|
Name |
Accession |
Description |
Interval |
E-value |
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
55-576 |
0e+00 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 673.23 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADQHGSHipagshTYRDLYSRSLSLAREICRLracsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRK 134
Cdd:cd05941 1 DRIAIVDDGDSI------TYADLVARAARLANRLLAL----GKDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPS 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 135 HPQAELEYFIQDSRSSVVLagqehvellspvvrklgvpllplpptvycgaakdpgeeqvperdwrdRGAMIIYTSGTTGR 214
Cdd:cd05941 71 YPLAELEYVITDSEPSLVL-----------------------------------------------DPALILYTSGTTGR 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 215 PKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWekfLSSQSPRVN 294
Cdd:cd05941 104 PKGVVLTHANLAANVRALVDAWRWTEDDVLLHVLPLHHVHGLVNALLCPLFAGASVEFLPKFDPKEVA---ISRLMPSIT 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 295 VFMAVPTIYSKLMDYHDRHFGQPhvrDFVRAVCEEKIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIGMALSNPL 374
Cdd:cd05941 181 VFMGVPTIYTRLLQYYEAHFTDP---QFARAAAAERLRLMVSGSAALPVPTLEEWEAITGHTLLERYGMTEIGMALSNPL 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 375 ATARLPGSVGTPLPGVEVRIVSENPQKdgcpyiihaegnetetrvtPGFEETEGELLVRGPSVFRGYWDKPEETKNAFTS 454
Cdd:cd05941 258 DGERRPGTVGMPLPGVQARIVDEETGE-------------------PLPRGEVGEIQVRGPSVFKEYWNKPEATKEEFTD 318
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 455 DGWFKTGDTAVFK-DGSYWIRGRTSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREG-HS 532
Cdd:cd05941 319 DGWFKTGDLGVVDeDGYYWILGRSSVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRAGaAA 398
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 743747175 533 LSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQ 576
Cdd:cd05941 399 LSLEELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
49-578 |
1.18e-142 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 420.76 E-value: 1.18e-142
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALADQhgshipAGSHTYRDLYSRSLSLAReicRLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:COG0318 8 AAARHPDRPALVFG------GRRLTYAELDARARRLAA---ALRAL--GVGPGDRVALLLPNSPEFVVAFLAALRAGAVV 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLYRKHPQAELEYFIQDSRSSVVLAgqehvellspvvrklgvpllplpptvycgaakdpgeeqvperdwrdrgAMIIYT 208
Cdd:COG0318 77 VPLNPRLTAEELAYILEDSGARALVT------------------------------------------------ALILYT 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 209 SGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWEKFlss 288
Cdd:COG0318 109 SGTTGRPKGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPLLAGATLVLLPRFDPERVLELI--- 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 289 QSPRVNVFMAVPTIYSKLMDYHDRHFgqphvRDFvravceEKIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIGM 368
Cdd:COG0318 186 ERERVTVLFGVPTMLARLLRHPEFAR-----YDL------SSLRLVVSGGAPLPPELLERFEERFGVRIVEGYGLTETSP 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 369 ALS-NPL-ATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGNEtetrVTPGfeeTEGELLVRGPSVFRGYWDKPE 446
Cdd:COG0318 255 VVTvNPEdPGERRPGSVGRPLPGVEVRIVDE-------------DGRE----LPPG---EVGEIVVRGPNVMKGYWNDPE 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 447 ETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVV 525
Cdd:COG0318 315 ATAEAF-RDGWLRTGDLGRLdEDGYLYIVGRKK-DMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFV 392
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 743747175 526 TLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:COG0318 393 VLRPGAELDAEELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRERY 445
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
46-575 |
9.12e-115 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 349.55 E-value: 9.12e-115
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 46 VFTRALA-FGDRVALADqHGSHIpagshTYRDLYSRSLSLAReicRLRA--CSDGDlreeRVSFLCSNDVSYVVAQWASW 122
Cdd:cd05936 4 LLEEAARrFPDKTALIF-MGRKL-----TYRELDALAEAFAA---GLQNlgVQPGD----RVALMLPNCPQFPIAYFGAL 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 123 MSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLspvvrklgvpllplpptvycgaAKDPGEEQVPERDwRDRG 202
Cdd:cd05936 71 KAGAVVVPLNPLYTPRELEHILNDSGAKALIVAVSFTDLL----------------------AAGAPLGERVALT-PEDV 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLvhkWAWTK-----DDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFS 277
Cdd:cd05936 128 AVLQYTSGTTGVPKGAMLTHRNLVANALQI---KAWLEdllegDDVVLAALPLFHVFGLTVALLLPLALGATIVLIPRFR 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 278 AQLVWEKFlssQSPRVNVFMAVPTIYSKLMDYHDRHFgqphvRDFvravceEKIRLMVSGSAALPLPVLEKWKGATGHTL 357
Cdd:cd05936 205 PIGVLKEI---RKHRVTIFPGVPTMYIALLNAPEFKK-----RDF------SSLRLCISGGAPLPVEVAERFEELTGVPI 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 358 LERYGMTEIG-MALSNPLATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGNEtetrVTPGfeeTEGELLVRGPS 436
Cdd:cd05936 271 VEGYGLTETSpVVAVNPLDGPRKPGSIGIPLPGTEVKIVDD-------------DGEE----LPPG---EVGELWVRGPQ 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 437 VFRGYWDKPEETKNAFTsDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDV 515
Cdd:cd05936 331 VMKGYWNRPEETAEAFV-DGWLRTGDIGYMdEDGYFFIVDRKK-DMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDP 408
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 516 TWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLV 575
Cdd:cd05936 409 YSGEAVKAFVVLKEGASLTEEEIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
30-576 |
3.76e-110 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 337.73 E-value: 3.76e-110
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 30 SPLHTGPAARGNRSAPvftrALAFGDRVAladqhgshipagshTYRDLYSRSLSLAREICRLRacsdgdlreeRVSFLCS 109
Cdd:PRK07787 2 ASLNPAAVAAAADIAD----AVRIGGRVL--------------SRSDLAGAATAVAERVAGAR----------RVAVLAT 53
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 110 NDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVrklgvpllplppTVYCGAAKDPG 189
Cdd:PRK07787 54 PTLATVLAVVGALIAGVPVVPVPPDSGVAERRHILADSGAQAWLGPAPDDPAGLPHV------------PVRLHARSWHR 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 190 EEQVPErdwrDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGAT 269
Cdd:PRK07787 122 YPEPDP----DAPALIVYTSGTTGPPKGVVLSRRAIAADLDALAEAWQWTADDVLVHGLPLFHVHGLVLGVLGPLRIGNR 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 270 CVMLPEFSAqlvwEKFLSSQSPRVNVFMAVPTIYSKLMDYHDrhfgqpHVRDFVRAvceekiRLMVSGSAALPLPVLEKW 349
Cdd:PRK07787 198 FVHTGRPTP----EAYAQALSEGGTLYFGVPTVWSRIAADPE------AARALRGA------RLLVSGSAALPVPVFDRL 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 350 KGATGHTLLERYGMTEIGMALSNPLATARLPGSVGTPLPGVEVRIVSEnpqkDGCPyiIHAEGnetetrvtpgfeETEGE 429
Cdd:PRK07787 262 AALTGHRPVERYGMTETLITLSTRADGERRPGWVGLPLAGVETRLVDE----DGGP--VPHDG------------ETVGE 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 430 LLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFK-DGSYWIRGRTSVDIIKTGGYKVSALEVERLLLGHPSIADVA 508
Cdd:PRK07787 324 LQVRGPTLFDGYLNRPDATAAAFTADGWFRTGDVAVVDpDGMHRIVGRESTDLIKSGGYRIGAGEIETALLGHPGVREAA 403
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 743747175 509 VIGVPDVTWGQRVTAVVTLREGhsLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQ 576
Cdd:PRK07787 404 VVGVPDDDLGQRIVAYVVGADD--VAADELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLLS 469
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
43-577 |
1.41e-105 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 327.22 E-value: 1.41e-105
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 43 SAPVFTR---ALAFGDRVALADQHGSHIpagshTYRDLYSRSLSLAReicRLRACsdgDLRE-ERVSFLCSNDVSYVVAQ 118
Cdd:PRK07514 2 NNNLFDAlraAFADRDAPFIETPDGLRY-----TYGDLDAASARLAN---LLVAL---GVKPgDRVAVQVEKSPEALALY 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 119 WASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPLLPLPPTVYCG------AAKDPGEEQ 192
Cdd:PRK07514 71 LATLRAGAVFLPLNTAYTLAELDYFIGDAEPALVVCDPANFAWLSKIAAAAGAPHVETLDADGTGslleaaAAAPDDFET 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 193 VPERDwrDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVM 272
Cdd:PRK07514 151 VPRGA--DDLAAILYTSGTTGRSKGAMLSHGNLLSNALTLVDYWRFTPDDVLIHALPIFHTHGLFVATNVALLAGASMIF 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 273 LPEFSAQLVWEKFlssqsPRVNVFMAVPTIYSKLMDyHDRhfgqphvrdFVRAVCEeKIRLMVSGSAALPLPVLEKWKGA 352
Cdd:PRK07514 229 LPKFDPDAVLALM-----PRATVMMGVPTFYTRLLQ-EPR---------LTREAAA-HMRLFISGSAPLLAETHREFQER 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 353 TGHTLLERYGMTEIGMALSNPLATARLPGSVGTPLPGVEVRIVsenpqkdgcpyiihaeGNETETRVTPGfeETeGELLV 432
Cdd:PRK07514 293 TGHAILERYGMTETNMNTSNPYDGERRAGTVGFPLPGVSLRVT----------------DPETGAELPPG--EI-GMIEV 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 433 RGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRtSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIG 511
Cdd:PRK07514 354 KGPNVFKGYWRMPEKTAEEFRADGFFITGDLGKIdERGYVHIVGR-GKDLIISGGYNVYPKEVEGEIDELPGVVESAVIG 432
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 512 VPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQ 577
Cdd:PRK07514 433 VPHPDFGEGVTAVVVPKPGAALDEAAILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLLREQ 498
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
73-578 |
5.43e-104 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 323.68 E-value: 5.43e-104
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREicrLRAC--SDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTAVPL-YRKHPQaELEYFIQDSRS 149
Cdd:PRK06187 33 TYAELDERVNRLANA---LRALgvKKGD----RVAVFDWNSHEYLEAYFAVPKIGAVLHPInIRLKPE-EIAYILNDAED 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 150 SVVLAGQEHVELLSPVVRKLgvPLLPLPPTVYCGAAKDPGEE----------QVPERDWRDRG----AMIIYTSGTTGRP 215
Cdd:PRK06187 105 RVVLVDSEFVPLLAAILPQL--PTVRTVIVEGDGPAAPLAPEvgeyeellaaASDTFDFPDIDendaAAMLYTSGTTGHP 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 216 KGVVITHHNVWATVTGlVHKW-AWTKDDVILHVLPLHHVHG----VVnkllcPLWVGATCVMLPEFSAQLVWEKFLSSqs 290
Cdd:PRK06187 183 KGVVLSHRNLFLHSLA-VCAWlKLSRDDVYLVIVPMFHVHAwglpYL-----ALMAGAKQVIPRRFDPENLLDLIETE-- 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 291 pRVNVFMAVPTIYSKLMDYHDRHFgqphvRDFVRavceekIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIGMAL 370
Cdd:PRK06187 255 -RVTFFFAVPTIWQMLLKAPRAYF-----VDFSS------LRLVIYGGAALPPALLREFKEKFGIDLVQGYGMTETSPVV 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 371 S-NPL-----ATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGNEtetrVTPGFEETeGELLVRGPSVFRGYWDK 444
Cdd:PRK06187 323 SvLPPedqlpGQWTKRRSAGRPLPGVEARIVDD-------------DGDE----LPPDGGEV-GEIIVRGPWLMQGYWNR 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 445 PEETKNAFTsDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTA 523
Cdd:PRK06187 385 PEATAETID-GGWLHTGDVGYIdEDGYLYITDRIK-DVIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVA 462
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 743747175 524 VVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK06187 463 VVVLKPGATLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVLREQY 517
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
49-571 |
1.47e-103 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 319.56 E-value: 1.47e-103
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALADqhgshiPAGSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:cd17631 4 RARRHPDRTALVF------GGRSLTYAELDERVNRLAHALRAL-----GVAKGDRVAVLSKNSPEFLELLFAAARLGAVF 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPL-YRKHPqAELEYFIQDSRSSVVLagqehvellspvvrklgvpllplpptvycgaakdpgeeqvperdwrDRGAMIIY 207
Cdd:cd17631 73 VPLnFRLTP-PEVAYILADSGAKVLF----------------------------------------------DDLALLMY 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 208 TSGTTGRPKGVVITHHNV-WATVTGLVHkWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAqlvwEKFL 286
Cdd:cd17631 106 TSGTTGRPKGAMLTHRNLlWNAVNALAA-LDLGPDDVLLVVAPLFHIGGLGVFTLPTLLRGGTVVILRKFDP----ETVL 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 287 S-SQSPRVNVFMAVPTIYSKLMDyHDRhfgqPHVRDFVRavceekIRLMVSGSAALPLPVLEKWKgATGHTLLERYGMTE 365
Cdd:cd17631 181 DlIERHRVTSFFLVPTMIQALLQ-HPR----FATTDLSS------LRAVIYGGAPMPERLLRALQ-ARGVKFVQGYGMTE 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 366 IGMALS--NPLATARLPGSVGTPLPGVEVRIVSENpqkdgcpyiihaeGNEtetrVTPGfeeTEGELLVRGPSVFRGYWD 443
Cdd:cd17631 249 TSPGVTflSPEDHRRKLGSAGRPVFFVEVRIVDPD-------------GRE----VPPG---EVGEIVVRGPHVMAGYWN 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 444 KPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVT 522
Cdd:cd17631 309 RPEATAAAF-RDGWFHTGDLGRLdEDGYLYIVDRKK-DMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVV 386
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 743747175 523 AVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNK 571
Cdd:cd17631 387 AVVVPRPGAELDEDELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
203-569 |
6.01e-103 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 314.61 E-value: 6.01e-103
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVhGVVNKLLCPLWVGATCVMLPEFSAQLVW 282
Cdd:cd04433 3 ALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLPKFDPEAAL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 283 EKFlssQSPRVNVFMAVPTIYSKLMDyHDRHFGqphvRDFVRavceekIRLMVSGSAALPLPVLEKWKGATGHTLLERYG 362
Cdd:cd04433 82 ELI---EREKVTILLGVPTLLARLLK-APESAG----YDLSS------LRALVSGGAPLPPELLERFEEAPGIKLVNGYG 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 363 MTEIG--MALSNPLATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaegnetETRVTPGFEEteGELLVRGPSVFRG 440
Cdd:cd04433 148 LTETGgtVATGPPDDDARKPGSVGRPVPGVEVRIVDP------------------DGGELPPGEI--GELVVRGPSVMKG 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 441 YWDKPEETKnAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQ 519
Cdd:cd04433 208 YWNNPEATA-AVDEDGWYRTGDLGRLdEDGYLYIVGRLK-DMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGE 285
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|
gi 743747175 520 RVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKV 569
Cdd:cd04433 286 RVVAVVVLRPGADLDAEELRAHVRERLAPYKVPRRVVFVDALPRTASGKI 335
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
67-576 |
1.18e-98 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 308.86 E-value: 1.18e-98
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 67 IPAGSH--TYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFI 144
Cdd:cd05926 8 VPGSTPalTYADLAELVDDLARQLAAL-----GIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 145 QDSRSSVVLA----GQEHVEL---LSPVVRKLGVPLLPLPPTV------YCGAAKDPGEEQVPERdwRDRGAMIIYTSGT 211
Cdd:cd05926 83 ADLGSKLVLTpkgeLGPASRAaskLGLAILELALDVGVLIRAPsaeslsNLLADKKNAKSEGVPL--PDDLALILHTSGT 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 212 TGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWEKFlssQSP 291
Cdd:cd05926 161 TGRPKGVPLTHRNLAASATNITNTYKLTPDDRTLVVMPLFHVHGLVASLLSTLAAGGSVVLPPRFSASTFWPDV---RDY 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 292 RVNVFMAVPTIYSKLMDYHDRHFGQPHVrdfvravceeKIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIG--MA 369
Cdd:cd05926 238 NATWYTAVPTIHQILLNRPEPNPESPPP----------KLRFIRSCSASLPPAVLEALEATFGAPVLEAYGMTEAAhqMT 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 370 lSNPL-ATARLPGSVGTPLpGVEVRIVSEN--PQKDGcpyiihaegnetetrvtpgfeeTEGELLVRGPSVFRGYWDKPE 446
Cdd:cd05926 308 -SNPLpPGPRKPGSVGKPV-GVEVRILDEDgeILPPG----------------------VVGEICLRGPNVTRGYLNNPE 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 447 ETKNAFTSDGWFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVV 525
Cdd:cd05926 364 ANAEAAFKDGWFRTGDLGYLDaDGYLFLTGRIK-ELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAV 442
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 743747175 526 TLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQ 576
Cdd:cd05926 443 VLREGASVTEEELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
50-574 |
1.11e-90 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 288.73 E-value: 1.11e-90
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 50 ALAFGDRVALADQHGSHipagshTYRDLYSRSLSLAReicrlrACSD-----GDlreeRVSFLCSNDVSYVVAQWASWMS 124
Cdd:PRK07656 15 ARRFGDKEAYVFGDQRL------TYAELNARVRRAAA------ALAAlgigkGD----RVAIWAPNSPHWVIAALGALKA 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 125 GGTAVPLYRKHPQAELEYFIQDSRSSVVLAgqehVELLSPVVRKLGVPLLPLPPTVYC--GAAKDPGEEQVPERDWRDRG 202
Cdd:PRK07656 79 GAVVVPLNTRYTADEAAYILARGDAKALFV----LGLFLGVDYSATTRLPALEHVVICetEEDDPHTEKMKTFTDFLAAG 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 --------------AMIIYTSGTTGRPKGVVITHHN------VWATVTGLvhkwawTKDDVILHVLPLHHVHGVVNKLLC 262
Cdd:PRK07656 155 dpaerapevdpddvADILFTSGTTGRPKGAMLTHRQllsnaaDWAEYLGL------TEGDRYLAANPFFHVFGYKAGVNA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 263 PLWVGATCVMLPEFSAQLVwekFLSSQSPRVNVFMAVPTIYSKLMDYHDRHfgqphVRDFvravceEKIRLMVSGSAALP 342
Cdd:PRK07656 229 PLMRGATILPLPVFDPDEV---FRLIETERITVLPGPPTMYNSLLQHPDRS-----AEDL------SSLRLAVTGAASMP 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 343 LPVLEKWKGATG-HTLLERYGMTE-IGMALSNPLATAR--LPGSVGTPLPGVEVRIVSENpqkdgcpyiihaeGNETETR 418
Cdd:PRK07656 295 VALLERFESELGvDIVLTGYGLSEaSGVTTFNRLDDDRktVAGTIGTAIAGVENKIVNEL-------------GEEVPVG 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 419 VTpgfeeteGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERL 497
Cdd:PRK07656 362 EV-------GELLVRGPNVMKGYYDDPEATAAAIDADGWLHTGDLGRLdEEGYLYIVDRKK-DMFIVGGFNVYPAEVEEV 433
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 743747175 498 LLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK07656 434 LYEHPAVAEAAVIGVPDERLGEVGKAYVVLKPGAELTEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRAL 510
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
46-485 |
4.06e-88 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 279.20 E-value: 4.06e-88
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 46 VFTRALAFGDRVALADQHGSHIpagshTYRDLYSRSLSLAReicRLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSG 125
Cdd:pfam00501 1 LERQAARTPDKTALEVGEGRRL-----TYRELDERANRLAA---GLRAL--GVGKGDRVAILLPNSPEWVVAFLACLKAG 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 126 GTAVPLYRKHPQAELEYFIQDSRSSVVLAgqEHVELLSPVVRKLGVPLLPLPPTVY-------------CGAAKDPGEEQ 192
Cdd:pfam00501 71 AVYVPLNPRLPAEELAYILEDSGAKVLIT--DDALKLEELLEALGKLEVVKLVLVLdrdpvlkeeplpeEAKPADVPPPP 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 193 VPERDWRDRgAMIIYTSGTTGRPKGVVITHHNVWATVTGL----VHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGA 268
Cdd:pfam00501 149 PPPPDPDDL-AYIIYTSGTTGKPKGVMLTHRNLVANVLSIkrvrPRGFGLGPDDRVLSTLPLFHDFGLSLGLLGPLLAGA 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 269 TCVMLPEFSAQLVWEKFLSSQSPRVNVFMAVPTIYSKLMDYhdrhfGQPHVRDFVRavceekIRLMVSGSAALPLPVLEK 348
Cdd:pfam00501 228 TVVLPPGFPALDPAALLELIERYKVTVLYGVPTLLNMLLEA-----GAPKRALLSS------LRLVLSGGAPLPPELARR 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 349 WKGATGHTLLERYGMTEIGMALSNPLAT---ARLPGSVGTPLPGVEVRIVSENpqkdgcpyiihaEGNEtetrVTPGfee 425
Cdd:pfam00501 297 FRELFGGALVNGYGLTETTGVVTTPLPLdedLRSLGSVGRPLPGTEVKIVDDE------------TGEP----VPPG--- 357
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 743747175 426 TEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTG 485
Cdd:pfam00501 358 EPGELCVRGPGVMKGYLNDPELTAEAFDEDGWYRTGDLGRRdEDGYLEIVGRKK-DQIKLG 417
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
203-578 |
1.57e-86 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 279.96 E-value: 1.57e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATvtgLVHKWAWT-----KDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFS 277
Cdd:PRK05605 222 ALILYTSGTTGKPKGAQLTHRNLFAN---AAQGKAWVpglgdGPERVLAALPMFHAYGLTLCLTLAVSIGGELVLLPAPD 298
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 278 AQLVwekfLSSQSPRVNVFM-AVPTIYSKLMDYHDRHfgqphvrdfvrAVCEEKIRLMVSGSAALPLPVLEKWKGATGHT 356
Cdd:PRK05605 299 IDLI----LDAMKKHPPTWLpGVPPLYEKIAEAAEER-----------GVDLSGVRNAFSGAMALPVSTVELWEKLTGGL 363
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 357 LLERYGMTEIG-MALSNPLATARLPGSVGTPLPGVEVRIVseNPQkdgcpyiihaegNETETRvTPGfeeTEGELLVRGP 435
Cdd:PRK05605 364 LVEGYGLTETSpIIVGNPMSDDRRPGYVGVPFPDTEVRIV--DPE------------DPDETM-PDG---EEGELLVRGP 425
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 436 SVFRGYWDKPEETKNAFTsDGWFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPD 514
Cdd:PRK05605 426 QVFKGYWNRPEETAKSFL-DGWFRTGDVVVMEeDGFIRIVDRIK-ELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPR 503
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 743747175 515 VTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK05605 504 EDGSEEVVAAVVLEPGAALDPEGLRAYCREHLTRYKVPRRFYHVDELPRDQLGKVRRREVREEL 567
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
54-585 |
3.43e-86 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 278.53 E-value: 3.43e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 54 GDRVAL--ADQHGSHipaGSHTYRDLYSRSLSLAReicRLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVP- 130
Cdd:COG0365 23 GDKVALiwEGEDGEE---RTLTYAELRREVNRFAN---ALRAL--GVKKGDRVAIYLPNIPEAVIAMLACARIGAVHSPv 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 131 --LYRKHpqaELEYFIQDSRSSVVLA------GQEHVELL---------SP------VVRKLGVPLLPLPPTVYCGAAKD 187
Cdd:COG0365 95 fpGFGAE---ALADRIEDAEAKVLITadgglrGGKVIDLKekvdealeeLPslehviVVGRTGADVPMEGDLDWDELLAA 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 188 PGEEQVPERDWRDRGAMIIYTSGTTGRPKGVVITHHN--VWATVTglvHKWAW--TKDDVILHVLPLHHVHGVVNKLLCP 263
Cdd:COG0365 172 ASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGylVHAATT---AKYVLdlKPGDVFWCTADIGWATGHSYIVYGP 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 264 LWVGATCVML---PEF-SAQLVW---EKFlssqspRVNVFMAVPTIYSKLMDYHDRHFGQphvRDFVRavceekIRLMVS 336
Cdd:COG0365 249 LLNGATVVLYegrPDFpDPGRLWeliEKY------GVTVFFTAPTAIRALMKAGDEPLKK---YDLSS------LRLLGS 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 337 GSAALPLPVLEKWKGATGHTLLERYGMTEIGMALSNPLATARL-PGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGNEt 415
Cdd:COG0365 314 AGEPLNPEVWEWWYEAVGVPIVDGWGQTETGGIFISNLPGLPVkPGSMGKPVPGYDVAVVDE-------------DGNP- 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 416 etrVTPGfeeTEGELLVRG--PSVFRGYWDKPEETKNAF--TSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVS 490
Cdd:COG0365 380 ---VPPG---EEGELVIKGpwPGMFRGYWNDPERYRETYfgRFPGWYRTGDGARRdEDGYFWILGRSD-DVINVSGHRIG 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 491 ALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLS---HRELKEWARGVLAPYAVPSELLLVEEIPRNQMG 567
Cdd:COG0365 453 TAEIESALVSHPAVAEAAVVGVPDEIRGQVVKAFVVLKPGVEPSdelAKELQAHVREELGPYAYPREIEFVDELPKTRSG 532
|
570 580
....*....|....*....|.
gi 743747175 568 KVNK---RDLVQQLYPQDRGT 585
Cdd:COG0365 533 KIMRrllRKIAEGRPLGDTST 553
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
54-569 |
4.15e-85 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 274.25 E-value: 4.15e-85
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 54 GDRVALADqhgshiPAGSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYR 133
Cdd:cd05959 18 GDKTAFID------DAGSLTYAELEAEARRVAGALRAL-----GVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNT 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 134 KHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPLLPLPptVYCGAAKDPGEEQV------------PERDWRDR 201
Cdd:cd05959 87 LLTPDDYAYYLEDSRARVVVVSGELAPVLAAALTKSEHTLVVLI--VSGGAGPEAGALLLaelvaaeaeqlkPAATHADD 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 202 GAMIIYTSGTTGRPKGVVITHHNV-WATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEF-SAQ 279
Cdd:cd05959 165 PAFWLYSSGSTGRPKGVVHLHADIyWTAELYARNVLGIREDDVCFSAAKLFFAYGLGNSLTFPLSVGATTVLMPERpTPA 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 280 LVWEKFlssQSPRVNVFMAVPTIYSKLMDYHDrhfgqPHVRDFVRavceekIRLMVSGSAALPLPVLEKWKGATGHTLLE 359
Cdd:cd05959 245 AVFKRI---RRYRPTVFFGVPTLYAAMLAAPN-----LPSRDLSS------LRLCVSAGEALPAEVGERWKARFGLDILD 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 360 RYGMTEIG-MALSNpLATARLPGSVGTPLPGVEVRIVSENpqkdgcpyiihaeGNETEtrvtpgfEETEGELLVRGPSVF 438
Cdd:cd05959 311 GIGSTEMLhIFLSN-RPGRVRYGTTGKPVPGYEVELRDED-------------GGDVA-------DGEPGELYVRGPSSA 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 439 RGYWDKPEETKNAFTSdGWFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTW 517
Cdd:cd05959 370 TMYWNNRDKTRDTFQG-EWTRTGDKYVRDdDGFYTYAGRAD-DMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDG 447
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 743747175 518 GQRVTAVVTLREGHS---LSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKV 569
Cdd:cd05959 448 LTKPKAFVVLRPGYEdseALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKI 502
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
73-570 |
1.38e-84 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 272.16 E-value: 1.38e-84
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAreiCRLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:cd05911 12 TYAQLRTLSRRLA---AGLRK--LGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVI 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHVELLSPVVRKLGVPLLPLPPTV---YCGAAKDPGEEQVPERDWR---------DRGAMIIYTSGTTGRPKGVVI 220
Cdd:cd05911 87 FTDPDGLEKVKEAAKELGPKDKIIVLDDkpdGVLSIEDLLSPTLGEEDEDlppplkdgkDDTAAILYSSGTTGLPKGVCL 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 221 THHNV--WATVTGLVHKWAWTKDDVILHVLPLHHVHGVvNKLLCPLWVGATCVMLPEFSAqlvwEKFLSS-QSPRVNVFM 297
Cdd:cd05911 167 SHRNLiaNLSQVQTFLYGNDGSNDVILGFLPLYHIYGL-FTTLASLLNGATVIIMPKFDS----ELFLDLiEKYKITFLY 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 298 AVPTIYSKLMDY--HDRHfgqphvrDFvravceEKIRLMVSGSAALPLPVLEKWKGATGHT-LLERYGMTEIGMALSNPL 374
Cdd:cd05911 242 LVPPIAAALAKSplLDKY-------DL------SSLRVILSGGAPLSKELQELLAKRFPNAtIKQGYGMTETGGILTVNP 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 375 ATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaegnETETRVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTS 454
Cdd:cd05911 309 DGDDKPGSVGRLLPNVEAKIVDD----------------DGKDSLGPN---EPGEICVRGPQVMKGYYNNPEATKETFDE 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 455 DGWFKTGDTAVF-KDGSYWIRGRtSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSL 533
Cdd:cd05911 370 DGWLHTGDIGYFdEDGYLYIVDR-KKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPGEKL 448
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 743747175 534 SHRELKEWARGVLAPYavpSEL----LLVEEIPRNQMGKVN 570
Cdd:cd05911 449 TEKEVKDYVAKKVASY---KQLrggvVFVDEIPKSASGKIL 486
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
73-577 |
4.57e-79 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 259.51 E-value: 4.57e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLRACSDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PRK08314 37 SYRELLEEAERLAGYLQQECGVRKGD----RVLLYMQNSPQFVIAYYAILRANAVVVPVNPMNREEELAHYVTDSGARVA 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHVELLSPVVRKLGVPLLPLppTVYCGAAKDPGEEQVPER----------------DW----------------RD 200
Cdd:PRK08314 113 IVGSELAPKVAPAVGNLRLRHVIV--AQYSDYLPAEPEIAVPAWlraepplqalapggvvAWkealaaglappphtagPD 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 201 RGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLP----EF 276
Cdd:PRK08314 191 DLAVLPYTSGTTGVPKGCMHTHRTVMANAVGSVLWSNSTPESVVLAVLPLFHVTGMVHSMNAPIYAGATVVLMPrwdrEA 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 277 SAQLVwEKFlssqspRVNVFMAVPTIyskLMDYhdrhFGQPHVRDFvravCEEKIRLMVSGSAALPLPVLEKWKGATGHT 356
Cdd:PRK08314 271 AARLI-ERY------RVTHWTNIPTM---VVDF----LASPGLAER----DLSSLRYIGGGGAAMPEAVAERLKELTGLD 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 357 LLERYGMTE-IGMALSNPLATARLpGSVGTPLPGVEVRIVseNPqkdgcpyiihaegnETETRVTPGfeeTEGELLVRGP 435
Cdd:PRK08314 333 YVEGYGLTEtMAQTHSNPPDRPKL-QCLGIPTFGVDARVI--DP--------------ETLEELPPG---EVGEIVVHGP 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 436 SVFRGYWDKPEETKNAF-TSDG--WFKTGDTA-VFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIG 511
Cdd:PRK08314 393 QVFKGYWNRPEATAEAFiEIDGkrFFRTGDLGrMDEEGYFFITDRLK-RMINASGFKVWPAEVENLLYKHPAIQEACVIA 471
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 743747175 512 VPDVTWGQRVTAVVTLREGH--SLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQ 577
Cdd:PRK08314 472 TPDPRRGETVKAVVVLRPEArgKTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKILWRQLQEQ 539
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
72-574 |
8.64e-78 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 252.21 E-value: 8.64e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 72 HTYRDLYSRSlslAREICRLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSV 151
Cdd:cd05934 4 WTYAELLRES---ARIAAALAAL--GIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQL 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 152 VLAgqehvellspvvrklgvpllplpptvycgaakDPgeeqvperdwrdrgAMIIYTSGTTGRPKGVVITHHNVWATVTG 231
Cdd:cd05934 79 VVV--------------------------------DP--------------ASILYTSGTTGPPKGVVITHANLTFAGYY 112
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 232 LVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWEKFLSSqspRVNVFMAVPTIYSKLMDyhd 311
Cdd:cd05934 113 SARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVLLPRFSASRFWSDVRRY---GATVTNYLGAMLSYLLA--- 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 312 rhfgQPhvrdfvRAVCEEKIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIGMALSNPLATARLPGSVGTPLPGVE 391
Cdd:cd05934 187 ----QP------PSPDDRAHRLRAAYGAPNPPELHEEFEERFGVRLLEGYGMTETIVGVIGPRDEPRRPGSIGRPAPGYE 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 392 VRIVSENpqkdgcpyiihaeGNETETRVTpgfeeteGELLVR---GPSVFRGYWDKPEETKNAFtSDGWFKTGDTAVF-K 467
Cdd:cd05934 257 VRIVDDD-------------GQELPAGEP-------GELVIRglrGWGFFKGYYNMPEATAEAM-RNGWFHTGDLGYRdA 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 468 DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLA 547
Cdd:cd05934 316 DGFFYFVDRKK-DMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPEELFAFCEGQLA 394
|
490 500
....*....|....*....|....*..
gi 743747175 548 PYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05934 395 YFKVPRYIRFVDDLPKTPTEKVAKAQL 421
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
71-574 |
1.18e-76 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 249.70 E-value: 1.18e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 71 SHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSS 150
Cdd:cd05935 1 SLTYLELLEVVKKLASFLSNK-----GVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAK 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 151 VVLAGQEHVELlspvvrklgvpllplpptvycgaakdpgeeqvperdwrdrgAMIIYTSGTTGRPKGVVITHHNVWATVT 230
Cdd:cd05935 76 VAVVGSELDDL-----------------------------------------ALIPYTSGTTGLPKGCMHTHFSAAANAL 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 231 GLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWEKFlssQSPRVNVFMAVPTIYSKLMdyh 310
Cdd:cd05935 115 QSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMARWDRETALELI---EKYKVTFWTNIPTMLVDLL--- 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 311 drhfGQPHVRDFvravCEEKIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTE-IGMALSNPLATARLPgSVGTPLPG 389
Cdd:cd05935 189 ----ATPEFKTR----DLSSLKVLTGGGAPMPPAVAEKLLKLTGLRFVEGYGLTEtMSQTHTNPPLRPKLQ-CLGIP*FG 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 390 VEVRIVSEnpqkdgcpyiihaegnETETRVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDG---WFKTGDTAVF 466
Cdd:cd05935 260 VDARVIDI----------------ETGRELPPN---EVGEIVVRGPQIFKGYWNRPEETEESFIEIKgrrFFRTGDLGYM 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 467 -KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGH--SLSHRELKEWAR 543
Cdd:cd05935 321 dEEGYFFFVDRVK-RMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYrgKVTEEDIIEWAR 399
|
490 500 510
....*....|....*....|....*....|.
gi 743747175 544 GVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05935 400 EQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
46-572 |
4.05e-76 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 253.10 E-value: 4.05e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 46 VFTRALAFGDRVALADQHGshipaG---SHTYRDLYSRSLSLAReicRLRACsdGDLREERVSFLCSNDVSYVVAQWASW 122
Cdd:COG1022 17 LRRRAARFPDRVALREKED-----GiwqSLTWAEFAERVRALAA---GLLAL--GVKPGDRVAILSDNRPEWVIADLAIL 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 123 MSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAG-QEHVELLSPVVRKL----------GVPLLPLPPTVYCGAAKDPGEE 191
Cdd:COG1022 87 AAGAVTVPIYPTSSAEEVAYILNDSGAKVLFVEdQEQLDKLLEVRDELpslrhivvldPRGLRDDPRLLSLDELLALGRE 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 192 QVPERDWRDRG--------AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCp 263
Cdd:COG1022 167 VADPAELEARRaavkpddlATIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLPLAHVFERTVSYYA- 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 264 LWVGATCV----------MLPEFSAQL------VWEKFLSS------QSPRV-----NVFMAVPTIYSKLMDYHDRH--- 313
Cdd:COG1022 246 LAAGATVAfaespdtlaeDLREVKPTFmlavprVWEKVYAGiqakaeEAGGLkrklfRWALAVGRRYARARLAGKSPsll 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 314 ------FGQPHVRDFVRAVCEEKIRLMVSGSAALPlPVLEKWKGATGHTLLERYGMTEI-GMALSNPLATARlPGSVGTP 386
Cdd:COG1022 326 lrlkhaLADKLVFSKLREALGGRLRFAVSGGAALG-PELARFFRALGIPVLEGYGLTETsPVITVNRPGDNR-IGTVGPP 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 387 LPGVEVRIvsenpqkdgcpyiihAEgnetetrvtpgfeetEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF 466
Cdd:COG1022 404 LPGVEVKI---------------AE---------------DGEILVRGPNVMKGYYKNPEATAEAFDADGWLHTGDIGEL 453
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 467 -KDGSYWIRGRTSvDIIKT-GGYKVSALEVERLLLGHPSIADVAVIGvpDvtwgQR--VTAVVTLREGhslshrELKEWA 542
Cdd:COG1022 454 dEDGFLRITGRKK-DLIVTsGGKNVAPQPIENALKASPLIEQAVVVG--D----GRpfLAALIVPDFE------ALGEWA 520
|
570 580 590
....*....|....*....|....*....|....*.
gi 743747175 543 --RGVlaPYAVPSELL----LVEEIpRNQMGKVNKR 572
Cdd:COG1022 521 eeNGL--PYTSYAELAqdpeVRALI-QEEVDRANAG 553
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
102-578 |
1.24e-73 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 244.46 E-value: 1.24e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 102 ERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPLLPLPPTVY 181
Cdd:PRK08316 62 DRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEELAYILDHSGARAFLVDPALAPTAEAALALLPVDTLILSLVLG 141
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 182 CGAAKD----------PGEEQVPERDWRDRG-AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPL 250
Cdd:PRK08316 142 GREAPGgwldfadwaeAGSVAEPDVELADDDlAQILYTSGTESLPKGAMLTHRALIAEYVSCIVAGDMSADDIPLHALPL 221
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 251 HHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWEKFlssQSPRVNVFMAVPTIYSKLMdyhdRH--FGQPHVRDFVRAVce 328
Cdd:PRK08316 222 YHCAQLDVFLGPYLYVGATNVILDAPDPELILRTI---EAERITSFFAPPTVWISLL----RHpdFDTRDLSSLRKGY-- 292
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 329 ekirlmvSGSAALPLPVLEKwkgatghtLLER---------YGMTEIGmalsnPLAT-------ARLPGSVGTPLPGVEV 392
Cdd:PRK08316 293 -------YGASIMPVEVLKE--------LRERlpglrfyncYGQTEIA-----PLATvlgpeehLRRPGSAGRPVLNVET 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 393 RIVSEnpqkdgcpyiihaEGNEtetrVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTAVF-KDGSY 471
Cdd:PRK08316 353 RVVDD-------------DGND----VAPG---EVGEIVHRSPQLMLGYWDDPEKTAEAF-RGGWFHSGDLGVMdEEGYI 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 472 WIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAV 551
Cdd:PRK08316 412 TVVDRKK-DMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATVTEDELIAHCRARLAGFKV 490
|
490 500
....*....|....*....|....*..
gi 743747175 552 PSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK08316 491 PKRVIFVDELPRNPSGKILKRELRERY 517
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
46-574 |
5.36e-71 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 236.75 E-value: 5.36e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 46 VFTRALAFGDRVALADQHGSHipagSHTYRDLYSRSLSLAREICRlRACSDGDLreerVSFLCSNDVSYVVAQWASwMSG 125
Cdd:cd05904 11 SFLFASAHPSRPALIDAATGR----ALTYAELERRVRRLAAGLAK-RGGRKGDV----VLLLSPNSIEFPVAFLAV-LSL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 126 G----TAVPLYRkhpQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVP--LLPLPPTVYCGAAKDPGEEQVPERDWR 199
Cdd:cd05904 81 GavvtTANPLST---PAEIAKQVKDSGAKLAFTTAELAEKLASLALPVVLLdsAEFDSLSFSDLLFEADEAEPPVVVIKQ 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 200 DRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWT--KDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFS 277
Cdd:cd05904 158 DDVAALLYSSGTTGRSKGVMLTHRNLIAMVAQFVAGEGSNsdSEDVFLCVLPMFHIYGLSSFALGLLRLGATVVVMPRFD 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 278 AqlvwEKFLSS-QSPRVNVFMAVPTIYSKLMDyhdrhfgQPHVRDFVRAvceeKIRLMVSGSAALPLPVLEKWKGATGHT 356
Cdd:cd05904 238 L----EELLAAiERYKVTHLPVVPPIVLALVK-------SPIVDKYDLS----SLRQIMSGAAPLGKELIEAFRAKFPNV 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 357 -LLERYGMTE---IGMALSNPLATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaegnETETRVTPGfeeTEGELLV 432
Cdd:cd05904 303 dLGQGYGMTEstgVVAMCFAPEKDRAKYGSVGRLVPNVEAKIVDP----------------ETGESLPPN---QTGELWI 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 433 RGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIG 511
Cdd:cd05904 364 RGPSIMKGYLNNPEATAATIDKEGWLHTGDLCYIdEDGYLFIVDRLK-ELIKYKGFQVAPAELEALLLSHPEILDAAVIP 442
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 743747175 512 VPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05904 443 YPDEEAGEVPMAFVVRKPGSSLTEDEIMDFVAKQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
73-571 |
3.08e-70 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 233.04 E-value: 3.08e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLRACSDgdlreERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:cd05903 3 TYSELDTRADRLAAGLAALGVGPG-----DVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVF 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHvellspvvRKLGVpllplpptvycgaAKDPGEEqvperdwrdrgAMIIYTSGTTGRPKGVVITHHNVWATVTGL 232
Cdd:cd05903 78 VVPERF--------RQFDP-------------AAMPDAV-----------ALLLFTSGTTGEPKGVMHSHNTLSASIRQY 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 233 VHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWEkflSSQSPRVNVFMAVPTIYSKLMDYHDr 312
Cdd:cd05903 126 AERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIWDPDKALA---LMREHGVTFMMGATPFLTDLLNAVE- 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 313 hFGQPHVRDFvravceekiRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIGMALSN---PLATARLpGSVGTPLPG 389
Cdd:cd05903 202 -EAGEPLSRL---------RTFVCGGATVPRSLARRAAELLGAKVCSAYGSTECPGAVTSitpAPEDRRL-YTDGRPLPG 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 390 VEVRIVSENPQKdgcpyiihaegnetetrVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTAVFKDG 469
Cdd:cd05903 271 VEIKVVDDTGAT-----------------LAPG---VEGELLSRGPSVFLGYLDRPDLTADAA-PEGWFRTGDLARLDED 329
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 470 SYW-IRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGV-LA 547
Cdd:cd05903 330 GYLrITGRSK-DIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGALLTFDELVAYLDRQgVA 408
|
490 500
....*....|....*....|....
gi 743747175 548 PYAVPSELLLVEEIPRNQMGKVNK 571
Cdd:cd05903 409 KQYWPERLVHVDDLPRTPSGKVQK 432
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
73-572 |
3.22e-69 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 230.56 E-value: 3.22e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAReicRLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:cd05907 7 TWAEFAEEVRALAK---GLIAL--GVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKAL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGqehvellspvvrklgvpllplpptvycgaakDPgeeqvperdwrDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGL 232
Cdd:cd05907 82 FVE-------------------------------DP-----------DDLATIIYTSGTTGRPKGVMLSHRNILSNALAL 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 233 VHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEfsaqlvwEKFLSSQSPRV--NVFMAVPTIYSKLMDYH 310
Cdd:cd05907 120 AERLPATEGDRHLSFLPLAHVFERRAGLYVPLLAGARIYFASS-------AETLLDDLSEVrpTVFLAVPRVWEKVYAAI 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 311 DRHFGQPHVRDFVRAVCEEKIRLMVSGSAALPLPVLEKWKGAtGHTLLERYGMTEIGMALS-NPLATARlPGSVGTPLPG 389
Cdd:cd05907 193 KVKAVPGLKRKLFDLAVGGRLRFAASGGAPLPAELLHFFRAL-GIPVYEGYGLTETSAVVTlNPPGDNR-IGTVGKPLPG 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 390 VEVRIVSEnpqkdgcpyiihaegnetetrvtpgfeeteGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFK-D 468
Cdd:cd05907 271 VEVRIADD------------------------------GEILVRGPNVMLGYYKNPEATAEALDADGWLHTGDLGEIDeD 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 469 GSYWIRGRtSVDIIKT-GGYKVSALEVERLLLGHPSIADVAVIGvpDvtwgQR--VTAVVTLREGhslshrELKEWARGV 545
Cdd:cd05907 321 GFLHITGR-KKDLIITsGGKNISPEPIENALKASPLISQAVVIG--D----GRpfLVALIVPDPE------ALEAWAEEH 387
|
490 500 510
....*....|....*....|....*....|.
