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Conserved domains on  [gi|758988827|ref|XP_011393260|]
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RNA methylase, variant [Neurospora crassa OR74A]

Protein Classification

trimethylguanosine synthase family protein( domain architecture ID 10559581)

trimethylguanosine synthase catalyzes the two serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure

CATH:  2.20.25.110
EC:  2.1.1.-
Gene Ontology:  GO:0008757|GO:1904047|GO:0001510|GO:0008168
PubMed:  15590684|12504684|12826405
SCOP:  3000118

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Methyltransf_15 pfam09445
RNA cap guanine-N2 methyltransferase; RNA cap guanine-N2 methyltransferases such as ...
 82-298 1.10e-61

RNA cap guanine-N2 methyltransferase; RNA cap guanine-N2 methyltransferases such as Schizosaccharomyces pombe Tgs1 and Giardia lamblia Tgs2 catalyze methylation of the exocyclic N2 amine of 7-methylguanosine.


:

Pssm-ID: 370496  Cd Length: 165  Bit Score: 195.63  E-value: 1.10e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 758988827   82 RVLIDLFGGAGGNVIAFALSsgrWDRIIAIEKDRSTLACAQHNAEVYDVLDKITWVHGDCFEvmkrfwkttrgtkgmemk 161
Cdd:pfam09445   2 TRILDVFCGGGGNTIQFANV---FDSVISIDINLEHLACAQHNAEVYGVSDRIWLIHGDWFE------------------ 60

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 758988827  162 grgreggeaankgqeqeddneeldgLLAGLKLEEC---LVFASPPWGGVSYRDQEVFDL-SKMEPYNLEQLYKActmpqe 237
Cdd:pfam09445  61 -------------------------LLAKLKFEKIkydCVFASPPWGGPSYKKQNVYDLeNKLQPYGLSQLLKE------ 109

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 758988827  238 akeehpSTQKQKHILpqaLFLPRQSDLNQIAAL---VPEGAPKIDVVQYCQKGASKALVAYLPG 298
Cdd:pfam09445 110 ------FTKISKNVI---LFLPRNSDLNQLSALtreVLGPEAKCKVLYIKENGYMKGLLCYFGE 164
 
Name Accession Description Interval E-value
Methyltransf_15 pfam09445
RNA cap guanine-N2 methyltransferase; RNA cap guanine-N2 methyltransferases such as ...
 82-298 1.10e-61

RNA cap guanine-N2 methyltransferase; RNA cap guanine-N2 methyltransferases such as Schizosaccharomyces pombe Tgs1 and Giardia lamblia Tgs2 catalyze methylation of the exocyclic N2 amine of 7-methylguanosine.


Pssm-ID: 370496  Cd Length: 165  Bit Score: 195.63  E-value: 1.10e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 758988827   82 RVLIDLFGGAGGNVIAFALSsgrWDRIIAIEKDRSTLACAQHNAEVYDVLDKITWVHGDCFEvmkrfwkttrgtkgmemk 161
Cdd:pfam09445   2 TRILDVFCGGGGNTIQFANV---FDSVISIDINLEHLACAQHNAEVYGVSDRIWLIHGDWFE------------------ 60

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 758988827  162 grgreggeaankgqeqeddneeldgLLAGLKLEEC---LVFASPPWGGVSYRDQEVFDL-SKMEPYNLEQLYKActmpqe 237
Cdd:pfam09445  61 -------------------------LLAKLKFEKIkydCVFASPPWGGPSYKKQNVYDLeNKLQPYGLSQLLKE------ 109

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 758988827  238 akeehpSTQKQKHILpqaLFLPRQSDLNQIAAL---VPEGAPKIDVVQYCQKGASKALVAYLPG 298
Cdd:pfam09445 110 ------FTKISKNVI---LFLPRNSDLNQLSALtreVLGPEAKCKVLYIKENGYMKGLLCYFGE 164
TrmA COG2265
tRNA/tmRNA/rRNA uracil-C5-methylase, TrmA/RlmC/RlmD family [Translation, ribosomal structure ...
 80-147 7.10e-07

