Antagonist of mitotic exit network protein 1; Amn1 has been functionally characterized in Saccharomyces cerevisiae as a component of the Antagonist of MEN pathway (AMEN). The AMEN network is activated by MEN (mitotic exit network) via an active Cdc14, and in turn switches off MEN. Amn1 constitutes one of the alternative mechanisms by which MEN may be disrupted. Specifically, Amn1 binds Tem1 (Termination of M-phase, a GTPase that belongs to the RAS superfamily), and disrupts its association with Cdc15, the primary downstream target. Amn1 is a leucine-rich repeat (LRR) protein, with 12 repeats in the S. cerevisiae ortholog. As a negative regulator of the signal transduction pathway MEN, overexpression of AMN1 slows the growth of wild type cells. The function of the vertebrate members of this family has not been determined experimentally, they have fewer LRRs that determine the extent of this model.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 720089518 400 NQIRTLKLAWNKCLSDTTLKKVGIVCPNLRSLDLSHCWSITEKGIEAIGSSCigimelkvnGCKEMANLGTTMGfskles 479
Cdd:cd09293   52 NKLKKLILPGSKLIDDEGLIALAQSCPNLQVLDLRACENITDSGIVALATNC---------PKLQTINLGRHRN------ 116

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 720089518 480 lraaGSGMNDEGLSMVSQSCRRLLCLDLEGClEVTAKGVKEMV-GNCKGLRELNLKKCCKVS 540
Cdd:cd09293  117 ----GHLITDVSLSALGKNCTFLQTVGFAGC-DVTDKGVWELAsGCSKSLERLSLNNCRNLT 173

F-box domain superfamily; This short domain is commonly found at the N-terminus of various proteins, and typically co-occurs with one or more other conserved domains or motifs, such as leucine rich repeats, WD40 repeats, kelch, tub, spry, and others. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression. One of the best researched roles of F-box proteins is their participation in SCF (Skp1-Cul1-F-box protein), a multi-protein complex that functions as a ubiquitin E3 ligase, where the role of the F-box protein is to recruit target substrates. Gene families containing the F-box are found greatly expanded in narrow taxonomic lineages, such as flowering plants and nematodes. In this hierarchical classification, many of the subfamilies are named according to their domain architectures.

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 720089518   7 DLPEECWESVFNRLDHHQHFEALSLVCKRFLSISNQLR 44
Cdd:cd22159    3 LLPDEILELIFSYLSDPWDRNSCSLVCKRWYRLERATR 40

Antagonist of mitotic exit network protein 1; Amn1 has been functionally characterized in Saccharomyces cerevisiae as a component of the Antagonist of MEN pathway (AMEN). The AMEN network is activated by MEN (mitotic exit network) via an active Cdc14, and in turn switches off MEN. Amn1 constitutes one of the alternative mechanisms by which MEN may be disrupted. Specifically, Amn1 binds Tem1 (Termination of M-phase, a GTPase that belongs to the RAS superfamily), and disrupts its association with Cdc15, the primary downstream target. Amn1 is a leucine-rich repeat (LRR) protein, with 12 repeats in the S. cerevisiae ortholog. As a negative regulator of the signal transduction pathway MEN, overexpression of AMN1 slows the growth of wild type cells. The function of the vertebrate members of this family has not been determined experimentally, they have fewer LRRs that determine the extent of this model.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 720089518 400 NQIRTLKLAWNKCLSDTTLKKVGIVCPNLRSLDLSHCWSITEKGIEAIGSSCigimelkvnGCKEMANLGTTMGfskles 479
Cdd:cd09293   52 NKLKKLILPGSKLIDDEGLIALAQSCPNLQVLDLRACENITDSGIVALATNC---------PKLQTINLGRHRN------ 116

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 720089518 480 lraaGSGMNDEGLSMVSQSCRRLLCLDLEGClEVTAKGVKEMV-GNCKGLRELNLKKCCKVS 540
Cdd:cd09293  117 ----GHLITDVSLSALGKNCTFLQTVGFAGC-DVTDKGVWELAsGCSKSLERLSLNNCRNLT 173

F-box domain found in Arabidopsis thaliana transport inhibitor response 1 protein (TIR1), coronatine-insensitive protein 1 (COI1) and similar proteins; TIR1, also called F-box/LRR-repeat protein 1 (FBL1), is part of an SCF (SKP1-cullin-F-box) protein ligase complex that promotes the ubiquitin-dependent proteolysis of a family of transcriptional regulators known as Aux/IAAs in an auxin-dependent manner. TIR1 is an auxin receptor that plays a potential role in plant hormone signaling. COI1, also called F-box/LRR-repeat protein 2 (FBL2), is the substrate-recruiting module of an SCF ubiquitin E3 ligase complex. It mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JASMONATE ZIM-domain (JAZ) family proteins. This subfamily also includes Arabidopsis thaliana EIN3-binding F-box protein 1 (EBF1). EBF1, also called F-box/LRR-repeat protein 6 (FBL6), is a component of the SCF(EBF1) E3 ubiquitin ligase complex, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including EIN3 and EIL1). The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 720089518   7 DLPEECWESVFNRLDHHQHFEALSLVCKRFLSISNQLR 44
Cdd:cd22159    3 LLPDEILELIFSYLSDPWDRNSCSLVCKRWYRLERATR 40

