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Conserved domains on  [gi|802540666|ref|XP_012077692|]
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bifunctional bis(5'-adenosyl)-triphosphatase/adenylylsulfatase FHIT [Jatropha curcas]

Protein Classification

HIT family protein( domain architecture ID 10101080)

HIT (Histidine triad) family protein, named for a motif related to the sequence HxHxH/Qxx (x, a hydrophobic amino acid), is part of a superfamily of nucleotide hydrolases and transferases, which act on the alpha-phosphate of ribonucleotides; similar to Homo sapiens Bis(5'-adenosyl)-triphosphatase (also called fragile histidine triad protein) that cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP

CATH:  3.30.428.10
Gene Ontology:  GO:0003824
PubMed:  1472710
SCOP:  4000950

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FHIT cd01275
FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH ...
 52-172 2.32e-49

FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH/Qxx (x, is a hydrophobic amino acid), On the basis of sequence, substrate specificity, structure, evolution and mechanism, HIT proteins are classified into three branches: the Hint branch, which consists of adenosine 5' -monophosphoramide hydrolases, the Fhit branch, that consists of diadenosine polyphosphate hydrolases, and the GalT branch consisting of specific nucloside monophosphate transferases. Fhit plays a very important role in the development of tumours. Infact, Fhit deletions are among the earliest and most frequent genetic alterations in the development of tumours.


:

Pssm-ID: 238606 [Multi-domain]  Cd Length: 126  Bit Score: 156.68  E-value: 2.32e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 802540666  52 YKFGPYKIDPKE---VFYSTSLSYALVNLRPVVPGHVLVCPRREVKRFAELTADETSDLWLAAQKIGSRLESHHKATSLT 128
Cdd:cd01275    1 CVFCDIPIKPDEdnlVFYRTKHSFAVVNLYPYNPGHVLVVPYRHVPRLEDLTPEEIADLFKLVQLAMKALKVVYKPDGFN 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 802540666 129 LTIQDGPQAGQTVPHVHIHILPRKGGDFENND-EIYDAIDVKEKE 172
Cdd:cd01275   81 IGINDGKAGGGIVPHVHIHIVPRWNGDTNFMPvIIYTKVLPEDLE 125
 
Name Accession Description Interval E-value
FHIT cd01275
FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH ...
 52-172 2.32e-49

FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH/Qxx (x, is a hydrophobic amino acid), On the basis of sequence, substrate specificity, structure, evolution and mechanism, HIT proteins are classified into three branches: the Hint branch, which consists of adenosine 5' -monophosphoramide hydrolases, the Fhit branch, that consists of diadenosine polyphosphate hydrolases, and the GalT branch consisting of specific nucloside monophosphate transferases. Fhit plays a very important role in the development of tumours. Infact, Fhit deletions are among the earliest and most frequent genetic alterations in the development of tumours.


Pssm-ID: 238606 [Multi-domain]  Cd Length: 126  Bit Score: 156.68  E-value: 2.32e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 802540666  52 YKFGPYKIDPKE---VFYSTSLSYALVNLRPVVPGHVLVCPRREVKRFAELTADETSDLWLAAQKIGSRLESHHKATSLT 128
Cdd:cd01275    1 CVFCDIPIKPDEdnlVFYRTKHSFAVVNLYPYNPGHVLVVPYRHVPRLEDLTPEEIADLFKLVQLAMKALKVVYKPDGFN 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 802540666 129 LTIQDGPQAGQTVPHVHIHILPRKGGDFENND-EIYDAIDVKEKE 172
Cdd:cd01275   81 IGINDGKAGGGIVPHVHIHIVPRWNGDTNFMPvIIYTKVLPEDLE 125
HinT COG0537
Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide ...
 61-177 1.97e-29

Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide transport and metabolism, General function prediction only];


Pssm-ID: 440303 [Multi-domain]  Cd Length: 133  Bit Score: 105.80  E-value: 1.97e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 802540666  61 PKEVFYSTSLSYALVNLRPVVPGHVLVCPRREVKRFAELTADETSDLWLAAQKIGSRLESHHKATSLTLTIQDGPQAGQT 140
Cdd:COG0537   14 PALIVYEDEHVLAFLDINPYAPGHTLVIPKRHVASLFDLTPEELAELMRLAQKVAKALRKALGPDGFNLGINNGEAAGQT 93

