIDYVAVTK 1128
PRK08043 PRK08043
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
259-617 2.59e-14

bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;


Pssm-ID: 181207 [Multi-domain]  Cd Length: 718  Bit Score: 76.29  E-value: 2.59e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 259 VKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTh 338
Cdd:PRK08043  360 VKQQPEDAALILFTSGSEGHPKGVVHSHKSL-LANVEQIKTIADFTPNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFL- 437
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 339 hlprfeptsilqtlskYPitvfcsAPTVYRM---LV--QNDI----TS------------YKFKSLKHCVSAGEPITPDV 397
Cdd:PRK08043  438 ----------------YP------SPLHYRIvpeLVydRNCTvlfgTStflgnyarfanpYDFARLRYVVAGAEKLQEST 495
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 398 TEKWRNKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVngnvlpPGQEGDIGIQVL-PNRPFGLFT 476
Cdd:PRK08043  496 KQLWQDKFGLRILEGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSV------PGIEQGGRLQLKgPNIMNGYLR 569
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 477 hyVDNPSK----TASTLRG----NFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSS 548
Cdd:PRK08043  570 --VEKPGVlevpTAENARGemerGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDKQHATAIK 647
                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1370468924 549 PDPIRGEvvkAFVVLNPDyKSHDQEQLIKEIQEHVKKTTApykYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK08043  648 SDASKGE---ALVLFTTD-SELTREKLQQYAREHGVPELA---VPRDIRYLKQLPLLGSGKPDFVTLKS 709
FATP_FACS cd05940
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ...
271-617 6.77e-14

Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341263 [Multi-domain]  Cd Length: 449  Bit Score: 74.31  E-value: 6.77e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 271 FTSGTSGYPKMTAHTHSSFGLGLSVNGrFWLDLTPSDVMWNT----SDTGwAKSAWSSVFspwIQGACVFTHHlpRFEPT 346
Cdd:cd05940    88 YTSGTTGLPKAAIISHRRAWRGGAFFA-GSGGALPSDVLYTClplyHSTA-LIVGWSACL---ASGATLVIRK--KFSAS 160
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 347 SILQTLSKYPITVFCSAPTVYRMLVQndiTSYKFKSLKHCVSA--GEPITPDVTEKWRNKTGL-DIYEGYGQTETVLICG 423
Cdd:cd05940   161 NFWDDIRKYQATIFQYIGELCRYLLN---QPPKPTERKHKVRMifGNGLRPDIWEEFKERFGVpRIAEFYAATEGNSGFI 237
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 424 NFKGmkiKPGSMGKPSP----AFDVKIV-----------DVNGNV--LPPGQEGDIGIQVLPNRPFglfTHYVDNPSKTA 486
Cdd:cd05940   238 NFFG---KPGAIGRNPSllrkVAPLALVkydlesgepirDAEGRCikVPRGEPGLLISRINPLEPF---DGYTDPAATEK 311
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 487 STLRGNF------YITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAV--VSSPDpIRGEVVK 558
Cdd:cd05940   312 KILRDVFkkgdawFNTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVygVQVPG-TDGRAGM 390
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1370468924 559 AFVVLNPDYkSHDQEQLikeiQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05940   391 AAIVLQPNE-EFDLSAL----AAHLEKNLPGYARPLFLRLQPEMEITGTFKQQKVDLRN 444
PRK05691 PRK05691
peptide synthase; Validated
271-615 2.47e-13

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 73.66  E-value: 2.47e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  271 FTSGTSGYPKMTAHTHSSFGLGL-SVNGRFwlDLTPSDVMWNTSDTGWaKSAWSSVFSPWIQGACVFTHHLPRFEPTSIL 349
Cdd:PRK05691  2340 YTSGSTGKPKGVVVSHGEIAMHCqAVIERF--GMRADDCELHFYSINF-DAASERLLVPLLCGARVVLRAQGQWGAEEIC 2416
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  350 QTLSKYPITVFCSAPTVYRMLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRNKTGLD-IYEGYGQTETV---LICGNF 425
Cdd:PRK05691  2417 QLIREQQVSILGFTPSYGSQLAQWLAGQGEQLPVRMCITGGEALTGEHLQRIRQAFAPQlFFNAYGPTETVvmpLACLAP 2496
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  426 KGMKIKPGS--MGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVLpnrpfGLFTHYVDNPSKTA--------STLRGNFYI 495
Cdd:PRK05691  2497 EQLEEGAASvpIGRVVGARVAYILDADLALVPQGATGELYVGGA-----GLAQGYHDRPGLTAerfvadpfAADGGRLYR 2571
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  496 TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYKSHDQEQL 575
Cdd:PRK05691  2572 TGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVREAVVLALDTPSGKQLAGYLVSAVAGQDDEAQAAL 2651
                          330       340       350       360
                   ....*....|....*....|....*....|....*....|
gi 1370468924  576 IKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:PRK05691  2652 REALKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRAL 2691
PRK05691 PRK05691
peptide synthase; Validated
269-615 4.15e-13

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 72.89  E-value: 4.15e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  269 IFFTSGTSGYPKmtahthssfglGLSVNGRF----------WLDLTPSDVMWNTSDTGWAKSAWSSVFSPWIQGACVFTH 338
Cdd:PRK05691  3874 VIYTSGSTGLPK-----------GVMVEQRGmlnnqlskvpYLALSEADVIAQTASQSFDISVWQFLAAPLFGARVEIVP 3942
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  339 HLPRFEPTSILQTLSKYPITVFCSAPT-VYRMLVQNDITsykFKSLKHCVSAGEPITPDVTEKWRNK-TGLDIYEGYGQT 416
Cdd:PRK05691  3943 NAIAHDPQGLLAHVQAQGITVLESVPSlIQGMLAEDRQA---LDGLRWMLPTGEAMPPELARQWLQRyPQIGLVNAYGPA 4019
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  417 ETVLICGNFK-GMKIKPGS---MGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQvlpnrPFGLFTHYVDNPSKTASTLRGN 492
Cdd:PRK05691  4020 ECSDDVAFFRvDLASTRGSylpIGSPTDNNRLYLLDEALELVPLGAVGELCVA-----GTGVGRGYVGDPLRTALAFVPH 4094
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  493 --------FYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPiRGEVVKAFVVln 564
Cdd:PRK05691  4095 pfgapgerLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAAVAVQEGV-NGKHLVGYLV-- 4171
                          330       340       350       360       370
                   ....*....|....*....|....*....|....*....|....*....|.
gi 1370468924  565 PDYKSHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNEL 615
Cdd:PRK05691  4172 PHQTVLAQGALLERIKQRLRAELPDYMVPLHWLWLDRLPLNANGKLDRKAL 4222
PLN03051 PLN03051
acyl-activating enzyme; Provisional
268-616 7.68e-13