gi 743747175 546 LAPYAVPSELL----LVEEIpRNQMGKVNKR 572
Cdd:cd05907 388 GIAYTDVAELAanpaVRAEI-EAAVEAANAR 417
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
73-577 |
1.74e-68 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 231.85 E-value: 1.74e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAV---PLYRKHpqaELEYFIQDSRS 149
Cdd:PRK06710 51 TFSVFHDKVKRFANYLQKL-----GVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVqtnPLYTER---ELEYQLHDSGA 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 150 SVVLAGQ---------------EHV-------------ELLSPVVRKLG---VPLLPLPPTVYCGAA----KDPGEEQV- 193
Cdd:PRK06710 123 KVILCLDlvfprvtnvqsatkiEHVivtriadflpfpkNLLYPFVQKKQsnlVVKVSESETIHLWNSvekeVNTGVEVPc 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 194 -PERDWrdrgAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHkWAWT---KDDVILHVLPLHHVHGVVNKLLCPLWVGAT 269
Cdd:PRK06710 203 dPENDL----ALLQYTGGTTGFPKGVMLTHKNLVSNTLMGVQ-WLYNckeGEEVVLGVLPFFHVYGMTAVMNLSIMQGYK 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 270 CVMLPEFSAQLVWEKFlssQSPRVNVFMAVPTIYSKLMDyhdrhfgQPHVRDFVRAvceeKIRLMVSGSAALPLPVLEKW 349
Cdd:PRK06710 278 MVLIPKFDMKMVFEAI---KKHKVTLFPGAPTIYIALLN-------SPLLKEYDIS----SIRACISGSAPLPVEVQEKF 343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 350 KGATGHTLLERYGMTEIG-MALSNPLATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaegnETETRVTPGfeeTEG 428
Cdd:PRK06710 344 ETVTGGKLVEGYGLTESSpVTHSNFLWEKRVPGSIGVPWPDTEAMIMSL----------------ETGEALPPG---EIG 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 429 ELLVRGPSVFRGYWDKPEETKnAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADV 507
Cdd:PRK06710 405 EIVVKGPQIMKGYWNKPEETA-AVLQDGWLHTGDVGYMdEDGFFYVKDRKK-DMIVASGFNVYPREVEEVLYEHEKVQEV 482
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 508 AVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQ 577
Cdd:PRK06710 483 VTIGVPDPYRGETVKAFVVLKEGTECSEEELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLIEE 552
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
49-578 |
3.67e-68 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 228.69 E-value: 3.67e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALADQHGSHipagshTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:PRK03640 11 RAFLTPDRTAIEFEEKKV------TFMELHEAVVSVAGKLAAL-----GVKKGDRVALLMKNGMEMILVIHALQQLGAVA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLgvpllplpptvYCGAAKDPGEEQVPERDWR-DRGAMIIY 207
Cdd:PRK03640 80 VLLNTRLSREELLWQLDDAEVKCLITDDDFEAKLIPGISVK-----------FAELMNGPKEEAEIQEEFDlDEVATIMY 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 208 TSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGvVNKLLCPLWVGATCVMLPEFSAQLVWEkflS 287
Cdd:PRK03640 149 TSGTTGKPKGVIQTYGNHWWSAVGSALNLGLTEDDCWLAAVPIFHISG-LSILMRSVIYGMRVVLVEKFDAEKINK---L 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 288 SQSPRVNVFMAVPTIYSKLM-DYHDRHfgqphvrdfvravCEEKIRLMVSGSAALPLPVLEKWKgATGHTLLERYGMTEI 366
Cdd:PRK03640 225 LQTGGVTIISVVSTMLQRLLeRLGEGT-------------YPSSFRCMLLGGGPAPKPLLEQCK-EKGIPVYQSYGMTET 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 367 G---MALSNPLATARLpGSVGTPLPGVEVRIvsenpQKDGcpyiihAEGNETEtrvtpgfeetEGELLVRGPSVFRGYWD 443
Cdd:PRK03640 291 AsqiVTLSPEDALTKL-GSAGKPLFPCELKI-----EKDG------VVVPPFE----------EGEIVVKGPNVTKGYLN 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 444 KPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVT 522
Cdd:PRK03640 349 REDATRETF-QDGWFKTGDIGYLdEEGFLYVLDRRS-DLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPV 426
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 523 AVVTLreGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK03640 427 AFVVK--SGEVTEEELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQLV 480
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
73-574 |
9.88e-67 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 223.37 E-value: 9.88e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:cd05972 2 SFRELKRESAKAANVLAKL-----GLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAI 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEhvellspvvrklgvpllplpptvycgaakDPgeeqvperdwrdrgAMIIYTSGTTGRPKGVVITHHNVWATVTGL 232
Cdd:cd05972 77 VTDAE-----------------------------DP--------------ALIYFTSGTTGLPKGVLHTHSYPLGHIPTA 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 233 VHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVM--LPEFSAQLVWEKFlssQSPRVNVFMAVPTIYSKLMDYh 310
Cdd:cd05972 114 AYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVyeGPRFDAERILELL---ERYGVTSFCGPPTAYRMLIKQ- 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 311 drhfgQPHVRDFVRavceekIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIGMALSNPLATARLPGSVGTPLPGV 390
Cdd:cd05972 190 -----DLSSYKFSH------LRLVVSAGEPLNPEVIEWWRAATGLPIRDGYGQTETGLTVGNFPDMPVKPGSMGRPTPGY 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 391 EVRIvsenpqkdgcpyiIHAEGNEtetrVTPGfeeTEGELLVR--GPSVFRGYWDKPEETKNAFtSDGWFKTGDTAVF-K 467
Cdd:cd05972 259 DVAI-------------IDDDGRE----LPPG---EEGDIAIKlpPPGLFLGYVGDPEKTEASI-RGDYYLTGDRAYRdE 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 468 DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSH---RELKEWARG 544
Cdd:cd05972 318 DGYFWFVGRAD-DIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEelaEELQGHVKK 396
|
490 500 510
....*....|....*....|....*....|
gi 743747175 545 VLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05972 397 VLAPYKYPREIEFVEELPKTISGKIRRVEL 426
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
49-578 |
1.26e-66 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 226.18 E-value: 1.26e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALADqhgshiPAGSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGgtA 128
Cdd:COG1021 34 RAERHPDRIAVVD------GERRLSYAELDRRADRLAAGLLAL-----GLRPGDRVVVQLPNVAEFVIVFFALFRAG--A 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLY--RKHPQAELEYFIQDSRSSVVLAGQEH-----VELLS------PVVRKLGVPLLPLPPTVYCGAAKDPGEEQVPE 195
Cdd:COG1021 101 IPVFalPAHRRAEISHFAEQSEAVAYIIPDRHrgfdyRALARelqaevPSLRHVLVVGDAGEFTSLDALLAAPADLSEPR 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 196 RDWRDRGAMIIyTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHH--------VHGVvnkllcpLWVG 267
Cdd:COG1021 181 PDPDDVAFFQL-SGGTTGLPKLIPRTHDDYLYSVRASAEICGLDADTVYLAALPAAHnfplsspgVLGV-------LYAG 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 268 ATCVMLPEFSAQ----LVwEKflssqsPRVNVFMAVPTIYSKLMDYHDRHFGQPhvrdfvravceEKIRLMVSGSAALPL 343
Cdd:COG1021 253 GTVVLAPDPSPDtafpLI-ER------ERVTVTALVPPLALLWLDAAERSRYDL-----------SSLRVLQVGGAKLSP 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 344 PVLEKWKGATGHTLLERYGMTEiGM----ALSNPLATARlpGSVGTPL-PGVEVRIVSEnpqkdgcpyiihaEGNEtetr 418
Cdd:COG1021 315 ELARRVRPALGCTLQQVFGMAE-GLvnytRLDDPEEVIL--TTQGRPIsPDDEVRIVDE-------------DGNP---- 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 419 VTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRtSVDIIKTGGYKVSALEVERL 497
Cdd:COG1021 375 VPPG---EVGELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVRRtPDGYLVVEGR-AKDQINRGGEKIAAEEVENL 450
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 498 LLGHPSIADVAVIGVPDVTWGQRVTAVVTLReGHSLSHRELKEW--ARGVlAPYAVPSELLLVEEIPRNQMGKVNKRDLV 575
Cdd:COG1021 451 LLAHPAVHDAAVVAMPDEYLGERSCAFVVPR-GEPLTLAELRRFlrERGL-AAFKLPDRLEFVDALPLTAVGKIDKKALR 528
|
...
gi 743747175 576 QQL 578
Cdd:COG1021 529 AAL 531
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
72-574 |
1.82e-66 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 225.20 E-value: 1.82e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 72 HTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGG---TAVPlyRKHPQaELEYFIQDSR 148
Cdd:cd12119 26 YTYAEVAERARRLANALRRL-----GVKPGDRVATLAWNTHRHLELYYAVPGMGAvlhTINP--RLFPE-QIAYIINHAE 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 149 SSVVLAGQEHVELLSPVVRKLGVPLLPLPPTVYCGA---------------AKDPGEEQVPERDWRDrGAMIIYTSGTTG 213
Cdd:cd12119 98 DRVVFVDRDFLPLLEAIAPRLPTVEHVVVMTDDAAMpepagvgvlayeellAAESPEYDWPDFDENT-AAAICYTSGTTG 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 214 RPKGVVITHHNVW--------ATVTGLVHKwawtkdDVILHVLPLHHVH--GVvnkllcP---LWVGATCV-----MLPE 275
Cdd:cd12119 177 NPKGVVYSHRSLVlhamaallTDGLGLSES------DVVLPVVPMFHVNawGL------PyaaAMVGAKLVlpgpyLDPA 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 276 FSAQLVwekflssQSPRVNVFMAVPTIYSKLMDYHDRHfgqPHVRDFVRAVceekirlmVSGSAALPLPVLEKWKgATGH 355
Cdd:cd12119 245 SLAELI-------EREGVTFAAGVPTVWQGLLDHLEAN---GRDLSSLRRV--------VIGGSAVPRSLIEAFE-ERGV 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 356 TLLERYGMTEIGmalsnPLAT-ARLPGSV---------------GTPLPGVEVRIVSEnpqkdgcpyiihaegnetETRV 419
Cdd:cd12119 306 RVIHAWGMTETS-----PLGTvARPPSEHsnlsedeqlalrakqGRPVPGVELRIVDD------------------DGRE 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 420 TPGFEETEGELLVRGPSVFRGYWDKPEETKnAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLL 498
Cdd:cd12119 363 LPWDGKAVGELQVRGPWVTKSYYKNDEESE-ALTEDGWLRTGDVATIdEDGYLTITDRSK-DVIKSGGEWISSVELENAI 440
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 499 LGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd12119 441 MAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGATVTAEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKAL 516
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
49-578 |
4.71e-66 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 223.58 E-value: 4.71e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALADQHGSHipagshTYRDLYSRSLSLAREICRLRACSDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:PRK06839 11 RAYLHPDRIAIITEEEEM------TYKQLHEYVSKVAAYLIYELNVKKGE----RIAILSQNSLEYIVLLFAIAKVECIA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPLLPLPptvycgaaKDPGEEQVPERD-----WRDRGA 203
Cdd:PRK06839 81 VPLNIRLTENELIFQLKDSGTTVLFVEKTFQNMALSMQKVSYVQRVISI--------TSLKEIEDRKIDnfvekNESASF 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 204 MIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVvNKLLCPLWVGATCVML-----PEFSA 278
Cdd:PRK06839 153 IICYTSGTTGKPKGAVLTQENMFWNALNNTFAIDLTMHDRSIVLLPLFHIGGI-GLFAFPTLFAGGVIIVprkfePTKAL 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 QLVwekflssQSPRVNVFMAVPTIYSKLMDYHDrhFGQPHVrdfvravceEKIRLMVSGSAALPLPVLEKWKgATGHTLL 358
Cdd:PRK06839 232 SMI-------EKHKVTVVMGVPTIHQALINCSK--FETTNL---------QSVRWFYNGGAPCPEELMREFI-DRGFLFG 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 359 ERYGMTE----IGMALSNPlaTARLPGSVGTPLPGVEVRIVSENPQKdgcpyiihaegnetetrVTPGfeeTEGELLVRG 434
Cdd:PRK06839 293 QGFGMTEtsptVFMLSEED--ARRKVGSIGKPVLFCDYELIDENKNK-----------------VEVG---EVGELLIRG 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 435 PSVFRGYWDKPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVP 513
Cdd:PRK06839 351 PNVMKEYWNRPDATEETI-QDGWLCTGDLARVdEDGFVYIVGRKK-EMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQ 428
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 743747175 514 DVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK06839 429 HVKWGEIPIAFIVKKSSSVLIEKDVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQLVNQL 493
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
71-576 |
5.73e-66 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 221.07 E-value: 5.73e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 71 SHTYRDLYSRSLSLAReicRLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSS 150
Cdd:cd05912 1 SYTFAELFEEVSRLAE---HLAAL--GVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDVK 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 151 VvlagqehvellspvvrklgvpllplpptvycgaakdpgeeqvperdwrDRGAMIIYTSGTTGRPKGVVITHHNVWATVT 230
Cdd:cd05912 76 L------------------------------------------------DDIATIMYTSGTTGKPKGVQQTFGNHWWSAI 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 231 GLVHKWAWTKDDVILHVLPLHHVHGvVNKLLCPLWVGATCVMLPEFSAQLVWEkflSSQSPRVNVFMAVPTIYSKLMDYH 310
Cdd:cd05912 108 GSALNLGLTEDDNWLCALPLFHISG-LSILMRSVIYGMTVYLVDKFDAEQVLH---LINSGKVTIISVVPTMLQRLLEIL 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 311 DRHfgqphvrdfvravCEEKIRLMVSGSAALPLPVLEKWKgATGHTLLERYGMTEIG--MALSNPLATARLPGSVGTPLP 388
Cdd:cd05912 184 GEG-------------YPNNLRCILLGGGPAPKPLLEQCK-EKGIPVYQSYGMTETCsqIVTLSPEDALNKIGSAGKPLF 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 389 GVEVRIVSENpqkdgcpyiihaegnetetrvtpGFEETEGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTAVF-K 467
Cdd:cd05912 250 PVELKIEDDG-----------------------QPPYEVGEILLKGPNVTKGYLNRPDATEESF-ENGWFKTGDIGYLdE 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 468 DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREghSLSHRELKEWARGVLA 547
Cdd:cd05912 306 EGFLYVLDRRS-DLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSER--PISEEELIAYCSEKLA 382
|
490 500
....*....|....*....|....*....
gi 743747175 548 PYAVPSELLLVEEIPRNQMGKVNKRDLVQ 576
Cdd:cd05912 383 KYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
204-571 |
2.38e-64 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 214.06 E-value: 2.38e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 204 MIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVvNKLLCPLWVGATCVMLPEFSAQLVWE 283
Cdd:cd17637 4 VIIHTAAVAGRPRGAVLSHGNLIAANLQLIHAMGLTEADVYLNMLPLFHIAGL-NLALATFHAGGANVVMEKFDPAEALE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 KflsSQSPRVNVFMAVPTIYSKLMDYHDRHFGQPhvrDFVRAVceekirlmvsgsAALPLP-VLEKWKGATGHTLLERYG 362
Cdd:cd17637 83 L---IEEEKVTLMGSFPPILSNLLDAAEKSGVDL---SSLRHV------------LGLDAPeTIQRFEETTGATFWSLYG 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 363 MTEIGMALSNPLATARlPGSVGTPLPGVEVRIVSENpqkdgcpyiihaeGNEtetrVTPGfeeTEGELLVRGPSVFRGYW 442
Cdd:cd17637 145 QTETSGLVTLSPYRER-PGSAGRPGPLVRVRIVDDN-------------DRP----VPAG---ETGEIVVRGPLVFQGYW 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 443 DKPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSV-DIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQR 520
Cdd:cd17637 204 NLPELTAYTF-RNGWHHTGDLGRFdEDGYLWYAGRKPEkELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEG 282
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|.
gi 743747175 521 VTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNK 571
Cdd:cd17637 283 IKAVCVLKPGATLTADELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
31-574 |
1.06e-63 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 217.93 E-value: 1.06e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 31 PLHTGPAARGNRSAPVFTRALA-FGDRVALADqhgshiPAGSHTYRDLysrslslAREICR----LRACsdGDLREERVS 105
Cdd:PRK06188 2 ATMADLLHSGATYGHLLVSALKrYPDRPALVL------GDTRLTYGQL-------ADRISRyiqaFEAL--GLGTGDAVA 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 106 FLCSNDVSYVVAQWASWMSGGTAVPLyrkHPQAELE---YFIQDSRSSVVLA--------GQEHVELLSPVVRKLGVPLL 174
Cdd:PRK06188 67 LLSLNRPEVLMAIGAAQLAGLRRTAL---HPLGSLDdhaYVLEDAGISTLIVdpapfverALALLARVPSLKHVLTLGPV 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 175 PLPPTVYCGAAKDPGEEQVPERDWRDRgAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVH 254
Cdd:PRK06188 144 PDGVDLLAAAAKFGPAPLVAAALPPDI-AGLAYTGGTTGKPKGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSHAG 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 255 GVvnKLLCPLWVGATCVMLPEFSAqlvwEKFLSS-QSPRVNVFMAVPTIYSKLMDYHDrhfgqPHVRDFvravceEKIRL 333
Cdd:PRK06188 223 GA--FFLPTLLRGGTVIVLAKFDP----AEVLRAiEEQRITATFLVPTMIYALLDHPD-----LRTRDL------SSLET 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 334 MVSGSAALpLPV-LEKWKGATGHTLLERYGMTEIGMALS------NPLATARLPGSVGTPLPGVEVRIVSENpqkdgcpy 406
Cdd:PRK06188 286 VYYGASPM-SPVrLAEAIERFGPIFAQYYGQTEAPMVITylrkrdHDPDDPKRLTSCGRPTPGLRVALLDED-------- 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 407 iihaeGNEtetrVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTG 485
Cdd:PRK06188 357 -----GRE----VAQG---EVGEICVRGPLVMDGYWNRPEETAEAF-RDGWLHTGDVAREdEDGFYYIVDRKK-DMIVTG 422
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 486 GYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQ 565
Cdd:PRK06188 423 GFNVFPREVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAAVDAAELQAHVKERKGSVHAPKQVDFVDSLPLTA 502
|
....*....
gi 743747175 566 MGKVNKRDL 574
Cdd:PRK06188 503 LGKPDKKAL 511
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
205-574 |
1.21e-63 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 212.91 E-value: 1.21e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITHHNVW--ATVTGlvHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVML-PEFSAQLV 281
Cdd:cd05917 7 IQFTSGTTGSPKGATLTHHNIVnnGYFIG--ERLGLTEQDRLCIPVPLFHCFGSVLGVLACLTHGATMVFPsPSFDPLAV 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 282 wekFLSSQSPRVNVFMAVPTIYSKLMDYHDrhFGQphvRDFVRavceekIRLMVSGSAALPLPVLEKwkgatghtLLERY 361
Cdd:cd05917 85 ---LEAIEKEKCTALHGVPTMFIAELEHPD--FDK---FDLSS------LRTGIMAGAPCPPELMKR--------VIEVM 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 362 GMTEI----GMALSNPLATARLPG--------SVGTPLPGVEVRIVSEnpqkdgcpyiihaegnetETRVTPGFEETeGE 429
Cdd:cd05917 143 NMKDVtiayGMTETSPVSTQTRTDdsiekrvnTVGRIMPHTEAKIVDP------------------EGGIVPPVGVP-GE 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 430 LLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVA 508
Cdd:cd05917 204 LCIRGYSVMKGYWNDPEKTAEAIDGDGWLHTGDLAVMdEDGYCRIVGRIK-DMIIRGGENIYPREIEEFLHTHPKVSDVQ 282
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 509 VIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05917 283 VVGVPDERYGEEVCAWIRLKEGAELTEEDIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKL 348
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
55-574 |
2.01e-63 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 215.08 E-value: 2.01e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADQHGSHipagshTYRDLYSRSLSLAReicRLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRK 134
Cdd:cd05930 2 DAVAVVDGDQSL------TYAELDARANRLAR---YLRER--GVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPS 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 135 HPQAELEYFIQDSRSSVVLAGQEHVellspvvrklgvpllplpptvycgaakdpgeeqvperdwrdrgAMIIYTSGTTGR 214
Cdd:cd05930 71 YPAERLAYILEDSGAKLVLTDPDDL-------------------------------------------AYVIYTSGSTGK 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 215 PKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNkLLCPLWVGATCVMLPE---FSAQLVWEKFlssQSP 291
Cdd:cd05930 108 PKGVMVEHRGLVNLLLWMQEAYPLTPGDRVLQFTSFSFDVSVWE-IFGALLAGATLVVLPEevrKDPEALADLL---AEE 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 292 RVNVFMAVPTIYSKLMDYhdrhfgqPHVRDFvravceEKIRLMVSGSAALPLPVLEKWKGA-TGHTLLERYGMTEI-GMA 369
Cdd:cd05930 184 GITVLHLTPSLLRLLLQE-------LELAAL------PSLRLVLVGGEALPPDLVRRWRELlPGARLVNLYGPTEAtVDA 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 370 LSNPLATARLPGS---VGTPLPGVEVRIVSEN--PQKDGCPyiihaegnetetrvtpgfeeteGELLVRGPSVFRGYWDK 444
Cdd:cd05930 251 TYYRVPPDDEEDGrvpIGRPIPNTRVYVLDENlrPVPPGVP----------------------GELYIGGAGLARGYLNR 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 445 PEETKNAFTSDGWF------KTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTW 517
Cdd:cd05930 309 PELTAERFVPNPFGpgermyRTGDLVRWLpDGNLEFLGRID-DQVKIRGYRIELGEIEAALLAHPGVREAAVVAREDGDG 387
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 743747175 518 GQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05930 388 EKRLVAYVVPDEGGELDEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
68-574 |
1.03e-62 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 213.09 E-value: 1.03e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 68 PAGSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDS 147
Cdd:cd05919 7 ADRSVTYGQLHDGANRLGSALRNL-----GVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDC 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 148 RSSVVLAGQEHVellspvvrklgvpllplpptvycgaakdpgeeqvperdwrdrgAMIIYTSGTTGRPKGVVITHHNVWA 227
Cdd:cd05919 82 EARLVVTSADDI-------------------------------------------AYLLYSSGTTGPPKGVMHAHRDPLL 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 228 TVTGLVHKW-AWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEF-SAQLVWEKfLSSQSPRVnvFMAVPTIYSK 305
Cdd:cd05919 119 FADAMAREAlGLTPGDRVFSSAKMFFGYGLGNSLWFPLAVGASAVLNPGWpTAERVLAT-LARFRPTV--LYGVPTFYAN 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 306 LMDYHDrhFGQPHVRDfvravceekIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIG-MALSNPLATARlPGSVG 384
Cdd:cd05919 196 LLDSCA--GSPDALRS---------LRLCVSAGEALPRGLGERWMEHFGGPILDGIGATEVGhIFLSNRPGAWR-LGSTG 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 385 TPLPGVEVRIVSEnpqkdgcpyiihaEGNEtetrVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTA 464
Cdd:cd05919 264 RPVPGYEIRLVDE-------------EGHT----IPPG---EEGDLLVRGPSAAVGYWNNPEKSRATF-NGGWYRTGDKF 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 465 VF-KDGSYWIRGRtSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSH---RELKE 540
Cdd:cd05919 323 CRdADGWYTHAGR-ADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQEslaRDIHR 401
|
490 500 510
....*....|....*....|....*....|....
gi 743747175 541 WARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05919 402 HLLERLSAHKVPRRIAFVDELPRTATGKLQRFKL 435
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
68-574 |
1.31e-62 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 212.72 E-value: 1.31e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 68 PAGSHTYRDLysrsLSLAREICRLRACSDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDS 147
Cdd:cd05958 7 PEREWTYRDL----LALANRIANVLVGELGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDKA 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 148 RSSVVL-AGQEHVellspvvrklgvpllplpptvycgaakdpgeeqvperdwRDRGAMIIYTSGTTGRPKGVVITHHNVW 226
Cdd:cd05958 83 RITVALcAHALTA---------------------------------------SDDICILAFTSGTTGAPKATMHFHRDPL 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 227 ATVTGL-VHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVwekFLSSQSPRVNVFMAVPTIYSK 305
Cdd:cd05958 124 ASADRYaVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEEATPDLL---LSAIARYKPTVLFTAPTAYRA 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 306 LMDYHDrhFGQPHVrdfvravceEKIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTE-IGMALSNPLATARlPGSVG 384
Cdd:cd05958 201 MLAHPD--AAGPDL---------SSLRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTEmFHIFISARPGDAR-PGATG 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 385 TPLPGVEVRIVSEnpqkdgcpyiihaEGNEtetrVTPGfeeTEGELLVRGPSvfrGYWDKPEETKNAFTSDGWFKTGDTA 464
Cdd:cd05958 269 KPVPGYEAKVVDD-------------EGNP----VPDG---TIGRLAVRGPT---GCRYLADKRQRTYVQGGWNITGDTY 325
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 465 VFK-DGSYWIRGRtSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSH---RELKE 540
Cdd:cd05958 326 SRDpDGYFRHQGR-SDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPvlaRELQD 404
|
490 500 510
....*....|....*....|....*....|....
gi 743747175 541 WARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05958 405 HAKAHIAPYKYPRAIEFVTELPRTATGKLQRFAL 438
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
55-577 |
3.97e-62 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 214.53 E-value: 3.97e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADQHGSHIPAGSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPL--- 131
Cdd:PRK13295 39 DKTAVTAVRLGTGAPRRFTYRELAALVDRVAVGLARL-----GVGRGDVVSCQLPNWWEFTVLYLACSRIGAVLNPLmpi 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 132 YRKHpqaELEYFIQDSRSSVVLAGQE-----HVELLSPVVRKLgvpLLPLPPTVYCGAAKDPGEEQVPERDW-------- 198
Cdd:PRK13295 114 FRER---ELSFMLKHAESKVLVVPKTfrgfdHAAMARRLRPEL---PALRHVVVVGGDGADSFEALLITPAWeqepdapa 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 199 ---RDRG-----AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATC 270
Cdd:PRK13295 188 ilaRLRPgpddvTQLIYTSGTTGEPKGVMHTANTLMANIVPYAERLGLGADDVILMASPMAHQTGFMYGLMMPVMLGATA 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 271 VMlpefsaQLVWEKFLSS---QSPRVNVFMAvptiysklmdyhdrhfGQPHVRDFVRAVCEE-----KIRLMVSGSAALP 342
Cdd:PRK13295 268 VL------QDIWDPARAAeliRTEGVTFTMA----------------STPFLTDLTRAVKESgrpvsSLRTFLCAGAPIP 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 343 LPVLEKWKGATGHTLLERYGMTEIGMALSNPLATA--RLPGSVGTPLPGVEVRIVSenpqkdgcpyiihAEGNEtetrVT 420
Cdd:PRK13295 326 GALVERARAALGAKIVSAWGMTENGAVTLTKLDDPdeRASTTDGCPLPGVEVRVVD-------------ADGAP----LP 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 421 PGfeeTEGELLVRGPSVFRGYWDKPEetKNAFTSDGWFKTGDTA-VFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLL 499
Cdd:PRK13295 389 AG---QIGRLQVRGCSNFGGYLKRPQ--LNGTDADGWFDTGDLArIDADGYIRISGRSK-DVIIRGGENIPVVEIEALLY 462
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 500 GHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEW--ARGVLAPYaVPSELLLVEEIPRNQMGKVNK---RDL 574
Cdd:PRK13295 463 RHPAIAQVAIVAYPDERLGERACAFVVPRPGQSLDFEEMVEFlkAQKVAKQY-IPERLVVRDALPRTPSGKIQKfrlREM 541
|
...
gi 743747175 575 VQQ 577
Cdd:PRK13295 542 LRG 544
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
73-582 |
3.36e-61 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 210.43 E-value: 3.36e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLySRSLSLAREICRLRACSDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PRK09088 24 TYAEL-DALVGRLAAVLRRRGCVDGE----RLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEPRLL 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LaGQEHVELLSPVVRKLGVPLlplpptvycgAAKDPGEEQVPERDWRDRGAMIIYTSGTTGRPKGVVITHHNVWAT---- 228
Cdd:PRK09088 99 L-GDDAVAAGRTDVEDLAAFI----------ASADALEPADTPSIPPERVSLILFTSGTSGQPKGVMLSERNLQQTahnf 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 229 -VTGLVhkwawTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQ--LVWekfLSSQSPRVNVFMAVPTIYSK 305
Cdd:PRK09088 168 gVLGRV-----DAHSSFLCDAPMFHIIGLITSVRPVLAVGGSILVSNGFEPKrtLGR---LGDPALGITHYFCVPQMAQA 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 306 LMDyhdrhfgQPhvrDFVRAVCEeKIRLMVSGSAALPLPVLEKWKgATGHTLLERYGMTEIGMALSNPLATARLP---GS 382
Cdd:PRK09088 240 FRA-------QP---GFDAAALR-HLTALFTGGAPHAAEDILGWL-DDGIPMVDGFGMSEAGTVFGMSVDCDVIRakaGA 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 383 VGTPLPGVEVRIVSEnpQKDGCPyiihaegnetetrvtPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGD 462
Cdd:PRK09088 308 AGIPTPTVQTRVVDD--QGNDCP---------------AG---VPGELLLRGPNLSPGYWRRPQATARAFTGDGWFRTGD 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 463 TAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEW 541
Cdd:PRK09088 368 IARRDaDGFFWVVDRKK-DMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAPLDLERIRSH 446
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 743747175 542 ARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQLYPQD 582
Cdd:PRK09088 447 LSTRLAKYKVPKHLRLVDALPRTASGKLQKARLRDALAAGR 487
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
54-571 |
2.05e-60 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 208.92 E-value: 2.05e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 54 GDRVALADqhgshiPAGSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYR 133
Cdd:TIGR02262 19 GGKTAFID------DISSLSYGELEAQVRRLAAALRRL-----GVKREERVLLLMLDGVDFPIAFLGAIRAGIVPVALNT 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 134 KHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLgvpllPLPPTVYCGAAKDPGEEQV------------PERDWRDR 201
Cdd:TIGR02262 88 LLTADDYAYMLEDSRARVVFVSGALLPVIKAALGKS-----PHLEHRVVVGRPEAGEVQLaellateseqfkPAATQADD 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 202 GAMIIYTSGTTGRPKGVVITHHNVWATV-TGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEF-SAQ 279
Cdd:TIGR02262 163 PAFWLYSSGSTGMPKGVVHTHSNPYWTAeLYARNTLGIREDDVCFSAAKLFFAYGLGNALTFPMSVGATTVLMGERpTPD 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 280 LVWEKFLSSQSprvNVFMAVPTIYSKLMDYHDrhfgqphvrdfVRAVCEEKIRLMVSGSAALPLPVLEKWKGATGHTLLE 359
Cdd:TIGR02262 243 AVFDRLRRHQP---TIFYGVPTLYAAMLADPN-----------LPSEDQVRLRLCTSAGEALPAEVGQRWQARFGVDIVD 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 360 RYGMTEIG-MALSNpLATARLPGSVGTPLPGVEVRIVSENPQ--KDGCPyiihaegnetetrvtpgfeeteGELLVRGPS 436
Cdd:TIGR02262 309 GIGSTEMLhIFLSN-LPGDVRYGTSGKPVPGYRLRLVGDGGQdvADGEP----------------------GELLISGPS 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 437 VFRGYWDKPEETKNAFTSdGWFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDV 515
Cdd:TIGR02262 366 SATMYWNNRAKSRDTFQG-EWTRSGDKYVRNdDGSYTYAGRTD-DMLKVSGIYVSPFEIESALIQHPAVLEAAVVGVADE 443
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 516 TWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNK 571
Cdd:TIGR02262 444 DGLIKPKAFVVLRPGQTALETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQR 499
|
|
| menE |
TIGR01923 |
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ... |
73-574 |
2.65e-60 |
|
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]
Pssm-ID: 162605 [Multi-domain] Cd Length: 436 Bit Score: 206.53 E-value: 2.65e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAReicRLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:TIGR01923 1 TWQDLDCEAAHLAK---ALKA--QGIRSGSRVALVGQNSIEMVLLLHACLLLGAEIAMLNTRLTENERTNQLEDLDVQLL 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQ--EHVELLSPVVRKLGvpllplpptvycgaAKDPGEEQVPERDWRDRGAMIIYTSGTTGRPKGVVITHHNVWATVT 230
Cdd:TIGR01923 76 LTDSllEEKDFQADSLDRIE--------------AAGRYETSLSASFNMDQIATLMFTSGTTGKPKAVPHTFRNHYASAV 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 231 GLVHKWAWTKDDVILHVLPLHHVHGVvNKLLCPLWVGATcVMLPEFSAQLvwekFLSSQSPRVNVFMAVPTIYSKLMDYH 310
Cdd:TIGR01923 142 GSKENLGFTEDDNWLLSLPLYHISGL-SILFRWLIEGAT-LRIVDKFNQL----LEMIANERVTHISLVPTQLNRLLDEG 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 311 DRhfgqphvrdfvravcEEKIRLMVSGSAALPLPVLEKWKgATGHTLLERYGMTEIG---MALSNPLATARlpGSVGTPL 387
Cdd:TIGR01923 216 GH---------------NENLRKILLGGSAIPAPLIEEAQ-QYGLPIYLSYGMTETCsqvTTATPEMLHAR--PDVGRPL 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 388 PGVEVRIVSENpqkdgcpyiihaegnetetrvtpgfEETEGELLVRGPSVFRGYWDkPEETKNAFTSDGWFKTGDTAVFK 467
Cdd:TIGR01923 278 AGREIKIKVDN-------------------------KEGHGEIMVKGANLMKGYLY-QGELTPAFEQQGWFNTGDIGELD 331
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 468 -DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREghSLSHRELKEWARGVL 546
Cdd:TIGR01923 332 gEGFLYVLGRRD-DLIISGGENIYPEEIETVLYQHPGIQEAVVVPKPDAEWGQVPVAYIVSES--DISQAKLIAYLTEKL 408
|
490 500
....*....|....*....|....*...
gi 743747175 547 APYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:TIGR01923 409 AKYKVPIAFEKLDELPYNASGKILRNQL 436
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
50-571 |
1.31e-59 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 206.00 E-value: 1.31e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 50 ALAFGDRVALAdqHGSHipagSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAV 129
Cdd:cd12118 14 AAVYPDRTSIV--YGDR----RYTWRQTYDRCRRLASALAAL-----GISRGDTVAVLAPNTPAMYELHFGVPMAGAVLN 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 130 PLYRKHPQAELEYFIQDSRSSVVLAGQEHV--ELLspvvrklgvpllplpptvycgAAKDPGEEQVPERDWRDRGAmIIY 207
Cdd:cd12118 83 ALNTRLDAEEIAFILRHSEAKVLFVDREFEyeDLL---------------------AEGDPDFEWIPPADEWDPIA-LNY 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 208 TSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGvvnklLCPLW----VGATCVMLPEFSAQLVWE 283
Cdd:cd12118 141 TSGTTGRPKGVVYHHRGAYLNALANILEWEMKQHPVYLWTLPMFHCNG-----WCFPWtvaaVGGTNVCLRKVDAKAIYD 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 kflSSQSPRVNVFMAVPTIYSKLMDYhdrhfgqphvRDFVRAVCEEKIRLMVSGSAalPLPVLEKWKGATGHTLLERYGM 363
Cdd:cd12118 216 ---LIEKHKVTHFCGAPTVLNMLANA----------PPSDARPLPHRVHVMTAGAP--PPAAVLAKMEELGFDVTHVYGL 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 364 TEI-GMALSNPLatarLPGSVGTPLpgvEVRIVSENPQkdGCPYIIH--AEGNETET-RVTPGFEETEGELLVRGPSVFR 439
Cdd:cd12118 281 TETyGPATVCAW----KPEWDELPT---EERARLKARQ--GVRYVGLeeVDVLDPETmKPVPRDGKTIGEIVFRGNIVMK 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 440 GYWDKPEETKNAFtSDGWFKTGDTAVFK-DGSYWIRGRtSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWG 518
Cdd:cd12118 352 GYLKNPEATAEAF-RGGWFHSGDLAVIHpDGYIEIKDR-SKDIIISGGENISSVEVEGVLYKHPAVLEAAVVARPDEKWG 429
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 743747175 519 QRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSElLLVEEIPRNQMGKVNK 571
Cdd:cd12118 430 EVPCAFVELKEGAKVTEEEIIAFCREHLAGFMVPKT-VVFGELPKTSTGKIQK 481
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
55-574 |
1.95e-58 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 203.12 E-value: 1.95e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADqhgshiPAGSH--TYRDLYSRSLSLAReicrlRACSDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLY 132
Cdd:cd05923 16 DACAIAD------PARGLrlTYSELRARIEAVAA-----RLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALIN 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 133 RKHPQAELEYFIQ-DSRSSVVLA----GQEHVELLSPVVRKLGVPLLPLPPTVYCGAAKDPGEEqvPERDwrdrgAMIIY 207
Cdd:cd05923 85 PRLKAAELAELIErGEMTAAVIAvdaqVMDAIFQSGVRVLALSDLVGLGEPESAGPLIEDPPRE--PEQP-----AFVFY 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 208 TSGTTGRPKGVVITHHNV------WATVTGLVHKwawtKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLV 281
Cdd:cd05923 158 TSGTTGLPKGAVIPQRAAesrvlfMSTQAGLRHG----RHNVVLGLMPLYHVIGFFAVLVAALALDGTYVVVEEFDPADA 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 282 WEKFlssQSPRVNVFMAVPTIYSKLMdyHDRHFGQPHVrdfvravceEKIRLMVSGSAALPLPVLEKWKGATGHTLLERY 361
Cdd:cd05923 234 LKLI---EQERVTSLFATPTHLDALA--AAAEFAGLKL---------SSLRHVTFAGATMPDAVLERVNQHLPGEKVNIY 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 362 GMTEIGMALSNPLATarlPGSVGTPLPGVEVRIVSENpqkdGCPYIIHAEGNEtetrvtpgfeeteGELLVR--GPSVFR 439
Cdd:cd05923 300 GTTEAMNSLYMRDAR---TGTEMRPGFFSEVRIVRIG----GSPDEALANGEE-------------GELIVAaaADAAFT 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 440 GYWDKPEETKNAFtSDGWFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWG 518
Cdd:cd05923 360 GYLNQPEATAKKL-QDGWYRTGDVGYVDpSGDVRILGRVD-DMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWG 437
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 743747175 519 QRVTAVVTLREGhSLSHRELKEWARGV-LAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05923 438 QSVTACVVPREG-TLSADELDQFCRASeLADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
72-574 |
4.98e-58 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 200.81 E-value: 4.98e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 72 HTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSV 151
Cdd:cd05969 1 YTFAQLKVLSARFANVLKSL-----GVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKV 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 152 VLAGQEHVELLSPvvrklgvpllplpptvycgaaKDPgeeqvperdwrdrgAMIIYTSGTTGRPKGVVITHHNVWAT-VT 230
Cdd:cd05969 76 LITTEELYERTDP---------------------EDP--------------TLLHYTSGTTGTPKGVLHVHDAMIFYyFT 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 231 GlvhKWAW--TKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLP-EFSAQlVWEKFLssQSPRVNVFMAVPTIYSKLM 307
Cdd:cd05969 121 G---KYVLdlHPDDIYWCTADPGWVTGTVYGIWAPWLNGVTNVVYEgRFDAE-SWYGII--ERVKVTVWYTAPTAIRMLM 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 308 DYHD---RHFGQPHVRdFVRAVCE----EKIRLmvsGSAALPLPVLEKWkgatghtlleryGMTEIG-MALSNPLATARL 379
Cdd:cd05969 195 KEGDelaRKYDLSSLR-FIHSVGEplnpEAIRW---GMEVFGVPIHDTW------------WQTETGsIMIANYPCMPIK 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 380 PGSVGTPLPGVEVRIVSENpqkdgcpyiihaeGNETEtrvtPGfeeTEGELLVRG--PSVFRGYWDKPEETKNAFTsDGW 457
Cdd:cd05969 259 PGSMGKPLPGVKAAVVDEN-------------GNELP----PG---TKGILALKPgwPSMFRGIWNDEERYKNSFI-DGW 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 458 FKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSH- 535
Cdd:cd05969 318 YLTGDLAYRdEDGYFWFVGRAD-DIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDe 396
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 743747175 536 --RELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05969 397 lkEEIINFVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVL 437
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
185-574 |
1.27e-57 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 204.03 E-value: 1.27e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 185 AKDPGEEQVPERDWR-DRGAMIIYTSGTTGRPKGVVITH----HNVW--ATVTGLVHkwawtkDDVILHVLPLHHVHGVV 257
Cdd:PRK07529 197 ARQPGDRLFSGRPIGpDDVAAYFHTGGTTGMPKLAQHTHgnevANAWlgALLLGLGP------GDTVFCGLPLFHVNALL 270
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 258 NKLLCPLWVGATCVML-------PEFSAQLvW---EKFlssqspRVNVFMAVPTIYSKLMDyhdrhfgQPhvrdfVRAVC 327
Cdd:PRK07529 271 VTGLAPLARGAHVVLAtpqgyrgPGVIANF-WkivERY------RINFLSGVPTVYAALLQ-------VP-----VDGHD 331
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 328 EEKIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEiGMALS--NPLATARLPGSVGTPLPGVEVRIVSENPqkDGcP 405
Cdd:PRK07529 332 ISSLRYALCGAAPLPVEVFRRFEAATGVRIVEGYGLTE-ATCVSsvNPPDGERRIGSVGLRLPYQRVRVVILDD--AG-R 407
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 406 YIIHAEGNETetrvtpgfeeteGELLVRGPSVFRGYWDkPEETKNAFTSDGWFKTGDTA-VFKDGSYWIRGRTSvDIIKT 484
Cdd:PRK07529 408 YLRDCAVDEV------------GVLCIAGPNVFSGYLE-AAHNKGLWLEDGWLNTGDLGrIDADGYFWLTGRAK-DLIIR 473
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 485 GGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARG-VLAPYAVPSELLLVEEIPR 563
Cdd:PRK07529 474 GGHNIDPAAIEEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGASATEAELLAFARDhIAERAAVPKHVRILDALPK 553
|
410
....*....|.
gi 743747175 564 NQMGKVNKRDL 574
Cdd:PRK07529 554 TAVGKIFKPAL 564
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
48-578 |
1.31e-57 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 202.29 E-value: 1.31e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 48 TRALAFGDRVALADQHGShipagSHTYRDLYSRSLSLAReicRLRAC--SDGDlreeRVSFLCSNDVSYVVAQWASWMSG 125
Cdd:PRK06087 31 QTARAMPDKIAVVDNHGA-----SYTYSALDHAASRLAN---WLLAKgiEPGD----RVAFQLPGWCEFTIIYLACLKVG 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 126 GTAVPLYRKHPQAELEYFIQDSRSSV---------------VLAGQEHVELLSPVV--RKLGVPLLPLPPTVYCgAAKDP 188
Cdd:PRK06087 99 AVSVPLLPSWREAELVWVLNKCQAKMffaptlfkqtrpvdlILPLQNQLPQLQQIVgvDKLAPATSSLSLSQII-ADYEP 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 189 GEEQVPERDwrDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGA 268
Cdd:PRK06087 178 LTTAITTHG--DELAAVLFTSGTEGLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGA 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 269 TCVMLPEFSAQLVWEkfLSSQSPRVNVFMAVPTIYS--KLMDYHDRHFgqphvrdfvravceEKIRLMVSGSAALPLPVL 346
Cdd:PRK06087 256 RSVLLDIFTPDACLA--LLEQQRCTCMLGATPFIYDllNLLEKQPADL--------------SALRFFLCGGTTIPKKVA 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 347 EKWKGAtGHTLLERYGMTEigmalSNPLA-------TARLPGSVGTPLPGVEVRIVSENPQKdgcpyiihaegnetetrV 419
Cdd:PRK06087 320 RECQQR-GIKLLSVYGSTE-----SSPHAvvnlddpLSRFMHTDGYAAAGVEIKVVDEARKT-----------------L 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 420 TPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLL 498
Cdd:PRK06087 377 PPG---CEGEEASRGPNVFMGYLDEPELTARALDEEGWYYSGDLCRMdEAGYIKITGRKK-DIIVRGGENISSREVEDIL 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 499 LGHPSIADVAVIGVPDVTWGQRVTAVVTLREG-HSLSHRELKEW-ARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQ 576
Cdd:PRK06087 453 LQHPKIHDACVVAMPDERLGERSCAYVVLKAPhHSLTLEEVVAFfSRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRK 532
|
..
gi 743747175 577 QL 578
Cdd:PRK06087 533 DI 534
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
125-574 |
1.33e-57 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 201.45 E-value: 1.33e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 125 GGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQEhvelLSPVVRKLGVPLLPLPPTVYCGAAKDPGEE-----------QV 193
Cdd:PRK08008 86 GAIMVPINARLLREESAWILQNSQASLLVTSAQ----FYPMYRQIQQEDATPLRHICLTRVALPADDgvssftqlkaqQP 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 194 PERDWR-----DRGAMIIYTSGTTGRPKGVVITHHNVwaTVTGLVHKW--AWTKDDVILHVLPLHHVHGVVNKLLCPLWV 266
Cdd:PRK08008 162 ATLCYApplstDDTAEILFTSGTTSRPKGVVITHYNL--RFAGYYSAWqcALRDDDVYLTVMPAFHIDCQCTAAMAAFSA 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 267 GATCVMLPEFSAQLVWEKFlssQSPRVNVFMAVPTIYSKLMdyhdrhfGQPhVRDFVRAVCeekIRLMVsgsAALPLPVL 346
Cdd:PRK08008 240 GATFVLLEKYSARAFWGQV---CKYRATITECIPMMIRTLM-------VQP-PSANDRQHC---LREVM---FYLNLSDQ 302
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 347 EK--WKGATGHTLLERYGMTE-IGMALSNPLATARLPGSVGTPLPGVEVRIVSENpqkdgcpyiihaeGNEtetrVTPGf 423
Cdd:PRK08008 303 EKdaFEERFGVRLLTSYGMTEtIVGIIGDRPGDKRRWPSIGRPGFCYEAEIRDDH-------------NRP----LPAG- 364
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 424 eeTEGELLVR---GPSVFRGYWDKPEETKNAFTSDGWFKTGDTA-VFKDGSYWIRGRtSVDIIKTGGYKVSALEVERLLL 499
Cdd:PRK08008 365 --EIGEICIKgvpGKTIFKEYYLDPKATAKVLEADGWLHTGDTGyVDEEGFFYFVDR-RCNMIKRGGENVSCVELENIIA 441
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 743747175 500 GHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK08008 442 THPKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEEEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNL 516
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
73-577 |
2.26e-57 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 201.27 E-value: 2.26e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRlracsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PRK05852 45 SYRDLARLVDDLAGQLTR-----SGLLPGDRVALRMGSNAEFVVALLAASRADLVVVPLDPALPIAEQRVRSQAAGARVV 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHV-ELLSPVVRKLGV--------PLLPLPPTVYCGAAKDPGEEQVPERDWRDRGAMIIYTSGTTGRPKGVVITHH 223
Cdd:PRK05852 120 LIDADGPhDRAEPTTRWWPLtvnvggdsGPSGGTLSVHLDAATEPTPATSTPEGLRPDDAMIMFTGGTTGLPKMVPWTHA 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 224 NVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATcVMLP---EFSAQLVWEKFLSSQSprvNVFMAVP 300
Cdd:PRK05852 200 NIASSVRAIITGYRLSPRDATVAVMPLYHGHGLIAALLATLASGGA-VLLPargRFSAHTFWDDIKAVGA---TWYTAVP 275
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 301 TIYSKLMDYHD-RHFGQPHvrdfvravceEKIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIgmalSNPLATARL 379
Cdd:PRK05852 276 TIHQILLERAAtEPSGRKP----------AALRFIRSCSAPLTAETAQALQTEFAAPVVCAFGMTEA----THQVTTTQI 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 380 PGSVGTPLPGVEVRIV--SENPQKDgcpyIIHAEGNEtetrVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTsDGW 457
Cdd:PRK05852 342 EGIGQTENPVVSTGLVgrSTGAQIR----IVGSDGLP----LPAG---AVGEVWLRGTTVVRGYLGDPTITAANFT-DGW 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 458 FKTGDT-AVFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHR 536
Cdd:PRK05852 410 LRTGDLgSLSAAGDLSIRGRIK-ELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVAAVIVPRESAPPTAE 488
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 743747175 537 ELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQ 577
Cdd:PRK05852 489 ELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRRAVAEQ 529
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
73-510 |
2.74e-57 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 199.21 E-value: 2.74e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDL--YSRSLSLAREICRLRacsdgdlREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSS 150
Cdd:cd05914 9 TYKDLadNIAKFALLLKINGVG-------TGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAK 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 151 VVLAGQEhvellspvvrklgvpllplpptvycgaakdpgeeqvperdwrDRGAMIIYTSGTTGRPKGVVITHHNVWATVT 230
Cdd:cd05914 82 AIFVSDE------------------------------------------DDVALINYTSGTTGNSKGVMLTYRNIVSNVD 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 231 GLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQL-----------------VWE---KFLSSQS 290
Cdd:cd05914 120 GVKEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDKIPSAKiialafaqvtptlgvpvPLViekIFKMDII 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 291 PRVNV-----FMAVP--------TIYSKLMDyhdrHFGQphvrdfvravceeKIRLMVSGSAALPLPVLEKWKGAtGHTL 357
Cdd:cd05914 200 PKLTLkkfkfKLAKKinnrkirkLAFKKVHE----AFGG-------------NIKEFVIGGAKINPDVEEFLRTI-GFPY 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 358 LERYGMTEIGMALS-NPLATARLpGSVGTPLPGVEVRIVSENPqkdgcpyiihaegnetetrvtpgfEETEGELLVRGPS 436
Cdd:cd05914 262 TIGYGMTETAPIISySPPNRIRL-GSAGKVIDGVEVRIDSPDP------------------------ATGEGEIIVRGPN 316
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 743747175 437 VFRGYWDKPEETKNAFTSDGWFKTGDTAVFKDGSY-WIRGRTSVDIIKTGGYKVSALEVERLLLGHPSIADVAVI 510
Cdd:cd05914 317 VMKGYYKNPEATAEAFDKDGWFHTGDLGKIDAEGYlYIRGRKKEMIVLSSGKNIYPEEIEAKINNMPFVLESLVV 391
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
205-571 |
1.97e-55 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 190.40 E-value: 1.97e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITHHNV------WATVTGLvhkwawTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSA 278
Cdd:cd17638 5 IMFTSGTTGRSKGVMCAHRQTlraaaaWADCADL------TEDDRYLIINPFFHTFGYKAGIVACLLTGATVVPVAVFDV 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 QLVWEKFlssQSPRVNVFMAVPTIYSKLMDYhdrhfgqPHVRDFVRAvceeKIRLMVSGSAALPLPVLEKWKGATG-HTL 357
Cdd:cd17638 79 DAILEAI---ERERITVLPGPPTLFQSLLDH-------PGRKKFDLS----SLRAAVTGAATVPVELVRRMRSELGfETV 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 358 LERYGMTEIGMA-LSNPLATARLPGS-VGTPLPGVEVRIVSEnpqkdgcpyiihaegnetetrvtpgfeeteGELLVRGP 435
Cdd:cd17638 145 LTAYGLTEAGVAtMCRPGDDAETVATtCGRACPGFEVRIADD------------------------------GEVLVRGY 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 436 SVFRGYWDKPEETKNAFTSDGWFKTGDTAVFKDGSYW-IRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPD 514
Cdd:cd17638 195 NVMQGYLDDPEATAEAIDADGWLHTGDVGELDERGYLrITDRLK-DMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPD 273
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*..