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 802540666 141 VPHVHIHILPRKGGDFENNDEIYDAIDVKE-KELKQKL 177
Cdd:COG0537   94 VPHLHVHVIPRYEGDDNFMPVIGTKVDPEElEETARKL 131
HIT pfam01230
HIT domain;
61-154 2.86e-29

HIT domain;


Pssm-ID: 395984 [Multi-domain]  Cd Length: 98  Bit Score: 104.31  E-value: 2.86e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 802540666   61 PKEVFYSTSLSYALVNLRPVVPGHVLVCPRREVKRFAELTADETSDLWLAAQKIGSRLESHHKATSLTLTIQDGPQAGQT 140
Cdd:pfam01230   5 PSTVVYEDDLVLAFLDIDPQAPGHILVIPKKHIRELHDLTPEELGDLMSVAQKVARALGKVFKADGYRIVINNGAHAGQS 84

                          90
                  ....*....|....
gi 802540666  141 VPHVHIHILPRKGG 154
Cdd:pfam01230  85 VPHLHIHVIPRRKH 98
 
Name Accession Description Interval E-value
FHIT cd01275
FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH ...
 52-172 2.32e-49

FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH/Qxx (x, is a hydrophobic amino acid), On the basis of sequence, substrate specificity, structure, evolution and mechanism, HIT proteins are classified into three branches: the Hint branch, which consists of adenosine 5' -monophosphoramide hydrolases, the Fhit branch, that consists of diadenosine polyphosphate hydrolases, and the GalT branch consisting of specific nucloside monophosphate transferases. Fhit plays a very important role in the development of tumours. Infact, Fhit deletions are among the earliest and most frequent genetic alterations in the development of tumours.


Pssm-ID: 238606 [Multi-domain]  Cd Length: 126  Bit Score: 156.68  E-value: 2.32e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 802540666  52 YKFGPYKIDPKE---VFYSTSLSYALVNLRPVVPGHVLVCPRREVKRFAELTADETSDLWLAAQKIGSRLESHHKATSLT 128
Cdd:cd01275    1 CVFCDIPIKPDEdnlVFYRTKHSFAVVNLYPYNPGHVLVVPYRHVPRLEDLTPEEIADLFKLVQLAMKALKVVYKPDGFN 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 802540666 129 LTIQDGPQAGQTVPHVHIHILPRKGGDFENND-EIYDAIDVKEKE 172
Cdd:cd01275   81 IGINDGKAGGGIVPHVHIHIVPRWNGDTNFMPvIIYTKVLPEDLE 125
HinT COG0537
Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide ...
 61-177 1.97e-29

Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide transport and metabolism, General function prediction only];


Pssm-ID: 440303 [Multi-domain]  Cd Length: 133  Bit Score: 105.80  E-value: 1.97e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 802540666  61 PKEVFYSTSLSYALVNLRPVVPGHVLVCPRREVKRFAELTADETSDLWLAAQKIGSRLESHHKATSLTLTIQDGPQAGQT 140
Cdd:COG0537   14 PALIVYEDEHVLAFLDINPYAPGHTLVIPKRHVASLFDLTPEELAELMRLAQKVAKALRKALGPDGFNLGINNGEAAGQT 93

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 802540666 141 VPHVHIHILPRKGGDFENNDEIYDAIDVKE-KELKQKL 177
Cdd:COG0537   94 VPHLHVHVIPRYEGDDNFMPVIGTKVDPEElEETARKL 131
HIT pfam01230
HIT domain;
61-154 2.86e-29

HIT domain;


Pssm-ID: 395984 [Multi-domain]  Cd Length: 98  Bit Score: 104.31  E-value: 2.86e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 802540666   61 PKEVFYSTSLSYALVNLRPVVPGHVLVCPRREVKRFAELTADETSDLWLAAQKIGSRLESHHKATSLTLTIQDGPQAGQT 140
Cdd:pfam01230   5 PSTVVYEDDLVLAFLDIDPQAPGHILVIPKKHIRELHDLTPEELGDLMSVAQKVARALGKVFKADGYRIVINNGAHAGQS 84

                          90
                  ....*....|....
gi 802540666  141 VPHVHIHILPRKGG 154
Cdd:pfam01230  85 VPHLHIHVIPRRKH 98
HIT_like cd00468
HIT family: HIT (Histidine triad) proteins, named for a motif related to the sequence HxHxH ...
 65-150 5.62e-29

HIT family: HIT (Histidine triad) proteins, named for a motif related to the sequence HxHxH/Qxx (x, a hydrophobic amino acid), are a superfamily of nucleotide hydrolases and transferases, which act on the alpha-phosphate of ribonucleotides. On the basis of sequence, substrate specificity, structure, evolution and mechanism, HIT proteins are classified in the literacture into three major branches: the Hint branch, which consists of adenosine 5' -monophosphoramide hydrolases, the Fhit branch, that consists of diadenosine polyphosphate hydrolases, and the GalT branch consisting of specific nucloside monophosphate transferases. Further sequence analysis reveals several new closely related, yet uncharacterized subgroups.