F-box domain; This domain is approximately 50 amino acids long, and is usually found in the N-terminal half of a variety of proteins. Two motifs that are commonly found associated with the F-box domain are the leucine rich repeats (LRRs; pfam00560 and pfam07723) and the WD repeat (pfam00400). The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 720089518    7 DLPEECWESVFNRLDHHQHFeALSLVCKRFLSISNQLRSRLT 48
Cdd:pfam00646   3 DLPDDLLLEILSRLDPKDLL-RLSLVSKRWRSLVDSLKLWKK 43

Antagonist of mitotic exit network protein 1; Amn1 has been functionally characterized in Saccharomyces cerevisiae as a component of the Antagonist of MEN pathway (AMEN). The AMEN network is activated by MEN (mitotic exit network) via an active Cdc14, and in turn switches off MEN. Amn1 constitutes one of the alternative mechanisms by which MEN may be disrupted. Specifically, Amn1 binds Tem1 (Termination of M-phase, a GTPase that belongs to the RAS superfamily), and disrupts its association with Cdc15, the primary downstream target. Amn1 is a leucine-rich repeat (LRR) protein, with 12 repeats in the S. cerevisiae ortholog. As a negative regulator of the signal transduction pathway MEN, overexpression of AMN1 slows the growth of wild type cells. The function of the vertebrate members of this family has not been determined experimentally, they have fewer LRRs that determine the extent of this model.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 720089518 400 NQIRTLKLAWNKCLSDTTLKKVGIVCPNLRSLDLSHCWSITEKGIEAIGSSCigimelkvnGCKEMANLGTTMGfskles 479
Cdd:cd09293   52 NKLKKLILPGSKLIDDEGLIALAQSCPNLQVLDLRACENITDSGIVALATNC---------PKLQTINLGRHRN------ 116

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 720089518 480 lraaGSGMNDEGLSMVSQSCRRLLCLDLEGClEVTAKGVKEMV-GNCKGLRELNLKKCCKVS 540
Cdd:cd09293  117 ----GHLITDVSLSALGKNCTFLQTVGFAGC-DVTDKGVWELAsGCSKSLERLSLNNCRNLT 173

Antagonist of mitotic exit network protein 1; Amn1 has been functionally characterized in Saccharomyces cerevisiae as a component of the Antagonist of MEN pathway (AMEN). The AMEN network is activated by MEN (mitotic exit network) via an active Cdc14, and in turn switches off MEN. Amn1 constitutes one of the alternative mechanisms by which MEN may be disrupted. Specifically, Amn1 binds Tem1 (Termination of M-phase, a GTPase that belongs to the RAS superfamily), and disrupts its association with Cdc15, the primary downstream target. Amn1 is a leucine-rich repeat (LRR) protein, with 12 repeats in the S. cerevisiae ortholog. As a negative regulator of the signal transduction pathway MEN, overexpression of AMN1 slows the growth of wild type cells. The function of the vertebrate members of this family has not been determined experimentally, they have fewer LRRs that determine the extent of this model.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 720089518 318 LLSAYRSLNYLDIGGADFLTDQSITELFQYLQNLSFINLNSCSHLTNSTLFNLARDCPSLEEI---RMERTSLGEEKFST 394
Cdd:cd09293   47 QLSNCNKLKKLILPGSKLIDDEGLIALAQSCPNLQVLDLRACENITDSGIVALATNCPKLQTInlgRHRNGHLITDVSLS 126

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 720089518 395 ELRKNN-QIRTLKLAWNKcLSDTTLKKVGIVC-PNLRSLDLSHCWSITEKGIEAI----GSSCIGIMEL 457
Cdd:cd09293  127 ALGKNCtFLQTVGFAGCD-VTDKGVWELASGCsKSLERLSLNNCRNLTDQSIPAIlasnYFPNLSVLEF 194

F-box domain found in Arabidopsis thaliana transport inhibitor response 1 protein (TIR1), coronatine-insensitive protein 1 (COI1) and similar proteins; TIR1, also called F-box/LRR-repeat protein 1 (FBL1), is part of an SCF (SKP1-cullin-F-box) protein ligase complex that promotes the ubiquitin-dependent proteolysis of a family of transcriptional regulators known as Aux/IAAs in an auxin-dependent manner. TIR1 is an auxin receptor that plays a potential role in plant hormone signaling. COI1, also called F-box/LRR-repeat protein 2 (FBL2), is the substrate-recruiting module of an SCF ubiquitin E3 ligase complex. It mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JASMONATE ZIM-domain (JAZ) family proteins. This subfamily also includes Arabidopsis thaliana EIN3-binding F-box protein 1 (EBF1). EBF1, also called F-box/LRR-repeat protein 6 (FBL6), is a component of the SCF(EBF1) E3 ubiquitin ligase complex, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including EIN3 and EIL1). The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 720089518   7 DLPEECWESVFNRLDHHQHFEALSLVCKRFLSISNQLR 44
Cdd:cd22159    3 LLPDEILELIFSYLSDPWDRNSCSLVCKRWYRLERATR 40

F-box domain; This domain is approximately 50 amino acids long, and is usually found in the N-terminal half of a variety of proteins. Two motifs that are commonly found associated with the F-box domain are the leucine rich repeats (LRRs; pfam00560 and pfam07723) and the WD repeat (pfam00400). The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 720089518    7 DLPEECWESVFNRLDHHQHFeALSLVCKRFLSISNQLRSRLT 48
Cdd:pfam00646   3 DLPDDLLLEILSRLDPKDLL-RLSLVSKRWRSLVDSLKLWKK 43