gi 743747175 515 VTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNK 571
Cdd:cd17638 274 ERMGEVGKAFVVARPGVTLTEEDVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
53-579 |
2.11e-55 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 195.65 E-value: 2.11e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 53 FGDRVALAdqHGshipAGSHTYRDLYSRSLSLAREICRlRACSDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTAVPL- 131
Cdd:PRK07470 20 FPDRIALV--WG----DRSWTWREIDARVDALAAALAA-RGVRKGD----RILVHSRNCNQMFESMFAAFRLGAVWVPTn 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 132 YRKHPqAELEYFIQDSRSSVVLAG---QEHVEL---LSPVVR-KLGVPLLPLPPTVYCGAAKDPGEEQVPERDWRDRGAM 204
Cdd:PRK07470 89 FRQTP-DEVAYLAEASGARAMICHadfPEHAAAvraASPDLThVVAIGGARAGLDYEALVARHLGARVANAAVDHDDPCW 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITHHNVWATVT--------GLvhkwawTKDDVILHVLPLHHVHGVvnKLLCPLWVGATCVMLP-- 274
Cdd:PRK07470 168 FFFTSGTTGRPKAAVLTHGQMAFVITnhladlmpGT------TEQDASLVVAPLSHGAGI--HQLCQVARGAATVLLPse 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 275 EFSAQLVW---EKFlssqspRVNVFMAVPTIYsKLM------DYHD----RHF---GQPHVR-DFVRAvceekirlmvsg 337
Cdd:PRK07470 240 RFDPAEVWalvERH------RVTNLFTVPTIL-KMLvehpavDRYDhsslRYViyaGAPMYRaDQKRA------------ 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 338 saalpLPVLekwkgatGHTLLERYGMTEI---------GMALSNPLATARLpGSVGTPLPGVEVRIvsenpQKDgcpyii 408
Cdd:PRK07470 301 -----LAKL-------GKVLVQYFGLGEVtgnitvlppALHDAEDGPDARI-GTCGFERTGMEVQI-----QDD------ 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 409 haEGNEtetrVTPGfeETeGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTA-VFKDGSYWIRGRTSvDIIKTGGY 487
Cdd:PRK07470 357 --EGRE----LPPG--ET-GEICVIGPAVFAGYYNNPEANAKAF-RDGWFRTGDLGhLDARGFLYITGRAS-DMYISGGS 425
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 488 KVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMG 567
Cdd:PRK07470 426 NVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVDEAELLAWLDGKVARYKLPKRFFFWDALPKSGYG 505
|
570
....*....|..
gi 743747175 568 KVNKRDLVQQLY 579
Cdd:PRK07470 506 KITKKMVREELE 517
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
37-577 |
1.06e-53 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 191.53 E-value: 1.06e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 37 AARGNRSAPVFTRALAFGDRVALadqhgsHIPAGSHTYRDLYSRSLSLAREICRlRACSDGDlreeRVSFLCSNDVSYVV 116
Cdd:PRK07786 14 ARRQNWVNQLARHALMQPDAPAL------RFLGNTTTWRELDDRVAALAGALSR-RGVGFGD----RVLILMLNRTEFVE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 117 AQWASWMSGGTAVPL-YRKHPqAELEYFIQDSRSSVVLAgqEHVelLSPVVRKLGVPLLPLPPTVYCGAAKDPG----EE 191
Cdd:PRK07786 83 SVLAANMLGAIAVPVnFRLTP-PEIAFLVSDCGAHVVVT--EAA--LAPVATAVRDIVPLLSTVVVAGGSSDDSvlgyED 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 192 QVPERDW--------RDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKW-AWTKDDVILHVLPLHHVHGVVNkLLC 262
Cdd:PRK07786 158 LLAEAGPahapvdipNDSPALIMYTSGTTGRPKGAVLTHANLTGQAMTCLRTNgADINSDVGFVGVPLFHIAGIGS-MLP 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 263 PLWVGATCVMLP--EFSA-QL--VWEkflssqSPRVNVFMAVPTIYsklmdyhdrhfgqphvrdfvRAVCEE-------- 329
Cdd:PRK07786 237 GLLLGAPTVIYPlgAFDPgQLldVLE------AEKVTGIFLVPAQW--------------------QAVCAEqqarprdl 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 330 KIRLMVSGSAALPLPVLEKWKGA-TGHTLLERYGMTE---IGMALSNPLATARLpGSVGTPLPGVEVRIVSENPQKdgcp 405
Cdd:PRK07786 291 ALRVLSWGAAPASDTLLRQMAATfPEAQILAAFGQTEmspVTCMLLGEDAIRKL-GSVGKVIPTVAARVVDENMND---- 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 406 yiihaegnetetrVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSdGWFKTGDTA-VFKDGSYWIRGRTSvDIIKT 484
Cdd:PRK07786 366 -------------VPVG---EVGEIVYRAPTLMSGYWNNPEATAEAFAG-GWFHSGDLVrQDEEGYVWVVDRKK-DMIIS 427
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 485 GGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLR-EGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPR 563
Cdd:PRK07786 428 GGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRnDDAALTLEDLAEFLTDRLARYKHPKALEIVDALPR 507
|
570
....*....|....
gi 743747175 564 NQMGKVNKRDLVQQ 577
Cdd:PRK07786 508 NPAGKVLKTELRER 521
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
48-574 |
3.17e-53 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 188.69 E-value: 3.17e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 48 TRALAFGDRVALADqhgshiPAGSHTYRDLYSRSLSLAreiCRLRacSDGDLREERVSFLCSNDVSYVVAQWASWMSGGT 127
Cdd:cd05920 23 RSAARHPDRIAVVD------GDRRLTYRELDRRADRLA---AGLR--GLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAV 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 128 ---AVPLYRKHpqaELEYFIQDSRSSVVL-----AGQEHVELLSPVVRKLGvpllplpptvycgaakDPgeeqvperdwr 199
Cdd:cd05920 92 pvlALPSHRRS---ELSAFCAHAEAVAYIvpdrhAGFDHRALARELAESIP----------------EV----------- 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 200 drgAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHvhgvvN-KLLCP-----LWVGATCVML 273
Cdd:cd05920 142 ---ALFLLSGGTTGTPKLIPRTHNDYAYNVRASAEVCGLDQDTVYLAVLPAAH-----NfPLACPgvlgtLLAGGRVVLA 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 274 PEFSAQLVwekFLSSQSPRVNVFMAVPTIYSKLMDYHDRHFGQPhvrdfvravceEKIRLMVSGSAALPLPVLEKWKGAT 353
Cdd:cd05920 214 PDPSPDAA---FPLIEREGVTVTALVPALVSLWLDAAASRRADL-----------SSLRLLQVGGARLSPALARRVPPVL 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 354 GHTLLERYGMTEiGM----ALSNPlaTARLPGSVGTPL-PGVEVRIVSEnpqkdgcpyiihaEGNEtetrVTPGfeeTEG 428
Cdd:cd05920 280 GCTLQQVFGMAE-GLlnytRLDDP--DEVIIHTQGRPMsPDDEIRVVDE-------------EGNP----VPPG---EEG 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 429 ELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTA-VFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADV 507
Cdd:cd05920 337 ELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVrRTPDGYLVVEGRIK-DQINRGGEKIAAEEVENLLLRHPAVHDA 415
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 743747175 508 AVIGVPDVTWGQRVTAVVTLReGHSLSHRELKEWARGV-LAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05920 416 AVVAMPDELLGERSCAFVVLR-DPPPSAAQLRRFLRERgLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
200-574 |
5.38e-53 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 184.61 E-value: 5.38e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 200 DRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGAtcvmlpefsaQ 279
Cdd:cd05944 2 DDVAAYFHTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGA----------H 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 280 LVWEKFLSSQSP-------------RVNVFMAVPTIYSKLMDyhdrhfgQPHVRDFvravceEKIRLMVSGSAALPLPVL 346
Cdd:cd05944 72 VVLAGPAGYRNPglfdnfwklveryRITSLSTVPTVYAALLQ-------VPVNADI------SSLRFAMSGAAPLPVELR 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 347 EKWKGATGHTLLERYGMTEIGMALS-NPLATARLPGSVGTPLPGVEVRIVSENPQKDgcpYIIHAEGNETetrvtpgfee 425
Cdd:cd05944 139 ARFEDATGLPVVEGYGLTEATCLVAvNPPDGPKRPGSVGLRLPYARVRIKVLDGVGR---LLRDCAPDEV---------- 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 426 teGELLVRGPSVFRGYWDKpEETKNAFTSDGWFKTGDTA-VFKDGSYWIRGRtSVDIIKTGGYKVSALEVERLLLGHPSI 504
Cdd:cd05944 206 --GEICVAGPGVFGGYLYT-EGNKNAFVADGWLNTGDLGrLDADGYLFITGR-AKDLIIRGGHNIDPALIEEALLRHPAV 281
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 743747175 505 ADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPY-AVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05944 282 AFAGAVGQPDAHAGELPVAYVQLKPGAVVEEEELLAWARDHVPERaAVPKHIEVLEELPVTAVGKVFKPAL 352
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
73-578 |
2.83e-52 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 186.63 E-value: 2.83e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLArEICRLRACSDGDLreerVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PRK06145 29 SYAEFHQRILQAA-GMLHARGIGQGDV----VALLMKNSAAFLELAFAASYLGAVFLPINYRLAADEVAYILGDAGAKLL 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHVELLSPVVRKLGVPLLPLPPTVYCGAAKDPGEEQVPERDwrDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGL 232
Cdd:PRK06145 104 LVDEEFDAIVALETPKIVIDAAAQADSRRLAQGGLEIPPQAAVAP--TDLVRLMYTSGTTDRPKGVMHSYGNLHWKSIDH 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 233 VHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWEkflSSQSPRVNVFMAVPTIYSKLMDYHDR 312
Cdd:PRK06145 182 VIALGLTASERLLVVGPLYHVGAFDLPGIAVLWVGGTLRIHREFDPEAVLA---AIERHRLTCAWMAPVMLSRVLTVPDR 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 313 HfgqphvRDFVravceEKIRLMVSGSAALP-LPVLEKWKGATGHTLLERYGMTEI--GMALSNPLATARLPGSVGTPLPG 389
Cdd:PRK06145 259 D------RFDL-----DSLAWCIGGGEKTPeSRIRDFTRVFTRARYIDAYGLTETcsGDTLMEAGREIEKIGSTGRALAH 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 390 VEVRIVSEnpqkdgcpyiihaegnetETRVTPgfEETEGELLVRGPSVFRGYWDKPEETKNAFTsDGWFKTGDTAVFKD- 468
Cdd:PRK06145 328 VEIRIADG------------------AGRWLP--PNMKGEICMRGPKVTKGYWKDPEKTAEAFY-GDWFRSGDVGYLDEe 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 469 GSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAP 548
Cdd:PRK06145 387 GFLYLTDRKK-DMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGATLTLEALDRHCRQRLAS 465
|
490 500 510
....*....|....*....|....*....|
gi 743747175 549 YAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK06145 466 FKVPRQLKVRDELPRNPSGKVLKRVLRDEL 495
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
201-578 |
5.50e-52 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 180.99 E-value: 5.50e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 201 RGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCpLWVGATCVMLPEFSAQL 280
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWLLSLPLYHVGGLAILVRS-LLAGAELVLLERNQALA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 281 VwekflSSQSPRVNVFMAVPTIYSKLMDYHdrhfGQPHVRDFVRAVceekirlmVSGSAALPLPVLEKWKGAtGHTLLER 360
Cdd:cd17630 80 E-----DLAPPGVTHVSLVPTQLQRLLDSG----QGPAALKSLRAV--------LLGGAPIPPELLERAADR-GIPLYTT 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 361 YGMTEIG--MALSNPLATARlpGSVGTPLPGVEVRIVSEnpqkdgcpyiihaegnetetrvtpgfeeteGELLVRGPSVF 438
Cdd:cd17630 142 YGMTETAsqVATKRPDGFGR--GGVGVLLPGRELRIVED------------------------------GEIWVGGASLA 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 439 RGYWDKPEEtkNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTW 517
Cdd:cd17630 190 MGYLRGQLV--PEFNEDGWFTTKDLGELhADGRLTVLGRAD-NMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEEL 266
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 743747175 518 GQRVTAVVTLREGHSLShrELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:cd17630 267 GQRPVAVIVGRGPADPA--ELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
102-578 |
6.19e-52 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 187.17 E-value: 6.19e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 102 ERVSFLCSNDVSYVVAQWASWMSGGTAVP---LYRKHpqaELEYFIQDSRSSVVLAgqehVELLSPVVRKlgVPLLPLPP 178
Cdd:PRK06178 84 DRVAVFLPNCPQFHIVFFGILKLGAVHVPvspLFREH---ELSYELNDAGAEVLLA----LDQLAPVVEQ--VRAETSLR 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 179 TVYCGAAKD--PGEEQVPERD-----------WRD-----RG---------------AMIIYTSGTTGRPKGVVITH-HN 224
Cdd:PRK06178 155 HVIVTSLADvlPAEPTLPLPDslraprlaaagAIDllpalRActapvplpppaldalAALNYTGGTTGMPKGCEHTQrDM 234
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 225 VW--ATVTGLVHkwAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWEkflSSQSPRVNV-FMAVPT 301
Cdd:PRK06178 235 VYtaAAAYAVAV--VGGEDSVFLSFLPEFWIAGENFGLLFPLFSGATLVLLARWDAVAFMA---AVERYRVTRtVMLVDN 309
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 302 iYSKLMDYhdrhfgqPHVRDF-------VRAVceekirlmvSGSAALPLPVLEKWKGATGHTLLE-RYGMTE-------- 365
Cdd:PRK06178 310 -AVELMDH-------PRFAEYdlsslrqVRVV---------SFVKKLNPDYRQRWRALTGSVLAEaAWGMTEthtcdtft 372
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 366 IGMA------LSNPLAtarlpgsVGTPLPGVEVRIVSEnpqkdgcpyiihaegnETETRVTPGfeeTEGELLVRGPSVFR 439
Cdd:PRK06178 373 AGFQdddfdlLSQPVF-------VGLPVPGTEFKICDF----------------ETGELLPLG---AEGEIVVRTPSLLK 426
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 440 GYWDKPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWG 518
Cdd:PRK06178 427 GYWNKPEATAEAL-RDGWLHTGDIGKIdEQGFLHYLGRRK-EMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKG 504
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 519 QRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPsELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK06178 505 QVPVAFVQLKPGADLTAAALQAWCRENMAVYKVP-EIRIVDALPMTATGKVRKQDLQALA 563
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
55-574 |
9.97e-52 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 183.99 E-value: 9.97e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADQhgshipAGSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRK 134
Cdd:cd05945 6 DRPAVVEG------GRTLTYRELKERADALAAALASL-----GLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDAS 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 135 HPQAELEYFIQDSRSSVVLAgqehvellspvvrklgvpllplpptvycgaakDPGEEqvperdwrdrgAMIIYTSGTTGR 214
Cdd:cd05945 75 SPAERIREILDAAKPALLIA--------------------------------DGDDN-----------AYIIFTSGSTGR 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 215 PKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLH---HVHGvvnkLLCPLWVGATCVMLPEfsAQLVWEKFLSSQSP 291
Cdd:cd05945 112 PKGVQISHDNLVSFTNWMLSDFPLGPGDVFLNQAPFSfdlSVMD----LYPALASGATLVPVPR--DATADPKQLFRFLA 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 292 R--VNVFMAVPTIYSKLMdyHDRHFGQ---PHVRDFVraVCEEkirlmvsgsaALPLPVLEKWKGAT-GHTLLERYGMTE 365
Cdd:cd05945 186 EhgITVWVSTPSFAAMCL--LSPTFTPeslPSLRHFL--FCGE----------VLPHKTARALQQRFpDARIYNTYGPTE 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 366 IGMALS------NPLATA-RLPgsVGTPLPGVEVrivsenpqkdgcpYIIHAEGNEtetrVTPGfeeTEGELLVRGPSVF 438
Cdd:cd05945 252 ATVAVTyievtpEVLDGYdRLP--IGYAKPGAKL-------------VILDEDGRP----VPPG---EKGELVISGPSVS 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 439 RGYWDKPEETKNAFTSD---GWFKTGDTAVFKDGSYWI-RGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPD 514
Cdd:cd05945 310 KGYLNNPEKTAAAFFPDegqRAYRTGDLVRLEADGLLFyRGRLD-FQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYK 388
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 743747175 515 VTWGQRVTAVVTLREGHSLSH-RELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05945 389 GEKVTELIAFVVPKPGAEAGLtKAIKAELAERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
35-574 |
1.84e-51 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 185.52 E-value: 1.84e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 35 GPAARGNRSApvftrALAFGDRVALADQHGSHipagshTYRDLYSRSLSLAREICRlRACSDGDlreeRVSFLCSNDVSY 114
Cdd:PRK07788 49 GPFAGLVAHA-----ARRAPDRAALIDERGTL------TYAELDEQSNALARGLLA-LGVRAGD----GVAVLARNHRGF 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 115 VVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPLLPlpptvycGAAKDPGEEQVP 194
Cdd:PRK07788 113 VLALYAAGKVGARIILLNTGFSGPQLAEVAAREGVKALVYDDEFTDLLSALPPDLGRLRAW-------GGNPDDDEPSGS 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 195 ERD----------------WRDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVN 258
Cdd:PRK07788 186 TDEtlddliagsstaplpkPPKPGGIVILTSGTTGTPKGAPRPEPSPLAPLAGLLSRVPFRAGETTLLPAPMFHATGWAH 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 259 KLLCpLWVGATCVMLPEFSAQLVWEkflSSQSPRVNVFMAVPTIYSKLMDYHDRHFGQPHVRdfvravceeKIRLMVSGS 338
Cdd:PRK07788 266 LTLA-MALGSTVVLRRRFDPEATLE---DIAKHKATALVVVPVMLSRILDLGPEVLAKYDTS---------SLKIIFVSG 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 339 AALPLPVLEKWKGATGHTLLERYGMTEIGMA-LSNPLATARLPGSVGTPLPGVEVRIVSENPQkdgcpyiihaegnetet 417
Cdd:PRK07788 333 SALSPELATRALEAFGPVLYNLYGSTEVAFAtIATPEDLAEAPGTVGRPPKGVTVKILDENGN----------------- 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 418 RVTPGfeeTEGELLVRGPSVFRGYWDkpeeTKNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVER 496
Cdd:PRK07788 396 EVPRG---VVGRIFVGNGFPFEGYTD----GRDKQIIDGLLSSGDVGYFdEDGLLFVDGRDD-DMIVSGGENVFPAEVED 467
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 743747175 497 LLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK07788 468 LLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKAPGAALDEDAIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKREL 545
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
54-574 |
1.58e-50 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 180.95 E-value: 1.58e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 54 GDRVALADQHGSHipagshTYRDLYSRSLSLAReicRLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYR 133
Cdd:cd12116 1 PDATAVRDDDRSL------SYAELDERANRLAA---RLRA--RGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDP 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 134 KHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPLLPLPptvycGAAKDPGEEQVPERDwrdrgAMIIYTSGTTG 213
Cdd:cd12116 70 DYPADRLRYILEDAEPALVLTDDALPDRLPAGLPVLLLALAAAA-----AAPAAPRTPVSPDDL-----AYVIYTSGSTG 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 214 RPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLP-------LhhvhgvvnKLLCPLWVGATCVML-------PEFSAQ 279
Cdd:cd12116 140 RPKGVVVSHRNLVNFLHSMRERLGLGPGDRLLAVTTyafdislL--------ELLLPLLAGARVVIApretqrdPEALAR 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 280 LVwekflssQSPRVNVFMAVPTIYSKLMDyhdrhfGQPHVRDFVRAVCeekirlmvsGSAALPlPVLEKWKGATGHTLLE 359
Cdd:cd12116 212 LI-------EAHSITVMQATPATWRMLLD------AGWQGRAGLTALC---------GGEALP-PDLAARLLSRVGSLWN 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 360 RYGMTEIGM-ALSNPLATARLPGSVGTPLPGVEVRIVSENPQKdgcpyiihaegnetetrVTPGfeeTEGELLVRGPSVF 438
Cdd:cd12116 269 LYGPTETTIwSTAARVTAAAGPIPIGRPLANTQVYVLDAALRP-----------------VPPG---VPGELYIGGDGVA 328
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 439 RGYWDKPEETKNAFTSDG-------WFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVI 510
Cdd:cd12116 329 QGYLGRPALTAERFVPDPfagpgsrLYRTGDLVRRRaDGRLEYLGRAD-GQVKIRGHRIELGEIEAALAAHPGVAQAAVV 407
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 743747175 511 GVPDvTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd12116 408 VRED-GGDRRLVAYVVLKAGAAPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
73-509 |
2.17e-50 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 179.00 E-value: 2.17e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLRACSdgdlREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAGGVG----PGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLL 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHVELLSPVVRKLGVPLLPLPPTvycgAAKDPGEEQVPERDWRDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGL 232
Cdd:TIGR01733 77 LTDSALASRLAGLVLPVILLDPLELAA----LDDAPAPPPPDAPSGPDDLAYVIYTSGSTGRPKGVVVTHRSLVNLLAWL 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 233 VHKWAWTKDDVILHVLPLHHVHGVVnKLLCPLWVGATCVMLPEfSAQLVWEKFLSS--QSPRVNVFMAVPTIYSKLMDyh 310
Cdd:TIGR01733 153 ARRYGLDPDDRVLQFASLSFDASVE-EIFGALLAGATLVVPPE-DEERDDAALLAAliAEHPVTVLNLTPSLLALLAA-- 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 311 DRHFGQPHVrdfvravceekiRLMVSGSAALPLPVLEKWKGATGHT-LLERYGMTE-----IGMALSNPLATARLPGSVG 384
Cdd:TIGR01733 229 ALPPALASL------------RLVILGGEALTPALVDRWRARGPGArLINLYGPTEttvwsTATLVDPDDAPRESPVPIG 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 385 TPLPGVEVRIVSEN--PQKDGCPyiihaegnetetrvtpgfeeteGELLVRGPSVFRGYWDKPEETKNAFTSDG------ 456
Cdd:TIGR01733 297 RPLANTRLYVLDDDlrPVPVGVV----------------------GELYIGGPGVARGYLNRPELTAERFVPDPfaggdg 354
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 457 --WFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAV 509
Cdd:TIGR01733 355 arLYRTGDLVRYlPDGNLEFLGRID-DQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
52-571 |
2.48e-50 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 182.67 E-value: 2.48e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 52 AFGDRVALADQHgshiPAGSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAV-- 129
Cdd:PRK12583 30 RFPDREALVVRH----QALRYTWRQLADAVDRLARGLLAL-----GVQPGDRVGIWAPNCAEWLLTQFATARIGAILVni 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 130 -PLYRKhpqAELEYFIQDSRSSVVLA-----GQEHVELLSPVVRKLGVPLLPLPP---------TVYCGAAKDPG----- 189
Cdd:PRK12583 101 nPAYRA---SELEYALGQSGVRWVICadafkTSDYHAMLQELLPGLAEGQPGALAcerlpelrgVVSLAPAPPPGflawh 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 190 -----EEQVPERDWRDRGAM--------IIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGV 256
Cdd:PRK12583 178 elqarGETVSREALAERQASldrddpinIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDRLCVPVPLYHCFGM 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 257 VNKLLCPLWVGAtCVMLP--EFSAQLVWEkflSSQSPRVNVFMAVPTIYSKLMDYHDR-HFgqphvrDFvravceEKIRL 333
Cdd:PRK12583 258 VLANLGCMTVGA-CLVYPneAFDPLATLQ---AVEEERCTALYGVPTMFIAELDHPQRgNF------DL------SSLRT 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 334 MVSGSAALPLPVLEKwkgatghtLLERYGMTEI----GMALSNPLATARLPG--------SVGTPLPGVEVRIVsenpqk 401
Cdd:PRK12583 322 GIMAGAPCPIEVMRR--------VMDEMHMAEVqiayGMTETSPVSLQTTAAddlerrveTVGRTQPHLEVKVV------ 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 402 dgcpyiiHAEGNetetrvTPGFEETeGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRtSVD 480
Cdd:PRK12583 388 -------DPDGA------TVPRGEI-GELCTRGYSVMKGYWNNPEATAESIDEDGWMHTGDLATMdEQGYVRIVGR-SKD 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 481 IIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEE 560
Cdd:PRK12583 453 MIIRGGENIYPREIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRLHPGHAASEEELREFCKARIAHFKVPRYFRFVDE 532
|
570
....*....|.
gi 743747175 561 IPRNQMGKVNK 571
Cdd:PRK12583 533 FPMTVTGKVQK 543
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
199-574 |
3.10e-50 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 182.18 E-value: 3.10e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 199 RDRGAMIIYTSGTTGRPKGVVITHHNVWATVtgLVHKWAW-----TKDDVILHVLPLHHVHGV-VNKLLCpLWVGATCVM 272
Cdd:PRK08974 205 PEDLAFLQYTGGTTGVAKGAMLTHRNMLANL--EQAKAAYgpllhPGKELVVTALPLYHIFALtVNCLLF-IELGGQNLL 281
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 273 ------LPEFSAQLvwekflssQSPRVNVFMAVPTIYSKLMdyHDRHFgqpHVRDFvravceEKIRLMVSGSAALPLPVL 346
Cdd:PRK08974 282 itnprdIPGFVKEL--------KKYPFTAITGVNTLFNALL--NNEEF---QELDF------SSLKLSVGGGMAVQQAVA 342
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 347 EKWKGATGHTLLERYGMTEIGMALS-NPLATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGNEtetrVTPGfeE 425
Cdd:PRK08974 343 ERWVKLTGQYLLEGYGLTECSPLVSvNPYDLDYYSGSIGLPVPSTEIKLVDD-------------DGNE----VPPG--E 403
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 426 TeGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSI 504
Cdd:PRK08974 404 P-GELWVKGPQVMLGYWQRPEATDEVI-KDGWLATGDIAVMdEEGFLRIVDRKK-DMILVSGFNVYPNEIEDVVMLHPKV 480
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 505 ADVAVIGVPDVTWGQRVTAVVTLREgHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK08974 481 LEVAAVGVPSEVSGEAVKIFVVKKD-PSLTEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRREL 549
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
200-576 |
4.28e-50 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 181.92 E-value: 4.28e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 200 DRGAMIIYTSGTTGRPKGVVITHHnvwATVTGLVHKWA---WTKDDVILHVLPLHHVHGVvNKLLCPLWVGATCVMLPEF 276
Cdd:PLN02860 172 DDAVLICFTSGTTGRPKGVTISHS---ALIVQSLAKIAivgYGEDDVYLHTAPLCHIGGL-SSALAMLMVGACHVLLPKF 247
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 277 SAQLVWEKFlssQSPRVNVFMAVPTIYSKLMDYhDRHFGQPHVRDFVRAvceekirlMVSGSAALPLPVLEKWKGATGHT 356
Cdd:PLN02860 248 DAKAALQAI---KQHNVTSMITVPAMMADLISL-TRKSMTWKVFPSVRK--------ILNGGGSLSSRLLPDAKKLFPNA 315
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 357 -LLERYGMTEIGMALS-NPLATARLPGSVGTPLPGVEVRIVSENPQKDGC-----PYIihaegnetETRVTPGFEETEGE 429
Cdd:PLN02860 316 kLFSAYGMTEACSSLTfMTLHDPTLESPKQTLQTVNQTKSSSVHQPQGVCvgkpaPHV--------ELKIGLDESSRVGR 387
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 430 LLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFKD-GSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVA 508
Cdd:PLN02860 388 ILTRGPHVMLGYWGQNSETASVLSNDGWLDTGDIGWIDKaGNLWLIGRSN-DRIKTGGENVYPEEVEAVLSQHPGVASVV 466
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 509 VIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGV---------------LAPYAVPSELLLVEE-IPRNQMGKVnKR 572
Cdd:PLN02860 467 VVGVPDSRLTEMVVACVRLRDGWIWSDNEKENAKKNLtlssetlrhhcreknLSRFKIPKLFVQWRKpFPLTTTGKI-RR 545
|
....
gi 743747175 573 DLVQ 576
Cdd:PLN02860 546 DEVR 549
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
55-574 |
9.20e-50 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 178.66 E-value: 9.20e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADQHgSHIpagshTYRDLYSRSLSLAReicRLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRK 134
Cdd:cd17643 2 EAVAVVDED-RRL-----TYGELDARANRLAR---TLRA--EGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPA 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 135 HPQAELEYFIQDSRSSVVLagqehvellspvvrklgvpllplpptvycGAAKDPgeeqvperdwrdrgAMIIYTSGTTGR 214
Cdd:cd17643 71 YPVERIAFILADSGPSLLL-----------------------------TDPDDL--------------AYVIYTSGSTGR 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 215 PKGVVITHHNVWATVTGLVHKWAWTKDDVilhVLPLHH---------VHGvvnkllcPLWVGATCVML-------PEFSA 278
Cdd:cd17643 108 PKGVVVSHANVLALFAATQRWFGFNEDDV---WTLFHSyafdfsvweIWG-------ALLHGGRLVVVpyevarsPEDFA 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 QLVWEKflssqspRVNVFMAVPTIYSKLMDYHDRHFGQPHvrdfvravceeKIRLMVSGSAALPLPVLEKWKGATGH--- 355
Cdd:cd17643 178 RLLRDE-------GVTVLNQTPSAFYQLVEAADRDGRDPL-----------ALRYVIFGGEALEAAMLRPWAGRFGLdrp 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 356 TLLERYGMTEIGMALS-NPLATARLPGS----VGTPLPGVEVRIVSENPQkdgcpyiihaegnetetRVTPGfeeTEGEL 430
Cdd:cd17643 240 QLVNMYGITETTVHVTfRPLDAADLPAAaaspIGRPLPGLRVYVLDADGR-----------------PVPPG---VVGEL 299
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 431 LVRGPSVFRGYWDKPEETKNAFTSDGW-------FKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHP 502
Cdd:cd17643 300 YVSGAGVARGYLGRPELTAERFVANPFggpgsrmYRTGDLARRLpDGELEYLGRAD-EQVKIRGFRIELGEIEAALATHP 378
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 743747175 503 SIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17643 379 SVRDAAVIVREDEPGDTRLVAYVVADDGAAADIAELRALLKELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
37-574 |
1.29e-49 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 179.59 E-value: 1.29e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 37 AARGNRSAPvftrALAFGDRvaladqhgshipagSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVV 116
Cdd:TIGR03098 9 AAARLPDAT----ALVHHDR--------------TLTYAALSERVLALASGLRGL-----GLARGERVAIYLDKRLETVT 65
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 117 AQWASWMSGGTAVPL--YRKHPQaeLEYFIQDSRSSVVLAGQEHVELLSPV------VRKLGVPLLPLPPTVycgaaKDP 188
Cdd:TIGR03098 66 AMFGAALAGGVFVPInpLLKAEQ--VAHILADCNVRLLVTSSERLDLLHPAlpgchdLRTLIIVGDPAHASE-----GHP 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 189 GEEQV-------------PERDWRDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHG 255
Cdd:TIGR03098 139 GEEPAswpkllalgdadpPHPVIDSDMAAILYTSGSTGRPKGVVLSHRNLVAGAQSVATYLENRPDDRLLAVLPLSFDYG 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 256 vVNKLLCPLWVGATCVMLPEFSAQLVWEkflSSQSPRVNVFMAVPTIYSKLMDYHDRHFGQPHVRdfvravceekiRLMV 335
Cdd:TIGR03098 219 -FNQLTTAFYVGATVVLHDYLLPRDVLK---ALEKHGITGLAAVPPLWAQLAQLDWPESAAPSLR-----------YLTN 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 336 SGsAALPLPVLEKWKGATGHT-LLERYGMTEIGMALS-NPLATARLPGSVGTPLPGVEVRIVsenpqkdgcpyiiHAEGN 413
Cdd:TIGR03098 284 SG-GAMPRATLSRLRSFLPNArLFLMYGLTEAFRSTYlPPEEVDRRPDSIGKAIPNAEVLVL-------------REDGS 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 414 ETEtrvtPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTS----DGWFKTGDTAVF--------KDGSYWIRGRTSvDI 481
Cdd:TIGR03098 350 ECA----PG---EEGELVHRGALVAMGYWNDPEKTAERFRPlppfPGELHLPELAVWsgdtvrrdEEGFLYFVGRRD-EM 421
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 482 IKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEI 561
Cdd:TIGR03098 422 IKTSGYRVSPTEVEEVAYATGLVAEAVAFGVPDPTLGQAIVLVVTPPGGEELDRAALLAECRARLPNYMVPALIHVRQAL 501
|
570
....*....|...
gi 743747175 562 PRNQMGKVNKRDL 574
Cdd:TIGR03098 502 PRNANGKIDRKAL 514
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
203-574 |
2.92e-49 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 179.63 E-value: 2.92e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDD----------VILHVLPLHHVHGVVNKLLCPLWVGATCVM 272
Cdd:PRK12492 210 AVLQYTGGTTGLAKGAMLTHGNLVANMLQVRACLSQLGPDgqplmkegqeVMIAPLPLYHIYAFTANCMCMMVSGNHNVL 289
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 273 L------PEFSAQLVWEKFlssqsprvNVFMAVPTIYSKLMDYhdrhfgqPHVR--DFVRavceekIRLMVSGSAALPLP 344
Cdd:PRK12492 290 ItnprdiPGFIKELGKWRF--------SALLGLNTLFVALMDH-------PGFKdlDFSA------LKLTNSGGTALVKA 348
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 345 VLEKWKGATGHTLLERYGMTEIG-MALSNPLAT-ARLpGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGNETetrvtPG 422
Cdd:PRK12492 349 TAERWEQLTGCTIVEGYGLTETSpVASTNPYGElARL-GTVGIPVPGTALKVIDD-------------DGNEL-----PL 409
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 423 FEEteGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGH 501
Cdd:PRK12492 410 GER--GELCIKGPQVMKGYWQQPEATAEALDAEGWFKTGDIAVIdPDGFVRIVDRKK-DLIIVSGFNVYPNEIEDVVMAH 486
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 743747175 502 PSIADVAVIGVPDVTWGQRVTAVVTLREGhSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK12492 487 PKVANCAAIGVPDERSGEAVKLFVVARDP-GLSVEELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRREL 558
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
73-576 |
9.29e-49 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 175.70 E-value: 9.29e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAReicRLRacSDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:cd05971 8 TFKELKTASNRFAN---VLK--EIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASAL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAgqehvellspvvrklgvpllplpptvycgaakdpgeeqvperDWRDRGAMIIYTSGTTGRPKGVVITH-----HNV-- 225
Cdd:cd05971 83 VT------------------------------------------DGSDDPALIIYTSGTTGPPKGALHAHrvllgHLPgv 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 226 --------------WATVtglvhKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQlvwekflssqsp 291
Cdd:cd05971 121 qfpfnlfprdgdlyWTPA-----DWAWIGGLLDVLLPSLYFGVPVLAHRMTKFDPKAALDLMSRYGVT------------ 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 292 rvNVFMavPTIYSKLMDYHDRHFGQPHVrdfvravceeKIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIGMALS 371
Cdd:cd05971 184 --TAFL--PPTALKMMRQQGEQLKHAQV----------KLRAIATGGESLGEELLGWAREQFGVEVNEFYGQTECNLVIG 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 372 NPLATARL-PGSVGTPLPGVEVRIVSENpqkdgcpyiihaegnetETRVTPGfeeTEGELLVRGPS--VFRGYWDKPEET 448
Cdd:cd05971 250 NCSALFPIkPGSMGKPIPGHRVAIVDDN-----------------GTPLPPG---EVGEIAVELPDpvAFLGYWNNPSAT 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 449 KNAFTSDgWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTL 527
Cdd:cd05971 310 EKKMAGD-WLLTGDLGRKdSDGYFWYVGRDD-DVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVL 387
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 743747175 528 REGHSLSH---RELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQ 576
Cdd:cd05971 388 NPGETPSDalaREIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELRA 439
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
52-572 |
3.27e-48 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 176.23 E-value: 3.27e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 52 AFGDRVALAdqHGSHIpagsHTYRDLYSRSLSLAReicrlrACSDGDLR-EERVSFLCSNDVSYVVAQWASWMSGGTAVP 130
Cdd:PRK07798 15 AVPDRVALV--CGDRR----LTYAELEERANRLAH------YLIAQGLGpGDHVGIYARNRIEYVEAMLGAFKARAVPVN 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 131 L-YRKHPqAELEYFIQDSRSSVVLAGQEHVELLSPV------VRKLGVPLLPLPPTVYCGAAK--DPGEEQVPERDWRDR 201
Cdd:PRK07798 83 VnYRYVE-DELRYLLDDSDAVALVYEREFAPRVAEVlprlpkLRTLVVVEDGSGNDLLPGAVDyeDALAAGSPERDFGER 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 202 GA---MIIYTSGTTGRPKGVVITHHNVWAT-------VTG--------LVHKWAWTKDDVILHVLPLHHVHGVVNKLLCp 263
Cdd:PRK07798 162 SPddlYLLYTGGTTGMPKGVMWRQEDIFRVllggrdfATGepiedeeeLAKRAAAGPGMRRFPAPPLMHGAGQWAAFAA- 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 264 LWVGATCVMLP--EFSAQLVWEkflSSQSPRVNVF------MAVPTIysklmdyhdRHFGQPHVRDFVRavceekIRLMV 335
Cdd:PRK07798 241 LFSGQTVVLLPdvRFDADEVWR---TIEREKVNVItivgdaMARPLL---------DALEARGPYDLSS------LFAIA 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 336 SGSAALPLPVLEKWKGATGH-TLLERYGMTEIG-MALSnplATARLPGSVGTPL--PGVEVRIVSENpqkdgcpyiihae 411
Cdd:PRK07798 303 SGGALFSPSVKEALLELLPNvVLTDSIGSSETGfGGSG---TVAKGAVHTGGPRftIGPRTVVLDED------------- 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 412 gnetETRVTPGfeETEGELLVRGPSVFRGYWDKPEETKNAF-TSDG--WFKTGDTA-VFKDGSYWIRGRTSVdIIKTGGY 487
Cdd:PRK07798 367 ----GNPVEPG--SGEIGWIARRGHIPLGYYKDPEKTAETFpTIDGvrYAIPGDRArVEADGTITLLGRGSV-CINTGGE 439
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 488 KVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMG 567
Cdd:PRK07798 440 KVFPEEVEEALKAHPDVADALVVGVPDERWGQEVVAVVQLREGARPDLAELRAHCRSSLAGYKVPRAIWFVDEVQRSPAG 519
|
....*
gi 743747175 568 KVNKR 572
Cdd:PRK07798 520 KADYR 524
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
50-580 |
4.07e-48 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 176.54 E-value: 4.07e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 50 ALAFGDRVALADQHgSHIpagSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAV 129
Cdd:PRK08315 26 AARYPDREALVYRD-QGL---RWTYREFNEEVDALAKGLLAL-----GIEKGDRVGIWAPNVPEWVLTQFATAKIGAILV 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 130 ---PLYRKHpqaELEYFIQDSRSS-VVLAGQ----EHVELLSPVVRKLGVPLLPLP---------PTVYCGAAKDPGEEQ 192
Cdd:PRK08315 97 tinPAYRLS---ELEYALNQSGCKaLIAADGfkdsDYVAMLYELAPELATCEPGQLqsarlpelrRVIFLGDEKHPGMLN 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 193 VPErdWRDRGAM--------------------IIYTSGTTGRPKGVVITHHNVW------ATVTGLVHKwawtkDDVILH 246
Cdd:PRK08315 174 FDE--LLALGRAvddaelaarqatldpddpinIQYTSGTTGFPKGATLTHRNILnngyfiGEAMKLTEE-----DRLCIP 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 247 VlPLHHVHGVVNKLLCPLWVGATCV-MLPEFSAQLVWEkflSSQSPRVNVFMAVPTIYSKLMDYHDRH-FGQPHVRDFVR 324
Cdd:PRK08315 247 V-PLYHCFGMVLGNLACVTHGATMVyPGEGFDPLATLA---AVEEERCTALYGVPTMFIAELDHPDFArFDLSSLRTGIM 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 325 AvceekirlmvsGSAAlPLPVLEKwkgatghtLLERYGMTEI----GMALSNPLATARLP--------GSVGTPLPGVEV 392
Cdd:PRK08315 323 A-----------GSPC-PIEVMKR--------VIDKMHMSEVtiayGMTETSPVSTQTRTddplekrvTTVGRALPHLEV 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 393 RIVSEnpqkdgcpyiihaEGNETetrVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSY 471
Cdd:PRK08315 383 KIVDP-------------ETGET---VPRG---EQGELCTRGYSVMKGYWNDPEKTAEAIDADGWMHTGDLAVMdEEGYV 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 472 WIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAV 551
Cdd:PRK08315 444 NIVGRIK-DMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTEEDVRDFCRGKIAHYKI 522
|
570 580 590
....*....|....*....|....*....|..
gi 743747175 552 PSELLLVEEIPRNQMGKVNK---RDLVQQLYP 580
Cdd:PRK08315 523 PRYIRFVDEFPMTVTGKIQKfkmREMMIEELG 554
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
46-578 |
4.29e-48 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 175.94 E-value: 4.29e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 46 VFTRALAFGDRVALADQhgshiPAG-SHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMS 124
Cdd:PLN02246 29 CFERLSEFSDRPCLIDG-----ATGrVYTYADVELLSRRVAAGLHKL-----GIRQGDVVMLLLPNCPEFVLAFLGASRR 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 125 GG---TAVPLYrkhPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPL----LPLPPTVYCGAAKDPGEEQVPERD 197
Cdd:PLN02246 99 GAvttTANPFY---TPAEIAKQAKASGAKLIITQSCYVDKLKGLAEDDGVTVvtidDPPEGCLHFSELTQADENELPEVE 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 198 WR-DRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVH----KWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVM 272
Cdd:PLN02246 176 ISpDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDgenpNLYFHSDDVILCVLPMFHIYSLNSVLLCGLRVGAAILI 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 273 LPEFSAQLVWEKFlssQSPRVNVFMAVPTIY-----SKLMDYHDRhfgqphvrdfvravceEKIRLMVSGSAalPL---- 343
Cdd:PLN02246 256 MPKFEIGALLELI---QRHKVTIAPFVPPIVlaiakSPVVEKYDL----------------SSIRMVLSGAA--PLgkel 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 344 --PVLEKWKGATghtLLERYGMTEIGMALSNPLATARLP-----GSVGTPLPGVEVRIVsenpqkdgcpyiihaegnETE 416
Cdd:PLN02246 315 edAFRAKLPNAV---LGQGYGMTEAGPVLAMCLAFAKEPfpvksGSCGTVVRNAELKIV------------------DPE 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 417 TRVTPGFEETeGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTA-VFKDGSYWIRGRTSvDIIKTGGYKVSALEVE 495
Cdd:PLN02246 374 TGASLPRNQP-GEICIRGPQIMKGYLNDPEATANTIDKDGWLHTGDIGyIDDDDELFIVDRLK-ELIKYKGFQVAPAELE 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 496 RLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLV 575
Cdd:PLN02246 452 ALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGSEITEDEIKQFVAKQVVFYKRIHKVFFVDSIPKAPSGKILRKDLR 531
|
...
gi 743747175 576 QQL 578
Cdd:PLN02246 532 AKL 534
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
199-574 |
4.84e-48 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 176.24 E-value: 4.84e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 199 RDRGAMIIYTSGTTGRPKGV------VITHHnvwatVTGlvhKWawtkddvilhVLPLHH------------VHGVVNKL 260
Cdd:PRK04319 204 REDGAILHYTSGSTGKPKGVlhvhnaMLQHY-----QTG---KY----------VLDLHEddvywctadpgwVTGTSYGI 265
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 261 LCPLWVGATCVML-PEFSAQlVWEKFLSSQspRVNVFMAVPTIYSKLMDYHDRHFGQ---PHVRdFVRAVCE----EKIR 332
Cdd:PRK04319 266 FAPWLNGATNVIDgGRFSPE-RWYRILEDY--KVTVWYTAPTAIRMLMGAGDDLVKKydlSSLR-HILSVGEplnpEVVR 341
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 333 LmvsGSAALPLPVLEKWkgatghtlleryGMTEIG-MALSNPLATARLPGSVGTPLPGVEVRIVSENpqkdgcpyiihae 411
Cdd:PRK04319 342 W---GMKVFGLPIHDNW------------WMTETGgIMIANYPAMDIKPGSMGKPLPGIEAAIVDDQ------------- 393
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 412 GNETEtrvtPGfeeTEGELLVRG--PSVFRGYWDKPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYK 488
Cdd:PRK04319 394 GNELP----PN---RMGNLAIKKgwPSMMRGIWNNPEKYESYF-AGDWYVSGDSAYMdEDGYFWFQGRVD-DVIKTSGER 464
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 489 VSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSH---RELKEWARGVLAPYAVPSELLLVEEIPRNQ 565
Cdd:PRK04319 465 VGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYEPSEelkEEIRGFVKKGLGAHAAPREIEFKDKLPKTR 544
|
....*....
