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Conserved domains on  [gi|1279794264|ref|XP_022950286|]
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iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita moschata]

Protein Classification

iron-sulfur cluster assembly accessory protein( domain architecture ID 10015430)

iron-sulfur cluster assembly accessory protein from the HesB/IscA family is involved in the assembly of iron-sulfur proteins

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 66-170 2.82e-53

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


:

Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 164.68  E-value: 2.82e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  66 LTDNALKHLNKMRSERNED-LCLRIGVRQGGCSGMSYTMEFENRANarPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSD 144
Cdd:TIGR00049   2 LTDSAAKRIKALLAGEGEPnLGLRVGVKGGGCSGLQYGLEFDDEPN--EDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVE 79

                          90       100
                  ....*....|....*....|....*.
gi 1279794264 145 ALIGGGFSFKNPNATQTCGCGKSFAA 170
Cdd:TIGR00049  80 ELLGSGFTFTNPNAKGTCGCGKSFSV 105
 
Name Accession Description Interval E-value
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 66-170 2.82e-53

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 164.68  E-value: 2.82e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  66 LTDNALKHLNKMRSERNED-LCLRIGVRQGGCSGMSYTMEFENRANarPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSD 144
Cdd:TIGR00049   2 LTDSAAKRIKALLAGEGEPnLGLRVGVKGGGCSGLQYGLEFDDEPN--EDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVE 79

                          90       100
                  ....*....|....*....|....*.
gi 1279794264 145 ALIGGGFSFKNPNATQTCGCGKSFAA 170
Cdd:TIGR00049  80 ELLGSGFTFTNPNAKGTCGCGKSFSV 105
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 63-170 1.55e-52

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 162.63  E-value: 1.55e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  63 AISLTDNALKHLNK-MRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANarPDDSIIEYNGFVIVCDPKSLLFLFGMQLD 141
Cdd:COG0316     1 PITLTDAAAKRIKRlLAKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPN--EDDLVFEQDGVKVVVDPKSLPYLDGTEID 78

                          90       100
                  ....*....|....*....|....*....
gi 1279794264 142 YSDALIGGGFSFKNPNATQTCGCGKSFAA 170
Cdd:COG0316    79 YVEELLGSGFKFNNPNAKSSCGCGESFSV 107
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 55-170 1.24e-30

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 107.32  E-value: 1.24e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  55 PASEGIAPAISLTDNALKHLNKMRSER-NEDLCLRIGVRQGGCSGMSYTMEFENRANArpDDSIIEYNGFVIVCDPKSLL 133
Cdd:PRK13623    1 MMSDDVPLPLVFTDAAAAKVKELIEEEgNPDLKLRVYITGGGCSGFQYGFTFDEQVNE--DDTTIEKQGVTLVVDPMSLQ 78

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1279794264 134 FLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAA 170
Cdd:PRK13623   79 YLVGAEVDYTEGLEGSRFVIKNPNAKTTCGCGSSFSI 115
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 64-166 6.09e-21

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 82.31  E-value: 6.09e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  64 ISLTDNALKHLNKMRSERNEDLCLRI-----GVRQGGCS-GMSYTMEFENRANArPDDSIIEYNGFVIVCDPKSLLFLF- 136
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKELRLDVddgggPYSKGGCSiGGKFSLVLVDEPDP-DYDEVIESNGGPIYVDSYSLPFLDe 81

                          90       100       110
                  ....*....|....*....|....*....|
gi 1279794264 137 GMQLDYSDALIGGGFSFKNPNATQTCGCGK 166
Cdd:pfam01521  82 GLTLDFVEDLGTLGLKSDNGNLDGNVGCGD 111
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 64-165 2.42e-04

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 38.46  E-value: 2.42e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  64 ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFEnraNARPDDSIIEYNGFVIVCDpKSLLFLFG-MQLDY 142
Cdd:NF038090    2 LEVTDLASRKLKEYFADKKIDSPIRVFLNQGGUAGPSLGLALD---EPKENDEVFEQDGLTFVID-KELLEQCGpIKVDF 77