acyl-activating enzyme; Provisional


Pssm-ID: 215552 [Multi-domain]  Cd Length: 499  Bit Score: 71.00  E-value: 7.68e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 268 AIFFTSGTSGYPKMTAHTHSSfGLGLSVNGRFWLDLTPSDVM-WNTSdTGWAKSAWSsVFSPWIQGACVFTHHlprFEPT 346
Cdd:PLN03051  123 NILFSSGTTGEPKAIPWTHLS-PLRCASDGWAHMDIQPGDVVcWPTN-LGWMMGPWL-LYSAFLNGATLALYG---GAPL 196
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 347 S--ILQTLSKYPITVFCSAPTV---YRMLVQNDITSYKFKSLKHCVSAGEPITPDvTEKWRNKT------------GLDI 409
Cdd:PLN03051  197 GrgFGKFVQDAGVTVLGLVPSIvkaWRHTGAFAMEGLDWSKLRVFASTGEASAVD-DVLWLSSVrgyykpvieycgGTEL 275
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 410 YEGYGQTETVLICGnfkgmkikPGSMGKPSPAFDVKIVDVNGNVLPPGQE--GDIGI--------QVLPNRPFGLfTHYV 479
Cdd:PLN03051  276 ASGYISSTLLQPQA--------PGAFSTASLGTRFVLLNDNGVPYPDDQPcvGEVALappmlgasDRLLNADHDK-VYYK 346
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 480 DNP--SKTASTLRGNfyitGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNE-HPSVAESAVVSSPDPIRGE- 555
Cdd:PLN03051  347 GMPmyGSKGMPLRRH----GDIMKRTPGGYFCVQGRADDTMNLGGIKTSSVEIERACDRaVAGIAETAAVGVAPPDGGPe 422
                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1370468924 556 ---VVKAFVVLNPDYKSHDQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELR 616
Cdd:PLN03051  423 llvIFLVLGEEKKGFDQARPEALQKKFQEAIQTNLNPLFKVSRVKIVPELPRNASNKLLRRVLR 486
PRK05691 PRK05691
peptide synthase; Validated
263-622 7.82e-13

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 72.12  E-value: 7.82e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  263 HNEIMA-IFFTSGTSGYPKMTAHTHSSFGLGLSvngrfWLD----LTPSDVMWNTSDTGWAKSAWSsVFSPWIQG----- 332
Cdd:PRK05691  1271 HGDNLAyVIYTSGSTGQPKGVGNTHAALAERLQ-----WMQatyaLDDSDVLMQKAPISFDVSVWE-CFWPLITGcrlvl 1344
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  333 ACVFTHHlprfEPTSILQTLSKYPITVFCSAPTVYRMLVQNDITSyKFKSLKHCVSAGEPITPDVTEKWRNK-TGLDIYE 411
Cdd:PRK05691  1345 AGPGEHR----DPQRIAELVQQYGVTTLHFVPPLLQLFIDEPLAA-ACTSLRRLFSGGEALPAELRNRVLQRlPQVQLHN 1419
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  412 GYGQTETVL-----ICGNFKGMKikpGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVLpnrpfGLFTHYVDNPSKTA 486
Cdd:PRK05691  1420 RYGPTETAInvthwQCQAEDGER---SPIGRPLGNVLCRVLDAELNLLPPGVAGELCIGGA-----GLARGYLGRPALTA 1491
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  487 --------STLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVsspdpIRGEVVK 558
Cdd:PRK05691  1492 erfvpdplGEDGARLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEPEEIQARLLAQPGVAQAAVL-----VREGAAG 1566
                          330       340       350       360       370       380
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1370468924  559 AFVVlnpDYKSHDQEQliKEIQEHVKKTTA----PYKYPRKVEFIQELPKTISGKTKRNELRKKEWKT 622
Cdd:PRK05691  1567 AQLV---GYYTGEAGQ--EAEAERLKAALAaelpEYMVPAQLIRLDQMPLGPSGKLDRRALPEPVWQQ 1629
PRK07824 PRK07824
o-succinylbenzoate--CoA ligase;
494-617 1.01e-12

o-succinylbenzoate--CoA ligase;


Pssm-ID: 236108 [Multi-domain]  Cd Length: 358  Bit Score: 69.69  E-value: 1.01e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 494 YITGDRGYMDkDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVlnpdyKSHDQE 573
Cdd:PRK07824  236 FRTDDLGALD-DGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAVV-----GDGGPA 309
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1370468924 574 QLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:PRK07824  310 PTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALVR 353
hsFATP4_like cd05939
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ...
94-617 1.03e-12

Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341262 [Multi-domain]  Cd Length: 474  Bit Score: 70.53  E-value: 1.03e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  94 RWSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEW---WLAnVACLRTGTVLIpgTTQLTQKDILYRLQSSKANC 170
Cdd:cd05939     3 HWTFRELNEYSNKVANFFQ-AQGYRSGDVVALFMENRLEFvalWLG-LAKIGVETALI--NSNLRLESLLHCITVSKAKA 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 171 IITNdvlapavdavaskcenLHSKLivsensregwgnlkelmkvflchpgwstvarswLTATSTSLvqailsllsswdyr 250
Cdd:cd05939    79 LIFN----------------LLDPL---------------------------------LTQSSTEP-------------- 95
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 251 hasdsHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSFgLGLSVNGRFWLDLTPSDVMWNTSDTGWAKSAWSSVFSPWI 330
Cdd:cd05939    96 -----PSQDDVNFRDKLFYIYTSGTTGLPKAAVIVHSRY-YRIAAGAYYAFGMRPEDVVYDCLPLYHSAGGIMGVGQALL 169
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 331 QGACVFTHHlpRFEPTSILQTLSKYPITVFCSAPTVYRMLVQndiTSYKFKSLKHCV--SAGEPITPDVTEKWRNKTGL- 407
Cdd:cd05939   170 HGSTVVIRK--KFSASNFWDDCVKYNCTIVQYIGEICRYLLA---QPPSEEEQKHNVrlAVGNGLRPQIWEQFVRRFGIp 244
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 408 DIYEGYGQTETVLICGNFKG------------MKIKPGSMGKPSPAFDVKIVDVNGNVLP--PGQEGDIGIQVLPNRPFG 473
Cdd:cd05939   245 QIGEFYGATEGNSSLVNIDNhvgacgfnsrilPSVYPIRLIKVDEDTGELIRDSDGLCIPcqPGEPGLLVGKIIQNDPLR 324
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 474 LFTHYVDNPSKTASTLRGNF------YITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAV-- 545
Cdd:cd05939   325 RFDGYVNEGATNKKIARDVFkkgdsaFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVyg 404
                         490       500       510       520       530       540       550
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1370468924 546 VSSPDpIRGEVVKAFVVlNPDYKShDQEQLIKEIQehvkKTTAPYKYPRKVEFIQELPKTISGKTKRNELRK 617
Cdd:cd05939   405 VEVPG-VEGRAGMAAIV-DPERKV-DLDRFSAVLA----KSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQK 469
AACS cd05943
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ...
55-609 2.23e-12

Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.


Pssm-ID: 341265 [Multi-domain]  Cd Length: 629  Bit Score: 69.99  E-value: 2.23e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  55 PEYF-----NFAKDVLdqwtdkekAGKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPR 129
Cdd:cd05943    62 ARWFpgarlNYAENLL--------RHADADDPAAIYAAEDGERTEVTWAELRRRVARLAAAL-RALGVKPGDRVAGYLPN 132
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 130 VPEwwlANVACLRT---GTVLIPGTTQLTQKDILYRLQSSKANCIITNDV---------LAPAVDAVASKCENLHSKLIV 197
Cdd:cd05943   133 IPE---AVVAMLATasiGAIWSSCSPDFGVPGVLDRFGQIEPKVLFAVDAytyngkrhdVREKVAELVKGLPSLLAVVVV 209
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 198 SENSREGWGNLKELMKvflchpgWSTVARSWLTATSTSLvqailsllsswDYRHASDSHTcvktkhneiMAIFFTSGTSG 277
Cdd:cd05943   210 PYTVAAGQPDLSKIAK-------ALTLEDFLATGAAGEL-----------EFEPLPFDHP---------LYILYSSGTTG 262
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 278 YPKmtAHTHSSFGLGLSVNGRFWL--DLTPSDVM-WNTSdTGWAksAWSSVFSPWIQGACVFTHHLPRFEPTS--ILQTL 352
Cdd:cd05943   263 LPK--CIVHGAGGTLLQHLKEHILhcDLRPGDRLfYYTT-CGWM--MWNWLVSGLAVGATIVLYDGSPFYPDTnaLWDLA 337
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 353 SKYPITVFCSAPTVYRMLVQNDI---TSYKFKSLKHCVSAGEPITPD----VTEKWrnKTGLDIYEGYGQTEtvlICGNF 425
Cdd:cd05943   338 DEEGITVFGTSAKYLDALEKAGLkpaETHDLSSLRTILSTGSPLKPEsfdyVYDHI--KPDVLLASISGGTD---IISCF 412
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 426 KGMK----IKPGSMGKPSPAFDVKIVDVNGNVLPpGQEGDIGI-QVLPNRPfglfTHYVDNPSktASTLRGNFYIT---- 496
Cdd:cd05943   413 VGGNpllpVYRGEIQCRGLGMAVEAFDEEGKPVW-GEKGELVCtKPFPSMP----VGFWNDPD--GSRYRAAYFAKypgv 485
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 497 ---GDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDykshdqE 573
Cdd:cd05943   486 wahGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDERVILFVKLREG------V 559
                         570       580       590       600
                  ....*....|....*....|....*....|....*....|
gi 1370468924 574 QLIKEIQEHVKKTTAPYKYPRKV--EFIQ--ELPKTISGK 609
Cdd:cd05943   560 ELDDELRKRIRSTIRSALSPRHVpaKIIAvpDIPRTLSGK 599
PLN03052 PLN03052
acetate--CoA ligase; Provisional
72-618 3.81e-11

acetate--CoA ligase; Provisional


Pssm-ID: 215553 [Multi-domain]  Cd Length: 728  Bit Score: 66.26  E-value: 3.81e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  72 EKAGKKPSNPAFWWINRNGEEM---RWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEWWLANVACLRTGTVLI 148
Cdd:PLN03052  183 PKPSKTDDSIAIIWRDEGSDDLpvnRMTLSELRSQVSRVANAL-DALGFEKGDAIAIDMPMNVHAVIIYLAIILAGCVVV 261
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 149 PGTTQLTQKDILYRLQSSKANCIITNDVL-----------------APAVDAVASKCENLHSKLivsensREGwgnlkel 211
Cdd:PLN03052  262 SIADSFAPSEIATRLKISKAKAIFTQDVIvrggksiplysrvveakAPKAIVLPADGKSVRVKL------REG------- 328
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 212 mkvflchpgwstvARSWLtatstslvqailSLLSSWDYRHASDSHTCVKTKHNEIMAIFFTSGTSGYPKMTAHTHSSfGL 291
Cdd:PLN03052  329 -------------DMSWD------------DFLARANGLRRPDEYKAVEQPVEAFTNILFSSGTTGEPKAIPWTQLT-PL 382
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 292 GLSVNGRFWLDLTPSDVM-WNTsDTGWAKSAWSsVFSPWIQGACvfthhLPRFEPTSILQTLSKY----PITVFCSAPTV 366
Cdd:PLN03052  383 RAAADAWAHLDIRKGDIVcWPT-NLGWMMGPWL-VYASLLNGAT-----LALYNGSPLGRGFAKFvqdaKVTMLGTVPSI 455
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 367 YRMLVQNDITS-YKFKSLKHCVSAGEPITPD----VTEKWRNKTgldIYEGYGQTEtvlICGNF-KGMKIKPGSMGK--- 437
Cdd:PLN03052  456 VKTWKNTNCMAgLDWSSIRCFGSTGEASSVDdylwLMSRAGYKP---IIEYCGGTE---LGGGFvTGSLLQPQAFAAfst 529
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 438 PSPAFDVKIVDVNGNVLPPGQEGdIGIQVLPNRPFGLFT----------HYVDNPSKTASTLRGNfyitGDRGYMDKDGY 507
Cdd:PLN03052  530 PAMGCKLFILDDSGNPYPDDAPC-TGELALFPLMFGASStllnadhykvYFKGMPVFNGKILRRH----GDIFERTSGGY 604
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 508 FWFVARADDVILSSGYRIGPFEVENALNE-HPSVAESAVVSSPDPIRG--EVVKAFVVLNPDYKSHDQEQLIKEIQEHVK 584
Cdd:PLN03052  605 YRAHGRADDTMNLGGIKVSSVEIERVCNAaDESVLETAAIGVPPPGGGpeQLVIAAVLKDPPGSNPDLNELKKIFNSAIQ 684
                         570       580       590
                  ....*....|....*....|....*....|....
gi 1370468924 585 KTTAPYKYPRKVEFIQELPKTISGKTKRNELRKK 618
Cdd:PLN03052  685 KKLNPLFKVSAVVIVPSFPRTASNKVMRRVLRQQ 718
hsFATP2a_ACSVL_like cd05938
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ...
91-594 8.39e-11

Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.


Pssm-ID: 341261 [Multi-domain]  Cd Length: 537  Bit Score: 64.62  E-value: 8.39e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  91 EEMRWSFEELGSLSRKFANILSEACSLQRGDRVILILPRVPEW---WLAnVACLRTGTVLIPgtTQLTQKDILYRLQSSK 167
Cdd:cd05938     2 EGETYTYRDVDRRSNQAARALLAHAGLRPGDTVALLLGNEPAFlwiWLG-LAKLGCPVAFLN--TNIRSKSLLHCFRCCG 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 168 ANCIITNDVLAPAVDAV--ASKCENLHSKLIVSENSREGWGNLKELMKvflchpgwstvarswltATSTSLVQAILSLLS 245
Cdd:cd05938    79 AKVLVVAPELQEAVEEVlpALRADGVSVWYLSHTSNTEGVISLLDKVD-----------------AASDEPVPASLRAHV 141
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 246 SWdyrhasdSHTCVktkhneimaIFFTSGTSGYPKMTAHTHSSFglgLSVNGRFWL-DLTPSDVMWNTSDTgWAKSAWSS 324
Cdd:cd05938   142 TI-------KSPAL---------YIYTSGTTGLPKAARISHLRV---LQCSGFLSLcGVTADDVIYITLPL-YHSSGFLL 201
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 325 VFSPWIQ-GA-CVFThhlPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQndiTSYKFKSLKHCV--SAGEPITPDVTEK 400
Cdd:cd05938   202 GIGGCIElGAtCVLK---PKFSASQFWDDCRKHNVTVIQYIGELLRYLCN---QPQSPNDRDHKVrlAIGNGLRADVWRE 275
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 401 WRNKTG-LDIYEGYGQTETVLICGNFKGmkiKPGSMGKPS-------P----AFDVK----IVDVNGNVLP--PGQEGDI 462
Cdd:cd05938   276 FLRRFGpIRIREFYGSTEGNIGFFNYTG---KIGAVGRVSylykllfPfeliKFDVEkeepVRDAQGFCIPvaKGEPGLL 352
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 463 GIQVLPNRPFglfTHYVDNPSKTASTL------RGNFYI-TGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALN 535
Cdd:cd05938   353 VAKITQQSPF---LGYAGDKEQTEKKLlrdvfkKGDVYFnTGDLLVQDQQNFLYFHDRVGDTFRWKGENVATTEVADVLG 429
                         490       500       510       520       530       540
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1370468924 536 EHPSVAESAV--VSSPDpIRGEVVKAFVVLNPDYkSHDQEQLIkeiqEHVKKTTAPYKYPR 594
Cdd:cd05938   430 LLDFLQEVNVygVTVPG-HEGRIGMAAVKLKPGH-EFDGKKLY----QHVREYLPAYARPR 484
PRK07768 PRK07768
long-chain-fatty-acid--CoA ligase; Validated
345-612 1.09e-10

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236091 [Multi-domain]  Cd Length: 545  Bit Score: 64.25  E-value: 1.09e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 345 PTSILQTLSKYPITV-----FCSAPTVYRMLVQNDITSYKFKSLKHCVSAGEPITPDVTEKWRN---KTGLD---IYEGY 413
Cdd:PRK07768  235 PLLWAELISKYRGTMtaapnFAYALLARRLRRQAKPGAFDLSSLRFALNGAEPIDPADVEDLLDagaRFGLRpeaILPAY 314
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 414 GQTETVLI-----CGNfkGMKI------------------KPG-----SMGKPSPAFDVKIVDVNGNVLPPGQEGDI--- 462
Cdd:PRK07768  315 GMAEATLAvsfspCGA--GLVVdevdadllaalrravpatKGNtrrlaTLGPPLPGLEVRVVDEDGQVLPPRGVGVIelr 392
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 463 GIQVLPnrpfglftHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAE 542
Cdd:PRK07768  393 GESVTP--------GYLTMDGFIPAQDADGWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIYPTDIERAAARVEGVRP 464
                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1370468924 543 SAVVSSPDPiRGEVVKAFVVLNPDYKSHDQE---QLIKEIQEHVKKTTApyKYPRKVEFIQ--ELPKTISGKTKR 612
Cdd:PRK07768  465 GNAVAVRLD-AGHSREGFAVAVESNAFEDPAevrRIRHQVAHEVVAEVG--VRPRNVVVLGpgSIPKTPSGKLRR 536
PRK09192 PRK09192
fatty acyl-AMP ligase;
436-617 1.68e-10

fatty acyl-AMP ligase;