gi 743747175 566 MGKVNKRDL 574
Cdd:PRK04319 545 SGKIMRRVL 553
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
35-571 |
2.10e-47 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 174.18 E-value: 2.10e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 35 GPAARGNRSAP-VFTR-ALAFGDRVALADqhgshipAGSH-TYRDLYSRSlslAREICRLRACsdGDLREERVSFLCSND 111
Cdd:PRK06155 14 DPLPPSERTLPaMLARqAERYPDRPLLVF-------GGTRwTYAEAARAA---AAAAHALAAA--GVKRGDRVALMCGNR 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 112 VSYV-VAQWASWMsGGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPV---------VRKLGVPLLPLPPTVY 181
Cdd:PRK06155 82 IEFLdVFLGCAWL-GAIAVPINTALRGPQLEHILRNSGARLLVVEAALLAALEAAdpgdlplpaVWLLDAPASVSVPAGW 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 182 CGAAKDPGEEQVPERDWR--DRGAmIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVvNK 259
Cdd:PRK06155 161 STAPLPPLDAPAPAAAVQpgDTAA-ILYTSGTTGPSKGVCCPHAQFYWWGRNSAEDLEIGADDVLYTTLPLFHTNAL-NA 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 260 LLCPLWVGATCVMLPEFSAQLVWEKFLSSQSPRVNVFMA-VPTIYSKLMDYHDR-HfgqphvrdfvravceekiRLMVSG 337
Cdd:PRK06155 239 FFQALLAGATYVLEPRFSASGFWPAVRRHGATVTYLLGAmVSILLSQPARESDRaH------------------RVRVAL 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 338 SAALPLPVLEKWKGATGHTLLERYGMTEIGMALSNPLATARlPGSVGTPLPGVEVRIVSENPQKdgcpyiihaegnetet 417
Cdd:PRK06155 301 GPGVPAALHAAFRERFGVDLLDGYGSTETNFVIAVTHGSQR-PGSMGRLAPGFEARVVDEHDQE---------------- 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 418 rVTPGfeeTEGELLVRG--PSVF-RGYWDKPEETKNAFtSDGWFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALE 493
Cdd:PRK06155 364 -LPDG---EPGELLLRAdePFAFaTGYFGMPEKTVEAW-RNLWFHTGDRVVRDaDGWFRFVDRIK-DAIRRRGENISSFE 437
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 743747175 494 VERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNK 571
Cdd:PRK06155 438 VEQVLLSHPAVAAAAVFPVPSELGEDEVMAAVVLRDGTALEPVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQK 515
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
49-574 |
2.28e-47 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 178.90 E-value: 2.28e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALADQHGSHipagshTYRDLYSRSLSLAReicRLRACSDGdlREERVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:COG1020 485 QAARTPDAVAVVFGDQSL------TYAELNARANRLAH---HLRALGVG--PGDLVGVCLERSLEMVVALLAVLKAGAAY 553
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPV-VRKLGVPLLPlpptvycgAAKDPGEEQVPERDwRDRGAMIIY 207
Cdd:COG1020 554 VPLDPAYPAERLAYMLEDAGARLVLTQSALAARLPELgVPVLALDALA--------LAAEPATNPPVPVT-PDDLAYVIY 624
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 208 TSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNkLLCPLWVGATCVMLPEFSAQ--LVWEKF 285
Cdd:COG1020 625 TSGSTGRPKGVMVEHRALVNLLAWMQRRYGLGPGDRVLQFASLSFDASVWE-IFGALLSGATLVLAPPEARRdpAALAEL 703
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 286 LSSQspRVNVFMAVPTIYSKLMDYHDRHFgqPHVRdfvravceekiRLMVSGSaALPLPVLEKWKGATGHT-LLERYGMT 364
Cdd:COG1020 704 LARH--RVTVLNLTPSLLRALLDAAPEAL--PSLR-----------LVLVGGE-ALPPELVRRWRARLPGArLVNLYGPT 767
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 365 E--IGMALSNPLATARLPGSV--GTPLPGVEVRIVSE--NPQKDGCPyiihaegnetetrvtpgfeeteGELLVRGPSVF 438
Cdd:COG1020 768 EttVDSTYYEVTPPDADGGSVpiGRPIANTRVYVLDAhlQPVPVGVP----------------------GELYIGGAGLA 825
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 439 RGYWDKPEETK-----NAFTSDG--WFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVI 510
Cdd:COG1020 826 RGYLNRPELTAerfvaDPFGFPGarLYRTGDLARWlPDGNLEFLGRAD-DQVKIRGFRIELGEIEAALLQHPGVREAVVV 904
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 743747175 511 GVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:COG1020 905 AREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLPLPLTGNGKLDRLAL 968
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
73-574 |
2.91e-47 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 174.18 E-value: 2.91e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLRACSDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTAV---PLYRKHpqaELEYFIQDS-- 147
Cdd:PRK05677 51 TYGELYKLSGAFAAWLQQHTDLKPGD----RIAVQLPNVLQYPVAVFGAMRAGLIVVntnPLYTAR---EMEHQFNDSga 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 148 RSSVVLA-------------GQEHV------ELLSPVVRKL---------------GVPLLPLPPTVYCGAAKDPGEEQV 193
Cdd:PRK05677 124 KALVCLAnmahlaekvlpktGVKHVivtevaDMLPPLKRLLinavvkhvkkmvpayHLPQAVKFNDALAKGAGQPVTEAN 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 194 PERDwrdRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDD---VILHVLPLHHVHGVVNKLLCPLWVGATC 270
Cdd:PRK05677 204 PQAD---DVAVLQYTGGTTGVAKGAMLTHRNLVANMLQCRALMGSNLNEgceILIAPLPLYHIYAFTFHCMAMMLIGNHN 280
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 271 VM------LPEFSAQLVWEKFLSsqsprvnvFMAVPTIYSKLMdyhdrhfgqpHVRDFvRAVCEEKIRLMVSGSAALPLP 344
Cdd:PRK05677 281 ILisnprdLPAMVKELGKWKFSG--------FVGLNTLFVALC----------NNEAF-RKLDFSALKLTLSGGMALQLA 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 345 VLEKWKGATGHTLLERYGMTEIG-MALSNPLATARLpGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGNETEtrvtpgF 423
Cdd:PRK05677 342 TAERWKEVTGCAICEGYGMTETSpVVSVNPSQAIQV-GTIGIPVPSTLCKVIDD-------------DGNELP------L 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 424 EETeGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHP 502
Cdd:PRK05677 402 GEV-GELCVKGPQVMKGYWQRPEATDEILDSDGWLKTGDIALIQeDGYMRIVDRKK-DMILVSGFNVYPNELEDVLAALP 479
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 743747175 503 SIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK05677 480 GVLQCAAIGVPDEKSGEAIKVFVVVKPGETLTKEQVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRREL 551
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
55-574 |
3.33e-47 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 172.53 E-value: 3.33e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADQHGSHipagshTYRDLYSRSLSLAReicRLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRK 134
Cdd:cd17651 10 DAPALVAEGRRL------TYAELDRRANRLAH---RLRA--RGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPA 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 135 HPQAELEYFIQDSRSSVVLAgQEHVELLSPVVRKLGVPLLPLpptvycGAAKDPGEEQVPERDwRDRGAMIIYTSGTTGR 214
Cdd:cd17651 79 YPAERLAFMLADAGPVLVLT-HPALAGELAVELVAVTLLDQP------GAAAGADAEPDPALD-ADDLAYVIYTSGSTGR 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 215 PKGVVITHHNV-----WATVTGLVH------KWAWTKDDVILHvlplhhvhgvvnKLLCPLWVGATCVMLPE-----FSA 278
Cdd:cd17651 151 PKGVVMPHRSLanlvaWQARASSLGpgartlQFAGLGFDVSVQ------------EIFSTLCAGATLVLPPEevrtdPPA 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 QLVWekfLSSQspRVN-VFMavPTIYSKLMDYHDRHFGQPH--VRDFV----RAVCEEKIRLMVsgsAALPlpvlekwkg 351
Cdd:cd17651 219 LAAW---LDEQ--RISrVFL--PTVALRALAEHGRPLGVRLaaLRYLLtggeQLVLTEDLREFC---AGLP--------- 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 352 atGHTLLERYGMTE----IGMALSNPLATARLPGSVGTPLPGVEVRIVSENPQKdgcpyiihaegnetetrVTPGfeeTE 427
Cdd:cd17651 280 --GLRLHNHYGPTEthvvTALSLPGDPAAWPAPPPIGRPIDNTRVYVLDAALRP-----------------VPPG---VP 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 428 GELLVRGPSVFRGYWDKPEETKNAFTSDGW------FKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLG 500
Cdd:cd17651 338 GELYIGGAGLARGYLNRPELTAERFVPDPFvpgarmYRTGDLARWlPDGELEFLGRAD-DQVKIRGFRIELGEIEAALAR 416
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 743747175 501 HPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17651 417 HPGVREAVVLAREDRPGEKRLVAYVVGDPEAPVDAAELRAALATHLPEYMVPSAFVLLDALPLTPNGKLDRRAL 490
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
71-574 |
3.36e-47 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 172.78 E-value: 3.36e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 71 SHTYRDLYSRSLSLAReicRLRACSdgdLRE-ERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRS 149
Cdd:PRK08276 11 VVTYGELEARSNRLAH---GLRALG---LREgDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGA 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 150 SVVLAGQEhvelLSPVVRKLGVPLLPLPPTVYCGAAKDPGEE-------QVPERDWRDR--GAMIIYTSGTTGRPKGVV- 219
Cdd:PRK08276 85 KVLIVSAA----LADTAAELAAELPAGVPLLLVVAGPVPGFRsyeealaAQPDTPIADEtaGADMLYSSGTTGRPKGIKr 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 220 -ITH---HNVWATVTGLVHKWAWTKDD-VILHVLPLHHV-----HGVVNKLlcplwvGATCVMLPEFSAqlvwEKFLSS- 288
Cdd:PRK08276 161 pLPGldpDEAPGMMLALLGFGMYGGPDsVYLSPAPLYHTaplrfGMSALAL------GGTVVVMEKFDA----EEALALi 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 289 QSPRVNVFMAVPTIYSKLMdyhdrhfgqpHVRDFVRAVCE-EKIRLMVSGSAALPLPV----LEKWkgatGHTLLERYGM 363
Cdd:PRK08276 231 ERYRVTHSQLVPTMFVRML----------KLPEEVRARYDvSSLRVAIHAAAPCPVEVkramIDWW----GPIIHEYYAS 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 364 TE-IGMALSNPLATARLPGSVGTPLPGvEVRIVSENpqkdgcpyiihaeGNEtetrVTPGfeeTEGELLVRGPSVFRGYW 442
Cdd:PRK08276 297 SEgGGVTVITSEDWLAHPGSVGKAVLG-EVRILDED-------------GNE----LPPG---EIGTVYFEMDGYPFEYH 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 443 DKPEETKNAFTSDGWFKTGDTA-VFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRV 521
Cdd:PRK08276 356 NDPEKTAAARNPHGWVTVGDVGyLDEDGYLYLTDRKS-DMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERV 434
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 522 TAVVTLREGHSLS---HRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK08276 435 KAVVQPADGADAGdalAAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRL 490
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
26-576 |
6.64e-47 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 172.71 E-value: 6.64e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 26 PGGRSPLHTGPAarGNRSAPVFTRALAFGDRVALADQHGSHipagSHTYRDLYSRSLSLAREICRLracsdgDLREERVS 105
Cdd:cd17642 5 PGPFYPLEDGTA--GEQLHKAMKRYASVPGTIAFTDAHTGV----NYSYAEYLEMSVRLAEALKKY------GLKQNDRI 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 106 FLCS-NDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKL--------------- 169
Cdd:cd17642 73 AVCSeNSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVFCSKKGLQKVLNVQKKLkiiktiiildskedy 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 170 -GVPLLPLPPTVYCGAAKDPGEEQVPERDWRDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGL---VHKWAWTKDDVIL 245
Cdd:cd17642 153 kGYQCLYTFITQNLPPGFNEYDFKPPSFDRDEQVALIMNSSGSTGLPKGVQLTHKNIVARFSHArdpIFGNQIIPDTAIL 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 246 HVLPLHHVHGVVNkLLCPLWVGATCVMLPEFSAQLvwekFLSS-QSPRVNVFMAVPTIY-----SKLMDYHDRHfgqphv 319
Cdd:cd17642 233 TVIPFHHGFGMFT-TLGYLICGFRVVLMYKFEEEL----FLRSlQDYKVQSALLVPTLFaffakSTLVDKYDLS------ 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 320 rdfvravceekiRLMVSGSAALPLpvlekwKGATGHTLLER---------YGMTEIGMA-LSNPLATARlPGSVGTPLPG 389
Cdd:cd17642 302 ------------NLHEIASGGAPL------SKEVGEAVAKRfklpgirqgYGLTETTSAiLITPEGDDK-PGAVGKVVPF 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 390 VEVRIVsenpqkdgcpyiihaegnETETRVTPGFEETeGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KD 468
Cdd:cd17642 363 FYAKVV------------------DLDTGKTLGPNER-GELCVKGPMIMKGYVNNPEATKALIDKDGWLHSGDIAYYdED 423
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 469 GSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWargvLAP 548
Cdd:cd17642 424 GHFFIVDRLK-SLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVVVLEAGKTMTEKEVMDY----VAS 498
|
570 580 590
....*....|....*....|....*....|...
gi 743747175 549 YAVPSELL-----LVEEIPRNQMGKVNKRDLVQ 576
Cdd:cd17642 499 QVSTAKRLrggvkFVDEVPKGLTGKIDRRKIRE 531
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
203-574 |
7.48e-47 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 172.90 E-value: 7.48e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATV--TGLVHKWAWTKDD-----VILHVLPLHHVHGV-VNKLLcPLWVGATCVMLP 274
Cdd:PRK07059 207 AFLQYTGGTTGVSKGATLLHRNIVANVlqMEAWLQPAFEKKPrpdqlNFVCALPLYHIFALtVCGLL-GMRTGGRNILIP 285
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 275 E------FSAQLvwEKFlssqspRVNVFMAVPTIYSKLMDYHDrhFGQphvRDFvravceEKIRLMVSGSAALPLPVLEK 348
Cdd:PRK07059 286 NprdipgFIKEL--KKY------QVHIFPAVNTLYNALLNNPD--FDK---LDF------SKLIVANGGGMAVQRPVAER 346
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 349 WKGATGHTLLERYGMTEIG-MALSNPLATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGNEtetrVTPGfeeTE 427
Cdd:PRK07059 347 WLEMTGCPITEGYGLSETSpVATCNPVDATEFSGTIGLPLPSTEVSIRDD-------------DGND----LPLG---EP 406
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 428 GELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFKDgsywiRGRTSV-----DIIKTGGYKVSALEVERLLLGHP 502
Cdd:PRK07059 407 GEICIRGPQVMAGYWNRPDETAKVMTADGFFRTGDVGVMDE-----RGYTKIvdrkkDMILVSGFNVYPNEIEEVVASHP 481
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 743747175 503 SIADVAVIGVPDVTWGQRVTAVVtLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK07059 482 GVLEVAAVGVPDEHSGEAVKLFV-VKKDPALTEEDVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRREL 552
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
60-569 |
1.93e-46 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 170.20 E-value: 1.93e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 60 ADQHGshipaGSHTYRDLYSRSLSLAREIcrlracSDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAE 139
Cdd:cd05909 1 EDTLG-----TSLTYRKLLTGAIALARKL------AKMTKEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRE 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 140 LEYFIQDSRSSVVLAGQEHVELLSpvvRKLGVPLLPLPPTVY-------------CGAAKdpgEEQVPERDWR------- 199
Cdd:cd05909 70 LRACIKLAGIKTVLTSKQFIEKLK---LHHLFDVEYDARIVYledlrakiskadkCKAFL---AGKFPPKWLLrifgvap 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 200 ---DRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLP-- 274
Cdd:cd05909 144 vqpDDPAVILFTSGSEGLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKVVFHPnp 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 275 ---EFSAQLVWEKflssqspRVNVFMAVPTIYsklmdyhdrhfgqphvRDFVRAVCEE---KIRLMVSGSAALPLPVLEK 348
Cdd:cd05909 224 ldyKKIPELIYDK-------KATILLGTPTFL----------------RGYARAAHPEdfsSLRLVVAGAEKLKDTLRQE 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 349 WKGATGHTLLERYGMTEIGMALS-NPLATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaegnETETRVTpgfEETE 427
Cdd:cd05909 281 FQEKFGIRILEGYGTTECSPVISvNTPQSPNKEGTVGRPLPGMEVKIVSV----------------ETHEEVP---IGEG 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 428 GELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGH-PSIA 505
Cdd:cd05909 342 GLLLVRGPNVMLGYLNEPELTSFAF-GDGWYDTGDIGKIdGEGFLTITGRLS-RFAKIAGEMVSLEAIEDILSEIlPEDN 419
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 743747175 506 DVAVIGVPDVTWGQRVTAVVTlreGHSLSHRELKEWARGVLAP-YAVPSELLLVEEIPRNQMGKV 569
Cdd:cd05909 420 EVAVVSVPDGRKGEKIVLLTT---TTDTDPSSLNDILKNAGISnLAKPSYIHQVEEIPLLGTGKP 481
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
49-574 |
8.18e-46 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 168.61 E-value: 8.18e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALADQhgshipAGSHTYRDLYSRSLSLAReicRLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:cd17646 7 QAARTPDAPAVVDE------GRTLTYRELDERANRLAH---LLRA--RGVGPEDRVAVLLPRSADLVVALLAVLKAGAAY 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPLLPLpptvycgAAKDPGEEQVPERdwRDRGAMIIYT 208
Cdd:cd17646 76 LPLDPGYPADRLAYMLADAGPAVVLTTADLAARLPAGGDVALLGDEAL-------AAPPATPPLVPPR--PDNLAYVIYT 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 209 SGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLhhvhGV---VNKLLCPLWVGATCVML-------PEFSA 278
Cdd:cd17646 147 SGSTGRPKGVMVTHAGIVNRLLWMQDEYPLGPGDRVLQKTPL----SFdvsVWELFWPLVAGARLVVArpgghrdPAYLA 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 QLVWEKflssqspRVNVFMAVPTIysklmdyhdrhfgqphVRDFVRAVCEEK---IRLMVSGSAALPLPVLEKWKGATGH 355
Cdd:cd17646 223 ALIREH-------GVTTCHFVPSM----------------LRVFLAEPAAGScasLRRVFCSGEALPPELAARFLALPGA 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 356 TLLERYGMTEIGMALSNPLATARLPG---SVGTPLPGVEVrivsenpqkdgcpYIIHAEGNetetRVTPGfeeTEGELLV 432
Cdd:cd17646 280 ELHNLYGPTEAAIDVTHWPVRGPAETpsvPIGRPVPNTRL-------------YVLDDALR----PVPVG---VPGELYL 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 433 RGPSVFRGYWDKPEETKNAFTSDgWF-------KTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSI 504
Cdd:cd17646 340 GGVQLARGYLGRPALTAERFVPD-PFgpgsrmyRTGDLARWRpDGALEFLGRSD-DQVKIRGFRVEPGEIEAALAAHPAV 417
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 743747175 505 ADVAVIGVPDVTWGQRVTAVVTLREGHS-LSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17646 418 THAVVVARAAPAGAARLVGYVVPAAGAAgPDTAALRAHLAERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
50-582 |
2.05e-45 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 168.59 E-value: 2.05e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 50 ALAFGDRVALAdqHGSHipagSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAV 129
Cdd:PRK08162 28 AEVYPDRPAVI--HGDR----RRTWAETYARCRRLASALARR-----GIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLN 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 130 PLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPLLPLPPTV---YCG-------------AAKDPG-EEQ 192
Cdd:PRK08162 97 TLNTRLDAASIAFMLRHGEAKVLIVDTEFAEVAREALALLPGPKPLVIDVDdpeYPGgrfigaldyeaflASGDPDfAWT 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 193 VPERDWRdrgAMII-YTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGvvnklLCPLW----VG 267
Cdd:PRK08162 177 LPADEWD---AIALnYTSGTTGNPKGVVYHHRGAYLNALSNILAWGMPKHPVYLWTLPMFHCNG-----WCFPWtvaaRA 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 268 ATCVMLPEFSAQLVWEKFlssQSPRVNVFMAVPTIYSKLMdyhdrhfgqpHVRDFVRAVCEEKIRLMVSGsAALPLPVLE 347
Cdd:PRK08162 249 GTNVCLRKVDPKLIFDLI---REHGVTHYCGAPIVLSALI----------NAPAEWRAGIDHPVHAMVAG-AAPPAAVIA 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 348 KWKgATGHTLLERYGMTEI-GmalsnplatarlPGSVGTPLPGVEVRIVSENPQKD---GCPYIIHAEGN----ETETRV 419
Cdd:PRK08162 315 KME-EIGFDLTHVYGLTETyG------------PATVCAWQPEWDALPLDERAQLKarqGVRYPLQEGVTvldpDTMQPV 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 420 tPGFEETEGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRtSVDIIKTGGYKVSALEVERLL 498
Cdd:PRK08162 382 -PADGETIGEIMFRGNIVMKGYLKNPKATEEAF-AGGWFHTGDLAVLhPDGYIKIKDR-SKDIIISGGENISSIEVEDVL 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 499 LGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLvEEIPRNQMGKVNK---RDLV 575
Cdd:PRK08162 459 YRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASATEEEIIAHCREHLAGFKVPKAVVF-GELPKTSTGKIQKfvlREQA 537
|
....*..
gi 743747175 576 QQLYPQD 582
Cdd:PRK08162 538 KSLKAID 544
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
49-579 |
3.19e-44 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 165.10 E-value: 3.19e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALADQHGSHIPAGSHTYRDLYSRSLSLAREIcrLRACSDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:cd05931 2 RAAARPDRPAYTFLDDEGGREETLTYAELDRRARAIAARL--QAVGKPGD----RVLLLAPPGLDFVAAFLGCLYAGAIA 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLYRKHPQAELEYF---IQDSRSSVVLAGQEHVELLSPVVRKLGVPLLPLPPTVYCGAAKDPGEEQVPERDwRDRGAMI 205
Cdd:cd05931 76 VPLPPPTPGRHAERLaaiLADAGPRVVLTTAAALAAVRAFAASRPAAGTPRLLVVDLLPDTSAADWPPPSPD-PDDIAYL 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 206 IYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLP--EFSAQ-LVW 282
Cdd:cd05931 155 QYTSGSTGTPKGVVVTHRNLLANVRQIRRAYGLDPGDVVVSWLPLYHDMGLIGGLLTPLYSGGPSVLMSpaAFLRRpLRW 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 283 EKFLSSqspRVNVFMAVPTiysklmdyhdrhFGQPHVrdfVRAVCEEKI------RLMVSGSAALPL--PVLEKWK---- 350
Cdd:cd05931 235 LRLISR---YRATISAAPN------------FAYDLC---VRRVRDEDLegldlsSWRVALNGAEPVrpATLRRFAeafa 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 351 --GATGHTLLERYGMTEIGMALSNP----------LATARLPG----------------SVGTPLPGVEVRIVseNPqkd 402
Cdd:cd05931 297 pfGFRPEAFRPSYGLAEATLFVSGGppgtgpvvlrVDRDALAGravavaaddpaarelvSCGRPLPDQEVRIV--DP--- 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 403 gcpyiihaegnETETRVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAF------TSDGWFKTGDTAVFKDGSYWIRGR 476
Cdd:cd05931 372 -----------ETGRELPDG---EVGEIWVRGPSVASGYWGRPEATAETFgalaatDEGGWLRTGDLGFLHDGELYITGR 437
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 477 TSvDIIKTGGYKVSALEVERLLLGHPS---IADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARG-VLAPYAV- 551
Cdd:cd05931 438 LK-DLIIVRGRNHYPQDIEATAEEAHPalrPGCVAAFSVPDDGEERLVVVAEVERGADPADLAAIAAAIRAaVAREHGVa 516
|
570 580 590
....*....|....*....|....*....|
gi 743747175 552 PSELLLVE--EIPRNQMGKVnKRDLVQQLY 579
Cdd:cd05931 517 PADVVLVRpgSIPRTSSGKI-QRRACRAAY 545
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
203-574 |
1.08e-43 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 163.89 E-value: 1.08e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGlVHKWAWTKD------DVILHVLPLHHVHGVVNKLLCPLWVGAtCVML--- 273
Cdd:PRK08751 211 AFLQYTGGTTGVAKGAMLTHRNLVANMQQ-AHQWLAGTGkleegcEVVITALPLYHIFALTANGLVFMKIGG-CNHLisn 288
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 274 ----PEFSAQLvwekflssQSPRVNVFMAVPTIYSKLMDYHDrhFGQphvRDFvravceEKIRLMVSGSAALPLPVLEKW 349
Cdd:PRK08751 289 prdmPGFVKEL--------KKTRFTAFTGVNTLFNGLLNTPG--FDQ---IDF------SSLKMTLGGGMAVQRSVAERW 349
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 350 KGATGHTLLERYGMTEIG-MALSNPLATARLPGSVGTPLPGVEVRIVSENPQkdgcpyiIHAEGNEtetrvtpgfeeteG 428
Cdd:PRK08751 350 KQVTGLTLVEAYGLTETSpAACINPLTLKEYNGSIGLPIPSTDACIKDDAGT-------VLAIGEI-------------G 409
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 429 ELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADV 507
Cdd:PRK08751 410 ELCIKGPQVMKGYWKRPEETAKVMDADGWLHTGDIARMdEQGFVYIVDRKK-DMILVSGFNVYPNEIEDVIAMMPGVLEV 488
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 743747175 508 AVIGVPDVTWGQrVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK08751 489 AAVGVPDEKSGE-IVKVVIVKKDPALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRREL 554
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
73-511 |
1.09e-43 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 162.14 E-value: 1.09e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAreiCRLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:cd17640 7 TYKDLYQEILDFA---AGLRSL--GVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVAL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAgqehvellspvvrklgvpllplpptvycgaakDPGEEQVperdwrdrgAMIIYTSGTTGRPKGVVITHHNVwatVTGL 232
Cdd:cd17640 82 VV--------------------------------ENDSDDL---------ATIIYTSGTTGNPKGVMLTHANL---LHQI 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 233 VHKWAWTK---DDVILHVLPLHHVHGVVNKLLCPLWvGATCVmlpeFSAQLVWEKFLSSQSPrvNVFMAVP----TIYSK 305
Cdd:cd17640 118 RSLSDIVPpqpGDRFLSILPIWHSYERSAEYFIFAC-GCSQA----YTSIRTLKDDLKRVKP--HYIVSVPrlweSLYSG 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 306 LmdyHDRHFGQPHVRDFV--RAVCEEKIRLMVSGSAALPlPVLEKWKGATGHTLLERYGMTEIGmalsnPLATARLP--- 380
Cdd:cd17640 191 I---QKQVSKSSPIKQFLflFFLSGGIFKFGISGGGALP-PHVDTFFEAIGIEVLNGYGLTETS-----PVVSARRLkcn 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 381 --GSVGTPLPGVEVRIVSENpqkdgcpyiihaeGNETetrVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDGWF 458
Cdd:cd17640 262 vrGSVGRPLPGTEIKIVDPE-------------GNVV---LPPG---EKGIVWVRGPQVMKGYYKNPEATSKVLDSDGWF 322
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....
gi 743747175 459 KTGDTAVF-KDGSYWIRGRTSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIG 511
Cdd:cd17640 323 NTGDLGWLtCGGELVLTGRAKDTIVLSNGENVEPQPIEEALMRSPFIEQIMVVG 376
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
96-575 |
5.16e-43 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 160.30 E-value: 5.16e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 96 DGDLREERVSFLCSNDVSYVVAQWASWMSGGT----AVPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRklgv 171
Cdd:cd05922 13 AGGVRGERVVLILPNRFTYIELSFAVAYAGGRlglvFVPLNPTLKESVLRYLVADAGGRIVLADAGAADRLRDALP---- 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 172 pllplpptvycgAAKDPGEeQVPERDWRDRG-------------AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAW 238
Cdd:cd05922 89 ------------ASPDPGT-VLDADGIRAARasapahevshedlALLLYTSGSTGSPKLVRLSHQNLLANARSIAEYLGI 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 239 TKDDVILHVLPLHHVHGVvNKLLCPLWVGATCVMLPEFS-AQLVWEKFLSSqspRVNVFMAVPTIYSKL--MDYHDRhfG 315
Cdd:cd05922 156 TADDRALTVLPLSYDYGL-SVLNTHLLRGATLVLTNDGVlDDAFWEDLREH---GATGLAGVPSTYAMLtrLGFDPA--K 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 316 QPHVRDFVRAvceekirlmvsgSAALPLPVL----EKWKGATGHTLlerYGMTEI--GMALSNPLATARLPGSVGTPLPG 389
Cdd:cd05922 230 LPSLRYLTQA------------GGRLPQETIarlrELLPGAQVYVM---YGQTEAtrRMTYLPPERILEKPGSIGLAIPG 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 390 VEVRIVsenpqkdgcpyiihaegNETETRVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KD 468
Cdd:cd05922 295 GEFEIL-----------------DDDGTPTPPG---EPGEIVHRGPNVMKGYWNDPPYRRKEGRGGGVLHTGDLARRdED 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 469 GSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDvTWGQRVTAVVTLREGHSLShrELKEWARGVLAP 548
Cdd:cd05922 355 GFLFIVGRRD-RMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPD-PLGEKLALFVTAPDKIDPK--DVLRSLAERLPP 430
|
490 500
....*....|....*....|....*..
gi 743747175 549 YAVPSELLLVEEIPRNQMGKVNKRDLV 575
Cdd:cd05922 431 YKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
203-571 |
1.03e-42 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 156.27 E-value: 1.03e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLV-HKWAWTKDDVILHVLPLHHVHGVVNKLLCpLWVGATCVMLPEFSAQLV 281
Cdd:cd17635 4 LAVIFTSGTTGEPKAVLLANKTFFAVPDILQkEGLNWVVGDVTYLPLPATHIGGLWWILTC-LIHGGLCVTGGENTTYKS 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 282 WEKFLssQSPRVNVFMAVPTIYSKLMDyhdrhfgqpHVRDFVRAVceEKIRLMVSGSAaLPLPVLEKWKGATGHT-LLER 360
Cdd:cd17635 83 LFKIL--TTNAVTTTCLVPTLLSKLVS---------ELKSANATV--PSLRLIGYGGS-RAIAADVRFIEATGLTnTAQV 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 361 YGMTEIGMALSNPLATARLP-GSVGTPLPGVEVrivsenpqkdgcpYIIHAEGNEtetrvtpGFEETEGELLVRGPSVFR 439
Cdd:cd17635 149 YGLSETGTALCLPTDDDSIEiNAVGRPYPGVDV-------------YLAATDGIA-------GPSASFGTIWIKSPANML 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 440 GYWDKPEETKNAFTsDGWFKTGDTA-VFKDGSYWIRGRTSVDIIKtGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWG 518
Cdd:cd17635 209 GYWNNPERTAEVLI-DGWVNTGDLGeRREDGFLFITGRSSESINC-GGVKIAPDEVERIAEGVSGVQECACYEISDEEFG 286
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....
gi 743747175 519 QRVTAVVTLREGHSLSH-RELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNK 571
Cdd:cd17635 287 ELVGLAVVASAELDENAiRALKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
55-574 |
1.58e-42 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 159.36 E-value: 1.58e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADqhgshiPAGSHTYRDLYSRSLSLAReicRLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRK 134
Cdd:cd12114 2 DATAVIC------GDGTLTYGELAERARRVAG---ALKAA--GVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDID 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 135 HPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPllplpptvycGAAKDPGEEQVPERDWRDRGAMIIYTSGTTGR 214
Cdd:cd12114 71 QPAARREAILADAGARLVLTDGPDAQLDVAVFDVLILD----------LDALAAPAPPPPVDVAPDDLAYVIFTSGSTGT 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 215 PKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNkLLCPLWVGATCVMLPEFSAQ--LVWEKFLssQSPR 292
Cdd:cd12114 141 PKGVMISHRAALNTILDINRRFAVGPDDRVLALSSLSFDLSVYD-IFGALSAGATLVLPDEARRRdpAHWAELI--ERHG 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 293 VNVFMAVPTIYSKLMDYhdrhfgqphVRDFVRAVCeeKIRL-MVSG---SAALPLPVLEKWKGATGHTLlerYGMTEiGM 368
Cdd:cd12114 218 VTLWNSVPALLEMLLDV---------LEAAQALLP--SLRLvLLSGdwiPLDLPARLRALAPDARLISL---GGATE-AS 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 369 ALSNPLATARLP---GSV--GTPLPGVEVRIVseNPQKDGCPyiihaegnetetrvtpgfEETEGELLVRGPSVFRGYWD 443
Cdd:cd12114 283 IWSIYHPIDEVPpdwRSIpyGRPLANQRYRVL--DPRGRDCP------------------DWVPGELWIGGRGVALGYLG 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 444 KPEETKNAFTSDG----WFKTGDTAVFK-DGSYWIRGRtsVDI-IKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTW 517
Cdd:cd12114 343 DPELTAARFVTHPdgerLYRTGDLGRYRpDGTLEFLGR--RDGqVKVRGYRIELGEIEAALQAHPGVARAVVVVLGDPGG 420
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 743747175 518 GQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd12114 421 KRLAAFVVPDNDGTPIAPDALRAFLAQTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
204-569 |
2.79e-42 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 154.49 E-value: 2.79e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 204 MIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVvNKLLCPLWVGATCVMLPEFSAQLVWE 283
Cdd:cd17633 4 YIGFTSGTTGLPKAYYRSERSWIESFVCNEDLFNISGEDAILAPGPLSHSLFL-YGAISALYLGGTFIGQRKFNPKSWIR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 KfLSSQSprVNVFMAVPTIYSKLMDyHDRHfgqphvrdfvravcEEKIRLMVSGSAALPLPVLEKWKGATGHT-LLERYG 362
Cdd:cd17633 83 K-INQYN--ATVIYLVPTMLQALAR-TLEP--------------ESKIKSIFSSGQKLFESTKKKLKNIFPKAnLIEFYG 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 363 MTEIGMALSNPLATARLPGSVGTPLPGVEVRIVSenpqkdgcpyiihAEGNETetrvtpgfeeteGELLVRGPSVFRGYW 442
Cdd:cd17633 145 TSELSFITYNFNQESRPPNSVGRPFPNVEIEIRN-------------ADGGEI------------GKIFVKSEMVFSGYV 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 443 DKpeetkNAFTSDGWFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRV 521
Cdd:cd17633 200 RG-----GFSNPDGWMSVGDIGYVDeEGYLYLVGRES-DMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIA 273
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 743747175 522 TAVVTlreGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKV 569
Cdd:cd17633 274 VALYS---GDKLTYKQLKRFLKQKLSRYEIPKKIIFVDSLPYTSSGKI 318
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
71-574 |
3.08e-42 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 160.01 E-value: 3.08e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 71 SHTYRDLYSRSLSLAREICRLRACSDGDLreerVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSS 150
Cdd:PLN02574 66 SISYSELQPLVKSMAAGLYHVMGVRQGDV----VLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVG 141
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 151 VVLAGQEHVELLSPV-VRKLGV-------PLLPLPPTVYCGAAKDPGEEQVPERDWRDRGAmIIYTSGTTGRPKGVVITH 222
Cdd:PLN02574 142 LAFTSPENVEKLSPLgVPVIGVpenydfdSKRIEFPKFYELIKEDFDFVPKPVIKQDDVAA-IMYSSGTTGASKGVVLTH 220
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 223 HNVWATVTGLVHKWAW-----TKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQ---LVWEKFlssqspRVN 294
Cdd:PLN02574 221 RNLIAMVELFVRFEASqyeypGSDNVYLAALPMFHIYGLSLFVVGLLSLGSTIVVMRRFDASdmvKVIDRF------KVT 294
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 295 VFMAVPTIYSKLMdyhdrhfgqpHVRDFVRAVCEEKIRLMVSGSAALPLPVLEKWKGATGHT-LLERYGMTEIGMALSNP 373
Cdd:PLN02574 295 HFPVVPPILMALT----------KKAKGVCGEVLKSLKQVSCGAAPLSGKFIQDFVQTLPHVdFIQGYGMTESTAVGTRG 364
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 374 LATARLP--GSVGTPLPGVEVRIVSEnpqkdgcpyiihaegnETETRVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNA 451
Cdd:PLN02574 365 FNTEKLSkySSVGLLAPNMQAKVVDW----------------STGCLLPPG---NCGELWIQGPGVMKGYLNNPKATQST 425
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 452 FTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREG 530
Cdd:PLN02574 426 IDKDGWLRTGDIAYFdEDGYLYIVDRLK-EIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQG 504
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 743747175 531 HSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PLN02574 505 STLSQEAVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRREL 548
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
55-574 |
9.82e-42 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 158.00 E-value: 9.82e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADQhgshipAGSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRK 134
Cdd:PRK13382 58 DRPGLIDE------LGTLTWRELDERSDALAAALQAL-----PIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTS 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 135 HPQAELEYFIQDSRSSVVLAGQEHVELLS------PVVRKLGVPLLPLPPTVYCGAAKDPGEEQVPERDwrDRGAMIIYT 208
Cdd:PRK13382 127 FAGPALAEVVTREGVDTVIYDEEFSATVDraladcPQATRIVAWTDEDHDLTVEVLIAAHAGQRPEPTG--RKGRVILLT 204
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 209 SGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNkLLCPLWVGATCVML----PEFSAQLVwEK 284
Cdd:PRK13382 205 SGTTGTPKGARRSGPGGIGTLKAILDRTPWRAEEPTVIVAPMFHAWGFSQ-LVLAASLACTIVTRrrfdPEATLDLI-DR 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 285 FlssqspRVNVFMAVPTIYSKLMDYHDRHFGQPHVRdfvravceeKIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMT 364
Cdd:PRK13382 283 H------RATGLAVVPVMFDRIMDLPAEVRNRYSGR---------SLRFAAASGSRMRPDVVIAFMDQFGDVIYNNYNAT 347
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 365 EIGM-ALSNPLATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGNEtetrVTPGfeETeGELLVRGPSVFRGYwd 443
Cdd:PRK13382 348 EAGMiATATPADLRAAPDTAGRPAEGTEIRILDQ-------------DFRE----VPTG--EV-GTIFVRNDTQFDGY-- 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 444 KPEETKNafTSDGWFKTGDTAVFKD-GSYWIRGRtSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVT 522
Cdd:PRK13382 406 TSGSTKD--FHDGFMASGDVGYLDEnGRLFVVGR-DDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLA 482
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 743747175 523 AVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK13382 483 AFVVLKPGASATPETLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRREL 534
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
50-574 |
1.05e-41 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 156.37 E-value: 1.05e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 50 ALAFGDRvaladqhgshipagSHTYRDLYSRSLSLAReicRLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAV 129
Cdd:cd17649 5 ALVFGDQ--------------SLSYAELDARANRLAH---RLRAL--GVGPEVRVGIALERSLEMVVALLAILKAGGAYV 65
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 130 PLYRKHPQAELEYFIQDSRSSVVLAgqEHVELLspvvrklgvpllplpptvycgaakdpgeeqvperdwrdrgAMIIYTS 209
Cdd:cd17649 66 PLDPEYPAERLRYMLEDSGAGLLLT--HHPRQL----------------------------------------AYVIYTS 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 210 GTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLhHVHGVVNKLLCPLWVGATCVMLPEfsAQLVWEKFLSSQ 289
Cdd:cd17649 104 GSTGTPKGVAVSHGPLAAHCQATAERYGLTPGDRELQFASF-NFDGAHEQLLPPLICGACVVLRPD--ELWASADELAEM 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 290 --SPRVNVfMAVPTIY-SKLMDYHDRHFGQPHVRdfvravceekIRLMVSGSAALPLPVLEKWkGATGHTLLERYGMTE- 365
Cdd:cd17649 181 vrELGVTV-LDLPPAYlQQLAEEADRTGDGRPPS----------LRLYIFGGEALSPELLRRW-LKAPVRLFNAYGPTEa 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 366 --IGMALSNPLATARLPGSV--GTPLPGVEVRIVSE--NPQKDGCPyiihaegnetetrvtpgfeeteGELLVRGPSVFR 439
Cdd:cd17649 249 tvTPLVWKCEAGAARAGASMpiGRPLGGRSAYILDAdlNPVPVGVT----------------------GELYIGGEGLAR 306
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 440 GYWDKPEETKNAFTSDG-------WFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIG 511
Cdd:cd17649 307 GYLGRPELTAERFVPDPfgapgsrLYRTGDLARWRdDGVIEYLGRVD-HQVKIRGFRIELGEIEAALLEHPGVREAAVVA 385
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 743747175 512 VPDVTwGQRVTAVVTLREGHSLSH--RELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17649 386 LDGAG-GKQLVAYVVLRAAAAQPElrAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKAL 449
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
204-563 |
2.82e-41 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 152.07 E-value: 2.82e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 204 MIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVhGVVNKLLCPLWVGATCVMLPEFSAQLVWE 283
Cdd:cd17636 4 LAIYTAAFSGRPNGALLSHQALLAQALVLAVLQAIDEGTVFLNSGPLFHI-GTLMFTLATFHAGGTNVFVRRVDAEEVLE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 kfLSSQSPRVNVFMAVPTIYSKLMDYHDRHFGQPHVRDFVRAvceekiRLMVSGSAALPLPVLEKWKGatghtllerYGM 363
Cdd:cd17636 83 --LIEAERCTHAFLLPPTIDQIVELNADGLYDLSSLRSSPAA------PEWNDMATVDTSPWGRKPGG---------YGQ 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 364 TEI-GMALSNPLATARLpGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGNEtetrVTPGfeETeGELLVRGPSVFRGYW 442
Cdd:cd17636 146 TEVmGLATFAALGGGAI-GGAGRPSPLVQVRILDE-------------DGRE----VPDG--EV-GEIVARGPTVMAGYW 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 443 DKPEEtkNAF-TSDGWFKTGDTAVFK-DGSYWIRGrTSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQR 520
Cdd:cd17636 205 NRPEV--NARrTRGGWHHTNDLGRREpDGSLSFVG-PKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQS 281
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 743747175 521 VTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPR 563
Cdd:cd17636 282 VKAIVVLKPGASVTEAELIEHCRARIASYKKPKSVEFADALPR 324
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
200-570 |
6.26e-41 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 159.32 E-value: 6.26e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 200 DRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLP----- 274
Cdd:PRK08633 782 DDTATIIFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVILSSLPFFHSFGLTVTLWLPLLEGIKVVYHPdptda 861
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 275 EFSAQLVwEKFlssqspRVNVFMAVPT---IYSKLMDYHDRHFgqphvrdfvravceEKIRLMVSGSAALPLPVLEKWKG 351
Cdd:PRK08633 862 LGIAKLV-AKH------RATILLGTPTflrLYLRNKKLHPLMF--------------ASLRLVVAGAEKLKPEVADAFEE 920
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 352 ATGHTLLERYGMTEigmaLSnPLATARLP---------------GSVGTPLPGVEVRIVseNPqkdgcpyiihaegnETE 416
Cdd:PRK08633 921 KFGIRILEGYGATE----TS-PVASVNLPdvlaadfkrqtgskeGSVGMPLPGVAVRIV--DP--------------ETF 979
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 417 TRVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFT---SDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSAL 492
Cdd:PRK08633 980 EELPPG---EDGLILIGGPQVMKGYLGDPEKTAEVIKdidGIGWYVTGDKGHLdEDGFLTITDRYS-RFAKIGGEMVPLG 1055
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 493 EVERLL---LGHPSIaDVAVIGVPDVTWGQRVTAVVTLREGHSLS-HRELKEwarGVLAPYAVPSELLLVEEIPRNQMGK 568
Cdd:PRK08633 1056 AVEEELakaLGGEEV-VFAVTAVPDEKKGEKLVVLHTCGAEDVEElKRAIKE---SGLPNLWKPSRYFKVEALPLLGSGK 1131
|
..
gi 743747175 569 VN 570
Cdd:PRK08633 1132 LD 1133
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
73-574 |
1.52e-40 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 154.02 E-value: 1.52e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREicrLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:cd17655 24 TYRELNERANQLART---LRE--KGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPDYPEERIQYILEDSGADIL 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAgQEHVELLSpvvRKLGVPLLPLPPTVYCGAAkdpgeEQVPERDWRDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGL 232
Cdd:cd17655 99 LT-QSHLQPPI---AFIGLIDLLDEDTIYHEES-----ENLEPVSKSDDLAYVIYTSGSTGKPKGVMIEHRGVVNLVEWA 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 233 VHKWAWTKDDVILHVLPLhHVHGVVNKLLCPLWVGATCVMLPEfSAQLVWEKFLSSQSP-RVNVFMAVPTiYSKLMDYhd 311
Cdd:cd17655 170 NKVIYQGEHLRVALFASI-SFDASVTEIFASLLSGNTLYIVRK-ETVLDGQALTQYIRQnRITIIDLTPA-HLKLLDA-- 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 312 rhfgqphvrdfVRAVCEEKIRLMVSGSAALPLPVLEKWKGATGH--TLLERYGMTE------IGMALSNPLATARLPgsV 383
Cdd:cd17655 245 -----------ADDSEGLSLKHLIVGGEALSTELAKKIIELFGTnpTITNAYGPTEttvdasIYQYEPETDQQVSVP--I 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 384 GTPLPGVEVRIVSEN--PQKDGCPyiihaegnetetrvtpgfeeteGELLVRGPSVFRGYWDKPEETKNAFTSDGW---- 457
Cdd:cd17655 312 GKPLGNTRIYILDQYgrPQPVGVA----------------------GELYIGGEGVARGYLNRPELTAEKFVDDPFvpge 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 458 --FKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREghSLS 534
Cdd:cd17655 370 rmYRTGDLARWlPDGNIEFLGRID-HQVKIRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIVSEK--ELP 446
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 743747175 535 HRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17655 447 VAQLREFLARELPDYMIPSYFIKLDEIPLTPNGKVDRKAL 486
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
205-572 |
1.66e-40 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 151.00 E-value: 1.66e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITHHNVWATVTG--------------LVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATC 270
Cdd:cd05924 8 ILYTGGTTGMPKGVMWRQEDIFRMLMGgadfgtgeftpsedAHKAAAAAAGTVMFPAPPLMHGTGSWTAFGGLLGGQTVV 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 271 VMLPEFSAQLVWEkflSSQSPRVNVFMAVPTIYSK-LMDYHDRhfgqPHVRDFvravceEKIRLMVSGSAALPLPVLEKW 349
Cdd:cd05924 88 LPDDRFDPEEVWR---TIEKHKVTSMTIVGDAMARpLIDALRD----AGPYDL------SSLFAISSGGALLSPEVKQGL 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 350 KGATGH-TLLERYGMTEIGMalsnplatarlpGSVGTPLPGVEVRIVSENPQKDGCpyIIHAEGNEtetrVTPGfEETEG 428
Cdd:cd05924 155 LELVPNiTLVDAFGSSETGF------------TGSGHSAGSGPETGPFTRANPDTV--VLDDDGRV----VPPG-SGGVG 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 429 ELLVRGpSVFRGYWDKPEETKNAF-TSDG--WFKTGDTA-VFKDGSYWIRGRTSVdIIKTGGYKVSALEVERLLLGHPSI 504
Cdd:cd05924 216 WIARRG-HIPLGYYGDEAKTAETFpEVDGvrYAVPGDRAtVEADGTVTLLGRGSV-CINTGGEKVFPEEVEEALKSHPAV 293
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 743747175 505 ADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKR 572
Cdd:cd05924 294 YDVLVVGRPDERWGQEVVAVVQLREGAGVDLEELREHCRTRIARYKLPKQVVFVDEIERSPAGKADYR 361
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
205-574 |
3.64e-40 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 155.10 E-value: 3.64e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITH--HNVWATVTglvHKWAW-TKDDVIL-----------HVlplHHVHGvvnkllcPLWVGATC 270
Cdd:TIGR02188 242 ILYTSGSTGKPKGVLHTTggYLLYAAMT---MKYVFdIKDGDIFwctadvgwitgHS---YIVYG-------PLANGATT 308
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 271 VML---PEFSAQLVW----EKFlssqspRVNVFMAVPTIYSKLMDYHDRHfgqPHVRDFvravceEKIRLMvsGSAALPL 343
Cdd:TIGR02188 309 VMFegvPTYPDPGRFweiiEKH------KVTIFYTAPTAIRALMRLGDEW---VKKHDL------SSLRLL--GSVGEPI 371
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 344 -PvlEKWK------GATGHTLLERYGMTEIGMALSNPL--ATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGNE 414
Cdd:TIGR02188 372 nP--EAWMwyykvvGKERCPIVDTWWQTETGGIMITPLpgATPTKPGSATLPFFGIEPAVVDE-------------EGNP 436
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 415 TETrvtpgfEETEGELLVRG--PSVFRGYWDKPEETKNAFTSD--GWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKV 489
Cdd:TIGR02188 437 VEG------PGEGGYLVIKQpwPGMLRTIYGDHERFVDTYFSPfpGYYFTGDGARRdKDGYIWITGRVD-DVINVSGHRL 509
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 490 SALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHR---ELKEWARGVLAPYAVPSELLLVEEIPRNQM 566
Cdd:TIGR02188 510 GTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTLKDGYEPDDElrkELRKHVRKEIGPIAKPDKIRFVPGLPKTRS 589
|
....*...