                          90       100
                  ....*....|....*....|...
gi 1279794264 143 SDAliGGGFSFKNPNATQTCGCG 165
Cdd:NF038090   78 IDC--RSGFSITSSMPLGGGGCG 98
 
Name Accession Description Interval E-value
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 66-170 2.82e-53

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 164.68  E-value: 2.82e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  66 LTDNALKHLNKMRSERNED-LCLRIGVRQGGCSGMSYTMEFENRANarPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSD 144
Cdd:TIGR00049   2 LTDSAAKRIKALLAGEGEPnLGLRVGVKGGGCSGLQYGLEFDDEPN--EDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVE 79

                          90       100
                  ....*....|....*....|....*.
gi 1279794264 145 ALIGGGFSFKNPNATQTCGCGKSFAA 170
Cdd:TIGR00049  80 ELLGSGFTFTNPNAKGTCGCGKSFSV 105
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 63-170 1.55e-52

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 162.63  E-value: 1.55e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  63 AISLTDNALKHLNK-MRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANarPDDSIIEYNGFVIVCDPKSLLFLFGMQLD 141
Cdd:COG0316     1 PITLTDAAAKRIKRlLAKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPN--EDDLVFEQDGVKVVVDPKSLPYLDGTEID 78

                          90       100
                  ....*....|....*....|....*....
gi 1279794264 142 YSDALIGGGFSFKNPNATQTCGCGKSFAA 170
Cdd:COG0316    79 YVEELLGSGFKFNNPNAKSSCGCGESFSV 107
IscA TIGR02011
iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC ...
 64-168 5.40e-32

iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC system for iron-sulfur cluster assembly. The ISC system consists of IscRASU, HscAB and an Isc-specific ferredoxin. IscA previously was believed to act as a scaffold and now is seen as an iron donor protein. This clade is limited to the proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 213674  Cd Length: 105  Bit Score: 110.68  E-value: 5.40e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  64 ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANarPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYS 143
Cdd:TIGR02011   1 ITLTDSAAARVNTFLANRGKGFGLRLGVKTSGCSGMAYVLEFVDEPT--PDDIVFEDKGVKIVIDGKSLQYLDGTQLDFV 78

                          90       100
                  ....*....|....*....|....*
gi 1279794264 144 DALIGGGFSFKNPNATQTCGCGKSF 168
Cdd:TIGR02011  79 KEGLNEGFKFTNPNVKDECGCGESF 103
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 55-170 1.24e-30

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 107.32  E-value: 1.24e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  55 PASEGIAPAISLTDNALKHLNKMRSER-NEDLCLRIGVRQGGCSGMSYTMEFENRANArpDDSIIEYNGFVIVCDPKSLL 133
Cdd:PRK13623    1 MMSDDVPLPLVFTDAAAAKVKELIEEEgNPDLKLRVYITGGGCSGFQYGFTFDEQVNE--DDTTIEKQGVTLVVDPMSLQ 78

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1279794264 134 FLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAA 170
Cdd:PRK13623   79 YLVGAEVDYTEGLEGSRFVIKNPNAKTTCGCGSSFSI 115
sufA_proteo TIGR01997
FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of ...
 64-168 5.70e-28

FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of iron-sulfur cluster biosynthesis. This system performs FeS biosynthesis even during oxidative stress and tends to be absent in obligate anaerobic and microaerophilic bacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 131052  Cd Length: 107  Bit Score: 100.29  E-value: 5.70e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  64 ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANArpDDSIIEYNGFVIVCDPKSLLFLFGMQLDYS 143
Cdd:TIGR01997   3 ITLTDAAAIHIRELVAKRPEAVGIRLGVKKTGCAGMEYVLDLVSEPKK--DDDLIEHDGAKVFVAPEAVLFILGTQVDFV 80

                          90       100
                  ....*....|....*....|....*
gi 1279794264 144 DALIGGGFSFKNPNATQTCGCGKSF 168
Cdd:TIGR01997  81 RTTLRQGFKFNNPNATSACGCGESF 105
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
63-168 6.66e-28

