forkhead associated (FHA) domain found in microspherule protein 1 (MCRS1) and similar proteins; MCRS1, also called 58 kDa microspherule protein (MSP58), cell cycle-regulated factor p78, or INO80 complex subunit J, is a nuclear RNA-binding protein that interacts with fragile X mental retardation protein in polyribosomal ribonucleoparticles (mRNPs) from neurons. It is a cell-cycle-dependent protein that acts as an inhibitor of TERT telomerase activity. It associates with LPTS/PinX1 and reduces the telomere length. It modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. As part of the NSL complex, MCRS1 may be involved in acetylation of nucleosomal histone H4 on several lysine residues. MCRS1 is also a putative regulatory component of the chromatin remodeling INO80 complex, which is involved in transcriptional regulation, DNA replication, and probably DNA repair. MCRS1 contains an FHA domain at its C-terminus. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1062670006 457 FDSQTLACLCGRLVRYLMRSKEITFGREAKECGVDVDLSLEGPAAKISRRQGTIKMRSNGDFFIANEGKRAIFIDGTPLL 536
Cdd:cd22687     1 FDSGALAVLRGRLVRYLMRSREVTIGRSTKDNQVDIDLSLEGPAWKISRRQAVIKLKNDGDFYIKNEGKRPIYVNGKPVP 80

                          90       100
                  ....*....|....*....|....*
gi 1062670006 537 NGNKTRLAHNCTVEISGLRFTFLVN 561
Cdd:cd22687    81 TGEKAILNNNSLIEIGGLRFVFLIN 105

forkhead associated (FHA) domain found in microspherule protein 1 (MCRS1) and similar proteins; MCRS1, also called 58 kDa microspherule protein (MSP58), cell cycle-regulated factor p78, or INO80 complex subunit J, is a nuclear RNA-binding protein that interacts with fragile X mental retardation protein in polyribosomal ribonucleoparticles (mRNPs) from neurons. It is a cell-cycle-dependent protein that acts as an inhibitor of TERT telomerase activity. It associates with LPTS/PinX1 and reduces the telomere length. It modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. As part of the NSL complex, MCRS1 may be involved in acetylation of nucleosomal histone H4 on several lysine residues. MCRS1 is also a putative regulatory component of the chromatin remodeling INO80 complex, which is involved in transcriptional regulation, DNA replication, and probably DNA repair. MCRS1 contains an FHA domain at its C-terminus. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1062670006 457 FDSQTLACLCGRLVRYLMRSKEITFGREAKECGVDVDLSLEGPAAKISRRQGTIKMRSNGDFFIANEGKRAIFIDGTPLL 536
Cdd:cd22687     1 FDSGALAVLRGRLVRYLMRSREVTIGRSTKDNQVDIDLSLEGPAWKISRRQAVIKLKNDGDFYIKNEGKRPIYVNGKPVP 80

                          90       100
                  ....*....|....*....|....*
gi 1062670006 537 NGNKTRLAHNCTVEISGLRFTFLVN 561
Cdd:cd22687    81 TGEKAILNNNSLIEIGGLRFVFLIN 105

Forkhead associated domain; Found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 1062670006  479 ITFGREAKECgvDVDLSLEGpaakISRRQGTIKMRSNGDFFIANEGKRA-IFIDGTPL 535
Cdd:smart00240   1 VTIGRSSEDC--DIQLDGPS----ISRRHAVIVYDGGGRFYLIDLGSTNgTFVNGKRI 52

forkhead associated (FHA) domain found in microspherule protein 1 (MCRS1) and similar proteins; MCRS1, also called 58 kDa microspherule protein (MSP58), cell cycle-regulated factor p78, or INO80 complex subunit J, is a nuclear RNA-binding protein that interacts with fragile X mental retardation protein in polyribosomal ribonucleoparticles (mRNPs) from neurons. It is a cell-cycle-dependent protein that acts as an inhibitor of TERT telomerase activity. It associates with LPTS/PinX1 and reduces the telomere length. It modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. As part of the NSL complex, MCRS1 may be involved in acetylation of nucleosomal histone H4 on several lysine residues. MCRS1 is also a putative regulatory component of the chromatin remodeling INO80 complex, which is involved in transcriptional regulation, DNA replication, and probably DNA repair. MCRS1 contains an FHA domain at its C-terminus. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1062670006 457 FDSQTLACLCGRLVRYLMRSKEITFGREAKECGVDVDLSLEGPAAKISRRQGTIKMRSNGDFFIANEGKRAIFIDGTPLL 536
Cdd:cd22687     1 FDSGALAVLRGRLVRYLMRSREVTIGRSTKDNQVDIDLSLEGPAWKISRRQAVIKLKNDGDFYIKNEGKRPIYVNGKPVP 80