gi 743747175 567 GKVNKRDL 574
Cdd:TIGR02188 590 GKIMRRLL 597
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
49-574 |
4.17e-40 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 152.36 E-value: 4.17e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALadQHGshipAGSHTYRDLYSRSLSLAReicRLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:cd12117 6 QAARTPDAVAV--VYG----DRSLTYAELNERANRLAR---RLRA--AGVGPGDVVGVLAERSPELVVALLAVLKAGAAY 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLYRKHPQAELEYFIQDSRSSVVLAGqehvellspvvRKLGVPLLPLPPTVYCGAAKDPGEEQVPERDWR-DRGAMIIY 207
Cdd:cd12117 75 VPLDPELPAERLAFMLADAGAKVLLTD-----------RSLAGRAGGLEVAVVIDEALDAGPAGNPAVPVSpDDLAYVMY 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 208 TSGTTGRPKGVVITHHNVwatvTGLVHKWAW---TKDDVILHVLPL------HHVHGvvnkllcPLWVGATCVMLP--EF 276
Cdd:cd12117 144 TSGSTGRPKGVAVTHRGV----VRLVKNTNYvtlGPDDRVLQTSPLafdastFEIWG-------ALLNGARLVLAPkgTL 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 277 SAQLVWEKFLSSQspRVNVFMAVPTIYSKLMDYHDRHFGQphvrdfvravceekIRLMVSGSAALPLPVLEKWKGATGH- 355
Cdd:cd12117 213 LDPDALGALIAEE--GVTVLWLTAALFNQLADEDPECFAG--------------LRELLTGGEVVSPPHVRRVLAACPGl 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 356 TLLERYGMTE-IGMALS---NPLATARLPGSVGTPLPGVEVRIVSEnpqkDGCPyiihaegnetetrVTPGfeeTEGELL 431
Cdd:cd12117 277 RLVNGYGPTEnTTFTTShvvTELDEVAGSIPIGRPIANTRVYVLDE----DGRP-------------VPPG---VPGELY 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 432 VRGPSVFRGYWDKPEETKNAFTSDGW------FKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSI 504
Cdd:cd12117 337 VGGDGLALGYLNRPALTAERFVADPFgpgerlYRTGDLARWLpDGRLEFLGRID-DQVKIRGFRIELGEIEAALRAHPGV 415
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 505 ADVAVIGVPDVTWGQRVTAVVTLREGhsLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd12117 416 REAVVVVREDAGGDKRLVAYVVAEGA--LDAAELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
73-574 |
1.23e-39 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 150.10 E-value: 1.23e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAReicRLRACSDGdlREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:cd17652 14 TYAELNARANRLAR---LLAARGVG--PERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYMLADARPALL 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHVellspvvrklgvpllplpptvycgaakdpgeeqvperdwrdrgAMIIYTSGTTGRPKGVVITHHNVWATVTGL 232
Cdd:cd17652 89 LTTPDNL-------------------------------------------AYVIYTSGSTGRPKGVVVTHRGLANLAAAQ 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 233 VHKWAWTKDDVILHVLPLHhVHGVVNKLLCPLWVGATCVMLPEFSAQlvwekflssqsprvnvfmAVPTIYSKLMDYHDR 312
Cdd:cd17652 126 IAAFDVGPGSRVLQFASPS-FDASVWELLMALLAGATLVLAPAEELL------------------PGEPLADLLREHRIT 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 313 HFGQ-PHVRDFVRAVCEEKIRLMVSGSAALPLPVLEKWkgATGHTLLERYGMTE--IGMALSNPLATARLPgSVGTPLPG 389
Cdd:cd17652 187 HVTLpPAALAALPPDDLPDLRTLVVAGEACPAELVDRW--APGRRMINAYGPTEttVCATMAGPLPGGGVP-PIGRPVPG 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 390 VEVRIVSEN--PQKDGCPyiihaegnetetrvtpgfeeteGELLVRGPSVFRGYWDKPEETKNAFTSDGW-------FKT 460
Cdd:cd17652 264 TRVYVLDARlrPVPPGVP----------------------GELYIAGAGLARGYLNRPGLTAERFVADPFgapgsrmYRT 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 461 GDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELK 539
Cdd:cd17652 322 GDLARWRaDGQLEFLGRAD-DQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDDRPGDKRLVAYVVPAPGAAPTAAELR 400
|
490 500 510
....*....|....*....|....*....|....*
gi 743747175 540 EWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17652 401 AHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
37-574 |
1.34e-39 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 151.69 E-value: 1.34e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 37 AARG--NRSAPVFTRALAFGDRVALADQHGSHipagshTYRDLYSRSLSLAREICRlracsDGDLREERVSFLCSNDVSY 114
Cdd:PRK13383 30 ASRGgtNPYTLLAVTAARWPGRTAIIDDDGAL------SYRELQRATESLARRLTR-----DGVAPGRAVGVMCRNGRGF 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 115 VVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPLLPLPPTVYCGAAKDPGEEqvp 194
Cdd:PRK13383 99 VTAVFAVGLLGADVVPISTEFRSDALAAALRAHHISTVVADNEFAERIAGADDAVAVIDPATAGAEESGGRPAVAAP--- 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 195 erdwrdrGAMIIYTSGTTGRPKGV-----VITHHNVWATvtgLVHKWAWTKDDVILHVLPLHHVHGVvNKLLCPLWVGAT 269
Cdd:PRK13383 176 -------GRIVLLTSGTTGKPKGVprapqLRSAVGVWVT---ILDRTRLRTGSRISVAMPMFHGLGL-GMLMLTIALGGT 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 270 CVMLPEFSAqlvwEKFLSSQS-PRVNVFMAVPTIYSKLMDYHDRhfgqphvrdfVRAVCE-EKIRLMVSGSAALPLPVLE 347
Cdd:PRK13383 245 VLTHRHFDA----EAALAQASlHRADAFTAVPVVLARILELPPR----------VRARNPlPQLRVVMSSGDRLDPTLGQ 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 348 KWKGATGHTLLERYGMTEIGM-ALSNPLATARLPGSVGTPLPGVEVRIVSENPQKDGcpyiihaegneteTRVTpgfeet 426
Cdd:PRK13383 311 RFMDTYGDILYNGYGSTEVGIgALATPADLRDAPETVGKPVAGCPVRILDRNNRPVG-------------PRVT------ 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 427 eGELLVRGPSVFRGYWDKPEETknafTSDGWFKTGDTAVFKD-GSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIA 505
Cdd:PRK13383 372 -GRIFVGGELAGTRYTDGGGKA----VVDGMTSTGDMGYLDNaGRLFIVGRED-DMIISGGENVYPRAVENALAAHPAVA 445
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 743747175 506 DVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK13383 446 DNAVIGVPDERFGHRLAAFVVLHPGSGVDAAQLRDYLKDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
203-571 |
2.04e-39 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 151.49 E-value: 2.04e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKgvVITHHNVWA---TVTGLVhkWAWTKDDVI-LHVLPLHHVHGVVNKLLCPlWVGATCVMLPE--- 275
Cdd:cd05970 188 LLVYFSSGTTGMPK--MVEHDFTYPlghIVTAKY--WQNVREGGLhLTVADTGWGKAVWGKIYGQ-WIAGAAVFVYDydk 262
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 276 FSAQLVWEKFlssQSPRVNVFMAVPTIYSKLMDYHDRHFgqphvrDFvravceEKIRLMVSGSAALPLPVLEKWKGATGH 355
Cdd:cd05970 263 FDPKALLEKL---SKYGVTTFCAPPTIYRFLIREDLSRY------DL------SSLRYCTTAGEALNPEVFNTFKEKTGI 327
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 356 TLLERYGMTEIGMALSN-PLATARlPGSVGTPLPGVEVRIvsenpqkdgcpyiIHAEGNETETrvtpgfeETEGELLVR- 433
Cdd:cd05970 328 KLMEGFGQTETTLTIATfPWMEPK-PGSMGKPAPGYEIDL-------------IDREGRSCEA-------GEEGEIVIRt 386
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 434 --GPSV--FRGYWDKPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVA 508
Cdd:cd05970 387 skGKPVglFGGYYKDAEKTAEVW-HDGYYHTGDAAWMdEDGYLWFVGRTD-DLIKSSGYRIGPFEVESALIQHPAVLECA 464
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 509 VIGVPDVTWGQRVTAVVTLREGHSLSH---RELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNK 571
Cdd:cd05970 465 VTGVPDPIRGQVVKATIVLAKGYEPSEelkKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRR 530
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
73-578 |
3.92e-39 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 150.67 E-value: 3.92e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRlracsDGDLREERVSFLCSNDVSYVVAqWASWMSGG----TAVPlyRKHPQaELEYFIQDSR 148
Cdd:PRK06018 41 TYAQIHDRALKVSQALDR-----DGIKLGDRVATIAWNTWRHLEA-WYGIMGIGaichTVNP--RLFPE-QIAWIINHAE 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 149 SSVVLAGQEHVELLSPVVRKLgvpLLPLPPTVYCGAAKDPG---------EEQVPERD----WRD----RGAMIIYTSGT 211
Cdd:PRK06018 112 DRVVITDLTFVPILEKIADKL---PSVERYVVLTDAAHMPQttlknavayEEWIAEADgdfaWKTfdenTAAGMCYTSGT 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 212 TGRPKGVVITHH-NVW-ATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLwVGATCVML-PEFSAQLVWEkFLSS 288
Cdd:PRK06018 189 TGDPKGVLYSHRsNVLhALMANNGDALGTSAADTMLPVVPLFHANSWGIAFSAPS-MGTKLVMPgAKLDGASVYE-LLDT 266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 289 QspRVNVFMAVPTIYSKLMDYHDRHFGQ-PHVRdfvRAVCeekirlmvsGSAALPLPVLEKWKgATGHTLLERYGMTE-- 365
Cdd:PRK06018 267 E--KVTFTAGVPTVWLMLLQYMEKEGLKlPHLK---MVVC---------GGSAMPRSMIKAFE-DMGVEVRHAWGMTEms 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 366 -IGMALSNPLATARLPGSV--------GTPLPGVEVRIVSEnpqkdgcpyiihaEGNETetrvtPGFEETEGELLVRGPS 436
Cdd:PRK06018 332 pLGTLAALKPPFSKLPGDArldvlqkqGYPPFGVEMKITDD-------------AGKEL-----PWDGKTFGRLKVRGPA 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 437 VFRGYWdkpEETKNAFTSDGWFKTGDTA-VFKDGSYWIRGRtSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDV 515
Cdd:PRK06018 394 VAAAYY---RVDGEILDDDGFFDTGDVAtIDAYGYMRITDR-SKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHP 469
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 743747175 516 TWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK06018 470 KWDERPLLIVQLKPGETATREEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALREQF 532
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
203-577 |
1.46e-38 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 149.15 E-value: 1.46e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIY-TSGTTGRPKGVVITHHNVWATVTGLVHKW-AWTKDDVILHVLPLHHVHGVVNKLLCPlWVGATCVM---LPEFS 277
Cdd:cd05928 176 PMAIYfTSGTTGSPKMAEHSHSSLGLGLKVNGRYWlDLTASDIMWNTSDTGWIKSAWSSLFEP-WIQGACVFvhhLPRFD 254
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 278 AQLVWEKFlsSQSPrVNVFMAVPTIYSKLMDYHDRHFGQPHVRDfvravCeekirlmVSGSAALPLPVLEKWKGATGHTL 357
Cdd:cd05928 255 PLVILKTL--SSYP-ITTFCGAPTVYRMLVQQDLSSYKFPSLQH-----C-------VTGGEPLNPEVLEKWKAQTGLDI 319
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 358 LERYGMTEIGMALSNPLATARLPGSVGTPLPGVEVRIVSENpqkdgcpyiihaeGNetetRVTPGfeeTEGELLVR-GP- 435
Cdd:cd05928 320 YEGYGQTETGLICANFKGMKIKPGSMGKASPPYDVQIIDDN-------------GN----VLPPG---TEGDIGIRvKPi 379
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 436 ---SVFRGYWDKPEETKNAFTSDGWFkTGDTAVF-KDGSYWIRGRtSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIG 511
Cdd:cd05928 380 rpfGLFSGYVDNPEKTAATIRGDFYL-TGDRGIMdEDGYFWFMGR-ADDVINSSGYRIGPFEVESALIEHPAVVESAVVS 457
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 743747175 512 VPDVTWGQRVTAVVTLREGHsLSH------RELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQ 577
Cdd:cd05928 458 SPDPIRGEVVKAFVVLAPQF-LSHdpeqltKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRDK 528
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
103-546 |
2.05e-38 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 148.39 E-value: 2.05e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 103 RVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQ-EHVELLSPVV-RKLGVPLLPLPPTV 180
Cdd:cd05932 33 KIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFVGKlDDWKAMAPGVpEGLISISLPPPSAA 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 181 YCG------AAKDPGEEQVPERDwRDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVH 254
Cdd:cd05932 113 NCQyqwddlIAQHPPLEERPTRF-PEQLATLIYTSGTTGQPKGVMLTFGSFAWAAQAGIEHIGTEENDRMLSYLPLAHVT 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 255 GVVNKLLCPLWVGATcVMLPE----FSAQLvwekflssQSPRVNVFMAVPTIYSKL-MDYHDRHFGQ--------PHVRD 321
Cdd:cd05932 192 ERVFVEGGSLYGGVL-VAFAEsldtFVEDV--------QRARPTLFFSVPRLWTKFqQGVQDKIPQQklnlllkiPVVNS 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 322 FVR-----AVCEEKIRLMVSGSAALPLPVLEkWKGATGHTLLERYGMTE-IGMALSNPLATARLpGSVGTPLPGVEVRIv 395
Cdd:cd05932 263 LVKrkvlkGLGLDQCRLAGCGSAPVPPALLE-WYRSLGLNILEAYGMTEnFAYSHLNYPGRDKI-GTVGNAGPGVEVRI- 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 396 senpqkdgcpyiihaegnetetrvtpgfeETEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDT-AVFKDGSYWIR 474
Cdd:cd05932 340 -----------------------------SEDGEILVRSPALMMGYYKDPEATAEAFTADGFLRTGDKgELDADGNLTIT 390
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 743747175 475 GRTSvDIIKTG-GYKVSALEVERLLLGHPSIADVAVIgvpdvtwGQRVTAVVTLREGHSLSHRELKEWARGVL 546
Cdd:cd05932 391 GRVK-DIFKTSkGKYVAPAPIENKLAEHDRVEMVCVI-------GSGLPAPLALVVLSEEARLRADAFARAEL 455
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
104-581 |
2.78e-38 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 147.92 E-value: 2.78e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 104 VSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAgqeHVELLSPV-------VRKLGVPLLPL 176
Cdd:PRK12406 39 VALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLIA---HADLLHGLasalpagVTVLSVPTPPE 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 177 PPTVYcgaAKDPGEEQVPE--RDWRD---------------RGAMIiYTSGTTGRPKGV-----VITHHNVWATVTGLVH 234
Cdd:PRK12406 116 IAAAY---RISPALLTPPAgaIDWEGwlaqqepydgppvpqPQSMI-YTSGTTGHPKGVrraapTPEQAAAAEQMRALIY 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 235 kwAWTKDDVILHVLPLHH----VHGVVNKLLcplwvGATCVMLPEFSAqlvwEKFLSS-QSPRV-NVFMaVPTIYSKLMD 308
Cdd:PRK12406 192 --GLKPGIRALLTGPLYHsapnAYGLRAGRL-----GGVLVLQPRFDP----EELLQLiERHRItHMHM-VPTMFIRLLK 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 309 YHDRhfgqphvrdfVRAVCE-EKIRLMVSGSAALPLPV----LEKWkgatGHTLLERYGMTEIG-MALSNPLATARLPGS 382
Cdd:PRK12406 260 LPEE----------VRAKYDvSSLRHVIHAAAPCPADVkramIEWW----GPVIYEYYGSTESGaVTFATSEDALSHPGT 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 383 VGTPLPGVEVRIVSEnpqkdgcpyiihaEGNEtetrVTPGfeeTEGELLVRGPSV--FRgYWDKPEEtKNAFTSDGWFKT 460
Cdd:PRK12406 326 VGKAAPGAELRFVDE-------------DGRP----LPQG---EIGEIYSRIAGNpdFT-YHNKPEK-RAEIDRGGFITS 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 461 GDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELK 539
Cdd:PRK12406 384 GDVGYLdADGYLFLCDRKR-DMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEPQPGATLDEADIR 462
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 743747175 540 EWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQLYPQ 581
Cdd:PRK12406 463 AQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLRDPYWAN 504
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
55-574 |
4.79e-38 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 145.92 E-value: 4.79e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADQHGSHipagshTYRDLYSRSLSLAReicRLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRK 134
Cdd:cd12115 14 DAIALVCGDESL------TYAELNRRANRLAA---RLRA--AGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPA 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 135 HPQAELEYFIQDSRSSVVLAGQEHVellspvvrklgvpllplpptvycgaakdpgeeqvperdwrdrgAMIIYTSGTTGR 214
Cdd:cd12115 83 YPPERLRFILEDAQARLVLTDPDDL-------------------------------------------AYVIYTSGSTGR 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 215 PKGVVITHHNVWATVtglvhKWAwtkddviLHVLPLHHVHGV-----------VNKLLCPLWVGATCVMlpefsAQLVWE 283
Cdd:cd12115 120 PKGVAIEHRNAAAFL-----QWA-------AAAFSAEELAGVlastsicfdlsVFELFGPLATGGKVVL-----ADNVLA 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 KFLSSQSPRVNVFMAVPTIYSKLMdyhdRHFGQPHVrdfVRAVCeekirlmvsgSAALPLP---VLEKWKGATGHTLLER 360
Cdd:cd12115 183 LPDLPAAAEVTLINTVPSAAAELL----RHDALPAS---VRVVN----------LAGEPLPrdlVQRLYARLQVERVVNL 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 361 YGMTE---IGMALSNPLATARLPgSVGTPLPGVEVRIVSENpqkdgcpyiihaegnetETRVTPGfeeTEGELLVRGPSV 437
Cdd:cd12115 246 YGPSEdttYSTVAPVPPGASGEV-SIGRPLANTQAYVLDRA-----------------LQPVPLG---VPGELYIGGAGV 304
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 438 FRGYWDKPEETKNAFTSDGW------FKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVI 510
Cdd:cd12115 305 ARGYLGRPGLTAERFLPDPFgpgarlYRTGDLVRWRpDGLLEFLGRAD-NQVKVRGFRIELGEIEAALRSIPGVREAVVV 383
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 743747175 511 GVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd12115 384 AIGDAAGERRLVAYIVAEPGAAGLVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
49-577 |
5.79e-38 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 147.13 E-value: 5.79e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVAladQHGSHIPAGshtyrdlysrslslAREICRLRACSDGDlREERVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:PRK07867 20 RGLYFEDSFT---SWREHIRGS--------------AARAAALRARLDPT-RPPHVGVLLDNTPEFSLLLGAAALSGIVP 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELL---SPVVRKLGVPLLPLPPTVycgaAKDPGEEQVPERDWRDRGAMI 205
Cdd:PRK07867 82 VGLNPTRRGAALARDIAHADCQLVLTESAHAELLdglDPGVRVINVDSPAWADEL----AAHRDAEPPFRVADPDDLFML 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 206 IYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQlvweKF 285
Cdd:PRK07867 158 IFTSGTSGDPKAVRCTHRKVASAGVMLAQRFGLGPDDVCYVSMPLFHSNAVMAGWAVALAAGASIALRRKFSAS----GF 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 286 LssqsPRVNVFMAVPTIY-SKLMDYHDRHFGQPHVRDfvravceEKIRLMVsGSAALPlPVLEKWKGATGHTLLERYGMT 364
Cdd:PRK07867 234 L----PDVRRYGATYANYvGKPLSYVLATPERPDDAD-------NPLRIVY-GNEGAP-GDIARFARRFGCVVVDGFGST 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 365 EIGMALSNPLATArlPGSVGTPLPGVEVRivseNPQK-DGCPyiihaEGNETETRVTPGFEETeGELL-VRGPSVFRGYW 442
Cdd:PRK07867 301 EGGVAITRTPDTP--PGALGPLPPGVAIV----DPDTgTECP-----PAEDADGRLLNADEAI-GELVnTAGPGGFEGYY 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 443 DKPEETKnAFTSDGWFKTGDTAvFKD--GSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQR 520
Cdd:PRK07867 369 NDPEADA-ERMRGGVYWSGDLA-YRDadGYAYFAGRLG-DWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQ 445
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 743747175 521 VTAVVTLREGHSLSHRELKEW--ARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQ 577
Cdd:PRK07867 446 VMAALVLAPGAKFDPDAFAEFlaAQPDLGPKQWPSYVRVCAELPRTATFKVLKRQLSAE 504
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
205-574 |
5.79e-38 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 148.48 E-value: 5.79e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITH--HNVWATVTglvHKWA---------WTKDDV--------IlhvlplhhVHGvvnkllcPLW 265
Cdd:cd05966 236 ILYTSGSTGKPKGVVHTTggYLLYAATT---FKYVfdyhpddiyWCTADIgwitghsyI--------VYG-------PLA 297
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 266 VGATCVML------PEFSA--QLVwEKFlssqspRVNVFMAVPTIYSKLMDYHDrhfgqphvrDFVRAVCEEKIRLMvsG 337
Cdd:cd05966 298 NGATTVMFegtptyPDPGRywDIV-EKH------KVTIFYTAPTAIRALMKFGD---------EWVKKHDLSSLRVL--G 359
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 338 SAALPL-PvlEKWKGATGHTLLER------YGMTEIGMALSNPL--ATARLPGSVGTPLPGVEVRIVSENpqkdgcpyii 408
Cdd:cd05966 360 SVGEPInP--EAWMWYYEVIGKERcpivdtWWQTETGGIMITPLpgATPLKPGSATRPFFGIEPAILDEE---------- 427
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 409 haeGNETETrvtpgfeETEGELLVRG--PSVFRGYWDKPEETKNAFTSD--GWFKTGDTAVF-KDGSYWIRGRTSvDIIK 483
Cdd:cd05966 428 ---GNEVEG-------EVEGYLVIKRpwPGMARTIYGDHERYEDTYFSKfpGYYFTGDGARRdEDGYYWITGRVD-DVIN 496
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 484 TGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHR---ELKEWARGVLAPYAVPSELLLVEE 560
Cdd:cd05966 497 VSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEPSDElrkELRKHVRKEIGPIATPDKIQFVPG 576
|
410
....*....|....
gi 743747175 561 IPRNQMGKVNKRDL 574
Cdd:cd05966 577 LPKTRSGKIMRRIL 590
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
203-543 |
1.14e-37 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 146.21 E-value: 1.14e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLVH---KWAwTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLP----- 274
Cdd:cd17639 91 ACIMYTSGSTGNPKGVMLTHGNLVAGIAGLGDrvpELL-GPDDRYLAYLPLAHIFELAAENVCLYRGGTIGYGSPrtltd 169
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 275 -----------EFSAQL------VWEKFLSSQSPRVNvfmAVPTIYSKLMD--YHDRHFGQPHVRD--------F--VRA 325
Cdd:cd17639 170 kskrgckgdltEFKPTLmvgvpaIWDTIRKGVLAKLN---PMGGLKRTLFWtaYQSKLKALKEGPGtplldelvFkkVRA 246
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 326 VCEEKIRLMVSGSAALPlPVLEKWKGATGHTLLERYGMTEI--GMALSNPLATArlPGSVGTPLPGVEVRIVSenpqkdg 403
Cdd:cd17639 247 ALGGRLRYMLSGGAPLS-ADTQEFLNIVLCPVIQGYGLTETcaGGTVQDPGDLE--TGRVGPPLPCCEIKLVD------- 316
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 404 CPyiihaEGNETETRVTPgfeetEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDII 482
Cdd:cd17639 317 WE-----EGGYSTDKPPP-----RGEILIRGPNVFKGYYKNPEKTKEAFDGDGWFHTGDIGEFhPDGTLKIIDRKK-DLV 385
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 743747175 483 KT--GGYkvSALE-VERLLLGHPSIADVAVIGVPDVTwgqRVTAVVTLREGHslshreLKEWAR 543
Cdd:cd17639 386 KLqnGEY--IALEkLESIYRSNPLVNNICVYADPDKS---YPVAIVVPNEKH------LTKLAE 438
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
73-495 |
2.60e-37 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 145.50 E-value: 2.60e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAReicRLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPL-------YRKHPQAELEYFIQ 145
Cdd:cd05906 41 SYQDLLEDARRLAA---GLRQ--LGLRPGDSVILQFDDNEDFIPAFWACVLAGFVPAPLtvpptydEPNARLRKLRHIWQ 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 146 DSRSSVVLAGQEhvelLSPVVRKLGVPLLPLPPTVYCGAAKDPGEEQVPERDWR-DRGAMIIYTSGTTGRPKGVVITHHN 224
Cdd:cd05906 116 LLGSPVVLTDAE----LVAEFAGLETLSGLPGIRVLSIEELLDTAADHDLPQSRpDDLALLMLTSGSTGFPKAVPLTHRN 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 225 VWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEfsaqlvwEKFLSSqsprvnvfmavPTiys 304
Cdd:cd05906 192 ILARSAGKIQHNGLTPQDVFLNWVPLDHVGGLVELHLRAVYLGCQQVHVPT-------EEILAD-----------PL--- 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 305 KLMDYHDRH---------FGQPHVRDFVRAVCEEK-----IRLMVSGSAALPLPVLEKWKGatghtLLERY--------- 361
Cdd:cd05906 251 RWLDLIDRYrvtitwapnFAFALLNDLLEEIEDGTwdlssLRYLVNAGEAVVAKTIRRLLR-----LLEPYglppdairp 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 362 --GMTEI--GMALSNPLATARLPG-----SVGTPLPGVEVRIVSENPQkdgcpyiihaegnetetrVTPgfEETEGELLV 432
Cdd:cd05906 326 afGMTETcsGVIYSRSFPTYDHSQalefvSLGRPIPGVSMRIVDDEGQ------------------LLP--EGEVGRLQV 385
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 743747175 433 RGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFKDGSYWIRGRTSvDIIKTGGYKVSALEVE 495
Cdd:cd05906 386 RGPVVTKGYYNNPEANAEAFTEDGWFRTGDLGFLDNGNLTITGRTK-DTIIVNGVNYYSHEIE 447
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
73-571 |
3.82e-37 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 143.43 E-value: 3.82e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLrACSDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKH-PQA-ELEYFIQDSRSS 150
Cdd:cd05973 2 TFGELRALSARFANALQEL-GVGPGD----VVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFgPKAiEHRLRTSGARLV 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 151 VVLAGQEHvellspvvrKLgvpllplpptvycgaAKDPgeeqvperdwrdrgAMIIYTSGTTGRPKGVVITHHNVWATVT 230
Cdd:cd05973 77 VTDAANRH---------KL---------------DSDP--------------FVMMFTSGTTGLPKGVPVPLRALAAFGA 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 231 GLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLP-EFSAQLVW---EKFlssqspRVNVFMAVPTIYSKL 306
Cdd:cd05973 119 YLRDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPTILLEgGFSVESTWrviERL------GVTNLAGSPTAYRLL 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 307 MDyhdrhfgqphvrDFVRAVCEEKIRLMVSGSAALPL-PVLEKWKGAT-GHTLLERYGMTEIGMALSNPLATARL--PGS 382
Cdd:cd05973 193 MA------------AGAEVPARPKGRLRRVSSAGEPLtPEVIRWFDAAlGVPIHDHYGQTELGMVLANHHALEHPvhAGS 260
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 383 VGTPLPGVEVRIVSENpqkdgcpyiihaeGNETETRVtPGFEETEgelLVRGPSV-FRGYWDKPEETknafTSDGWFKTG 461
Cdd:cd05973 261 AGRAMPGWRVAVLDDD-------------GDELGPGE-PGRLAID---IANSPLMwFRGYQLPDTPA----IDGGYYLTG 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 462 DTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLS---HRE 537
Cdd:cd05973 320 DTVEFDpDGSFSFIGRAD-DVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTpalADE 398
|
490 500 510
....*....|....*....|....*....|....
gi 743747175 538 LKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNK 571
Cdd:cd05973 399 LQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQR 432
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
73-578 |
4.61e-37 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 144.15 E-value: 4.61e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYsRSLSLAREICRLRACsdgdlREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PRK07638 28 TYKDWF-ESVCKVANWLNEKES-----KNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDELKERLAISNADMI 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHVELLSPVvrklgvpllplpptvycgaakdpgEEQVPE-RDWR-------DRGAMII----------YTSGTTGR 214
Cdd:PRK07638 102 VTERYKLNDLPDE------------------------EGRVIEiDEWKrmiekylPTYAPIEnvqnapfymgFTSGSTGK 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 215 PKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVH---GVVNKLlcplWVGATCVMLPEFSAQLVWEKFlssQSP 291
Cdd:PRK07638 158 PKAFLRAQQSWLHSFDCNVHDFHMKREDSVLIAGTLVHSLflyGAISTL----YVGQTVHLMRKFIPNQVLDKL---ETE 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 292 RVNVFMAVPTIYSKLMDyhdrhfgqphvrdfVRAVCEEKIRLMVSGsAALPLPVLEKWKGATGH-TLLERYGMTEIG-MA 369
Cdd:PRK07638 231 NISVMYTVPTMLESLYK--------------ENRVIENKMKIISSG-AKWEAEAKEKIKNIFPYaKLYEFYGASELSfVT 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 370 LSNPLATARLPGSVGTPLPGVEVRIVsenpqkdgcpyiihaegNETETRVTPGfeeTEGELLVRGPSVFRGYWDKPEETK 449
Cdd:PRK07638 296 ALVDEESERRPNSVGRPFHNVQVRIC-----------------NEAGEEVQKG---EIGTVYVKSPQFFMGYIIGGVLAR 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 450 NaFTSDGWFKTGDTA-VFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVtlr 528
Cdd:PRK07638 356 E-LNADGWMTVRDVGyEDEEGFIYIVGREK-NMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAII--- 430
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 743747175 529 EGHSLShRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK07638 431 KGSATK-QQLKSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSWI 479
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
73-511 |
1.06e-36 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 143.90 E-value: 1.06e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLRACSDGDlreERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRK-HPQAeLEYFIQDSRSSV 151
Cdd:cd05927 7 SYKEVAERADNIGSALRSLGGKPAPA---SFVGIYSINRPEWIISELACYAYSLVTVPLYDTlGPEA-IEYILNHAEISI 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 152 VLAgQEHVELLS-PVVRKLGvpllplpptvycgaAKDPGEEQVPERDwrdRGAMIIYTSGTTGRPKGVVITHHNVWATVT 230
Cdd:cd05927 83 VFC-DAGVKVYSlEEFEKLG--------------KKNKVPPPPPKPE---DLATICYTSGTTGNPKGVMLTHGNIVSNVA 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 231 GLVHKW----AWTKDDVILHVLPLHHV--HGVVNKLLC-----PLWVGATCVMLPEFsaqlvwekflssQSPRVNVFMAV 299
Cdd:cd05927 145 GVFKILeilnKINPTDVYISYLPLAHIfeRVVEALFLYhgakiGFYSGDIRLLLDDI------------KALKPTVFPGV 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 300 PTIYSKL----------------------MDYHDRHFGQPHVR-----DF-----VRAVCEEKIRLMVSGSAALPLPVLE 347
Cdd:cd05927 213 PRVLNRIydkifnkvqakgplkrklfnfaLNYKLAELRSGVVRaspfwDKlvfnkIKQALGGNVRLMLTGSAPLSPEVLE 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 348 KWKGATGHTLLERYGMTEI--GMALSNPLATArlPGSVGTPLPGVEVRIVSenpqkdgCPyiihaEGNETETRVTPgfee 425
Cdd:cd05927 293 FLRVALGCPVLEGYGQTECtaGATLTLPGDTS--VGHVGGPLPCAEVKLVD-------VP-----EMNYDAKDPNP---- 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 426 tEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIK--TGGYkVSALEVERLLLGHP 502
Cdd:cd05927 355 -RGEVCIRGPNVFSGYYKDPEKTAEALDEDGWLHTGDIGEWlPNGTLKIIDRKK-NIFKlsQGEY-VAPEKIENIYARSP 431
|
....*....
gi 743747175 503 SIADVAVIG 511
Cdd:cd05927 432 FVAQIFVYG 440
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
49-575 |
4.16e-36 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 140.98 E-value: 4.16e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALADQHGSHIpagshtyRDLYSRSLSLAREICRLRACSDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:cd05929 1 LEARDLDRAQVFHQRRLLL-------LDVYSIALNRNARAAAAEGVWIAD----GVYIYLINSILTVFAAAAAWKCGACP 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLYRKHPQAELEYFIQdsrssvvlagqehvelLSPVVRKLGVPLLPLPPTVYCGAAKDPGEEQVPERDWRDRGAMIIYT 208
Cdd:cd05929 70 AYKSSRAPRAEACAIIE----------------IKAAALVCGLFTGGGALDGLEDYEAAEGGSPETPIEDEAAGWKMLYS 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 209 SGTTGRPKGVVITHHNVW---ATVTGLVHKWAWTKDDVILHVLPLHHV--HGVVNKllcPLWVGATCVMLPEFSAqlvwE 283
Cdd:cd05929 134 GGTTGRPKGIKRGLPGGPpdnDTLMAAALGFGPGADSVYLSPAPLYHAapFRWSMT---ALFMGGTLVLMEKFDP----E 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 KFLSS-QSPRVNVFMAVPTIYSKLMDYHDRhfgQPHVRDFvravceEKIRLMVSGSAALPLPVLEKWKGATGHTLLERYG 362
Cdd:cd05929 207 EFLRLiERYRVTFAQFVPTMFVRLLKLPEA---VRNAYDL------SSLKRVIHAAAPCPPWVKEQWIDWGGPIIWEYYG 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 363 MTE-IGMALSNPLATARLPGSVGTPLPGvEVRIVSENpqkdgcpyiihaeGNEtetrVTPGfeeTEGELLVRGPSVFRgY 441
Cdd:cd05929 278 GTEgQGLTIINGEEWLTHPGSVGRAVLG-KVHILDED-------------GNE----VPPG---EIGEVYFANGPGFE-Y 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 442 WDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQR 520
Cdd:cd05929 336 TNDPEKTAAARNEGGWSTLGDVGYLdEDGYLYLTDRRS-DMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQR 414
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 743747175 521 VTAVVT----LREGHSLSHrELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLV 575
Cdd:cd05929 415 VHAVVQpapgADAGTALAE-ELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLR 472
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
72-578 |
6.94e-36 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 141.38 E-value: 6.94e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 72 HTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWAswMSGGTAV-----PlyRKHPQaELEYFIQD 146
Cdd:PRK07008 40 YTYRDCERRAKQLAQALAAL-----GVEPGDRVGTLAWNGYRHLEAYYG--VSGSGAVchtinP--RLFPE-QIAYIVNH 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 147 SRSSVVLAGQEHVELLS------PVVRKLgvpllplppTVYCGAAKDPGEE------------QVPERDW----RDRGAM 204
Cdd:PRK07008 110 AEDRYVLFDLTFLPLVDalapqcPNVKGW---------VAMTDAAHLPAGStpllcyetlvgaQDGDYDWprfdENQASS 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITHHnvwATVtglVHKWA--------WTKDDVILHVLPLHHVHGVVNKLLCPLwVGATCVML-PE 275
Cdd:PRK07008 181 LCYTSGTTGNPKGALYSHR---STV---LHAYGaalpdamgLSARDAVLPVVPMFHVNAWGLPYSAPL-TGAKLVLPgPD 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 276 FSAQLVWEKFlssQSPRVNVFMAVPTIYSKLMDYhdrhfgqphvrdfVRA--VCEEKIRLMVSGSAALPLPVLEKWKGAT 353
Cdd:PRK07008 254 LDGKSLYELI---EAERVTFSAGVPTVWLGLLNH-------------MREagLRFSTLRRTVIGGSACPPAMIRTFEDEY 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 354 GHTLLERYGMTEIgmalsNPLAT--------ARLPGSV--------GTPLPGVEVRIVSENPQK---DGCPYiihaegne 414
Cdd:PRK07008 318 GVEVIHAWGMTEM-----SPLGTlcklkwkhSQLPLDEqrkllekqGRVIYGVDMKIVGDDGRElpwDGKAF-------- 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 415 tetrvtpgfeeteGELLVRGPSVFRGYWDKPEETknafTSDGWFKTGDTAVF-KDGSYWIRGRtSVDIIKTGGYKVSALE 493
Cdd:PRK07008 385 -------------GDLQVRGPWVIDRYFRGDASP----LVDGWFPTGDVATIdADGFMQITDR-SKDVIKSGGEWISSID 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 494 VERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRD 573
Cdd:PRK07008 447 IENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGAEVTREELLAFYEGKVAKWWIPDDVVFVDAIPHTATGKLQKLK 526
|
....*
gi 743747175 574 LVQQL 578
Cdd:PRK07008 527 LREQF 531
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
73-577 |
7.02e-36 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 140.98 E-value: 7.02e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAReICRLRACSDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PRK13391 26 TYRELDERSNRLAH-LFRSLGLKRGD----HVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARAL 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHVEllspVVRKL-GVPLLPLPPTVYCGAAKDPGEEQVPE---------RDWRDRGAMIIYTSGTTGRPKGVV--I 220
Cdd:PRK13391 101 ITSAAKLD----VARALlKQCPGVRHRLVLDGDGELEGFVGYAEavaglpatpIADESLGTDMLYSSGTTGRPKGIKrpL 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 221 THHNVWAT--VTGLVHK-WAWTKDDVILHVLPLHHVH-----GVVNKLlcplwvGATCVMLPEFSAqlvwEKFLSS-QSP 291
Cdd:PRK13391 177 PEQPPDTPlpLTAFLQRlWGFRSDMVYLSPAPLYHSApqravMLVIRL------GGTVIVMEHFDA----EQYLALiEEY 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 292 RVNVFMAVPTIYSKLMDYHDRHFGQPHVRDFVRAVceekirlmvsgSAALPLPVLEK-----WKGATGHtllERYGMTE- 365
Cdd:PRK13391 247 GVTHTQLVPTMFSRMLKLPEEVRDKYDLSSLEVAI-----------HAAAPCPPQVKeqmidWWGPIIH---EYYAATEg 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 366 IGM-ALSNPLATARlPGSVGTPLPGVeVRIVSENPQKdgCPyiihaegnetetrvtPGfeeTEGELLVRGPSVFRgYWDK 444
Cdd:PRK13391 313 LGFtACDSEEWLAH-PGTVGRAMFGD-LHILDDDGAE--LP---------------PG---EPGTIWFEGGRPFE-YLND 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 445 PEETKNAFTSDG-WFKTGDTA-VFKDGSYWIRGRTSVDIIkTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVT 522
Cdd:PRK13391 370 PAKTAEARHPDGtWSTVGDIGyVDEDGYLYLTDRAAFMII-SGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVK 448
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 743747175 523 AVVTLREGHSLS---HRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQ 577
Cdd:PRK13391 449 AVVQPVDGVDPGpalAAELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLRDR 506
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
192-578 |
7.28e-35 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 138.76 E-value: 7.28e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 192 QVPERDwrdrGAMIIYTSGTTGRPKGVVITHHNVWATVTGL--VHKWAWTKDDVILHVLPLHHV--HGVvnkllcPL--W 265
Cdd:PRK05620 177 ELDETT----AAAICYSTGTTGAPKGVVYSHRSLYLQSLSLrtTDSLAVTHGESFLCCVPIYHVlsWGV------PLaaF 246
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 266 VGATCVMLP--EFSAQLVwEKFLSSQSPRVNvfMAVPTIYSKLMDYHDRHfgqPHVRdfvravceEKIRLMVSGSAALPL 343
Cdd:PRK05620 247 MSGTPLVFPgpDLSAPTL-AKIIATAMPRVA--HGVPTLWIQLMVHYLKN---PPER--------MSLQEIYVGGSAVPP 312
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 344 PVLEKWKgatghtllERYGMTEI---GMALSNPLAT-ARLPGSV------------GTPLPGVEVRIVSenpqkDGcpyi 407
Cdd:PRK05620 313 ILIKAWE--------ERYGVDVVhvwGMTETSPVGTvARPPSGVsgearwayrvsqGRFPASLEYRIVN-----DG---- 375
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 408 ihaegnetetRVTPGFEETEGELLVRGPSVFRGYWDKP----------------EETKNAFTSDGWFKTGDTA-VFKDGS 470
Cdd:PRK05620 376 ----------QVMESTDRNEGEIQVRGNWVTASYYHSPteegggaastfrgedvEDANDRFTADGWLRTGDVGsVTRDGF 445
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 471 YWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSH---RELKEWARGVLA 547
Cdd:PRK05620 446 LTIHDRAR-DVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTRetaERLRDQLRDRLP 524
|
410 420 430
....*....|....*....|....*....|.
gi 743747175 548 PYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK05620 525 NWMLPEYWTFVDEIDKTSVGKFDKKDLRQHL 555
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
73-583 |
1.05e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 137.95 E-value: 1.05e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLRACsdgdlREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PRK06164 37 SRAELRALVDRLAAWLAAQGVR-----RGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRYRSHEVAHILGRGRARWL 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 --------------LAGQEHVELlsPVVRKLGVPLLPLPPT----------VYCGAAKDPGEEQVPERDWRDRGAMIIYT 208
Cdd:PRK06164 112 vvwpgfkgidfaaiLAAVPPDAL--PPLRAIAVVDDAADATpapapgarvqLFALPDPAPPAAAGERAADPDAGALLFTT 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 209 SGTTGRPKGV------VITHHNVWATVTGLvhkwawTKDDVILHVLPLHHVHGVvNKLLCPLWVGATCVMLPEF----SA 278
Cdd:PRK06164 190 SGTTSGPKLVlhrqatLLRHARAIARAYGY------DPGAVLLAALPFCGVFGF-STLLGALAGGAPLVCEPVFdaarTA 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 QLVwekflssQSPRVNVFMAVPTIYSKLMDYHDRHfgqphvRDFVRAvceekiRLMVSGSAALPLPVLEKWKGATGHTLL 358
Cdd:PRK06164 263 RAL-------RRHRVTHTFGNDEMLRRILDTAGER------ADFPSA------RLFGFASFAPALGELAALARARGVPLT 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 359 ERYGMTEI-----GMALSNPLATARLPGsvGTPL-PGVEVRIVseNPQKDG-CPyiihaegnetetrvtPGfeeTEGELL 431
Cdd:PRK06164 324 GLYGSSEVqalvaLQPATDPVSVRIEGG--GRPAsPEARVRAR--DPQDGAlLP---------------DG---ESGEIE 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 432 VRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVI 510
Cdd:PRK06164 382 IRAPSLMRGYLDNPDATARALTDDGYFRTGDLGYTRgDGQFVYQTRMG-DSLRLGGFLVNPAEIEHALEALPGVAAAQVV 460
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 743747175 511 GVpDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNK------RDLVQQLYPQDR 583
Cdd:PRK06164 461 GA-TRDGKTVPVAFVIPTDGASPDEAGLMAACREALAGFKVPARVQVVEAFPVTESANGAKiqkhrlREMAQARLAAER 538
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
203-578 |
4.14e-34 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 132.86 E-value: 4.14e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVW----ATVTGLVHKWAWtkddviLHVLPLHHVHGVvNKLLCPLWVGATCVMLPefsa 278
Cdd:PRK07824 38 ALVVATSGTTGTPKGAMLTAAALTasadATHDRLGGPGQW------LLALPAHHIAGL-QVLVRSVIAGSEPVELD---- 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 qlVWEKFLSSQSPRVNVFMAVPTIYSKLMDYH-DRHFGQPhvrDFVRAVCEEKIRLMvsGSAALPLPVLEKWKGAtGHTL 357
Cdd:PRK07824 107 --VSAGFDPTALPRAVAELGGGRRYTSLVPMQlAKALDDP---AATAALAELDAVLV--GGGPAPAPVLDAAAAA-GINV 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 358 LERYGMTEigmalsnplaTArlPGSV--GTPLPGVEVRIVsenpqkdgcpyiihaegnetetrvtpgfeetEGELLVRGP 435
Cdd:PRK07824 179 VRTYGMSE----------TS--GGCVydGVPLDGVRVRVE-------------------------------DGRIALGGP 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 436 SVFRGYWDKPEEtkNAFTSDGWFKTGDTAVFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDV 515
Cdd:PRK07824 216 TLAKGYRNPVDP--DPFAEPGWFRTDDLGALDDGVLTVLGRAD-DAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDD 292
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 743747175 516 TWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK07824 293 RLGQRVVAAVVGDGGPAPTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALVRRF 355
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
48-578 |
7.16e-34 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 135.52 E-value: 7.16e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 48 TRALAFGDRVALADQHGSHIPAGSHTYRdlysrslslareicrlracsdgdlreERVSFLCSNDVSYVVAQWASWMSGGT 127
Cdd:PRK05857 39 TSALRYRELVAEVGGLAADLRAQSVSRG--------------------------SRVLVISDNGPETYLSVLACAKLGAI 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 128 AVPLYRKHPQAELEYFIQDSRSSVVL------AGQEHVELLSPVVRKLGVPLLPLPPTVYCGAAKDPGEEQvPERDWRDR 201
Cdd:PRK05857 93 AVMADGNLPIAAIERFCQITDPAAALvapgskMASSAVPEALHSIPVIAVDIAAVTRESEHSLDAASLAGN-ADQGSEDP 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 202 GAMIiYTSGTTGRPKGVVITHHNVWATVTGLVHK---WA-WTKDDVILHVLPLHHVHGVVNKLLCpLWVGATCVMLPEFS 277
Cdd:PRK05857 172 LAMI-FTSGTTGEPKAVLLANRTFFAVPDILQKEglnWVtWVVGETTYSPLPATHIGGLWWILTC-LMHGGLCVTGGENT 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 278 AQLVweKFLSSQspRVNVFMAVPTIYSKLMdyHDRHFGqphvrdfvrAVCEEKIRLMV-SGSAALPLPVleKWKGATGHT 356
Cdd:PRK05857 250 TSLL--EILTTN--AVATTCLVPTLLSKLV--SELKSA---------NATVPSLRLVGyGGSRAIAADV--RFIEATGVR 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 357 LLERYGMTEIG-MALSNPLATARLP----GSVGTPLPGVEVRIVSENpqkDGCPyiihaegneteTRVTPGFEETEGELL 431
Cdd:PRK05857 313 TAQVYGLSETGcTALCLPTDDGSIVkieaGAVGRPYPGVDVYLAATD---GIGP-----------TAPGAGPSASFGTLW 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 432 VRGPSVFRGYWDKPEETKNAFTsDGWFKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVI 510
Cdd:PRK05857 379 IKSPANMLGYWNNPERTAEVLI-DGWVNTGDLLERReDGFFYIKGRSS-EMIICGGVNIAPDEVDRIAEGVSGVREAACY 456
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 511 GVPDVTWGQRV-TAVVTLREGHSLSHRELKewaRGVLAPY-------AVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK05857 457 EIPDEEFGALVgLAVVASAELDESAARALK---HTIAARFrresepmARPSTIVIVTDIPRTQSGKVMRASLAAAA 529
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
50-574 |
1.57e-32 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 134.13 E-value: 1.57e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 50 ALAFGDRVAladqhgshipagshTYRDLYSRSLSLAReicRLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAV 129
Cdd:PRK12467 530 ALVFGEQVL--------------SYAELNRQANRLAH---VLIA--AGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYV 590
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 130 PLYRKHPQAELEYFIQDSRSSVVLaGQEHVELLSPVVRKLGVPLLPLPPTVYCG-AAKDPGEEQVPerdwrDRGAMIIYT 208
Cdd:PRK12467 591 PLDPEYPQDRLAYMLDDSGVRLLL-TQSHLLAQLPVPAGLRSLCLDEPADLLCGySGHNPEVALDP-----DNLAYVIYT 664
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 209 SGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLhHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWEKFLSS 288
Cdd:PRK12467 665 SGSTGQPKGVAISHGALANYVCVIAERLQLAADDSMLMVSTF-AFDLGVTELFGALASGATLHLLPPDCARDAEAFAALM 743
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 289 QSPRVNVFMAVPTIYSKLMDyhdrhfgqphvrDFVRAVCEEKIRLMVSGSaALPLPVLEKWKG-ATGHTLLERYGMTEIG 367
Cdd:PRK12467 744 ADQGVTVLKIVPSHLQALLQ------------ASRVALPRPQRALVCGGE-ALQVDLLARVRAlGPGARLINHYGPTETT 810
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 368 MALSN-PLATARLPGS---VGTPLPGVEVRIV--SENPQKDGCPyiihaegnetetrvtpgfeeteGELLVRGPSVFRGY 441
Cdd:PRK12467 811 VGVSTyELSDEERDFGnvpIGQPLANLGLYILdhYLNPVPVGVV----------------------GELYIGGAGLARGY 868
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 442 WDKPEETKNAFTSDGW-------FKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVP 513
Cdd:PRK12467 869 HRRPALTAERFVPDPFgadggrlYRTGDLARYrADGVIEYLGRMD-HQVKIRGFRIELGEIEARLLAQPGVREAVVLAQP 947
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 743747175 514 DVTWGQRVTAVVTLREGHSLSHR----ELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK12467 948 GDAGLQLVAYLVPAAVADGAEHQatrdELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKAL 1012
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
73-574 |
1.74e-32 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 130.28 E-value: 1.74e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:cd17650 14 TYRELNERANQLARTLRGL-----GVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYMLEDSGAKLL 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAgqehvellspvvrklgvpllplpptvycgaakDPGEEqvperdwrdrgAMIIYTSGTTGRPKGVVITHHNvwatVTGL 232
Cdd:cd17650 89 LT--------------------------------QPEDL-----------AYVIYTSGTTGKPKGVMVEHRN----VAHA 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 233 VHKWawtKDDVILHVLPLHHVHGV-------VNKLLCPLWVGATCVMLPE---FSAQLVWEKFLSSqspRVNVFMAVPTI 302
Cdd:cd17650 122 AHAW---RREYELDSFPVRLLQMAsfsfdvfAGDFARSLLNGGTLVICPDevkLDPAALYDLILKS---RITLMESTPAL 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 303 YSKLMDYHDRHFGQPhvrdfvravceEKIRLMVSGSAALPLpvleKWKgatgHTLLER----------YGMTEIGMALS- 371
Cdd:cd17650 196 IRPVMAYVYRNGLDL-----------SAMRLLIVGSDGCKA----QDF----KTLAARfgqgmriinsYGVTEATIDSTy 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 372 NPLATARLPGS----VGTPLPGVEVRIVSE--NPQKDGcpyiihaegnetetrvtpgfeeTEGELLVRGPSVFRGYWDKP 445
Cdd:cd17650 257 YEEGRDPLGDSanvpIGRPLPNTAMYVLDErlQPQPVG----------------------VAGELYIGGAGVARGYLNRP 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 446 EETKNAFTSDGW------FKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWG 518
Cdd:cd17650 315 ELTAERFVENPFapgermYRTGDLARWRaDGNVELLGRVD-HQVKIRGFRIELGEIESQLARHPAIDEAVVAVREDKGGE 393
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 519 QRVTAVVTLRegHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17650 394 ARLCAYVVAA--ATLNTAELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
73-571 |
3.80e-32 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 131.01 E-value: 3.80e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:cd17641 13 TWADYADRVRAFALGLLAL-----GVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVV 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAG-QEHVELLSPVVRKLgvplLPLPPTVYC-------------------------GAAKDPG--EEQVPERDWRDRgAM 204
Cdd:cd17641 88 IAEdEEQVDKLLEIADRI----PSVRYVIYCdprgmrkyddprlisfedvvalgraLDRRDPGlyEREVAAGKGEDV-AV 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGaTCVMLPE--------- 275
Cdd:cd17641 163 LCTTSGTTGKPKLAMLSHGNFLGHCAAYLAADPLGPGDEYVSVLPLPWIGEQMYSVGQALVCG-FIVNFPEepetmmedl 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 276 --------FSAQLVWEKFLS------SQSPRVNVFM----------AVPTIYS----KLMDYHDRHFGQPHV----RD-- 321
Cdd:cd17641 242 reigptfvLLPPRVWEGIAAdvrarmMDATPFKRFMfelgmklglrALDRGKRgrpvSLWLRLASWLADALLfrplRDrl 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 322 -FVRavceekIRLMVSGSAALPlPVLEKWKGATGHTLLERYGMTEI-GMALSNPLATARlPGSVGTPLPGVEVRIvsenp 399
Cdd:cd17641 322 gFSR------LRSAATGGAALG-PDTFRFFHAIGVPLKQLYGQTELaGAYTVHRDGDVD-PDTVGVPFPGTEVRI----- 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 400 qkdgcpyiihaegnetetrvtpgfeETEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFK-DGSYWIRGRTS 478
Cdd:cd17641 389 -------------------------DEVGEILVRSPGVFVGYYKNPEATAEDFDEDGWLHTGDAGYFKeNGHLVVIDRAK 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 479 vDIIKTG-GYKVSALEVERLLLGHPSIADVAVIGVPDvtwgQRVTAVVTLReghslsHRELKEWARGVLAPYAVPSELLL 557
Cdd:cd17641 444 -DVGTTSdGTRFSPQFIENKLKFSPYIAEAVVLGAGR----PYLTAFICID------YAIVGKWAEQRGIAFTTYTDLAS 512
|
570
....*....|....*..