tRNA/tmRNA/rRNA uracil-C5-methylase, TrmA/RlmC/RlmD family [Translation, ribosomal structure and biogenesis]; tRNA/tmRNA/rRNA uracil-C5-methylase, TrmA/RlmC/RlmD family is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 441866 [Multi-domain]  Cd Length: 377  Bit Score: 50.56  E-value: 7.10e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 758988827  80 GKRVLIDLFGGAGGnvIAFALSSgRWDRIIAIEKDRSTLACAQHNAEVYDvLDKITWVHGDCFEVMKR 147
Cdd:COG2265  233 GGERVLDLYCGVGT--FALPLAR-RAKKVIGVEIVPEAVEDARENARLNG-LKNVEFVAGDLEEVLPE 296
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 82-144 5.31e-05

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 42.03  E-value: 5.31e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 758988827  82 RVLiDLFGGAGGNVIAFALSSGrwDRIIAIEKDRSTLACAQHNAEvYDVLDKITWVHGDCFEV 144
Cdd:cd02440    1 RVL-DLGCGTGALALALASGPG--ARVTGVDISPVALELARKAAA-ALLADNVEVLKGDAEEL 59
 
Name Accession Description Interval E-value
Methyltransf_15 pfam09445
RNA cap guanine-N2 methyltransferase; RNA cap guanine-N2 methyltransferases such as ...
 82-298 1.10e-61

RNA cap guanine-N2 methyltransferase; RNA cap guanine-N2 methyltransferases such as Schizosaccharomyces pombe Tgs1 and Giardia lamblia Tgs2 catalyze methylation of the exocyclic N2 amine of 7-methylguanosine.


Pssm-ID: 370496  Cd Length: 165  Bit Score: 195.63  E-value: 1.10e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 758988827   82 RVLIDLFGGAGGNVIAFALSsgrWDRIIAIEKDRSTLACAQHNAEVYDVLDKITWVHGDCFEvmkrfwkttrgtkgmemk 161
Cdd:pfam09445   2 TRILDVFCGGGGNTIQFANV---FDSVISIDINLEHLACAQHNAEVYGVSDRIWLIHGDWFE------------------ 60

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 758988827  162 grgreggeaankgqeqeddneeldgLLAGLKLEEC---LVFASPPWGGVSYRDQEVFDL-SKMEPYNLEQLYKActmpqe 237
Cdd:pfam09445  61 -------------------------LLAKLKFEKIkydCVFASPPWGGPSYKKQNVYDLeNKLQPYGLSQLLKE------ 109

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 758988827  238 akeehpSTQKQKHILpqaLFLPRQSDLNQIAAL---VPEGAPKIDVVQYCQKGASKALVAYLPG 298
Cdd:pfam09445 110 ------FTKISKNVI---LFLPRNSDLNQLSALtreVLGPEAKCKVLYIKENGYMKGLLCYFGE 164
TrmA COG2265
tRNA/tmRNA/rRNA uracil-C5-methylase, TrmA/RlmC/RlmD family [Translation, ribosomal structure ...
 80-147 7.10e-07

tRNA/tmRNA/rRNA uracil-C5-methylase, TrmA/RlmC/RlmD family [Translation, ribosomal structure and biogenesis]; tRNA/tmRNA/rRNA uracil-C5-methylase, TrmA/RlmC/RlmD family is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 441866 [Multi-domain]  Cd Length: 377  Bit Score: 50.56  E-value: 7.10e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 758988827  80 GKRVLIDLFGGAGGnvIAFALSSgRWDRIIAIEKDRSTLACAQHNAEVYDvLDKITWVHGDCFEVMKR 147
Cdd:COG2265  233 GGERVLDLYCGVGT--FALPLAR-RAKKVIGVEIVPEAVEDARENARLNG-LKNVEFVAGDLEEVLPE 296
HemK COG2890
Methylase of polypeptide chain release factors [Translation, ribosomal structure and ...
 72-143 1.24e-05

Methylase of polypeptide chain release factors [Translation, ribosomal structure and biogenesis];