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 100.33  E-value: 6.66e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  63 AISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANarPDDSIIEYNGFVIVCDPKSLLFLFGMQLDY 142
Cdd:PRK09502    2 SITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEFVDEPT--PEDIVFEDKGVKVVVDGKSLQFLDGTQLDF 79

                          90       100
                  ....*....|....*....|....*.
gi 1279794264 143 SDALIGGGFSFKNPNATQTCGCGKSF 168
Cdd:PRK09502   80 VKEGLNEGFKFTNPNVKDECGCGESF 105
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 64-166 6.09e-21

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 82.31  E-value: 6.09e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  64 ISLTDNALKHLNKMRSERNEDLCLRI-----GVRQGGCS-GMSYTMEFENRANArPDDSIIEYNGFVIVCDPKSLLFLF- 136
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKELRLDVddgggPYSKGGCSiGGKFSLVLVDEPDP-DYDEVIESNGGPIYVDSYSLPFLDe 81

                          90       100       110
                  ....*....|....*....|....*....|
gi 1279794264 137 GMQLDYSDALIGGGFSFKNPNATQTCGCGK 166
Cdd:pfam01521  82 GLTLDFVEDLGTLGLKSDNGNLDGNVGCGD 111
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 17-168 1.50e-19

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 80.36  E-value: 1.50e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  17 RALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVS-VPAAPASEGIAPAISLTDNALKHLNKMRSER--NEDLCLRIGVRQ 93
Cdd:PLN03082    7 RSLLRRVAPLLAGRIRQNHRLLNFSSSSALKEAStSSSSPSSSASLDAVHMTDNCIRRLKELQTSEpsAEDKMLRLSVET 86

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1279794264  94 GGCSGMSYTMEFENRANarPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFK-NPNATQTCGCGKSF 168
Cdd:PLN03082   87 GGCSGFQYVFELDDKTN--SDDRVFEKDGVKLVVDNISYDFVKGATVDYVEELIRSAFVVStNPSAVGGCSCKSSF 160
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 64-168 1.95e-16

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 71.31  E-value: 1.95e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  64 ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANarPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYS 143
Cdd:PRK09504   18 LTLTPAAAAHIRELMAKQPGMKGVRLGVKQTGCAGFGYVLDSVSEPD--KDDLVFEHDGAKLFVPLQAMPFIDGTEVDYV 95

                          90       100
                  ....*....|....*....|....*
gi 1279794264 144 DALIGGGFSFKNPNATQTCGCGKSF 168
Cdd:PRK09504   96 REGLNQIFKFHNPKAQNECGCGESF 120
PRK11190 PRK11190
iron-sulfur cluster biogenesis protein NfuA;
64-158 6.54e-06

iron-sulfur cluster biogenesis protein NfuA;


Pssm-ID: 183027 [Multi-domain]  Cd Length: 192  Bit Score: 44.24  E-value: 6.54e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  64 ISLTDNALKHLNKMRSERNEDLCLRI-----GVRQGGCsGMSYTmefenRANA-RPDDSIIEYNGFVIVCDPKSLLFLFG 137
Cdd:PRK11190    2 ITISDAAQAHFAKLLANQEEGTQIRVfvinpGTPNAEC-GVSYC-----PPDAvEATDTELKFDGFSAYVDELSAPFLED 75

                          90       100
                  ....*....|....*....|.
gi 1279794264 138 MQLDYSDALIGGGFSFKNPNA 158
Cdd:PRK11190   76 AEIDFVTDQLGSQLTLKAPNA 96
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 64-165 2.42e-04

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 38.46  E-value: 2.42e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1279794264  64 ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFEnraNARPDDSIIEYNGFVIVCDpKSLLFLFG-MQLDY 142
Cdd:NF038090    2 LEVTDLASRKLKEYFADKKIDSPIRVFLNQGGUAGPSLGLALD---EPKENDEVFEQDGLTFVID-KELLEQCGpIKVDF 77

                          90       100
                  ....*....|....*....|...
gi 1279794264 143 SDAliGGGFSFKNPNATQTCGCG 165
Cdd:NF038090   78 IDC--RSGFSITSSMPLGGGGCG 98
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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