gi 743747175 558 ---VEEIPRNQMGKVNK 571
Cdd:cd17641 513 rpeVYELIRKEVEKVNA 529
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
207-582 |
7.43e-32 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 130.14 E-value: 7.43e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 207 YTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGvvnkllcplWV--------GATCVMLPEFSA 278
Cdd:PLN03102 193 YTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCNG---------WTftwgtaarGGTSVCMRHVTA 263
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 QLVWEKFlssQSPRVNVFMAVPTIYSKLMD---YHDRHFGQPhvrdfvravceekIRLMVSGSAalPLPVLEKWKGATGH 355
Cdd:PLN03102 264 PEIYKNI---EMHNVTHMCCVPTVFNILLKgnsLDLSPRSGP-------------VHVLTGGSP--PPAALVKKVQRLGF 325
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 356 TLLERYGMTEIgmalSNPLATA-------RLPGSVGTPLP---GVEVRIVSEnpqkdgcpyiIHAEGNETETRVtPGFEE 425
Cdd:PLN03102 326 QVMHAYGLTEA----TGPVLFCewqdewnRLPENQQMELKarqGVSILGLAD----------VDVKNKETQESV-PRDGK 390
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 426 TEGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTAVFK-DGSYWIRGRtSVDIIKTGGYKVSALEVERLLLGHPSI 504
Cdd:PLN03102 391 TMGEIVIKGSSIMKGYLKNPKATSEAF-KHGWLNTGDVGVIHpDGHVEIKDR-SKDIIISGGENISSVEVENVLYKYPKV 468
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 505 ADVAVIGVPDVTWGQRVTAVVTLREGHS----------LSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNK--- 571
Cdd:PLN03102 469 LETAVVAMPHPTWGETPCAFVVLEKGETtkedrvdklvTRERDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKpkl 548
|
410
....*....|.
gi 743747175 572 RDLVQQLYPQD 582
Cdd:PLN03102 549 RDIAKGLVVED 559
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
205-572 |
9.23e-32 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 130.26 E-value: 9.23e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVitH----HNVWATVTglvHKWA---------WTKDDV--------IlhvlplhhVHGvvnkllcP 263
Cdd:PRK00174 250 ILYTSGSTGKPKGVL--HttggYLVYAAMT---MKYVfdykdgdvyWCTADVgwvtghsyI--------VYG-------P 309
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 264 LWVGATCVML---PEFSAQLVW----EKFlssqspRVNVFMAVPTIYSKLMDYhdrhfGQPHVRDFVRAvceeKIRLMvs 336
Cdd:PRK00174 310 LANGATTLMFegvPNYPDPGRFweviDKH------KVTIFYTAPTAIRALMKE-----GDEHPKKYDLS----SLRLL-- 372
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 337 GSAALPL-PvlEKWK------GAtghtllER------YGMTEIGMALSNPL--ATARLPGSVGTPLPGVEVRIVSEnpqk 401
Cdd:PRK00174 373 GSVGEPInP--EAWEwyykvvGG------ERcpivdtWWQTETGGIMITPLpgATPLKPGSATRPLPGIQPAVVDE---- 440
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 402 dgcpyiihaEGNETEtrvtpgfEETEGELLVRG--PSVFRGYWDKPEETKNAFTSD--GWFKTGDTAVF-KDGSYWIRGR 476
Cdd:PRK00174 441 ---------EGNPLE-------GGEGGNLVIKDpwPGMMRTIYGDHERFVKTYFSTfkGMYFTGDGARRdEDGYYWITGR 504
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 477 TSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLS---HRELKEWARGVLAPYAVPS 553
Cdd:PRK00174 505 VD-DVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIKGQGIYAFVTLKGGEEPSdelRKELRNWVRKEIGPIAKPD 583
|
410
....*....|....*....
gi 743747175 554 ELLLVEEIPRNQMGKVNKR 572
Cdd:PRK00174 584 VIQFAPGLPKTRSGKIMRR 602
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
73-574 |
1.71e-31 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 127.55 E-value: 1.71e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAReicRLRACSDGDlrEERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:cd17644 27 TYEELNTKANQLAH---YLQSLGVKS--ESLVGICVERSLEMIIGLLAILKAGGAYVPLDPNYPQERLTYILEDAQISVL 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHVellspvvrklgvpllplpptvycgaakdpgeeqvperdwrdrgAMIIYTSGTTGRPKGVVITHHNVWATVTGL 232
Cdd:cd17644 102 LTQPENL-------------------------------------------AYVIYTSGSTGKPKGVMIEHQSLVNLSHGL 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 233 VHKWAWTKDDVILHVLPLhhVHGVVNKLLCPLWV-GATCVMLPE---FSAQLVWEKflsSQSPRVNVFMAVPTIYSKLMD 308
Cdd:cd17644 139 IKEYGITSSDRVLQFASI--AFDVAAEEIYVTLLsGATLVLRPEemrSSLEDFVQY---IQQWQLTVLSLPPAYWHLLVL 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 309 yhdrhfgqphvrDFVRAVCE--EKIRLMVSGSAALPLPVLEKWKGATGH--TLLERYGMTEIGMA-----LSNPLATARL 379
Cdd:cd17644 214 ------------ELLLSTIDlpSSLRLVIVGGEAVQPELVRQWQKNVGNfiQLINVYGPTEATIAatvcrLTQLTERNIT 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 380 PGSVGTPLPGVEVRIVSENPQKdgcpyiihaegnetetrVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDGW-- 457
Cdd:cd17644 282 SVPIGRPIANTQVYILDENLQP-----------------VPVG---VPGELHIGGVGLARGYLNRPELTAEKFISHPFns 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 458 ------FKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREG 530
Cdd:cd17644 342 seserlYKTGDLARYlPDGNIEYLGRID-NQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVREDQPGNKRLVAYIVPHYE 420
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 743747175 531 HSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17644 421 ESPSTVELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
247-586 |
3.63e-31 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 126.26 E-value: 3.63e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 247 VLPLHHVHGVVnKLLCPLWVGATCVMLPefsaqlvWEKFLSSQSPRVNV---FMA-VPTIYSKLMDYHDRHFGQphvrdf 322
Cdd:PRK07445 166 VLPLYHVSGLM-QFMRSFLTGGKLVILP-------YKRLKSGQELPPNPsdfFLSlVPTQLQRLLQLRPQWLAQ------ 231
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 323 VRAVceekirlMVSGSAALPlpvlekwkgatghTLLER-----------YGMTEIGMALSNPLATARLPG--SVGTPLPG 389
Cdd:PRK07445 232 FRTI-------LLGGAPAWP-------------SLLEQarqlqlrlaptYGMTETASQIATLKPDDFLAGnnSSGQVLPH 291
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 390 VEVRIVSenpqkdgcpyiiHAEGNETetrvtpgfeetegellVRGPSVFRGYWdkPeetkNAFTSDGWFKTGDTAVF-KD 468
Cdd:PRK07445 292 AQITIPA------------NQTGNIT----------------IQAQSLALGYY--P----QILDSQGIFETDDLGYLdAQ 337
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 469 GSYWIRGRTSVDIIkTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGhSLSHRELKEWARGVLAP 548
Cdd:PRK07445 338 GYLHILGRNSQKII-TGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDP-SISLEELKTAIKDQLSP 415
|
330 340 350
....*....|....*....|....*....|....*...
gi 743747175 549 YAVPSELLLVEEIPRNQMGKVNkRDLVQQLYPQDRGTP 586
Cdd:PRK07445 416 FKQPKHWIPVPQLPRNPQGKIN-RQQLQQIAVQRLGLP 452
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
185-569 |
3.64e-31 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 128.08 E-value: 3.64e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 185 AKDPGEEQvPERDWRDRGAMIIYTSGTTGRPKGVVITH--HNVWATvTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLC 262
Cdd:cd17634 218 AKASPEHQ-PEAMNAEDPLFILYTSGTTGKPKGVLHTTggYLVYAA-TTMKYVFDYGPGDIYWCTADVGWVTGHSYLLYG 295
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 263 PLWVGATCVML---PEF-SAQLVWEKFLSSQsprVNVFMAVPTIYSKLM----DYHDRHfgqphvrdfvravceEKIRLM 334
Cdd:cd17634 296 PLACGATTLLYegvPNWpTPARMWQVVDKHG---VNILYTAPTAIRALMaagdDAIEGT---------------DRSSLR 357
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 335 VSGSAALPL-PVLEKWK----GATGHTLLERYGMTEIGMALSNPL--ATARLPGSVGTPLPGVEVRIVSE--NPQKDGcp 405
Cdd:cd17634 358 ILGSVGEPInPEAYEWYwkkiGKEKCPVVDTWWQTETGGFMITPLpgAIELKAGSATRPVFGVQPAVVDNegHPQPGG-- 435
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 406 yiihAEGNETETRVTPGFEETegellvrgpsvfrGYWDKPEETKNAF-TSDGWFKTGDTA-VFKDGSYWIRGRtSVDIIK 483
Cdd:cd17634 436 ----TEGNLVITDPWPGQTRT-------------LFGDHERFEQTYFsTFKGMYFSGDGArRDEDGYYWITGR-SDDVIN 497
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 484 TGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLS---HRELKEWARGVLAPYAVPSELLLVEE 560
Cdd:cd17634 498 VAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSpelYAELRNWVRKEIGPLATPDVVHWVDS 577
|
....*....
gi 743747175 561 IPRNQMGKV 569
Cdd:cd17634 578 LPKTRSGKI 586
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
60-576 |
3.97e-31 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 125.88 E-value: 3.97e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 60 ADQHGSHI----PAGSHTYRDLYSRSLSLAREICRLRACSdGDlreeRVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKH 135
Cdd:cd17653 7 AAAHPDAVavesLGGSLTYGELDAASNALANRLLQLGVVP-GD----VVPLLSDRSLEMLVAILAILKAGAAYVPLDAKL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 136 PQAELEYFIQDSRSSVVlagqehvellspvvrklgvpllplpptVYCGAAKDPgeeqvperdwrdrgAMIIYTSGTTGRP 215
Cdd:cd17653 82 PSARIQAILRTSGATLL---------------------------LTTDSPDDL--------------AYIIFTSGSTGIP 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 216 KGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVvNKLLCPLWVGATCVmLPEFSAQLvwekflSSQSPRVNV 295
Cdd:cd17653 121 KGVMVPHRGVLNYVSQPPARLDVGPGSRVAQVLSIAFDACI-GEIFSTLCNGGTLV-LADPSDPF------AHVARTVDA 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 296 FMAVPTIYSKLmdyhdrhfgqpHVRDFVRavceekIRLMVSGSAALPLPVLEKWKGatGHTLLERYGMTEIGMALSNPLA 375
Cdd:cd17653 193 LMSTPSILSTL-----------SPQDFPN------LKTIFLGGEAVPPSLLDRWSP--GRRLYNAYGPTECTISSTMTEL 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 376 TARLPGSVGTPLPGVEVRIVSENPQkdgcpyiihaegnetetrvtPGFEETEGELLVRGPSVFRGYWDKPEETKNAFTSD 455
Cdd:cd17653 254 LPGQPVTIGKPIPNSTCYILDADLQ--------------------PVPEGVVGEICISGVQVARGYLGNPALTASKFVPD 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 456 GW------FKTGDTAVF-KDGSYWIRGRTSVDIiKTGGYKVSALEVER-LLLGHPSIADVAVIGVpdvtwGQRVTAVVTl 527
Cdd:cd17653 314 PFwpgsrmYRTGDYGRWtEDGGLEFLGREDNQV-KVRGFRINLEEIEEvVLQSQPEVTQAAAIVV-----NGRLVAFVT- 386
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 743747175 528 reGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQ 576
Cdd:cd17653 387 --PETVDVDGLRSELAKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
73-574 |
6.53e-31 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 129.13 E-value: 6.53e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PRK12467 1601 TYGELNRRANRLAHRLIAL-----GVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELL 1675
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAgQEHVELLSPV---VRKLGVPLLPLPPTVYcgAAKDPGEEQVPerdwrDRGAMIIYTSGTTGRPKGVVITHHNVWATV 229
Cdd:PRK12467 1676 LT-QSHLQARLPLpdgLRSLVLDQEDDWLEGY--SDSNPAVNLAP-----QNLAYVIYTSGSTGRPKGAGNRHGALVNRL 1747
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 230 TGLVHKWAWTKDDVILHVLPlHHVHGVVNKLLCPLWVGATCVMLPeFSAQLVWEKFLSS-QSPRVNVFMAVPTIYSKLMD 308
Cdd:PRK12467 1748 CATQEAYQLSAADVVLQFTS-FAFDVSVWELFWPLINGARLVIAP-PGAHRDPEQLIQLiERQQVTTLHFVPSMLQQLLQ 1825
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 309 yHDRHFGQPhvrdfvravceEKIRLMVSGSAALPLPVLEKWKGATGHT-LLERYGMTEIGM-----ALSNPLATARLPGS 382
Cdd:PRK12467 1826 -MDEQVEHP-----------LSLRRVVCGGEALEVEALRPWLERLPDTgLFNLYGPTETAVdvthwTCRRKDLEGRDSVP 1893
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 383 VGTPLPGVEVRIVSE--NPQKDGCPyiihaegnetetrvtpgfeeteGELLVRGPSVFRGYWDKPEETKNAFTSDGW--- 457
Cdd:PRK12467 1894 IGQPIANLSTYILDAslNPVPIGVA----------------------GELYLGGVGLARGYLNRPALTAERFVADPFgtv 1951
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 458 ----FKTGDTAVFK-DGSYWIRGRtsVD-IIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREG- 530
Cdd:PRK12467 1952 gsrlYRTGDLARYRaDGVIEYLGR--IDhQVKIRGFRIELGEIEARLREQGGVREAVVIAQDGANGKQLVAYVVPTDPGl 2029
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 743747175 531 ------HSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK12467 2030 vdddeaQVALRAILKNHLKASLPEYMVPAHLVFLARMPLTPNGKLDRKAL 2079
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
22-574 |
3.10e-30 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 127.38 E-value: 3.10e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 22 APW-RPGGRSPLHTGPAARgnrsapVFTRALAFGDRVAL--ADQHGShipagshtYRDLYSRSLSLAReicRLRACSDGD 98
Cdd:PRK12316 1990 ADWdRTPEAYPRGPGVHQR------IAEQAARAPEAIAVvfGDQHLS--------YAELDSRANRLAH---RLRARGVGP 2052
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 99 lrEERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAgQEHVELLSPVVRKLGVPLLPLPP 178
Cdd:PRK12316 2053 --EVRVAIAAERSFELVVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLLT-QRHLLERLPLPAGVARLPLDRDA 2129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 179 TVYCGAAKDPGEEQVPerdwrDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLhHVHGVVN 258
Cdd:PRK12316 2130 EWADYPDTAPAVQLAG-----ENLAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGERYELSPADCELQFMSF-SFDGAHE 2203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 259 KLLCPLWVGATCVMLPefsAQLVWEKFLSSQSPRVNVFMAV-PTIYSKLMDYHDRHFGQPhvrdfvravceEKIRLMVSG 337
Cdd:PRK12316 2204 QWFHPLLNGARVLIRD---DELWDPEQLYDEMERHGVTILDfPPVYLQQLAEHAERDGRP-----------PAVRVYCFG 2269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 338 SAALPLPVLEKWKGAT-GHTLLERYGMTEIGMALS-------NPLATARLPgsVGTPLPGVEVrivsenpqkdgcpYIIH 409
Cdd:PRK12316 2270 GEAVPAASLRLAWEALrPVYLFNGYGPTEAVVTPLlwkcrpqDPCGAAYVP--IGRALGNRRA-------------YILD 2334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 410 AEGNETETRVTpgfeeteGELLVRGPSVFRGYWDKPEETKNAFTSDGW-------FKTGDTAVFK-DGSYWIRGRtsVD- 480
Cdd:PRK12316 2335 ADLNLLAPGMA-------GELYLGGEGLARGYLNRPGLTAERFVPDPFsasgerlYRTGDLARYRaDGVVEYLGR--IDh 2405
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 481 IIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTwGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEE 560
Cdd:PRK12316 2406 QVKIRGFRIELGEIEARLQAHPAVREAVVVAQDGAS-GKQLVAYVVPDDAAEDLLAELRAWLAARLPAYMVPAHWVVLER 2484
|
570
....*....|....
gi 743747175 561 IPRNQMGKVNKRDL 574
Cdd:PRK12316 2485 LPLNPNGKLDRKAL 2498
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
49-578 |
3.72e-30 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 123.81 E-value: 3.72e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALadqhgsHIPAGSHTYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:cd05918 8 RARSQPDAPAV------CAWDGSLTYAELDRLSSRLAHHLRSL-----GVGPGVFVPLCFEKSKWAVVAMLAVLKAGGAF 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLYRKHPQAELEYFIQDSRSSVVLAGQEHvellspvvrklgvpllplpptvycgaakDPgeeqvperdwrdrgAMIIYT 208
Cdd:cd05918 77 VPLDPSHPLQRLQEILQDTGAKVVLTSSPS----------------------------DA--------------AYVIFT 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 209 SGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLplHHVHGV-VNKLLCPLWVGAT-CVM--------LPEFSA 278
Cdd:cd05918 115 SGSTGKPKGVVIEHRALSTSALAHGRALGLTSESRVLQFA--SYTFDVsILEIFTTLAAGGClCIPseedrlndLAGFIN 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 QLvwekflssqspRVNVFMAVPTIySKLMDYHDrhfgQPHVRdfvravceekirLMVSGSAALPLPVLEKWkgATGHTLL 358
Cdd:cd05918 193 RL-----------RVTWAFLTPSV-ARLLDPED----VPSLR------------TLVLGGEALTQSDVDTW--ADRVRLI 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 359 ERYGMTE--IGMALSNPLATARlPGSVGTPLPGVeVRIVSENpqkdgcpyiihaegneTETRVTP-GfeeTEGELLVRGP 435
Cdd:cd05918 243 NAYGPAEctIAATVSPVVPSTD-PRNIGRPLGAT-CWVVDPD----------------NHDRLVPiG---AVGELLIEGP 301
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 436 SVFRGYWDKPEETKNAFTSD-GW------------FKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGH 501
Cdd:cd05918 302 ILARGYLNDPEKTAAAFIEDpAWlkqegsgrgrrlYRTGDLVRYnPDGSLEYVGRKD-TQVKIRGQRVELGEIEHHLRQS 380
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 502 PSIADVAVIGV---PDVTWGQRVTAVVTLREGHSLSH-----------------RELKEWARGVLAPYAVPSELLLVEEI 561
Cdd:cd05918 381 LPGAKEVVVEVvkpKDGSSSPQLVAFVVLDGSSSGSGdgdslflepsdefralvAELRSKLRQRLPSYMVPSVFLPLSHL 460
|
570
....*....|....*..
gi 743747175 562 PRNQMGKVNKRDLVQQL 578
Cdd:cd05918 461 PLTASGKIDRRALRELA 477
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
200-577 |
6.60e-30 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 125.14 E-value: 6.60e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 200 DRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHK-WAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLP---- 274
Cdd:PRK06060 145 DALAYATYTSGTTGPPKAAIHRHADPLTFVDAMCRKaLRLTPEDTGLCSARMYFAYGLGNSVWFPLATGGSAVINSapvt 224
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 275 EFSAQLVWEKFLSSqsprvnVFMAVPTIYSKLMDYHDRhfgqphvrDFVRAVceekiRLMVSGSAALPLPVLEKW-KGAT 353
Cdd:PRK06060 225 PEAAAILSARFGPS------VLYGVPNFFARVIDSCSP--------DSFRSL-----RCVVSAGEALELGLAERLmEFFG 285
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 354 GHTLLERYGMTEIGMA-LSNPLATARlPGSVGTPLPGVEVRIVSENPQKDGcpyiihaegnetetrvtPGfeeTEGELLV 432
Cdd:PRK06060 286 GIPILDGIGSTEVGQTfVSNRVDEWR-LGTLGRVLPPYEIRVVAPDGTTAG-----------------PG---VEGDLWV 344
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 433 RGPSVFRGYWDKPEETknaFTSDGWFKTGDTaVFKDGSYWIR-GRTSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIG 511
Cdd:PRK06060 345 RGPAIAKGYWNRPDSP---VANEGWLDTRDR-VCIDSDGWVTyRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVA 420
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 743747175 512 VPDVTWGQRVTAVVTLREGHSLSHRELKEWARGV---LAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQ 577
Cdd:PRK06060 421 VRESTGASTLQAFLVATSGATIDGSVMRDLHRGLlnrLSAFKVPHRFAVVDRLPRTPNGKLVRGALRKQ 489
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
104-577 |
1.11e-29 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 123.21 E-value: 1.11e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 104 VSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELLS----PVVRKLGVPLLPlppt 179
Cdd:PRK13388 55 VGVLLGNTPEMLFWLAAAALGGYVLVGLNTTRRGAALAADIRRADCQLLVTDAEHRPLLDgldlPGVRVLDVDTPA---- 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 180 vYCGAAKDPGEEqVPERDW-RDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVN 258
Cdd:PRK13388 131 -YAELVAAAGAL-TPHREVdAMDPFMLIFTSGTTGAPKAVRCSHGRLAFAGRALTERFGLTRDDVCYVSMPLFHSNAVMA 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 259 KLLCPLWVGATCVMLPEFSAQlvweKFLssqsPRVNVFMAVPTIY-SKLMDY------HDRHFGQPHVRDFvravceeki 331
Cdd:PRK13388 209 GWAPAVASGAAVALPAKFSAS----GFL----DDVRRYGATYFNYvGKPLAYilatpeRPDDADNPLRVAF--------- 271
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 332 rlmvsGSAALPLPVlEKWKGATGHTLLERYGMTEIGMALSNPLATArlPGSVGTPLPGVEvrIVSENPQKDGCPYIIHAE 411
Cdd:PRK13388 272 -----GNEASPRDI-AEFSRRFGCQVEDGYGSSEGAVIVVREPGTP--PGSIGRGAPGVA--IYNPETLTECAVARFDAH 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 412 GnetetRVTpGFEETEGELLVR-GPSVFRGYWDKPEETKNAFtSDGWFKTGDTA-VFKDGSYWIRGRTSvDIIKTGGYKV 489
Cdd:PRK13388 342 G-----ALL-NADEAIGELVNTaGAGFFEGYYNNPEATAERM-RHGMYWSGDLAyRDADGWIYFAGRTA-DWMRVDGENL 413
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 490 SALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEW--ARGVLAPYAVPSELLLVEEIPRNQMG 567
Cdd:PRK13388 414 SAAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGATFDPDAFAAFlaAQPDLGTKAWPRYVRIAADLPSTATN 493
|
490
....*....|
gi 743747175 568 KVNKRDLVQQ 577
Cdd:PRK13388 494 KVLKRELIAQ 503
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
207-578 |
1.48e-29 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 122.78 E-value: 1.48e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 207 YTSGTTGRPKGVVITHHNVwatVTGLVHKWAWTKDDVI-----LHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLV 281
Cdd:PLN02330 191 FSSGTTGISKGVMLTHRNL---VANLCSSLFSVGPEMIgqvvtLGLIPFFHIYGITGICCATLRNKGKVVVMSRFELRTF 267
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 282 WEKFLSSQsprVNVFMAVPTIYSKLMDyhdrhfgQPHVRDFvrAVCEEKIRLMVSGSAALPLPVLEKWKGA-TGHTLLER 360
Cdd:PLN02330 268 LNALITQE---VSFAPIVPPIILNLVK-------NPIVEEF--DLSKLKLQAIMTAAAPLAPELLTAFEAKfPGVQVQEA 335
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 361 YGMTE---IGMALSNPLATARLP--GSVGTPLPGVEVRIVseNPQKDgcpyiihaegnetetRVTPgfEETEGELLVRGP 435
Cdd:PLN02330 336 YGLTEhscITLTHGDPEKGHGIAkkNSVGFILPNLEVKFI--DPDTG---------------RSLP--KNTPGELCVRSQ 396
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 436 SVFRGYWDKPEETKNAFTSDGWFKTGDTA-VFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPD 514
Cdd:PLN02330 397 CVMQGYYNNKEETDRTIDEDGWLHTGDIGyIDDDGDIFIVDRIK-ELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPD 475
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 743747175 515 VTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PLN02330 476 EEAGEIPAACVVINPKAKESEEDILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKEKM 539
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
73-571 |
2.23e-29 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 122.04 E-value: 2.23e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREIcrlracSDGDLRE-ERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSV 151
Cdd:PRK13390 26 SYRQLDDDSAALARVL------YDAGLRTgDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAPEADYIVGDSGARV 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 152 VLAGQEhvelLSPVVRKLGVPLLPLPP-----------TVYCGAAKDPGEEQvperdwrDRGAMIIYTSGTTGRPKG--- 217
Cdd:PRK13390 100 LVASAA----LDGLAAKVGADLPLRLSfggeidgfgsfEAALAGAGPRLTEQ-------PCGAVMLYSSGTTGFPKGiqp 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 218 ------VVITHHNVWATVTGLvhkWAWTKDDVILHVLPLHHVH-----GVVNKLlcplwvGATCVMLPEFSAQLVW---E 283
Cdd:PRK13390 169 dlpgrdVDAPGDPIVAIARAF---YDISESDIYYSSAPIYHAAplrwcSMVHAL------GGTVVLAKRFDAQATLghvE 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 KFlssqspRVNVFMAVPTIYSKLMDYHDRhfgqphvrdfVRAVCE-EKIRLMVSGSAALPLPVLEKWKGATGHTLLERYG 362
Cdd:PRK13390 240 RY------RITVTQMVPTMFVRLLKLDAD----------VRTRYDvSSLRAVIHAAAPCPVDVKHAMIDWLGPIVYEYYS 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 363 MTEI-GMALSNPLATARLPGSVGTPLPGVevrivsenpqkdgcPYIIHAEGNETET-RV-TPGFEetegellvRGPSVFR 439
Cdd:PRK13390 304 STEAhGMTFIDSPDWLAHPGSVGRSVLGD--------------LHICDDDGNELPAgRIgTVYFE--------RDRLPFR 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 440 gYWDKPEETKNAF--TSDGWFKTGDTA-VFKDGSYWIRGRTSVDIIkTGGYKVSALEVERLLLGHPSIADVAVIGVPDVT 516
Cdd:PRK13390 362 -YLNDPEKTAAAQhpAHPFWTTVGDLGsVDEDGYLYLADRKSFMII-SGGVNIYPQETENALTMHPAVHDVAVIGVPDPE 439
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 743747175 517 WGQRVTAVVTLREGHSLSH---RELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNK 571
Cdd:PRK13390 440 MGEQVKAVIQLVEGIRGSDelaRELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVK 497
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
73-584 |
2.36e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 124.68 E-value: 2.36e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAReicRLRACSDGDlrEERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PRK12316 538 DYAELNRRANRLAH---ALIERGVGP--DVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYMLEDSGVQLL 612
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAgQEHVELLSPVVRKLGVPLLPLPPTVYCG-AAKDPGEEQVPERDwrdrgAMIIYTSGTTGRPKGVVITHHNVWATVTG 231
Cdd:PRK12316 613 LS-QSHLGRKLPLAAGVQVLDLDRPAAWLEGySEENPGTELNPENL-----AYVIYTSGSTGKPKGAGNRHRALSNRLCW 686
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 232 LVHKWAWTKDDVILHVLPLHHVHGVVnKLLCPLWVGATCVMLPE---FSAQLVWEkfLSSQSpRVNVFMAVPTIYSKLMd 308
Cdd:PRK12316 687 MQQAYGLGVGDTVLQKTPFSFDVSVW-EFFWPLMSGARLVVAAPgdhRDPAKLVE--LINRE-GVDTLHFVPSMLQAFL- 761
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 309 yhdrHFGQPhvrdfvrAVCEEkIRLMVSGSAALPLPVLEK--WKGATGHtLLERYGMTEIGMALSNplATARLPG----S 382
Cdd:PRK12316 762 ----QDEDV-------ASCTS-LRRIVCSGEALPADAQEQvfAKLPQAG-LYNLYGPTEAAIDVTH--WTCVEEGgdsvP 826
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 383 VGTPLPGVevrivsenpqkdGCpYIIHAEGNETETRVTpgfeeteGELLVRGPSVFRGYWDKPEETKNAFTSDGW----- 457
Cdd:PRK12316 827 IGRPIANL------------AC-YILDANLEPVPVGVL-------GELYLAGRGLARGYHGRPGLTAERFVPSPFvager 886
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 458 -FKTGDTAVFK-DGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPdvtwGQRVTAVVTLREGHSLSH 535
Cdd:PRK12316 887 mYRTGDLARYRaDGVIEYAGRID-HQVKLRGLRIELGEIEARLLEHPWVREAAVLAVD----GKQLVGYVVLESEGGDWR 961
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 536 RELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL-------VQQLYPQDRG 584
Cdd:PRK12316 962 EALKAHLAASLPEYMVPAQWLALERLPLTPNGKLDRKALpapeasvAQQGYVAPRN 1017
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
49-576 |
2.88e-28 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 121.22 E-value: 2.88e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVALAdqhgshIPAGSHTYRDLYSRSLSLAReicRLRACSDGDlrEERVSFLCSNDVSYVVAQWASWMSGGTA 128
Cdd:PRK12316 4560 RARMTPDAVAVV------FDEEKLTYAELNRRANRLAH---ALIARGVGP--EVLVGIAMERSAEMMVGLLAVLKAGGAY 4628
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 129 VPLYRKHPQAELEYFIQDSRSSVVLAgQEHVELLSPVVRKLGVPLLPlpptvycgaakdpgeeqvPERDWRDRG------ 202
Cdd:PRK12316 4629 VPLDPEYPRERLAYMMEDSGAALLLT-QSHLLQRLPIPDGLASLALD------------------RDEDWEGFPahdpav 4689
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 -------AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLhHVHGVVNKLLCPLWVGATCVM--- 272
Cdd:PRK12316 4690 rlhpdnlAYVIYTSGSTGRPKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSF-SFDGSHEGLYHPLINGASVVIrdd 4768
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 273 ---LPEFSAQLVWEKflssqspRVNVFMAVPTIYSKLMDyHDRHFGQPhvrdfvravceEKIRLMVSGSAALPLPVLEKW 349
Cdd:PRK12316 4769 slwDPERLYAEIHEH-------RVTVLVFPPVYLQQLAE-HAERDGEP-----------PSLRVYCFGGEAVAQASYDLA 4829
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 350 KGATGHT-LLERYGMTEigmALSNPLATARLPGS--------VGTPLPGVEVRI--VSENPQKDGcpyiihaegnetetr 418
Cdd:PRK12316 4830 WRALKPVyLFNGYGPTE---TTVTVLLWKARDGDacgaaympIGTPLGNRSGYVldGQLNPLPVG--------------- 4891
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 419 vtpgfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDGW-------FKTGDTAVFK-DGSYWIRGRtsVD-IIKTGGYKV 489
Cdd:PRK12316 4892 -------VAGELYLGGEGVARGYLERPALTAERFVPDPFgapggrlYRTGDLARYRaDGVIDYLGR--VDhQVKIRGFRI 4962
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 490 SALEVERLLLGHPSIADVAVIGVPDVTWGQRV-------TAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIP 562
Cdd:PRK12316 4963 ELGEIEARLREHPAVREAVVIAQEGAVGKQLVgyvvpqdPALADADEAQAELRDELKAALRERLPEYMVPAHLVFLARMP 5042
|
570
....*....|....
gi 743747175 563 RNQMGKVNKRDLVQ 576
Cdd:PRK12316 5043 LTPNGKLDRKALPQ 5056
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
73-574 |
3.15e-28 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 120.83 E-value: 3.15e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAReicRLRACSDGDLREERVSFLCSNDVsyVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PRK12316 3084 SYAELNRRANRLAH---RLIERGVGPDVLVGVAVERSLEM--VVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLL 3158
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAgQEHVEL-LSPVVRKLGVPLLplpptvycgaAKDPGEEQVPERDWRDRGAMIIYTSGTTGRPKGVVITHHNVWATVTG 231
Cdd:PRK12316 3159 LS-QSHLRLpLAQGVQVLDLDRG----------DENYAEANPAIRTMPENLAYVIYTSGSTGKPKGVGIRHSALSNHLCW 3227
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 232 LVHKWAWTKDDVILHVLPLhHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWEKFLSSQSPRVNVFMAVPTIYSKLmdyhd 311
Cdd:PRK12316 3228 MQQAYGLGVGDRVLQFTTF-SFDVFVEELFWPLMSGARVVLAGPEDWRDPALLVELINSEGVDVLHAYPSMLQAF----- 3301
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 312 rhFGQPHVRDFVravceeKIRLMVSGSAALPLPVLEKWkgATGHTLLERYGMTEIGMALSNPLATARLPGS--VGTPLPG 389
Cdd:PRK12316 3302 --LEEEDAHRCT------SLKRIVCGGEALPADLQQQV--FAGLPLYNLYGPTEATITVTHWQCVEEGKDAvpIGRPIAN 3371
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 390 VEVRIVSENpqkdgcpyiihaegnetetrVTPGFEETEGELLVRGPSVFRGYWDKPEETKNAFTSDGW------FKTGDT 463
Cdd:PRK12316 3372 RACYILDGS--------------------LEPVPVGALGELYLGGEGLARGYHNRPGLTAERFVPDPFvpgerlYRTGDL 3431
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 464 AVFK-DGSYWIRGRTSVDiIKTGGYKVSALEVERLLLGHPSIADVAVIGVPdvtwGQRVTAVVTLREGHSLSHRELKEWA 542
Cdd:PRK12316 3432 ARYRaDGVIEYIGRVDHQ-VKIRGFRIELGEIEARLLEHPWVREAVVLAVD----GRQLVAYVVPEDEAGDLREALKAHL 3506
|
490 500 510
....*....|....*....|....*....|..
gi 743747175 543 RGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK12316 3507 KASLPEYMVPAHLLFLERMPLTPNGKLDRKAL 3538
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
203-462 |
4.33e-28 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 119.05 E-value: 4.33e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCpLWVGatcVMLPEFSAQLVw 282
Cdd:PLN02736 224 ATICYTSGTTGTPKGVVLTHGNLIANVAGSSLSTKFYPSDVHISYLPLAHIYERVNQIVM-LHYG---VAVGFYQGDNL- 298
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 283 eKFLSSQSP-RVNVFMAVPTIYSKLMD-----------YHDRHF-------------GQPH-------VRDFVRAVCEEK 330
Cdd:PLN02736 299 -KLMDDLAAlRPTIFCSVPRLYNRIYDgitnavkesggLKERLFnaaynakkqalenGKNPspmwdrlVFNKIKAKLGGR 377
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 331 IRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIGMALSNPLATARLPGSVGTPLPGVEVRIVS---ENPQKDGCPYi 407
Cdd:PLN02736 378 VRFMSSGASPLSPDVMEFLRICFGGRVLEGYGMTETSCVISGMDEGDNLSGHVGSPNPACEVKLVDvpeMNYTSEDQPY- 456
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*
gi 743747175 408 ihaegnetetrvtpgfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGD 462
Cdd:PLN02736 457 ------------------PRGEICVRGPIIFKGYYKDEVQTREVIDEDGWLHTGD 493
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
194-574 |
1.22e-27 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 117.25 E-value: 1.22e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 194 PERDWRDrgAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGvvnklLCPLWVGA----T 269
Cdd:PLN02479 191 PADEWQS--IALGYTSGTTASPKGVVLHHRGAYLMALSNALIWGMNEGAVYLWTLPMFHCNG-----WCFTWTLAalcgT 263
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 270 CVMLPEFSAQLVWEKFLSSQsprVNVFMAVPTIYSKLMDY--HDRHFGQPHVrdfvravceekIRLMVSGSAALP--LPV 345
Cdd:PLN02479 264 NICLRQVTAKAIYSAIANYG---VTHFCAAPVVLNTIVNApkSETILPLPRV-----------VHVMTAGAAPPPsvLFA 329
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 346 LEKWKGATGHT--LLERYGMTEIGMAL----SNPLAT-ARLPGSVGTPLPGVEVRIVSEnpQKDGCPyiIHAEGnetetr 418
Cdd:PLN02479 330 MSEKGFRVTHTygLSETYGPSTVCAWKpewdSLPPEEqARLNARQGVRYIGLEGLDVVD--TKTMKP--VPADG------ 399
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 419 vtpgfeETEGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTAV-FKDGSYWIRGRtSVDIIKTGGYKVSALEVERL 497
Cdd:PLN02479 400 ------KTMGEIVMRGNMVMKGYLKNPKANEEAF-ANGWFHSGDLGVkHPDGYIEIKDR-SKDIIISGGENISSLEVENV 471
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 498 LLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSH-----RELKEWARGVLAPYAVPSELLLvEEIPRNQMGKVNKR 572
Cdd:PLN02479 472 VYTHPAVLEASVVARPDERWGESPCAFVTLKPGVDKSDeaalaEDIMKFCRERLPAYWVPKSVVF-GPLPKTATGKIQKH 550
|
..
gi 743747175 573 DL 574
Cdd:PLN02479 551 VL 552
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
205-574 |
2.87e-27 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 116.65 E-value: 2.87e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVV--ITHHNV---WA--TVTGLV--HKWaWTKDDVILHVLPLHHVHGvvnkllcPLWVGATCVML-- 273
Cdd:cd05967 235 ILYTSGTTGKPKGVVrdNGGHAValnWSmrNIYGIKpgDVW-WAASDVGWVVGHSYIVYG-------PLLHGATTVLYeg 306
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 274 -----PEFSAqlvWEKFLSSQspRVNVFMAVPTIYSKLmdyhdRHFgQPHVrDFVRAVCEEKIRLMVSGSAALPLPVLEK 348
Cdd:cd05967 307 kpvgtPDPGA---FWRVIEKY--QVNALFTAPTAIRAI-----RKE-DPDG-KYIKKYDLSSLRTLFLAGERLDPPTLEW 374
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 349 WKGATGHTLLERYGMTEIGMALS-NPLATARLP---GSVGTPLPGVEVRIVSENpqkdgcpyiihaeGNETETRvtpgfe 424
Cdd:cd05967 375 AENTLGVPVIDHWWQTETGWPITaNPVGLEPLPikaGSPGKPVPGYQVQVLDED-------------GEPVGPN------ 435
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 425 eTEGELLVRGP---SVFRGYWDKPEETKNAF--TSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLL 498
Cdd:cd05967 436 -ELGNIVIKLPlppGCLLTLWKNDERFKKLYlsKFPGYYDTGDAGYKdEDGYLFIMGRTD-DVINVAGHRLSTGEMEESV 513
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 499 LGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLS----HRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05967 514 LSHPAVAECAVVGVRDELKGQVPLGLVVLKEGVKITaeelEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTL 593
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
69-577 |
4.17e-27 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 115.47 E-value: 4.17e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 69 AGSHTYRDLYSRSLSLAREICRLRACSDGDLreerVSFLCSNDVSYVVAqWASWMSGGTAVPLYRKHPQAE-LEYFIQDS 147
Cdd:cd05938 3 GETYTYRDVDRRSNQAARALLAHAGLRPGDT----VALLLGNEPAFLWI-WLGLAKLGCPVAFLNTNIRSKsLLHCFRCC 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 148 RSSVVLAG---QEHVELLSPVVRKLGVPLLPLPPTVYC------GAAKDPGEEQVPERDWRDR-----GAMIIYTSGTTG 213
Cdd:cd05938 78 GAKVLVVApelQEAVEEVLPALRADGVSVWYLSHTSNTegvislLDKVDAASDEPVPASLRAHvtiksPALYIYTSGTTG 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 214 RPKGVVITHHNVWAtVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVWE---------- 283
Cdd:cd05938 158 LPKAARISHLRVLQ-CSGFLSLCGVTADDVIYITLPLYHSSGFLLGIGGCIELGATCVLKPKFSASQFWDdcrkhnvtvi 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 -------KFLSSQSPRVN-----VFMAV-----PTIYSKLMdyhdRHFGQPHVRDFvravceekirlmvsgsaalplpvl 346
Cdd:cd05938 237 qyigellRYLCNQPQSPNdrdhkVRLAIgnglrADVWREFL----RRFGPIRIREF------------------------ 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 347 ekwkgatghtllerYGMTEIGMALSNplaTARLPGSVG---------TPLPGVEVRIVSENPQKDGCPYIIHAEGNETET 417
Cdd:cd05938 289 --------------YGSTEGNIGFFN---YTGKIGAVGrvsylykllFPFELIKFDVEKEEPVRDAQGFCIPVAKGEPGL 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 418 RVTPgfeetegellVRGPSVFRGYWDKPEET-----KNAFTS-DGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVS 490
Cdd:cd05938 352 LVAK----------ITQQSPFLGYAGDKEQTekkllRDVFKKgDVYFNTGDLLVQdQQNFLYFHDRVG-DTFRWKGENVA 420
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 491 ALEVERLLLGHPSIADVAVIGVPDVTWGQRV-TAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKV 569
Cdd:cd05938 421 TTEVADVLGLLDFLQEVNVYGVTVPGHEGRIgMAAVKLKPGHEFDGKKLYQHVREYLPAYARPRFLRIQDSLEITGTFKQ 500
|
....*...