Pssm-ID: 442135 [Multi-domain]  Cd Length: 282  Bit Score: 46.30  E-value: 1.24e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 758988827  72 LSTHPIADGKRVLiDLfgGAGGNVIAFALSSGRWD-RIIAIEKDRSTLACAQHNAEVYDVLDKITWVHGDCFE 143
Cdd:COG2890  105 LALLPAGAPPRVL-DL--GTGSGAIALALAKERPDaRVTAVDISPDALAVARRNAERLGLEDRVRFLQGDLFE 174
Trm5 COG2520
tRNA G37 N-methylase Trm5 [Translation, ribosomal structure and biogenesis]; tRNA G37 ...
 67-148 2.16e-05

tRNA G37 N-methylase Trm5 [Translation, ribosomal structure and biogenesis]; tRNA G37 N-methylase Trm5 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 442010 [Multi-domain]  Cd Length: 333  Bit Score: 46.01  E-value: 2.16e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 758988827  67 RIARDlsthpIADGKRVLiDLFGGAGGNVIAFALSSGRwdRIIAIEKDRSTLACAQHNAEVYDVLDKITWVHGDCFEVMK 146
Cdd:COG2520  173 RIAEL-----VKPGERVL-DMFAGVGPFSIPIAKRSGA--KVVAIDINPDAVEYLKENIRLNKVEDRVTPILGDAREVAP 244


                 ..
gi 758988827 147 RF 148
Cdd:COG2520  245 EL 246
RlmK COG1092
23S rRNA G2069 N7-methylase RlmK or C1962 C5-methylase RlmI [Translation, ribosomal structure ...
 76-148 3.78e-05

23S rRNA G2069 N7-methylase RlmK or C1962 C5-methylase RlmI [Translation, ribosomal structure and biogenesis]; 23S rRNA G2069 N7-methylase RlmK or C1962 C5-methylase RlmI is part of the Pathway/BioSystem: 23S rRNA modification


Pssm-ID: 440709 [Multi-domain]  Cd Length: 392  Bit Score: 45.17  E-value: 3.78e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 758988827  76 PIADGKRVLiDLF---GGAGgnviAFALSSGRwDRIIAIEKDRSTLACAQHNAEVYDVLDKITWVHGDCFEVMKRF 148
Cdd:COG1092  213 ELAKGKRVL-NLFsytGGFS----VHAAAGGA-KSVTSVDLSATALEWAKENAALNGLDDRHEFVQADAFDWLREL 282
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 82-144 5.31e-05

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 42.03  E-value: 5.31e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 758988827  82 RVLiDLFGGAGGNVIAFALSSGrwDRIIAIEKDRSTLACAQHNAEvYDVLDKITWVHGDCFEV 144
Cdd:cd02440    1 RVL-DLGCGTGALALALASGPG--ARVTGVDISPVALELARKAAA-ALLADNVEVLKGDAEEL 59
COG4076 COG4076
Predicted RNA methylase [General function prediction only];
77-144 1.33e-03

Predicted RNA methylase [General function prediction only];


Pssm-ID: 443253 [Multi-domain]  Cd Length: 230  Bit Score: 40.02  E-value: 1.33e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 758988827  77 IADGKRVLiDLfgGAGGNVIA-FALSSGRwDRIIAIEKDRSTLACAQHNAEVYDVLDKITWVHGDCFEV 144
Cdd:COG4076   33 VKPGDVVL-DI--GTGSGLLSmLAARAGA-KKVYAVEVNPDIAAVARRIIAANGLSDRITVINADATDL 97
TrmN6 COG4123
tRNA1(Val) A37 N6-methylase TrmN6 [Translation, ribosomal structure and biogenesis]; tRNA1(Val) ...
 76-140 5.07e-03

tRNA1(Val) A37 N6-methylase TrmN6 [Translation, ribosomal structure and biogenesis]; tRNA1(Val) A37 N6-methylase TrmN6 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 443299 [Multi-domain]  Cd Length: 238  Bit Score: 38.20  E-value: 5.07e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 758988827  76 PIADGKRVLiDLfgGAGGNVIAFALSSgRWD--RIIAIEKDRSTLACAQHNAEVYDVLDKITWVHGD 140
Cdd:COG4123   34 PVKKGGRVL-DL--GTGTGVIALMLAQ-RSPgaRITGVEIQPEAAELARRNVALNGLEDRITVIHGD 96
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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