Pssm-ID: 236403 [Multi-domain]  Cd Length: 579  Bit Score: 63.87  E-value: 1.68e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 436 GKPSPAFDVKIVDVNGNVLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGNFYITGDRGYMdKDGYFWFVARAD 515
Cdd:PRK09192  388 GKALPGHEIEIRNEAGMPLPERVVGHICV-----RGPSLMSGYFRDEESQDVLAADGWLDTGDLGYL-LDGYLYITGRAK 461
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 516 DVILSSGYRIGPFEVENALNEHPSV--AESAVVSSPDPiRGEVVKAFV---VLNPDykshDQEQLIKEIQEHVKKTTApy 590
Cdd:PRK09192  462 DLIIINGRNIWPQDIEWIAEQEPELrsGDAAAFSIAQE-NGEKIVLLVqcrISDEE----RRGQLIHALAALVRSEFG-- 534
                         170       180
                  ....*....|....*....|....*....
gi 1370468924 591 kYPRKVEFI--QELPKTISGKTKRNELRK 617
Cdd:PRK09192  535 -VEAAVELVppHSLPRTSSGKLSRAKAKK 562
FATP_chFAT1_like cd05937
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ...
384-620 4.74e-09

Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.


Pssm-ID: 341260 [Multi-domain]  Cd Length: 468  Bit Score: 58.98  E-value: 4.74e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 384 KHCVSA--GEPITPDVTEKWRNKTGL-DIYEGYGQTETVLICGNFKGMKIKPGSMGKPSP------AFDVKIVDVNGN-- 452
Cdd:cd05937   201 DHKVRVawGNGLRPDIWERFRERFNVpEIGEFYAATEGVFALTNHNVGDFGAGAIGHHGLirrwkfENQVVLVKMDPEtd 280
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 453 ------------VLPPGQEGDIgIQVLPNRPFGLFTHYVDNPSKTASTL------RGN-FYITGDRGYMDKDGYFWFVAR 513
Cdd:cd05937   281 dpirdpktgfcvRAPVGEPGEM-LGRVPFKNREAFQGYLHNEDATESKLvrdvfrKGDiYFRTGDLLRQDADGRWYFLDR 359
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 514 ADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDP-IRGEVVKAFVVLNPdyKSHDQEQLIK-EIQEHVKKTTAPYK 591
Cdd:cd05937   360 LGDTFRWKSENVSTTEVADVLGAHPDIAEANVYGVKVPgHDGRAGCAAITLEE--SSAVPTEFTKsLLASLARKNLPSYA 437
                         250       260
                  ....*....|....*....|....*....
gi 1370468924 592 YPRKVEFIQELPKTISGKTKRNELRKKEW 620
Cdd:cd05937   438 VPLFLRLTEEVATTDNHKQQKGVLRDEGV 466
FCS cd05921
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ...
271-566 5.70e-09

Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.


Pssm-ID: 341245 [Multi-domain]  Cd Length: 561  Bit Score: 58.98  E-value: 5.70e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 271 FTSGTSGYPKMTAHTHSSFGLGLSVNGRFWLDLTPSD-VMWNtsdtgWAksAWSSVFSpwiqGACVF--------THHLP 341
Cdd:cd05921   172 FTSGSTGLPKAVINTQRMLCANQAMLEQTYPFFGEEPpVLVD-----WL--PWNHTFG----GNHNFnlvlynggTLYID 240
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 342 RFEPT-----SILQTLSKYPITVFCSAPTVYRMLVQ---NDITSYK--FKSLKHCVSAGEPITPDVTEKWRN----KTGL 407
Cdd:cd05921   241 DGKPMpggfeETLRNLREISPTVYFNVPAGWEMLVAaleKDEALRRrfFKRLKLMFYAGAGLSQDVWDRLQAlavaTVGE 320
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 408 DI--YEGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVlppgqEGDI-GIQVLPNrpfglfthYVDNPSK 484
Cdd:cd05921   321 RIpmMAGLGATETAPTATFTHWPTERSGLIGLPAPGTELKLVPSGGKY-----EVRVkGPNVTPG--------YWRQPEL 387
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 485 TASTL-RGNFYITGDRGYM----DKDGYFWFVAR-ADDVILSSG--YRIGPFEVEnALNEHPSVAESAVVSSPDpirGEV 556
Cdd:cd05921   388 TAQAFdEEGFYCLGDAAKLadpdDPAKGLVFDGRvAEDFKLASGtwVSVGPLRAR-AVAACAPLVHDAVVAGED---RAE 463
                         330
                  ....*....|
gi 1370468924 557 VKAFVVLNPD 566
Cdd:cd05921   464 VGALVFPDLL 473
PRK06334 PRK06334
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
259-537 3.05e-08

long chain fatty acid--[acyl-carrier-protein] ligase; Validated


Pssm-ID: 180533 [Multi-domain]  Cd Length: 539  Bit Score: 56.75  E-value: 3.05e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 259 VKTKHNEIMA-IFFTSGTSGYPKMTAHTHSSfglgLSVNGRFWLDL---TPSDVMWNTSDTGWAKSAWSSVFSPWIQGAC 334
Cdd:PRK06334  177 VSDKDPEDVAvILFTSGTEKLPKGVPLTHAN----LLANQRACLKFfspKEDDVMMSFLPPFHAYGFNSCTLFPLLSGVP 252
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 335 VFTHHLPrFEPTSILQTLSKYPITVFCSAPTVYRMLVQndiTSYK----FKSLKHCVSAGEPITPDVTEK-WRNKTGLDI 409
Cdd:PRK06334  253 VVFAYNP-LYPKKIVEMIDEAKVTFLGSTPVFFDYILK---TAKKqescLPSLRFVVIGGDAFKDSLYQEaLKTFPHIQL 328
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 410 YEGYGQTE-TVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVlpPGQEGDIGIQVLpnRPFGLFTHYVDN-PSKTAS 487
Cdd:PRK06334  329 RQGYGTTEcSPVITINTVNSPKHESCVGMPIRGMDVLIVSEETKV--PVSSGETGLVLT--RGTSLFSGYLGEdFGQGFV 404
                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1370468924 488 TLRG-NFYITGDRGYMDKDGYFWFVARaddviLSSGYRIGPFEV-----ENALNEH 537
Cdd:PRK06334  405 ELGGeTWYVTGDLGYVDRHGELFLKGR-----LSRFVKIGAEMVslealESILMEG 455
PRK03584 PRK03584
acetoacetate--CoA ligase;
59-609 5.63e-08

acetoacetate--CoA ligase;