Pssm-ID: 238263 [Multi-domain]  Cd Length: 86  Bit Score: 103.32  E-value: 5.62e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 802540666  65 FYSTSLSYALVNLRPVVPGHVLVCPRREVKRFAELTADETSDLWLAAQKIGSRLESHHKATSLTLTIQDGPQAGQTVPHV 144
Cdd:cd00468    1 VPDDEHSFAFVNLKPAAPGHVLVCPKRHVETLPDLDEALLADLVITAQRVAAELEKHGNVPSLTVFVNDGAAAGQSVPHV 80


                 ....*.
gi 802540666 145 HIHILP 150
Cdd:cd00468   81 HLHVLP 86
HINT_subgroup cd01277
HINT (histidine triad nucleotide-binding protein) subgroup: Members of this CD belong to the ...
 60-151 4.82e-20

HINT (histidine triad nucleotide-binding protein) subgroup: Members of this CD belong to the superfamily of histidine triad hydrolases that act on alpha-phosphate of ribonucleotides. This subgroup includes members from all three forms of cellular life. Although the biochemical function has not been characterised for many of the members of this subgroup, the proteins from Yeast have been shown to be involved in secretion, peroxisome formation and gene expression.


Pssm-ID: 238608 [Multi-domain]  Cd Length: 103  Bit Score: 80.73  E-value: 4.82e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 802540666  60 DPKEVFYSTSLSYALVNLRPVVPGHVLVCPRREVKRFAELTADETSDLWLAAQKIGSRLESHHKATSLTLTIQDGPQAGQ 139
Cdd:cd01277   12 IPSYKVYEDDHVLAFLDINPASKGHTLVIPKKHYENLLDLDPEELAELILAAKKVARALKKALKADGLNILQNNGRAAGQ 91

                         90
                 ....*....|..
gi 802540666 140 TVPHVHIHILPR 151
Cdd:cd01277   92 VVFHVHVHVIPR 103
PKCI_related cd01276
Protein Kinase C Interacting protein related (PKCI): PKCI and related proteins belong to the ...
 61-149 2.43e-11

Protein Kinase C Interacting protein related (PKCI): PKCI and related proteins belong to the ubiquitous HIT family of hydrolases that act on alpha-phosphates of ribonucleotides. The members of this subgroup have a conserved HxHxHxx motif (x is a hydrophobic residue) that is a signature for this family. No enzymatic activity has been reported however, for PKCI and its related members.


Pssm-ID: 238607 [Multi-domain]  Cd Length: 104  Bit Score: 57.96  E-value: 2.43e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 802540666  61 PKEVFYSTSLSYALVNLRPVVPGHVLVCPRREVKRFAELTAD---ETSDLWLAAQKIGSRLESHHKatSLTLTIQDGPQA 137
Cdd:cd01276   13 PAKKVYEDDEVLAFHDINPQAPVHILVIPKKHIASLSDATEEdeeLLGHLLSAAAKVAKDLGIAED--GYRLVINCGKDG 90

                         90
                 ....*....|..
gi 802540666 138 GQTVPHVHIHIL 149
Cdd:cd01276   91 GQEVFHLHLHLL 102
GalT COG1085
Galactose-1-phosphate uridylyltransferase [Carbohydrate transport and metabolism];
79-151 1.12e-04

Galactose-1-phosphate uridylyltransferase [Carbohydrate transport and metabolism];


Pssm-ID: 440702 [Multi-domain]  Cd Length: 336  Bit Score: 41.74  E-value: 1.12e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 802540666  79 PVVPGHVLVCPRREVKRFAELTADETSDLWLAAQKIGSRLESHHKATSLTLTIQDGPQAGQTVP--HVHIHILPR 151
Cdd:COG1085  223 ARWPFETWILPKRHVSDFEELTDEERDDLARILKRVLRRLDNLLGDFPYNMGLHQAPVDGEERDhyHWHLEIYPR 297
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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