gi 743747175 570 NKRDLVQQ 577
Cdd:cd05938 501 QKVRLVEE 508
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
55-574 |
9.99e-26 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 110.34 E-value: 9.99e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADQhgshipAGSHTYRDLYSRSLSLAReicRLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRK 134
Cdd:cd17645 13 DHVAVVDR------GQSLTYKQLNEKANQLAR---HLRGK--GVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPD 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 135 HPQAELEYFIQDSRSSVVLagqehvellspvvrklgvpllplpptvycgaaKDPGEEqvperdwrdrgAMIIYTSGTTGR 214
Cdd:cd17645 82 YPGERIAYMLADSSAKILL--------------------------------TNPDDL-----------AYVIYTSGSTGL 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 215 PKGVVITHHNvwatvtgLVHKWAWTKDdvilhvlplhhVHGVVNKLLCPLWVGATcvmlpeFSAQlVWEKFlssqsPRVN 294
Cdd:cd17645 119 PKGVMIEHHN-------LVNLCEWHRP-----------YFGVTPADKSLVYASFS------FDAS-AWEIF-----PHLT 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 295 VFMAVPTIYSKL---MDYHDRHFGQPHVR-DFV-RAVCEE-------KIRLMVSGSAALPlpVLEKwkgaTGHTLLERYG 362
Cdd:cd17645 169 AGAALHVVPSERrldLDALNDYFNQEGITiSFLpTGAAEQfmqldnqSLRVLLTGGDKLK--KIER----KGYKLVNNYG 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 363 MTEIG-MALSNPLATARLPGSVGTPLPGVEVRIVSENPQKdgCPyiihaegnetetrvtpgfEETEGELLVRGPSVFRGY 441
Cdd:cd17645 243 PTENTvVATSFEIDKPYANIPIGKPIDNTRVYILDEALQL--QP------------------IGVAGELCIAGEGLARGY 302
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 442 WDKPEETKNAFTSDGW------FKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPD 514
Cdd:cd17645 303 LNRPELTAEKFIVHPFvpgermYRTGDLAKFlPDGNIEFLGRLD-QQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKED 381
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 515 VTWGQRVTAVVTLREghSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17645 382 ADGRKYLVAYVTAPE--EIPHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
55-574 |
1.37e-25 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 110.26 E-value: 1.37e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALADQhgshipAGSHTYRDLYSRSLSLAREicrLRacSDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRK 134
Cdd:cd17656 3 DAVAVVFE------NQKLTYRELNERSNQLARF---LR--EKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPE 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 135 HPQAELEYFIQDSRSSVVLAGQEHVELLSpvvrKLGVPLLPLPPTVYCGAAkdpgeEQVPERDWRDRGAMIIYTSGTTGR 214
Cdd:cd17656 72 YPEERRIYIMLDSGVRVVLTQRHLKSKLS----FNKSTILLEDPSISQEDT-----SNIDYINNSDDLLYIIYTSGTTGK 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 215 PKGVVITHHNVwatvTGLV-----HKWAWTKDDVILHVLPLHHV--HGVVNKLLcplwVGATCVMLPEFSAQLVWEKFLS 287
Cdd:cd17656 143 PKGVQLEHKNM----VNLLhfereKTNINFSDKVLQFATCSFDVcyQEIFSTLL----SGGTLYIIREETKRDVEQLFDL 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 288 SQSPRVNVfMAVPTIYSKLMdYHDRHFGQPhVRDFVRAVCEEKIRLMVSGsaalPLPVLEKWKGATGHTlleRYGMTE-- 365
Cdd:cd17656 215 VKRHNIEV-VFLPVAFLKFI-FSEREFINR-FPTCVKHIITAGEQLVITN----EFKEMLHEHNVHLHN---HYGPSEth 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 366 -IGMALSNPLATARLPGSVGTPLPGVEVRIVSENPQKDGCPYIihaegnetetrvtpgfeeteGELLVRGPSVFRGYWDK 444
Cdd:cd17656 285 vVTTYTINPEAEIPELPPIGKPISNTWIYILDQEQQLQPQGIV--------------------GELYISGASVARGYLNR 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 445 PEETKNAFTSDGW------FKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTW 517
Cdd:cd17656 345 QELTAEKFFPDPFdpnermYRTGDLARYlPDGNIEFLGRAD-HQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKG 423
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 743747175 518 GQRVTA-VVTLREghsLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17656 424 EKYLCAyFVMEQE---LNISQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
55-574 |
3.04e-25 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 109.03 E-value: 3.04e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 55 DRVALAdqHGSHipagSHTYRDLYSRSLSLAREICrlracSDGDLR-EERVSFLCSNDVSYVVAQWASWMSGGTAVPLYR 133
Cdd:cd17648 2 DRVAVV--YGDK----RLTYRELNERANRLAHYLL-----SVAEIRpDDLVGLVLDKSELMIIAILAVWKAGAAYVPIDP 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 134 KHPQAELEYFIQDSRSSVVLAGqehvellspvVRKLgvpllplpptvycgaakdpgeeqvperdwrdrgAMIIYTSGTTG 213
Cdd:cd17648 71 SYPDERIQFILEDTGARVVITN----------STDL---------------------------------AYAIYTSGTTG 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 214 RPKGVVITHHNVWATVTGLVHKW--AWTKDDVIL----HVLPLHhvhgvVNKLLCPLWVGATCVMLPEfSAQLVWEKFLS 287
Cdd:cd17648 108 KPKGVLVEHGSVVNLRTSLSERYfgRDNGDEAVLffsnYVFDFF-----VEQMTLALLNGQKLVVPPD-EMRFDPDRFYA 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 288 -SQSPRVNVFMAVPTIYSkLMDYHDRhfgqPHVRdfvravceekiRLMVSGSaALPLPVLEKWKGATGHTLLERYGMTEI 366
Cdd:cd17648 182 yINREKVTYLSGTPSVLQ-QYDLARL----PHLK-----------RVDAAGE-EFTAPVFEKLRSRFAGLIINAYGPTET 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 367 GM-ALSNP-LATARLPGSVGTPLPGVEVRIVSENPQkdgcpyiihaegnetetRVTPGfeeTEGELLVRGPSVFRGYWDK 444
Cdd:cd17648 245 TVtNHKRFfPGDQRFDKSLGRPVRNTKCYVLNDAMK-----------------RVPVG---AVGELYLGGDGVARGYLNR 304
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 445 PEETK-----NAFTSDG---------WFKTGDTAVFK-DGSYWIRGRTSVDiIKTGGYKVSALEVERLLLGHPSIADVAV 509
Cdd:cd17648 305 PELTAerflpNPFQTEQerargrnarLYKTGDLVRWLpSGELEYLGRNDFQ-VKIRGQRIEPGEVEAALASYPGVRECAV 383
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 510 IGVPDVTWGQ--RVTAVV---TLREGHsLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17648 384 VAKEDASQAQsrIQKYLVgyyLPEPGH-VPESDLLSFLRAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
194-570 |
4.37e-25 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 110.83 E-value: 4.37e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 194 PERDWRDRgAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVML 273
Cdd:PRK06814 788 CNRDPDDP-AVILFTSGSEGTPKGVVLSHRNLLANRAQVAARIDFSPEDKVFNALPVFHSFGLTGGLVLPLLSGVKVFLY 866
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 274 PefsaqlvwekflssqSPRVnvFMAVPT-IYSK-----------LMDYHDrhfgQPHVRDFVRavceekIRLMVSGSaal 341
Cdd:PRK06814 867 P---------------SPLH--YRIIPElIYDTnatilfgtdtfLNGYAR----YAHPYDFRS------LRYVFAGA--- 916
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 342 plpvlEKWKGAT--------GHTLLERYGMTEIG--MALSNPLATArlPGSVGTPLPGVEVRIVSenpqkdgcpyiihae 411
Cdd:PRK06814 917 -----EKVKEETrqtwmekfGIRILEGYGVTETApvIALNTPMHNK--AGTVGRLLPGIEYRLEP--------------- 974
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 412 gnetetrvTPGFEETeGELLVRGPSVFRGYW--DKP---EETKnaftsDGWFKTGD-TAVFKDGSYWIRGRTSvDIIKTG 485
Cdd:PRK06814 975 --------VPGIDEG-GRLFVRGPNVMLGYLraENPgvlEPPA-----DGWYDTGDiVTIDEEGFITIKGRAK-RFAKIA 1039
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 486 GYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRvtaVVTLREGHSLSHRELKEWARGVLAP-YAVPSELLLVEEIPRN 564
Cdd:PRK06814 1040 GEMISLAAVEELAAELWPDALHAAVSIPDARKGER---IILLTTASDATRAAFLAHAKAAGASeLMVPAEIITIDEIPLL 1116
|
....*.
gi 743747175 565 QMGKVN 570
Cdd:PRK06814 1117 GTGKID 1122
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
73-577 |
1.26e-24 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 107.57 E-value: 1.26e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLyrkhpqaeleyfiqdsrssVV 152
Cdd:cd05908 17 SYRHLREEALGYLGALQEL-----GIKPGQEVVFQITHNNKFLYLFWACLLGGMIAVPV-------------------SI 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHVELLSPVVRKLGVPLLPLPPTVYCGAAkdpgeeqvperdwrDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGL 232
Cdd:cd05908 73 GSNEEHKLKLNKVWNTLKNPYLITEEEVLCELA--------------DELAFIQFSSGSTGDPKGVMLTHENLVHNMFAI 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 233 VHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPefsaqlvwekflssqsprVNVFMAVPTIYSKLMDYHDR 312
Cdd:cd05908 139 LNSTEWKTKDRILSWMPLTHDMGLIAFHLAPLIAGMNQYLMP------------------TRLFIRRPILWLKKASEHKA 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 313 ------HFGQPHVRDFVRAVCEEK-----IRLMVSGSAALPLPVLEKWKGATGHTLLER------YGMTEIGMALSNPLA 375
Cdd:cd05908 201 tivsspNFGYKYFLKTLKPEKANDwdlssIRMILNGAEPIDYELCHEFLDHMSKYGLKRnailpvYGLAEASVGASLPKA 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 376 TARLPG----------------------------SVGTPLPGVEVRIVSEnpqkdgcpyiihaegnetETRVTPgfEETE 427
Cdd:cd05908 281 QSPFKTitlgrrhvthgepepevdkkdsecltfvEVGKPIDETDIRICDE------------------DNKILP--DGYI 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 428 GELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFKDGSYWIRGRTSvDIIKTGGYKV-------SALEVERLLLG 500
Cdd:cd05908 341 GHIQIRGKNVTPGYYNNPEATAKVFTDDGWLKTGDLGFIRNGRLVITGREK-DIIFVNGQNVyphdierIAEELEGVELG 419
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 501 HpsiadVAVIGVPDV-TWGQRVTAVVTLREghSLSHrelkewargvLAPYAVP-------------SELLLVEEIPRNQM 566
Cdd:cd05908 420 R-----VVACGVNNSnTRNEEIFCFIEHRK--SEDD----------FYPLGKKikkhlnkrggwqiNEVLPIRRIPKTTS 482
|
570
....*....|.
gi 743747175 567 GKVNKRDLVQQ 577
Cdd:cd05908 483 GKVKRYELAQR 493
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
49-498 |
3.10e-24 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 108.33 E-value: 3.10e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 49 RALAFGDRVAL---ADQHGShipAGSHTYRDLYSRSLSLAREICRLRACSDgdlreeRVSFLCSNDVSYVVAQWASWMSG 125
Cdd:PRK05691 18 RAAQTPDRLALrflADDPGE---GVVLSYRDLDLRARTIAAALQARASFGD------RAVLLFPSGPDYVAAFFGCLYAG 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 126 GTAVPLY-----RKHPQAELEYFIQDSRSSVVLAgqehVELLSPVVRKLGVPLLPLPPTVYCGAAKDPgeeqVPERDWR- 199
Cdd:PRK05691 89 VIAVPAYppesaRRHHQERLLSIIADAEPRLLLT----VADLRDSLLQMEELAAANAPELLCVDTLDP----ALAEAWQe 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 200 -----DRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWA--WTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVM 272
Cdd:PRK05691 161 palqpDDIAFLQYTSGSTALPKGVQVSHGNLVANEQLIRHGFGidLNPDDVIVSWLPLYHDMGLIGGLLQPIFSGVPCVL 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 273 LpefsaqlvwekflssqSPrvNVFMAVPTIY-SKLMDYHDRHFGQPhvrDFVRAVCEEKI-------------RLMVSGS 338
Cdd:PRK05691 241 M----------------SP--AYFLERPLRWlEAISEYGGTISGGP---DFAYRLCSERVsesalerldlsrwRVAYSGS 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 339 AALPLPVLEKWK------GATGHTLLERYGMTE-------------IGMALSNPLATAR---LPG------SVGTPLPGV 390
Cdd:PRK05691 300 EPIRQDSLERFAekfaacGFDPDSFFASYGLAEatlfvsggrrgqgIPALELDAEALARnraEPGtgsvlmSCGRSQPGH 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 391 EVRIVsENPQKDGCPyiihaegnetETRVtpgfeeteGELLVRGPSVFRGYWDKPEETKNAFTS-DG--WFKTGDTAVFK 467
Cdd:PRK05691 380 AVLIV-DPQSLEVLG----------DNRV--------GEIWASGPSIAHGYWRNPEASAKTFVEhDGrtWLRTGDLGFLR 440
|
490 500 510
....*....|....*....|....*....|.
gi 743747175 468 DGSYWIRGRTSvDIIKTGGYKVSALEVERLL 498
Cdd:PRK05691 441 DGELFVTGRLK-DMLIVRGHNLYPQDIEKTV 470
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
202-574 |
4.29e-24 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 104.73 E-value: 4.29e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 202 GAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVML----PEFS 277
Cdd:PRK08308 103 PSLLQYSSGTTGEPKLIRRSWTEIDREIEAYNEALNCEQDETPIVACPVTHSYGLICGVLAALTRGSKPVIItnknPKFA 182
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 278 AQLVwekflssQSPRVNVFMAVPTIYSKLMDyhdrhfgqphvrdFVRAvcEEKI-RLMVSGsAALPLPVLEKWKGATGHt 356
Cdd:PRK08308 183 LNIL-------RNTPQHILYAVPLMLHILGR-------------LLPG--TFQFhAVMTSG-TPLPEAWFYKLRERTTY- 238
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 357 LLERYGMTEIG-MALSNPLATarlPGSVGTPLPGVEVRIVS--ENPQKdgcpYIIHAEGNETETRvtpgfeetegELlvr 433
Cdd:PRK08308 239 MMQQYGCSEAGcVSICPDMKS---HLDLGNPLPHVSVSAGSdeNAPEE----IVVKMGDKEIFTK----------DL--- 298
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 434 gpsvfrGYWDKpeetknaftsdgwfktgdtavfkDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVP 513
Cdd:PRK08308 299 ------GYKSE-----------------------RGTLHFMGRMD-DVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGK 348
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 743747175 514 DVTWGQRVTAVVTLRegHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK08308 349 DPVAGERVKAKVISH--EEIDPVQLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
188-577 |
7.00e-23 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 103.25 E-value: 7.00e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 188 PGEEQVPERDwrDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVG 267
Cdd:PRK08043 355 PRLAQVKQQP--EDAALILFTSGSEGHPKGVVHSHKSLLANVEQIKTIADFTPNDRFMSALPLFHSFGLTVGLFTPLLTG 432
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 268 ATCVMLP-----EFSAQLVWEKflssqspRVNVFMAVPTIYSklmdyHDRHFGQPHvrDFVRavceekIRLMVSGSAALP 342
Cdd:PRK08043 433 AEVFLYPsplhyRIVPELVYDR-------NCTVLFGTSTFLG-----NYARFANPY--DFAR------LRYVVAGAEKLQ 492
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 343 LPVLEKWKGATGHTLLERYGMTEIG--MALSNPLATArlPGSVGTPLPGVEVRIVSenpqkdgcpyiihaegnetetrvT 420
Cdd:PRK08043 493 ESTKQLWQDKFGLRILEGYGVTECApvVSINVPMAAK--PGTVGRILPGMDARLLS-----------------------V 547
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 421 PGFEETeGELLVRGPSVFRGYW--DKP-------EETKNAFTSDGWFKTGDTAVFKDGSY-WIRGRTSvDIIKTGGYKVS 490
Cdd:PRK08043 548 PGIEQG-GRLQLKGPNIMNGYLrvEKPgvlevptAENARGEMERGWYDTGDIVRFDEQGFvQIQGRAK-RFAKIAGEMVS 625
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 491 ALEVERLLLGHPSIADVAVIGVPDVTWGQrvtAVVTLREGHSLSHRELKEWARGVLAP-YAVPSELLLVEEIPRNQMGK- 568
Cdd:PRK08043 626 LEMVEQLALGVSPDKQHATAIKSDASKGE---ALVLFTTDSELTREKLQQYAREHGVPeLAVPRDIRYLKQLPLLGSGKp 702
|
410
....*....|.
gi 743747175 569 --VNKRDLVQQ 577
Cdd:PRK08043 703 dfVTLKSMVDE 713
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
203-543 |
7.33e-23 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 102.67 E-value: 7.33e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVnkllcplwVGATCVmLPEFS----A 278
Cdd:PRK09274 177 AAILFTSGSTGTPKGVVYTHGMFEAQIEALREDYGIEPGEIDLPTFPLFALFGPA--------LGMTSV-IPDMDptrpA 247
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 QLVWEKFLSS-QSPRVNVFMAVPTIYSKLMDYhdrhfGQPHVRDFvravceEKIRLMVSGSAALPLPVLEKWKGATGHT- 356
Cdd:PRK09274 248 TVDPAKLFAAiERYGVTNLFGSPALLERLGRY-----GEANGIKL------PSLRRVISAGAPVPIAVIERFRAMLPPDa 316
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 357 -LLERYGMTE------IGM-----ALSNplATARLPGS-VGTPLPGVEVRIV--SENPqkdgcpyIIHAEGnetETRVTP 421
Cdd:PRK09274 317 eILTPYGATEalpissIESreilfATRA--ATDNGAGIcVGRPVDGVEVRIIaiSDAP-------IPEWDD---ALRLAT 384
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 422 GfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDG----WFKTGDTAVFKD-GSYWIRGRTSvDIIKTGGYKVSALEVER 496
Cdd:PRK09274 385 G---EIGEIVVAGPMVTRSYYNRPEATRLAKIPDGqgdvWHRMGDLGYLDAqGRLWFCGRKA-HRVETAGGTLYTIPCER 460
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|.
gi 743747175 497 LLLGHPSIADVAVIGVPdVTWGQRVTAVVTLREGHSLS----HRELKEWAR 543
Cdd:PRK09274 461 IFNTHPGVKRSALVGVG-VPGAQRPVLCVELEPGVACSksalYQELRALAA 510
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
204-572 |
7.48e-23 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 102.95 E-value: 7.48e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 204 MIIYTSGTTGRPKGVVITHHNV-WATVTGLVHKWAWTKDDVILHVLPLHHVHGVVnKLLCPLWVGATCVM---LPEF-SA 278
Cdd:cd05968 240 MIIYTSGTTGKPKGTVHVHAGFpLKAAQDMYFQFDLKPGDLLTWFTDLGWMMGPW-LIFGGLILGATMVLydgAPDHpKA 318
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 QLVWEkflSSQSPRVNVFMAVPTIYSKLMdyhdrhfgqPHVRDFVRAvcEEKIRLMVSGSAALPLPvLEKWKGATGHTLL 358
Cdd:cd05968 319 DRLWR---MVEDHEITHLGLSPTLIRALK---------PRGDAPVNA--HDLSSLRVLGSTGEPWN-PEPWNWLFETVGK 383
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 359 ERY------GMTEI-GMALSNPLATARLPGSVGTPLPGVEVRIVSEnpqkDGCPyiihaegnetetrvtpgFEETEGELL 431
Cdd:cd05968 384 GRNpiinysGGTEIsGGILGNVLIKPIKPSSFNGPVPGMKADVLDE----SGKP-----------------ARPEVGELV 442
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 432 VRGP--SVFRGYWDKPEETKNAFTS--DGWFKTGDTAVFKDGSYW-IRGRtSVDIIKTGGYKVSALEVERLLLGHPSIAD 506
Cdd:cd05968 443 LLAPwpGMTRGFWRDEDRYLETYWSrfDNVWVHGDFAYYDEEGYFyILGR-SDDTINVAGKRVGPAEIESVLNAHPAVLE 521
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 743747175 507 VAVIGVPDVTWGQRVTAVVTLREGHSLS---HRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKR 572
Cdd:cd05968 522 SAAIGVPHPVKGEAIVCFVVLKPGVTPTealAEELMERVADELGKPLSPERILFVKDLPKTRNAKVMRR 590
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
203-579 |
1.18e-22 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 102.26 E-value: 1.18e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVW 282
Cdd:PRK08279 202 AFYIYTSGTTGLPKAAVMSHMRWLKAMGGFGGLLRLTPDDVLYCCLPLYHNTGGTVAWSSVLAAGATLALRRKFSASRFW 281
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 283 EKFlssQSPRVNVFMAVPTIYSKLMDYhdrhfgQPHVRDfvRAvceEKIRLMVsGSAALPlPVLEKWKGATG-HTLLERY 361
Cdd:PRK08279 282 DDV---RRYRATAFQYIGELCRYLLNQ------PPKPTD--RD---HRLRLMI-GNGLRP-DIWDEFQQRFGiPRILEFY 345
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 362 GMTEIGMALSN----PLATARLPGSVGTPLPGVEVRIVSENPQKDgcpyiihAEGNetETRVTPGfeETeGELLV----R 433
Cdd:PRK08279 346 AASEGNVGFINvfnfDGTVGRVPLWLAHPYAIVKYDVDTGEPVRD-------ADGR--CIKVKPG--EV-GLLIGritdR 413
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 434 GPsvFRGYWDkPEETK-----NAFT-SDGWFKTGDTAVFKDGSYWirgrTSVDII------KtgGYKVSALEVERLLLGH 501
Cdd:PRK08279 414 GP--FDGYTD-PEASEkkilrDVFKkGDAWFNTGDLMRDDGFGHA----QFVDRLgdtfrwK--GENVATTEVENALSGF 484
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 502 PSIADVAVIGV--PDvTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQLY 579
Cdd:PRK08279 485 PGVEEAVVYGVevPG-TDGRAGMAAIVLADGAEFDLAALAAHLYERLPAYAVPLFVRLVPELETTGTFKYRKVDLRKEGF 563
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
207-574 |
5.63e-22 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 99.43 E-value: 5.63e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 207 YTSGTTGRPKGVVITHHNVW--ATVTGLVHKWAWTKDDVILHVLPLHHVHGvvnklLCPLWV----GATCVMLPEFSAQL 280
Cdd:cd05915 160 YTTGTTGLPKGVVYSHRALVlhSLAASLVDGTALSEKDVVLPVVPMFHVNA-----WCLPYAatlvGAKQVLPGPRLDPA 234
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 281 VWekFLSSQSPRVNVFMAVPTIYSKLMDyhdrhfgqphVRDFVRAVCEEKIRLMVSGSAalPLPVLEKWKGATGHTLLER 360
Cdd:cd05915 235 SL--VELFDGEGVTFTAGVPTVWLALAD----------YLESTGHRLKTLRRLVVGGSA--APRSLIARFERMGVEVRQG 300
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 361 YGMTEIgmalsNPLATARLPGSVGTPLPGVE-VRIVSenpqKDGCPYIIHA-EGNETETRVTPGFEETEGELLVRGPSVF 438
Cdd:cd05915 301 YGLTET-----SPVVVQNFVKSHLESLSEEEkLTLKA----KTGLPIPLVRlRVADEEGRPVPKDGKALGEVQLKGPWIT 371
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 439 RGYWDKPEETKNAFTSDGWFKTGDTAVFKDGSYWIRGRTSVDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWG 518
Cdd:cd05915 372 GGYYGNEEATRSALTPDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQ 451
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 519 QRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd05915 452 ERPLAVVVPRGEKPTPEELNEHLLKAGFAKWQLPDAYVFAEEIPRTSAGKFLKRAL 507
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
53-574 |
6.22e-22 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 101.01 E-value: 6.22e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 53 FGDRVALADQHGSHIPAGSH-TYRDLYSRSLSLAREicrLRACSDGDlrEERVSFLCSNDVSYVVAQWASWMSGGTAVPL 131
Cdd:PRK05691 2194 FAAQAARTPQAPALTFAGQTlSYAELDARANRLARA---LRERGVGP--QVRVGLALERSLEMVVGLLAILKAGGAYVPL 2268
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 132 YRKHPQAELEYFIQDSRSSVVLAGQEHVELLSPVvrKLGVPLLPLPPTVYCGAAKDPGEeqVPERDWRDRGAMIIYTSGT 211
Cdd:PRK05691 2269 DPEYPLERLHYMIEDSGIGLLLSDRALFEALGEL--PAGVARWCLEDDAAALAAYSDAP--LPFLSLPQHQAYLIYTSGS 2344
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 212 TGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLhHVHGVVNKLLCPLWVGATCVML------PEFSAQLVWEKf 285
Cdd:PRK05691 2345 TGKPKGVVVSHGEIAMHCQAVIERFGMRADDCELHFYSI-NFDAASERLLVPLLCGARVVLRaqgqwgAEEICQLIREQ- 2422
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 286 lssqspRVNVFMAVPTIYSKLMDYHDRHFGQPHVrdfvravceekiRLMVSGSAALPLPVLEKWKGATGHTLL-ERYGMT 364
Cdd:PRK05691 2423 ------QVSILGFTPSYGSQLAQWLAGQGEQLPV------------RMCITGGEALTGEHLQRIRQAFAPQLFfNAYGPT 2484
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 365 E-IGMALSNpLATARLPGSVGTPLPGvevRIVSENpqkdgCPYIIHAEgnetetrVTPGFEETEGELLVRGPSVFRGYWD 443
Cdd:PRK05691 2485 EtVVMPLAC-LAPEQLEEGAASVPIG---RVVGAR-----VAYILDAD-------LALVPQGATGELYVGGAGLAQGYHD 2548
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 444 KPEETKNAFTSDGW-------FKTGDTAVFK-DGSYWIRGRTSVDiIKTGGYKVSALEVERLLLGHPSIADVAVIGVpDV 515
Cdd:PRK05691 2549 RPGLTAERFVADPFaadggrlYRTGDLVRLRaDGLVEYVGRIDHQ-VKIRGFRIELGEIESRLLEHPAVREAVVLAL-DT 2626
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 743747175 516 TWGQR-----VTAVVTLREGHSLSHRE-LKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK05691 2627 PSGKQlagylVSAVAGQDDEAQAALREaLKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRAL 2691
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
199-476 |
7.33e-22 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 100.05 E-value: 7.33e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 199 RDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKW-----AWTKDDVILHVLPLHHV--HGVVNKLL---CPLWVGA 268
Cdd:PTZ00216 263 NDDLALIMYTSGTTGDPKGVMHTHGSLTAGILALEDRLndligPPEEDETYCSYLPLAHImeFGVTNIFLargALIGFGS 342
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 269 TCVMLPEFS---AQLV-WEKFLSSQSPRV------NVFMAVP-------TIYSK--------LMDYHDRHFGQPHVRDFV 323
Cdd:PTZ00216 343 PRTLTDTFArphGDLTeFRPVFLIGVPRIfdtikkAVEAKLPpvgslkrRVFDHayqsrlraLKEGKDTPYWNEKVFSAP 422
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 324 RAVCEEKIRLMVSGSAALPLPVLEkWKGATGHTLLERYGMTE---IGmalsnplATARL----PGSVGTPLPGVEVRIVS 396
Cdd:PTZ00216 423 RAVLGGRVRAMLSGGGPLSAATQE-FVNVVFGMVIQGWGLTEtvcCG-------GIQRTgdlePNAVGQLLKGVEMKLLD 494
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 397 ENPQKdgcpyiiHAEgnetetrvTPgfeETEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIRG 475
Cdd:PTZ00216 495 TEEYK-------HTD--------TP---EPRGEILLRGPFLFKGYYKQEELTREVLDEDGWFHTGDVGSIaANGTLRIIG 556
|
.
gi 743747175 476 R 476
Cdd:PTZ00216 557 R 557
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
203-511 |
8.75e-22 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 99.35 E-value: 8.75e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHN-VW---ATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGAtCVMLPEFSA 278
Cdd:cd05933 153 CTLIYTSGTTGMPKGVMLSHDNiTWtakAASQHMDLRPATVGQESVVSYLPLSHIAAQILDIWLPIKVGG-QVYFAQPDA 231
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 qLVWEKFLSSQSPRVNVFMAVPTIYSKLMDYHDRHFGQ--PHVRDFV----RAVCEEKIRLMvSGSAALPLP-------V 345
Cdd:cd05933 232 -LKGTLVKTLREVRPTAFMGVPRVWEKIQEKMKAVGAKsgTLKRKIAswakGVGLETNLKLM-GGESPSPLFyrlakklV 309
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 346 LEKWKGATG----HTLL----------------------ERYGMTEIGMA--LSNPLATaRLpGSVGTPLPGVEVRIvsE 397
Cdd:cd05933 310 FKKVRKALGldrcQKFFtgaapisretlefflslnipimELYGMSETSGPhtISNPQAY-RL-LSCGKALPGCKTKI--H 385
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 398 NPQKDGcpyiihaegnetetrvtpgfeetEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTA-VFKDGSYWIRGR 476
Cdd:cd05933 386 NPDADG-----------------------IGEICFWGRHVFMGYLNMEDKTEEAIDEDGWLHSGDLGkLDEDGFLYITGR 442
|
330 340 350
....*....|....*....|....*....|....*.
gi 743747175 477 TSVDIIKTGGYKVSALEVERLLLGH-PSIADVAVIG 511
Cdd:cd05933 443 IKELIITAGGENVPPVPIEDAVKKElPIISNAMLIG 478
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
203-577 |
1.07e-21 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 98.19 E-value: 1.07e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQLVW 282
Cdd:cd05940 84 ALYIYTSGTTGLPKAAIISHRRAWRGGAFFAGSGGALPSDVLYTCLPLYHSTALIVGWSACLASGATLVIRKKFSASNFW 163
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 283 EKFLSSQsprVNVFMAVPTIYSKLMDyhdrhfgQPHVRDfvravcEEKIRL-MVSGSAALPlPVLEKWKGATG-HTLLER 360
Cdd:cd05940 164 DDIRKYQ---ATIFQYIGELCRYLLN-------QPPKPT------ERKHKVrMIFGNGLRP-DIWEEFKERFGvPRIAEF 226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 361 YGMTEIGMALSNplaTARLPGSVG---------TPLPGVEVRIVSENPQKDGCPYIIhaegnetetRVTPGfeETeGELL 431
Cdd:cd05940 227 YAATEGNSGFIN---FFGKPGAIGrnpsllrkvAPLALVKYDLESGEPIRDAEGRCI---------KVPRG--EP-GLLI 291
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 432 VR--GPSVFRGYWDKPEETK----NAFTS-DGWFKTGDTAVFKDGSYW-IRGRTSvDIIKTGGYKVSALEVERLLLGHPS 503
Cdd:cd05940 292 SRinPLEPFDGYTDPAATEKkilrDVFKKgDAWFNTGDLMRLDGEGFWyFVDRLG-DTFRWKGENVSTTEVAAVLGAFPG 370
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 504 IADVAVIGV--PDvTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQ 577
Cdd:cd05940 371 VEEANVYGVqvPG-TDGRAGMAAIVLQPNEEFDLSALAAHLEKNLPGYARPLFLRLQPEMEITGTFKQQKVDLRNE 445
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
204-574 |
1.29e-21 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 97.64 E-value: 1.29e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 204 MIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVML--PEFSAQLV 281
Cdd:cd05974 89 LLYFTSGTTSKPKLVEHTHRSYPVGHLSTMYWIGLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATVFLFnyARFDAKRV 168
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 282 WEKFLSSqspRVNVFMAVPTIYsklmdyhdRHFGQPHVRDFvravcEEKIRLMVSGSAALPLPVLEKWKGATGHTLLERY 361
Cdd:cd05974 169 LAALVRY---GVTTLCAPPTVW--------RMLIQQDLASF-----DVKLREVVGAGEPLNPEVIEQVRRAWGLTIRDGY 232
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 362 GMTEIGMALSNPLATARLPGSVGTPLPGVEVRIVSenpqkdgcpyiihAEGNETetrvtpgfeeTEGELLV-----RGPS 436
Cdd:cd05974 233 GQTETTALVGNSPGQPVKAGSMGRPLPGYRVALLD-------------PDGAPA----------TEGEVALdlgdtRPVG 289
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 437 VFRGYWDKPEETKNAFtSDGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDV 515
Cdd:cd05974 290 LMKGYAGDPDKTAHAM-RGGYYRTGDIAMRdEDGYLTYVGRAD-DVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDP 367
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 743747175 516 TWGQRVTAVVTLREGHSLSH---RELKEWARGVLAPYAVPSELLLVeEIPRNQMGKVNKRDL 574
Cdd:cd05974 368 VRLSVPKAFIVLRAGYEPSPetaLEIFRFSRERLAPYKRIRRLEFA-ELPKTISGKIRRVEL 428
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
493-568 |
1.90e-21 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 88.37 E-value: 1.90e-21
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 493 EVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGK 568
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
403-578 |
2.85e-21 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 97.37 E-value: 2.85e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 403 GCP-------YIIHAEGNEtetrVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVF-KDGSYWIR 474
Cdd:PRK10946 356 GRPmspddevWVADADGNP----LPQG---EVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLVSIdPDGYITVV 428
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 475 GRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREghSLSHRELKEWAR--GVlAPYAVP 552
Cdd:PRK10946 429 GREK-DQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVKE--PLKAVQLRRFLReqGI-AEFKLP 504
|
170 180
....*....|....*....|....*.
gi 743747175 553 SELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PRK10946 505 DRVECVDSLPLTAVGKVDKKQLRQWL 530
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
70-574 |
3.38e-21 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 98.70 E-value: 3.38e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 70 GSHTYRDLYSRSLSLAReicRLRACSDGDlrEERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRS 149
Cdd:PRK05691 1155 GSLDYAELHAQANRLAH---YLRDKGVGP--DVCVAIAAERSPQLLVGLLAILKAGGAYVPLDPDYPAERLAYMLADSGV 1229
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 150 SVVLAgqeHVELLSPVVRKLGVpllplpptvyCGAAKDPGE-----EQVPERDWR-DRGAMIIYTSGTTGRPKGVVITHH 223
Cdd:PRK05691 1230 ELLLT---QSHLLERLPQAEGV----------SAIALDSLHldswpSQAPGLHLHgDNLAYVIYTSGSTGQPKGVGNTHA 1296
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 224 NVWATVTGLVHKWAWTKDDVILHVLPLhHVHGVVNKLLCPLWVGATCVML-------PEFSAQLVwekflssQSPRVNVF 296
Cdd:PRK05691 1297 ALAERLQWMQATYALDDSDVLMQKAPI-SFDVSVWECFWPLITGCRLVLAgpgehrdPQRIAELV-------QQYGVTTL 1368
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 297 MAVPTIYSKLMDyhdrhfgQPHVrdfvrAVCeEKIRLMVSGSAALPLP----VLEKWKGATGHTlleRYGMTEIGMALSN 372
Cdd:PRK05691 1369 HFVPPLLQLFID-------EPLA-----AAC-TSLRRLFSGGEALPAElrnrVLQRLPQVQLHN---RYGPTETAINVTH 1432
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 373 PLATA----RLPgsVGTPLPGVEVRIVSE--NPQKDGCPyiihaegnetetrvtpgfeeteGELLVRGPSVFRGYWDKPE 446
Cdd:PRK05691 1433 WQCQAedgeRSP--IGRPLGNVLCRVLDAelNLLPPGVA----------------------GELCIGGAGLARGYLGRPA 1488
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 447 ETKNAFTSDGW-------FKTGDTAVFK-DGSYWIRGRTSVDiIKTGGYKVSALEVERLLLGHPSIADVAVIgVPDVTWG 518
Cdd:PRK05691 1489 LTAERFVPDPLgedgarlYRTGDRARWNaDGALEYLGRLDQQ-VKLRGFRVEPEEIQARLLAQPGVAQAAVL-VREGAAG 1566
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 743747175 519 QRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK05691 1567 AQLVGYYTGEAGQEAEAERLKAALAAELPEYMVPAQLIRLDQMPLGPSGKLDRRAL 1622
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
58-574 |
6.27e-21 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 97.81 E-value: 6.27e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 58 ALADQHGSHipagshTYRDLYSRSLSLAREIcRLRACSDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQ 137
Cdd:PRK10252 476 ALADARYQF------SYREMREQVVALANLL-RERGVKPGD----SVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPD 544
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 138 AELEYFIQDSRSSVVLAGQEHVELLSPVvrklgvpllpLPPTVYCGAAKDPGEEQVPERDWR-DRGAMIIYTSGTTGRPK 216
Cdd:PRK10252 545 DRLKMMLEDARPSLLITTADQLPRFADV----------PDLTSLCYNAPLAPQGAAPLQLSQpHHTAYIIFTSGSTGRPK 614
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 217 GVVITHHnvwATVTGLV---HKWAWTKDDVILHVLPL-HHVHgvVNKLLCPLWVGATCVML-------PEFSAQLVWEKf 285
Cdd:PRK10252 615 GVMVGQT---AIVNRLLwmqNHYPLTADDVVLQKTPCsFDVS--VWEFFWPFIAGAKLVMAepeahrdPLAMQQFFAEY- 688
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 286 lssqspRVNVFMAVPTIYSKLMdyhdrhfGQPHVRDFVRAvCEEKIRLMVSGSaALPLPVLEKWKGATGHTLLERYGMTE 365
Cdd:PRK10252 689 ------GVTTTHFVPSMLAAFV-------ASLTPEGARQS-CASLRQVFCSGE-ALPADLCREWQQLTGAPLHNLYGPTE 753
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 366 IGMALSnplatarlpgsvGTPLPGVEVRIVSENPQKDGCP------YIIHAEGNEtetrVTPGfeeTEGELLVRGPSVFR 439
Cdd:PRK10252 754 AAVDVS------------WYPAFGEELAAVRGSSVPIGYPvwntglRILDARMRP----VPPG---VAGDLYLTGIQLAQ 814
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 440 GYWDKPEETKNAFTSDGW------FKTGDTAVFK-DGSYWIRGRtSVDIIKTGGYKVSALEVERLLLGHPSIADVA---- 508
Cdd:PRK10252 815 GYLGRPDLTASRFIADPFapgermYRTGDVARWLdDGAVEYLGR-SDDQLKIRGQRIELGEIDRAMQALPDVEQAVthac 893
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 743747175 509 VIGVPDVTWG--QRVTAVVTLREGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PRK10252 894 VINQAAATGGdaRQLVGYLVSQSGLPLDTSALQAQLRERLPPHMVPVVLLQLDQLPLSANGKLDRKAL 961
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
200-577 |
8.58e-21 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 95.58 E-value: 8.58e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 200 DRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQ 279
Cdd:cd05937 87 DDPAILIYTSGTTGLPKAAAISWRRTLVTSNLLSHDLNLKNGDRTYTCMPLYHGTAAFLGACNCLMSGGTLALSRKFSAS 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 280 LVWEKFLSSQSprvNVFMAVPTIYSKLMDYHDRHFGQPHvrdfvravceeKIRlMVSGSAALPlPVLEKWKgatghtllE 359
Cdd:cd05937 167 QFWKDVRDSGA---TIIQYVGELCRYLLSTPPSPYDRDH-----------KVR-VAWGNGLRP-DIWERFR--------E 222
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 360 RYGMTEIG---------MALSNPLATARLPGSVGtpLPGVEVRIVSENpqkDGCPYIIHAEGNETETRVTPGF-----EE 425
Cdd:cd05937 223 RFNVPEIGefyaategvFALTNHNVGDFGAGAIG--HHGLIRRWKFEN---QVVLVKMDPETDDPIRDPKTGFcvrapVG 297
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 426 TEGELLVRGP----SVFRGYWDKPEETKNAFTS------DGWFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSALEV 494
Cdd:cd05937 298 EPGEMLGRVPfknrEAFQGYLHNEDATESKLVRdvfrkgDIYFRTGDLLRQdADGRWYFLDRLG-DTFRWKSENVSTTEV 376
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 495 ERLLLGHPSIADVAVIGV--PDVTwGQRVTAVVTLREGHS----LSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGK 568
Cdd:cd05937 377 ADVLGAHPDIAEANVYGVkvPGHD-GRAGCAAITLEESSAvpteFTKSLLASLARKNLPSYAVPLFLRLTEEVATTDNHK 455
|
....*....
gi 743747175 569 VNKRDLVQQ 577
Cdd:cd05937 456 QQKGVLRDE 464
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
73-511 |
1.23e-20 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 96.06 E-value: 1.23e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREIcRLRACSDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PLN02861 79 TYKEVYDAAIRIGSAI-RSRGVNPGD----RCGIYGSNCPEWIIAMEACNSQGITYVPLYDTLGANAVEFIINHAEVSIA 153
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHVELLSPVVRKLgvpLLPLPPTVYCGAAKDPGEEQVPER-----DWRDRGAM-----------------IIYTSG 210
Cdd:PLN02861 154 FVQESKISSILSCLPKC---SSNLKTIVSFGDVSSEQKEEAEELgvscfSWEEFSLMgsldcelppkqktdictIMYTSG 230
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 211 TTGRPKGVVITHHNVWATVTGLVH-----KWAWTKDDVILHVLPLHHVHGVVNKLLCpLWVGATCVMlpefsaqlvWE-- 283
Cdd:PLN02861 231 TTGEPKGVILTNRAIIAEVLSTDHllkvtDRVATEEDSYFSYLPLAHVYDQVIETYC-ISKGASIGF---------WQgd 300
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 -KFL--SSQSPRVNVFMAVPTIY------------------SKLMDYH--------DRHFGQPHVRDFVRAVCEEKI--- 331
Cdd:PLN02861 301 iRYLmeDVQALKPTIFCGVPRVYdriytgimqkissggmlrKKLFDFAynyklgnlRKGLKQEEASPRLDRLVFDKIkeg 380
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 332 -----RLMVSGSAALPLPVLEKWKGATGHTLLERYGMTE-IGMALSNPLATARLPGSVGTPLPGVEVRIVSenpqkdgcp 405
Cdd:PLN02861 381 lggrvRLLLSGAAPLPRHVEEFLRVTSCSVLSQGYGLTEsCGGCFTSIANVFSMVGTVGVPMTTIEARLES--------- 451
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 406 yiIHAEGNETETRVtpgfeeTEGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTAVFK-DGSYWIRGRTSvDIIKT 484
Cdd:PLN02861 452 --VPEMGYDALSDV------PRGEICLRGNTLFSGYHKRQDLTEEVL-IDGWFHTGDIGEWQpNGAMKIIDRKK-NIFKL 521
|
490 500
....*....|....*....|....*...
gi 743747175 485 GGYKVSALEV-ERLLLGHPSIADVAVIG 511
Cdd:PLN02861 522 SQGEYVAVENlENTYSRCPLIASIWVYG 549
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
112-574 |
1.33e-20 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 96.77 E-value: 1.33e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 112 VSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAgQEHVellspvVRKLGVPLLPLPPTVYCGAAKDPGEE 191
Cdd:PRK12467 3156 VEMIVALLAVLKAGGAYVPLDPEYPRERLAYMIEDSGVKLLLT-QAHL------LEQLPAPAGDTALTLDRLDLNGYSEN 3228
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 192 QVPERDWRDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHhVHGVVNKLLCPLWVGATCV 271
Cdd:PRK12467 3229 NPSTRVMGENLAYVIYTSGSTGKPKGVGVRHGALANHLCWIAEAYELDANDRVLLFMSFS-FDGAQERFLWTLICGGCLV 3307
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 272 ML------PEFSAQLVwekflssQSPRVNVFMAVPTIYSKLMDYHDRHFGQPhvrdfvravceekIRLMVSGSAALPLPV 345
Cdd:PRK12467 3308 VRdndlwdPEELWQAI-------HAHRISIACFPPAYLQQFAEDAGGADCAS-------------LDIYVFGGEAVPPAA 3367
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 346 LEKWKGATGHT-LLERYGMTEIGMAL--------SNPLATArLPgsVGTPLPGVEVrivsenpqkdgcpYIIHAEGNETE 416
Cdd:PRK12467 3368 FEQVKRKLKPRgLTNGYGPTEAVVTVtlwkcggdAVCEAPY-AP--IGRPVAGRSI-------------YVLDGQLNPVP 3431
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 417 TRVTpgfeeteGELLVRGPSVFRGYWDKPEETKNAFTSDGW-------FKTGDTAVFK-DGSYWIRGRtsVD-IIKTGGY 487
Cdd:PRK12467 3432 VGVA-------GELYIGGVGLARGYHQRPSLTAERFVADPFsgsggrlYRTGDLARYRaDGVIEYLGR--IDhQVKIRGF 3502
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 488 KVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLShRELKEWARGVLAPYAVPSELLLVEEIPRNQMG 567
Cdd:PRK12467 3503 RIELGEIEARLLQHPSVREAVVLARDGAGGKQLVAYVVPADPQGDWR-ETLRDHLAASLPDYMVPAQLLVLAAMPLGPNG 3581
|
....*..
gi 743747175 568 KVNKRDL 574
Cdd:PRK12467 3582 KVDRKAL 3588
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
203-547 |
1.42e-20 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 95.58 E-value: 1.42e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDD--VILHVLPLHHVHGVVNKLLCPLWVGATCVM-----LPE 275
Cdd:cd05921 168 AKFLFTSGSTGLPKAVINTQRMLCANQAMLEQTYPFFGEEppVLVDWLPWNHTFGGNHNFNLVLYNGGTLYIddgkpMPG 247
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 276 FSAQLVweKFLSSQSPrvNVFMAVPTIYSKLMDYHDRHfgqphvrDFVRAVCEEKIRLMVSGSAALPLPVLEKWK----G 351
Cdd:cd05921 248 GFEETL--RNLREISP--TVYFNVPAGWEMLVAALEKD-------EALRRRFFKRLKLMFYAGAGLSQDVWDRLQalavA 316
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 352 ATGH--TLLERYGMTEIGMALSNPLATARLPGSVGTPLPGVEVRIVSenpqkdgcpyiihaEGNETETRvtpgfeetege 429
Cdd:cd05921 317 TVGEriPMMAGLGATETAPTATFTHWPTERSGLIGLPAPGTELKLVP--------------SGGKYEVR----------- 371
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 430 llVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFKDGS-----YWIRGRTSVDIIKTGGYKVS--ALEVERLLLGHP 502
Cdd:cd05921 372 --VKGPNVTPGYWRQPELTAQAFDEEGFYCLGDAAKLADPDdpakgLVFDGRVAEDFKLASGTWVSvgPLRARAVAACAP 449
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*
gi 743747175 503 SIADVAVIG----------VPDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLA 547
Cdd:cd05921 450 LVHDAVVAGedraevgalvFPDLLACRRLVGLQEASDAEVLRHAKVRAAFRDRLA 504
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
193-556 |
1.73e-20 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 94.55 E-value: 1.73e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 193 VPERDWR-DRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGvvnklLCPLW----VG 267
Cdd:PRK09029 127 AHAVAWQpQRLATMTLTSGSTGLPKAAVHTAQAHLASAEGVLSLMPFTAQDSWLLSLPLFHVSG-----QGIVWrwlyAG 201
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 268 ATCVmLPEFsaqlvwEKFLSSQSprvNVFMA--VPTIYSKLMDYHDRHFGQPHVrdfvravceekirLMvsGSAALPLPV 345
Cdd:PRK09029 202 ATLV-VRDK------QPLEQALA---GCTHAslVPTQLWRLLDNRSEPLSLKAV-------------LL--GGAAIPVEL 256
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 346 LEK--------WKGatghtllerYGMTEigMALSnplATAR----LPGsVGTPLPGVEVRIVsenpqkdgcpyiihaegn 413
Cdd:PRK09029 257 TEQaeqqgircWCG---------YGLTE--MAST---VCAKradgLAG-VGSPLPGREVKLV------------------ 303
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 414 etetrvtpgfeetEGELLVRGPSVFRGYWDKPEETknAFT-SDGWFKTGDTAVFKDGSYWIRGRTSVDIIkTGGYKVSAL 492
Cdd:PRK09029 304 -------------DGEIWLRGASLALGYWRQGQLV--PLVnDEGWFATRDRGEWQNGELTILGRLDNLFF-SGGEGIQPE 367
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 743747175 493 EVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVtlrEGHSLSHRE-LKEWARGVLA----P---YAVPSELL 556
Cdd:PRK09029 368 EIERVINQHPLVQQVFVVPVADAEFGQRPVAVV---ESDSEAAVVnLAEWLQDKLArfqqPvayYLLPPELK 436
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
203-579 |
1.75e-20 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 95.33 E-value: 1.75e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDD--VILHVLPLHHVHGVVNKLLCPLWVGATCVM-----LPE 275
Cdd:PRK08180 212 AKFLFTSGSTGLPKAVINTHRMLCANQQMLAQTFPFLAEEppVLVDWLPWNHTFGGNHNLGIVLYNGGTLYIddgkpTPG 291
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 276 FSAQLVweKFLSSQSPrvNVFMAVPTIYSKLMDYHDRhfgqphvrDfvRAVCE---EKIRLMVSGSAALPLPVLEKWKGA 352
Cdd:PRK08180 292 GFDETL--RNLREISP--TVYFNVPKGWEMLVPALER--------D--AALRRrffSRLKLLFYAGAALSQDVWDRLDRV 357
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 353 TGHTLLER------YGMTEIGMALSNPLATARLPGSVGTPLPGVEVRIVsenpqkdgcPYiihaeGNETETRvtpgfeet 426
Cdd:PRK08180 358 AEATCGERirmmtgLGMTETAPSATFTTGPLSRAGNIGLPAPGCEVKLV---------PV-----GGKLEVR-------- 415
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 427 egellVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFKDGSYWIR-----GRTSVDIIKTGGYKVS--ALEVERLLL 499
Cdd:PRK08180 416 -----VKGPNVTPGYWRAPELTAEAFDEEGYYRSGDAVRFVDPADPERglmfdGRIAEDFKLSSGTWVSvgPLRARAVSA 490
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 500 GHPSIADVAVIG----------VPDVTWGQRVTAvvtLREGHS----LSHRELKEWARGVLAPY--------AVPSELLL 557
Cdd:PRK08180 491 GAPLVQDVVITGhdrdeigllvFPNLDACRRLAG---LLADASlaevLAHPAVRAAFRERLARLnaqatgssTRVARALL 567
|
410 420 430
....*....|....*....|....*....|....*
gi 743747175 558 VEEIPRNQMGKV------NKR-------DLVQQLY 579
Cdd:PRK08180 568 LDEPPSLDAGEItdkgyiNQRavlarraALVEALY 602
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
205-574 |
2.32e-20 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 95.35 E-value: 2.32e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITH--HNVWaTVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVML---PEF-SA 278
Cdd:PLN02654 280 LLYTSGSTGKPKGVLHTTggYMVY-TATTFKYAFDYKPTDVYWCTADCGWITGHSYVTYGPMLNGATVLVFegaPNYpDS 358
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 QLVWE---KFlssqspRVNVFMAVPTIYSKLMDYHDRHFGQpHVRDFVRavceekirlmVSGSAALPL-PVLEKW----K 350
Cdd:PLN02654 359 GRCWDivdKY------KVTIFYTAPTLVRSLMRDGDEYVTR-HSRKSLR----------VLGSVGEPInPSAWRWffnvV 421
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 351 GATGHTLLERYGMTEIGMALSNPLATA--RLPGSVGTPLPGVEVRIVSENpqkdgcpyiihaeGNETETrvtpgfeETEG 428
Cdd:PLN02654 422 GDSRCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEK-------------GKEIEG-------ECSG 481
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 429 ELLVRG--PSVFRGYWDKPE--ETKNAFTSDGWFKTGD-TAVFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPS 503
Cdd:PLN02654 482 YLCVKKswPGAFRTLYGDHEryETTYFKPFAGYYFSGDgCSRDKDGYYWLTGRVD-DVINVSGHRIGTAEVESALVSHPQ 560
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 743747175 504 IADVAVIGVPDVTWGQRVTAVVTLREGHSLS---HRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:PLN02654 561 CAEAAVVGIEHEVKGQGIYAFVTLVEGVPYSeelRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 634
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
73-550 |
5.01e-20 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 94.11 E-value: 5.01e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREicrLRACsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PLN02430 78 TYKEVYEEVLQIGSA---LRAS--GAEPGSRVGIYGSNCPQWIVAMEACAAHSLICVPLYDTLGPGAVDYIVDHAEIDFV 152
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHV-ELLSPVVRKLGVPLLPLPPTVYCGAAKDPGEE-QVPERDWRDRGAM------------------IIYTSGTT 212
Cdd:PLN02430 153 FVQDKKIkELLEPDCKSAKRLKAIVSFTSVTEEESDKASQiGVKTYSWIDFLHMgkenpsetnppkpldictIMYTSGTS 232
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 213 GRPKGVVITHHNVWATVTGL-----VHKWAWTKDDVILHVLPLHHV-------------------HGVVNKLLCPLWVga 268
Cdd:PLN02430 233 GDPKGVVLTHEAVATFVRGVdlfmeQFEDKMTHDDVYLSFLPLAHIldrmieeyffrkgasvgyyHGDLNALRDDLME-- 310
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 269 tcvMLPEFSAQL--VWEKF-------LSSQSP-RVNVFMAVPTIYSKLMDYHDRHFGQPHVRDF-----VRAVCEEKIRL 333
Cdd:PLN02430 311 ---LKPTLLAGVprVFERIhegiqkaLQELNPrRRLIFNALYKYKLAWMNRGYSHKKASPMADFlafrkVKAKLGGRLRL 387
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 334 MVSGSAALPLPVLEKWKGATGHTLLERYGMTEI--GMALSNPLATARLpGSVGTPLPGVEVRIvSENPqkdgcpyiihae 411
Cdd:PLN02430 388 LISGGAPLSTEIEEFLRVTSCAFVVQGYGLTETlgPTTLGFPDEMCML-GTVGAPAVYNELRL-EEVP------------ 453
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 412 gnetETRVTPGFEETEGELLVRGPSVFRGYWDKPEETKNAFtSDGWFKTGDTA-VFKDGSYWIRGRTSvDIIKTGGYKVS 490
Cdd:PLN02430 454 ----EMGYDPLGEPPRGEICVRGKCLFSGYYKNPELTEEVM-KDGWFHTGDIGeILPNGVLKIIDRKK-NLIKLSQGEYV 527
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 743747175 491 ALE-VERLLLGHPSIADVAVIGvpdVTWGQRVTAVVTLREghslshRELKEWAR--GVLAPYA 550
Cdd:PLN02430 528 ALEyLENVYGQNPIVEDIWVYG---DSFKSMLVAVVVPNE------ENTNKWAKdnGFTGSFE 581
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
203-468 |
1.61e-19 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 92.42 E-value: 1.61e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHH---NVWATVTGLVHKWAWTKDDVILHVLPLHHVHGvVNKLLCP-LWVGATCVM-----L 273
Cdd:PRK12582 223 AKYLFTSGSTGMPKAVINTQRmmcANIAMQEQLRPREPDPPPPVSLDWMPWNHTMG-GNANFNGlLWGGGTLYIddgkpL 301
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 274 PEFSAQLVweKFLSSQSPRvnVFMAVPTIYSKLMDYHDRHfgqphvrDFVRAVCEEKIRLMVSGSAALPLPVLEKWKG-- 351
Cdd:PRK12582 302 PGMFEETI--RNLREISPT--VYGNVPAGYAMLAEAMEKD-------DALRRSFFKNLRLMAYGGATLSDDLYERMQAla 370
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 352 --ATGH--TLLERYGMTEI-GMALSNPLATARlPGSVGTPLPGVEVRIVsenpqkdgcpyiihAEGNETETRvtpgfeet 426
Cdd:PRK12582 371 vrTTGHriPFYTGYGATETaPTTTGTHWDTER-VGLIGLPLPGVELKLA--------------PVGDKYEVR-------- 427
|
250 260 270 280
....*....|....*....|....*....|....*....|..
gi 743747175 427 egellVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTAVFKD 468
Cdd:PRK12582 428 -----VKGPNVTPGYHKDPELTAAAFDEEGFYRLGDAARFVD 464
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
203-574 |
2.80e-19 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 90.61 E-value: 2.80e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCpLWVGATCVMLPEfSAQLVW 282
Cdd:cd17654 121 AYVIHTSGTTGTPKIVAVPHKCILPNIQHFRSLFNITSEDILFLTSPLTFDPSVVEIFLS-LSSGATLLIVPT-SVKVLP 198
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 283 EKFLSS--QSPRVNVFMAVPTIYsklmdyhdRHFGQPHVRDFVRAVcEEKIRLMVSGSAALP-LPVLEKWKGATGHT-LL 358
Cdd:cd17654 199 SKLADIlfKRHRITVLQATPTLF--------RRFGSQSIKSTVLSA-TSSLRVLALGGEPFPsLVILSSWRGKGNRTrIF 269
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 359 ERYGMTEIGM-ALSNPLATARLPGSVGTPLPGVEVRIVSENPQkdgcpyiihaegnetetrvtpgfeETEGELLVRGPSV 437
Cdd:cd17654 270 NIYGITEVSCwALAYKVPEEDSPVQLGSPLLGTVIEVRDQNGS------------------------EGTGQVFLGGLNR 325
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 438 fRGYWDKPEETKNAFtsdgWFKTGDTAVFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAvigvpdVTW 517
Cdd:cd17654 326 -VCILDDEVTVPKGT----MRATGDFVTVKDGELFFLGRKD-SQIKRRGKRINLDLIQQVIESCLGVESCA------VTL 393
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*....
gi 743747175 518 --GQRVTAVVTLREGHSLSHRELKewaRGVLAPYAVPSELLLVEEIPRNQMGKVNKRDL 574
Cdd:cd17654 394 sdQQRLIAFIVGESSSSRIHKELQ---LTLLSSHAIPDTFVQIDKLPLTSHGKVDKSEL 449
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
73-476 |
2.96e-19 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 91.62 E-value: 2.96e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 73 TYRDLYSRSLSLAREicrLRACSDGDlrEERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVV 152
Cdd:PLN02614 81 TYQEVYDIVIKLGNS---LRSVGVKD--EAKCGIYGANSPEWIISMEACNAHGLYCVPLYDTLGAGAVEFIISHSEVSIV 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 153 LAGQEHV-----------ELLSPVV-------------RKLGVPLLPLPPTVYCGAAKdpgEEQVPERDWRDRgAMIIYT 208
Cdd:PLN02614 156 FVEEKKIselfktcpnstEYMKTVVsfggvsreqkeeaETFGLVIYAWDEFLKLGEGK---QYDLPIKKKSDI-CTIMYT 231
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 209 SGTTGRPKGVVITHHNVWATVTGLVH-----KWAWTKDDVILHVLPLHHVHGVVNKLlCPLWVGATcVMLPEFSAQLVWE 283
Cdd:PLN02614 232 SGTTGDPKGVMISNESIVTLIAGVIRllksaNAALTVKDVYLSYLPLAHIFDRVIEE-CFIQHGAA-IGFWRGDVKLLIE 309
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 KfLSSQSPrvNVFMAVPTI----YS----KLMD-------YHDRHF---------GQPHVR----------DFVRAVCEE 329
Cdd:PLN02614 310 D-LGELKP--TIFCAVPRVldrvYSglqkKLSDggflkkfVFDSAFsykfgnmkkGQSHVEasplcdklvfNKVKQGLGG 386
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 330 KIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTE--IGMALSNPLATARLpGSVGTPLPGVEVRIVSenpqkdgCPyi 407
Cdd:PLN02614 387 NVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTEscAGTFVSLPDELDML-GTVGPPVPNVDIRLES-------VP-- 456
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 408 ihaEGNETETRVTPgfeetEGELLVRGPSVFRGYWDKPEETKNAFTsDGWFKTGDTAVFK-DGSYWIRGR 476
Cdd:PLN02614 457 ---EMEYDALASTP-----RGEICIRGKTLFSGYYKREDLTKEVLI-DGWLHTGDVGEWQpNGSMKIIDR 517
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
74-568 |
3.59e-19 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 91.22 E-value: 3.59e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 74 YRDLYSRSLSLAREICRLracsdGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLY-------RKHPQAELEYFIQD 146
Cdd:PRK09192 52 YQTLRARAEAGARRLLAL-----GLKPGDRVALIAETDGDFVEAFFACQYAGLVPVPLPlpmgfggRESYIAQLRGMLAS 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 147 SRSSVVLAGQEHVELLSPVVRKLGvpllplppTVYCGAAKDPGEEQVPERDWR----DRGAMIIYTSGTTGRPKGVVITH 222
Cdd:PRK09192 127 AQPAAIITPDELLPWVNEATHGNP--------LLHVLSHAWFKALPEADVALPrptpDDIAYLQYSSGSTRFPRGVIITH 198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 223 HNVWATVTGLVHKWAWTKD-DVILHVLPLHHVHGVVNKLLCPLWVGATCVMLP--EFSAQ-LVWEKFLS------SQSP- 291
Cdd:PRK09192 199 RALMANLRAISHDGLKVRPgDRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLPtrDFARRpLQWLDLISrnrgtiSYSPp 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 292 --------RVNVfmavptiySKLMDY---HDRHFG------QPHV-RDFVRAVCEEKIR---LMVS---GSAALPLPVLE 347
Cdd:PRK09192 279 fgyelcarRVNS--------KDLAELdlsCWRVAGigadmiRPDVlHQFAEAFAPAGFDdkaFMPSyglAEATLAVSFSP 350
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 348 KWKGATGHTLlERYGMTEIGMALSNPLATARLPGSV--GTPLPGVEVRIVSENPQkdgcpyiihaegnetetrVTPgfEE 425
Cdd:PRK09192 351 LGSGIVVEEV-DRDRLEYQGKAVAPGAETRRVRTFVncGKALPGHEIEIRNEAGM------------------PLP--ER 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 426 TEGELLVRGPSVFRGYWDKpEETKNAFTSDGWFKTGDTAVFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSI- 504
Cdd:PRK09192 410 VVGHICVRGPSLMSGYFRD-EESQDVLAADGWLDTGDLGYLLDGYLYITGRAK-DLIIINGRNIWPQDIEWIAEQEPELr 487
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 743747175 505 -ADVAVIGVPDVTwGQRVTAVVTLREGHSLSHRELKEWARGVL-APYAVPSELLLV--EEIPRNQMGK 568
Cdd:PRK09192 488 sGDAAAFSIAQEN-GEKIVLLVQCRISDEERRGQLIHALAALVrSEFGVEAAVELVppHSLPRTSSGK 554
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
205-576 |
7.13e-18 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 86.87 E-value: 7.13e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITHHNvwatvtgLVHKWAWTKDD--------------------VI-----------LHVLPlhhv 253
Cdd:PRK04813 148 IIFTSGTTGKPKGVQISHDN-------LVSFTNWMLEDfalpegpqflnqapysfdlsVMdlyptlasggtLVALP---- 216
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 254 HGVVN--KLL------CPL--WVgAT------CVMLPEFSAQlvwekflssQSPRVNVFMavptiysklmdyhdrhfgqp 317
Cdd:PRK04813 217 KDMTAnfKQLfetlpqLPInvWV-STpsfadmCLLDPSFNEE---------HLPNLTHFL-------------------- 266
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 318 hvrdFvravCEEkirlmvsgsaALPLPVLEKwkgatghtLLER---------YGMTEIGMALSNPLATA-------RLPg 381
Cdd:PRK04813 267 ----F----CGE----------ELPHKTAKK--------LLERfpsatiyntYGPTEATVAVTSIEITDemldqykRLP- 319
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 382 sVGTPLPGVEVRIVSENPQKdgcpyiiHAEGNEtetrvtpgfeeteGELLVRGPSVFRGYWDKPEETKNAF-TSDGW--F 458
Cdd:PRK04813 320 -IGYAKPDSPLLIIDEEGTK-------LPDGEQ-------------GEIVISGPSVSKGYLNNPEKTAEAFfTFDGQpaY 378
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 459 KTGDTAVFKDGSYWIRGRtsVDI-IKTGGYKVSALEVERLLLGHPSIAdVAVIgVPdVTWGQRVT---AVVTLREGH--- 531
Cdd:PRK04813 379 HTGDAGYLEDGLLFYQGR--IDFqIKLNGYRIELEEIEQNLRQSSYVE-SAVV-VP-YNKDHKVQyliAYVVPKEEDfer 453
|
410 420 430 440
....*....|....*....|....*....|....*....|....*..
gi 743747175 532 --SLSHrELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQ 576
Cdd:PRK04813 454 efELTK-AIKKELKERLMEYMIPRKFIYRDSLPLTPNGKIDRKALIE 499
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
203-560 |
3.30e-17 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 85.17 E-value: 3.30e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTG---LVHKWAwtKDDVILHVLPLHHVhgvvnkllcpLWVGATCVMLPEFSA- 278
Cdd:PLN02387 253 AVIMYTSGSTGLPKGVMMTHGNIVATVAGvmtVVPKLG--KNDVYLAYLPLAHI----------LELAAESVMAAVGAAi 320
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 279 ----QLVwekfLSSQSPRV-------------NVFMAVPTIYSKLMD--------------------YHDR-------HF 314
Cdd:PLN02387 321 gygsPLT----LTDTSNKIkkgtkgdasalkpTLMTAVPAILDRVRDgvrkkvdakgglakklfdiaYKRRlaaiegsWF 396
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 315 G----QPHVRDF-----VRAVCEEKIRLMVSGSAALplpvlekwKGAT--------GHTLLERYGMTEI--GMALSNPLA 375
Cdd:PLN02387 397 GawglEKLLWDAlvfkkIRAVLGGRIRFMLSGGAPL--------SGDTqrfiniclGAPIGQGYGLTETcaGATFSEWDD 468
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 376 TArlPGSVGTPLPGVEVRIVSenpqkdgcpyiiHAEGN-ETETRVTPgfeetEGELLVRGPSVFRGYWDKPEETKNAFTS 454
Cdd:PLN02387 469 TS--VGRVGPPLPCCYVKLVS------------WEEGGyLISDKPMP-----RGEIVIGGPSVTLGYFKNQEKTDEVYKV 529
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 455 DG----WFKTGDTAVFK-DGSYWIRGRTSvDIIKT--GGYkVSALEVERLLLGHPSIADVAVIGVPDVTWGqrVTAVVTl 527
Cdd:PLN02387 530 DErgmrWFYTGDIGQFHpDGCLEIIDRKK-DIVKLqhGEY-VSLGKVEAALSVSPYVDNIMVHADPFHSYC--VALVVP- 604
|
410 420 430
....*....|....*....|....*....|...
gi 743747175 528 reghslSHRELKEWARGVLAPYAVPSELLLVEE 560
Cdd:PLN02387 605 ------SQQALEKWAKKAGIDYSNFAELCEKEE 631
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
200-549 |
4.02e-17 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 84.05 E-value: 4.02e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 200 DRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVnkllcplwVGATCV---MLPEF 276
Cdd:cd05910 85 DEPAAILFTSGSTGTPKGVVYRHGTFAAQIDALRQLYGIRPGEVDLATFPLFALFGPA--------LGLTSVipdMDPTR 156
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 277 SAQLVWEKFLSS-QSPRVNVFMAVPTIYSKLMDYHDRHFGQ-PHVRDFVRAVCEEKIRLMVSGSAALPlpvlekwkgaTG 354
Cdd:cd05910 157 PARADPQKLVGAiRQYGVSIVFGSPALLERVARYCAQHGITlPSLRRVLSAGAPVPIALAARLRKMLS----------DE 226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 355 HTLLERYGMTE---IGMALSNPLATARLPGS-------VGTPLPGVEVRIVSENPQKdgcpyiIHAEGNETEtrVTPGfe 424
Cdd:cd05910 227 AEILTPYGATEalpVSSIGSRELLATTTAATsggagtcVGRPIPGVRVRIIEIDDEP------IAEWDDTLE--LPRG-- 296
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 425 eTEGELLVRGPSVFRGYWDKPEETKNAFTSDG----WFKTGDTAVFKD-GSYWIRGRTSVDIIKTGGyKVSALEVERLLL 499
Cdd:cd05910 297 -EIGEITVTGPTVTPTYVNRPVATALAKIDDNsegfWHRMGDLGYLDDeGRLWFCGRKAHRVITTGG-TLYTEPVERVFN 374
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|
gi 743747175 500 GHPSIADVAVIGVpDVTWGQRVTAVVTLREGHSLSHRELKEWARGVLAPY 549
Cdd:cd05910 375 THPGVRRSALVGV-GKPGCQLPVLCVEPLPGTITPRARLEQELRALAKDY 423
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
72-496 |
6.16e-17 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 83.89 E-value: 6.16e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 72 HTYRDLYSRSLSLAReicRLRAcsDGDLREERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSR--- 148
Cdd:PRK07768 30 HTWGEVHERARRIAG---GLAA--AGVGPGDAVAVLAGAPVEIAPTAQGLWMRGASLTMLHQPTPRTDLAVWAEDTLrvi 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 149 ----SSVVLAGqEHVELLSPVVRKLGVPLLPLPPTVycgAAKDPGEEQVPERDWrdrgAMIIYTSGTTGRPKGVVITHHN 224
Cdd:PRK07768 105 gmigAKAVVVG-EPFLAAAPVLEEKGIRVLTVADLL---AADPIDPVETGEDDL----ALMQLTSGSTGSPKAVQITHGN 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 225 VWATVTGLVHKWAWTKD-DVILHVLPLHHVHGVVNKLLCPLWVGATCVMLP--EFSAQ-LVWEKFLSSQspRVNVFMAVP 300
Cdd:PRK07768 177 LYANAEAMFVAAEFDVEtDVMVSWLPLFHDMGMVGFLTVPMYFGAELVKVTpmDFLRDpLLWAELISKY--RGTMTAAPN 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 301 TIYSKLmdyhdrhfgqphVRDFVRAVCEE-----KIRLMVSGSAALPLPVLEKWKGATG------HTLLERYGMTEIGMA 369
Cdd:PRK07768 255 FAYALL------------ARRLRRQAKPGafdlsSLRFALNGAEPIDPADVEDLLDAGArfglrpEAILPAYGMAEATLA 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 370 LS---------------------------NPLATARLPgSVGTPLPGVEVRIVSEnpqkDGcpyiihaegnetetRVTPg 422
Cdd:PRK07768 323 VSfspcgaglvvdevdadllaalrravpaTKGNTRRLA-TLGPPLPGLEVRVVDE----DG--------------QVLP- 382
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 743747175 423 fEETEGELLVRGPSVFRGYW--DKPEETKNAftsDGWFKTGDTAVFKD-GSYWIRGRTSvDIIKTGGYKVSALEVER 496
Cdd:PRK07768 383 -PRGVGVIELRGESVTPGYLtmDGFIPAQDA---DGWLDTGDLGYLTEeGEVVVCGRVK-DVIIMAGRNIYPTDIER 454
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
203-513 |
6.14e-16 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 81.02 E-value: 6.14e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 203 AMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVmlpeFSAQLVW 282
Cdd:PRK06334 186 AVILFTSGTEKLPKGVPLTHANLLANQRACLKFFSPKEDDVMMSFLPPFHAYGFNSCTLFPLLSGVPVV----FAYNPLY 261
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 283 EKFLSS--QSPRVNVFMAVPTIYSKLMDYHDRhfgqphvrdfvRAVCEEKIRLMVSGSAALPLPVLEKWKGATGH-TLLE 359
Cdd:PRK06334 262 PKKIVEmiDEAKVTFLGSTPVFFDYILKTAKK-----------QESCLPSLRFVVIGGDAFKDSLYQEALKTFPHiQLRQ 330
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 360 RYGMTEIGMALS-NPLATARLPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaegnETETRVTPGfeeTEGELLVRGPSVF 438
Cdd:PRK06334 331 GYGTTECSPVITiNTVNSPKHESCVGMPIRGMDVLIVSE----------------ETKVPVSSG---ETGLVLTRGTSLF 391
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 439 RGYWDkpEETKNAFTS---DGWFKTGDTA-VFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLGH------PSIADVA 508
Cdd:PRK06334 392 SGYLG--EDFGQGFVElggETWYVTGDLGyVDRHGELFLKGRLS-RFVKIGAEMVSLEALESILMEGfgqnaaDHAGPLV 468
|
....*
gi 743747175 509 VIGVP 513
Cdd:PRK06334 469 VCGLP 473
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
68-476 |
6.20e-16 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 81.14 E-value: 6.20e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 68 PAG---SHTYRDLYSRSLSLAREicrLRAC-SDGDlreeRVSFLCSNDVSYVVAQWASWMSGGTAVPLyrKHPQaeleYF 143
Cdd:PRK05850 29 PAGvaeTLTWSQLYRRTLNVAEE---LRRHgSTGD----RAVILAPQGLEYIVAFLGALQAGLIAVPL--SVPQ----GG 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 144 IQDSRSSVVLAGQEHVELL--SPVVRKLGvpllpLPPTVYCGAAK-----------DPGEEQVPERDWRDRGAMIIYTSG 210
Cdd:PRK05850 96 AHDERVSAVLRDTSPSVVLttSAVVDDVT-----EYVAPQPGQSAppvievdlldlDSPRGSDARPRDLPSTAYLQYTSG 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 211 TTGRPKGVVITHHNVWATVTGLV-------HKWAwTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSaqlvwe 283
Cdd:PRK05850 171 STRTPAGVMVSHRNVIANFEQLMsdyfgdtGGVP-PPDTTVVSWLPFYHDMGLVLGVCAPILGGCPAVLTSPVA------ 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 kflssqsprvnvFMAVPTIYSKLMDYHDRHF-GQPhvrDF-----VRAVCEE--------KIRLMVSGSaalplpvlEKW 349
Cdd:PRK05850 244 ------------FLQRPARWMQLLASNPHAFsAAP---NFafelaVRKTSDDdmagldlgGVLGIISGS--------ERV 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 350 KGATGHTLLER---YGMTEIGMALSNPLATARLpgSVGTPLPGVEVRIVSENPQK--DGcpyiiHAEGNETET------- 417
Cdd:PRK05850 301 HPATLKRFADRfapFNLRETAIRPSYGLAEATV--YVATREPGQPPESVRFDYEKlsAG-----HAKRCETGGgtplvsy 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 418 --------RVTPGFEETE------GELLVRGPSVFRGYWDKPEETKNAF----------TSDG-WFKTGDTAVFKDGSYW 472
Cdd:PRK05850 374 gsprsptvRIVDPDTCIEcpagtvGEIWVHGDNVAAGYWQKPEETERTFgatlvdpspgTPEGpWLRTGDLGFISEGELF 453
|
....
gi 743747175 473 IRGR 476
Cdd:PRK05850 454 IVGR 457
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
35-560 |
1.43e-15 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 79.81 E-value: 1.43e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 35 GPAAR--GNRSAPVFTRALA-FGDRVALA--DQHGSHIPAGSHT------------YRDLYSRSLSLAREICRLRACSDG 97
Cdd:cd17632 14 TEAIRrpGLRLAQIIATVMTgYADRPALGqrATELVTDPATGRTtlrllprfetitYAELWERVGAVAAAHDPEQPVRPG 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 98 DlreeRVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLAGQEHVELL------SPVVRKL-- 169
Cdd:cd17632 94 D----FVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEPRLLAVSAEHLDLAveavleGGTPPRLvv 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 170 ------------------------GVPLLPLPPTVYCGAAKDPGEEQVPERDwRDRGAMIIYTSGTTGRPKGVVITHHNV 225
Cdd:cd17632 170 fdhrpevdahraalesarerlaavGIPVTTLTLIAVRGRDLPPAPLFRPEPD-DDPLALLIYTSGSTGTPKGAMYTERLV 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 226 ---WATVTGLVHkwAWTKDDVILHVLPLHHVHGvVNKLLCPLWVGATCVMLPEFSAQLVWEKFLSSqspRVNVFMAVPTI 302
Cdd:cd17632 249 atfWLKVSSIQD--IRPPASITLNFMPMSHIAG-RISLYGTLARGGTAYFAAASDMSTLFDDLALV---RPTELFLVPRV 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 303 YSKLMDYH----DRHFGQPHVRDF----VRAVCEEKI---RLM--VSGSAALPLPVLEKWKGATGHTLLERYGMTEIGMA 369
Cdd:cd17632 323 CDMLFQRYqaelDRRSVAGADAETlaerVKAELRERVlggRLLaaVCGSAPLSAEMKAFMESLLDLDLHDGYGSTEAGAV 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 370 LSNplatarlpGSVGTPlPGVEVRIVSenpqkdgCPYIihaeGNETETRVTPgfeetEGELLVRGPSVFRGYWDKPEETK 449
Cdd:cd17632 403 ILD--------GVIVRP-PVLDYKLVD-------VPEL----GYFRTDRPHP-----RGELLVKTDTLFPGYYKRPEVTA 457
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 450 NAFTSDGWFKTGD--TAVFKDGSYWIRGRTSVDIIKTGGY-KVSALEVerLLLGHPSIADVAVIG-----------VPdv 515
Cdd:cd17632 458 EVFDEDGFYRTGDvmAELGPDRLVYVDRRNNVLKLSQGEFvTVARLEA--VFAASPLVRQIFVYGnserayllavvVP-- 533
|
570 580 590 600
....*....|....*....|....*....|....*....|....*.
gi 743747175 516 twGQRVTAVVTLREGHSLSHRELKEWARGV-LAPYAVPSELLLVEE 560
Cdd:cd17632 534 --TQDALAGEDTARLRAALAESLQRIAREAgLQSYEIPRDFLIETE 577
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
194-579 |
2.22e-15 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 79.01 E-value: 2.22e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 194 PERDWRDRgAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHH----VHGVVNKLLcplwVGAT 269
Cdd:cd05939 99 DDVNFRDK-LFYIYTSGTTGLPKAAVIVHSRYYRIAAGAYYAFGMRPEDVVYDCLPLYHsaggIMGVGQALL----HGST 173
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 270 CVMLPEFSAQLVWE-----------------KFLSSQSPRV-----NVFMAV-----PTIYSklmDYHDRhFGQPHVRDF 322
Cdd:cd05939 174 VVIRKKFSASNFWDdcvkynctivqyigeicRYLLAQPPSEeeqkhNVRLAVgnglrPQIWE---QFVRR-FGIPQIGEF 249
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 323 vravceekirlmvsgsaalplpvlekwkgatghtllerYGMTEIGMALSNPLATARLPGSVGTPLPGV-EVRIVSENPQ- 400
Cdd:cd05939 250 --------------------------------------YGATEGNSSLVNIDNHVGACGFNSRILPSVyPIRLIKVDEDt 291
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 401 ------KDG-CpyiIHAEGNETETRVtpgfeeteGELLVRGPSV-FRGYWDKPEETK----NAFTS-DGWFKTGDTAVFK 467
Cdd:cd05939 292 gelirdSDGlC---IPCQPGEPGLLV--------GKIIQNDPLRrFDGYVNEGATNKkiarDVFKKgDSAFLSGDVLVMD 360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 468 D-GSYWIRGRTSvDIIKTGGYKVSALEVERLLLGHPSIADVAVIGV--PDVTWGQRVTAVVTLREGHSLSH--RELkewa 542
Cdd:cd05939 361 ElGYLYFKDRTG-DTFRWKGENVSTTEVEGILSNVLGLEDVVVYGVevPGVEGRAGMAAIVDPERKVDLDRfsAVL---- 435
|
410 420 430
....*....|....*....|....*....|....*..
gi 743747175 543 RGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQLY 579
Cdd:cd05939 436 AKSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQKEGY 472
|
|
| PTZ00342 |
PTZ00342 |
acyl-CoA synthetase; Provisional |
205-470 |
9.08e-15 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240370 [Multi-domain] Cd Length: 746 Bit Score: 77.45 E-value: 9.08e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITHHNVWATVTGLvHKWAWTKD---DVILHVLPLHHVHGVVNKLLCpLWVGATCVmlpefsaqlV 281
Cdd:PTZ00342 309 IVYTSGTSGKPKGVMLSNKNLYNTVVPL-CKHSIFKKynpKTHLSYLPISHIYERVIAYLS-FMLGGTIN---------I 377
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 282 WEKFLSSQS-----PRVNVFMAVPT----IYSKLM----------------------DYHDRHFGQ-----PHVRDFVRA 325
Cdd:PTZ00342 378 WSKDINYFSkdiynSKGNILAGVPKvfnrIYTNIMteinnlpplkrflvkkilslrkSNNNGGFSKflegiTHISSKIKD 457
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 326 VCEEKIRLMVSGSAALPLPVLEKWKGATGHTLLERYGMTEIGMALSNPLATARLPGSVGTPL-PGVEVRIVSENPQK--D 402
Cdd:PTZ00342 458 KVNPNLEVILNGGGKLSPKIAEELSVLLNVNYYQGYGLTETTGPIFVQHADDNNTESIGGPIsPNTKYKVRTWETYKatD 537
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 743747175 403 GCPyiihaegnetetrvtpgfeetEGELLVRGPSVFRGYWDKPEETKNAFTSDGWFKTGDTA-VFKDGS 470
Cdd:PTZ00342 538 TLP---------------------KGELLIKSDSIFSGYFLEKEQTKNAFTEDGYFKTGDIVqINKNGS 585
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
121-586 |
2.44e-14 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 76.75 E-value: 2.44e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 121 SWMSGGTAVPLYRKHPQAELEYFIQDSRSSVVLA-------GQEHVELLSPVVR-KLGVPLLPLPPTVycgAAKDPGEEQ 192
Cdd:PRK05691 3790 SFKAGAGYLPLDPGLPAQRLQRIIELSRTPVLVCsaacreqARALLDELGCANRpRLLVWEEVQAGEV---ASHNPGIYS 3866
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 193 VPerdwrDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWvGATCVM 272
Cdd:PRK05691 3867 GP-----DNLAYVIYTSGSTGLPKGVMVEQRGMLNNQLSKVPYLALSEADVIAQTASQSFDISVWQFLAAPLF-GARVEI 3940
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 273 LPEFSAQLVWEKFLSSQSPRVNVFMAVPTIYSKLMdyhdrhfGQPHVrdfvravCEEKIRLMVSGSAALPLPVLEKWkga 352
Cdd:PRK05691 3941 VPNAIAHDPQGLLAHVQAQGITVLESVPSLIQGML-------AEDRQ-------ALDGLRWMLPTGEAMPPELARQW--- 4003
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 353 tghtlLERYgmTEIGMA-------LSNPLATAR--LPGSVGTPLPgvevrIVSenPQKDGCPYIIhaegNETETRVTPGf 423
Cdd:PRK05691 4004 -----LQRY--PQIGLVnaygpaeCSDDVAFFRvdLASTRGSYLP-----IGS--PTDNNRLYLL----DEALELVPLG- 4064
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 424 eeTEGELLVRGPSVFRGYWDKPEETKNAFTSDGW-------FKTGDTA-VFKDGSYWIRGRtsVD-IIKTGGYKVSALEV 494
Cdd:PRK05691 4065 --AVGELCVAGTGVGRGYVGDPLRTALAFVPHPFgapgerlYRTGDLArRRSDGVLEYVGR--IDhQVKIRGYRIELGEI 4140
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 495 ERLLLGHPSIADVAViGVPDVTWGQRVTAVVTLREGhSLSHREL----KEWARGVLAPYAVPSELLLVEEIPRNQMGKVN 570
Cdd:PRK05691 4141 EARLHEQAEVREAAV-AVQEGVNGKHLVGYLVPHQT-VLAQGALleriKQRLRAELPDYMVPLHWLWLDRLPLNANGKLD 4218
|
490 500
....*....|....*....|
gi 743747175 571 KRDL----VQQLYPQDRGTP 586
Cdd:PRK05691 4219 RKALpaldIGQLQSQAYLAP 4238
|
|
| PRK07769 |
PRK07769 |
long-chain-fatty-acid--CoA ligase; Validated |
100-476 |
7.39e-14 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 181109 [Multi-domain] Cd Length: 631 Bit Score: 74.38 E-value: 7.39e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 100 REERVSFLCSNDVSYVVAQWASWMSGGTAVPLYrkHPQA-----ELEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPLL 174
Cdd:PRK07769 78 PGDRVAILAPQNLDYLIAFFGALYAGRIAVPLF--DPAEpghvgRLHAVLDDCTPSAILTTTDSAEGVRKFFRARPAKER 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 175 PLPPTVycGAAKDP-GEEQVPERDWRDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKDDVILHVLPLHHV 253
Cdd:PRK07769 156 PRVIAV--DAVPDEvGATWVPPEANEDTIAYLQYTSGSTRIPAGVQITHLNLPTNVLQVIDALEGQEGDRGVSWLPFFHD 233
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 254 HGVVNKLLCPLWVGATCVMLPefsAQLV-----WEKFLSSQSP-RVNVFMAVPtiysklmdyhdrHFGQPHVRdfVRAVC 327
Cdd:PRK07769 234 MGLITVLLPALLGHYITFMSP---AAFVrrpgrWIRELARKPGgTGGTFSAAP------------NFAFEHAA--ARGLP 296
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 328 EE--------KIRLMVSGSAALPLPVLEKWKGAtghtlLERYGMTEIGMALSNPLATARLpgSVGTPLPGVEVRIV---- 395
Cdd:PRK07769 297 KDgeppldlsNVKGLLNGSEPVSPASMRKFNEA-----FAPYGLPPTAIKPSYGMAEATL--FVSTTPMDEEPTVIyvdr 369
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 396 --------------SEN--PQKdGCPYIIHAE-----GNETETRVTPGfeeTEGELLVRGPSVFRGYWDKPEETKNAF-- 452
Cdd:PRK07769 370 delnagrfvevpadAPNavAQV-SAGKVGVSEwavivDPETASELPDG---QIGEIWLHGNNIGTGYWGKPEETAATFqn 445
|
410 420 430
....*....|....*....|....*....|....*....
gi 743747175 453 -------------TSDG--WFKTGDTAVFKDGSYWIRGR 476
Cdd:PRK07769 446 ilksrlseshaegAPDDalWVRTGDYGVYFDGELYITGR 484
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
205-575 |
8.75e-12 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 68.05 E-value: 8.75e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVV--ITHHNVwATVTGLVHKWA-------WTKDDVILHVLPLHHVHGvvnkllcPLWVGATCVM--- 272
Cdd:PRK10524 238 ILYTSGTTGKPKGVQrdTGGYAV-ALATSMDTIFGgkagetfFCASDIGWVVGHSYIVYA-------PLLAGMATIMyeg 309
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 273 LPEFSAQLVW----EKFlssqspRVNVFMAVPTIYsKLMDYHDRHFGQPHVRDFVRAvceekirLMVSGSaalPL--PVL 346
Cdd:PRK10524 310 LPTRPDAGIWwrivEKY------KVNRMFSAPTAI-RVLKKQDPALLRKHDLSSLRA-------LFLAGE---PLdePTA 372
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 347 EKWKGATGHTLLERYGMTEIGM-ALSNPLATARLP---GSVGTPLPGVEVRIVSEnpqkdgcpyiihAEGNETEtrvtPG 422
Cdd:PRK10524 373 SWISEALGVPVIDNYWQTETGWpILAIARGVEDRPtrlGSPGVPMYGYNVKLLNE------------VTGEPCG----PN 436
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 423 feeTEGELLVRGP-------SVFRgywDKPEETKNAFTSDG--WFKTGDTAVF-KDGSYWIRGRTSvDIIKTGGYKVSAL 492
Cdd:PRK10524 437 ---EKGVLVIEGPlppgcmqTVWG---DDDRFVKTYWSLFGrqVYSTFDWGIRdADGYYFILGRTD-DVINVAGHRLGTR 509
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 493 EVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHREL-----KEWARGV---LAPYAVPSELLLVEEIPRN 564
Cdd:PRK10524 510 EIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPKDSDSLADREArlaleKEIMALVdsqLGAVARPARVWFVSALPKT 589
|
410
....*....|.
gi 743747175 565 QMGKVNKRDLV 575
Cdd:PRK10524 590 RSGKLLRRAIQ 600
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
100-476 |
9.33e-12 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 67.84 E-value: 9.33e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 100 REERVSFLCSNDVSYVVAQWASWMSGGTAVPLYRKHPQ--AE-LEYFIQDSRSSVVLAGQEHVELLSPVVRKLGVPLLPL 176
Cdd:PRK12476 91 PGDRVAILAPQGIDYVAGFFAAIKAGTIAVPLFAPELPghAErLDTALRDAEPTVVLTTTAAAEAVEGFLRNLPRLRRPR 170
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 177 PPTVycGAAKDP-GEEQVPERDWRDRGAMIIYTSGTTGRPKGVVITHHNVWATVTGLV----------HKWAWtkddvil 245
Cdd:PRK12476 171 VIAI--DAIPDSaGESFVPVELDTDDVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMIlsidlldrntHGVSW------- 241
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 246 hvLPLHHVHGVVNKLLCPLWVGATCVMLPefsAQLV-----WEKFLSSQSPRVNVFMAVPTIYSKLMdyhdRHFGQPHVR 320
Cdd:PRK12476 242 --LPLYHDMGLSMIGFPAVYGGHSTLMSP---TAFVrrpqrWIKALSEGSRTGRVVTAAPNFAYEWA----AQRGLPAEG 312
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 321 DFV--RAVceekirLMVSGSAALPLPVLEKWKGATGHTLLER------YGMTEIGMALSNpLATARLPGSV---GTPL-P 388
Cdd:PRK12476 313 DDIdlSNV------VLIIGSEPVSIDAVTTFNKAFAPYGLPRtafkpsYGIAEATLFVAT-IAPDAEPSVVyldREQLgA 385
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 389 GVEVRIVSENPQ---KDGCPYIIHaegNETETRVTPGFEE-----TEGELLVRGPSVFRGYWDKPEETKNAF-------- 452
Cdd:PRK12476 386 GRAVRVAADAPNavaHVSCGQVAR---SQWAVIVDPDTGAelpdgEVGEIWLHGDNIGRGYWGRPEETERTFgaklqsrl 462
|
410 420 430
....*....|....*....|....*....|....
gi 743747175 453 ----------TSDGWFKTGDTAVFKDGSYWIRGR 476
Cdd:PRK12476 463 aegshadgaaDDGTWLRTGDLGVYLDGELYITGR 496
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
205-569 |
7.51e-09 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 58.60 E-value: 7.51e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVITHHnvwATVTGLVHKWAWTKDDVILHVLPLHH------VHGVVNKLLCplwVGATCVML----- 273
Cdd:PTZ00237 259 ILYTSGTTGNSKAVVRSNG---PHLVGLKYYWRSIIEKDIPTVVFSHSsigwvsFHGFLYGSLS---LGNTFVMFeggii 332
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 274 -PEFSAQLVWEKFlssQSPRVNVFMAVPTIYSKLMDY-------HDRH---------FGQPHVRDFVRAVCEEKIRLMVS 336
Cdd:PTZ00237 333 kNKHIEDDLWNTI---EKHKVTHTLTLPKTIRYLIKTdpeatiiRSKYdlsnlkeiwCGGEVIEESIPEYIENKLKIKSS 409
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 337 GSaalplpvlekwkgatghtllerYGMTEIGMALSNPLATARLP-GSVGTPLPGVEVRIVSEnpqkDGcpyiihAEGNET 415
Cdd:PTZ00237 410 RG----------------------YGQTEIGITYLYCYGHINIPyNATGVPSIFIKPSILSE----DG------KELNVN 457
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 416 ETrvtpgfeeteGEL---LVRGPSVFRGYWDKPEETKNAFTS-DGWFKTGDTAvFKD--GSYWIRGRtSVDIIKTGGYKV 489
Cdd:PTZ00237 458 EI----------GEVafkLPMPPSFATTFYKNDEKFKQLFSKfPGYYNSGDLG-FKDenGYYTIVSR-SDDQIKISGNKV 525
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 490 SALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSHRELKEWARGV-------LAPYAVPSELLLVEEIP 562
Cdd:PTZ00237 526 QLNTIETSILKHPLVLECCSIGIYDPDCYNVPIGLLVLKQDQSNQSIDLNKLKNEInniitqdIESLAVLRKIIIVNQLP 605
|
....*..
gi 743747175 563 RNQMGKV 569
Cdd:PTZ00237 606 KTKTGKI 612
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
208-512 |
9.62e-09 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 57.85 E-value: 9.62e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 208 TSGTTGRPKGVVITHHNVWATVTGLVHKWAWTKD-DVILHVLPLHHVHGVVNkLLCPLWVGATCVMLPE--FSAQ-LVWE 283
Cdd:PRK05851 160 TAGSTGTPRTAILSPGAVLSNLRGLNARVGLDAAtDVGCSWLPLYHDMGLAF-LLTAALAGAPLWLAPTtaFSASpFRWL 238
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 284 KFLS-SQSprvnVFMAVPTI-YSKLMDYHDRhfgqphvrdfVRAVCEEKIRLMVSGSAALPLPVLEKWKGATGH------ 355
Cdd:PRK05851 239 SWLSdSRA----TLTAAPNFaYNLIGKYARR----------VSDVDLGALRVALNGGEPVDCDGFERFATAMAPfgfdag 304
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 356 TLLERYGMTEIGMALSNPL---------------ATARLPGSVGTPLPGVEVRIvsenpqkdgcpyiihaegNETETRVT 420
Cdd:PRK05851 305 AAAPSYGLAESTCAVTVPVpgiglrvdevttddgSGARRHAVLGNPIPGMEVRI------------------SPGDGAAG 366
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 421 PGFEETeGELLVRGPSVFRGYWDKPeetknAFTSDGWFKTGDTAVFKDGSYWIRGRTSvDIIKTGGYKVSALEVERLLLG 500
Cdd:PRK05851 367 VAGREI-GEIEIRGASMMSGYLGQA-----PIDPDDWFPTGDLGYLVDGGLVVCGRAK-ELITVAGRNIFPTEIERVAAQ 439
|
330
....*....|..
gi 743747175 501 HPSIADVAVIGV 512
Cdd:PRK05851 440 VRGVREGAVVAV 451
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
205-568 |
5.46e-06 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 49.19 E-value: 5.46e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 205 IIYTSGTTGRPKGVVithHNVWATVtgLVHkwawtkddvilhvLPLHHVHGVV-----------------NKLLCPLWVG 267
Cdd:cd05943 254 ILYSSGTTGLPKCIV---HGAGGTL--LQH-------------LKEHILHCDLrpgdrlfyyttcgwmmwNWLVSGLAVG 315
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 268 ATCVML---PEFSAQLV-WEkfLSSQSpRVNVFMAVPTIYSKLMDyhdRHFGQPHVRDFvravceEKIRLMVS-GSaalP 342
Cdd:cd05943 316 ATIVLYdgsPFYPDTNAlWD--LADEE-GITVFGTSAKYLDALEK---AGLKPAETHDL------SSLRTILStGS---P 380
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 343 LP------VLEKWKGatgHTLLERY-GMTEI--GMALSNPLATARlPGSVGTPLPGVEVRIVSEnpqkdgcpyiihaEGN 413
Cdd:cd05943 381 LKpesfdyVYDHIKP---DVLLASIsGGTDIisCFVGGNPLLPVY-RGEIQCRGLGMAVEAFDE-------------EGK 443
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 414 EtetrVTpgfEETeGELLVRG--PSVFRGYWDKPEETK--NAF--TSDGWFKTGDTAVF-KDGSYWIRGRtSVDIIKTGG 486
Cdd:cd05943 444 P----VW---GEK-GELVCTKpfPSMPVGFWNDPDGSRyrAAYfaKYPGVWAHGDWIEItPRGGVVILGR-SDGTLNPGG 514
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 487 YKVSALEVERLLLGHPSIADVAVIGVPDVTWGQRVTAVVTLREGHSLSH---RELKEWARGVLAPYAVPSELLLVEEIPR 563
Cdd:cd05943 515 VRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDERVILFVKLREGVELDDelrKRIRSTIRSALSPRHVPAKIIAVPDIPR 594
|
....*
gi 743747175 564 NQMGK 568
Cdd:cd05943 595 TLSGK 599
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
469-578 |
5.49e-05 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 45.96 E-value: 5.49e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 469 GSYWIRGRTSvDIIKTGGYKVSALEVERLLL-GHPSIADVAVIGVPDVTWG-QRVTAVVT---LREGHSLS-----HREL 538
Cdd:PLN03051 371 GYFCVQGRAD-DTMNLGGIKTSSVEIERACDrAVAGIAETAAVGVAPPDGGpELLVIFLVlgeEKKGFDQArpealQKKF 449
|
90 100 110 120
....*....|....*....|....*....|....*....|
gi 743747175 539 KEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQL 578
Cdd:PLN03051 450 QEAIQTNLNPLFKVSRVKIVPELPRNASNKLLRRVLRDQL 489
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
466-582 |
1.85e-03 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 41.22 E-value: 1.85e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 743747175 466 FKDGSYWIRGRTSvDIIKTGGYKVSALEVERLL-LGHPSIADVAVIGVPDVTWGQR---VTAVVTLREGHSLSHRELKE- 540
Cdd:PLN03052 600 TSGGYYRAHGRAD-DTMNLGGIKVSSVEIERVCnAADESVLETAAIGVPPPGGGPEqlvIAAVLKDPPGSNPDLNELKKi 678
|
90 100 110 120
....*....|....*....|....*....|....*....|....*
gi 743747175 541 WARGV---LAPYAVPSELLLVEEIPRNQMGKVNKRDLVQQLYPQD 582
Cdd:PLN03052 679 FNSAIqkkLNPLFKVSAVVIVPSFPRTASNKVMRRVLRQQLAQEL 723
|
|
| |