Pssm-ID: 235134 [Multi-domain]  Cd Length: 655  Bit Score: 55.96  E-value: 5.63e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  59 NFAKDVLDQwtdkekagKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANILsEACSLQRGDRVILILPRVPEwwlANV 138
Cdd:PRK03584   87 NYAENLLRH--------RRDDRPAIIFRGEDGPRRELSWAELRRQVAALAAAL-RALGVGPGDRVAAYLPNIPE---TVV 154
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 139 ACLRT---GTVLipgttqltqkdilyrlqSSkanCiitndvlAP--AVDAVASKcenlhsklivsensregWGNLKElmK 213
Cdd:PRK03584  155 AMLATaslGAIW-----------------SS---C-------SPdfGVQGVLDR-----------------FGQIEP--K 188
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 214 VFLCHPGWS----------TVARswLTATSTSLVQAILsllssWDYRHASDSHTCVKTKH--NEIMA------------- 268
Cdd:PRK03584  189 VLIAVDGYRyggkafdrraKVAE--LRAALPSLEHVVV-----VPYLGPAAAAAALPGALlwEDFLApaeaaelefepvp 261
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 269 ------IFFTSGTSGYPKMTAHTHssfG---------LGLSvngrfwLDLTPSD-VMWNTSdTGWAksAWSSVFSPWIQG 332
Cdd:PRK03584  262 fdhplwILYSSGTTGLPKCIVHGH---GgillehlkeLGLH------CDLGPGDrFFWYTT-CGWM--MWNWLVSGLLVG 329
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 333 ACVFTHHLPRFEPTsiLQTL----SKYPITVFCSAPTVYRMLVQNDIT---SYKFKSLKHCVSAGEPITPDVTEkWrnkt 405
Cdd:PRK03584  330 ATLVLYDGSPFYPD--PNVLwdlaAEEGVTVFGTSAKYLDACEKAGLVpgeTHDLSALRTIGSTGSPLPPEGFD-W---- 402
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 406 gldIYEGYGQ----------TEtvlICGNFKG----MKIKPGSMGKPSPAFDVKIVDVNGN-VLppGQEGDIGI-QVLPN 469
Cdd:PRK03584  403 ---VYEHVKAdvwlasisggTD---ICSCFVGgnplLPVYRGEIQCRGLGMAVEAWDEDGRpVV--GEVGELVCtKPFPS 474
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 470 RPFGlFTHYVDNpsktaSTLRGNFYIT-------GDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAE 542
Cdd:PRK03584  475 MPLG-FWNDPDG-----SRYRDAYFDTfpgvwrhGDWIEITEHGGVVIYGRSDATLNRGGVRIGTAEIYRQVEALPEVLD 548
                         570       580       590       600       610       620
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1370468924 543 SAVVSSPDPIRGEVVKAFVVLNPDYKSHDQeqLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGK 609
Cdd:PRK03584  549 SLVIGQEWPDGDVRMPLFVVLAEGVTLDDA--LRARIRTTIRTNLSPRHVPDKIIAVPDIPRTLSGK 613
PRK05691 PRK05691
peptide synthase; Validated
73-616 5.73e-08

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 56.33  E-value: 5.73e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924   73 KAGKKPSNPAFWWINRNGEEMR-WSFEELGSLSRKFANILSEACSLqrGDRVILILPRVPEWWLANVACLRTGTVLIPG- 150
Cdd:PRK05691    18 RAAQTPDRLALRFLADDPGEGVvLSYRDLDLRARTIAAALQARASF--GDRAVLLFPSGPDYVAAFFGCLYAGVIAVPAy 95
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  151 ----TTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVaskcENLHSklivsensregwGNLKELMKVFLCHPGwstVAR 226
Cdd:PRK05691    96 ppesARRHHQERLLSIIADAEPRLLLTVADLRDSLLQM----EELAA------------ANAPELLCVDTLDPA---LAE 156
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  227 SWLTATstslVQAilsllsswdyrhasdshtcvktkhNEIMAIFFTSGTSGYPKMTAHTHSSfglgLSVNGR-----FWL 301
Cdd:PRK05691   157 AWQEPA----LQP------------------------DDIAFLQYTSGSTALPKGVQVSHGN----LVANEQlirhgFGI 204
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  302 DLTPSDVM--WNT--SDTGWAKSAWSSVFS--PwiqgaCVF---THHLPRfePTSILQTLSKYPITV---------FCSA 363
Cdd:PRK05691   205 DLNPDDVIvsWLPlyHDMGLIGGLLQPIFSgvP-----CVLmspAYFLER--PLRWLEAISEYGGTIsggpdfayrLCSE 277
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  364 PTVYRMLVQNDITSYKFkslkhCVSAGEPITPDVTEKWRNK---TGLD---IYEGYGQTE-TVLICGNFKGMKI------ 430
Cdd:PRK05691   278 RVSESALERLDLSRWRV-----AYSGSEPIRQDSLERFAEKfaaCGFDpdsFFASYGLAEaTLFVSGGRRGQGIpaleld 352
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  431 ---------KPG------SMGKPSPAFDVKIVD-VNGNVLPPGQEGDI---GiqvlPNRPFGlfthYVDNPSKTAST--- 488
Cdd:PRK05691   353 aealarnraEPGtgsvlmSCGRSQPGHAVLIVDpQSLEVLGDNRVGEIwasG----PSIAHG----YWRNPEASAKTfve 424
                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  489 LRGNFYI-TGDRGYMdKDGYFWFVARADDVILSSGYRIGPFEVENALNEhpsvaESAVVsspdpiRGEVVKAFVVlnpdy 567
Cdd:PRK05691   425 HDGRTWLrTGDLGFL-RDGELFVTGRLKDMLIVRGHNLYPQDIEKTVER-----EVEVV------RKGRVAAFAV----- 487
                          570       580       590       600       610       620
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1370468924  568 kSHDQEQLI---KEIQEHVKKTTAP---YKYPRKV--EFIQE------------LPKTISGKTKRNELR 616
Cdd:PRK05691   488 -NHQGEEGIgiaAEISRSVQKILPPqalIKSIRQAvaEACQEapsvvlllnpgaLPKTSSGKLQRSACR 555
A_NRPS_alphaAR cd17647
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ...
271-615 7.34e-08

Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.