                          90       100
                  ....*....|....*....|....*
gi 1062670006 537 NGNKTRLAHNCTVEISGLRFTFLVN 561
Cdd:cd22687    81 TGEKAILNNNSLIEIGGLRFVFLIN 105

forkhead associated (FHA) domain superfamily; Forkhead-associated (FHA) domains are small phosphopeptide recognition modules mostly found in eubacteria and eukaryotes. It is about 95-120 residues long that fold into an 11-stranded beta-sandwich. FHA domains can mediate the recognition of phosphorylated and non-phosphorylated substrates, as well as protein oligomerization. They specifically recognize threonine phosphorylation (pThr) accompanying activation of protein serine/threonine kinases. FHA domains show diverse ligand specificity. They may recognize the pTXXD motif, the pTXXI/L motif, and TQ clusters (singly and multiply phosphorylated). In eukaryotes, FHA superfamily members include forkhead-type transcription factors, as well as other signaling proteins, such as many regulatory proteins, kinases, phosphatases, motor proteins called kinesins, and metabolic enzymes. Many of them localize to the nucleus, where they participate in establishing or maintaining cell cycle checkpoints, DNA repair, or transcriptional regulation. FHA domains play important roles in human diseases, particularly in relation to DNA damage responses and cancers. In bacteria, FHA domain-containing proteins may participate in injection of viral proteins into host cells, transmembrane transporters, and cell division. FHA domain-containing proteins rarely include more than one copy of the domain. The only exception in eukaryotes is the checkpoint kinase Rad53 from Saccharomyces cerevisiae, which harbors two FHA domains (FHA1 and FHA2) flanking a central kinase domain. The two FHA domains recognize different phosphorylated targets and function independently from one another. In contrast, Mycobacterium tuberculosis ABC transporter Rv1747 contains two FHA domains but only one of them is essential for protein function.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1062670006 471 RYLMRSKEITFGReAKECgvdvDLSLEGPaaKISRRQGTIKmRSNGDFFIANEGKRA-IFIDGTPLlnGNKTRLAHNCTV 549
Cdd:cd00060    13 EFPLTKGVVTIGR-SPDC----DIVLDDP--SVSRRHARIE-VDGGGVYLEDLGSTNgTFVNGKRI--TPPVPLQDGDVI 82

                  ....*....
gi 1062670006 550 EISGLRFTF 558
Cdd:cd00060    83 RLGDTTFRF 91

forkhead associated (FHA) domain found in proliferation marker protein Ki-67 and similar proteins; Ki-67, also called antigen identified by monoclonal antibody Ki-67, antigen KI-67, or antigen Ki67, acts as a biological surfactant to disperse mitotic chromosomes. It is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Ki-67 binds DNA with a preference for supercoiled DNA and AT-rich DNA. It may also play a role in chromatin organization. Ki-67 contains an FHA domain at its N-terminus. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1062670006 471 RYLMRSKEITFGREAkECgvDVDLSLEGpaakISRRQGTIKMRSNGDFFIANEGK-RAIFIDGTPLLngNKTRLAHNCTV 549
Cdd:cd22673    15 RFPLTKKSCTFGRDL-SC--DIRIQLPG----VSREHCRIEVDENGKAYLENLSTtNPTLVNGKAIE--KSAELKDGDVI 85

                  ....*....
gi 1062670006 550 EISGLRFTF 558
Cdd:cd22673    86 TIGGRSFRF 94

forkhead associated (FHA) domain found in the Forkhead box protein K (FOXK) subfamily; The FOXK subfamily includes two-winged helix transcription factors, FOXK1 and FOXK2. FOXK1, also called myocyte nuclear factor (MNF), acts as a transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin genes. It positively regulates Wnt/beta-catenin signaling by translocating dishevelled (DVL) proteins into the nucleus. It also reduces virus replication, probably by binding the interferon stimulated response element (ISRE) to promote antiviral gene expression. In addition, FOXK1 plays important roles in multiple human cancers. FOXK2, also called cellular transcription factor ILF-1 or interleukin enhancer-binding factor 1, is a transcriptional regulator that recognizes the core sequence 5'-TAAACA-3'. It binds to NFAT-like motifs (purine-rich) in the IL2 promoter. It also binds to the HIV-1 long terminal repeat. FOXK2 may be involved in both positive and negative regulation of important viral and cellular promoter elements. In addition, FOXK2 plays a critical role in suppressing tumorigenesis. The Forkhead (FH) domain is a winged helix DNA-binding domain. FOX transcription factors recognize the core sequence 5'-(A/C)AA(C/T)A-3'. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1062670006 462 LACLCGRLVRYLMRSKEITFGREAKECGVDVDLsleGPAAKISRRQGTIKMrSNGDFFIANEGKRAIFIDGT 533
Cdd:cd22688     4 IARLEGREFEYLMRKSRVVIGRNSSRGPVDVNM---GHSSFISRKHLEIFY-EGGRFFLLCNGKNGIFVDGV 71

Forkhead associated domain; Found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 1062670006  479 ITFGREAKECgvDVDLSLEGpaakISRRQGTIKMRSNGDFFIANEGKRA-IFIDGTPL 535
Cdd:smart00240   1 VTIGRSSEDC--DIQLDGPS----ISRRHAVIVYDGGGRFYLIDLGSTNgTFVNGKRI 52