Pssm-ID: 341302 [Multi-domain]  Cd Length: 520  Bit Score: 55.22  E-value: 7.34e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 271 FTSGTSGYPKMTAHTHSSFGLGLS-VNGRFwlDLTPSDVMwnTSDTGWAKSAWS-SVFSPWIQGACVFthhLPRFE---- 344
Cdd:cd17647   116 FTSGSEGIPKGVLGRHFSLAYYFPwMAKRF--NLSENDKF--TMLSGIAHDPIQrDMFTPLFLGAQLL---VPTQDdigt 188
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 345 PTSILQTLSKYPITVFCSAPTVYRMLVQNDITSykFKSLKHCVSAGEPITP-DVTEKWRNKTGLDIYEGYGQTETVLICG 423
Cdd:cd17647   189 PGRLAEWMAKYGATVTHLTPAMGQLLTAQATTP--FPKLHHAFFVGDILTKrDCLRLQTLAENVRIVNMYGTTETQRAVS 266
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 424 NF--KGMKIKPG---SMGKPSPA----FDVKIVDVNGN----VLPPGQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLR 490
Cdd:cd17647   267 YFevPSRSSDPTflkNLKDVMPAgrgmLNVQLLVVNRNdrtqICGIGEVGEIYV-----RAGGLAEGYRGLPELNKEKFV 341
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 491 GNFYITGDR-GYMDKDG-----YFWF-----------------------VARADDVILSSGYRIGPFEVENALNEHPSVA 541
Cdd:cd17647   342 NNWFVEPDHwNYLDKDNnepwrQFWLgprdrlyrtgdlgrylpngdcecCGRADDQVKIRGFRIELGEIDTHISQHPLVR 421
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 542 ES------------AVVS--SPDPIRGEVVKAFVVLNPDYKSHDQ--------EQLIKEIQEHVKKTTAPYKYPRKVEFI 599
Cdd:cd17647   422 ENitlvrrdkdeepTLVSyiVPRFDKPDDESFAQEDVPKEVSTDPivkgligyRKLIKDIREFLKKRLASYAIPSLIVVL 501
                         410
                  ....*....|....*.
gi 1370468924 600 QELPKTISGKTKRNEL 615
Cdd:cd17647   502 DKLPLNPNGKVDKPKL 517
LC_FACS_euk1 cd17639
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ...
383-619 2.27e-07

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.


Pssm-ID: 341294 [Multi-domain]  Cd Length: 507  Bit Score: 53.76  E-value: 2.27e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 383 LKHCVSAGEPITPDvTEKWRNKTGLDIYEGYGQTETvliCGNfkGMKIKPGSM-----GKPSPAFDVKIVDV----NGNV 453
Cdd:cd17639   252 LRYMLSGGAPLSAD-TQEFLNIVLCPVIQGYGLTET---CAG--GTVQDPGDLetgrvGPPLPCCEIKLVDWeeggYSTD 325
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 454 LPPGQeGDIGIQvlpnRPFgLFTHYVDNPSKTASTLRGN--FYiTGDRGYMDKDGYFWFVARADD-VILSSGYRIGPFEV 530
Cdd:cd17639   326 KPPPR-GEILIR----GPN-VFKGYYKNPEKTKEAFDGDgwFH-TGDIGEFHPDGTLKIIDRKKDlVKLQNGEYIALEKL 398
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 531 ENALNEHPSVAESAVVSspDPIRGEVVkAFVVLNpdykshdQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKT 610
Cdd:cd17639   399 ESIYRSNPLVNNICVYA--DPDKSYPV-AIVVPN-------EKHLTKLAEKHGVINSEWEELCEDKKLQKAVLKSLAETA 468

                  ....*....
gi 1370468924 611 KRNELRKKE 619
Cdd:cd17639   469 RAAGLEKFE 477
ACSBG_like cd05933
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ...
379-536 5.34e-07

Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341256 [Multi-domain]  Cd Length: 596  Bit Score: 52.74  E-value: 5.34e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 379 KFKSLKHCV---SAGEPITPDVTEKWrnkTGLDI--YEGYGQTE-----TVLICGNFKgmkikPGSMGKPSPAFDVKIVD 448
Cdd:cd05933   315 KALGLDRCQkffTGAAPISRETLEFF---LSLNIpiMELYGMSEtsgphTISNPQAYR-----LLSCGKALPGCKTKIHN 386
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 449 VNGNvlppGQeGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGNFYI-TGDRGYMDKDGYFWFVARADD-VILSSGYRIG 526
Cdd:cd05933   387 PDAD----GI-GEICF-----WGRHVFMGYLNMEDKTEEAIDEDGWLhSGDLGKLDEDGFLYITGRIKElIITAGGENVP 456
                         170
                  ....*....|
gi 1370468924 527 PFEVENALNE 536
Cdd:cd05933   457 PVPIEDAVKK 466
PLN02430 PLN02430
long-chain-fatty-acid-CoA ligase
387-617 1.79e-06

long-chain-fatty-acid-CoA ligase


Pssm-ID: 178049 [Multi-domain]  Cd Length: 660  Bit Score: 50.97  E-value: 1.79e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 387 VSAGEPITPDVTEKWRNKTGLDIYEGYGQTETvliCGnfkgmkikPGSMGKPSPAFDVKIVDVNG--NVLPPGQEGDIGI 464
Cdd:PLN02430  389 ISGGAPLSTEIEEFLRVTSCAFVVQGYGLTET---LG--------PTTLGFPDEMCMLGTVGAPAvyNELRLEEVPEMGY 457
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 465 QVLPNRPFG--------LFTHYVDNPSKTASTLRGNFYITGDRGYMDKDGYFWFVARADDVI-LSSGYRIGPFEVENALN 535
Cdd:PLN02430  458 DPLGEPPRGeicvrgkcLFSGYYKNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIkLSQGEYVALEYLENVYG 537
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 536 EHPSVAESAV-----------VSSPDPirgEVVKAFVVLNPDYKSHDQEQLIKEIQEHV---KKTTAPYKYPRKVEFIQ- 600
Cdd:PLN02430  538 QNPIVEDIWVygdsfksmlvaVVVPNE---ENTNKWAKDNGFTGSFEELCSLPELKEHIlseLKSTAEKNKLRGFEYIKg 614
                         250       260
                  ....*....|....*....|....*...
gi 1370468924 601 --------ELPK---TISGKTKRNELRK 617
Cdd:PLN02430  615 viletkpfDVERdlvTATLKKRRNNLLK 642
PLN02736 PLN02736
long-chain acyl-CoA synthetase
387-559 7.96e-06

long-chain acyl-CoA synthetase


Pssm-ID: 178337 [Multi-domain]  Cd Length: 651  Bit Score: 48.94  E-value: 7.96e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 387 VSAGEPITPDVTEKWRNKTGLDIYEGYGQTET-VLICGNFKGMKIKpGSMGKPSPAFDVKIVDVngnvlP---------P 456
Cdd:PLN02736  382 SSGASPLSPDVMEFLRICFGGRVLEGYGMTETsCVISGMDEGDNLS-GHVGSPNPACEVKLVDV-----PemnytsedqP 455
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 457 GQEGDIGIqvlpnRPFGLFTHYVDNPSKTASTLRGNFYI-TGDRGYMDKDGYFWFVARADDVI-LSSGYRIGPFEVENAL 534
Cdd:PLN02736  456 YPRGEICV-----RGPIIFKGYYKDEVQTREVIDEDGWLhTGDIGLWLPGGRLKIIDRKKNIFkLAQGEYIAPEKIENVY 530
                         170       180       190
                  ....*....|....*....|....*....|....*.
gi 1370468924 535 NEHPSVAESAV-----------VSSPDPirgEVVKA 559
Cdd:PLN02736  531 AKCKFVAQCFVygdslnsslvaVVVVDP---EVLKA 563
PRK05851 PRK05851
long-chain-fatty acid--ACP ligase MbtM;
382-617 2.39e-05

long-chain-fatty acid--ACP ligase MbtM;


Pssm-ID: 180289 [Multi-domain]  Cd Length: 525  Bit Score: 47.45  E-value: 2.39e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 382 SLKHCVSAGEPITPDVTEKWRNKT---GLD---IYEGYGQTE-----TVLICGNfkGMKIKPGSM------------GKP 438
Cdd:PRK05851  273 ALRVALNGGEPVDCDGFERFATAMapfGFDagaAAPSYGLAEstcavTVPVPGI--GLRVDEVTTddgsgarrhavlGNP 350
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 439 SPAFDVKIVDVNGNVLPPGQE-GDIGIqvlpnRPFGLFTHYVDNPSKTastlRGNFYITGDRGYMdKDGYFWFVARADDV 517
Cdd:PRK05851  351 IPGMEVRISPGDGAAGVAGREiGEIEI-----RGASMMSGYLGQAPID----PDDWFPTGDLGYL-VDGGLVVCGRAKEL 420
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 518 ILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGevVKAFVVLNPDYKSHDQEQLIKEIQEHVKKTTApyKYPRKVE 597
Cdd:PRK05851  421 ITVAGRNIFPTEIERVAAQVRGVREGAVVAVGTGEGS--ARPGLVIAAEFRGPDEAGARSEVVQRVASECG--VVPSDVV 496
                         250       260
                  ....*....|....*....|..
gi 1370468924 598 FIQ--ELPKTISGKTKRNELRK 617
Cdd:PRK05851  497 FVApgSLPRTSSGKLRRLAVKR 518
alpha_am_amid TIGR03443
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ...
490-609 8.62e-05

L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.


Pssm-ID: 274582 [Multi-domain]  Cd Length: 1389  Bit Score: 45.83  E-value: 8.62e-05
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924  490 RGNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAE----------------SAVVSSPDPir 553
Cdd:TIGR03443  676 RDRLYRTGDLGRYLPDGNVECCGRADDQVKIRGFRIELGEIDTHLSQHPLVREnvtlvrrdkdeeptlvSYIVPQDKS-- 753
                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1370468924  554 gEVVKAFVVLNPDYKSHDQ--------EQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGK 609
Cdd:TIGR03443  754 -DELEEFKSEVDDEESSDPvvkglikyRKLIKDIREYLKKKLPSYAIPTVIVPLKKLPLNPNGK 816
PLN02387 PLN02387
long-chain-fatty-acid-CoA ligase family protein
388-618 3.29e-04

long-chain-fatty-acid-CoA ligase family protein


Pssm-ID: 215217 [Multi-domain]  Cd Length: 696  Bit Score: 43.95  E-value: 3.29e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 388 SAGEPITPDvTEKWRNKT-GLDIYEGYGQTETvliCG-----NFKGMKIkpGSMGKPSPAFDVKIVD-VNGNVL---PPG 457
Cdd:PLN02387  427 SGGAPLSGD-TQRFINIClGAPIGQGYGLTET---CAgatfsEWDDTSV--GRVGPPLPCCYVKLVSwEEGGYLisdKPM 500
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 458 QEGDIGIQVlPNRPFGlfthYVDNPSKTASTL----RGN--FYiTGDRGYMDKDGYFWFVARADDVI-LSSGYRIGPFEV 530
Cdd:PLN02387  501 PRGEIVIGG-PSVTLG----YFKNQEKTDEVYkvdeRGMrwFY-TGDIGQFHPDGCLEIIDRKKDIVkLQHGEYVSLGKV 574
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370468924 531 ENALNEHPSVaESAVVSSpDPIRGEVVKAFVVLNP-----------DYKSHD----QEQLIKEIQEHVKKTTAPYKY--- 592
Cdd:PLN02387  575 EAALSVSPYV-DNIMVHA-DPFHSYCVALVVPSQQalekwakkagiDYSNFAelceKEEAVKEVQQSLSKAAKAARLekf 652
                         250       260       270
                  ....*....|....*....|....*....|....
gi 1370468924 593 --PRKVEFIQELPKTISG------KTKRNELRKK 618
Cdd:PLN02387  653 eiPAKIKLLPEPWTPESGlvtaalKLKREQIRKK 686
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH