|
Name |
Accession |
Description |
Interval |
E-value |
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
64-582 |
0e+00 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 684.02 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEICRALDcssrDLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:cd05941 1 DRIAIVDDGDSITYADLVARAARLANRLLALGK----DLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAEL 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIaaeeyvgkitpsaeklgvpvlpllrscsdgsashpaveegplttcsswknRGAMIIYTSGTTGRPKG 223
Cdd:cd05941 77 EYVITDSEPSLVL--------------------------------------------------DPALILYTSGTTGRPKG 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 224 VLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWkklLSSQAPRISVFM 303
Cdd:cd05941 107 VVLTHANLAANVRALVDAWRWTEDDVLLHVLPLHHVHGLVNALLCPLFAGASVEFLPKFDPKEVA---ISRLMPSITVFM 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 304 AVPTIYAKLVEYYDKHFFQPQvqdFVRAFCQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMALSNPLHGV 383
Cdd:cd05941 184 GVPTIYTRLLQYYEAHFTDPQ---FARAAAAERLRLMVSGSAALPVPTLEEWEAITGHTLLERYGMTEIGMALSNPLDGE 260
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 384 RIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQvtPGLEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGW 463
Cdd:cd05941 261 RRPGTVGMPLPGVQARIV-----------------DEETGE--PLPRGEVGEIQVRGPSVFKEYWNKPEATKEEFTDDGW 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 464 FKTGDTAAYK-DGVYWIKGRTSVDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKG-EMLSV 541
Cdd:cd05941 322 FKTGDLGVVDeDGYYWILGRSSVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRAGaAALSL 401
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 2452540125 542 KDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQ 582
Cdd:cd05941 402 EELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
59-584 |
4.49e-139 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 411.51 E-value: 4.49e-139
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcSSRDLKE-ERISFLCPNDASYVVAQWASWMSGGIAVPLYKK 137
Cdd:COG0318 9 AARHPDRPALVFGGRRLTYAELDARARRLA----AAL--RALGVGPgDRVALLLPNSPEFVVAFLAALRAGAVVVPLNPR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 138 HPVTELEYVIQDSQSALVIAAeeyvgkitpsaeklgvpvlpllrscsdgsashpaveegplttcsswknrgaMIIYTSGT 217
Cdd:COG0318 83 LTAEELAYILEDSGARALVTA---------------------------------------------------LILYTSGT 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 218 TGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWKKLlssQAP 297
Cdd:COG0318 112 TGRPKGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPLLAGATLVLLPRFDPERVLELI---ERE 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 298 RISVFMAVPTIYAKLVEYYDKHFFqpqvqDFvrafcqENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMALS 377
Cdd:COG0318 189 RVTVLFGVPTMLARLLRHPEFARY-----DL------SSLRLVVSGGAPLPPELLERFEERFGVRIVEGYGLTETSPVVT 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 378 -NPL-HGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFREYWNRPRETE 455
Cdd:COG0318 258 vNPEdPGERRPGSVGRPLPGVEVRIV-----------------DEDGRELPPG---EVGEIVVRGPNVMKGYWNDPEATA 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 456 DAFtPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLR 534
Cdd:COG0318 318 EAF-RDGWLRTGDLGRLdEDGYLYIVGRKK-DMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVLR 395
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 2452540125 535 KGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQRF 584
Cdd:COG0318 396 PGAELDAEELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRERY 445
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
52-580 |
2.92e-112 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 343.39 E-value: 2.92e-112
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 52 VAPVFTKALA-FGDKIAIIDQNGEHTYRDLLsqslRLSQEICRALDCSsrDLKE-ERISFLCPNDASYVVAQWASWMSGG 129
Cdd:cd05936 1 LADLLEEAARrFPDKTALIFMGRKLTYRELD----ALAEAFAAGLQNL--GVQPgDRVALMLPNCPQFPIAYFGALKAGA 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 130 IAV---PLYKKHpvtELEYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLPllrscsdgsASHPAVeegplttcsswkn 206
Cdd:cd05936 75 VVVplnPLYTPR---ELEHILNDSGAKALIVAVSFTDLLAAGAPLGERVALT---------PEDVAV------------- 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 207 rgamIIYTSGTTGRPKGVLSTHEN-------VQAVTTGLVEkwewkKEDVILHVLPLHHVHGVINKLLCPLWVGATCVML 279
Cdd:cd05936 130 ----LQYTSGTTGVPKGAMLTHRNlvanalqIKAWLEDLLE-----GDDVVLAALPLFHVFGLTVALLLPLALGATIVLI 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 280 PEFNAQMVWKKLlssQAPRISVFMAVPTIYAKLVEYYDKHFFQPQvqdfvrafcqeNIRLMVSGSAALPVPVLEKWKSIT 359
Cdd:cd05936 201 PRFRPIGVLKEI---RKHRVTIFPGVPTMYIALLNAPEFKKRDFS-----------SLRLCISGGAPLPVEVAERFEELT 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 360 GHTLLERYGMTEIG-MALSNPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPgleGQEGELLV 438
Cdd:cd05936 267 GVPIVEGYGLTETSpVVAVNPLDGPRKPGSIGIPLPGTEVKIV-----------------DDDGEELPP---GEVGELWV 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 439 RGPSVFREYWNRPRETEDAFTpDGWFKTGDTaAY--KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVI 516
Cdd:cd05936 327 RGPQVMKGYWNRPEETAEAFV-DGWLRTGDI-GYmdEDGYFFIVDRKK-DMIIVGGFNVYPREVEEVLYEHPAVAEAAVV 403
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2452540125 517 GPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05936 404 GVPDPYSGEAVKAFVVLKEGASLTEEEIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRREL 467
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
56-584 |
5.78e-105 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 325.68 E-value: 5.78e-105
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 56 FTKALAFGDKIAIIDQNGEH-TYRDLLSQSLRLSqeicRALDcsSRDLKE-ERISFLCPNDASYVVAQWASWMSGGIAVP 133
Cdd:PRK07514 9 LRAAFADRDAPFIETPDGLRyTYGDLDAASARLA----NLLV--ALGVKPgDRVAVQVEKSPEALALYLATLRAGAVFLP 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 134 LYKKHPVTELEYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVL---------PLLRSCSDGSASHPAVEEGP--Lttcs 202
Cdd:PRK07514 83 LNTAYTLAELDYFIGDAEPALVVCDPANFAWLSKIAAAAGAPHVetldadgtgSLLEAAAAAPDDFETVPRGAddL---- 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 203 swknrgAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEF 282
Cdd:PRK07514 159 ------AAILYTSGTTGRSKGAMLSHGNLLSNALTLVDYWRFTPDDVLIHALPIFHTHGLFVATNVALLAGASMIFLPKF 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 283 NAQMVWKKLlssqaPRISVFMAVPTIYAKLVeyydkhffqpQVQDFVRAFCQeNIRLMVSGSAALPVPVLEKWKSITGHT 362
Cdd:PRK07514 233 DPDAVLALM-----PRATVMMGVPTFYTRLL----------QEPRLTREAAA-HMRLFISGSAPLLAETHREFQERTGHA 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 363 LLERYGMTEIGMALSNPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPglEGQEGELLVRGPS 442
Cdd:PRK07514 297 ILERYGMTETNMNTSNPYDGERRAGTVGFPLPGVSLRVT-----------------DPETGAELP--PGEIGMIEVKGPN 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 443 VFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDM 521
Cdd:PRK07514 358 VFKGYWRMPEKTAEEFRADGFFITGDLGKIdERGYVHIVGR-GKDLIISGGYNVYPKEVEGEIDELPGVVESAVIGVPHP 436
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2452540125 522 VWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQRF 584
Cdd:PRK07514 437 DFGEGVTAVVVPKPGAALDEAAILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLLREQY 499
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
106-582 |
9.27e-105 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 323.87 E-value: 9.27e-105
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 106 RISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALVIAAeeyvgkitPSAEKLGVPVLPL-LRSCS 184
Cdd:PRK07787 47 RVAVLATPTLATVLAVVGALIAGVPVVPVPPDSGVAERRHILADSGAQAWLGP--------APDDPAGLPHVPVrLHARS 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 185 DGSASHPAvEEGPlttcsswknrgAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVIN 264
Cdd:PRK07787 119 WHRYPEPD-PDAP-----------ALIVYTSGTTGPPKGVVLSRRAIAADLDALAEAWQWTADDVLVHGLPLFHVHGLVL 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 265 KLLCPLWVGATCVMLPEFNAQMVWKKLlssqAPRISVFMAVPTIYAKLVEyydkhffqpqVQDFVRAFcqENIRLMVSGS 344
Cdd:PRK07787 187 GVLGPLRIGNRFVHTGRPTPEAYAQAL----SEGGTLYFGVPTVWSRIAA----------DPEAARAL--RGARLLVSGS 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 345 AALPVPVLEKWKSITGHTLLERYGMTEIGMALSNPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQ 424
Cdd:PRK07787 251 AALPVPVFDRLAALTGHRPVERYGMTETLITLSTRADGERRPGWVGLPLAGVETRLV-----------------DEDGGP 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 425 VTpgLEGQE-GELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAYK-DGVYWIKGRTSVDIIKNGGFKISALEVER 502
Cdd:PRK07787 314 VP--HDGETvGELQVRGPTLFDGYLNRPDATAAAFTADGWFRTGDVAVVDpDGMHRIVGRESTDLIKSGGYRIGAGEIET 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 503 HLLAHPHITDVAVIGPPDMVWGQRVSAVVQlrKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQ 582
Cdd:PRK07787 392 ALLGHPGVREAAVVGVPDDDLGQRIVAYVV--GADDVAADELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLLS 469
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
209-575 |
4.25e-95 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 294.19 E-value: 4.25e-95
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVhGVINKLLCPLWVGATCVMLPEFNAQMVW 288
Cdd:cd04433 3 ALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLPKFDPEAAL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 KKLlssQAPRISVFMAVPTIYAKLVEYYDKHFFqpqvqDFVRafcqenIRLMVSGSAALPVPVLEKWKSITGHTLLERYG 368
Cdd:cd04433 82 ELI---EREKVTILLGVPTLLARLLKAPESAGY-----DLSS------LRALVSGGAPLPPELLERFEEAPGIKLVNGYG 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 369 MTEIG--MALSNPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFRE 446
Cdd:cd04433 148 LTETGgtVATGPPDDDARKPGSVGRPVPGVEVRIV-----------------DPDGGELPPG---EIGELVVRGPSVMKG 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 447 YWNRPRETEdAFTPDGWFKTGDtAAYKD--GVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWG 524
Cdd:cd04433 208 YWNNPEATA-AVDEDGWYRTGD-LGRLDedGYLYIVGRLK-DMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWG 284
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|.
gi 2452540125 525 QRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKV 575
Cdd:cd04433 285 ERVVAVVVLRPGADLDAEELRAHVRERLAPYKVPRRVVFVDALPRTASGKI 335
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
61-584 |
1.80e-94 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 299.02 E-value: 1.80e-94
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 61 AFGDKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPLYKK 137
Cdd:PRK06187 18 KHPDKEAVYFDGRRTTYAELDERVNRLA----NAL----RALgvkKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPINIR 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 138 HPVTELEYVIQDSQSALVIAAEEYVGKITPSAEKL-------------GVPVLPLLRSCSDGSASHPAVEEGPLTTcssw 204
Cdd:PRK06187 90 LKPEEIAYILNDAEDRVVLVDSEFVPLLAAILPQLptvrtvivegdgpAAPLAPEVGEYEELLAAASDTFDFPDID---- 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 205 KNRGAMIIYTSGTTGRPKGVLSTH----ENVQAVTTGLvekwEWKKEDVILHVLPLHHVHGvinkLLCP---LWVGATCV 277
Cdd:PRK06187 166 ENDAAAMLYTSGTTGHPKGVVLSHrnlfLHSLAVCAWL----KLSRDDVYLVIVPMFHVHA----WGLPylaLMAGAKQV 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 278 MLPEFNAQMVWKKLLSSqapRISVFMAVPTIYAKLVEYYDKHFfqpqvQDFVRafcqenIRLMVSGSAALPVPVLEKWKS 357
Cdd:PRK06187 238 IPRRFDPENLLDLIETE---RVTFFFAVPTIWQMLLKAPRAYF-----VDFSS------LRLVIYGGAALPPALLREFKE 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 358 ITGHTLLERYGMTEIGMALS-NPLH-----GVRIPGSVGTPLPGVEVCIatetlkdgarsytIHAQGDEsntqvTPGLEG 431
Cdd:PRK06187 304 KFGIDLVQGYGMTETSPVVSvLPPEdqlpgQWTKRRSAGRPLPGVEARI-------------VDDDGDE-----LPPDGG 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 432 QEGELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDtAAY--KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPH 509
Cdd:PRK06187 366 EVGEIIVRGPWLMQGYWNRPEATAETID-GGWLHTGD-VGYidEDGYLYITDRIK-DVIISGGENIYPRELEDALYGHPA 442
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2452540125 510 ITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQRF 584
Cdd:PRK06187 443 VAEVAVIGVPDEKWGERPVAVVVLKPGATLDAKELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVLREQY 517
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
63-577 |
1.25e-93 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 294.13 E-value: 1.25e-93
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 63 GDKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHP 139
Cdd:cd17631 9 PDRTALVFGGRSLTYAELDERVNRLA----HAL----RALgvaKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLT 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 140 VTELEYVIQDSQSALVIAAEeyvgkitpsaeklgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTG 219
Cdd:cd17631 81 PPEVAYILADSGAKVLFDDL-------------------------------------------------ALLMYTSGTTG 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 220 RPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWKKLlssQAPRI 299
Cdd:cd17631 112 RPKGAMLTHRNLLWNAVNALAALDLGPDDVLLVVAPLFHIGGLGVFTLPTLLRGGTVVILRKFDPETVLDLI---ERHRV 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 300 SVFMAVPTIYAKLVEYYDkhffqPQVQDFVRafcqenIRLMVSGSAALPVPVLEKWKSItGHTLLERYGMTEIGMALS-- 377
Cdd:cd17631 189 TSFFLVPTMIQALLQHPR-----FATTDLSS------LRAVIYGGAPMPERLLRALQAR-GVKFVQGYGMTETSPGVTfl 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 378 NPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFREYWNRPRETEDA 457
Cdd:cd17631 257 SPEDHRRKLGSAGRPVFFVEVRIV-----------------DPDGREVPPG---EVGEIVVRGPHVMAGYWNRPEATAAA 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 458 FTpDGWFKTGDtAAYKD--GVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRK 535
Cdd:cd17631 317 FR-DGWFHTGD-LGRLDedGYLYIVDRKK-DMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVVAVVVPRP 393
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 2452540125 536 GEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNK 577
Cdd:cd17631 394 GAELDEDELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
59-583 |
8.58e-91 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 289.11 E-value: 8.58e-91
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPLY 135
Cdd:PRK07656 15 ARRFGDKEAYVFGDQRLTYAELNARVRRAA----AAL----AALgigKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLN 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 136 KKHPVTELEYVIQDSQSALVIAAEEYVGKITPSAEKLgvPVLPLLRSCSDGsasHPAVEEGPLTTCSSWKNRG------- 208
Cdd:PRK07656 87 TRYTADEAAYILARGDAKALFVLGLFLGVDYSATTRL--PALEHVVICETE---EDDPHTEKMKTFTDFLAAGdpaerap 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 -------AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPE 281
Cdd:PRK07656 162 evdpddvADILFTSGTTGRPKGAMLTHRQLLSNAADWAEYLGLTEGDRYLAANPFFHVFGYKAGVNAPLMRGATILPLPV 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 282 FNAQMVWKKLlssQAPRISVFMAVPTIYAKLVEYYDKHFFqpqvqDFvrafcqENIRLMVSGSAALPVPVLEKWKSITG- 360
Cdd:PRK07656 242 FDPDEVFRLI---ETERITVLPGPPTMYNSLLQHPDRSAE-----DL------SSLRLAVTGAASMPVALLERFESELGv 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 361 HTLLERYGMTE-IGMALSNPLHGVR--IPGSVGTPLPGVEVCIATEtlkdgarsytihaQGDESNTqvtpgleGQEGELL 437
Cdd:PRK07656 308 DIVLTGYGLSEaSGVTTFNRLDDDRktVAGTIGTAIAGVENKIVNE-------------LGEEVPV-------GEVGELL 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 438 VRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVI 516
Cdd:PRK07656 368 VRGPNVMKGYYDDPEATAAAIDADGWLHTGDLGRLdEEGYLYIVDRKK-DMFIVGGFNVYPAEVEEVLYEHPAVAEAAVI 446
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 517 GPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQR 583
Cdd:PRK07656 447 GVPDERLGEVGKAYVVLKPGAELTEEELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALREK 513
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
64-582 |
2.87e-90 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 287.29 E-value: 2.87e-90
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLsqslRLSQEICRALdcSSRDLKE-ERISFLCPNDASYVVAQWASWMSGGIAVPL---YKKhp 139
Cdd:cd05926 4 PALVVPGSTPALTYADLA----ELVDDLARQL--AALGIKKgDRVAIALPNGLEFVVAFLAAARAGAVVAPLnpaYKK-- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 140 vTELEYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLPLlrsCSDGSASHPAVEEGPLTTCSSWK-----------NRG 208
Cdd:cd05926 76 -AEFEFYLADLGSKLVLTPKGELGPASRAASKLGLAILEL---ALDVGVLIRAPSAESLSNLLADKknaksegvplpDDL 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVW 288
Cdd:cd05926 152 ALILHTSGTTGRPKGVPLTHRNLAASATNITNTYKLTPDDRTLVVMPLFHVHGLVASLLSTLAAGGSVVLPPRFSASTFW 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 KKllsSQAPRISVFMAVPTIYAKLVEYYDKHFFQPQVQdfvrafcqenIRLMVSGSAALPVPVLEKWKSITGHTLLERYG 368
Cdd:cd05926 232 PD---VRDYNATWYTAVPTIHQILLNRPEPNPESPPPK----------LRFIRSCSASLPPAVLEALEATFGAPVLEAYG 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 369 MTEIG--MAlSNPL-HGVRIPGSVGTPLpGVEVCIATEtlkDGArsytihaqgdesntqvtPGLEGQEGELLVRGPSVFR 445
Cdd:cd05926 299 MTEAAhqMT-SNPLpPGPRKPGSVGKPV-GVEVRILDE---DGE-----------------ILPPGVVGEICLRGPNVTR 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 446 EYWNRPRETEDAFTPDGWFKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWG 524
Cdd:cd05926 357 GYLNNPEANAEAAFKDGWFRTGDLGYLDaDGYLFLTGRIK-ELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYG 435
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 2452540125 525 QRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQ 582
Cdd:cd05926 436 EEVAAAVVLREGASVTEEELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
55-491 |
2.65e-86 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 274.19 E-value: 2.65e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 55 VFTKALAFGDKIAIIDQNGEH-TYRDLLSQSLRLSqeicRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGI 130
Cdd:pfam00501 1 LERQAARTPDKTALEVGEGRRlTYRELDERANRLA----AGL----RALgvgKGDRVAILLPNSPEWVVAFLACLKAGAV 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 131 AVPLYKKHPVTELEYVIQDSQSALVIAAEEYvgKITPSAEKLGVPVLPLLRSCSDGsasHPAVEEGPLTTCSSWKNRG-- 208
Cdd:pfam00501 73 YVPLNPRLPAEELAYILEDSGAKVLITDDAL--KLEELLEALGKLEVVKLVLVLDR---DPVLKEEPLPEEAKPADVPpp 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 ----------AMIIYTSGTTGRPKGVLSTHENV--QAVTTGLVEKWEWK--KEDVILHVLPLHHVHGVINKLLCPLWVGA 274
Cdd:pfam00501 148 pppppdpddlAYIIYTSGTTGKPKGVMLTHRNLvaNVLSIKRVRPRGFGlgPDDRVLSTLPLFHDFGLSLGLLGPLLAGA 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 275 TCVMLPEFNAQMVWKKLLSSQAPRISVFMAVPTIYAKLVEyydkhffqpqvQDFVRAFCQENIRLMVSGSAALPVPVLEK 354
Cdd:pfam00501 228 TVVLPPGFPALDPAALLELIERYKVTVLYGVPTLLNMLLE-----------AGAPKRALLSSLRLVLSGGAPLPPELARR 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 355 WKSITGHTLLERYGMTEIGMALSNPLHGV---RIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPglEG 431
Cdd:pfam00501 297 FRELFGGALVNGYGLTETTGVVTTPLPLDedlRSLGSVGRPLPGTEVKIV-----------------DDETGEPVP--PG 357
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2452540125 432 QEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNG 491
Cdd:pfam00501 358 EPGELCVRGPGVMKGYLNDPELTAEAFDEDGWYRTGDLGRRdEDGYLEIVGRKK-DQIKLG 417
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
55-579 |
4.61e-82 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 268.02 E-value: 4.61e-82
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 55 VFTKALA-FGDKIAIIDQNGEHTYRDLLSQsLRLSQEICRALDCSSRDlkeeRISFLCPNDASYVVAQWASWMSGGIAV- 132
Cdd:PRK05605 37 LYDNAVArFGDRPALDFFGATTTYAELGKQ-VRRAAAGLRALGVRPGD----RVAIVLPNCPQHIVAFYAVLRLGAVVVe 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 133 --PLYKKHpvtELEYVIQDSQSALVIAAEeyvgKITPSAEKL--GVPV-----------------------LPLLRSCSD 185
Cdd:PRK05605 112 hnPLYTAH---ELEHPFEDHGARVAIVWD----KVAPTVERLrrTTPLetivsvnmiaampllqrlalrlpIPALRKARA 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 186 ------------------------GSASHPAVEEGPLttcsswknrgAMIIYTSGTTGRPKGVLSTHENV-------QAV 234
Cdd:PRK05605 185 altgpapgtvpwetlvdaaiggdgSDVSHPRPTPDDV----------ALILYTSGTTGKPKGAQLTHRNLfanaaqgKAW 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 235 TTGLVEKWEwkkedVILHVLPLHHVHGV-INKLLCPLwVGATCVMLPEFNAQMVwKKLLSSQAPrisVFM-AVPTIYAKL 312
Cdd:PRK05605 255 VPGLGDGPE-----RVLAALPMFHAYGLtLCLTLAVS-IGGELVLLPAPDIDLI-LDAMKKHPP---TWLpGVPPLYEKI 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 313 VEYYDKHffqpqvqdfvrAFCQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIG-MALSNPLHGVRIPGSVGT 391
Cdd:PRK05605 325 AEAAEER-----------GVDLSGVRNAFSGAMALPVSTVELWEKLTGGLLVEGYGLTETSpIIVGNPMSDDRRPGYVGV 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 392 PLPGVEVCIA-----TETLKDGarsytihaqgdesntqvtpglegQEGELLVRGPSVFREYWNRPRETEDAFTpDGWFKT 466
Cdd:PRK05605 394 PFPDTEVRIVdpedpDETMPDG-----------------------EEGELLVRGPQVFKGYWNRPEETAKSFL-DGWFRT 449
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 467 GDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLK 545
Cdd:PRK05605 450 GDVVVMEeDGFIRIVDRIK-ELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAALDPEGLR 528
|
570 580 590
....*....|....*....|....*....|....
gi 2452540125 546 DWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKE 579
Cdd:PRK05605 529 AYCREHLTRYKVPRRFYHVDELPRDQLGKVRRRE 562
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
63-575 |
8.79e-82 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 266.98 E-value: 8.79e-82
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 63 GDKIAII--DQNGEH---TYRDLLSQSLRLSQeicrALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPL 134
Cdd:COG0365 23 GDKVALIweGEDGEErtlTYAELRREVNRFAN----AL----RALgvkKGDRVAIYLPNIPEAVIAMLACARIGAVHSPV 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 135 YKKHPVTELEYVIQDSQSALVIAAEEYV--GKITPSAEKLG--VPVLPLLRSC----------------------SDGSA 188
Cdd:COG0365 95 FPGFGAEALADRIEDAEAKVLITADGGLrgGKVIDLKEKVDeaLEELPSLEHVivvgrtgadvpmegdldwdellAAASA 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 189 SHPAVE---EGPLttcsswknrgaMIIYTSGTTGRPKGVLSTHEN--VQAVTTGlveKW--EWKKEDVILHVLPLHHVHG 261
Cdd:COG0365 175 EFEPEPtdaDDPL-----------FILYTSGTTGKPKGVVHTHGGylVHAATTA---KYvlDLKPGDVFWCTADIGWATG 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 262 VINKLLCPLWVGATCVML---PEF-NAQMVWKKLlssQAPRISVFMAVPTIYAKLVEYYDkhffqpqvqDFVRAFCQENI 337
Cdd:COG0365 241 HSYIVYGPLLNGATVVLYegrPDFpDPGRLWELI---EKYGVTVFFTAPTAIRALMKAGD---------EPLKKYDLSSL 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 338 RLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMALSNPLHGVRI-PGSVGTPLPGVEVCIAtetlkdgarsytiha 416
Cdd:COG0365 309 RLLGSAGEPLNPEVWEWWYEAVGVPIVDGWGQTETGGIFISNLPGLPVkPGSMGKPVPGYDVAVV--------------- 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 417 qgDESNTQVTPGlegQEGELLVRG--PSVFREYWNRPRETEDAF--TPDGWFKTGDtAAYKD--GVYWIKGRTSvDIIKN 490
Cdd:COG0365 374 --DEDGNPVPPG---EEGELVIKGpwPGMFRGYWNDPERYRETYfgRFPGWYRTGD-GARRDedGYFWILGRSD-DVINV 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 491 GGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLS---VKDLKDWARDTMAPYTIPTELIVVEEI 567
Cdd:COG0365 447 SGHRIGTAEIESALVSHPAVAEAAVVGVPDEIRGQVVKAFVVLKPGVEPSdelAKELQAHVREELGPYAYPREIEFVDEL 526
|
....*...
gi 2452540125 568 PRNQMGKV 575
Cdd:COG0365 527 PKTRSGKI 534
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
63-584 |
1.76e-80 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 261.92 E-value: 1.76e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 63 GDKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHP 139
Cdd:cd05959 18 GDKTAFIDDAGSLTYAELEAEARRVA----GAL----RALgvkREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLT 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 140 VTELEYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLPLLRSCSDGS-------ASHPAVEEGPLTTCSSWKNRGAMII 212
Cdd:cd05959 90 PDDYAYYLEDSRARVVVVSGELAPVLAAALTKSEHTLVVLIVSGGAGPeagalllAELVAAEAEQLKPAATHADDPAFWL 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 213 YTSGTTGRPKGVLSTHENVQAV-------TTGLVEkwewkkEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEF-NA 284
Cdd:cd05959 170 YSSGSTGRPKGVVHLHADIYWTaelyarnVLGIRE------DDVCFSAAKLFFAYGLGNSLTFPLSVGATTVLMPERpTP 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 285 QMVWKKLlssQAPRISVFMAVPTIYAKLVEYYDkhffqPQVQDFVRafcqenIRLMVSGSAALPVPVLEKWKSITGHTLL 364
Cdd:cd05959 244 AAVFKRI---RRYRPTVFFGVPTLYAAMLAAPN-----LPSRDLSS------LRLCVSAGEALPAEVGERWKARFGLDIL 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 365 ERYGMTEIG-MALSNPLHGVRiPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTpglEGQEGELLVRGPSV 443
Cdd:cd05959 310 DGIGSTEMLhIFLSNRPGRVR-YGTTGKPVPGYEVELR-----------------DEDGGDVA---DGEPGELYVRGPSS 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 444 FREYWNRPRETEDAFTpDGWFKTGDTaaY---KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPD 520
Cdd:cd05959 369 ATMYWNNRDKTRDTFQ-GEWTRTGDK--YvrdDDGFYTYAGRAD-DMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVED 444
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 521 MVWGQRVSAVVQLRKGEMLSVK---DLKDWARDTMAPYTIPTELIVVEEIPRNQMGKvnkkelLQRF 584
Cdd:cd05959 445 EDGLTKPKAFVVLRPGYEDSEAleeELKEFVKDRLAPYKYPRWIVFVDELPKTATGK------IQRF 505
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
67-576 |
6.61e-78 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 254.44 E-value: 6.61e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 67 AIID-QNGEH-TYRDLLSQSLRLSQeicrALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVT 141
Cdd:cd05911 1 AQIDaDTGKElTYAQLRTLSRRLAA----GL----RKLglkKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTAD 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 142 ELEYVIQDSQSALVIAAEEYVGKITPSAEKLG-VPVLPLLRSCSDGSASHPAVEEGPLTTCSSW--------KNRGAMII 212
Cdd:cd05911 73 ELAHQLKISKPKVIFTDPDGLEKVKEAAKELGpKDKIIVLDDKPDGVLSIEDLLSPTLGEEDEDlppplkdgKDDTAAIL 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 213 YTSGTTGRPKGVLSTHENVQAVT--TGLVEKWEWKKEDVILHVLPLHHVHGvINKLLCPLWVGATCVMLPEFN-AQMVwk 289
Cdd:cd05911 153 YSSGTTGLPKGVCLSHRNLIANLsqVQTFLYGNDGSNDVILGFLPLYHIYG-LFTTLASLLNGATVIIMPKFDsELFL-- 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 290 KLLSSQapRISVFMAVPTIYAKLVEYydkhffqPQVQDFVrafcQENIRLMVSGSAALPVPVLEKW-KSITGHTLLERYG 368
Cdd:cd05911 230 DLIEKY--KITFLYLVPPIAAALAKS-------PLLDKYD----LSSLRVILSGGAPLSKELQELLaKRFPNATIKQGYG 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 369 MTEIGMALSNPLHGVRIPGSVGTPLPGVEVCIatetlkdgarsytihaQGDESNTQVTPGlegQEGELLVRGPSVFREYW 448
Cdd:cd05911 297 MTETGGILTVNPDGDDKPGSVGRLLPNVEAKI----------------VDDDGKDSLGPN---EPGEICVRGPQVMKGYY 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 449 NRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRV 527
Cdd:cd05911 358 NNPEATKETFDEDGWLHTGDIGYFdEDGYLYIVDR-KKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELP 436
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 2452540125 528 SAVVQLRKGEMLSVKDLKDWARDTMAPYtipTEL----IVVEEIPRNQMGKVN 576
Cdd:cd05911 437 RAYVVRKPGEKLTEKEVKDYVAKKVASY---KQLrggvVFVDEIPKSASGKIL 486
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
72-580 |
2.03e-71 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 235.65 E-value: 2.03e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 72 NGEHTYRDLLSQSLRlsqeICRAL-DCSSRdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDS 150
Cdd:cd05934 1 GRRWTYAELLRESAR----IAAALaALGIR--PGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHS 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 151 QSALVIAAEeyvgkitpsaeklgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTGRPKGVLSTHEN 230
Cdd:cd05934 75 GAQLVVVDP-------------------------------------------------ASILYTSGTTGPPKGVVITHAN 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 231 V------QAVTTGLVEkwewkkEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWKKLLSSQAPRISVFMA 304
Cdd:cd05934 106 LtfagyySARRFGLGE------DDVYLTVLPLFHINAQAVSVLAALSVGATLVLLPRFSASRFWSDVRRYGATVTNYLGA 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 305 VPTIYAKlveyydkhffQPQVQDfvrafcQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMALSNPLHGVR 384
Cdd:cd05934 180 MLSYLLA----------QPPSPD------DRAHRLRAAYGAPNPPELHEEFEERFGVRLLEGYGMTETIVGVIGPRDEPR 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 385 IPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTpglEGQEGELLVR---GPSVFREYWNRPRETEDAFtPD 461
Cdd:cd05934 244 RPGSIGRPAPGYEVRIV-----------------DDDGQELP---AGEPGELVIRglrGWGFFKGYYNMPEATAEAM-RN 302
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 462 GWFKTGDTAaYKD--GVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEML 539
Cdd:cd05934 303 GWFHTGDLG-YRDadGFFYFVDRKK-DMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETL 380
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 2452540125 540 SVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05934 381 DPEELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQL 421
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
59-580 |
4.63e-71 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 238.32 E-value: 4.63e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAIIDQNGEHTYRDLLSQSLRLSQEICRALDCSSRDlkeeRISFLCPNDASYVVAQWASWMSGGIAVPLYKKH 138
Cdd:PRK08314 20 ARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQQECGVRKGD----RVLLYMQNSPQFVIAYYAILRANAVVVPVNPMN 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 139 PVTELEYVIQDSQSALVIAAEEYVGKITPSAEKLG------------------VPVLPLLRScsdgSASHPAVEEGPLTT 200
Cdd:PRK08314 96 REEELAHYVTDSGARVAIVGSELAPKVAPAVGNLRlrhvivaqysdylpaepeIAVPAWLRA----EPPLQALAPGGVVA 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 201 CSSWKNRG-------------AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLL 267
Cdd:PRK08314 172 WKEALAAGlappphtagpddlAVLPYTSGTTGVPKGCMHTHRTVMANAVGSVLWSNSTPESVVLAVLPLFHVTGMVHSMN 251
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 268 CPLWVGATCVMLPEfnaqmvWKKLLSSQA---PRISVFMAVPTIyakLVEYydkhFFQPQVQDfvraFCQENIRLMVSGS 344
Cdd:PRK08314 252 APIYAGATVVLMPR------WDREAAARLierYRVTHWTNIPTM---VVDF----LASPGLAE----RDLSSLRYIGGGG 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 345 AALPVPVLEKWKSITGHTLLERYGMTE-IGMALSNPLHGVRiPGSVGTPLPGVEV-CIATETLKDgarsytihaqgdesn 422
Cdd:PRK08314 315 AAMPEAVAERLKELTGLDYVEGYGLTEtMAQTHSNPPDRPK-LQCLGIPTFGVDArVIDPETLEE--------------- 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 423 tqVTPgleGQEGELLVRGPSVFREYWNRPRETEDAF-TPDG--WFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISAL 498
Cdd:PRK08314 379 --LPP---GEVGEIVVHGPQVFKGYWNRPEATAEAFiEIDGkrFFRTGDLGRMdEEGYFFITDRLK-RMINASGFKVWPA 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 499 EVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLR---KGEMlSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKV 575
Cdd:PRK08314 453 EVENLLYKHPAIQEACVIATPDPRRGETVKAVVVLRpeaRGKT-TEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKI 531
|
....*
gi 2452540125 576 NKKEL 580
Cdd:PRK08314 532 LWRQL 536
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
59-584 |
6.78e-69 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 233.84 E-value: 6.78e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAIIDQNG----EHTYRDLLSQSLRLSqeicRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIA 131
Cdd:COG1022 21 AARFPDRVALREKEDgiwqSLTWAEFAERVRALA----AGL----LALgvkPGDRVAILSDNRPEWVIADLAILAAGAVT 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 132 VPLYKKHPVTELEYVIQDSQSALVIAA-EEYVGKITPSAEKLgvpvlPLLRS--CSDGSASHpavEEGPLTTCSSWKNRG 208
Cdd:COG1022 93 VPIYPTSSAEEVAYILNDSGAKVLFVEdQEQLDKLLEVRDEL-----PSLRHivVLDPRGLR---DDPRLLSLDELLALG 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 ---------------------AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLL 267
Cdd:COG1022 165 revadpaelearraavkpddlATIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLPLAHVFERTVSYY 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 268 CpLWVGATCV----------MLPEFNAQM------VWKKLLSS------QAPRI-----SVFMAVPTIYAKLVEY----- 315
Cdd:COG1022 245 A-LAAGATVAfaespdtlaeDLREVKPTFmlavprVWEKVYAGiqakaeEAGGLkrklfRWALAVGRRYARARLAgksps 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 316 ---------YDKHFFQPqvqdfVRAFCQENIRLMVSGSAALPVPVLEKWKSItGHTLLERYGMTEI-GMALSNPLHGVRI 385
Cdd:COG1022 324 lllrlkhalADKLVFSK-----LREALGGRLRFAVSGGAALGPELARFFRAL-GIPVLEGYGLTETsPVITVNRPGDNRI 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 386 pGSVGTPLPGVEVCIAtetlkdgarsytihaqgdesntqvtpglegQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFK 465
Cdd:COG1022 398 -GTVGPPLPGVEVKIA------------------------------EDGEILVRGPNVMKGYYKNPEATAEAFDADGWLH 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 466 TGDtAAY--KDGVYWIKGRTSvDIIKN-GGFKISALEVERHLLAHPHITDVAVIGppDmvwgQR--VSAVVQLRKgEMls 540
Cdd:COG1022 447 TGD-IGEldEDGFLRITGRKK-DLIVTsGGKNVAPQPIENALKASPLIEQAVVVG--D----GRpfLAALIVPDF-EA-- 515
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|..
gi 2452540125 541 vkdLKDWARDTMAPYTIPTELI----VVEEIpRNQMGKVNKK----ELLQRF 584
Cdd:COG1022 516 ---LGEWAEENGLPYTSYAELAqdpeVRALI-QEEVDRANAGlsraEQIKRF 563
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
76-580 |
2.22e-67 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 225.34 E-value: 2.22e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSQEIcRALDCSSRDlkeeRISFLCPNDASYVVAQWASWMSGGIAVPL---YKKHpvtELEYVIQDSQS 152
Cdd:cd05903 3 TYSELDTRADRLAAGL-AALGVGPGD----VVAFQLPNWWEFAVLYLACLRIGAVTNPIlpfFREH---ELAFILRRAKA 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 153 ALVIAAEEYvgkitpsaeklgvpvlpllrscsdGSASHPAVEEGPlttcsswknrgAMIIYTSGTTGRPKGVLSTHENVQ 232
Cdd:cd05903 75 KVFVVPERF------------------------RQFDPAAMPDAV-----------ALLLFTSGTTGEPKGVMHSHNTLS 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 233 AVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVwKKLLSSQapRISVFMAVPTIYAKL 312
Cdd:cd05903 120 ASIRQYAERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIWDPDKA-LALMREH--GVTFMMGATPFLTDL 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 313 VEYYDkhfFQPqvqdfvRAFCQenIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMALSNPLHG--VRIPGSVG 390
Cdd:cd05903 197 LNAVE---EAG------EPLSR--LRTFVCGGATVPRSLARRAAELLGAKVCSAYGSTECPGAVTSITPApeDRRLYTDG 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 391 TPLPGVEVCIatetlkdgarsytihaqGDESNTQVTPgleGQEGELLVRGPSVFREYWNRPRETEDAFtPDGWFKTGDTA 470
Cdd:cd05903 266 RPLPGVEIKV-----------------VDDTGATLAP---GVEGELLSRGPSVFLGYLDRPDLTADAA-PEGWFRTGDLA 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 471 AYKDGVYW-IKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDW-A 548
Cdd:cd05903 325 RLDEDGYLrITGRSK-DIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGALLTFDELVAYlD 403
|
490 500 510
....*....|....*....|....*....|..
gi 2452540125 549 RDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05903 404 RQGVAKQYWPERLVHVDDLPRTPSGKVQKFRL 435
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
64-580 |
2.44e-67 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 227.05 E-value: 2.44e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEICRALDCSsrdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:PRK06839 17 DRIAIITEEEEMTYKQLHEYVSKVAAYLIYELNVK----KGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENEL 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAAEEYVGKITPSAEKLGV-PVLPL--LRSCSDGSASH--PAVEEGPLTTCsswknrgamiiYTSGTT 218
Cdd:PRK06839 93 IFQLKDSGTTVLFVEKTFQNMALSMQKVSYVqRVISItsLKEIEDRKIDNfvEKNESASFIIC-----------YTSGTT 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 219 GRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGvINKLLCPLWVGATCVMLP-EFNAQmvwKKLLSSQAP 297
Cdd:PRK06839 162 GKPKGAVLTQENMFWNALNNTFAIDLTMHDRSIVLLPLFHIGG-IGLFAFPTLFAGGVIIVPrKFEPT---KALSMIEKH 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 298 RISVFMAVPTIYAKLVEYYDkhFFQPQVQdfvrafcqeNIRLMVSGSAALPVPVLEKWKSiTGHTLLERYGMTE----IG 373
Cdd:PRK06839 238 KVTVVMGVPTIHQALINCSK--FETTNLQ---------SVRWFYNGGAPCPEELMREFID-RGFLFGQGFGMTEtsptVF 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 374 MALSNPLHgvRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFREYWNRPRE 453
Cdd:PRK06839 306 MLSEEDAR--RKVGSIGKPVLFCDYELI-----------------DENKNKVEVG---EVGELLIRGPNVMKEYWNRPDA 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 454 TEDAFTpDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQ 532
Cdd:PRK06839 364 TEETIQ-DGWLCTGDLARVdEDGFVYIVGRKK-EMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFIV 441
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 2452540125 533 LRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK06839 442 KKSSSVLIEKDVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQL 489
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
62-584 |
2.90e-67 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 227.51 E-value: 2.90e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 62 FGDKIAIIDQNGEHTYRDLLsqslRLSQEICRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVP----L 134
Cdd:PRK08316 24 YPDKTALVFGDRSWTYAELD----AAVNRVAAAL----LDLglkKGDRVAALGHNSDAYALLWLACARAGAVHVPvnfmL 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 135 YKKhpvtELEYVIQDSQSALVIAAEEYVGKITPSAEKLgvPVLPLLRSCSDGSASHPaveeGPLTTCSSWKNRG------ 208
Cdd:PRK08316 96 TGE----ELAYILDHSGARAFLVDPALAPTAEAALALL--PVDTLILSLVLGGREAP----GGWLDFADWAEAGsvaepd 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 --------AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLP 280
Cdd:PRK08316 166 veladddlAQILYTSGTESLPKGAMLTHRALIAEYVSCIVAGDMSADDIPLHALPLYHCAQLDVFLGPYLYVGATNVILD 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 281 EFNAQMVWKKLlssQAPRISVFMAVPTIYAKLVEYYDkhFFQPQVQDFVRAFcqenirlmvSGSAALPVPVLEKwksitg 360
Cdd:PRK08316 246 APDPELILRTI---EAERITSFFAPPTVWISLLRHPD--FDTRDLSSLRKGY---------YGASIMPVEVLKE------ 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 361 htLLER---------YGMTEIGmalsnPLHGV-------RIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQ 424
Cdd:PRK08316 306 --LRERlpglrfyncYGQTEIA-----PLATVlgpeehlRRPGSAGRPVLNVETRVV-----------------DDDGND 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 425 VTPGlegQEGELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERH 503
Cdd:PRK08316 362 VAPG---EVGEIVHRSPQLMLGYWDDPEKTAEAFR-GGWFHSGDLGVMdEEGYITVVDRKK-DMIKTGGENVASREVEEA 436
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 504 LLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQR 583
Cdd:PRK08316 437 LYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATVTEDELIAHCRARLAGFKVPKRVIFVDELPRNPSGKILKRELRER 516
|
.
gi 2452540125 584 F 584
Cdd:PRK08316 517 Y 517
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
74-580 |
5.53e-66 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 221.58 E-value: 5.53e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 74 EHTYRDLLSQSLRLSQEICRaldCSSRdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSA 153
Cdd:cd05935 1 SLTYLELLEVVKKLASFLSN---KGVR--KGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAK 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 154 LVIaaeeyvgkITPSAEKLgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTGRPKGVLSTHENVQA 233
Cdd:cd05935 76 VAV--------VGSELDDL------------------------------------ALIPYTSGTTGLPKGCMHTHFSAAA 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 234 VTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWKKLlssQAPRISVFMAVPTIYAKLV 313
Cdd:cd05935 112 NALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMARWDRETALELI---EKYKVTFWTNIPTMLVDLL 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 314 EyydkhffQPQVQDfvraFCQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTE-IGMALSNPLHGVRIPgSVGTP 392
Cdd:cd05935 189 A-------TPEFKT----RDLSSLKVLTGGGAPMPPAVAEKLLKLTGLRFVEGYGLTEtMSQTHTNPPLRPKLQ-CLGIP 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 393 LPGVEvciatetlkdgARSYTIhaqgdESNTQVTPgleGQEGELLVRGPSVFREYWNRPRETEDAFTPDG---WFKTGDT 469
Cdd:cd05935 257 *FGVD-----------ARVIDI-----ETGRELPP---NEVGEIVVRGPQIFKGYWNRPEETEESFIEIKgrrFFRTGDL 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 470 aAYKD--GVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSV--KDLK 545
Cdd:cd05935 318 -GYMDeeGYFFFVDRVK-RMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYRGKVteEDII 395
|
490 500 510
....*....|....*....|....*....|....*
gi 2452540125 546 DWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05935 396 EWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
55-580 |
7.88e-66 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 223.27 E-value: 7.88e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 55 VFTKALAFGDKIAIIDQ-NGEH-TYRDLLSQSLRLSqeicRALDC---SSRDLkeerISFLCPNDASYVVAQWASWMSGG 129
Cdd:cd05904 11 SFLFASAHPSRPALIDAaTGRAlTYAELERRVRRLA----AGLAKrggRKGDV----VLLLSPNSIEFPVAFLAVLSLGA 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 130 I---AVPLYKkhpVTELEYVIQDSQSALVIAAEEYVGKITPsaekLGVPVLpLLRSCSDGSASH--PAVEEGPLTTCSSW 204
Cdd:cd05904 83 VvttANPLST---PAEIAKQVKDSGAKLAFTTAELAEKLAS----LALPVV-LLDSAEFDSLSFsdLLFEADEAEPPVVV 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 205 KNRG--AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKW--EWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLP 280
Cdd:cd05904 155 IKQDdvAALLYSSGTTGRSKGVMLTHRNLIAMVAQFVAGEgsNSDSEDVFLCVLPMFHIYGLSSFALGLLRLGATVVVMP 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 281 EFNAQmvwkKLLSS-QAPRISVFMAVPTIYAKLVEyydkhffQPQVQDFVRAfcqeNIRLMVSGSAALPVPVLEKWKSIT 359
Cdd:cd05904 235 RFDLE----ELLAAiERYKVTHLPVVPPIVLALVK-------SPIVDKYDLS----SLRQIMSGAAPLGKELIEAFRAKF 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 360 GHT-LLERYGMTE---IGMALSNPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPglEGQEGE 435
Cdd:cd05904 300 PNVdLGQGYGMTEstgVVAMCFAPEKDRAKYGSVGRLVPNVEAKIV-----------------DPETGESLP--PNQTGE 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 436 LLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTaAY--KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDV 513
Cdd:cd05904 361 LWIRGPSIMKGYLNNPEATAATIDKEGWLHTGDL-CYidEDGYLFIVDRLK-ELIKYKGFQVAPAELEALLLSHPEILDA 438
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 514 AVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05904 439 AVIPYPDEEAGEVPMAFVVRKPGSSLTEDEIMDFVAKQVAPYKKVRKVAFVDAIPKSPSGKILRKEL 505
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
64-583 |
4.86e-65 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 220.60 E-value: 4.86e-65
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSlrlsQEICRALDCSSRDlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:PRK03640 17 DRTAIEFEEKKVTFMELHEAV----VSVAGKLAALGVK-KGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREEL 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAAEEYVGKITPSAEklgVPVLPLlrscsdgsashPAVEEGPLTTCSSWK-NRGAMIIYTSGTTGRPK 222
Cdd:PRK03640 92 LWQLDDAEVKCLITDDDFEAKLIPGIS---VKFAEL-----------MNGPKEEAEIQEEFDlDEVATIMYTSGTTGKPK 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 223 GVLSTHEN--VQAVTT----GLVEKWEWkkedviLHVLPLHHVHGvINKLLCPLWVGATCVMLPEFNAQMVWKKLLSSQA 296
Cdd:PRK03640 158 GVIQTYGNhwWSAVGSalnlGLTEDDCW------LAAVPIFHISG-LSILMRSVIYGMRVVLVEKFDAEKINKLLQTGGV 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 297 PRISVfmaVPTIYAKLVEYYDKHffqpqvqdfvraFCQENIRLMVSGSAALPVPVLE--KWKSItghTLLERYGMTEIG- 373
Cdd:PRK03640 231 TIISV---VSTMLQRLLERLGEG------------TYPSSFRCMLLGGGPAPKPLLEqcKEKGI---PVYQSYGMTETAs 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 374 --MALSnPLHGVRIPGSVGTPLPGVEVCIAtetlKDGarsytihaqgdesntqvTPGLEGQEGELLVRGPSVFREYWNRP 451
Cdd:PRK03640 293 qiVTLS-PEDALTKLGSAGKPLFPCELKIE----KDG-----------------VVVPPFEEGEIVVKGPNVTKGYLNRE 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 452 RETEDAFTpDGWFKTGDTaAYKD--GVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSA 529
Cdd:PRK03640 351 DATRETFQ-DGWFKTGDI-GYLDeeGFLYVLDRRS-DLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPVA 427
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 2452540125 530 VVqlRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQR 583
Cdd:PRK03640 428 FV--VKSGEVTEEELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQL 479
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
103-582 |
1.36e-64 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 221.45 E-value: 1.36e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 103 KEERISFLCPNDASYVVAQWASWMSGGIAV---PLYKKHpvtELEYVIQDSQSALVIAAEEYVGKIT------------- 166
Cdd:PRK06710 73 KGDRVAIMLPNCPQAVIGYYGTLLAGGIVVqtnPLYTER---ELEYQLHDSGAKVILCLDLVFPRVTnvqsatkiehviv 149
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 167 -------PSAEKLGVPVLPLLRS-----CSDGSASH------PAVEEGPLTTCSSwKNRGAMIIYTSGTTGRPKGVLSTH 228
Cdd:PRK06710 150 triadflPFPKNLLYPFVQKKQSnlvvkVSESETIHlwnsveKEVNTGVEVPCDP-ENDLALLQYTGGTTGFPKGVMLTH 228
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 229 ENVQAVTTGLVEkWEW---KKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWKKLlssQAPRISVFMAV 305
Cdd:PRK06710 229 KNLVSNTLMGVQ-WLYnckEGEEVVLGVLPFFHVYGMTAVMNLSIMQGYKMVLIPKFDMKMVFEAI---KKHKVTLFPGA 304
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 306 PTIYAKLVEyydkhffqpqvQDFVRAFCQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIG-MALSNPLHGVR 384
Cdd:PRK06710 305 PTIYIALLN-----------SPLLKEYDISSIRACISGSAPLPVEVQEKFETVTGGKLVEGYGLTESSpVTHSNFLWEKR 373
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 385 IPGSVGTPLPGVEVCIATEtlkdgarsytihaqgdESNTQVTPGlegQEGELLVRGPSVFREYWNRPRETEdAFTPDGWF 464
Cdd:PRK06710 374 VPGSIGVPWPDTEAMIMSL----------------ETGEALPPG---EIGEIVVKGPQIMKGYWNKPEETA-AVLQDGWL 433
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 465 KTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKD 543
Cdd:PRK06710 434 HTGDVGYMdEDGFFYVKDRKK-DMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVVLKEGTECSEEE 512
|
490 500 510
....*....|....*....|....*....|....*....
gi 2452540125 544 LKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQ 582
Cdd:PRK06710 513 LNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVLIE 551
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
76-578 |
1.41e-64 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 218.23 E-value: 1.41e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSQEIcRALDCSsrdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALV 155
Cdd:cd05907 7 TWAEFAEEVRALAKGL-IALGVE----PGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKAL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 156 IAaeeyvgkitpsaeklgvpvlpllrscsdGSASHPAVeegplttcsswknrgamIIYTSGTTGRPKGVLSTHENVQAVT 235
Cdd:cd05907 82 FV----------------------------EDPDDLAT-----------------IIYTSGTTGRPKGVMLSHRNILSNA 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 236 TGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMvwkklLSSQAPRISVFMAVPTIYAKLvey 315
Cdd:cd05907 117 LALAERLPATEGDRHLSFLPLAHVFERRAGLYVPLLAGARIYFASSAETLL-----DDLSEVRPTVFLAVPRVWEKV--- 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 316 YDKhFFQPQVQDFVRAFCQ----ENIRLMVSGSAALPVPVLEKWKSItGHTLLERYGMTEIGMALS-NPLHGVRIpGSVG 390
Cdd:cd05907 189 YAA-IKVKAVPGLKRKLFDlavgGRLRFAASGGAPLPAELLHFFRAL-GIPVYEGYGLTETSAVVTlNPPGDNRI-GTVG 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 391 TPLPGVEVCIAtetlkdgarsytihaqgdesntqvtpglegQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTA 470
Cdd:cd05907 266 KPLPGVEVRIA------------------------------DDGEILVRGPNVMLGYYKNPEATAEALDADGWLHTGDLG 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 471 AYK-DGVYWIKGRtSVDIIKN-GGFKISALEVERHLLAHPHITDVAVIG---PpdmvwgqRVSAVVQLRKgemlsvKDLK 545
Cdd:cd05907 316 EIDeDGFLHITGR-KKDLIITsGGKNISPEPIENALKASPLISQAVVIGdgrP-------FLVALIVPDP------EALE 381
|
490 500 510
....*....|....*....|....*....|....*..
gi 2452540125 546 DWARDTMAPYTIPTELI----VVEEIpRNQMGKVNKK 578
Cdd:cd05907 382 AWAEEHGIAYTDVAELAanpaVRAEI-EAAVEAANAR 417
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
64-580 |
2.97e-61 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 209.31 E-value: 2.97e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEIcRALDCSSrdlkEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:cd05930 2 DAVAVVDGDQSLTYAELDARANRLARYL-RERGVGP----GDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERL 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAAeeyvgkitpsaeklgvpvlpllrscsdgsASHPAveegplttcsswknrgaMIIYTSGTTGRPKG 223
Cdd:cd05930 77 AYILEDSGAKLVLTD-----------------------------PDDLA-----------------YVIYTSGSTGKPKG 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 224 VLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINkLLCPLWVGATCVMLPEFNAQMVWK--KLLSSQapRISV 301
Cdd:cd05930 111 VMVEHRGLVNLLLWMQEAYPLTPGDRVLQFTSFSFDVSVWE-IFGALLAGATLVVLPEEVRKDPEAlaDLLAEE--GITV 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 302 FMAVPTIYAKLVEYydkhffqPQVQDFvrafcqENIRLMVSGSAALPVPVLEKWKSI-TGHTLLERYGMTEIGMA----- 375
Cdd:cd05930 188 LHLTPSLLRLLLQE-------LELAAL------PSLRLVLVGGEALPPDLVRRWRELlPGARLVNLYGPTEATVDatyyr 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 376 -LSNPLHGVRIPgsVGTPLPGVEVCIatetLkdgarsytihaqgDESNTQVTPGLEGqegELLVRGPSVFREYWNRPRET 454
Cdd:cd05930 255 vPPDDEEDGRVP--IGRPIPNTRVYV----L-------------DENLRPVPPGVPG---ELYIGGAGLARGYLNRPELT 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 455 EDAFTPDGWF------KTGDTAAYK-DG--VYwiKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQ 525
Cdd:cd05930 313 AERFVPNPFGpgermyRTGDLVRWLpDGnlEF--LGRID-DQVKIRGYRIELGEIEAALLAHPGVREAAVVAREDGDGEK 389
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 2452540125 526 RVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05930 390 RLVAYVVPDEGGELDEEELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRKAL 444
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
65-580 |
1.27e-59 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 204.62 E-value: 1.27e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 65 KIAIIDQNGEHTYRDLLSQSLRLSqEICRALDCSsrdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELE 144
Cdd:cd05919 1 KTAFYAADRSVTYGQLHDGANRLG-SALRNLGVS----SGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYA 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 145 YVIQDSQSALVIAAEEYVgkitpsaeklgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTGRPKGV 224
Cdd:cd05919 76 YIARDCEARLVVTSADDI----------------------------------------------AYLLYSSGTTGPPKGV 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 225 LSTHENvqavTTGLVEKWE-----WKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEF-NAQMVwkkLLSSQAPR 298
Cdd:cd05919 110 MHAHRD----PLLFADAMArealgLTPGDRVFSSAKMFFGYGLGNSLWFPLAVGASAVLNPGWpTAERV---LATLARFR 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 299 ISVFMAVPTIYAKLVeyydkhffqPQVQDFVRAFcqENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIG-MALS 377
Cdd:cd05919 183 PTVLYGVPTFYANLL---------DSCAGSPDAL--RSLRLCVSAGEALPRGLGERWMEHFGGPILDGIGATEVGhIFLS 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 378 NPLHGVRiPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFREYWNRPRETEDA 457
Cdd:cd05919 252 NRPGAWR-LGSTGRPVPGYEIRLV-----------------DEEGHTIPPG---EEGDLLVRGPSAAVGYWNNPEKSRAT 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 458 FTpDGWFKTGDTAAY-KDGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKG 536
Cdd:cd05919 311 FN-GGWYRTGDKFCRdADGWYTHAGR-ADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSP 388
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 2452540125 537 EMLSVK---DLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05919 389 AAPQESlarDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKL 435
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
59-580 |
1.97e-59 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 206.46 E-value: 1.97e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAII--DQNG---EHTYRdllsqslRLSQEICRA----LDCSSRdlKEERISFLCPNDASYVVAQWASWMSGG 129
Cdd:PRK08008 17 ADVYGHKTALIfeSSGGvvrRYSYL-------ELNEEINRTanlfYSLGIR--KGDKVALHLDNCPEFIFCWFGLAKIGA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 130 IAVPLYKKHPVTELEYVIQDSQSALVIAAEEYVGKITPSAEKLGVPV--LPLLRSCS---DGS-------ASHPAV--EE 195
Cdd:PRK08008 88 IMVPINARLLREESAWILQNSQASLLVTSAQFYPMYRQIQQEDATPLrhICLTRVALpadDGVssftqlkAQQPATlcYA 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 196 GPLTTcsswkNRGAMIIYTSGTTGRPKGVLSTHENVQavTTGLVEKWE--WKKEDVILHVLPLHHVHGVINKLLCPLWVG 273
Cdd:PRK08008 168 PPLST-----DDTAEILFTSGTTSRPKGVVITHYNLR--FAGYYSAWQcaLRDDDVYLTVMPAFHIDCQCTAAMAAFSAG 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 274 ATCVMLPEFNAQMVWKKLLSSQApriSVFMAVPTIYAKLVeyydkhfFQPQVQDfVRAFCqenIRLMVsgsAALPVPVLE 353
Cdd:PRK08008 241 ATFVLLEKYSARAFWGQVCKYRA---TITECIPMMIRTLM-------VQPPSAN-DRQHC---LREVM---FYLNLSDQE 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 354 KWKSIT--GHTLLERYGMTE--IGMALSNPLHGVRIPgSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGl 429
Cdd:PRK08008 304 KDAFEErfGVRLLTSYGMTEtiVGIIGDRPGDKRRWP-SIGRPGFCYEAEIR-----------------DDHNRPLPAG- 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 430 egQEGELLVRG---PSVFREYWNRPRETEDAFTPDGWFKTGDTAaYKD--GVYWIKGRtSVDIIKNGGFKISALEVERHL 504
Cdd:PRK08008 365 --EIGEICIKGvpgKTIFKEYYLDPKATAKVLEADGWLHTGDTG-YVDeeGFFYFVDR-RCNMIKRGGENVSCVELENII 440
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 505 LAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK08008 441 ATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEEEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNL 516
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
64-585 |
3.19e-59 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 205.99 E-value: 3.19e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQeicrALdcSSRDL-KEERISFLCPNDASYVVAQWASWMSGGIAVPLykkHPVTE 142
Cdd:PRK06188 27 DRPALVLGDTRLTYGQLADRISRYIQ----AF--EALGLgTGDAVALLSLNRPEVLMAIGAAQLAGLRRTAL---HPLGS 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 143 LE---YVIQDSQ-SALVIAAEEYVGKITPSAEK---------LG-VPVLPLLRSCSDGSASHPAVEEGpLTTCSSWknrg 208
Cdd:PRK06188 98 LDdhaYVLEDAGiSTLIVDPAPFVERALALLARvpslkhvltLGpVPDGVDLLAAAAKFGPAPLVAAA-LPPDIAG---- 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 amIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVinKLLCPLWVGATCVMLPEFNAQMVw 288
Cdd:PRK06188 173 --LAYTGGTTGKPKGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSHAGGA--FFLPTLLRGGTVIVLAKFDPAEV- 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 kkLLSSQAPRISVFMAVPT-IYAkLVEYYDKHffqpqVQDFvrafcqENIRLMVSGSAAL-PVPVLEKWKSItGHTLLER 366
Cdd:PRK06188 248 --LRAIEEQRITATFLVPTmIYA-LLDHPDLR-----TRDL------SSLETVYYGASPMsPVRLAEAIERF-GPIFAQY 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 367 YGMTEIGMA---LSNPLHGVRIP---GSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRG 440
Cdd:PRK06188 313 YGQTEAPMVityLRKRDHDPDDPkrlTSCGRPTPGLRVALL-----------------DEDGREVAQG---EVGEICVRG 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 441 PSVFREYWNRPRETEDAFTpDGWFKTGDTA-AYKDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPP 519
Cdd:PRK06188 373 PLVMDGYWNRPEETAEAFR-DGWLHTGDVArEDEDGFYYIVDRKK-DMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVP 450
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 520 DMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQRFY 585
Cdd:PRK06188 451 DEKWGEAVTAVVVLRPGAAVDAAELQAHVKERKGSVHAPKQVDFVDSLPLTALGKPDKKALRARYW 516
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
63-584 |
6.66e-59 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 204.69 E-value: 6.66e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 63 GDKIAIIDQNGEHTYRDLLSQSLRLSQEIcRALDCSsrdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTE 142
Cdd:TIGR02262 19 GGKTAFIDDISSLSYGELEAQVRRLAAAL-RRLGVK----REERVLLLMLDGVDFPIAFLGAIRAGIVPVALNTLLTADD 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 143 LEYVIQDSQSALVIAAEEYVGKITPSAEKlgVPVLPLLRSCSDGSASHP------AVEEGPLTTCSSWKNRGAMIIYTSG 216
Cdd:TIGR02262 94 YAYMLEDSRARVVFVSGALLPVIKAALGK--SPHLEHRVVVGRPEAGEVqlaellATESEQFKPAATQADDPAFWLYSSG 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 217 TTGRPKGVLSTHENVQAV-------TTGLVEkwewkkEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEF-NAQMVW 288
Cdd:TIGR02262 172 STGMPKGVVHTHSNPYWTaelyarnTLGIRE------DDVCFSAAKLFFAYGLGNALTFPMSVGATTVLMGERpTPDAVF 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 KKLLSSQApriSVFMAVPTIYAKLVEYYDkhffqpqvqdfVRAFCQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYG 368
Cdd:TIGR02262 246 DRLRRHQP---TIFYGVPTLYAAMLADPN-----------LPSEDQVRLRLCTSAGEALPAEVGQRWQARFGVDIVDGIG 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 369 MTEIG-MALSNPLHGVRIpGSVGTPLPGvevciatetlkdgarsYTIHAQGDESNtqvtPGLEGQEGELLVRGPSVFREY 447
Cdd:TIGR02262 312 STEMLhIFLSNLPGDVRY-GTSGKPVPG----------------YRLRLVGDGGQ----DVADGEPGELLISGPSSATMY 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 448 WNRPRETEDAFTpDGWFKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQR 526
Cdd:TIGR02262 371 WNNRAKSRDTFQ-GEWTRSGDKYVRNdDGSYTYAGRTD-DMLKVSGIYVSPFEIESALIQHPAVLEAAVVGVADEDGLIK 448
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 2452540125 527 VSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKvnkkelLQRF 584
Cdd:TIGR02262 449 PKAFVVLRPGQTALETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGK------IQRF 500
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
64-582 |
8.05e-59 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 205.29 E-value: 8.05e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEH------TYRDLLsqslRLSQEICRALdcssRDLKEER---ISFLCPNDASYVVAQWASWMSGGIAVPL 134
Cdd:PRK13295 39 DKTAVTAVRLGTgaprrfTYRELA----ALVDRVAVGL----ARLGVGRgdvVSCQLPNWWEFTVLYLACSRIGAVLNPL 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 135 ---YKKHpvtELEYVIQDSQSALVIAAEEYVGKITPS-AEKLGvPVLPLLRSC----SDGSAS------HPAVEEGPLTT 200
Cdd:PRK13295 111 mpiFRER---ELSFMLKHAESKVLVVPKTFRGFDHAAmARRLR-PELPALRHVvvvgGDGADSfealliTPAWEQEPDAP 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 201 CSSWKNRG-----AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGAT 275
Cdd:PRK13295 187 AILARLRPgpddvTQLIYTSGTTGEPKGVMHTANTLMANIVPYAERLGLGADDVILMASPMAHQTGFMYGLMMPVMLGAT 266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 276 CVMlpefnaQMVWKKLLSS---QAPRISVFMAVPTIYAKLVEYYDKhffQPQVQDFVRAFCqenirlmvSGSAALPVPVL 352
Cdd:PRK13295 267 AVL------QDIWDPARAAeliRTEGVTFTMASTPFLTDLTRAVKE---SGRPVSSLRTFL--------CAGAPIPGALV 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 353 EKWKSITGHTLLERYGMTEIGMA----LSNPLHgvRIPGSVGTPLPGVEVCIatetlkdgarsytIHAQGdesntqvTPG 428
Cdd:PRK13295 330 ERARAALGAKIVSAWGMTENGAVtltkLDDPDE--RASTTDGCPLPGVEVRV-------------VDADG-------APL 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 429 LEGQEGELLVRGPSVFREYWNRPRETEDAFtpDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAH 507
Cdd:PRK13295 388 PAGQIGRLQVRGCSNFGGYLKRPQLNGTDA--DGWFDTGDLARIdADGYIRISGRSK-DVIIRGGENIPVVEIEALLYRH 464
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2452540125 508 PHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDT-MAPYTIPTELIVVEEIPRNQMGKVNK---KELLQ 582
Cdd:PRK13295 465 PAIAQVAIVAYPDERLGERACAFVVPRPGQSLDFEEMVEFLKAQkVAKQYIPERLVVRDALPRTPSGKIQKfrlREMLR 543
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
74-580 |
2.11e-58 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 203.63 E-value: 2.11e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 74 EHTYRDLLSQSLRLSQeicrALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDS 150
Cdd:cd12119 25 RYTYAEVAERARRLAN----AL----RRLgvkPGDRVATLAWNTHRHLELYYAVPGMGAVLHTINPRLFPEQIAYIINHA 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 151 QSALVIAAEEYVGKITPSAEKLG-VPVLPLLRSCSDGSASHP--------AVEEGPLTTcsSWK----NRGAMIIYTSGT 217
Cdd:cd12119 97 EDRVVFVDRDFLPLLEAIAPRLPtVEHVVVMTDDAAMPEPAGvgvlayeeLLAAESPEY--DWPdfdeNTAAAICYTSGT 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 218 TGRPKGVLSTHENV--------QAVTTGLVEKwewkkeDVILHVLPLHHVH--GVinkllcP---LWVGATCV-----ML 279
Cdd:cd12119 175 TGNPKGVVYSHRSLvlhamaalLTDGLGLSES------DVVLPVVPMFHVNawGL------PyaaAMVGAKLVlpgpyLD 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 280 PEFNAQmvwkkLLSSQAPRISVfmAVPTIYAKLVEYYDKHffqPQVQDFVRAfcqenirlMVSGSAALPVPVLEKWKSIt 359
Cdd:cd12119 243 PASLAE-----LIEREGVTFAA--GVPTVWQGLLDHLEAN---GRDLSSLRR--------VVIGGSAVPRSLIEAFEER- 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 360 GHTLLERYGMTE---IG-MALSNPLH-------GVRIPGSVGTPLPGVEVCIATEtlkdgarsytihaqgdesNTQVTPG 428
Cdd:cd12119 304 GVRVIHAWGMTEtspLGtVARPPSEHsnlsedeQLALRAKQGRPVPGVELRIVDD------------------DGRELPW 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 429 LEGQEGELLVRGPSVFREYWNRPRETEdAFTPDGWFKTGDTAA-YKDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAH 507
Cdd:cd12119 366 DGKAVGELQVRGPWVTKSYYKNDEESE-ALTEDGWLRTGDVATiDEDGYLTITDRSK-DVIKSGGEWISSVELENAIMAH 443
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2452540125 508 PHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd12119 444 PAVAEAAVIGVPHPKWGERPLAVVVLKEGATVTAEELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKIDKKAL 516
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
59-584 |
4.42e-58 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 203.07 E-value: 4.42e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAIIDQNGEHTYRDLLSQSLRLsqeicrALDCSSRDL-KEERISFLCPNDASYVVAQWASWMSGgiAVPLYK- 136
Cdd:COG1021 35 AERHPDRIAVVDGERRLSYAELDRRADRL------AAGLLALGLrPGDRVVVQLPNVAEFVIVFFALFRAG--AIPVFAl 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 137 -KHPVTELEYVIQDSQSALVIAAEEYvgkitpsaekLGVPVLPLLRscsDGSASHPAVEE-------GPLTTCSSWKNRG 208
Cdd:COG1021 107 pAHRRAEISHFAEQSEAVAYIIPDRH----------RGFDYRALAR---ELQAEVPSLRHvlvvgdaGEFTSLDALLAAP 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIY-------------TSGTTGRPKGVLSTHE----NVQAvttgLVEKWEWKKEDVILHVLPLHH--------VHGVi 263
Cdd:COG1021 174 ADLSEprpdpddvaffqlSGGTTGLPKLIPRTHDdylySVRA----SAEICGLDADTVYLAALPAAHnfplsspgVLGV- 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 264 nkllcpLWVGATCVMLPEFNAQMVWKkLLSSQapRISVFMAVPTIYAKLVEYYDKHFFQPqvqdfvrafcqENIRLMVSG 343
Cdd:COG1021 249 ------LYAGGTVVLAPDPSPDTAFP-LIERE--RVTVTALVPPLALLWLDAAERSRYDL-----------SSLRVLQVG 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 344 SAALPVPVLEKWKSITGHTLLERYGMTEiGM----ALSNPLHgvRIPGSVGTPL-PGVEVCIAtetlkdgarsytihaqg 418
Cdd:COG1021 309 GAKLSPELARRVRPALGCTLQQVFGMAE-GLvnytRLDDPEE--VILTTQGRPIsPDDEVRIV----------------- 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 419 DESNTQVTPGlegQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRtSVDIIKNGGFKISA 497
Cdd:COG1021 369 DEDGNPVPPG---EVGELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVRRtPDGYLVVEGR-AKDQINRGGEKIAA 444
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 498 LEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRkGEMLSVKDLKDWARDT-MAPYTIPTELIVVEEIPRNQMGKVN 576
Cdd:COG1021 445 EEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPR-GEPLTLAELRRFLRERgLAAFKLPDRLEFVDALPLTAVGKID 523
|
....*...
gi 2452540125 577 KKELLQRF 584
Cdd:COG1021 524 KKALRAAL 531
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
76-580 |
1.83e-57 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 198.72 E-value: 1.83e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLsqslRLSQEICRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQS 152
Cdd:cd05972 2 SFRELK----RESAKAANVL----AKLglrKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGA 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 153 ALVIAAEEYVgkitpsaeklgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTGRPKGVLSTHENVQ 232
Cdd:cd05972 74 KAIVTDAEDP----------------------------------------------ALIYFTSGTTGLPKGVLHTHSYPL 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 233 AVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVM--LPEFNAQmVWKKLLSSQapRISVFMAVPTIYA 310
Cdd:cd05972 108 GHIPTAAYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVyeGPRFDAE-RILELLERY--GVTSFCGPPTAYR 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 311 KLVEYYDKHFfqpqvqDFVRafcqenIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMALSNpLHGVRI-PGSV 389
Cdd:cd05972 185 MLIKQDLSSY------KFSH------LRLVVSAGEPLNPEVIEWWRAATGLPIRDGYGQTETGLTVGN-FPDMPVkPGSM 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 390 GTPLPGVEVCIatetlkdgarsytIHAQGDEsntqVTPGlegQEGELLVR--GPSVFREYWNRPRETEDAFTpDGWFKTG 467
Cdd:cd05972 252 GRPTPGYDVAI-------------IDDDGRE----LPPG---EEGDIAIKlpPPGLFLGYVGDPEKTEASIR-GDYYLTG 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 468 DTAaYKD--GVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLS---VK 542
Cdd:cd05972 311 DRA-YRDedGYFWFVGRAD-DIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSeelAE 388
|
490 500 510
....*....|....*....|....*....|....*...
gi 2452540125 543 DLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05972 389 ELQGHVKKVLAPYKYPREIEFVEELPKTISGKIRRVEL 426
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
57-583 |
5.59e-57 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 200.36 E-value: 5.59e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 57 TKALAFGDKIAIIDQNG-EHTYRDLLSQSLRLSQEIcraLDCSSRDLkeERISFLCPNDASYVVAQWASWMSGGIAVPLY 135
Cdd:PRK06087 31 QTARAMPDKIAVVDNHGaSYTYSALDHAASRLANWL---LAKGIEPG--DRVAFQLPGWCEFTIIYLACLKVGAVSVPLL 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 136 KKHPVTELEYVIQDSQSALVIAAEeYVGKITPsaEKLGVPV---LPLLR----------SCSDGSASHPAVEEGPLTT-C 201
Cdd:PRK06087 106 PSWREAELVWVLNKCQAKMFFAPT-LFKQTRP--VDLILPLqnqLPQLQqivgvdklapATSSLSLSQIIADYEPLTTaI 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 202 SSWKNRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPE 281
Cdd:PRK06087 183 TTHGDELAAVLFTSGTEGLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDI 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 282 FNAQMVWkKLLSSQapRISVFM-AVPTIYAKLVEyydkhfFQPQVQDFvrafcqENIRLMVSGSAALPVPVLEKWKSiTG 360
Cdd:PRK06087 263 FTPDACL-ALLEQQ--RCTCMLgATPFIYDLLNL------LEKQPADL------SALRFFLCGGTTIPKKVARECQQ-RG 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 361 HTLLERYGMTE----IGMALSNPLHgvRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGLEGQEGEl 436
Cdd:PRK06087 327 IKLLSVYGSTEssphAVVNLDDPLS--RFMHTDGYAAAGVEIKVV-----------------DEARKTLPPGCEGEEAS- 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 437 lvRGPSVFREYWNRPRETEDAFTPDGWFKTGDTaAYKD--GVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVA 514
Cdd:PRK06087 387 --RGPNVFMGYLDEPELTARALDEEGWYYSGDL-CRMDeaGYIKITGRKK-DIIVRGGENISSREVEDILLQHPKIHDAC 462
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2452540125 515 VIGPPDMVWGQRVSAVVQLRKGE-MLSVKDLKDW-ARDTMAPYTIPTELIVVEEIPRNQMGKVNK----KELLQR 583
Cdd:PRK06087 463 VVAMPDERLGERSCAYVVLKAPHhSLTLEEVVAFfSRKRVAKYKYPEHIVVIDKLPRTASGKIQKfllrKDIMRR 537
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
105-580 |
1.17e-56 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 198.11 E-value: 1.17e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 105 ERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALViaaeeyVGKITPSAEKLGVPVLPLLRSCS 184
Cdd:PRK09088 48 ERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEPRLL------LGDDAVAAGRTDVEDLAAFIASA 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 185 DGsashpaveEGPLTTCSSWKNRGAMIIYTSGTTGRPKGVLSTHENVQ--AVTTGLVEKWEwkKEDVILHVLPLHHVHGV 262
Cdd:PRK09088 122 DA--------LEPADTPSIPPERVSLILFTSGTSGQPKGVMLSERNLQqtAHNFGVLGRVD--AHSSFLCDAPMFHIIGL 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 263 INKLLCPLWVGATCVMLPEFNAQMVWKKLlSSQAPRISVFMAVPTIYAKLVEyydkhffQPqvqDFVRAFCQeNIRLMVS 342
Cdd:PRK09088 192 ITSVRPVLAVGGSILVSNGFEPKRTLGRL-GDPALGITHYFCVPQMAQAFRA-------QP---GFDAAALR-HLTALFT 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 343 GSAALPVPVLEKWKSiTGHTLLERYGMTEIGMALSNPLHGVRIP---GSVGTPLPGVEVCIAtetlkdgarsytihaqgD 419
Cdd:PRK09088 260 GGAPHAAEDILGWLD-DGIPMVDGFGMSEAGTVFGMSVDCDVIRakaGAAGIPTPTVQTRVV-----------------D 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 420 ESNTQVTPGLEGqegELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTA-AYKDGVYWIKGRTSvDIIKNGGFKISAL 498
Cdd:PRK09088 322 DQGNDCPAGVPG---ELLLRGPNLSPGYWRRPQATARAFTGDGWFRTGDIArRDADGFFWVVDRKK-DMFISGGENVYPA 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 499 EVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKK 578
Cdd:PRK09088 398 EIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAPLDLERIRSHLSTRLAKYKVPKHLRLVDALPRTASGKLQKA 477
|
..
gi 2452540125 579 EL 580
Cdd:PRK09088 478 RL 479
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
103-582 |
1.24e-56 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 196.03 E-value: 1.24e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 103 KEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALviaaeeyvgkitpsaeklgvpvlpllrs 182
Cdd:cd05912 25 KGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDVKL---------------------------- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 183 csdgsashpaveegplttcsswkNRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGv 262
Cdd:cd05912 77 -----------------------DDIATIMYTSGTTGKPKGVQQTFGNHWWSAIGSALNLGLTEDDNWLCALPLFHISG- 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 263 INKLLCPLWVGATCVMLPEFNAQMVWKKLlssQAPRISVFMAVPTIYAKLVEYYDKHffqpqvqdfvrafCQENIRLMVS 342
Cdd:cd05912 133 LSILMRSVIYGMTVYLVDKFDAEQVLHLI---NSGKVTIISVVPTMLQRLLEILGEG-------------YPNNLRCILL 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 343 GSAALPVPVLEKWKSiTGHTLLERYGMTEIG--MALSNPLHGVRIPGSVGTPLPGVEVCIATETlkdgarsytihaqgde 420
Cdd:cd05912 197 GGGPAPKPLLEQCKE-KGIPVYQSYGMTETCsqIVTLSPEDALNKIGSAGKPLFPVELKIEDDG---------------- 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 421 sntqvtpGLEGQEGELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTaAYKD--GVYWIKGRTSvDIIKNGGFKISAL 498
Cdd:cd05912 260 -------QPPYEVGEILLKGPNVTKGYLNRPDATEESFE-NGWFKTGDI-GYLDeeGFLYVLDRRS-DLIISGGENIYPA 329
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 499 EVERHLLAHPHITDVAVIGPPDMVWGQR-VSAVVQLRKgemLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNK 577
Cdd:cd05912 330 EIEEVLLSHPAIKEAGVVGIPDDKWGQVpVAFVVSERP---ISEEELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLR 406
|
....*
gi 2452540125 578 KELLQ 582
Cdd:cd05912 407 HELKQ 411
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
59-580 |
2.20e-55 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 194.83 E-value: 2.20e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAIIDQNGEHTYRDLLSQSLRLSQEIcRALDCSSRDlkeeRISFLCPNDASYVVAQWASWMSGGIAVPLYKKH 138
Cdd:cd12118 14 AAVYPDRTSIVYGDRRYTWRQTYDRCRRLASAL-AALGISRGD----TVAVLAPNTPAMYELHFGVPMAGAVLNALNTRL 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 139 PVTELEYVIQDSQSALVIAAEEYVGkitpsaEKL---GVPVLPLLRscsdgsashPAVEEGPLTtcsswknrgamIIYTS 215
Cdd:cd12118 89 DAEEIAFILRHSEAKVLFVDREFEY------EDLlaeGDPDFEWIP---------PADEWDPIA-----------LNYTS 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 216 GTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGvinklLCPLW----VGATCVMLPEFNAQMVWKKL 291
Cdd:cd12118 143 GTTGRPKGVVYHHRGAYLNALANILEWEMKQHPVYLWTLPMFHCNG-----WCFPWtvaaVGGTNVCLRKVDAKAIYDLI 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 292 lssQAPRISVFMAVPTIYAKLVEYydkhffQPQVQdfvRAFcQENIRLMVSGSAAlPVPVLEKWKSItGHTLLERYGMTE 371
Cdd:cd12118 218 ---EKHKVTHFCGAPTVLNMLANA------PPSDA---RPL-PHRVHVMTAGAPP-PAAVLAKMEEL-GFDVTHVYGLTE 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 372 I-GMALSNPLH------------------GVRIPGsvgtpLPGVEVcIATETLKDGARsytihaqgdesntqvtpglEGQ 432
Cdd:cd12118 283 TyGPATVCAWKpewdelpteerarlkarqGVRYVG-----LEEVDV-LDPETMKPVPR-------------------DGK 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 433 E-GELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTAA-YKDGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHI 510
Cdd:cd12118 338 TiGEIVFRGNIVMKGYLKNPEATAEAFR-GGWFHSGDLAViHPDGYIEIKDR-SKDIIISGGENISSVEVEGVLYKHPAV 415
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 511 TDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTElIVVEEIPRNQMGKVNKKEL 580
Cdd:cd12118 416 LEAAVVARPDEKWGEVPCAFVELKEGAKVTEEEIIAFCREHLAGFMVPKT-VVFGELPKTSTGKIQKFVL 484
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
210-577 |
5.31e-55 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 189.40 E-value: 5.31e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 210 MIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGvINKLLCPLWVGATCVMLPEFNAQMVWK 289
Cdd:cd17637 4 VIIHTAAVAGRPRGAVLSHGNLIAANLQLIHAMGLTEADVYLNMLPLFHIAG-LNLALATFHAGGANVVMEKFDPAEALE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 290 KLlssQAPRISVFMAVPTIYAKLVEYYDKHFFQPqvqDFVRAfcqenirlmVSGsaaLPVP-VLEKWKSITGHTLLERYG 368
Cdd:cd17637 83 LI---EEEKVTLMGSFPPILSNLLDAAEKSGVDL---SSLRH---------VLG---LDAPeTIQRFEETTGATFWSLYG 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 369 MTEIGMALSnpLHGVR-IPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFREY 447
Cdd:cd17637 145 QTETSGLVT--LSPYReRPGSAGRPGPLVRVRIV-----------------DDNDRPVPAG---ETGEIVVRGPLVFQGY 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 448 WNRPRETEDAFTpDGWFKTGDTAAY-KDGVYWIKGRTSV-DIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQ 525
Cdd:cd17637 203 WNLPELTAYTFR-NGWHHTGDLGRFdEDGYLWYAGRKPEkELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGE 281
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|..
gi 2452540125 526 RVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNK 577
Cdd:cd17637 282 GIKAVCVLKPGATLTADELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
63-580 |
7.67e-55 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 192.93 E-value: 7.67e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 63 GDKIAIIDQNGEHTYRDLLSQSLRLSqeicraldCSSRDL---KEERISFLCPNDASYVVAQWASWMSGGI---AVPLYK 136
Cdd:cd05920 29 PDRIAVVDGDRRLTYRELDRRADRLA--------AGLRGLgirPGDRVVVQLPNVAEFVVLFFALLRLGAVpvlALPSHR 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 137 KHpvtELEYVIQDSQSALVIAAEEYVGkitpsaeklgVPVLPLLRscsDGSASHPAVeegplttcsswknrgAMIIYTSG 216
Cdd:cd05920 101 RS---ELSAFCAHAEAVAYIVPDRHAG----------FDHRALAR---ELAESIPEV---------------ALFLLSGG 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 217 TTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHvhgviN-KLLCP-----LWVGATCVMLPEFNAQMVWKK 290
Cdd:cd05920 150 TTGTPKLIPRTHNDYAYNVRASAEVCGLDQDTVYLAVLPAAH-----NfPLACPgvlgtLLAGGRVVLAPDPSPDAAFPL 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 291 LlssQAPRISVFMAVPTIYAKLVEYYDKHFFQPQvqdfvrafcqeNIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMT 370
Cdd:cd05920 225 I---EREGVTVTALVPALVSLWLDAAASRRADLS-----------SLRLLQVGGARLSPALARRVPPVLGCTLQQVFGMA 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 371 EiGM----ALSNPlhGVRIPGSVGTPL-PGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFR 445
Cdd:cd05920 291 E-GLlnytRLDDP--DEVIIHTQGRPMsPDDEIRVV-----------------DEEGNPVPPG---EEGELLTRGPYTIR 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 446 EYWNRPRETEDAFTPDGWFKTGDTAA-YKDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWG 524
Cdd:cd05920 348 GYYRAPEHNARAFTPDGFYRTGDLVRrTPDGYLVVEGRIK-DQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDELLG 426
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 525 QRVSAVVQLRkGEMLSVKDLKDWARDT-MAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05920 427 ERSCAFVVLR-DPPPSAAQLRRFLRERgLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
211-580 |
8.99e-55 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 189.03 E-value: 8.99e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 211 IIYTSGTTGRPKGVLSTHENV--QAVTTGlvEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVML-PEFNAQMV 287
Cdd:cd05917 7 IQFTSGTTGSPKGATLTHHNIvnNGYFIG--ERLGLTEQDRLCIPVPLFHCFGSVLGVLACLTHGATMVFPsPSFDPLAV 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 288 wkkLLSSQAPRISVFMAVPTIYAKLVEYYDKHFFQPQvqdfvrafcqeNIRLMVSGSAALPVPVLEKWKSITGHT-LLER 366
Cdd:cd05917 85 ---LEAIEKEKCTALHGVPTMFIAELEHPDFDKFDLS-----------SLRTGIMAGAPCPPELMKRVIEVMNMKdVTIA 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 367 YGMTE----IGMALSNPLHGVRIpGSVGTPLPGVEVCIATETlkdgarsytihaqgdesnTQVTPGLeGQEGELLVRGPS 442
Cdd:cd05917 151 YGMTEtspvSTQTRTDDSIEKRV-NTVGRIMPHTEAKIVDPE------------------GGIVPPV-GVPGELCIRGYS 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 443 VFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDM 521
Cdd:cd05917 211 VMKGYWNDPEKTAEAIDGDGWLHTGDLAVMdEDGYCRIVGRIK-DMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDE 289
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*....
gi 2452540125 522 VWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05917 290 RYGEEVCAWIRLKEGAELTEEDIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKL 348
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
214-580 |
3.91e-54 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 194.40 E-value: 3.91e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 214 TSGTTGRPKGVLSTHEN--VQAVTTGLVEKWewKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVML-------PEFNA 284
Cdd:PRK07529 221 TGGTTGMPKLAQHTHGNevANAWLGALLLGL--GPGDTVFCGLPLFHVNALLVTGLAPLARGAHVVLAtpqgyrgPGVIA 298
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 285 QMvWKKLlssQAPRISVFMAVPTIYAKLVEyydkhffQPqvqdfVRAFCQENIRLMVSGSAALPVPVLEKWKSITGHTLL 364
Cdd:PRK07529 299 NF-WKIV---ERYRINFLSGVPTVYAALLQ-------VP-----VDGHDISSLRYALCGAAPLPVEVFRRFEAATGVRIV 362
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 365 ERYGMTEiGMALS--NPLHGVRIPGSVGTPLPGVEVCIATetlKDGARSYTIHAQGDEsntqvtpglegqEGELLVRGPS 442
Cdd:PRK07529 363 EGYGLTE-ATCVSsvNPPDGERRIGSVGLRLPYQRVRVVI---LDDAGRYLRDCAVDE------------VGVLCIAGPN 426
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 443 VFREYWNrPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDM 521
Cdd:PRK07529 427 VFSGYLE-AAHNKGLWLEDGWLNTGDLGRIdADGYFWLTGRAK-DLIIRGGHNIDPAAIEEALLRHPAVALAAAVGRPDA 504
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 522 VWGQRVSAVVQLRKGEMLSVKDLKDWARDTMA-PYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK07529 505 HAGELPVAYVQLKPGASATEAELLAFARDHIAeRAAVPKHVRILDALPKTAVGKIFKPAL 564
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
64-580 |
7.79e-54 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 189.38 E-value: 7.79e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEIcraldcSSRDLKEE-RISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTE 142
Cdd:cd05945 6 DRPAVVEGGRTLTYRELKERADALAAAL------ASLGLDAGdPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAER 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 143 LEYVIQDSQSALVIAAEEYVgkitpsaeklgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTGRPK 222
Cdd:cd05945 80 IREILDAAKPALLIADGDDN----------------------------------------------AYIIFTSGSTGRPK 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 223 GVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLH---HVHGvinkLLCPLWVGATCVMLPEfnAQMVWKKLLSSQAPR- 298
Cdd:cd05945 114 GVQISHDNLVSFTNWMLSDFPLGPGDVFLNQAPFSfdlSVMD----LYPALASGATLVPVPR--DATADPKQLFRFLAEh 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 299 -ISVFMAVPTIYAKLVEyyDKHFFQ---PQVQDFVraFCQEnirlmvsgsaALPVPVLEKWKSIT-GHTLLERYGMTEIG 373
Cdd:cd05945 188 gITVWVSTPSFAAMCLL--SPTFTPeslPSLRHFL--FCGE----------VLPHKTARALQQRFpDARIYNTYGPTEAT 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 374 MALS------NPLHGV-RIPgsVGTPLPGVEVCIatetlkdgarsytIHAQGDesntqvtPGLEGQEGELLVRGPSVFRE 446
Cdd:cd05945 254 VAVTyievtpEVLDGYdRLP--IGYAKPGAKLVI-------------LDEDGR-------PVPPGEKGELVISGPSVSKG 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 447 YWNRPRETEDAFTPD---GWFKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMV 522
Cdd:cd05945 312 YLNNPEKTAAAFFPDegqRAYRTGDLVRLEaDGLLFYRGRLD-FQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGE 390
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 2452540125 523 WGQRVSAVVQLRKG-EMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05945 391 KVTELIAFVVPKPGaEAGLTKAIKAELAERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
74-584 |
1.11e-53 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 188.84 E-value: 1.11e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 74 EHTYRDLLSqslrLSQEICRALDCSSRDLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSA 153
Cdd:cd05958 10 EWTYRDLLA----LANRIANVLVGELGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDKARIT 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 154 LVIAAEeyvgKITPSAEklgvpvlpllrscsdgsashpaveegpltTCSswknrgamIIYTSGTTGRPKGVLSTHENVQA 233
Cdd:cd05958 86 VALCAH----ALTASDD-----------------------------ICI--------LAFTSGTTGAPKATMHFHRDPLA 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 234 VTTGL-VEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWKkLLSSQAPriSVFMAVPTIYAKL 312
Cdd:cd05958 125 SADRYaVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEEATPDLLLS-AIARYKP--TVLFTAPTAYRAM 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 313 VEYYDkhFFQPQVQdfvrafcqeNIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTE-IGMALSNPLHGVRiPGSVGT 391
Cdd:cd05958 202 LAHPD--AAGPDLS---------SLRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTEmFHIFISARPGDAR-PGATGK 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 392 PLPGvevciatetlkdgarsYTIHAQGDESNTqvTPglEGQEGELLVRGPSVfreYWNRPRETEDAFTPDGWFKTGDTAA 471
Cdd:cd05958 270 PVPG----------------YEAKVVDDEGNP--VP--DGTIGRLAVRGPTG---CRYLADKRQRTYVQGGWNITGDTYS 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 472 YK-DGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLS---VKDLKDW 547
Cdd:cd05958 327 RDpDGYFRHQGR-SDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGpvlARELQDH 405
|
490 500 510
....*....|....*....|....*....|....*..
gi 2452540125 548 ARDTMAPYTIPTELIVVEEIPRNQMGKvnkkelLQRF 584
Cdd:cd05958 406 AKAHIAPYKYPRAIEFVTELPRTATGK------LQRF 436
|
|
| menE |
TIGR01923 |
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ... |
76-580 |
1.12e-52 |
|
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]
Pssm-ID: 162605 [Multi-domain] Cd Length: 436 Bit Score: 186.12 E-value: 1.12e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSQeicrALDCSSRDlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALV 155
Cdd:TIGR01923 1 TWQDLDCEAAHLAK----ALKAQGIR-SGSRVALVGQNSIEMVLLLHACLLLGAEIAMLNTRLTENERTNQLEDLDVQLL 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 156 IAA----EEYVGKItpSAEKLGVPvlpllrSCSDGSASHPAVEEGPlttcsswknrgAMIIYTSGTTGRPKGVLSTHENV 231
Cdd:TIGR01923 76 LTDslleEKDFQAD--SLDRIEAA------GRYETSLSASFNMDQI-----------ATLMFTSGTTGKPKAVPHTFRNH 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 232 QAVTTGLVEKWEWKKEDVILHVLPLHHVHGViNKLLCPLWVGATCVMLPEFNAqmvwkkLLSSQA-PRISVFMAVPTIYA 310
Cdd:TIGR01923 137 YASAVGSKENLGFTEDDNWLLSLPLYHISGL-SILFRWLIEGATLRIVDKFNQ------LLEMIAnERVTHISLVPTQLN 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 311 KLVEyydkhffqpqvqdfvRAFCQENIRLMVSGSAALPVPVLEKWKSiTGHTLLERYGMTEIG---MALSNPLHGVRipG 387
Cdd:TIGR01923 210 RLLD---------------EGGHNENLRKILLGGSAIPAPLIEEAQQ-YGLPIYLSYGMTETCsqvTTATPEMLHAR--P 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 388 SVGTPLPGVEVCIATetlkdgarsytihaqgdesntqvtPGLEGQeGELLVRGPSVFREYWNrPRETEDAFTPDGWFKTG 467
Cdd:TIGR01923 272 DVGRPLAGREIKIKV------------------------DNKEGH-GEIMVKGANLMKGYLY-QGELTPAFEQQGWFNTG 325
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 468 DTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEmlSVKDLKD 546
Cdd:TIGR01923 326 DIGELDgEGFLYVLGRRD-DLIISGGENIYPEEIETVLYQHPGIQEAVVVPKPDAEWGQVPVAYIVSESDI--SQAKLIA 402
|
490 500 510
....*....|....*....|....*....|....
gi 2452540125 547 WARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:TIGR01923 403 YLTEKLAKYKVPIAFEKLDELPYNASGKILRNQL 436
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
64-584 |
4.41e-52 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 187.02 E-value: 4.41e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIidqngehTYRDLLsqslRLSQEICRALDCSSRdLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEl 143
Cdd:PRK05852 40 DRIAI-------SYRDLA----RLVDDLAGQLTRSGL-LPGDRVALRMGSNAEFVVALLAASRADLVVVPLDPALPIAE- 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 eyviQDSQSALVIAAEEYVGKITPSAEKLG-VPVLPLL------RSCSDGSAS---HPAVEEGPLTTC-SSWKNRGAMII 212
Cdd:PRK05852 107 ----QRVRSQAAGARVVLIDADGPHDRAEPtTRWWPLTvnvggdSGPSGGTLSvhlDAATEPTPATSTpEGLRPDDAMIM 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 213 YTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATcVMLP---EFNAQMVWK 289
Cdd:PRK05852 183 FTGGTTGLPKMVPWTHANIASSVRAIITGYRLSPRDATVAVMPLYHGHGLIAALLATLASGGA-VLLPargRFSAHTFWD 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 290 KLLSSQApriSVFMAVPTIYAKLVEyydkhffQPQVQDFVRAfcQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGM 369
Cdd:PRK05852 262 DIKAVGA---TWYTAVPTIHQILLE-------RAATEPSGRK--PAALRFIRSCSAPLTAETAQALQTEFAAPVVCAFGM 329
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 370 TEIGMALSNplhgVRIPGSVGTPLPGVEVCIATETlkDGARSYTIHAQGDEsntqVTPGlegQEGELLVRGPSVFREYWN 449
Cdd:PRK05852 330 TEATHQVTT----TQIEGIGQTENPVVSTGLVGRS--TGAQIRIVGSDGLP----LPAG---AVGEVWLRGTTVVRGYLG 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 450 RPRETEDAFTpDGWFKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVS 528
Cdd:PRK05852 397 DPTITAANFT-DGWLRTGDLGSLSaAGDLSIRGRIK-ELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVA 474
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 529 AVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQRF 584
Cdd:PRK05852 475 AVIVPRESAPPTAEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRRAVAEQF 530
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
105-584 |
1.12e-51 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 185.75 E-value: 1.12e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 105 ERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALVI--------AAEeyVGKITPSAEKLGV-- 174
Cdd:PRK07786 68 DRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPEIAFLVSDCGAHVVVteaalapvATA--VRDIVPLLSTVVVag 145
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 175 -----PVLPLLRSCSDGSASHPAV---EEGPlttcsswknrgAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKK 246
Cdd:PRK07786 146 gssddSVLGYEDLLAEAGPAHAPVdipNDSP-----------ALIMYTSGTTGRPKGAVLTHANLTGQAMTCLRTNGADI 214
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 247 E-DVILHVLPLHHVHGVINkLLCPLWVGATCVMLP--EFNAQMVWKKLLSSQAprISVFMaVPTiyaklveyydkhffqp 323
Cdd:PRK07786 215 NsDVGFVGVPLFHIAGIGS-MLPGLLLGAPTVIYPlgAFDPGQLLDVLEAEKV--TGIFL-VPA---------------- 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 324 QVQdfvrAFCQE------NIRLMVSGSAALPVP--VL-EKWKSITGHTLLERYGMTEIG----MALSNplHGVRIPGSVG 390
Cdd:PRK07786 275 QWQ----AVCAEqqarprDLALRVLSWGAAPASdtLLrQMAATFPEAQILAAFGQTEMSpvtcMLLGE--DAIRKLGSVG 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 391 TPLPGVEVCIATETLKDgarsytihaqgdesntqVTPGlegQEGELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTA 470
Cdd:PRK07786 349 KVIPTVAARVVDENMND-----------------VPVG---EVGEIVYRAPTLMSGYWNNPEATAEAFA-GGWFHSGDLV 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 471 AY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKG-EMLSVKDLKDWA 548
Cdd:PRK07786 408 RQdEEGYVWVVDRKK-DMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRNDdAALTLEDLAEFL 486
|
490 500 510
....*....|....*....|....*....|....*.
gi 2452540125 549 RDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQRF 584
Cdd:PRK07786 487 TDRLARYKHPKALEIVDALPRNPAGKVLKTELRERY 522
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
74-510 |
2.58e-51 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 183.03 E-value: 2.58e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 74 EHTYRDLLSQSLRLSQ--EICRALdcssrdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQ 151
Cdd:cd05914 7 PLTYKDLADNIAKFALllKINGVG-------TGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSE 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 152 SALVIAAEEyvgkitpsaEKLgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTGRPKGVLSTHENV 231
Cdd:cd05914 80 AKAIFVSDE---------DDV------------------------------------ALINYTSGTTGNSKGVMLTYRNI 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 232 QAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEfnaqMVWKKLLSSQAPRISVFMAVPTIY-- 309
Cdd:cd05914 115 VSNVDGVKEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDK----IPSAKIIALAFAQVTPTLGVPVPLvi 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 310 --------------AKLVEYYDKHFFQPQVQDFVRAFCQE----NIRLMVSGSAALPVPVLEKWKSItGHTLLERYGMTE 371
Cdd:cd05914 191 ekifkmdiipkltlKKFKFKLAKKINNRKIRKLAFKKVHEafggNIKEFVIGGAKINPDVEEFLRTI-GFPYTIGYGMTE 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 372 IGMALS-NPLHGVRIpGSVGTPLPGVEVCIATetlkdgarsytihaqgdesntqvtPGLEGQEGELLVRGPSVFREYWNR 450
Cdd:cd05914 270 TAPIISySPPNRIRL-GSAGKVIDGVEVRIDS------------------------PDPATGEGEIIVRGPNVMKGYYKN 324
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2452540125 451 PRETEDAFTPDGWFKTGDTAAYKDGVY-WIKGRTSVDIIKNGGFKISALEVERHLLAHPHI 510
Cdd:cd05914 325 PEATAEAFDKDGWFHTGDLGKIDAEGYlYIRGRKKEMIVLSSGKNIYPEEIEAKINNMPFV 385
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
211-577 |
1.61e-50 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 177.31 E-value: 1.61e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 211 IIYTSGTTGRPKGVLSTHENvqavTTGLVEKW----EWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQM 286
Cdd:cd17638 5 IMFTSGTTGRSKGVMCAHRQ----TLRAAAAWadcaDLTEDDRYLIINPFFHTFGYKAGIVACLLTGATVVPVAVFDVDA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 287 VWKKLLSSqapRISVFMAVPTIYAKLVEYydkhffqPQVQDFVRAfcqeNIRLMVSGSAALPVPVLEKWKSITG-HTLLE 365
Cdd:cd17638 81 ILEAIERE---RITVLPGPPTLFQSLLDH-------PGRKKFDLS----SLRAAVTGAATVPVELVRRMRSELGfETVLT 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 366 RYGMTEIGMA-LSNPLHG-VRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgdesntqvtpglegQEGELLVRGPSV 443
Cdd:cd17638 147 AYGLTEAGVAtMCRPGDDaETVATTCGRACPGFEVRIA------------------------------DDGEVLVRGYNV 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 444 FREYWNRPRETEDAFTPDGWFKTGDTAAYKD-GVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMV 522
Cdd:cd17638 197 MQGYLDDPEATAEAIDADGWLHTGDVGELDErGYLRITDRLK-DMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDER 275
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*
gi 2452540125 523 WGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNK 577
Cdd:cd17638 276 MGEVGKAFVVARPGVTLTEEDVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
66-586 |
2.74e-50 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 181.26 E-value: 2.74e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 66 IAIIDQNGEH-TYRDLLSQSLRLSQEIcRALDcssrdLKE-ERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:PRK08276 2 AVIMAPSGEVvTYGELEARSNRLAHGL-RALG-----LREgDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEI 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAAEEYVGKITPSAEKL--GVPVLPLLRSCSDGSASHPAVEEGPLTTCSSWKNRGAMIIYTSGTTGRP 221
Cdd:PRK08276 76 AYIVDDSGAKVLIVSAALADTAAELAAELpaGVPLLLVVAGPVPGFRSYEEALAAQPDTPIADETAGADMLYSSGTTGRP 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 222 KGVLSTHENVQ-----AVTTGLVEKWEWKKED-VILHVLPLHHVhGVINKLLCPLWVGATCVMLPEFNAQMVwkkLLSSQ 295
Cdd:PRK08276 156 KGIKRPLPGLDpdeapGMMLALLGFGMYGGPDsVYLSPAPLYHT-APLRFGMSALALGGTVVVMEKFDAEEA---LALIE 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 296 APRISVFMAVPTIYAKLVeyydkhffqpQVQDFVRA-FCQENIRLMVSGSAALPVPVleKWKSIT--GHTLLERYGMTE- 371
Cdd:PRK08276 232 RYRVTHSQLVPTMFVRML----------KLPEEVRArYDVSSLRVAIHAAAPCPVEV--KRAMIDwwGPIIHEYYASSEg 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 372 IGMALSNPLHGVRIPGSVGTPLPG-VEVCiatetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFREYWNR 450
Cdd:PRK08276 300 GGVTVITSEDWLAHPGSVGKAVLGeVRIL-------------------DEDGNELPPG---EIGTVYFEMDGYPFEYHND 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 451 PRETEDAFTPDGWFKTGDTAaY--KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVS 528
Cdd:PRK08276 358 PEKTAAARNPHGWVTVGDVG-YldEDGYLYLTDRKS-DMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVK 435
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2452540125 529 AVVQLRKGEMLSV---KDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQRFYP 586
Cdd:PRK08276 436 AVVQPADGADAGDalaAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLRDRYWE 496
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
64-580 |
1.57e-49 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 178.86 E-value: 1.57e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEH--TYRDLLSQSLRLSQeicrALDcsSRDLK-EERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPV 140
Cdd:cd05923 16 DACAIADPARGLrlTYSELRARIEAVAA----RLH--ARGLRpGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 141 TELEYVIQDSQSALVI---AAEEYVGKITPSAEKLGVPVLPLLRSCSDGSASHPAVEEGPLTTcsswknrgAMIIYTSGT 217
Cdd:cd05923 90 AELAELIERGEMTAAViavDAQVMDAIFQSGVRVLALSDLVGLGEPESAGPLIEDPPREPEQP--------AFVFYTSGT 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 218 TGRPKGVLSTH----ENVQAVTTglVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWKKLls 293
Cdd:cd05923 162 TGLPKGAVIPQraaeSRVLFMST--QAGLRHGRHNVVLGLMPLYHVIGFFAVLVAALALDGTYVVVEEFDPADALKLI-- 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 294 sQAPRISVFMAVPTIYAKLVEYYDkhfFQPQVQDFVRafcqeniRLMVSGsAALPVPVLEKWKSITGHTLLERYGMTEIG 373
Cdd:cd05923 238 -EQERVTSLFATPTHLDALAAAAE---FAGLKLSSLR-------HVTFAG-ATMPDAVLERVNQHLPGEKVNIYGTTEAM 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 374 MALSNPlhGVRiPGSVGTPLPGVEVCIAtetlKDGARSYTIHAQGDEsntqvtpglegqeGELLVR--GPSVFREYWNRP 451
Cdd:cd05923 306 NSLYMR--DAR-TGTEMRPGFFSEVRIV----RIGGSPDEALANGEE-------------GELIVAaaADAAFTGYLNQP 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 452 RETEDAFTpDGWFKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAV 530
Cdd:cd05923 366 EATAKKLQ-DGWYRTGDVGYVDpSGDVRILGRVD-DMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTAC 443
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 2452540125 531 VQLRKGEmLSVKDLKDWARDT-MAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05923 444 VVPREGT-LSADELDQFCRASeLADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
207-584 |
2.00e-49 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 174.06 E-value: 2.00e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 207 RGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCpLWVGATCVMLPEfnAQM 286
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWLLSLPLYHVGGLAILVRS-LLAGAELVLLER--NQA 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 287 VWKKLLSSQAPRISVfmaVPTIYAKLVEYydkHFFQPQVqdfvrafcqENIRLMVSGSAALPVPVLEK-----WKSITGh 361
Cdd:cd17630 78 LAEDLAPPGVTHVSL---VPTQLQRLLDS---GQGPAAL---------KSLRAVLLGGAPIPPELLERaadrgIPLYTT- 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 362 tllerYGMTEIGMALSNPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgdesntqvtpglegQEGELLVRGP 441
Cdd:cd17630 142 -----YGMTETASQVATKRPDGFGRGGVGVLLPGRELRIV------------------------------EDGEIWVGGA 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 442 SVFREYWNRPREteDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPD 520
Cdd:cd17630 187 SLAMGYLRGQLV--PEFNEDGWFTTKDLGELhADGRLTVLGRAD-NMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPD 263
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2452540125 521 MVWGQRVSAVVQLRKGemLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQRF 584
Cdd:cd17630 264 EELGQRPVAVIVGRGP--ADPAELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
63-580 |
2.58e-49 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 177.87 E-value: 2.58e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 63 GDKIAIIDQNGEHTYRDLLSQSLRLSQEICRALDCSsrdlkEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTE 142
Cdd:cd12116 1 PDATAVRDDDRSLSYAELDERANRLAARLRARGVGP-----GDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADR 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 143 LEYVIQDSQSALVIAAEEyvgkitpSAEKLGVPVLPLLRSCSDGSASHPAVEEGPLTtcsswkNRGAMIIYTSGTTGRPK 222
Cdd:cd12116 76 LRYILEDAEPALVLTDDA-------LPDRLPAGLPVLLLALAAAAAAPAAPRTPVSP------DDLAYVIYTSGSTGRPK 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 223 GVLSTHENVQAVTTGLVEKWEWKKEDVILHVLP-------LhhvhgvinKLLCPLWVGATCVMLPEFNAQ--MVWKKLLS 293
Cdd:cd12116 143 GVVVSHRNLVNFLHSMRERLGLGPGDRLLAVTTyafdislL--------ELLLPLLAGARVVIAPRETQRdpEALARLIE 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 294 sqAPRISVFMAVPTIYAKLVEYydkhffQPQVQDFVRAFCqenirlmvsGSAALPVPVLEKWKSITGhTLLERYGMTEI- 372
Cdd:cd12116 215 --AHSITVMQATPATWRMLLDA------GWQGRAGLTALC---------GGEALPPDLAARLLSRVG-SLWNLYGPTETt 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 373 ---GMALSNPLHGVrIPgsVGTPLPGVEVciatetlkdgarsYTIHAQGDesntqvtPGLEGQEGELLVRGPSVFREYWN 449
Cdd:cd12116 277 iwsTAARVTAAAGP-IP--IGRPLANTQV-------------YVLDAALR-------PVPPGVPGELYIGGDGVAQGYLG 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 450 RPRETEDAFTPDG-------WFKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDM 521
Cdd:cd12116 334 RPALTAERFVPDPfagpgsrLYRTGDLVRRRaDGRLEYLGRAD-GQVKIRGHRIELGEIEAALAAHPGVAQAAVVVREDG 412
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 2452540125 522 VwGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd12116 413 G-DRRLVAYVVLKAGAAPDAAALRAHLRATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
64-580 |
3.61e-49 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 178.44 E-value: 3.61e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEIcraldcSSRDLKE-ERISFLCPNDASYVVAQWASWMSGGIAV---PLYKKHP 139
Cdd:TIGR03098 15 DATALVHHDRTLTYAALSERVLALASGL------RGLGLARgERVAIYLDKRLETVTAMFGAALAGGVFVpinPLLKAEQ 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 140 VtelEYVIQDSQSALVIAAEEYVGKITPSAeklgvPVLPLLRSCSDGSASHPAVEEGPLTTCSSWKN-----------RG 208
Cdd:TIGR03098 89 V---AHILADCNVRLLVTSSERLDLLHPAL-----PGCHDLRTLIIVGDPAHASEGHPGEEPASWPKllalgdadpphPV 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 -----AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGvINKLLCPLWVGATCVMLpefN 283
Cdd:TIGR03098 161 idsdmAAILYTSGSTGRPKGVVLSHRNLVAGAQSVATYLENRPDDRLLAVLPLSFDYG-FNQLTTAFYVGATVVLH---D 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 284 AQMVWKKLLSSQAPRISVFMAVPTIYAKLVEyydkhfFQPQVQDFvrafcqENIRLMVSGSAALPVPVLEKWKSITGHT- 362
Cdd:TIGR03098 237 YLLPRDVLKALEKHGITGLAAVPPLWAQLAQ------LDWPESAA------PSLRYLTNSGGAMPRATLSRLRSFLPNAr 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 363 LLERYGMTEIGMALSNPLHGV-RIPGSVGTPLPGVEVCIAtetlkdgarsytiHAQGDESntqvtpgLEGQEGELLVRGP 441
Cdd:TIGR03098 305 LFLMYGLTEAFRSTYLPPEEVdRRPDSIGKAIPNAEVLVL-------------REDGSEC-------APGEEGELVHRGA 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 442 SVFREYWNRPRETEDAFTP----DGWFKTGDTAAY--------KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPH 509
Cdd:TIGR03098 365 LVAMGYWNDPEKTAERFRPlppfPGELHLPELAVWsgdtvrrdEEGFLYFVGRRD-EMIKTSGYRVSPTEVEEVAYATGL 443
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2452540125 510 ITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:TIGR03098 444 VAEAVAFGVPDPTLGQAIVLVVTPPGGEELDRAALLAECRARLPNYMVPALIHVRQALPRNANGKIDRKAL 514
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
209-580 |
2.25e-48 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 172.28 E-value: 2.25e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLP------EF 282
Cdd:cd05944 5 AAYFHTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGAHVVLAGpagyrnPG 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 283 NAQMVWKKLlssQAPRISVFMAVPTIYAKLVEyydkhffQPQVQDFvrafcqENIRLMVSGSAALPVPVLEKWKSITGHT 362
Cdd:cd05944 85 LFDNFWKLV---ERYRITSLSTVPTVYAALLQ-------VPVNADI------SSLRFAMSGAAPLPVELRARFEDATGLP 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 363 LLERYGMTEIGMALS-NPLHGVRIPGSVGTPLPGVEVCIATEtlkDGARSYTIHAQGDESntqvtpglegqeGELLVRGP 441
Cdd:cd05944 149 VVEGYGLTEATCLVAvNPPDGPKRPGSVGLRLPYARVRIKVL---DGVGRLLRDCAPDEV------------GEICVAGP 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 442 SVFREYWNRPREtEDAFTPDGWFKTGDTAAY-KDGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPD 520
Cdd:cd05944 214 GVFGGYLYTEGN-KNAFVADGWLNTGDLGRLdADGYLFITGR-AKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPD 291
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2452540125 521 MVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPY-TIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05944 292 AHAGELPVAYVQLKPGAVVEEEELLAWARDHVPERaAVPKHIEVLEELPVTAVGKVFKPAL 352
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
103-580 |
3.56e-48 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 173.84 E-value: 3.56e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 103 KEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALVIAAEEYVGKITPsaeklgvpvlpllrs 182
Cdd:cd05969 24 KGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTEELYERTDP--------------- 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 183 csdgsashpaveegplttcsswkNRGAMIIYTSGTTGRPKGVLSTHENV--QAVTTglveKW--EWKKEDVILHVLPLHH 258
Cdd:cd05969 89 -----------------------EDPTLLHYTSGTTGTPKGVLHVHDAMifYYFTG----KYvlDLHPDDIYWCTADPGW 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 259 VHGVINKLLCPLWVGATCVMLP-EFNAQMvWKKLLssQAPRISVFMAVPTIYAKLVEYYDkhffqpqvqDFVRAFCQENI 337
Cdd:cd05969 142 VTGTVYGIWAPWLNGVTNVVYEgRFDAES-WYGII--ERVKVTVWYTAPTAIRMLMKEGD---------ELARKYDLSSL 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 338 RLMVSGSAALPvPVLEKW-KSITGHTLLERYGMTEIG-MALSNPLHGVRIPGSVGTPLPGVEVCIatetlkdgarsytIH 415
Cdd:cd05969 210 RFIHSVGEPLN-PEAIRWgMEVFGVPIHDTWWQTETGsIMIANYPCMPIKPGSMGKPLPGVKAAV-------------VD 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 416 AQGDEsntqvTPglEGQEGELLVRG--PSVFREYWNRPRETEDAFtPDGWFKTGDTAaYKD--GVYWIKGRTSvDIIKNG 491
Cdd:cd05969 276 ENGNE-----LP--PGTKGILALKPgwPSMFRGIWNDEERYKNSF-IDGWYLTGDLA-YRDedGYFWFVGRAD-DIIKTS 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 492 GFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVK---DLKDWARDTMAPYTIPTELIVVEEIP 568
Cdd:cd05969 346 GHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDElkeEIINFVRQKLGAHVAPREIEFVDNLP 425
|
490
....*....|..
gi 2452540125 569 RNQMGKVNKKEL 580
Cdd:cd05969 426 KTRSGKIMRRVL 437
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
64-584 |
5.15e-48 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 176.38 E-value: 5.15e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRlsqeiCRALDCSSRDLKEERISFLCPNDASYVVAQWASWMSGGIAVP---LYKKHpv 140
Cdd:PRK06178 48 QRPAIIFYGHVITYAELDELSDR-----FAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPvspLFREH-- 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 141 tELEYVIQDSQSALVIAAEEYV--------------------GKITPSAEKLGVP---------------VLPLLRSCSD 185
Cdd:PRK06178 121 -ELSYELNDAGAEVLLALDQLApvveqvraetslrhvivtslADVLPAEPTLPLPdslraprlaaagaidLLPALRACTA 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 186 GSASHPAVEEGPlttcsswknrgAMIIYTSGTTGRPKGVLSTHENV--QAVTTGLVEKWEwKKEDVILHVLPLHHVHGVI 263
Cdd:PRK06178 200 PVPLPPPALDAL-----------AALNYTGGTTGMPKGCEHTQRDMvyTAAAAYAVAVVG-GEDSVFLSFLPEFWIAGEN 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 264 NKLLCPLWVGATCVMLPEFNAQmvwkkllssqapriSVFMAVP----TIYAKLVEYYDKHFFQPQVQDF-------VRAf 332
Cdd:PRK06178 268 FGLLFPLFSGATLVLLARWDAV--------------AFMAAVEryrvTRTVMLVDNAVELMDHPRFAEYdlsslrqVRV- 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 333 cqenirlmVSGSAALPVPVLEKWKSITGHTLLE-RYGMTE--------IGMA------LSNPLHgvripgsVGTPLPGVE 397
Cdd:PRK06178 333 --------VSFVKKLNPDYRQRWRALTGSVLAEaAWGMTEthtcdtftAGFQdddfdlLSQPVF-------VGLPVPGTE 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 398 VCIAtetlkdgarsytihaqgDESNTQVTPglEGQEGELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTAAY-KDGV 476
Cdd:PRK06178 398 FKIC-----------------DFETGELLP--LGAEGEIVVRTPSLLKGYWNKPEATAEALR-DGWLHTGDIGKIdEQGF 457
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 477 YWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYT 556
Cdd:PRK06178 458 LHYLGRRK-EMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGADLTAAALQAWCRENMAVYK 536
|
570 580
....*....|....*....|....*...
gi 2452540125 557 IPtELIVVEEIPRNQMGKVNKKELLQRF 584
Cdd:PRK06178 537 VP-EIRIVDALPMTATGKVRKQDLQALA 563
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
64-580 |
1.59e-47 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 173.53 E-value: 1.59e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLlSQSLRLSQEICRALDCSSRDLkeerISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:PRK06145 17 DRAALVYRDQEISYAEF-HQRILQAAGMLHARGIGQGDV----VALLMKNSAAFLELAFAASYLGAVFLPINYRLAADEV 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLPLLRSCSDGSASHPAVEEGPLTTCSSWKnrgamIIYTSGTTGRPKG 223
Cdd:PRK06145 92 AYILGDAGAKLLLVDEEFDAIVALETPKIVIDAAAQADSRRLAQGGLEIPPQAAVAPTDLVR-----LMYTSGTTDRPKG 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 224 VLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVwkkLLSSQAPRISVFM 303
Cdd:PRK06145 167 VMHSYGNLHWKSIDHVIALGLTASERLLVVGPLYHVGAFDLPGIAVLWVGGTLRIHREFDPEAV---LAAIERHRLTCAW 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 304 AVPTIYAKLVEYYDKHFFQpqvqdfvrafcQENIRLMVSGSAALPVPVLEKWKSI-TGHTLLERYGMTEI--GMALSNPL 380
Cdd:PRK06145 244 MAPVMLSRVLTVPDRDRFD-----------LDSLAWCIGGGEKTPESRIRDFTRVfTRARYIDAYGLTETcsGDTLMEAG 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 381 HGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFREYWNRPRETEDAFTp 460
Cdd:PRK06145 313 REIEKIGSTGRALAHVEIRIA-----------------DGAGRWLPPN---MKGEICMRGPKVTKGYWKDPEKTAEAFY- 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 461 DGWFKTGDTAAYKD-GVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEML 539
Cdd:PRK06145 372 GDWFRSGDVGYLDEeGFLYLTDRKK-DMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGATL 450
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 2452540125 540 SVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK06145 451 TLEALDRHCRQRLASFKVPRQLKVRDELPRNPSGKVLKRVL 491
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
76-515 |
1.99e-47 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 170.91 E-value: 1.99e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSqeicRALDCSSRDLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALV 155
Cdd:TIGR01733 1 TYRELDERANRLA----RHLRAAGGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLL 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 156 IAAEEYvgkitpSAEKLGVPVLPLLRSCSDGSASHPAVEEGPLTTCSSWKNRgAMIIYTSGTTGRPKGVLSTHENVQAVT 235
Cdd:TIGR01733 77 LTDSAL------ASRLAGLVLPVILLDPLELAALDDAPAPPPPDAPSGPDDL-AYVIYTSGSTGRPKGVVVTHRSLVNLL 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 236 TGLVEKWEWKKEDVILHVLPLHHVHGVInKLLCPLWVGATCVMLPEFNAQMVWKKLLSSQAPR-ISVFMAVPTIYAKLVE 314
Cdd:TIGR01733 150 AWLARRYGLDPDDRVLQFASLSFDASVE-EIFGALLAGATLVVPPEDEERDDAALLAALIAEHpVTVLNLTPSLLALLAA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 315 yydkhffqpqvqdfVRAFCQENIRLMVSGSAALPVPVLEKWKSITGHT-LLERYGMTEI-GMALSNPLHGVRIPG----S 388
Cdd:TIGR01733 229 --------------ALPPALASLRLVILGGEALTPALVDRWRARGPGArLINLYGPTETtVWSTATLVDPDDAPRespvP 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 389 VGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGLegqEGELLVRGPSVFREYWNRPRETEDAFTPDG------ 462
Cdd:TIGR01733 295 IGRPLANTRLYVL-----------------DDDLRPVPVGV---VGELYIGGPGVARGYLNRPELTAERFVPDPfaggdg 354
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 463 --WFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAV 515
Cdd:TIGR01733 355 arLYRTGDLVRYlPDGNLEFLGRID-DQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
42-577 |
2.16e-47 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 174.58 E-value: 2.16e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 42 QTTGAAYSSDVApvftkalAFGDKIAIIdqngehtyrdLLSQSLRLS-QEICRALDCSSRDL------KEERISFLCPND 114
Cdd:PRK12583 18 QTIGDAFDATVA-------RFPDREALV----------VRHQALRYTwRQLADAVDRLARGLlalgvqPGDRVGIWAPNC 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 115 ASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALVIAAEEY--------VGKITPS-----AEKLGVPVLPLLR 181
Cdd:PRK12583 81 AEWLLTQFATARIGAILVNINPAYRASELEYALGQSGVRWVICADAFktsdyhamLQELLPGlaegqPGALACERLPELR 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 182 -------SCSDGSASHPAVE-EGPLTTCSSWKNRGAM--------IIYTSGTTGRPKGVLSTHENV------QAVTTGLV 239
Cdd:PRK12583 161 gvvslapAPPPGFLAWHELQaRGETVSREALAERQASldrddpinIQYTSGTTGFPKGATLSHHNIlnngyfVAESLGLT 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 240 EkwewkkEDVILHVLPLHHVHG-VINKLLCpLWVGAtCVMLP--EFNAQMVwkkLLSSQAPRISVFMAVPTIYAKLVEYY 316
Cdd:PRK12583 241 E------HDRLCVPVPLYHCFGmVLANLGC-MTVGA-CLVYPneAFDPLAT---LQAVEEERCTALYGVPTMFIAELDHP 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 317 DKHFFqpqvqDFvrafcqENIRLMVSGSAALPVPVLEK-WKSITGHTLLERYGMTEIG-----MALSNPLHgvRIPGSVG 390
Cdd:PRK12583 310 QRGNF-----DL------SSLRTGIMAGAPCPIEVMRRvMDEMHMAEVQIAYGMTETSpvslqTTAADDLE--RRVETVG 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 391 TPLPGVEVCIatetlkdgarsytIHAQGdesntQVTPglEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTA 470
Cdd:PRK12583 377 RTQPHLEVKV-------------VDPDG-----ATVP--RGEIGELCTRGYSVMKGYWNNPEATAESIDEDGWMHTGDLA 436
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 471 AY-KDGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWAR 549
Cdd:PRK12583 437 TMdEQGYVRIVGR-SKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGVPDEKYGEEIVAWVRLHPGHAASEEELREFCK 515
|
570 580
....*....|....*....|....*...
gi 2452540125 550 DTMAPYTIPTELIVVEEIPRNQMGKVNK 577
Cdd:PRK12583 516 ARIAHFKVPRYFRFVDEFPMTVTGKVQK 543
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
62-583 |
2.24e-46 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 170.99 E-value: 2.24e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 62 FGDKIAIIDQNGEHTYRDLLSQSLRLSQEICRALDCssrdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVT 141
Cdd:PRK07470 20 FPDRIALVWGDRSWTWREIDARVDALAAALAARGVR-----KGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNFRQTPD 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 142 ELEYVIQDSQSALVI--------------AAEEYVGKITPSAEKLGVPVLPLLRSCSDGSASHPAVE-EGPLttcssWkn 206
Cdd:PRK07470 95 EVAYLAEASGARAMIchadfpehaaavraASPDLTHVVAIGGARAGLDYEALVARHLGARVANAAVDhDDPC-----W-- 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 207 rgamIIYTSGTTGRPKGVLSTHENVQAVTT--------GLVEkwewkkEDVILHVLPLHHVHGVinKLLCPLWVGATCVM 278
Cdd:PRK07470 168 ----FFFTSGTTGRPKAAVLTHGQMAFVITnhladlmpGTTE------QDASLVVAPLSHGAGI--HQLCQVARGAATVL 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 279 LP--EFNAQMVWKKLlssQAPRISVFMAVPTIYAKLVEYydkhffqPQVQdfvrAFCQENIRLMVSGSAALPVPVLEKWK 356
Cdd:PRK07470 236 LPseRFDPAEVWALV---ERHRVTNLFTVPTILKMLVEH-------PAVD----RYDHSSLRYVIYAGAPMYRADQKRAL 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 357 SITGHTLLERYGMTEIGMA---LSNPLH------GVRIpGSVGTPLPGVEVCIatetlkdgarsytihaQGDESNtQVTP 427
Cdd:PRK07470 302 AKLGKVLVQYFGLGEVTGNitvLPPALHdaedgpDARI-GTCGFERTGMEVQI----------------QDDEGR-ELPP 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 428 GlegQEGELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTAaYKD--GVYWIKGRTSvDIIKNGGFKISALEVERHLL 505
Cdd:PRK07470 364 G---ETGEICVIGPAVFAGYYNNPEANAKAFR-DGWFRTGDLG-HLDarGFLYITGRAS-DMYISGGSNVYPREIEEKLL 437
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 506 AHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKK----ELL 581
Cdd:PRK07470 438 THPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVDEAELLAWLDGKVARYKLPKRFFFWDALPKSGYGKITKKmvreELE 517
|
..
gi 2452540125 582 QR 583
Cdd:PRK07470 518 ER 519
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
105-581 |
1.18e-45 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 167.62 E-value: 1.18e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 105 ERISFLCPNDASYVVAQWASWMSGG----IAVPLYKKHPVTELEYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLPL- 179
Cdd:cd05922 19 ERVVLILPNRFTYIELSFAVAYAGGrlglVFVPLNPTLKESVLRYLVADAGGRIVLADAGAADRLRDALPASPDPGTVLd 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 180 ---LRSCSDGSASHPAVEEGPlttcsswknrgAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPL 256
Cdd:cd05922 99 adgIRAARASAPAHEVSHEDL-----------ALLLYTSGSTGSPKLVRLSHQNLLANARSIAEYLGITADDRALTVLPL 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 257 HHVHGvINKLLCPLWVGATCVMLPEFN-AQMVWKKLlssQAPRISVFMAVPTIYAKLveyyDKHFFQPQVQDFVRAFCQe 335
Cdd:cd05922 168 SYDYG-LSVLNTHLLRGATLVLTNDGVlDDAFWEDL---REHGATGLAGVPSTYAML----TRLGFDPAKLPSLRYLTQ- 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 336 nirlmvSGSAALPVPVLEKWKSITGHTLLERYGMTEI--GMALSNPLHGVRIPGSVGTPLPGVEVCIATEtlkDGARSYT 413
Cdd:cd05922 239 ------AGGRLPQETIARLRELLPGAQVYVMYGQTEAtrRMTYLPPERILEKPGSIGLAIPGGEFEILDD---DGTPTPP 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 414 ihaqgdesntqvtpgleGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGG 492
Cdd:cd05922 310 -----------------GEPGEIVHRGPNVMKGYWNDPPYRRKEGRGGGVLHTGDLARRdEDGFLFIVGRRD-RMIKLFG 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 493 FKISALEVERHLLAHPHITDVAVIGPPDMVwGQRVSAVVQLRKGemLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQM 572
Cdd:cd05922 372 NRISPTEIEAAARSIGLIIEAAAVGLPDPL-GEKLALFVTAPDK--IDPKDVLRSLAERLPPYKVPATVRVVDELPLTAS 448
|
....*....
gi 2452540125 573 GKVNKKELL 581
Cdd:cd05922 449 GKVDYAALR 457
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
46-580 |
5.30e-44 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 164.99 E-value: 5.30e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 46 AAYSSdVAPVFTKAL-AFGDKIAIIDQNGEHTYRDLLsqslRLSQEICRALDCSSRDLKEERISFLCPNDASYVVAQWAS 124
Cdd:PRK12492 21 AAYKS-VVEVFERSCkKFADRPAFSNLGVTLSYAELE----RHSAAFAAYLQQHTDLVPGDRIAVQMPNVLQYPIAVFGA 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 125 WMSGGIAV---PLYK----KH------------------------PVTELEYVIQDSQSALVIAAEEY-VGKITPSAEKL 172
Cdd:PRK12492 96 LRAGLIVVntnPLYTaremRHqfkdsgaralvylnmfgklvqevlPDTGIEYLIEAKMGDLLPAAKGWlVNTVVDKVKKM 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 173 gVPVLPLLRSCSDGSASHPAVEEGPLTTCSSWKNRgAMIIYTSGTTGRPKGVLSTH----ENVQAVTTGLVEKWE----- 243
Cdd:PRK12492 176 -VPAYHLPQAVPFKQALRQGRGLSLKPVPVGLDDI-AVLQYTGGTTGLAKGAMLTHgnlvANMLQVRACLSQLGPdgqpl 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 244 WKK-EDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPefNAQMVWKKLLSSQAPRISVFMAVPTIYAKLVEYydkhffq 322
Cdd:PRK12492 254 MKEgQEVMIAPLPLYHIYAFTANCMCMMVSGNHNVLIT--NPRDIPGFIKELGKWRFSALLGLNTLFVALMDH------- 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 323 PQVQ--DFvrafcqENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIG-MALSNPLHGVRIPGSVGTPLPGVevc 399
Cdd:PRK12492 325 PGFKdlDF------SALKLTNSGGTALVKATAERWEQLTGCTIVEGYGLTETSpVASTNPYGELARLGTVGIPVPGT--- 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 400 iateTLKdgarsyTIHAQGDESNTqvtpgleGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYW 478
Cdd:PRK12492 396 ----ALK------VIDDDGNELPL-------GERGELCIKGPQVMKGYWQQPEATAEALDAEGWFKTGDIAVIdPDGFVR 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 479 IKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEmLSVKDLKDWARDTMAPYTIP 558
Cdd:PRK12492 459 IVDRKK-DLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVVARDPG-LSVEELKAYCKENFTGYKVP 536
|
570 580
....*....|....*....|..
gi 2452540125 559 TELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK12492 537 KHIVLRDSLPMTPVGKILRREL 558
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
59-580 |
8.08e-44 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 164.33 E-value: 8.08e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcSSRDLKE-ERISFLCPNDASYVVAQWASWMSGGIAVPLYKK 137
Cdd:PRK07788 59 ARRAPDRAALIDERGTLTYAELDEQSNALA----RGL--LALGVRAgDGVAVLARNHRGFVLALYAAGKVGARIILLNTG 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 138 HPVTELEYVIQDSQSALVIAAEEYVGKITPSAEKLGVpVLPLLRSCSDGSASHPAVE----------EGPLTTcssWKNR 207
Cdd:PRK07788 133 FSGPQLAEVAAREGVKALVYDDEFTDLLSALPPDLGR-LRAWGGNPDDDEPSGSTDEtlddliagssTAPLPK---PPKP 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 208 GAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCpLWVGATCVMLPEFNAQMV 287
Cdd:PRK07788 209 GGIVILTSGTTGTPKGAPRPEPSPLAPLAGLLSRVPFRAGETTLLPAPMFHATGWAHLTLA-MALGSTVVLRRRFDPEAT 287
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 288 WKKLlssQAPRISVFMAVPTIYAKLVEYYDKHFFQPQVQdfvrafcqeNIRLMVSGSAALPVPVLEKWKSITGHTLLERY 367
Cdd:PRK07788 288 LEDI---AKHKATALVVVPVMLSRILDLGPEVLAKYDTS---------SLKIIFVSGSALSPELATRALEAFGPVLYNLY 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 368 GMTEIGMA-LSNPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFRE 446
Cdd:PRK07788 356 GSTEVAFAtIATPEDLAEAPGTVGRPPKGVTVKIL-----------------DENGNEVPRG---VVGRIFVGNGFPFEG 415
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 447 YWN-RPRETEDaftpdGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWG 524
Cdd:PRK07788 416 YTDgRDKQIID-----GLLSSGDVGYFdEDGLLFVDGRDD-DMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFG 489
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 525 QRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK07788 490 QRLRAFVVKAPGAALDEDAIKDYVRDNLARYKVPRDVVFLDELPRNPTGKVLKREL 545
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
62-580 |
1.51e-43 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 163.78 E-value: 1.51e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 62 FGDKIAIIDQNGEHTYRDLLSQS----LRLSQEIcraldcssrDLKE-ERISFLCPNDASYVVAQWASWMSGGIAV---P 133
Cdd:PRK05677 37 FADKPAFSNLGKTLTYGELYKLSgafaAWLQQHT---------DLKPgDRIAVQLPNVLQYPVAVFGAMRAGLIVVntnP 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 134 LYKKHpvtELEYVIQDSQS-ALVIAA------EEYVGK-------ITPSAEKLGvpvlPLLRSCSDGSASH-----PAV- 193
Cdd:PRK05677 108 LYTAR---EMEHQFNDSGAkALVCLAnmahlaEKVLPKtgvkhviVTEVADMLP----PLKRLLINAVVKHvkkmvPAYh 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 194 --------------EEGPLTTCSSWKNRGAMIIYTSGTTGRPKGVLSTHEN-------VQAVTTGLVEKwewkKEDVILH 252
Cdd:PRK05677 181 lpqavkfndalakgAGQPVTEANPQADDVAVLQYTGGTTGVAKGAMLTHRNlvanmlqCRALMGSNLNE----GCEILIA 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 253 VLPLHHVHGVINKLLCPLWVGATCVMLP---EFNAqMVwkKLLSSQapRISVFMAVPTIYAKLVEyyDKHFfqpQVQDFV 329
Cdd:PRK05677 257 PLPLYHIYAFTFHCMAMMLIGNHNILISnprDLPA-MV--KELGKW--KFSGFVGLNTLFVALCN--NEAF---RKLDFS 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 330 RafcqenIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIG-MALSNPLHGVRIpGSVGTPLPGVEVCIatetlkdg 408
Cdd:PRK05677 327 A------LKLTLSGGMALQLATAERWKEVTGCAICEGYGMTETSpVVSVNPSQAIQV-GTIGIPVPSTLCKV-------- 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 409 arsytIHAQGDEsntqvTPglEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAYK-DGVYWIKGRTSvDI 487
Cdd:PRK05677 392 -----IDDDGNE-----LP--LGEVGELCVKGPQVMKGYWQRPEATDEILDSDGWLKTGDIALIQeDGYMRIVDRKK-DM 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 488 IKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEI 567
Cdd:PRK05677 459 ILVSGFNVYPNELEDVLAALPGVLQCAAIGVPDEKSGEAIKVFVVVKPGETLTKEQVMEHMRANLTGYKVPKAVEFRDEL 538
|
570
....*....|...
gi 2452540125 568 PRNQMGKVNKKEL 580
Cdd:PRK05677 539 PTTNVGKILRREL 551
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
107-580 |
1.64e-43 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 163.47 E-value: 1.64e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 107 ISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALVIAAEEYVGKITPsaekLGVPVL--PLLRSCS 184
Cdd:PLN02574 95 VLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGLAFTSPENVEKLSP----LGVPVIgvPENYDFD 170
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 185 DGSASHPAVEE------GPLTTCSSWKNRGAMIIYTSGTTGRPKGVLSTHENVQAvTTGLVEKWEWKK------EDVILH 252
Cdd:PLN02574 171 SKRIEFPKFYElikedfDFVPKPVIKQDDVAAIMYSSGTTGASKGVVLTHRNLIA-MVELFVRFEASQyeypgsDNVYLA 249
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 253 VLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWKKLlssQAPRISVFMAVPTIYAKLVeyydkHFFQPqvqdfVRAF 332
Cdd:PLN02574 250 ALPMFHIYGLSLFVVGLLSLGSTIVVMRRFDASDMVKVI---DRFKVTHFPVVPPILMALT-----KKAKG-----VCGE 316
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 333 CQENIRLMVSGSAALPVPVLEKWKSITGHT-LLERYGMTEigmalsnplhgvriPGSVGTPlpgvevCIATETLKD---- 407
Cdd:PLN02574 317 VLKSLKQVSCGAAPLSGKFIQDFVQTLPHVdFIQGYGMTE--------------STAVGTR------GFNTEKLSKyssv 376
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 408 GARSYTIHAQ-GDESNTQVTPglEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSv 485
Cdd:PLN02574 377 GLLAPNMQAKvVDWSTGCLLP--PGNCGELWIQGPGVMKGYLNNPKATQSTIDKDGWLRTGDIAYFdEDGYLYIVDRLK- 453
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 486 DIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVE 565
Cdd:PLN02574 454 EIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGSTLSQEAVINYVAKQVAPYKKVRKVVFVQ 533
|
490
....*....|....*
gi 2452540125 566 EIPRNQMGKVNKKEL 580
Cdd:PLN02574 534 SIPKSPAGKILRREL 548
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
209-580 |
1.03e-42 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 161.20 E-value: 1.03e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQA---------VTTGLVEKwewkKEDVILHVLPLHHVHGVINKLLCPLWVGATCVML 279
Cdd:PRK08751 211 AFLQYTGGTTGVAKGAMLTHRNLVAnmqqahqwlAGTGKLEE----GCEVVITALPLYHIFALTANGLVFMKIGGCNHLI 286
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 280 PEFNAQMVWKKLLssQAPRISVFMAVPTIYAKLVEY--YDKhffqpqvQDFvrafcqENIRLMVSGSAALPVPVLEKWKS 357
Cdd:PRK08751 287 SNPRDMPGFVKEL--KKTRFTAFTGVNTLFNGLLNTpgFDQ-------IDF------SSLKMTLGGGMAVQRSVAERWKQ 351
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 358 ITGHTLLERYGMTEIG-MALSNPLHGVRIPGSVGTPLPGVEVCIatetlKDgarsytihaqgDESNTQVTpgleGQEGEL 436
Cdd:PRK08751 352 VTGLTLVEAYGLTETSpAACINPLTLKEYNGSIGLPIPSTDACI-----KD-----------DAGTVLAI----GEIGEL 411
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 437 LVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAV 515
Cdd:PRK08751 412 CIKGPQVMKGYWKRPEETAKVMDADGWLHTGDIARMdEQGFVYIVDRKK-DMILVSGFNVYPNEIEDVIAMMPGVLEVAA 490
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2452540125 516 IGPPDMVWGQRVSAVVqLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK08751 491 VGVPDEKSGEIVKVVI-VKKDPALTAEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRREL 554
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
106-581 |
1.06e-42 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 159.47 E-value: 1.06e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 106 RISFLCPNDASYVVAQWASWmsggiavplykkhpvteleyVIQDSQSALVIAAEEYVGKITPSAEKLG-VPVLPLLRSCS 184
Cdd:cd05929 44 GVYIYLINSILTVFAAAAAW--------------------KCGACPAYKSSRAPRAEACAIIEIKAAAlVCGLFTGGGAL 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 185 DGSASHPAVEEGPLTTCSSWKNRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVE---KWEWKKEDVILHVLPLHHVHG 261
Cdd:cd05929 104 DGLEDYEAAEGGSPETPIEDEAAGWKMLYSGGTTGRPKGIKRGLPGGPPDNDTLMAaalGFGPGADSVYLSPAPLYHAAP 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 262 VINKLLCpLWVGATCVMLPEFNAQMVwkkLLSSQAPRISVFMAVPTIYAKLVEYYDKhffQPQVQDFvrafcqENIRLMV 341
Cdd:cd05929 184 FRWSMTA-LFMGGTLVLMEKFDPEEF---LRLIERYRVTFAQFVPTMFVRLLKLPEA---VRNAYDL------SSLKRVI 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 342 SGSAALPVPVLEKWKSITGHTLLERYGMTE-IGMALSNPLHGVRIPGSVGTPLPGvEVCIAtetlkdgarsytihaqgDE 420
Cdd:cd05929 251 HAAAPCPPWVKEQWIDWGGPIIWEYYGGTEgQGLTIINGEEWLTHPGSVGRAVLG-KVHIL-----------------DE 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 421 SNTQVTPGlegQEGELLVRGPSVFrEYWNRPRETEDAFTPDGWFKTGDTA-AYKDGVYWIKGRTSvDIIKNGGFKISALE 499
Cdd:cd05929 313 DGNEVPPG---EIGEVYFANGPGF-EYTNDPEKTAAARNEGGWSTLGDVGyLDEDGYLYLTDRRS-DMIISGGVNIYPQE 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 500 VERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSV---KDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVN 576
Cdd:cd05929 388 IENALIAHPKVLDAAVVGVPDEELGQRVHAVVQPAPGADAGTalaEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLY 467
|
....*
gi 2452540125 577 KKELL 581
Cdd:cd05929 468 RRLLR 472
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
59-580 |
1.13e-42 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 161.13 E-value: 1.13e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAII--DQNGEHTYRDLlsqslrlsQEICRALDCSSRDL---KEERISFLCPNDASYVVAQWASWMSGGIAV- 132
Cdd:PRK08315 26 AARYPDREALVyrDQGLRWTYREF--------NEEVDALAKGLLALgieKGDRVGIWAPNVPEWVLTQFATAKIGAILVt 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 133 --PLYKKHpvtELEYVIQDSQSALVIAAE-----EYVGKI--------TPSAEKLGVPVLPLLRS-CSDGSASHPaveeG 196
Cdd:PRK08315 98 inPAYRLS---ELEYALNQSGCKALIAADgfkdsDYVAMLyelapelaTCEPGQLQSARLPELRRvIFLGDEKHP----G 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 197 PLTTcSSWKNRGAM--------------------IIYTSGTTGRPKGVLSTHENVqaVTTGLV--EKWEWKKEDVILHVL 254
Cdd:PRK08315 171 MLNF-DELLALGRAvddaelaarqatldpddpinIQYTSGTTGFPKGATLTHRNI--LNNGYFigEAMKLTEEDRLCIPV 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 255 PLHHVHG-VINKLLCpLWVGATCV-MLPEFNAQMVwkkLLSSQAPRISVFMAVPTIY-AKLveyydKHffqPqvqDFVRa 331
Cdd:PRK08315 248 PLYHCFGmVLGNLAC-VTHGATMVyPGEGFDPLAT---LAAVEEERCTALYGVPTMFiAEL-----DH---P---DFAR- 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 332 FCQENIRLMVSGSAALPVPVLEKwksitghtLLERYGMTEI----GM----------ALSNPLHgVRIpGSVGTPLPGVE 397
Cdd:PRK08315 312 FDLSSLRTGIMAGSPCPIEVMKR--------VIDKMHMSEVtiayGMtetspvstqtRTDDPLE-KRV-TTVGRALPHLE 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 398 VCIAtetlkdgarsytihaqgDESNTQVTPglEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGV 476
Cdd:PRK08315 382 VKIV-----------------DPETGETVP--RGEQGELCTRGYSVMKGYWNDPEKTAEAIDADGWMHTGDLAVMdEEGY 442
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 477 YWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYT 556
Cdd:PRK08315 443 VNIVGRIK-DMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTEEDVRDFCRGKIAHYK 521
|
570 580
....*....|....*....|....
gi 2452540125 557 IPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK08315 522 IPRYIRFVDEFPMTVTGKIQKFKM 545
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
61-574 |
1.84e-42 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 160.05 E-value: 1.84e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 61 AFGDKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcSSRDLK-EERISFLCPNDASYVVAQWASWMSGGIAVPL---YK 136
Cdd:PRK07798 15 AVPDRVALVCGDRRLTYAELEERANRLA----HYL--IAQGLGpGDHVGIYARNRIEYVEAMLGAFKARAVPVNVnyrYV 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 137 KhpvTELEYVIQDSQSALVIAAEEYVGKItpsAEKLgvPVLPLLR---SCSDGSASHP---AVE-EGPLTTCSSWKNRGA 209
Cdd:PRK07798 89 E---DELRYLLDDSDAVALVYEREFAPRV---AEVL--PRLPKLRtlvVVEDGSGNDLlpgAVDyEDALAAGSPERDFGE 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 210 ------MIIYTSGTTGRPKGVLSTHENVQAVTTG---------LVEKWEWKKED------VILHVLPLHHVHGVINKLLC 268
Cdd:PRK07798 161 rspddlYLLYTGGTTGMPKGVMWRQEDIFRVLLGgrdfatgepIEDEEELAKRAaagpgmRRFPAPPLMHGAGQWAAFAA 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 269 pLWVGATCVMLP--EFNAQMVWKKLLSSQAPRISVF---MAVPtiyakLVEYYDKHffqpqvqdfvRAFCQENIRLMVSG 343
Cdd:PRK07798 241 -LFSGQTVVLLPdvRFDADEVWRTIEREKVNVITIVgdaMARP-----LLDALEAR----------GPYDLSSLFAIASG 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 344 SAALPVPVLEKW-KSITGHTLLERYGMTEIGmalsNPLHGVRIPGSVGTPLPGVEVciatetlkdGARSYTIhaqgDESN 422
Cdd:PRK07798 305 GALFSPSVKEALlELLPNVVLTDSIGSSETG----FGGSGTVAKGAVHTGGPRFTI---------GPRTVVL----DEDG 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 423 TQVTPGlEGQEGeLLVRGPSVFREYWNRPRETEDAF-TPDG--WFKTGDTAAY-KDGVYWIKGRTSVdIIKNGGFKISAL 498
Cdd:PRK07798 368 NPVEPG-SGEIG-WIARRGHIPLGYYKDPEKTAETFpTIDGvrYAIPGDRARVeADGTITLLGRGSV-CINTGGEKVFPE 444
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 499 EVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGK 574
Cdd:PRK07798 445 EVEEALKAHPDVADALVVGVPDERWGQEVVAVVQLREGARPDLAELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
59-581 |
2.04e-42 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 163.87 E-value: 2.04e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPLY 135
Cdd:COG1020 486 AARTPDAVAVVFGDQSLTYAELNARANRLA----HHL----RALgvgPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLD 557
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 136 KKHPVTELEYVIQDSQSALVIAAEEYVGKITPsaekLGVPVLPLlrscsDGSASHPAVEEGPLTTCSSwkNRGAMIIYTS 215
Cdd:COG1020 558 PAYPAERLAYMLEDAGARLVLTQSALAARLPE----LGVPVLAL-----DALALAAEPATNPPVPVTP--DDLAYVIYTS 626
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 216 GTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINkLLCPLWVGATCVMLPEFNAQMV--WKKLLS 293
Cdd:COG1020 627 GSTGRPKGVMVEHRALVNLLAWMQRRYGLGPGDRVLQFASLSFDASVWE-IFGALLSGATLVLAPPEARRDPaaLAELLA 705
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 294 SQapRISVFMAVPTIYAKLVEYydkhffQPQVQDFVRafcqeniRLMVSGSaALPVPVLEKWKSITGHT-LLERYGMTE- 371
Cdd:COG1020 706 RH--RVTVLNLTPSLLRALLDA------APEALPSLR-------LVLVGGE-ALPPELVRRWRARLPGArLVNLYGPTEt 769
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 372 -----IGMALSNPLHGVRIPgsVGTPLPGVEVCIATETLkdgarsytihaqgdesntQVTPglEGQEGELLVRGPSVFRE 446
Cdd:COG1020 770 tvdstYYEVTPPDADGGSVP--IGRPIANTRVYVLDAHL------------------QPVP--VGVPGELYIGGAGLARG 827
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 447 YWNRPRETEDAFTPDG-------WFKTGDTAAY-KDGVywIK--GRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVI 516
Cdd:COG1020 828 YLNRPELTAERFVADPfgfpgarLYRTGDLARWlPDGN--LEflGRAD-DQVKIRGFRIELGEIEAALLQHPGVREAVVV 904
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2452540125 517 GPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELL 581
Cdd:COG1020 905 AREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLPLPLTGNGKLDRLALP 969
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
128-583 |
6.31e-42 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 158.81 E-value: 6.31e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 128 GGIAVPLYKKHPVTELEYVIQDSQSALVIAAEEyvGKITPSAEKL--GVPVLPLLRSCsdgsasHPAVEEG--------- 196
Cdd:cd05970 96 GAIAIPATHQLTAKDIVYRIESADIKMIVAIAE--DNIPEEIEKAapECPSKPKLVWV------GDPVPEGwidfrklik 167
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 197 -------PLTTCSSWKNRGAMIIY-TSGTTGRPKGVlsTHENVQAVTTGLVEK-WEWKKEDVI-LHVLPLHHVHGVINKL 266
Cdd:cd05970 168 naspdfeRPTANSYPCGEDILLVYfSSGTTGMPKMV--EHDFTYPLGHIVTAKyWQNVREGGLhLTVADTGWGKAVWGKI 245
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 267 LCPlWVGATCVMLPEFNaQMVWKKLLSS-QAPRISVFMAVPTIYAKLVEYYDKHFfqpqvqDFvrafcqENIRLMVSGSA 345
Cdd:cd05970 246 YGQ-WIAGAAVFVYDYD-KFDPKALLEKlSKYGVTTFCAPPTIYRFLIREDLSRY------DL------SSLRYCTTAGE 311
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 346 ALPVPVLEKWKSITGHTLLERYGMTEIGMALSNpLHGVRI-PGSVGTPLPGVEVCIatetlkdgarsytIHAQGDESNTq 424
Cdd:cd05970 312 ALNPEVFNTFKEKTGIKLMEGFGQTETTLTIAT-FPWMEPkPGSMGKPAPGYEIDL-------------IDREGRSCEA- 376
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 425 vtpgleGQEGELLVR---GPSV--FREYWNRPRETEDAFTpDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISAL 498
Cdd:cd05970 377 ------GEEGEIVIRtskGKPVglFGGYYKDAEKTAEVWH-DGYYHTGDAAWMdEDGYLWFVGRTD-DLIKSSGYRIGPF 448
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 499 EVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLS---VKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKV 575
Cdd:cd05970 449 EVESALIQHPAVLECAVTGVPDPIRGQVVKATIVLAKGYEPSeelKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKI 528
|
....*...
gi 2452540125 576 NKKELLQR 583
Cdd:cd05970 529 RRVEIRER 536
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
48-580 |
6.36e-42 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 159.06 E-value: 6.36e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 48 YSSDVApVFTKALA-FGDKIAIIDQNGEHTYRDL----------LSQSLRLSqeicraldcssrdlKEERISFLCPNDAS 116
Cdd:PRK08974 22 YQSLVD-MFEQAVArYADQPAFINMGEVMTFRKLeersrafaayLQNGLGLK--------------KGDRVALMMPNLLQ 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 117 YVVAQWASWMSGGIAV---PLYKKHpvtELEYVIQDS-QSALVIAA------EEYVGK-------ITPSAEKLG------ 173
Cdd:PRK08974 87 YPIALFGILRAGMIVVnvnPLYTPR---ELEHQLNDSgAKAIVIVSnfahtlEKVVFKtpvkhviLTRMGDQLStakgtl 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 174 -----------VPVLPLLRSCSDGSASH---------PAVEEGPLttcsswknrgAMIIYTSGTTGRPKGVLSTHENVQA 233
Cdd:PRK08974 164 vnfvvkyikrlVPKYHLPDAISFRSALHkgrrmqyvkPELVPEDL----------AFLQYTGGTTGVAKGAMLTHRNMLA 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 234 VttglVEKWEW--------KKEDVILhVLPLHHVHGV-INKLLCpLWVGATCVM------LPEFNAQMvwKKLlssqapR 298
Cdd:PRK08974 234 N----LEQAKAaygpllhpGKELVVT-ALPLYHIFALtVNCLLF-IELGGQNLLitnprdIPGFVKEL--KKY------P 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 299 ISVFMAVPTIYAKLVEyyDKHFFQpqvQDFvrafcqENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMALS- 377
Cdd:PRK08974 300 FTAITGVNTLFNALLN--NEEFQE---LDF------SSLKLSVGGGMAVQQAVAERWVKLTGQYLLEGYGLTECSPLVSv 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 378 NPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPgleGQEGELLVRGPSVFREYWNRPRETEDA 457
Cdd:PRK08974 369 NPYDLDYYSGSIGLPVPSTEIKLV-----------------DDDGNEVPP---GEPGELWVKGPQVMLGYWQRPEATDEV 428
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 458 FTpDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVqLRKG 536
Cdd:PRK08974 429 IK-DGWLATGDIAVMdEEGFLRIVDRKK-DMILVSGFNVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFV-VKKD 505
|
570 580 590 600
....*....|....*....|....*....|....*....|....
gi 2452540125 537 EMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK08974 506 PSLTEEELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRREL 549
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
64-580 |
9.47e-42 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 156.37 E-value: 9.47e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEIcRALDCssrdLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:cd17649 2 DAVALVFGDQSLSYAELDARANRLAHRL-RALGV----GPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERL 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAAeeyvgkitpsaeklgvpvlpllrscsdgsasHPAveegplttcsswknRGAMIIYTSGTTGRPKG 223
Cdd:cd17649 77 RYMLEDSGAGLLLTH-------------------------------HPR--------------QLAYVIYTSGSTGTPKG 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 224 VLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLhHVHGVINKLLCPLWVGATCVMLP-------EFNAQMVwkkllssQA 296
Cdd:cd17649 112 VAVSHGPLAAHCQATAERYGLTPGDRELQFASF-NFDGAHEQLLPPLICGACVVLRPdelwasaDELAEMV-------RE 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 297 PRISVfMAVPTIYaklveyydkhfFQPQVQDFVRAFC--QENIRLMVSGSAALPVPVLEKWKSItGHTLLERYGMTE--- 371
Cdd:cd17649 184 LGVTV-LDLPPAY-----------LQQLAEEADRTGDgrPPSLRLYIFGGEALSPELLRRWLKA-PVRLFNAYGPTEatv 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 372 IGMALSNPLHGVRIPGSV--GTPLPGVEVCIATETLKdgarsytihaqgdesntqvtPGLEGQEGELLVRGPSVFREYWN 449
Cdd:cd17649 251 TPLVWKCEAGAARAGASMpiGRPLGGRSAYILDADLN--------------------PVPVGVTGELYIGGEGLARGYLG 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 450 RPRETEDAFTPDG-------WFKTGDTAAYK-DGVYWIKGRtsVD-IIKNGGFKISALEVERHLLAHPHITDVAVIGpPD 520
Cdd:cd17649 311 RPELTAERFVPDPfgapgsrLYRTGDLARWRdDGVIEYLGR--VDhQVKIRGFRIELGEIEAALLEHPGVREAAVVA-LD 387
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2452540125 521 MVWGQRVSAVVQLRKGEMLS--VKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17649 388 GAGGKQLVAYVVLRAAAAQPelRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKAL 449
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
105-583 |
2.47e-41 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 157.23 E-value: 2.47e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 105 ERISFLCPNDASYV-VAQWASWMsGGIAVPLYKKHPVTELEYVIQDSQSALVIAAEEYVgkitPSAEKLGVPVLPLLRSC 183
Cdd:PRK06155 72 DRVALMCGNRIEFLdVFLGCAWL-GAIAVPINTALRGPQLEHILRNSGARLLVVEAALL----AALEAADPGDLPLPAVW 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 184 SDGSASHPAVEEG-------PLTTCSSWKNRG----AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILH 252
Cdd:PRK06155 147 LLDAPASVSVPAGwstaplpPLDAPAPAAAVQpgdtAAILYTSGTTGPSKGVCCPHAQFYWWGRNSAEDLEIGADDVLYT 226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 253 VLPLHHVHGvINKLLCPLWVGATCVMLPEFNAQMVWKKLLSSQAPRISVFMAVPTIYAKlveyydkhffQPQVQDfvraf 332
Cdd:PRK06155 227 TLPLFHTNA-LNAFFQALLAGATYVLEPRFSASGFWPAVRRHGATVTYLLGAMVSILLS----------QPARES----- 290
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 333 cQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMALSNPlHGVRIPGSVGTPLPGVEVCIAtetlkdgarsy 412
Cdd:PRK06155 291 -DRAHRVRVALGPGVPAALHAAFRERFGVDLLDGYGSTETNFVIAVT-HGSQRPGSMGRLAPGFEARVV----------- 357
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 413 tihaqgDESNTQVTPGlegQEGELLVRG--PSVFRE-YWNRPRETEDAFTpDGWFKTGDTAAYK-DGVYWIKGRTSvDII 488
Cdd:PRK06155 358 ------DEHDQELPDG---EPGELLLRAdePFAFATgYFGMPEKTVEAWR-NLWFHTGDRVVRDaDGWFRFVDRIK-DAI 426
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 489 KNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIP 568
Cdd:PRK06155 427 RRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEDEVMAAVVLRDGTALEPVALVRHCEPRLAYFAVPRYVEFVAALP 506
|
490
....*....|....*
gi 2452540125 569 RNQMGKVNKKELLQR 583
Cdd:PRK06155 507 KTENGKVQKFVLREQ 521
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
74-517 |
3.53e-41 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 156.09 E-value: 3.53e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 74 EHTYRDLLSQSLRLSQEIcRALDCSSRDlkeeRISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSA 153
Cdd:cd05932 6 EFTWGEVADKARRLAAAL-RALGLEPGS----KIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESK 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 154 LVIaaeeyVGKITP-SAEKLGVP------------VLPLLRSCSDGSASHPAVEEGPLTTcsswKNRGAMIIYTSGTTGR 220
Cdd:cd05932 81 ALF-----VGKLDDwKAMAPGVPeglisislpppsAANCQYQWDDLIAQHPPLEERPTRF----PEQLATLIYTSGTTGQ 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 221 PKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATcVMLPE----FNAQMvwkkllssQA 296
Cdd:cd05932 152 PKGVMLTFGSFAWAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVL-VAFAEsldtFVEDV--------QR 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 297 PRISVFMAVPTIYAKlveyydkhfFQPQVQD--------------FVRAFCQENI---------RLMVSGSAALPVPVLE 353
Cdd:cd05932 223 ARPTLFFSVPRLWTK---------FQQGVQDkipqqklnlllkipVVNSLVKRKVlkglgldqcRLAGCGSAPVPPALLE 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 354 KWKSItGHTLLERYGMTEiGMALSN---PlhGVRIPGSVGTPLPGVEVCIatetlkdgarsytihaqgdesntqvtpgle 430
Cdd:cd05932 294 WYRSL-GLNILEAYGMTE-NFAYSHlnyP--GRDKIGTVGNAGPGVEVRI------------------------------ 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 431 GQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFK-ISALEVERHLLAHP 508
Cdd:cd05932 340 SEDGEILVRSPALMMGYYKDPEATAEAFTADGFLRTGDKGELdADGNLTITGRVK-DIFKTSKGKyVAPAPIENKLAEHD 418
|
....*....
gi 2452540125 509 HITDVAVIG 517
Cdd:cd05932 419 RVEMVCVIG 427
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
206-583 |
3.71e-41 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 156.88 E-value: 3.71e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 206 NRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGvINKLLCPLWVGATCVMLPEFNAQ 285
Cdd:PLN02860 172 DDAVLICFTSGTTGRPKGVTISHSALIVQSLAKIAIVGYGEDDVYLHTAPLCHIGG-LSSALAMLMVGACHVLLPKFDAK 250
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 286 MVWKKLlssQAPRISVFMAVPTIYAKLVEYYDKHffqpqvqdfVRAFCQENIRLMVSGSAALPVPVLEKWKSITGHT-LL 364
Cdd:PLN02860 251 AALQAI---KQHNVTSMITVPAMMADLISLTRKS---------MTWKVFPSVRKILNGGGSLSSRLLPDAKKLFPNAkLF 318
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 365 ERYGMTEIGMALS---------------------------NPLHGVripgSVGTPLPGVEVCIATetlkdgarsytihaq 417
Cdd:PLN02860 319 SAYGMTEACSSLTfmtlhdptlespkqtlqtvnqtksssvHQPQGV----CVGKPAPHVELKIGL--------------- 379
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 418 gDESNTqvtpglegqEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAYKD-GVYWIKGRTSvDIIKNGGFKIS 496
Cdd:PLN02860 380 -DESSR---------VGRILTRGPHVMLGYWGQNSETASVLSNDGWLDTGDIGWIDKaGNLWLIGRSN-DRIKTGGENVY 448
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 497 ALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLR-------------KGEM-LSVKDLKDWARD-TMAPYTIPTEL 561
Cdd:PLN02860 449 PEEVEAVLSQHPGVASVVVVGVPDSRLTEMVVACVRLRdgwiwsdnekenaKKNLtLSSETLRHHCREkNLSRFKIPKLF 528
|
410 420
....*....|....*....|...
gi 2452540125 562 IVVEE-IPRNQMGKVnKKELLQR 583
Cdd:PLN02860 529 VQWRKpFPLTTTGKI-RRDEVRR 550
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
74-582 |
4.39e-41 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 154.51 E-value: 4.39e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 74 EHTYRDLLSQSLRLSQEIcraldcSSRDL-KEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQS 152
Cdd:cd05971 6 KVTFKELKTASNRFANVL------KEIGLeKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 153 ALVIaaeeyvgkitpsaeklgvpvlpllrscSDGSaSHPAVeegplttcsswknrgamIIYTSGTTGRPKGVLSTHE--- 229
Cdd:cd05971 80 SALV---------------------------TDGS-DDPAL-----------------IIYTSGTTGPPKGALHAHRvll 114
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 230 ----NVQ-----AVTTGLV----EKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWkklLSSQA 296
Cdd:cd05971 115 ghlpGVQfpfnlFPRDGDLywtpADWAWIGGLLDVLLPSLYFGVPVLAHRMTKFDPKAALDLMSRYGVTTAF---LPPTA 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 297 PRISVFMAVPTiyaklveyydKHFfqpqvqdfvrafcQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMAL 376
Cdd:cd05971 192 LKMMRQQGEQL----------KHA-------------QVKLRAIATGGESLGEELLGWAREQFGVEVNEFYGQTECNLVI 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 377 SN--PLHGVRiPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPS--VFREYWNRPR 452
Cdd:cd05971 249 GNcsALFPIK-PGSMGKPIPGHRVAIV-----------------DDNGTPLPPG---EVGEIAVELPDpvAFLGYWNNPS 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 453 ETEDAFTPDgWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVV 531
Cdd:cd05971 308 ATEKKMAGD-WLLTGDLGRKdSDGYFWYVGRDD-DVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFV 385
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 2452540125 532 QLRKGEMLS---VKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQ 582
Cdd:cd05971 386 VLNPGETPSdalAREIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELRA 439
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
63-580 |
4.59e-41 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 155.13 E-value: 4.59e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 63 GDKIAIIDQNGEHTYRDLLSQSLRLSQEIcRALDCSSrdlkEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTE 142
Cdd:cd17646 12 PDAPAVVDEGRTLTYRELDERANRLAHLL-RARGVGP----EDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPGYPADR 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 143 LEYVIQDSQSALVIAAEEYVGKITPsaekLGVPVLPLLRSCSDGSASHPAVEEGPlttcsswkNRGAMIIYTSGTTGRPK 222
Cdd:cd17646 87 LAYMLADAGPAVVLTTADLAARLPA----GGDVALLGDEALAAPPATPPLVPPRP--------DNLAYVIYTSGSTGRPK 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 223 GVLSTHEnvqavttGLVEKWEWKKE-------DVILHVLPLhhvhGV---INKLLCPLWVGATCVML-------PEFNAQ 285
Cdd:cd17646 155 GVMVTHA-------GIVNRLLWMQDeyplgpgDRVLQKTPL----SFdvsVWELFWPLVAGARLVVArpgghrdPAYLAA 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 286 MVwkkllssQAPRISVFMAVPTIYAKLVEyydkhffQPQVQDfvrafCQeNIRLMVSGSAALPVPVLEKWKSITGHTLLE 365
Cdd:cd17646 224 LI-------REHGVTTCHFVPSMLRVFLA-------EPAAGS-----CA-SLRRVFCSGEALPPELAARFLALPGAELHN 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 366 RYGMTE--IGM---ALSNPLHGVRIPgsVGTPLPGVEVCIATETLkdgarsytihaqgdesntQVTPglEGQEGELLVRG 440
Cdd:cd17646 284 LYGPTEaaIDVthwPVRGPAETPSVP--IGRPVPNTRLYVLDDAL------------------RPVP--VGVPGELYLGG 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 441 PSVFREYWNRPRETEDAFTPDgWF-------KTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITD 512
Cdd:cd17646 342 VQLARGYLGRPALTAERFVPD-PFgpgsrmyRTGDLARWRpDGALEFLGRSD-DQVKIRGFRVEPGEIEAALAAHPAVTH 419
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2452540125 513 VAVIGPPDMVWGQRVSAVVQLRKGEM-LSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17646 420 AVVVARAAPAGAARLVGYVVPAAGAAgPDTAALRAHLAERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
209-577 |
1.19e-40 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 150.87 E-value: 1.19e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLV-EKWEWKKEDVILHVLPLHHVHGVINKLLCpLWVGATCVMLPEFNAQMV 287
Cdd:cd17635 4 LAVIFTSGTTGEPKAVLLANKTFFAVPDILQkEGLNWVVGDVTYLPLPATHIGGLWWILTC-LIHGGLCVTGGENTTYKS 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 288 WKKLLSSQAPRISVFmaVPTIYAKLVEYYDKhffqpqvqdfVRAFCqENIRLMVSGSAaLPVPVLEKWKSITGHT-LLER 366
Cdd:cd17635 83 LFKILTTNAVTTTCL--VPTLLSKLVSELKS----------ANATV-PSLRLIGYGGS-RAIAADVRFIEATGLTnTAQV 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 367 YGMTEIGMALSNPLH-GVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgdesNTQVTPGLEGQEGELLVRGPSVFR 445
Cdd:cd17635 149 YGLSETGTALCLPTDdDSIEINAVGRPYPGVDVYLA--------------------ATDGIAGPSASFGTIWIKSPANML 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 446 EYWNRPRETEDAFTpDGWFKTGDTA-AYKDGVYWIKGRTSVDIIKnGGFKISALEVERHLLAHPHITDVAVIGPPDMVWG 524
Cdd:cd17635 209 GYWNNPERTAEVLI-DGWVNTGDLGeRREDGFLFITGRSSESINC-GGVKIAPDEVERIAEGVSGVQECACYEISDEEFG 286
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 525 QRVSAVVQlrKGEML---SVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNK 577
Cdd:cd17635 287 ELVGLAVV--ASAELdenAIRALKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
47-580 |
1.34e-40 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 155.18 E-value: 1.34e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 47 AYSSdVAPVFTKALA-FGDKIAIIDQNGEHTYRDLLSQSLRLSQEIcraldcSSRDL-KEERISFLCPNDASYVVAQWAS 124
Cdd:PRK07059 21 QYPS-LADLLEESFRqYADRPAFICMGKAITYGELDELSRALAAWL------QSRGLaKGARVAIMMPNVLQYPVAIAAV 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 125 WMSGGIAV---PLYKKHpvtELEYVIQDSQS-ALVI------AAEEYVGK-------ITPSAEKLG-------------- 173
Cdd:PRK07059 94 LRAGYVVVnvnPLYTPR---ELEHQLKDSGAeAIVVlenfatTVQQVLAKtavkhvvVASMGDLLGfkghivnfvvrrvk 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 174 --VPV--LPLLRSCSDgsashpAVEEG---PLTTCSSWKNRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLvEKW---- 242
Cdd:PRK07059 171 kmVPAwsLPGHVRFND------ALAEGarqTFKPVKLGPDDVAFLQYTGGTTGVSKGATLLHRNIVANVLQM-EAWlqpa 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 243 -EWKKED---VILHVLPLHHVHGVINKLLCPLWVGATCVMLPefN----AQMVwKKLLSSQaprISVFMAVPTIYAKLVE 314
Cdd:PRK07059 244 fEKKPRPdqlNFVCALPLYHIFALTVCGLLGMRTGGRNILIP--NprdiPGFI-KELKKYQ---VHIFPAVNTLYNALLN 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 315 YYDkhfFQpQVqDFvrafcqENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIG-MALSNPLHGVRIPGSVGTPL 393
Cdd:PRK07059 318 NPD---FD-KL-DF------SKLIVANGGGMAVQRPVAERWLEMTGCPITEGYGLSETSpVATCNPVDATEFSGTIGLPL 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 394 PGVEVCIatetlKDgarsytihaqgDESNTQVTpgleGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTaayk 473
Cdd:PRK07059 387 PSTEVSI-----RD-----------DDGNDLPL----GEPGEICIRGPQVMAGYWNRPDETAKVMTADGFFRTGDV---- 442
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 474 dGVYWIKGRTSV-----DIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVqLRKGEMLSVKDLKDWA 548
Cdd:PRK07059 443 -GVMDERGYTKIvdrkkDMILVSGFNVYPNEIEEVVASHPGVLEVAAVGVPDEHSGEAVKLFV-VKKDPALTEEDVKAFC 520
|
570 580 590
....*....|....*....|....*....|..
gi 2452540125 549 RDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK07059 521 KERLTNYKRPKFVEFRTELPKTNVGKILRREL 552
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
55-580 |
3.50e-40 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 153.60 E-value: 3.50e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 55 VFTKALAFGDKIAIID-QNGE-HTYRDLlsqslrlsQEICRALDCSSRDL---KEERISFLCPNDASYVVAQWASWMSGG 129
Cdd:PLN02246 29 CFERLSEFSDRPCLIDgATGRvYTYADV--------ELLSRRVAAGLHKLgirQGDVVMLLLPNCPEFVLAFLGASRRGA 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 130 I---AVPLYKKHpvtELEYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLpLLRSCSDGSASHPAV---EEGPLTTCSS 203
Cdd:PLN02246 101 VtttANPFYTPA---EIAKQAKASGAKLIITQSCYVDKLKGLAEDDGVTVV-TIDDPPEGCLHFSELtqaDENELPEVEI 176
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 204 WKNRGAMIIYTSGTTGRPKGVLSTHENVqaVTT------GLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCV 277
Cdd:PLN02246 177 SPDDVVALPYSSGTTGLPKGVMLTHKGL--VTSvaqqvdGENPNLYFHSDDVILCVLPMFHIYSLNSVLLCGLRVGAAIL 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 278 MLPEFNAqmvwKKLLSS-QAPRISVFMAVPTI---YAK--LVEYYDkhffqpqvqdfvrafcQENIRLMVSGSAALPVPV 351
Cdd:PLN02246 255 IMPKFEI----GALLELiQRHKVTIAPFVPPIvlaIAKspVVEKYD----------------LSSIRMVLSGAAPLGKEL 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 352 LEKWKS-ITGHTLLERYGMTEIGMALSNPLHGVRIP-----GSVGTPLPGVEVCIA-TETLKDGARsytihaqgdesntq 424
Cdd:PLN02246 315 EDAFRAkLPNAVLGQGYGMTEAGPVLAMCLAFAKEPfpvksGSCGTVVRNAELKIVdPETGASLPR-------------- 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 425 vtpgleGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAaYKDG------VYWIKgrtsvDIIKNGGFKISAL 498
Cdd:PLN02246 381 ------NQPGEICIRGPQIMKGYLNDPEATANTIDKDGWLHTGDIG-YIDDddelfiVDRLK-----ELIKYKGFQVAPA 448
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 499 EVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKK 578
Cdd:PLN02246 449 ELEALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGSEITEDEIKQFVAKQVVFYKRIHKVFFVDSIPKAPSGKILRK 528
|
..
gi 2452540125 579 EL 580
Cdd:PLN02246 529 DL 530
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
59-580 |
7.31e-40 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 151.97 E-value: 7.31e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPLY 135
Cdd:cd12117 7 AARTPDAVAVVYGDRSLTYAELNERANRLA----RRL----RAAgvgPGDVVGVLAERSPELVVALLAVLKAGAAYVPLD 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 136 KKHPVTELEYVIQDSQSALVIAAEEYvgkiTPSAEKLGVPVLPLLrscsdgsASHPAVEEGPLTTCSSwkNRGAMIIYTS 215
Cdd:cd12117 79 PELPAERLAFMLADAGAKVLLTDRSL----AGRAGGLEVAVVIDE-------ALDAGPAGNPAVPVSP--DDLAYVMYTS 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 216 GTTGRPKGVLSTHENVqavtTGLVEKWEW---KKEDVILHVLPL------HHVHGvinkllcPLWVGATCVMLP--EFNA 284
Cdd:cd12117 146 GSTGRPKGVAVTHRGV----VRLVKNTNYvtlGPDDRVLQTSPLafdastFEIWG-------ALLNGARLVLAPkgTLLD 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 285 QMVWKKLLSSQapRISV-FMAVPtiyaklveyydkhFFQPQVQDFVRAFcqENIRLMVSGSAALPVPVLEKWKSITGH-T 362
Cdd:cd12117 215 PDALGALIAEE--GVTVlWLTAA-------------LFNQLADEDPECF--AGLRELLTGGEVVSPPHVRRVLAACPGlR 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 363 LLERYGMTE-IGMALSNPL-HGVRIPGSV--GTPLPGVEVCIATETLkdgarsytihaqgdesntQVTPglEGQEGELLV 438
Cdd:cd12117 278 LVNGYGPTEnTTFTTSHVVtELDEVAGSIpiGRPIANTRVYVLDEDG------------------RPVP--PGVPGELYV 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 439 RGPSVFREYWNRPRETEDAFTPDGW------FKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHIT 511
Cdd:cd12117 338 GGDGLALGYLNRPALTAERFVADPFgpgerlYRTGDLARWLpDGRLEFLGRID-DQVKIRGFRIELGEIEAALRAHPGVR 416
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 512 DVAVI-GPPDMVWGQRVSAVVqlrKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd12117 417 EAVVVvREDAGGDKRLVAYVV---AEGALDAAELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
64-580 |
7.47e-40 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 151.86 E-value: 7.47e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLsqslRLSQEICRALdcSSRDLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:PRK07638 16 NKIAIKENDRVLTYKDWF----ESVCKVANWL--NEKESKNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDEL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAAEEYVGKITpSAEklgVPVLpLLRSCSdgsashPAVEEGpLTTCSSWKNRGAMIIY---TSGTTGR 220
Cdd:PRK07638 90 KERLAISNADMIVTERYKLNDLP-DEE---GRVI-EIDEWK------RMIEKY-LPTYAPIENVQNAPFYmgfTSGSTGK 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 221 PKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVH---GVINKLlcplWVGATCVMLPEFNAQMVWKKLLSSQap 297
Cdd:PRK07638 158 PKAFLRAQQSWLHSFDCNVHDFHMKREDSVLIAGTLVHSLflyGAISTL----YVGQTVHLMRKFIPNQVLDKLETEN-- 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 298 rISVFMAVPTIYAKLVEyydkhffqpqvqdfVRAFCQENIRLMVSGsAALPVPVLEKWKSITGH-TLLERYGMTEIG-MA 375
Cdd:PRK07638 232 -ISVMYTVPTMLESLYK--------------ENRVIENKMKIISSG-AKWEAEAKEKIKNIFPYaKLYEFYGASELSfVT 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 376 LSNPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFREYWNRPRETE 455
Cdd:PRK07638 296 ALVDEESERRPNSVGRPFHNVQVRIC-----------------NEAGEEVQKG---EIGTVYVKSPQFFMGYIIGGVLAR 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 456 DaFTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVqlr 534
Cdd:PRK07638 356 E-LNADGWMTVRDVGYEdEEGFIYIVGREK-NMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPVAII--- 430
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 2452540125 535 KGEMlSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK07638 431 KGSA-TKQQLKSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEA 475
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
210-575 |
8.57e-40 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 147.94 E-value: 8.57e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 210 MIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGvINKLLCPLWVGATCVMLPEFNAQMVWK 289
Cdd:cd17633 4 YIGFTSGTTGLPKAYYRSERSWIESFVCNEDLFNISGEDAILAPGPLSHSLF-LYGAISALYLGGTFIGQRKFNPKSWIR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 290 KLlssQAPRISVFMAVPTIYAKLVEYYDKHffqpqvqdfvrafcqENIRLMVSGSAALPVPVLEKWKSITGHT-LLERYG 368
Cdd:cd17633 83 KI---NQYNATVIYLVPTMLQALARTLEPE---------------SKIKSIFSSGQKLFESTKKKLKNIFPKAnLIEFYG 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 369 MTEIGMALSNPLHGVRIPGSVGTPLPGVEVciatetlkdgarsYTIHAQGDEsntqvtpglegqEGELLVRGPSVFREYW 448
Cdd:cd17633 145 TSELSFITYNFNQESRPPNSVGRPFPNVEI-------------EIRNADGGE------------IGKIFVKSEMVFSGYV 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 449 nrpreTEDAFTPDGWFKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRV 527
Cdd:cd17633 200 -----RGGFSNPDGWMSVGDIGYVDeEGYLYLVGRES-DMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEIA 273
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 2452540125 528 SAVVqlrKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKV 575
Cdd:cd17633 274 VALY---SGDKLTYKQLKRFLKQKLSRYEIPKKIIFVDSLPYTSSGKI 318
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
200-580 |
9.06e-40 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 152.62 E-value: 9.06e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 200 TCSSWKNRGAMIIY-TSGTTGRPKGVLSTHENVQAVTTGLVEKW-EWKKEDVILHVLPLHHVHGVINKLLCPlWVGATCV 277
Cdd:cd05928 167 HCVETGSQEPMAIYfTSGTTGSPKMAEHSHSSLGLGLKVNGRYWlDLTASDIMWNTSDTGWIKSAWSSLFEP-WIQGACV 245
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 278 M---LPEFNAQMVWKKLlsSQAPrISVFMAVPTIYAKLVEyydkhffqpqvQDFVRaFCQENIRLMVSGSAALPVPVLEK 354
Cdd:cd05928 246 FvhhLPRFDPLVILKTL--SSYP-ITTFCGAPTVYRMLVQ-----------QDLSS-YKFPSLQHCVTGGEPLNPEVLEK 310
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 355 WKSITGHTLLERYGMTEIGMALSNPlHGVRI-PGSVGTPLPGVEVCIATEtlkdgarsytihaqgdesNTQVTPglEGQE 433
Cdd:cd05928 311 WKAQTGLDIYEGYGQTETGLICANF-KGMKIkPGSMGKASPPYDVQIIDD------------------NGNVLP--PGTE 369
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 434 GELLVR-GP----SVFREYWNRPRETEDAFTPDGWFkTGDTAAY-KDGVYWIKGRtSVDIIKNGGFKISALEVERHLLAH 507
Cdd:cd05928 370 GDIGIRvKPirpfGLFSGYVDNPEKTAATIRGDFYL-TGDRGIMdEDGYFWFMGR-ADDVINSSGYRIGPFEVESALIEH 447
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2452540125 508 PHITDVAVIGPPDMVWGQRVSAVVQL------RKGEMLsVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05928 448 PAVVESAVVSSPDPIRGEVVKAFVVLapqflsHDPEQL-TKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNEL 525
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
62-582 |
1.08e-39 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 152.30 E-value: 1.08e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 62 FGDKIAIID-QNG-EHTYRDLLSQSLRLSqEICRALDCSSRDlkeeRISFLCPNDASYVVAQWASWMSGGIAVPLYKKHP 139
Cdd:cd17642 30 VPGTIAFTDaHTGvNYSYAEYLEMSVRLA-EALKKYGLKQND----RIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYN 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 140 VTELEYVIQDSQSALVIAAEEYVGKITPSAEKLG-VPVLPLLRSCSD-----------GSASHPAVEEGPLTTCSSWKNR 207
Cdd:cd17642 105 ERELDHSLNISKPTIVFCSKKGLQKVLNVQKKLKiIKTIIILDSKEDykgyqclytfiTQNLPPGFNEYDFKPPSFDRDE 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 208 G-AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEK---WEWKKEDVILHVLPLHHVHGVINkLLCPLWVGATCVMLPEFN 283
Cdd:cd17642 185 QvALIMNSSGSTGLPKGVQLTHKNIVARFSHARDPifgNQIIPDTAILTVIPFHHGFGMFT-TLGYLICGFRVVLMYKFE 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 284 AQMVWKKLlssQAPRISVFMAVPTIYA-----KLVEYYDkhffqpqvqdfvrafcQENIRLMVSGSAALPVPVLEKWKSI 358
Cdd:cd17642 264 EELFLRSL---QDYKVQSALLVPTLFAffaksTLVDKYD----------------LSNLHEIASGGAPLSKEVGEAVAKR 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 359 TGHTLLER-YGMTEIGMALSNPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgdESNTQVTPGLEgQEGELL 437
Cdd:cd17642 325 FKLPGIRQgYGLTETTSAILITPEGDDKPGAVGKVVPFFYAKVV------------------DLDTGKTLGPN-ERGELC 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 438 VRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVI 516
Cdd:cd17642 386 VKGPMIMKGYVNNPEATKALIDKDGWLHSGDIAYYdEDGHFFIVDRLK-SLIKYKGYQVPPAELESILLQHPKIFDAGVA 464
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 517 GPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYT-IPTELIVVEEIPRNQMGKVNKKELLQ 582
Cdd:cd17642 465 GIPDEDAGELPAAVVVLEAGKTMTEKEVMDYVASQVSTAKrLRGGVKFVDEVPKGLTGKIDRRKIRE 531
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
61-575 |
2.01e-39 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 151.97 E-value: 2.01e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 61 AFGDKIAI--IDQNGEH--TYRDLLSQSLRLsqeiCRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVP 133
Cdd:PRK04319 56 GRKDKVALryLDASRKEkyTYKELKELSNKF----ANVL----KELgveKGDRVFIFMPRIPELYFALLGALKNGAIVGP 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 134 LYK---KHPVTELeyvIQDSQSALVIAaeeyvgkiTPSA-EKLGVPVLPLLRSC----SDGSAS------HPAVEEGPLT 199
Cdd:PRK04319 128 LFEafmEEAVRDR---LEDSEAKVLIT--------TPALlERKPADDLPSLKHVllvgEDVEEGpgtldfNALMEQASDE 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 200 TCSSWKNR--GAMIIYTSGTTGRPKGVLSTHENV-QAVTTGlveKWewkkedvilhVLPLHH----------------VH 260
Cdd:PRK04319 197 FDIEWTDRedGAILHYTSGSTGKPKGVLHVHNAMlQHYQTG---KY----------VLDLHEddvywctadpgwvtgtSY 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 261 GVINKLLCplwvGATCVML-PEFNAQMvWKKLLSSQapRISVFMAVPTIYAKLVEYYDkhffqpqvqDFVRAFCQENIRL 339
Cdd:PRK04319 264 GIFAPWLN----GATNVIDgGRFSPER-WYRILEDY--KVTVWYTAPTAIRMLMGAGD---------DLVKKYDLSSLRH 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 340 MVS-GSAALPVPVleKW-KSITGHTLLERYGMTEIG--MALSNPLHGVRiPGSVGTPLPGVEVCIatetlkdgarsytIH 415
Cdd:PRK04319 328 ILSvGEPLNPEVV--RWgMKVFGLPIHDNWWMTETGgiMIANYPAMDIK-PGSMGKPLPGIEAAI-------------VD 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 416 AQGDEsntqVTPGlegQEGELLVRG--PSVFREYWNRPRETEDAFTpDGWFKTGDTAaYKD--GVYWIKGRTSvDIIKNG 491
Cdd:PRK04319 392 DQGNE----LPPN---RMGNLAIKKgwPSMMRGIWNNPEKYESYFA-GDWYVSGDSA-YMDedGYFWFQGRVD-DVIKTS 461
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 492 GFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKG----EMLSvKDLKDWARDTMAPYTIPTELIVVEEI 567
Cdd:PRK04319 462 GERVGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGyepsEELK-EEIRGFVKKGLGAHAAPREIEFKDKL 540
|
....*...
gi 2452540125 568 PRNQMGKV 575
Cdd:PRK04319 541 PKTRSGKI 548
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
76-517 |
2.08e-39 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 150.20 E-value: 2.08e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSqeicraldCSSRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQS 152
Cdd:cd17640 7 TYKDLYQEILDFA--------AGLRSLgvkAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSES 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 153 ALVIaaeeyvgkitpsaeklgvpvlpllrscsdgsashpaVEEGPlttcsswkNRGAMIIYTSGTTGRPKGVLSTHENVq 232
Cdd:cd17640 79 VALV------------------------------------VENDS--------DDLATIIYTSGTTGNPKGVMLTHANL- 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 233 avTTGLVEKWEW---KKEDVILHVLPLHHVHGVINKLLCPLWvGATCVmlpeFNAQMVWKKLLSSQAPriSVFMAVPTIY 309
Cdd:cd17640 114 --LHQIRSLSDIvppQPGDRFLSILPIWHSYERSAEYFIFAC-GCSQA----YTSIRTLKDDLKRVKP--HYIVSVPRLW 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 310 AKLVE-YYDKHFFQPQVQDFVRAFCQ--ENIRLMVSGSAALPvPVLEKWKSITGHTLLERYGMTEIGMALSNPLHGVRIP 386
Cdd:cd17640 185 ESLYSgIQKQVSKSSPIKQFLFLFFLsgGIFKFGISGGGALP-PHVDTFFEAIGIEVLNGYGLTETSPVVSARRLKCNVR 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 387 GSVGTPLPGVEVCIAtetlkdgarsytihaqGDESNTQVTPGlegQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKT 466
Cdd:cd17640 264 GSVGRPLPGTEIKIV----------------DPEGNVVLPPG---EKGIVWVRGPQVMKGYYKNPEATSKVLDSDGWFNT 324
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|..
gi 2452540125 467 GDTAAY-KDGVYWIKGRTSVDIIKNGGFKISALEVERHLLAHPHITDVAVIG 517
Cdd:cd17640 325 GDLGWLtCGGELVLTGRAKDTIVLSNGENVEPQPIEEALMRSPFIEQIMVVG 376
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
54-580 |
5.62e-39 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 150.15 E-value: 5.62e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 54 PVFTKALAFGDKIAIIDQNGEHTYRDLLSQSLRLSQEICRALDCSSRdlkeeRISFLCPNDASYVVAQWASWMSGGIAVP 133
Cdd:PRK13383 40 LLAVTAARWPGRTAIIDDDGALSYRELQRATESLARRLTRDGVAPGR-----AVGVMCRNGRGFVTAVFAVGLLGADVVP 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 134 LYKKHPVTELEYVIQDSQSALVIAAEEYVGKITPSAEKLGVpVLPLLRSCSDgSASHPAVEEGplttcsswknrGAMIIY 213
Cdd:PRK13383 115 ISTEFRSDALAAALRAHHISTVVADNEFAERIAGADDAVAV-IDPATAGAEE-SGGRPAVAAP-----------GRIVLL 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 214 TSGTTGRPKGVLSTHENVQA--VTTGLVEKWEWKKEDVILHVLPLHHVHGvINKLLCPLWVGATCVMLPEFNAQMVwkkL 291
Cdd:PRK13383 182 TSGTTGKPKGVPRAPQLRSAvgVWVTILDRTRLRTGSRISVAMPMFHGLG-LGMLMLTIALGGTVLTHRHFDAEAA---L 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 292 LSSQAPRISVFMAVPTIYAKLVEyydkhfFQPQVQDFVRAFCqenIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTE 371
Cdd:PRK13383 258 AQASLHRADAFTAVPVVLARILE------LPPRVRARNPLPQ---LRVVMSSGDRLDPTLGQRFMDTYGDILYNGYGSTE 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 372 IGM-ALSNPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGLEGQ---EGELlvrgpsvfrey 447
Cdd:PRK13383 329 VGIgALATPADLRDAPETVGKPVAGCPVRIL-----------------DRNNRPVGPRVTGRifvGGEL----------- 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 448 wNRPRETEDAFTP--DGWFKTGDTaAYKD--GVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVW 523
Cdd:PRK13383 381 -AGTRYTDGGGKAvvDGMTSTGDM-GYLDnaGRLFIVGRED-DMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERF 457
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 524 GQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK13383 458 GHRLAAFVVLHPGSGVDAAQLRDYLKDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
70-583 |
9.98e-39 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 148.63 E-value: 9.98e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 70 DQNG-EHTYRDLLSQSLRLSQEIcraldcSSRDLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQ 148
Cdd:cd05909 2 DTLGtSLTYRKLLTGAIALARKL------AKMTKEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIK 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 149 DSQSALVIAAEEYVGKIT-----------------------PSAEKLGV------PVLPLLRS--CSDGSASHPAVeegp 197
Cdd:cd05909 76 LAGIKTVLTSKQFIEKLKlhhlfdveydarivyledlrakiSKADKCKAflagkfPPKWLLRIfgVAPVQPDDPAV---- 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 198 lttcsswknrgamIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCV 277
Cdd:cd05909 152 -------------ILFTSGSEGLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKVV 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 278 MLP-----EFNAQMVWKKllssqapRISVFMAVPT---IYAKLVEYYDkhFFqpqvqdfvrafcqeNIRLMVSGSAALPV 349
Cdd:cd05909 219 FHPnpldyKKIPELIYDK-------KATILLGTPTflrGYARAAHPED--FS--------------SLRLVVAGAEKLKD 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 350 PVLEKWKSITGHTLLERYGMTEIG--MALSNPLHGVRiPGSVGTPLPGVEVCIatetlkdgarsytIHAQGDEsntqvtP 427
Cdd:cd05909 276 TLRQEFQEKFGIRILEGYGTTECSpvISVNTPQSPNK-EGTVGRPLPGMEVKI-------------VSVETHE------E 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 428 GLEGQEGELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLA 506
Cdd:cd05909 336 VPIGEGGLLLVRGPNVMLGYLNEPELTSFAFG-DGWYDTGDIGKIdGEGFLTITGRLS-RFAKIAGEMVSLEAIEDILSE 413
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2452540125 507 H-PHITDVAVIGPPDMVWGQRVSAVVQlrkGEMLSVKDLKDWARDTMAP-YTIPTELIVVEEIPRNQMGKVNKKELLQR 583
Cdd:cd05909 414 IlPEDNEVAVVSVPDGRKGEKIVLLTT---TTDTDPSSLNDILKNAGISnLAKPSYIHQVEEIPLLGTGKPDYVTLKAL 489
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
76-584 |
3.71e-38 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 147.81 E-value: 3.71e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSQEIcRALDCSSRDlkeeRISFLCPNDASYVVAQWASWMSGGIAVPL-----YKK--HPVTELEYVIQ 148
Cdd:cd05906 41 SYQDLLEDARRLAAGL-RQLGLRPGD----SVILQFDDNEDFIPAFWACVLAGFVPAPLtvpptYDEpnARLRKLRHIWQ 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 149 DSQSALVIAAEEYVGKITPSAEKLGVPVLPLL--RSCSDGSASHPAVEEGPLTTcsswknrgAMIIYTSGTTGRPKGVLS 226
Cdd:cd05906 116 LLGSPVVLTDAELVAEFAGLETLSGLPGIRVLsiEELLDTAADHDLPQSRPDDL--------ALLMLTSGSTGFPKAVPL 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 227 THENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLP--EFNAQ-MVWKKLLssQAPRISVFM 303
Cdd:cd05906 188 THRNILARSAGKIQHNGLTPQDVFLNWVPLDHVGGLVELHLRAVYLGCQQVHVPteEILADpLRWLDLI--DRYRVTITW 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 304 AVPTIYAKLVEYYDKhfFQPQVQDFvrafcqENIRLMVSGSAALPVPVLEKWKSitghtLLERY-----------GMTEI 372
Cdd:cd05906 266 APNFAFALLNDLLEE--IEDGTWDL------SSLRYLVNAGEAVVAKTIRRLLR-----LLEPYglppdairpafGMTET 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 373 --GMALSNPLHGVRIPG-----SVGTPLPGVEVCIAtetlkdgarsytihaqgDESNtQVTPglEGQEGELLVRGPSVFR 445
Cdd:cd05906 333 csGVIYSRSFPTYDHSQalefvSLGRPIPGVSMRIV-----------------DDEG-QLLP--EGEVGRLQVRGPVVTK 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 446 EYWNRPRETEDAFTPDGWFKTGDTAAYKDGVYWIKGRTSVDIIKNgGFKISALEVERHL-----------LAHPH----- 509
Cdd:cd05906 393 GYYNNPEANAEAFTEDGWFRTGDLGFLDNGNLTITGRTKDTIIVN-GVNYYSHEIEAAVeevpgvepsftAAFAVrdpga 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 510 ITDVAVI------GPPDmvwgQRVSAVVQLRKGEMLSVkdlkdwardTMAP-YTIPTELivvEEIPRNQMGKVNKKELLQ 582
Cdd:cd05906 472 ETEELAIffvpeyDLQD----ALSETLRAIRSVVSREV---------GVSPaYLIPLPK---EEIPKTSLGKIQRSKLKA 535
|
..
gi 2452540125 583 RF 584
Cdd:cd05906 536 AF 537
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
64-580 |
7.34e-38 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 146.33 E-value: 7.34e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEIcRALDCSSRDlkeeRISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:cd17651 10 DAPALVAEGRRLTYAELDRRANRLAHRL-RARGVGPGD----LVALCARRSAELVVALLAILKAGAAYVPLDPAYPAERL 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLPLLRSCSDGSashPAVEEGPlttcsswkNRGAMIIYTSGTTGRPKG 223
Cdd:cd17651 85 AFMLADAGPVLVLTHPALAGELAVELVAVTLLDQPGAAAGADAE---PDPALDA--------DDLAYVIYTSGSTGRPKG 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 224 VLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLH---HVHGVINKLLCplwvGATCVMLPEfnaqmvwkkllssqapriS 300
Cdd:cd17651 154 VVMPHRSLANLVAWQARASSLGPGARTLQFAGLGfdvSVQEIFSTLCA----GATLVLPPE------------------E 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 301 VFMAVPTIYAKLVEYYDKHFFQPQVqdFVRAFCQENIR----------LMVSGSAALPVPVLEKW-KSITGHTLLERYGM 369
Cdd:cd17651 212 VRTDPPALAAWLDEQRISRVFLPTV--ALRALAEHGRPlgvrlaalryLLTGGEQLVLTEDLREFcAGLPGLRLHNHYGP 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 370 TE----IGMALSNPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFR 445
Cdd:cd17651 290 TEthvvTALSLPGDPAAWPAPPPIGRPIDNTRVYVL-----------------DAALRPVPPG---VPGELYIGGAGLAR 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 446 EYWNRPRETEDAFTPDGW------FKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGP 518
Cdd:cd17651 350 GYLNRPELTAERFVPDPFvpgarmYRTGDLARWlPDGELEFLGRAD-DQVKIRGFRIELGEIEAALARHPGVREAVVLAR 428
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2452540125 519 PDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17651 429 EDRPGEKRLVAYVVGDPEAPVDAAELRAALATHLPEYMVPSAFVLLDALPLTPNGKLDRRAL 490
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
39-580 |
2.11e-37 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 149.34 E-value: 2.11e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 39 RGLQTTGAAYSSDVA---PVFTKALAFGDKIAIIDQNGEHTYRDLLSQSLRLSQEIcRALDCSSrdlkEERISFLCPNDA 115
Cdd:PRK12316 1990 ADWDRTPEAYPRGPGvhqRIAEQAARAPEAIAVVFGDQHLSYAELDSRANRLAHRL-RARGVGP----EVRVAIAAERSF 2064
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 116 SYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALVIAAEEYVGKITPSAeklGVPVLPLLRSC--SDGSASHPAV 193
Cdd:PRK12316 2065 ELVVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLLTQRHLLERLPLPA---GVARLPLDRDAewADYPDTAPAV 2141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 194 EEGPLTTcsswknrgAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLhHVHGVINKLLCPLWVG 273
Cdd:PRK12316 2142 QLAGENL--------AYVIYTSGSTGLPKGVAVSHGALVAHCQAAGERYELSPADCELQFMSF-SFDGAHEQWFHPLLNG 2212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 274 ATCVM------LPE-FNAQMVwkkllsSQAPRISVFMAVptiyaklveyydkhFFQPQVQDFVRAFCQENIRLMVSGSAA 346
Cdd:PRK12316 2213 ARVLIrddelwDPEqLYDEME------RHGVTILDFPPV--------------YLQQLAEHAERDGRPPAVRVYCFGGEA 2272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 347 LPVPVLEK-WKSITGHTLLERYGMTEigmALSNPLHgvripGSVGTPLPGVEVCIATETLKDGARSYTIhaqgDESNTQV 425
Cdd:PRK12316 2273 VPAASLRLaWEALRPVYLFNGYGPTE---AVVTPLL-----WKCRPQDPCGAAYVPIGRALGNRRAYIL----DADLNLL 2340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 426 TPGLegqEGELLVRGPSVFREYWNRPRETEDAFTPDGW-------FKTGDTAAYK-DGVYWIKGRtsVD-IIKNGGFKIS 496
Cdd:PRK12316 2341 APGM---AGELYLGGEGLARGYLNRPGLTAERFVPDPFsasgerlYRTGDLARYRaDGVVEYLGR--IDhQVKIRGFRIE 2415
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 497 ALEVERHLLAHPHITDVAVIGpPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVN 576
Cdd:PRK12316 2416 LGEIEARLQAHPAVREAVVVA-QDGASGKQLVAYVVPDDAAEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLD 2494
|
....
gi 2452540125 577 KKEL 580
Cdd:PRK12316 2495 RKAL 2498
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
210-569 |
2.36e-37 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 141.29 E-value: 2.36e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 210 MIIYTSGTTGRPKGVLSTHENV--QAVTTGLVEKWEwkKEDVILHVLPLHHVhGVINKLLCPLWVGATCVMLPEFNAQMV 287
Cdd:cd17636 4 LAIYTAAFSGRPNGALLSHQALlaQALVLAVLQAID--EGTVFLNSGPLFHI-GTLMFTLATFHAGGTNVFVRRVDAEEV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 288 WKKLlssQAPRIS-VFMAVPTIyAKLVEY-----YDKHFFQPQVQdfvrafcqenirlMVSGSAALPVPVLEKWKSITGh 361
Cdd:cd17636 81 LELI---EAERCThAFLLPPTI-DQIVELnadglYDLSSLRSSPA-------------APEWNDMATVDTSPWGRKPGG- 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 362 tllerYGMTEIGMALSNPLHGVRIPGSVGTPLPGVEVCIatetlkdgarsytihaqGDESNTQVTPGlegQEGELLVRGP 441
Cdd:cd17636 143 -----YGQTEVMGLATFAALGGGAIGGAGRPSPLVQVRI-----------------LDEDGREVPDG---EVGEIVARGP 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 442 SVFREYWNRPRETEDAFTpDGWFKTGDTAAYK-DG-VYWI--KGRtsvdIIKNGGFKISALEVERHLLAHPHITDVAVIG 517
Cdd:cd17636 198 TVMAGYWNRPEVNARRTR-GGWHHTNDLGRREpDGsLSFVgpKTR----MIKSGAENIYPAEVERCLRQHPAVADAAVIG 272
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|..
gi 2452540125 518 PPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPR 569
Cdd:cd17636 273 VPDPRWAQSVKAIVVLKPGASVTEAELIEHCRARIASYKKPKSVEFADALPR 324
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
64-580 |
7.37e-37 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 142.39 E-value: 7.37e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcSSRDLKEERISFLC-PNDASYVVAQWASWMSGGIAVPLYKKHPVTE 142
Cdd:cd17652 2 DAPAVVFGDETLTYAELNARANRLA----RLL--AARGVGPERLVALAlPRSAELVVAILAVLKAGAAYLPLDPAYPAER 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 143 LEYVIQDSQSALVIAaeeyvgkitpsaeklgvpvlpllrscsdgSASHPAveegplttcsswknrgaMIIYTSGTTGRPK 222
Cdd:cd17652 76 IAYMLADARPALLLT-----------------------------TPDNLA-----------------YVIYTSGSTGRPK 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 223 GVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHhVHGVINKLLCPLWVGATCVMLPEfnaqmvwkkllssqapriSVF 302
Cdd:cd17652 110 GVVVTHRGLANLAAAQIAAFDVGPGSRVLQFASPS-FDASVWELLMALLAGATLVLAPA------------------EEL 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 303 MAVPTIYAKLVEYYDKHFFQ-PQVQDFVRAFCQENIRLMVSGSAALPVPVLEKWKsiTGHTLLERYGMTE--IGMALSNP 379
Cdd:cd17652 171 LPGEPLADLLREHRITHVTLpPAALAALPPDDLPDLRTLVVAGEACPAELVDRWA--PGRRMINAYGPTEttVCATMAGP 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 380 LHGVRIPgSVGTPLPGVEVCIatetLKDGARsytihaqgdesntQVTPGLEGqegELLVRGPSVFREYWNRPRETEDAFT 459
Cdd:cd17652 249 LPGGGVP-PIGRPVPGTRVYV----LDARLR-------------PVPPGVPG---ELYIAGAGLARGYLNRPGLTAERFV 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 460 PDGW-------FKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVV 531
Cdd:cd17652 308 ADPFgapgsrmYRTGDLARWRaDGQLEFLGRAD-DQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDDRPGDKRLVAYV 386
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 2452540125 532 QLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17652 387 VPAPGAAPTAAELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
64-583 |
7.79e-37 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 144.13 E-value: 7.79e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEIcRALDCSSRDlkeeRISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:PRK13382 58 DRPGLIDELGTLTWRELDERSDALAAAL-QALPIGEPR----VVGIMCRNHRGFVEALLAANRIGADILLLNTSFAGPAL 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAAEEYvgkiTPSAEKL--GVP--VLPLLRSCSDGSASHPAVEEGPL-TTCSSWKNRGAMIIYTSGTT 218
Cdd:PRK13382 133 AEVVTREGVDTVIYDEEF----SATVDRAlaDCPqaTRIVAWTDEDHDLTVEVLIAAHAgQRPEPTGRKGRVILLTSGTT 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 219 GRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGvINKLLCPLWVGATCVMLPEFNAQMVWKKLLSSQAPR 298
Cdd:PRK13382 209 GTPKGARRSGPGGIGTLKAILDRTPWRAEEPTVIVAPMFHAWG-FSQLVLAASLACTIVTRRRFDPEATLDLIDRHRATG 287
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 299 ISVfmaVPTIYAKLVEYydkhffqpqVQDFVRAFCQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGM-ALS 377
Cdd:PRK13382 288 LAV---VPVMFDRIMDL---------PAEVRNRYSGRSLRFAAASGSRMRPDVVIAFMDQFGDVIYNNYNATEAGMiATA 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 378 NPLHGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFREYwnRPRETEDa 457
Cdd:PRK13382 356 TPADLRAAPDTAGRPAEGTEIRIL-----------------DQDFREVPTG---EVGTIFVRNDTQFDGY--TSGSTKD- 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 458 fTPDGWFKTGDTAAYKD-GVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKG 536
Cdd:PRK13382 413 -FHDGFMASGDVGYLDEnGRLFVVGR-DDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKPG 490
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 2452540125 537 EMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQR 583
Cdd:PRK13382 491 ASATPETLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQAR 537
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
64-580 |
1.99e-36 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 141.29 E-value: 1.99e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPV 140
Cdd:cd17643 2 EAVAVVDEDRRLTYGELDARANRLA----RTL----RAEgvgPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPV 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 141 TELEYVIQDSQSALVIaaeeyvgkitpsaeklgvpvlpllrscsdGSASHPAveegplttcsswknrgaMIIYTSGTTGR 220
Cdd:cd17643 74 ERIAFILADSGPSLLL-----------------------------TDPDDLA-----------------YVIYTSGSTGR 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 221 PKGVLSTHENVQAVTTGLVEKWEWKKEDVilhVLPLHH---------VHGvinkllcPLWVGATCVMLPEFNAQ--MVWK 289
Cdd:cd17643 108 PKGVVVSHANVLALFAATQRWFGFNEDDV---WTLFHSyafdfsvweIWG-------ALLHGGRLVVVPYEVARspEDFA 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 290 KLLSSQapRISVFMAVPTIYAKLVEyydkhffqpQVQDFVRAfcQENIRLMVSGSAALPVPVLEKWKSITGH---TLLER 366
Cdd:cd17643 178 RLLRDE--GVTVLNQTPSAFYQLVE---------AADRDGRD--PLALRYVIFGGEALEAAMLRPWAGRFGLdrpQLVNM 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 367 YGMTEIGMALS-NPLHGVRIPGS----VGTPLPGVEVCIATETLKDGARsytihaqgdesntqvtpgleGQEGELLVRGP 441
Cdd:cd17643 245 YGITETTVHVTfRPLDAADLPAAaaspIGRPLPGLRVYVLDADGRPVPP--------------------GVVGELYVSGA 304
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 442 SVFREYWNRPRET-----EDAFTPDG--WFKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDV 513
Cdd:cd17643 305 GVARGYLGRPELTaerfvANPFGGPGsrMYRTGDLARRLpDGELEYLGRAD-EQVKIRGFRIELGEIEAALATHPSVRDA 383
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 514 AVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17643 384 AVIVREDEPGDTRLVAYVVADDGAAADIAELRALLKELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
64-580 |
2.42e-36 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 141.30 E-value: 2.42e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEIcRALDCssrdLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:cd12115 14 DAIALVCGDESLTYAELNRRANRLAARL-RAAGV----GPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPAYPPERL 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIaaeeyvgkITPSAEklgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTGRPKG 223
Cdd:cd12115 89 RFILEDAQARLVL--------TDPDDL--------------------------------------AYVIYTSGSTGRPKG 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 224 VLSTHENVQAVTtglvekwEWKKedvilHVLPLHHVHGV-----------INKLLCPLWVGATCVMlpefnAQMVWKKLL 292
Cdd:cd12115 123 VAIEHRNAAAFL-------QWAA-----AAFSAEELAGVlastsicfdlsVFELFGPLATGGKVVL-----ADNVLALPD 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 293 SSQAPRISVFMAVPTIYAKLVEyydkhffQPQVQDFVRAFCqenirlmVSGSAALPVPVLEKWKSITGHTLLERYGMTE- 371
Cdd:cd12115 186 LPAAAEVTLINTVPSAAAELLR-------HDALPASVRVVN-------LAGEPLPRDLVQRLYARLQVERVVNLYGPSEd 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 372 --IGMALSNPLHGVRIPgSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGLEGqegELLVRGPSVFREYWN 449
Cdd:cd12115 252 ttYSTVAPVPPGASGEV-SIGRPLANTQAYVL-----------------DRALQPVPLGVPG---ELYIGGAGVARGYLG 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 450 RPRETEDAFTPDGW------FKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMV 522
Cdd:cd12115 311 RPGLTAERFLPDPFgpgarlYRTGDLVRWRpDGLLEFLGRAD-NQVKVRGFRIELGEIEAALRSIPGVREAVVVAIGDAA 389
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 2452540125 523 WGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd12115 390 GERRLVAYIVAEPGAAGLVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
64-580 |
8.74e-36 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 140.10 E-value: 8.74e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSqeicRAL-DCSSRdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTE 142
Cdd:cd12114 2 DATAVICGDGTLTYGELAERARRVA----GALkAAGVR--PGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAAR 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 143 LEYVIQDSQSALVIAAEEYvgkitPSAEKLGVPVLPLLRSCSDGSASHPAVEEGPLTTcsswknrgAMIIYTSGTTGRPK 222
Cdd:cd12114 76 REAILADAGARLVLTDGPD-----AQLDVAVFDVLILDLDALAAPAPPPPVDVAPDDL--------AYVIFTSGSTGTPK 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 223 GVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINkLLCPLWVGATCVMLPEFNAQMV--WKKLLssQAPRIS 300
Cdd:cd12114 143 GVMISHRAALNTILDINRRFAVGPDDRVLALSSLSFDLSVYD-IFGALSAGATLVLPDEARRRDPahWAELI--ERHGVT 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 301 VFMAVPTIYAKLVEYYdkhffqPQVQDFVRafcqeNIRL-MVSG---SAALPVPVLEKWKSITGHTLlerYGMTEiGMAL 376
Cdd:cd12114 220 LWNSVPALLEMLLDVL------EAAQALLP-----SLRLvLLSGdwiPLDLPARLRALAPDARLISL---GGATE-ASIW 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 377 SN-------PLHGVRIPgsVGTPLPGvevciatetlkdgARSYTIHAQGDEsntqvTPglEGQEGELLVRGPSVFREYWN 449
Cdd:cd12114 285 SIyhpidevPPDWRSIP--YGRPLAN-------------QRYRVLDPRGRD-----CP--DWVPGELWIGGRGVALGYLG 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 450 RPRETEDAFTPDG----WFKTGDTAAYK-DGVYWIKGRtsVDI-IKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVW 523
Cdd:cd12114 343 DPELTAARFVTHPdgerLYRTGDLGRYRpDGTLEFLGR--RDGqVKVRGYRIELGEIEAALQAHPGVARAVVVVLGDPGG 420
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 524 GQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd12114 421 KRLAAFVVPDNDGTPIAPDALRAFLAQTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
209-584 |
1.66e-35 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 136.71 E-value: 1.66e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKW----EWkkedviLHVLPLHHVHGvINKLLCPLWVGATCVMLPefna 284
Cdd:PRK07824 38 ALVVATSGTTGTPKGAMLTAAALTASADATHDRLggpgQW------LLALPAHHIAG-LQVLVRSVIAGSEPVELD---- 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 285 qmVWKKLLSSQAPRISVFMAVPTIYAKLVEY-YDKHFFQPQVQDFVRAFcqeniRLMVSGSAALPVPVLEKWKSItGHTL 363
Cdd:PRK07824 107 --VSAGFDPTALPRAVAELGGGRRYTSLVPMqLAKALDDPAATAALAEL-----DAVLVGGGPAPAPVLDAAAAA-GINV 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 364 LERYGMTEIGmalsnplhgvriPGSV--GTPLPGVEVCIAtetlkdgarsytihaqgdesntqvtpglegqEGELLVRGP 441
Cdd:PRK07824 179 VRTYGMSETS------------GGCVydGVPLDGVRVRVE-------------------------------DGRIALGGP 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 442 SVFREYWNRPreTEDAFTPDGWFKTGDTAAYKDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDM 521
Cdd:PRK07824 216 TLAKGYRNPV--DPDPFAEPGWFRTDDLGALDDGVLTVLGRAD-DAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDD 292
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2452540125 522 VWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQRF 584
Cdd:PRK07824 293 RLGQRVVAAVVGDGGPAPTLEALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALVRRF 355
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
113-585 |
1.71e-35 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 139.83 E-value: 1.71e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 113 NDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALVIA----AEEYVGKITPSAEKLGVPVLPLLRSCSDGSA 188
Cdd:PRK12406 45 NDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLIAhadlLHGLASALPAGVTVLSVPTPPEIAAAYRISP 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 189 SHPAVEEGpLTTCSSW------------KNRGAMIiYTSGTTGRPKGV---LSTHENVQAVTTGLVEKWEWKKEDVILHV 253
Cdd:PRK12406 125 ALLTPPAG-AIDWEGWlaqqepydgppvPQPQSMI-YTSGTTGHPKGVrraAPTPEQAAAAEQMRALIYGLKPGIRALLT 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 254 LPLHH----VHGvinklLCPLWVGATCVMLPEFNAQmvwkKLLSS-QAPRISVFMAVPTIYAKLVEYYDKhffqpqvqdf 328
Cdd:PRK12406 203 GPLYHsapnAYG-----LRAGRLGGVLVLQPRFDPE----ELLQLiERHRITHMHMVPTMFIRLLKLPEE---------- 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 329 VRA-FCQENIRLMVSGSAALPVPV----LEKWksitGHTLLERYGMTEIG-MALSNPLHGVRIPGSVGTPLPGVEVCIAt 402
Cdd:PRK12406 264 VRAkYDVSSLRHVIHAAAPCPADVkramIEWW----GPVIYEYYGSTESGaVTFATSEDALSHPGTVGKAAPGAELRFV- 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 403 etlkdgarsytihaqGDESNtqvtPGLEGQEGELLVRGPSV--FrEYWNRPrETEDAFTPDGWFKTGDTAAY-KDGVYWI 479
Cdd:PRK12406 339 ---------------DEDGR----PLPQGEIGEIYSRIAGNpdF-TYHNKP-EKRAEIDRGGFITSGDVGYLdADGYLFL 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 480 KGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPT 559
Cdd:PRK12406 398 CDRKR-DMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEPQPGATLDEADIRAQLKARLAGYKVPK 476
|
490 500
....*....|....*....|....*.
gi 2452540125 560 ELIVVEEIPRNQMGKVNKKELLQRFY 585
Cdd:PRK12406 477 HIEIMAELPREDSGKIFKRRLRDPYW 502
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
204-584 |
1.84e-35 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 140.27 E-value: 1.84e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 204 WK----NRGAMIIYTSGTTGRPKGVLSTHE-NV-QAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLwVGATCV 277
Cdd:PRK06018 171 WKtfdeNTAAGMCYTSGTTGDPKGVLYSHRsNVlHALMANNGDALGTSAADTMLPVVPLFHANSWGIAFSAPS-MGTKLV 249
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 278 ML-PEFNAQMVWKkLLSSQapRISVFMAVPTIYAKLVEYYDKHFFQ-PqvqdfvrafcqeNIRLMVSGSAALPVPVLEKW 355
Cdd:PRK06018 250 MPgAKLDGASVYE-LLDTE--KVTFTAGVPTVWLMLLQYMEKEGLKlP------------HLKMVVCGGSAMPRSMIKAF 314
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 356 KSItGHTLLERYGMTE---IGMALSNPLHGVRIPGSV--------GTPLPGVEVCIATETLKDGARsytihaqgdesntq 424
Cdd:PRK06018 315 EDM-GVEVRHAWGMTEmspLGTLAALKPPFSKLPGDArldvlqkqGYPPFGVEMKITDDAGKELPW-------------- 379
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 425 vtpglEGQE-GELLVRGPSVFREYWnrpRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRtSVDIIKNGGFKISALEVER 502
Cdd:PRK06018 380 -----DGKTfGRLKVRGPAVAAAYY---RVDGEILDDDGFFDTGDVATIdAYGYMRITDR-SKDVIKSGGEWISSIDLEN 450
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 503 HLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQ 582
Cdd:PRK06018 451 LAVGHPKVAEAAVIGVYHPKWDERPLLIVQLKPGETATREEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALRE 530
|
..
gi 2452540125 583 RF 584
Cdd:PRK06018 531 QF 532
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
64-580 |
2.59e-35 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 139.00 E-value: 2.59e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcSSRDLKEERI-SFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTE 142
Cdd:cd17655 12 DHTAVVFEDQTLTYRELNERANQLA----RTL--REKGVGPDTIvGIMAERSLEMIVGILGILKAGGAYLPIDPDYPEER 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 143 LEYVIQDSQSALVIAAEEYVGKITpsaeKLGVPVLPLLRSCSDGSAS--HPAVEEGPLttcsswknrgAMIIYTSGTTGR 220
Cdd:cd17655 86 IQYILEDSGADILLTQSHLQPPIA----FIGLIDLLDEDTIYHEESEnlEPVSKSDDL----------AYVIYTSGSTGK 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 221 PKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLH---HVHGVINKLLCplwvGATCVMLPEfnaqmvwKKLLSSQAp 297
Cdd:cd17655 152 PKGVMIEHRGVVNLVEWANKVIYQGEHLRVALFASISfdaSVTEIFASLLS----GNTLYIVRK-------ETVLDGQA- 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 298 risvfmavptiyakLVEYYDKH-----FFQP---QVQDFVRAFCQENIRLMVSGSAALPVPVLEKWKSITGH--TLLERY 367
Cdd:cd17655 220 --------------LTQYIRQNritiiDLTPahlKLLDAADDSEGLSLKHLIVGGEALSTELAKKIIELFGTnpTITNAY 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 368 GMTE------IGMALSNPLHGVRIPgsVGTPLPGVEVCIAtetlkdgarsytihaqgdESNTQVTPglEGQEGELLVRGP 441
Cdd:cd17655 286 GPTEttvdasIYQYEPETDQQVSVP--IGKPLGNTRIYIL------------------DQYGRPQP--VGVAGELYIGGE 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 442 SVFREYWNRPRETEDAFTPDGW------FKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVA 514
Cdd:cd17655 344 GVARGYLNRPELTAEKFVDDPFvpgermYRTGDLARWlPDGNIEFLGRID-HQVKIRGYRIELGEIEARLLQHPDIKEAV 422
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 515 VIGPPDMVWGQRVSA-VVQLRKgemLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17655 423 VIARKDEQGQNYLCAyIVSEKE---LPVAQLREFLARELPDYMIPSYFIKLDEIPLTPNGKVDRKAL 486
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
68-580 |
1.98e-34 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 136.68 E-value: 1.98e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 68 IIDQNGEH-TYRDLLSQSLRLSqeicRAL-DCSSRdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEY 145
Cdd:PRK13390 17 IVAETGEQvSYRQLDDDSAALA----RVLyDAGLR--TGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAPEADY 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 146 VIQDSQSALVIAAEEYVGkitpSAEKLGVPvLPLLRSCS---DGSASHPAVEEG---PLTT--CsswknrGAMIIYTSGT 217
Cdd:PRK13390 91 IVGDSGARVLVASAALDG----LAAKVGAD-LPLRLSFGgeiDGFGSFEAALAGagpRLTEqpC------GAVMLYSSGT 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 218 TGRPKG---------VLSTHENVQAVTTGLvekWEWKKEDVILHVLPLHHVhgvinkllCPL-W------VGATCVMLPE 281
Cdd:PRK13390 160 TGFPKGiqpdlpgrdVDAPGDPIVAIARAF---YDISESDIYYSSAPIYHA--------APLrWcsmvhaLGGTVVLAKR 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 282 FNAQMVWKKLlssQAPRISVFMAVPTIYAKLVeyydkhffqpQVQDFVRA-FCQENIRLMVSGSAALPVPVLEKWKSITG 360
Cdd:PRK13390 229 FDAQATLGHV---ERYRITVTQMVPTMFVRLL----------KLDADVRTrYDVSSLRAVIHAAAPCPVDVKHAMIDWLG 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 361 HTLLERYGMTEI-GMALSNPLHGVRIPGSVGTPLPGvevciatetlkdgarsyTIHAQGDESNTqvTPGleGQEGELLVR 439
Cdd:PRK13390 296 PIVYEYYSSTEAhGMTFIDSPDWLAHPGSVGRSVLG-----------------DLHICDDDGNE--LPA--GRIGTVYFE 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 440 GPSVFREYWNRPRETEDAFTPDG--WFKTGDTAAY-KDGVYWIKGRTSVDIIkNGGFKISALEVERHLLAHPHITDVAVI 516
Cdd:PRK13390 355 RDRLPFRYLNDPEKTAAAQHPAHpfWTTVGDLGSVdEDGYLYLADRKSFMII-SGGVNIYPQETENALTMHPAVHDVAVI 433
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2452540125 517 GPPDMVWGQRVSAVVQLRKG----EMLSvKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK13390 434 GVPDPEMGEQVKAVIQLVEGirgsDELA-RELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLL 500
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
213-583 |
2.11e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 137.00 E-value: 2.11e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 213 YTSGTTGRPKGVLSTHEN--VQAVTTGLVekWEWKKEDVILHVLPLHHVHGvinklLCPLW----VGATCVMLPEFNAQM 286
Cdd:PRK08162 189 YTSGTTGNPKGVVYHHRGayLNALSNILA--WGMPKHPVYLWTLPMFHCNG-----WCFPWtvaaRAGTNVCLRKVDPKL 261
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 287 VWKKLlssQAPRISVFMAVPTIYAKLVEYYDkhffqpqvqDFVRAFCQEnIRLMVSGsAALPVPVLEKWKSItGHTLLER 366
Cdd:PRK08162 262 IFDLI---REHGVTHYCGAPIVLSALINAPA---------EWRAGIDHP-VHAMVAG-AAPPAAVIAKMEEI-GFDLTHV 326
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 367 YGMTEIgmalsnplHGvriPGSVGTPLPGVEvciateTLKDGARSyTIHA-QG------------DESNTQVTPGlEGQE 433
Cdd:PRK08162 327 YGLTET--------YG---PATVCAWQPEWD------ALPLDERA-QLKArQGvryplqegvtvlDPDTMQPVPA-DGET 387
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 434 -GELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTAA-YKDGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHIT 511
Cdd:PRK08162 388 iGEIMFRGNIVMKGYLKNPKATEEAFA-GGWFHTGDLAVlHPDGYIKIKDR-SKDIIISGGENISSIEVEDVLYRHPAVL 465
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2452540125 512 DVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTElIVVEEIPRNQMGKVNKKELLQR 583
Cdd:PRK08162 466 VAAVVAKPDPKWGEVPCAFVELKDGASATEEEIIAHCREHLAGFKVPKA-VVFGELPKTSTGKIQKFVLREQ 536
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
59-491 |
2.44e-34 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 136.98 E-value: 2.44e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIA---IIDQNGE---HTYRDLLSQSLRLSQEIcraldcSSRDLKEERISFLCPNDASYVVAQWASWMSGGIAV 132
Cdd:cd05931 3 AAARPDRPAytfLDDEGGReetLTYAELDRRARAIAARL------QAVGKPGDRVLLLAPPGLDFVAAFLGCLYAGAIAV 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 133 PLYKKHPVTE---LEYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLPLLRSC----SDGSASHPAVEEGPLTTcsswk 205
Cdd:cd05931 77 PLPPPTPGRHaerLAAILADAGPRVVLTTAAALAAVRAFAASRPAAGTPRLLVVdllpDTSAADWPPPSPDPDDI----- 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 206 nrgAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLP--EFN 283
Cdd:cd05931 152 ---AYLQYTSGSTGTPKGVVVTHRNLLANVRQIRRAYGLDPGDVVVSWLPLYHDMGLIGGLLTPLYSGGPSVLMSpaAFL 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 284 AQ-MVWKKLLS-SQAprisVFMAVPT---------IYAKLVEYYD----KHFF---QP----QVQDFVRAFCQENIR--- 338
Cdd:cd05931 229 RRpLRWLRLISrYRA----TISAAPNfaydlcvrrVRDEDLEGLDlsswRVALngaEPvrpaTLRRFAEAFAPFGFRpea 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 339 -----------LMVSGSAALPVPVLEkwksitghtLLERygmteigMALSNPLHGVRIPG-------SVGTPLPGVEVCI 400
Cdd:cd05931 305 frpsyglaeatLFVSGGPPGTGPVVL---------RVDR-------DALAGRAVAVAADDpaarelvSCGRPLPDQEVRI 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 401 AtetlkdgarsytihaqgDESNTQVTPglEGQEGELLVRGPSVFREYWNRPRETEDAFTP------DGWFKTGDTAAYKD 474
Cdd:cd05931 369 V-----------------DPETGRELP--DGEVGEIWVRGPSVASGYWGRPEATAETFGAlaatdeGGWLRTGDLGFLHD 429
|
490
....*....|....*..
gi 2452540125 475 GVYWIKGRTSVDIIKNG 491
Cdd:cd05931 430 GELYITGRLKDLIIVRG 446
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
211-576 |
6.88e-34 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 132.51 E-value: 6.88e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 211 IIYTSGTTGRPKGVLSTHE-------NVQAVTTGLVEKWEW------KKED-VILHVLPLHHVHGVINKLLCPLWVGATC 276
Cdd:cd05924 8 ILYTGGTTGMPKGVMWRQEdifrmlmGGADFGTGEFTPSEDahkaaaAAAGtVMFPAPPLMHGTGSWTAFGGLLGGQTVV 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 277 VMLPEFNAQMVWKKLLSSQAPRISVF---MAVPtiyakLVEYYDKhffqpqvqdfVRAFCQENIRLMVSGSAALPVPVLE 353
Cdd:cd05924 88 LPDDRFDPEEVWRTIEKHKVTSMTIVgdaMARP-----LIDALRD----------AGPYDLSSLFAISSGGALLSPEVKQ 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 354 KWKSITGH-TLLERYGMTEIGMalsnplhgvripGSVGTPLPGVevciatetlkDGARSYTIHAQG----DESNTQVTPG 428
Cdd:cd05924 153 GLLELVPNiTLVDAFGSSETGF------------TGSGHSAGSG----------PETGPFTRANPDtvvlDDDGRVVPPG 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 429 lEGQEGELLVRGpSVFREYWNRPRETEDAF-TPDG--WFKTGDTAAY-KDGVYWIKGRTSVdIIKNGGFKISALEVERHL 504
Cdd:cd05924 211 -SGGVGWIARRG-HIPLGYYGDEAKTAETFpEVDGvrYAVPGDRATVeADGTVTLLGRGSV-CINTGGEKVFPEEVEEAL 287
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2452540125 505 LAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVN 576
Cdd:cd05924 288 KSHPAVYDVLVVGRPDERWGQEVVAVVQLREGAGVDLEELREHCRTRIARYKLPKQVVFVDEIERSPAGKAD 359
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
201-520 |
1.32e-33 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 134.26 E-value: 1.32e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 201 CSSWKNRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEK-WEWKKE-DVILHVLPLHHVHGVINKLLCPLWVGATCVM 278
Cdd:cd17639 83 TDGKPDDLACIMYTSGSTGNPKGVMLTHGNLVAGIAGLGDRvPELLGPdDRYLAYLPLAHIFELAAENVCLYRGGTIGYG 162
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 279 LPefnaqmvwKKLLSS---------QAPRISVFMAVPTIY------------------------------AKLVEYYDKH 319
Cdd:cd17639 163 SP--------RTLTDKskrgckgdlTEFKPTLMVGVPAIWdtirkgvlaklnpmgglkrtlfwtayqsklKALKEGPGTP 234
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 320 FFQPQVQDFVRAFCQENIRLMVSGSAALPvPVLEKWKSITGHTLLERYGMTEI--GMALSNPLHgvRIPGSVGTPLPGVE 397
Cdd:cd17639 235 LLDELVFKKVRAALGGRLRYMLSGGAPLS-ADTQEFLNIVLCPVIQGYGLTETcaGGTVQDPGD--LETGRVGPPLPCCE 311
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 398 VciateTLKD--GARSYTihaqgDESNTQvtpglegqeGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KD 474
Cdd:cd17639 312 I-----KLVDweEGGYST-----DKPPPR---------GEILIRGPNVFKGYYKNPEKTKEAFDGDGWFHTGDIGEFhPD 372
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 2452540125 475 GVYWIKGRTSvDIIKNGGFKISALE-VERHLLAHPHITDVAVIGPPD 520
Cdd:cd17639 373 GTLKIIDRKK-DLVKLQNGEYIALEkLESIYRSNPLVNNICVYADPD 418
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
76-585 |
3.01e-33 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 133.28 E-value: 3.01e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSQEIcraldcSSRDLKE-ERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSAL 154
Cdd:PRK13391 26 TYRELDERSNRLAHLF------RSLGLKRgDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARA 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 155 VIAAEEYvGKITPSAEKLgvpvLPLLRSC--------SDGSASHPAVEEGPLTTCSSWKNRGAMIIYTSGTTGRPKGVLS 226
Cdd:PRK13391 100 LITSAAK-LDVARALLKQ----CPGVRHRlvldgdgeLEGFVGYAEAVAGLPATPIADESLGTDMLYSSGTTGRPKGIKR 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 227 --THENVQAVT--TGLVEK-WEWKKEDVILHVLPLHHVH-----GVINKLlcplwvGATCVMLPEFNAQmvwKKLLSSQA 296
Cdd:PRK13391 175 plPEQPPDTPLplTAFLQRlWGFRSDMVYLSPAPLYHSApqravMLVIRL------GGTVIVMEHFDAE---QYLALIEE 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 297 PRISVFMAVPTIYAKLVEYydkhffqPqvqdfvrafcqENIR-------LMVSGSAALPVPVLEKWKSIT--GHTLLERY 367
Cdd:PRK13391 246 YGVTHTQLVPTMFSRMLKL-------P-----------EEVRdkydlssLEVAIHAAAPCPPQVKEQMIDwwGPIIHEYY 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 368 GMTE-IGMALSNPLHGVRIPGSVGTPLPG-VEVCiatetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRGPSVFr 445
Cdd:PRK13391 308 AATEgLGFTACDSEEWLAHPGTVGRAMFGdLHIL-------------------DDDGAELPPG---EPGTIWFEGGRPF- 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 446 EYWNRPRETEDAFTPDG-WFKTGDTAaY--KDGVYWIKGRTSVDIIkNGGFKISALEVERHLLAHPHITDVAVIGPPDMV 522
Cdd:PRK13391 365 EYLNDPAKTAEARHPDGtWSTVGDIG-YvdEDGYLYLTDRAAFMII-SGGVNIYPQEAENLLITHPKVADAAVFGVPNED 442
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 523 WGQRVSAVVQLRKGEMLSV---KDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQRFY 585
Cdd:PRK13391 443 LGEEVKAVVQPVDGVDPGPalaAELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLRDRYW 508
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
43-582 |
4.21e-33 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 133.33 E-value: 4.21e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 43 TTGAAYSSDVAPVFTKALAFG--DKIAIIDQNGEHTYRDLLSQSLRLSQEICRALDCssrdlKEERISFLCPNDASYVVA 120
Cdd:PRK06164 2 PHDAAPRADTLASLLDAHARArpDAVALIDEDRPLSRAELRALVDRLAAWLAAQGVR-----RGDRVAVWLPNCIEWVVL 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 121 QWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALVIAAEEYVG-------KITPSAEKLGVPVLPLLRSCSDGSASH--- 190
Cdd:PRK06164 77 FLACARLGATVIAVNTRYRSHEVAHILGRGRARWLVVWPGFKGidfaailAAVPPDALPPLRAIAVVDDAADATPAPapg 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 191 -----PAVEEGPLTTCSSWK----NRGAMIIYTSGTTGRPKGV------LSTHENVQAVTTGLvekwewKKEDVILHVLP 255
Cdd:PRK06164 157 arvqlFALPDPAPPAAAGERaadpDAGALLFTTSGTTSGPKLVlhrqatLLRHARAIARAYGY------DPGAVLLAALP 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 256 LHHVHGvINKLLCPLWVGATCVMLPEFNAQMVWKKLLSSqapRISVFMAVPTIYAKLveyydkHFFQPQVQDFVRAfcqe 335
Cdd:PRK06164 231 FCGVFG-FSTLLGALAGGAPLVCEPVFDAARTARALRRH---RVTHTFGNDEMLRRI------LDTAGERADFPSA---- 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 336 niRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTE-IGMALSNPLH---GVRIPGSvGTPL-PGVEVCIAtetlkdgar 410
Cdd:PRK06164 297 --RLFGFASFAPALGELAALARARGVPLTGLYGSSEvQALVALQPATdpvSVRIEGG-GRPAsPEARVRAR--------- 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 411 sytihaqgDESNTQVTPglEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTA-AYKDGVYWIKGRTSvDIIK 489
Cdd:PRK06164 365 --------DPQDGALLP--DGESGEIEIRAPSLMRGYLDNPDATARALTDDGYFRTGDLGyTRGDGQFVYQTRMG-DSLR 433
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 490 NGGFKISALEVERHLLAHPHITDVAVIGPpdMVWGQ-RVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIP 568
Cdd:PRK06164 434 LGGFLVNPAEIEHALEALPGVAAAQVVGA--TRDGKtVPVAFVIPTDGASPDEAGLMAACREALAGFKVPARVQVVEAFP 511
|
570
....*....|....*..
gi 2452540125 569 RNQMG---KVNKKELLQ 582
Cdd:PRK06164 512 VTESAngaKIQKHRLRE 528
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
76-468 |
6.04e-33 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 132.72 E-value: 6.04e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSQEICRALDCSSrdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALV 155
Cdd:cd05927 7 SYKEVAERADNIGSALRSLGGKPA---PASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEISIV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 156 IAAEeyvgkitpsaeklGVPVLPLLRSCSDGSASHPAVEEG-PLTTCSswknrgamIIYTSGTTGRPKGVLSTHENVQAV 234
Cdd:cd05927 84 FCDA-------------GVKVYSLEEFEKLGKKNKVPPPPPkPEDLAT--------ICYTSGTTGNPKGVMLTHGNIVSN 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 235 TTGLVEKWE----WKKEDVILHVLPLHHV--HGVINKLLC-----PLWVGATCVMLPEFnaqmvwkkllssQAPRISVFM 303
Cdd:cd05927 143 VAGVFKILEilnkINPTDVYISYLPLAHIfeRVVEALFLYhgakiGFYSGDIRLLLDDI------------KALKPTVFP 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 304 AVPTIYAKLveyYDKHFFQPQVQDFVRAFC-------------------------------QE----NIRLMVSGSAALP 348
Cdd:cd05927 211 GVPRVLNRI---YDKIFNKVQAKGPLKRKLfnfalnyklaelrsgvvraspfwdklvfnkiKQalggNVRLMLTGSAPLS 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 349 VPVLEKWKSITGHTLLERYGMTEI--GMALSNPlhGVRIPGSVGTPLPGVEVciateTLKDGArsytihaqgdESNTQVT 426
Cdd:cd05927 288 PEVLEFLRVALGCPVLEGYGQTECtaGATLTLP--GDTSVGHVGGPLPCAEV-----KLVDVP----------EMNYDAK 350
|
410 420 430 440
....*....|....*....|....*....|....*....|..
gi 2452540125 427 PglEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGD 468
Cdd:cd05927 351 D--PNPRGEVCIRGPNVFSGYYKDPEKTAEALDEDGWLHTGD 390
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
58-585 |
2.08e-32 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 133.93 E-value: 2.08e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 58 KALAFGDKIAIIDQNGEHTYRDLLSQSLRLSQE-ICRALDcssrdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYK 136
Cdd:PRK12316 4560 RARMTPDAVAVVFDEEKLTYAELNRRANRLAHAlIARGVG------PEVLVGIAMERSAEMMVGLLAVLKAGGAYVPLDP 4633
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 137 KHPVTELEYVIQDSQSALVIAAEEYVGKItPSAEKLGVPVLPLLRSCSDGSASHPAVEEGPlttcsswkNRGAMIIYTSG 216
Cdd:PRK12316 4634 EYPRERLAYMMEDSGAALLLTQSHLLQRL-PIPDGLASLALDRDEDWEGFPAHDPAVRLHP--------DNLAYVIYTSG 4704
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 217 TTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLhHVHGVINKLLCPLWVGATCVM------LPEFNAQMVWKK 290
Cdd:PRK12316 4705 STGRPKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSF-SFDGSHEGLYHPLINGASVVIrddslwDPERLYAEIHEH 4783
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 291 llssqapRISVFMAVPTiyaklveyydkhFFQPQVQDFVRAFCQENIRLMVSGSAALPVPVLEK-WKSITGHTLLERYGM 369
Cdd:PRK12316 4784 -------RVTVLVFPPV------------YLQQLAEHAERDGEPPSLRVYCFGGEAVAQASYDLaWRALKPVYLFNGYGP 4844
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 370 TEigmALSNPLH----GVRIPGS----VGTPLPGVevciATETLKDgarsytihaqgdesntQVTPGLEGQEGELLVRGP 441
Cdd:PRK12316 4845 TE---TTVTVLLwkarDGDACGAaympIGTPLGNR----SGYVLDG----------------QLNPLPVGVAGELYLGGE 4901
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 442 SVFREYWNRPRETEDAFTPDGW-------FKTGDTAAYK-DGVYWIKGRtsVD-IIKNGGFKISALEVERHLLAHPHITD 512
Cdd:PRK12316 4902 GVARGYLERPALTAERFVPDPFgapggrlYRTGDLARYRaDGVIDYLGR--VDhQVKIRGFRIELGEIEARLREHPAVRE 4979
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 513 VAVIGPPDMVWGQRV-------SAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL----- 580
Cdd:PRK12316 4980 AVVIAQEGAVGKQLVgyvvpqdPALADADEAQAELRDELKAALRERLPEYMVPAHLVFLARMPLTPNGKLDRKALpqpda 5059
|
....*..
gi 2452540125 581 --LQRFY 585
Cdd:PRK12316 5060 slLQQAY 5066
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
63-575 |
6.72e-32 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 130.45 E-value: 6.72e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 63 GDKIAII---DQNGEH---TYRDLLsqslrlsQEICR---ALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGI 130
Cdd:TIGR02188 71 PDKVAIIwegDEPGEVrkiTYRELH-------REVCRfanVL----KSLgvkKGDRVAIYMPMIPEAAIAMLACARIGAI 139
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 131 avplykkHPVT-------ELEYVIQDSQSALVIAAEEYV--GKITP--------------SAEK------LGVPVLP--- 178
Cdd:TIGR02188 140 -------HSVVfggfsaeALADRINDAGAKLVITADEGLrgGKVIPlkaivdealekcpvSVEHvlvvrrTGNPVVPwve 212
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 179 --------LLRSCSDGSASHPAVEEGPLttcsswknrgaMIIYTSGTTGRPKGVLSTHE--NVQAVTTGlveKW--EWKK 246
Cdd:TIGR02188 213 grdvwwhdLMAKASAYCEPEPMDSEDPL-----------FILYTSGSTGKPKGVLHTTGgyLLYAAMTM---KYvfDIKD 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 247 EDVILHVLPLHHVHGVINKLLCPLWVGATCVML---PEF-NAQMVWKKLlssQAPRISVFMAVPTIYAKLVEYYDkhffq 322
Cdd:TIGR02188 279 GDIFWCTADVGWITGHSYIVYGPLANGATTVMFegvPTYpDPGRFWEII---EKHKVTIFYTAPTAIRALMRLGD----- 350
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 323 pqvqDFVRAFCQENIRLMvsGSAALPV-PvlEKWK---SITGHT---LLERYGMTEIGMALSNPLHGV--RIPGSVGTPL 393
Cdd:TIGR02188 351 ----EWVKKHDLSSLRLL--GSVGEPInP--EAWMwyyKVVGKErcpIVDTWWQTETGGIMITPLPGAtpTKPGSATLPF 422
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 394 PGVEVCIATETlkdgarsytihaqgdesnTQVTPGlEGQEGELLVRG--PSVFREYWNRPRETEDAFTPD--GWFKTGDT 469
Cdd:TIGR02188 423 FGIEPAVVDEE------------------GNPVEG-PGEGGYLVIKQpwPGMLRTIYGDHERFVDTYFSPfpGYYFTGDG 483
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 470 AAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLS---VKDLK 545
Cdd:TIGR02188 484 ARRdKDGYIWITGRVD-DVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTLKDGYEPDdelRKELR 562
|
570 580 590
....*....|....*....|....*....|
gi 2452540125 546 DWARDTMAPYTIPTELIVVEEIPRNQMGKV 575
Cdd:TIGR02188 563 KHVRKEIGPIAKPDKIRFVPGLPKTRSGKI 592
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
55-583 |
7.30e-32 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 129.71 E-value: 7.30e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 55 VFTKALAFGDKIAIID--QNGEHTYRDLLSQSLRLSQEIcRALDCSsrdlKEERISFLCPNDASYVVAQWASWMSGGIAV 132
Cdd:PLN02330 34 VLQDAELYADKVAFVEavTGKAVTYGEVVRDTRRFAKAL-RSLGLR----KGQVVVVVLPNVAEYGIVALGIMAAGGVFS 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 133 PLYKKHPVTELEYVIQDSQSALVIAAEEYVGKItpsaEKLGVPVLPLLRSCSDGS------------ASHPAVEEGPLTT 200
Cdd:PLN02330 109 GANPTALESEIKKQAEAAGAKLIVTNDTNYGKV----KGLGLPVIVLGEEKIEGAvnwkelleaadrAGDTSDNEEILQT 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 201 --CSswknrgamIIYTSGTTGRPKGVLSTHENVQA--VTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATC 276
Cdd:PLN02330 185 dlCA--------LPFSSGTTGISKGVMLTHRNLVAnlCSSLFSVGPEMIGQVVTLGLIPFFHIYGITGICCATLRNKGKV 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 277 VMLPEFNAQMVWKKLLSSQaprISVFMAVPTIYAKLVEyydkhffQPQVQDFvrAFCQENIRLMVSGSAALPVPVLEKW- 355
Cdd:PLN02330 257 VVMSRFELRTFLNALITQE---VSFAPIVPPIILNLVK-------NPIVEEF--DLSKLKLQAIMTAAAPLAPELLTAFe 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 356 KSITGHTLLERYGMTE---IGMALSNPL--HGVRIPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPglE 430
Cdd:PLN02330 325 AKFPGVQVQEAYGLTEhscITLTHGDPEkgHGIAKKNSVGFILPNLEVKFI-----------------DPDTGRSLP--K 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 431 GQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPH 509
Cdd:PLN02330 386 NTPGELCVRSQCVMQGYYNNKEETDRTIDEDGWLHTGDIGYIdDDGDIFIVDRIK-ELIKYKGFQVAPAELEAILLTHPS 464
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2452540125 510 ITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQR 583
Cdd:PLN02330 465 VEDAAVVPLPDEEAGEIPAACVVINPKAKESEEDILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKEK 538
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
104-584 |
1.37e-31 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 129.13 E-value: 1.37e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 104 EERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALVIAAEEyvgkitpSAEKLGvpvlPLLRSC 183
Cdd:PRK05620 64 DQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDEVIVADPR-------LAEQLG----EILKEC 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 184 ---------SDGSASHPAVEEGPLTTCSSWK-----------------NRGAMIIYTSGTTGRPKGVLSTHENV--QAVT 235
Cdd:PRK05620 133 pcvravvfiGPSDADSAAAHMPEGIKVYSYEalldgrstvydwpeldeTTAAAICYSTGTTGAPKGVVYSHRSLylQSLS 212
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 236 TGLVEKWEWKKEDVILHVLPLHHV--HGVinkllcPL--WVGATCVMLPEFNAQM-VWKKLLSSQAPRISvfMAVPTIYA 310
Cdd:PRK05620 213 LRTTDSLAVTHGESFLCCVPIYHVlsWGV------PLaaFMSGTPLVFPGPDLSApTLAKIIATAMPRVA--HGVPTLWI 284
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 311 KLVEYYDKHffQPQvqdfvRAFCQEnirLMVSGSAALPVpVLEKWKsitghtllERYGMTEI---GMALSNPLhgvripG 387
Cdd:PRK05620 285 QLMVHYLKN--PPE-----RMSLQE---IYVGGSAVPPI-LIKAWE--------ERYGVDVVhvwGMTETSPV------G 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 388 SVGTPLPGVevciATETlkdgARSYTIhAQGD---------ESNTQVTPGLEGQEGELLVRGPSVFREYWNRPRETEDA- 457
Cdd:PRK05620 340 TVARPPSGV----SGEA----RWAYRV-SQGRfpasleyriVNDGQVMESTDRNEGEIQVRGNWVTASYYHSPTEEGGGa 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 458 ---------------FTPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDM 521
Cdd:PRK05620 411 astfrgedvedandrFTADGWLRTGDVGSVtRDGFLTIHDRAR-DVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDD 489
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 522 VWGQRVSAVVQLRKG---EMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQRF 584
Cdd:PRK05620 490 KWGERPLAVTVLAPGiepTRETAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSVGKFDKKDLRQHL 555
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
63-575 |
5.07e-31 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 127.69 E-value: 5.07e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 63 GDKIAIIDQNGE------HTYRDLLSQSLRLSQEIcraldcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVP 133
Cdd:cd17634 67 GDRTAIIYEGDDtsqsrtISYRELHREVCRFAGTL--------LDLgvkKGDRVAIYMPMIPEAAVAMLACARIGAVHSV 138
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 134 LYKKHPVTELEYVIQDSQSALVIAAEEYV--GKITP------SAEKLGVP----VLPLLRSCS----------------- 184
Cdd:cd17634 139 IFGGFAPEAVAGRIIDSSSRLLITADGGVraGRSVPlkknvdDALNPNVTsvehVIVLKRTGSdidwqegrdlwwrdlia 218
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 185 DGSASHPAVEEGPLTTCsswknrgaMIIYTSGTTGRPKGVLSTHEN-VQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVI 263
Cdd:cd17634 219 KASPEHQPEAMNAEDPL--------FILYTSGTTGKPKGVLHTTGGyLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHS 290
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 264 NKLLCPLWVGATCVML---PEF-NAQMVWKKLLSSQaprISVFMAVPTIYAKLveyydkhffQPQVQDFVRAFCQENIRL 339
Cdd:cd17634 291 YLLYGPLACGATTLLYegvPNWpTPARMWQVVDKHG---VNILYTAPTAIRAL---------MAAGDDAIEGTDRSSLRI 358
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 340 MVS-GSAALPVPVLEKWKSITGH--TLLERYGMTEIGMALSNPLHGVR--IPGSVGTPLPGVEVCIATEtlkdgarsyti 414
Cdd:cd17634 359 LGSvGEPINPEAYEWYWKKIGKEkcPVVDTWWQTETGGFMITPLPGAIelKAGSATRPVFGVQPAVVDN----------- 427
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 415 haqgdESNTQvtPGleGQEGELLVRG--PSVFREYWNRPRETEDAF--TPDGWFKTGDTAAY-KDGVYWIKGRtSVDIIK 489
Cdd:cd17634 428 -----EGHPQ--PG--GTEGNLVITDpwPGQTRTLFGDHERFEQTYfsTFKGMYFSGDGARRdEDGYYWITGR-SDDVIN 497
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 490 NGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLS---VKDLKDWARDTMAPYTIPTELIVVEE 566
Cdd:cd17634 498 VAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSpelYAELRNWVRKEIGPLATPDVVHWVDS 577
|
....*....
gi 2452540125 567 IPRNQMGKV 575
Cdd:cd17634 578 LPKTRSGKI 586
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
76-584 |
1.09e-30 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 124.94 E-value: 1.09e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSQEIcRALDCSSRDlkeeRISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALV 155
Cdd:cd05973 2 TFGELRALSARFANAL-QELGVGPGD----VVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLV 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 156 IAAEEYVGKITpsaeklgvpvlpllrscsdgsashpaveEGPLttcsswknrgaMIIYTSGTTGRPKGVLSTHENVQAVT 235
Cdd:cd05973 77 VTDAANRHKLD----------------------------SDPF-----------VMMFTSGTTGLPKGVPVPLRALAAFG 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 236 TGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLP-EFNAQMVWKKLlssQAPRISVFMAVPTIYAKLVe 314
Cdd:cd05973 118 AYLRDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPTILLEgGFSVESTWRVI---ERLGVTNLAGSPTAYRLLM- 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 315 yydkhffqpqvQDFVRAFCQENIRLMVSGSAALPV-PVLEKWKSIT-GHTLLERYGMTEIGMALSN---PLHGVRIpGSV 389
Cdd:cd05973 194 -----------AAGAEVPARPKGRLRRVSSAGEPLtPEVIRWFDAAlGVPIHDHYGQTELGMVLANhhaLEHPVHA-GSA 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 390 GTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGLEGQEGELLVRGPSV-FREYWNRPRETEDAftpdGWFKTGD 468
Cdd:cd05973 262 GRAMPGWRVAVL-----------------DDDGDELGPGEPGRLAIDIANSPLMwFRGYQLPDTPAIDG----GYYLTGD 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 469 TAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLS---VKDL 544
Cdd:cd05973 321 TVEFDpDGSFSFIGRAD-DVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTpalADEL 399
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 2452540125 545 KDWARDTMAPYTIPTELIVVEEIPRNQMGKVnkkellQRF 584
Cdd:cd05973 400 QLHVKKRLSAHAYPRTIHFVDELPKTPSGKI------QRF 433
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
58-584 |
1.12e-30 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 125.35 E-value: 1.12e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 58 KALAFGDKIAIIDQNGEHTYRDLLSQSLRLSQEICraldcsSRDLKEERISFLCPnDAS--YVVAQWASWMSGGIAVPLY 135
Cdd:cd05918 8 RARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLR------SLGVGPGVFVPLCF-EKSkwAVVAMLAVLKAGGAFVPLD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 136 KKHPVTELEYVIQDSQSALVIAAeeyvgkitpsaeklgvpvlpllrscsdgSASHPAVeegplttcsswknrgamIIYTS 215
Cdd:cd05918 81 PSHPLQRLQEILQDTGAKVVLTS----------------------------SPSDAAY-----------------VIFTS 115
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 216 GTTGRPKGVLSTHenvQAVTTGlvekwewkkedvILHVLPLHHVHGV--------------INKLLCPLWVGAT-CVM-- 278
Cdd:cd05918 116 GSTGKPKGVVIEH---RALSTS------------ALAHGRALGLTSEsrvlqfasytfdvsILEIFTTLAAGGClCIPse 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 279 ------LPEFNAQMvwkkllssqapRISVFMAVPTIyAKLVeyydkhffQPQvqDFVrafcqeNIRLMVSGSAALPVPVL 352
Cdd:cd05918 181 edrlndLAGFINRL-----------RVTWAFLTPSV-ARLL--------DPE--DVP------SLRTLVLGGEALTQSDV 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 353 EKWKSitGHTLLERYGMTE--IGMALSNPLHGVRiPGSVGTPLPGVevCIATETlkdgarsytihaqgdESNTQVTPglE 430
Cdd:cd05918 233 DTWAD--RVRLINAYGPAEctIAATVSPVVPSTD-PRNIGRPLGAT--CWVVDP---------------DNHDRLVP--I 290
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 431 GQEGELLVRGPSVFREYWNRPRETEDAFTPD-GW------------FKTGDTAAY-KDG-VYWIkGRTSvDIIKNGGFKI 495
Cdd:cd05918 291 GAVGELLIEGPILARGYLNDPEKTAAAFIEDpAWlkqegsgrgrrlYRTGDLVRYnPDGsLEYV-GRKD-TQVKIRGQRV 368
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 496 SALEVERHLLAHPHITD---VAVIGPPDMVWGQRVSAVVQLRKGEMLS-----------------VKDLKDWARDTMAPY 555
Cdd:cd05918 369 ELGEIEHHLRQSLPGAKevvVEVVKPKDGSSSPQLVAFVVLDGSSSGSgdgdslflepsdefralVAELRSKLRQRLPSY 448
|
570 580
....*....|....*....|....*....
gi 2452540125 556 TIPTELIVVEEIPRNQMGKVNKKELLQRF 584
Cdd:cd05918 449 MVPSVFLPLSHLPLTASGKIDRRALRELA 477
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
213-580 |
1.77e-30 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 125.90 E-value: 1.77e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 213 YTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGvinkllcplWV--------GATCVMLPEFNA 284
Cdd:PLN03102 193 YTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCNG---------WTftwgtaarGGTSVCMRHVTA 263
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 285 QMVWKKLlssQAPRISVFMAVPTIYAKLVE--YYDKHFFQPQVQdfvrafcqenirlMVSGSAALPVPVLEKWKSItGHT 362
Cdd:PLN03102 264 PEIYKNI---EMHNVTHMCCVPTVFNILLKgnSLDLSPRSGPVH-------------VLTGGSPPPAALVKKVQRL-GFQ 326
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 363 LLERYGMTEIgmalSNPL-------HGVRIPGSVGTPLPGvevciatetlKDGARSYTIhAQGDESNTQVTPGL--EGQE 433
Cdd:PLN03102 327 VMHAYGLTEA----TGPVlfcewqdEWNRLPENQQMELKA----------RQGVSILGL-ADVDVKNKETQESVprDGKT 391
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 434 -GELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTAA-YKDGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHIT 511
Cdd:PLN03102 392 mGEIVIKGSSIMKGYLKNPKATSEAFK-HGWLNTGDVGViHPDGHVEIKDR-SKDIIISGGENISSVEVENVLYKYPKVL 469
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2452540125 512 DVAVIGPPDMVWGQRVSAVVQLRKGE----------MLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PLN03102 470 ETAVVAMPHPTWGETPCAFVVLEKGEttkedrvdklVTRERDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKL 548
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
61-582 |
4.34e-30 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 122.80 E-value: 4.34e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 61 AFGDKIAIIDQNGEHTYRDLLSQSLRLSQEICRALDCSSrdlkeERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPV 140
Cdd:cd17653 9 AHPDAVAVESLGGSLTYGELDAASNALANRLLQLGVVPG-----DVVPLLSDRSLEMLVAILAILKAGAAYVPLDAKLPS 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 141 TELEYVIQDSQSALVIaaeeyvgkitpsaeklgvpvlpllrscsdgsashpaveegplttCSSWKNRGAMIIYTSGTTGR 220
Cdd:cd17653 84 ARIQAILRTSGATLLL--------------------------------------------TTDSPDDLAYIIFTSGSTGI 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 221 PKGVLSTHENVQAVTtglvekwewKKEDVILHVLP---LHHVHGV-----INKLLCPLWVGATCVML-PEFNaqmvwkkl 291
Cdd:cd17653 120 PKGVMVPHRGVLNYV---------SQPPARLDVGPgsrVAQVLSIafdacIGEIFSTLCNGGTLVLAdPSDP-------- 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 292 LSSQAPRISVFMAVPTIYAKLveyydkhffqpQVQDFvrafcqENIRLMVSGSAALPVPVLEKWKSitGHTLLERYGMTE 371
Cdd:cd17653 183 FAHVARTVDALMSTPSILSTL-----------SPQDF------PNLKTIFLGGEAVPPSLLDRWSP--GRRLYNAYGPTE 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 372 --IGMALSNPLHGVRIpgSVGTPLPGVEVCIAtetlkdgarsytihaqgdESNTQvtPGLEGQEGELLVRGPSVFREYWN 449
Cdd:cd17653 244 ctISSTMTELLPGQPV--TIGKPIPNSTCYIL------------------DADLQ--PVPEGVVGEICISGVQVARGYLG 301
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 450 RPRETEDAFTPDGW------FKTGDTAAY-KDGVYWIKGRTSVDIiKNGGFKISALEVERHLLA-HPHITDVAVIgppdM 521
Cdd:cd17653 302 NPALTASKFVPDPFwpgsrmYRTGDYGRWtEDGGLEFLGREDNQV-KVRGFRINLEEIEEVVLQsQPEVTQAAAI----V 376
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2452540125 522 VWGQRVSAVVQlrkgEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQ 582
Cdd:cd17653 377 VNGRLVAFVTP----ETVDVDGLRSELAKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
70-580 |
1.64e-29 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 122.81 E-value: 1.64e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 70 DQNGEHTYRDLLSQSLRLSQEIcRALDCSSRdlkeERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQD 149
Cdd:PRK05857 37 DGTSALRYRELVAEVGGLAADL-RAQSVSRG----SRVLVISDNGPETYLSVLACAKLGAIAVMADGNLPIAAIERFCQI 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 150 SQSALVIAAEEyvGKITPSAEKLGVPVLPLLR---SCSDGSASHPAVEEGPLTTCSSWKNRGAMIIYTSGTTGRPKGVLS 226
Cdd:PRK05857 112 TDPAAALVAPG--SKMASSAVPEALHSIPVIAvdiAAVTRESEHSLDAASLAGNADQGSEDPLAMIFTSGTTGEPKAVLL 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 227 THENVQAVTTGLVEK---W-EWKKEDVILHVLPLHHVHGVINKLLCpLWVGATCVMLPEFNAQMvwKKLLSSQAprISVF 302
Cdd:PRK05857 190 ANRTFFAVPDILQKEglnWvTWVVGETTYSPLPATHIGGLWWILTC-LMHGGLCVTGGENTTSL--LEILTTNA--VATT 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 303 MAVPTIYAKLVeyYDKHFFQPQVqdfvrafcqENIRLMV-SGSAALPVPVleKWKSITGHTLLERYGMTEIG-MALSNPL 380
Cdd:PRK05857 265 CLVPTLLSKLV--SELKSANATV---------PSLRLVGyGGSRAIAADV--RFIEATGVRTAQVYGLSETGcTALCLPT 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 381 HGVRIP----GSVGTPLPGVEVCIATEtlkDGARSYTihAQGDESNTQvtpglegqeGELLVRGPSVFREYWNRPRETED 456
Cdd:PRK05857 332 DDGSIVkieaGAVGRPYPGVDVYLAAT---DGIGPTA--PGAGPSASF---------GTLWIKSPANMLGYWNNPERTAE 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 457 AFTpDGWFKTGDTAA-YKDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVS-AVVQLR 534
Cdd:PRK05857 398 VLI-DGWVNTGDLLErREDGFFYIKGRSS-EMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPDEEFGALVGlAVVASA 475
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 2452540125 535 KGEMLSVKDLKD--WAR-----DTMAPytiPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK05857 476 ELDESAARALKHtiAARfrresEPMAR---PSTIVIVTDIPRTQSGKVMRASL 525
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
59-580 |
2.57e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 124.50 E-value: 2.57e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKiaiidqngEHTYRDLLSQSLRLSQE-ICRALDcssrdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKK 137
Cdd:PRK12467 1592 ALVFGEQ--------ELTYGELNRRANRLAHRlIALGVG------PEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPE 1657
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 138 HPVTELEYVIQDSQSALVIAAEEYVGKItPSAEKLGVPVLPLLRSCSDG-SASHPAVEEGPlttcsswkNRGAMIIYTSG 216
Cdd:PRK12467 1658 YPRERLAYMIEDSGIELLLTQSHLQARL-PLPDGLRSLVLDQEDDWLEGySDSNPAVNLAP--------QNLAYVIYTSG 1728
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 217 TTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPlHHVHGVINKLLCPLWVGATCVMLP--------EFNAQMvw 288
Cdd:PRK12467 1729 STGRPKGAGNRHGALVNRLCATQEAYQLSAADVVLQFTS-FAFDVSVWELFWPLINGARLVIAPpgahrdpeQLIQLI-- 1805
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 kkllssQAPRISVFMAVPTIYAKLVEyYDKHFFQPQvqdfvrafcqeNIRLMVSGSAALPVPVLEKWKSITGHT-LLERY 367
Cdd:PRK12467 1806 ------ERQQVTTLHFVPSMLQQLLQ-MDEQVEHPL-----------SLRRVVCGGEALEVEALRPWLERLPDTgLFNLY 1867
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 368 GMTEIGM-------ALSNPLHGVRIPgsVGTPLPGVEVCIATETLkdgarsytihaqgdesntqvTPGLEGQEGELLVRG 440
Cdd:PRK12467 1868 GPTETAVdvthwtcRRKDLEGRDSVP--IGQPIANLSTYILDASL--------------------NPVPIGVAGELYLGG 1925
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 441 PSVFREYWNRPRETEDAFTPDGW-------FKTGDTAAYK-DGVYWIKGRtsVD-IIKNGGFKISALEVERHLLAHPHIT 511
Cdd:PRK12467 1926 VGLARGYLNRPALTAERFVADPFgtvgsrlYRTGDLARYRaDGVIEYLGR--IDhQVKIRGFRIELGEIEARLREQGGVR 2003
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 512 DVAVIgPPDMVWGQRVSAVVQLRKGEMLSVKD--------LKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK12467 2004 EAVVI-AQDGANGKQLVAYVVPTDPGLVDDDEaqvalraiLKNHLKASLPEYMVPAHLVFLARMPLTPNGKLDRKAL 2079
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
64-580 |
3.25e-29 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 120.65 E-value: 3.25e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEICRaldcssRDLKEERISFLCPNDASY-VVAQWASWMSGGIAVPLYKKHPVTE 142
Cdd:cd17650 2 DAIAVSDATRQLTYRELNERANQLARTLRG------LGVAPGSVVGVCADRSLDaIVGLLAVLKAGGAYVPIDPDYPAER 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 143 LEYVIQDSQSALVIAAEEYVgkitpsaeklgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTGRPK 222
Cdd:cd17650 76 LQYMLEDSGAKLLLTQPEDL----------------------------------------------AYVIYTSGTTGKPK 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 223 GVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPE---FNAQMVWKKLLSSqapRI 299
Cdd:cd17650 110 GVMVEHRNVAHAAHAWRREYELDSFPVRLLQMASFSFDVFAGDFARSLLNGGTLVICPDevkLDPAALYDLILKS---RI 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 300 SVFMAVPTIYAKLVEYYDKHFFQPqvqdfvrafcqENIRLMVSGSAALPVpvleKWKSitghTLLER----------YGM 369
Cdd:cd17650 187 TLMESTPALIRPVMAYVYRNGLDL-----------SAMRLLIVGSDGCKA----QDFK----TLAARfgqgmriinsYGV 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 370 TEIGMALSN-PLHGVRIPGS----VGTPLPGVEVCIATETLkdgarsytihaqgdesntQVTPglEGQEGELLVRGPSVF 444
Cdd:cd17650 248 TEATIDSTYyEEGRDPLGDSanvpIGRPLPNTAMYVLDERL------------------QPQP--VGVAGELYIGGAGVA 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 445 REYWNRPRETEDAFTPDGW------FKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIG 517
Cdd:cd17650 308 RGYLNRPELTAERFVENPFapgermYRTGDLARWRaDGNVELLGRVD-HQVKIRGFRIELGEIESQLARHPAIDEAVVAV 386
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2452540125 518 PPDMvwGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17650 387 REDK--GGEARLCAYVVAAATLNTAELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
64-582 |
3.40e-29 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 121.16 E-value: 3.40e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEIcraldcSSRDLKEER-------------ISFLcpndasyvvaqwASWMSGGI 130
Cdd:PRK04813 17 DFPAYDYLGEKLTYGQLKEDSDALAAFI------DSLKLPDKSpiivfghmspemlATFL------------GAVKAGHA 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 131 AVPLYKKHPVTELEYVIQDSQSALVIAAEEYvgkitpSAEKLGVPVLPLlRSCSDGSASHPAVEEgplttcSSW--KNRG 208
Cdd:PRK04813 79 YIPVDVSSPAERIEMIIEVAKPSLIIATEEL------PLEILGIPVITL-DELKDIFATGNPYDF------DHAvkGDDN 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENvqavttgLVEKWEWKKED--------------------VI-----------LHVLPlh 257
Cdd:PRK04813 146 YYIIFTSGTTGKPKGVQISHDN-------LVSFTNWMLEDfalpegpqflnqapysfdlsVMdlyptlasggtLVALP-- 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 258 hvHGVIN--KLL------CPL--WVgAT------CVMLPEFNAQMvwkkllssqaprisvfmavptiYAKLveyydKHFF 321
Cdd:PRK04813 217 --KDMTAnfKQLfetlpqLPInvWV-STpsfadmCLLDPSFNEEH----------------------LPNL-----THFL 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 322 qpqvqdfvraFCQEnirlmvsgsaALPVPVLEKwksitghtLLER---------YGMTEIGMALSnplhGVRIPGSV--- 389
Cdd:PRK04813 267 ----------FCGE----------ELPHKTAKK--------LLERfpsatiyntYGPTEATVAVT----SIEITDEMldq 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 390 GTPLP-GVEvciatetlKDGARSYTIHAQGdesntqvTPGLEGQEGELLVRGPSVFREYWNRPRETEDAF-TPDGW--FK 465
Cdd:PRK04813 315 YKRLPiGYA--------KPDSPLLIIDEEG-------TKLPDGEQGEIVISGPSVSKGYLNNPEKTAEAFfTFDGQpaYH 379
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 466 TGDTAAYKDGVYWIKGRtsVDI-IKNGGFKISALEVERHLLAHPHItDVAVIGP--PDMVWGQRVSAVV---QLRKGEML 539
Cdd:PRK04813 380 TGDAGYLEDGLLFYQGR--IDFqIKLNGYRIELEEIEQNLRQSSYV-ESAVVVPynKDHKVQYLIAYVVpkeEDFEREFE 456
|
570 580 590 600
....*....|....*....|....*....|....*....|...
gi 2452540125 540 SVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQ 582
Cdd:PRK04813 457 LTKAIKKELKERLMEYMIPRKFIYRDSLPLTPNGKIDRKALIE 499
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
209-580 |
4.37e-29 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 121.33 E-value: 4.37e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAqmvw 288
Cdd:PRK07867 155 FMLIFTSGTSGDPKAVRCTHRKVASAGVMLAQRFGLGPDDVCYVSMPLFHSNAVMAGWAVALAAGASIALRRKFSA---- 230
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 kkllSSQAPRISVFMAVPTIY-AKLVEYYdkhFFQPQVQDFvrafcQENIRLMVSGSAALPVPVlEKWKSITGHTLLERY 367
Cdd:PRK07867 231 ----SGFLPDVRRYGATYANYvGKPLSYV---LATPERPDD-----ADNPLRIVYGNEGAPGDI-ARFARRFGCVVVDGF 297
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 368 GMTEIGMALS----NPlhgvriPGSVGTPLPGVEVcIATETLKDGARSyTIHAQGDESNTQVTpglegqeGELL-VRGPS 442
Cdd:PRK07867 298 GSTEGGVAITrtpdTP------PGALGPLPPGVAI-VDPDTGTECPPA-EDADGRLLNADEAI-------GELVnTAGPG 362
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 443 VFREYWNRPrETEDAFTPDGWFKTGDTAaYKD--GVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPD 520
Cdd:PRK07867 363 GFEGYYNDP-EADAERMRGGVYWSGDLA-YRDadGYAYFAGRLG-DWMRVDGENLGTAPIERILLRYPDATEVAVYAVPD 439
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2452540125 521 MVWGQRVSAVVQLRKGEMLSVKDLKDW--ARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK07867 440 PVVGDQVMAALVLAPGAKFDPDAFAEFlaAQPDLGPKQWPSYVRVCAELPRTATFKVLKRQL 501
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
59-580 |
6.12e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 123.14 E-value: 6.12e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKiaiidqngEHTYRDLLSQSLRLSQEIcRALDCSSrdlkEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKH 138
Cdd:PRK12316 529 ALAFGEE--------TLDYAELNRRANRLAHAL-IERGVGP----DVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEY 595
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 139 PVTELEYVIQDSQSALVIAaEEYVGKITPSAEklGVPVLPLlrscSDGSASHPAVEEGPLTTCSSWKNRgAMIIYTSGTT 218
Cdd:PRK12316 596 PAERLAYMLEDSGVQLLLS-QSHLGRKLPLAA--GVQVLDL----DRPAAWLEGYSEENPGTELNPENL-AYVIYTSGST 667
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 219 GRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVInKLLCPLWVGATCVMLPEFNAQMVWKKLLSSQAPR 298
Cdd:PRK12316 668 GKPKGAGNRHRALSNRLCWMQQAYGLGVGDTVLQKTPFSFDVSVW-EFFWPLMSGARLVVAAPGDHRDPAKLVELINREG 746
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 299 ISVFMAVPTIyaklveyydkhfFQPQVQDFVRAFCqENIRLMVSGSAALPVPVLEK--WKSITGHtLLERYGMTE--IGM 374
Cdd:PRK12316 747 VDTLHFVPSM------------LQAFLQDEDVASC-TSLRRIVCSGEALPADAQEQvfAKLPQAG-LYNLYGPTEaaIDV 812
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 375 ALSNPLHGVRIPGSVGTPLPGVevciatetlkdgaRSYTIHAQGdesntQVTPglEGQEGELLVRGPSVFREYWNRPRET 454
Cdd:PRK12316 813 THWTCVEEGGDSVPIGRPIANL-------------ACYILDANL-----EPVP--VGVLGELYLAGRGLARGYHGRPGLT 872
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 455 EDAFTPDGW------FKTGDTAAYK-DGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGppdmVWGQRV 527
Cdd:PRK12316 873 AERFVPSPFvagermYRTGDLARYRaDGVIEYAGRID-HQVKLRGLRIELGEIEARLLEHPWVREAAVLA----VDGKQL 947
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 2452540125 528 SAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK12316 948 VGYVVLESEGGDWREALKAHLAASLPEYMVPAQWLALERLPLTPNGKLDRKAL 1000
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
64-580 |
6.28e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 122.96 E-value: 6.28e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEICraldcSSRDLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:PRK12467 527 ERPALVFGEQVLSYAELNRQANRLAHVLI-----AAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQDRL 601
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAAEEYVGKITPSAeklGVPVLPL---LRSCSDGSASHPAVEEGPLTTcsswknrgAMIIYTSGTTGR 220
Cdd:PRK12467 602 AYMLDDSGVRLLLTQSHLLAQLPVPA---GLRSLCLdepADLLCGYSGHNPEVALDPDNL--------AYVIYTSGSTGQ 670
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 221 PKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVInKLLCPLWVGATCVMLP--------EFNAQMVwkkll 292
Cdd:PRK12467 671 PKGVAISHGALANYVCVIAERLQLAADDSMLMVSTFAFDLGVT-ELFGALASGATLHLLPpdcardaeAFAALMA----- 744
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 293 ssqAPRISVFMAVPTiyaklveyydkhFFQPQVQDFVRAFCQENIRLMVSGSaALPVPVLEKWKSIT-GHTLLERYGMTE 371
Cdd:PRK12467 745 ---DQGVTVLKIVPS------------HLQALLQASRVALPRPQRALVCGGE-ALQVDLLARVRALGpGARLINHYGPTE 808
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 372 IGMALSN---PLHGVRIPGS-VGTPLPGVEVCIAtetlkdgarsytihaqgdesNTQVTPGLEGQEGELLVRGPSVFREY 447
Cdd:PRK12467 809 TTVGVSTyelSDEERDFGNVpIGQPLANLGLYIL--------------------DHYLNPVPVGVVGELYIGGAGLARGY 868
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 448 WNRPRETEDAFTPDGW-------FKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPP 519
Cdd:PRK12467 869 HRRPALTAERFVPDPFgadggrlYRTGDLARYrADGVIEYLGRMD-HQVKIRGFRIELGEIEARLLAQPGVREAVVLAQP 947
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2452540125 520 DMVWGQRVSAVVQ---LRKGEMLSVKD-LKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK12467 948 GDAGLQLVAYLVPaavADGAEHQATRDeLKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKAL 1012
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
118-580 |
6.57e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 123.14 E-value: 6.57e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 118 VVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALVIAAEEYVgkiTPSAEklGVPVLPLLRSCSDGSASHPAVEEGP 197
Cdd:PRK12316 3121 VVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLLLSQSHLR---LPLAQ--GVQVLDLDRGDENYAEANPAIRTMP 3195
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 198 lttcsswkNRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLhHVHGVINKLLCPLWVGATCV 277
Cdd:PRK12316 3196 --------ENLAYVIYTSGSTGKPKGVGIRHSALSNHLCWMQQAYGLGVGDRVLQFTTF-SFDVFVEELFWPLMSGARVV 3266
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 278 MLPEFNAQMVWKKLLSSQAPRISVFMAVPTIYAKLVEYYDKHFFQpqvqdfvrafcqeNIRLMVSGSAALPVPVLEKWks 357
Cdd:PRK12316 3267 LAGPEDWRDPALLVELINSEGVDVLHAYPSMLQAFLEEEDAHRCT-------------SLKRIVCGGEALPADLQQQV-- 3331
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 358 ITGHTLLERYGMTEigmALSNPLHGVRIPGSVGTPLPGVEVCIATETLKDGArsytihaqgdesntqVTPGLEGQEGELL 437
Cdd:PRK12316 3332 FAGLPLYNLYGPTE---ATITVTHWQCVEEGKDAVPIGRPIANRACYILDGS---------------LEPVPVGALGELY 3393
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 438 VRGPSVFREYWNRPRETEDAFTPDGW------FKTGDTAAYK-DGVYWIKGRTSVDiIKNGGFKISALEVERHLLAHPHI 510
Cdd:PRK12316 3394 LGGEGLARGYHNRPGLTAERFVPDPFvpgerlYRTGDLARYRaDGVIEYIGRVDHQ-VKIRGFRIELGEIEARLLEHPWV 3472
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 511 TDVAVIGppdmVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK12316 3473 REAVVLA----VDGRQLVAYVVPEDEAGDLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKAL 3538
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
206-584 |
8.02e-29 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 120.58 E-value: 8.02e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 206 NRGAMIIYTSGTTGRPKGVLSTHEN--VQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLwVGATCVML-PEF 282
Cdd:PRK07008 176 NQASSLCYTSGTTGNPKGALYSHRStvLHAYGAALPDAMGLSARDAVLPVVPMFHVNAWGLPYSAPL-TGAKLVLPgPDL 254
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 283 NAQMVWKKLlssQAPRISVFMAVPTIYAKLVEYydkhffqpqvqdfvrafCQEN------IRLMVSGSAALPVPVLEKWK 356
Cdd:PRK07008 255 DGKSLYELI---EAERVTFSAGVPTVWLGLLNH-----------------MREAglrfstLRRTVIGGSACPPAMIRTFE 314
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 357 SITGHTLLERYGMTEIgmalsNPLhgvripGSVGTPLpgvevcIATETLKDGARSYTIHAQGdesntQVTPGL------- 429
Cdd:PRK07008 315 DEYGVEVIHAWGMTEM-----SPL------GTLCKLK------WKHSQLPLDEQRKLLEKQG-----RVIYGVdmkivgd 372
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 430 EGQE--------GELLVRGPSVFREYWNRpretEDAFTPDGWFKTGDTAAY-KDGVYWIKGRtSVDIIKNGGFKISALEV 500
Cdd:PRK07008 373 DGRElpwdgkafGDLQVRGPWVIDRYFRG----DASPLVDGWFPTGDVATIdADGFMQITDR-SKDVIKSGGEWISSIDI 447
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 501 ERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK07008 448 ENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGAEVTREELLAFYEGKVAKWWIPDDVVFVDAIPHTATGKLQKLKL 527
|
....
gi 2452540125 581 LQRF 584
Cdd:PRK07008 528 REQF 531
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
172-580 |
4.20e-28 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 118.59 E-value: 4.20e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 172 LGVPVLPLLRS--------CSDGSASHPAVEEGPLTTcsswknrgAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWE 243
Cdd:PRK13388 116 LDLPGVRVLDVdtpayaelVAAAGALTPHREVDAMDP--------FMLIFTSGTTGAPKAVRCSHGRLAFAGRALTERFG 187
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 244 WKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAqmvwkkllssqapriSVFMAVptiyaklVEYYDKHFF-- 321
Cdd:PRK13388 188 LTRDDVCYVSMPLFHSNAVMAGWAPAVASGAAVALPAKFSA---------------SGFLDD-------VRRYGATYFny 245
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 322 ----------QPQVQD-----FVRAFcqenirlmvsGSAALPvpvlekwKSIT------GHTLLERYGMTEIGMALsnpl 380
Cdd:PRK13388 246 vgkplayilaTPERPDdadnpLRVAF----------GNEASP-------RDIAefsrrfGCQVEDGYGSSEGAVIV---- 304
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 381 hgVRIP----GSVGTPLPGVEVcIATETLKDGARsytihAQGDESNTQVTPglEGQEGELLVR-GPSVFREYWNRPRETE 455
Cdd:PRK13388 305 --VREPgtppGSIGRGAPGVAI-YNPETLTECAV-----ARFDAHGALLNA--DEAIGELVNTaGAGFFEGYYNNPEATA 374
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 456 DAFTpDGWFKTGDTAaYKDGVYWI--KGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQL 533
Cdd:PRK13388 375 ERMR-HGMYWSGDLA-YRDADGWIyfAGRTA-DWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVL 451
|
410 420 430 440
....*....|....*....|....*....|....*....|....*....
gi 2452540125 534 RKGEMLSVKDLKDW--ARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK13388 452 RDGATFDPDAFAAFlaAQPDLGTKAWPRYVRIAADLPSTATNKVLKREL 500
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
63-575 |
6.34e-28 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 118.43 E-value: 6.34e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 63 GDKIAII------DQNGEHTYRDLLsqslrlsQEICR---ALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGI 130
Cdd:cd05966 67 GDKVAIIwegdepDQSRTITYRELL-------REVCRfanVL----KSLgvkKGDRVAIYMPMIPELVIAMLACARIGAV 135
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 131 avplykkHPVT-------ELEYVIQDSQSALVIAAEEYV--GKI-------------TPSAEK------LGVPV------ 176
Cdd:cd05966 136 -------HSVVfagfsaeSLADRINDAQCKLVITADGGYrgGKViplkeivdealekCPSVEKvlvvkrTGGEVpmtegr 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 177 -LPLLRSCSDGSASHPAVE---EGPLttcsswknrgaMIIYTSGTTGRPKGVLSTHE--NVQAVTTGlveKW--EWKKED 248
Cdd:cd05966 209 dLWWHDLMAKQSPECEPEWmdsEDPL-----------FILYTSGSTGKPKGVVHTTGgyLLYAATTF---KYvfDYHPDD 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 249 V---------------IlhvlplhhVHGvinkllcPLWVGATCVML------PEFNA--QMVWKKllssqapRISVFMAV 305
Cdd:cd05966 275 IywctadigwitghsyI--------VYG-------PLANGATTVMFegtptyPDPGRywDIVEKH-------KVTIFYTA 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 306 PTIYAKLVEYYDkhffqpqvqDFVRAFCQENIRLMvsGSAALPV-PvlEKWK---SITGH---TLLERYGMTEIGMALSN 378
Cdd:cd05966 333 PTAIRALMKFGD---------EWVKKHDLSSLRVL--GSVGEPInP--EAWMwyyEVIGKercPIVDTWWQTETGGIMIT 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 379 PLHGVR--IPGSVGTPLPGVEVCIATEtlkdgarsytihaqgdesNTQVTPGleGQEGELLVRG--PSVFREYWNRPRET 454
Cdd:cd05966 400 PLPGATplKPGSATRPFFGIEPAILDE------------------EGNEVEG--EVEGYLVIKRpwPGMARTIYGDHERY 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 455 EDAFTPD--GWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVV 531
Cdd:cd05966 460 EDTYFSKfpGYYFTGDGARRdEDGYYWITGRVD-DVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFV 538
|
570 580 590 600
....*....|....*....|....*....|....*....|....*..
gi 2452540125 532 QLRKGEMLS---VKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKV 575
Cdd:cd05966 539 TLKDGEEPSdelRKELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKI 585
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
64-580 |
7.09e-28 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 116.77 E-value: 7.09e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLsqeicrALDCSSRDLKEERISFLC-PNDASYVVAQWASWMSGGIAVPLYKKHPVTE 142
Cdd:cd17644 15 DAVAVVFEDQQLTYEELNTKANQL------AHYLQSLGVKSESLVGICvERSLEMIIGLLAILKAGGAYVPLDPNYPQER 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 143 LEYVIQDSQSALVIAAEEYVgkitpsaeklgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTGRPK 222
Cdd:cd17644 89 LTYILEDAQISVLLTQPENL----------------------------------------------AYVIYTSGSTGKPK 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 223 GVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLhhVHGVINKLLCPLWV-GATCVMLPE---FNAQMVWKKLlssQAPR 298
Cdd:cd17644 123 GVMIEHQSLVNLSHGLIKEYGITSSDRVLQFASI--AFDVAAEEIYVTLLsGATLVLRPEemrSSLEDFVQYI---QQWQ 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 299 ISVFmAVPTIYAKLVeyydkhffqpqVQDFVRAFCQ--ENIRLMVSGSAALPVPVLEKWKSITGH--TLLERYGMTEIGM 374
Cdd:cd17644 198 LTVL-SLPPAYWHLL-----------VLELLLSTIDlpSSLRLVIVGGEAVQPELVRQWQKNVGNfiQLINVYGPTEATI 265
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 375 A-----LSNPLHGVRIPGSVGTPLPGVEVCIATETLKdgarsytihaqgdesntQVTPGLegqEGELLVRGPSVFREYWN 449
Cdd:cd17644 266 AatvcrLTQLTERNITSVPIGRPIANTQVYILDENLQ-----------------PVPVGV---PGELHIGGVGLARGYLN 325
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 450 RPRETEDAFTPDGW--------FKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPD 520
Cdd:cd17644 326 RPELTAEKFISHPFnsseserlYKTGDLARYlPDGNIEYLGRID-NQVKIRGFRIELGEIEAVLSQHNDVKTAVVIVRED 404
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 521 MVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17644 405 QPGNKRLVAYIVPHYEESPSTVELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
148-580 |
8.53e-28 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 118.60 E-value: 8.53e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 148 QDSQSALVIAAEEYVGKITPSAeklgvpVLPLLRSCSDGSASHPAVEEgPLTtcsswKNRGAMIIYTSGTTGRPKGVLST 227
Cdd:PRK06060 99 RNTEPALVVTSDALRDRFQPSR------VAEAAELMSEAARVAPGGYE-PMG-----GDALAYATYTSGTTGPPKAAIHR 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 228 HENVQAVTTGLVEK-WEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWKKLLSSQAPriSVFMAVP 306
Cdd:PRK06060 167 HADPLTFVDAMCRKaLRLTPEDTGLCSARMYFAYGLGNSVWFPLATGGSAVINSAPVTPEAAAILSARFGP--SVLYGVP 244
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 307 TIYAKLVEYYDKHFFQpqvqdfvrafcqeNIRLMVSGSAALPVPVLEKWKSITGHT-LLERYGMTEIGMA-LSNPLHGVR 384
Cdd:PRK06060 245 NFFARVIDSCSPDSFR-------------SLRCVVSAGEALELGLAERLMEFFGGIpILDGIGSTEVGQTfVSNRVDEWR 311
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 385 iPGSVGTPLPGVEVCIATetlKDGArsytihaqgdesntqvTPGlEGQEGELLVRGPSVFREYWNRPretEDAFTPDGWF 464
Cdd:PRK06060 312 -LGTLGRVLPPYEIRVVA---PDGT----------------TAG-PGVEGDLWVRGPAIAKGYWNRP---DSPVANEGWL 367
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 465 KTGDTAAYkDGVYWIK-GRTSVDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKD 543
Cdd:PRK06060 368 DTRDRVCI-DSDGWVTyRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATIDGSV 446
|
410 420 430 440
....*....|....*....|....*....|....*....|
gi 2452540125 544 LKDWAR---DTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK06060 447 MRDLHRgllNRLSAFKVPHRFAVVDRLPRTPNGKLVRGAL 486
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
213-580 |
4.02e-27 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 115.71 E-value: 4.02e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 213 YTSGTTGRPKGVLSTHEN--VQAVTTGLVekWEWKKEDVILHVLPLHHVHGvinklLCPLWVGA----TCVMLPEFNAQM 286
Cdd:PLN02479 202 YTSGTTASPKGVVLHHRGayLMALSNALI--WGMNEGAVYLWTLPMFHCNG-----WCFTWTLAalcgTNICLRQVTAKA 274
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 287 VWKKLLSSQaprISVFMAVPTIYAKLVEYYDKHFFQPqvqdfvrafCQENIRLMVSGSAalPVPVLEKWKSITGHTLLER 366
Cdd:PLN02479 275 IYSAIANYG---VTHFCAAPVVLNTIVNAPKSETILP---------LPRVVHVMTAGAA--PPPSVLFAMSEKGFRVTHT 340
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 367 YGMTE-------------------IGMALSNPLHGVRIPGsvgtpLPGVEVcIATETLKdgarsyTIHAQGDESntqvtp 427
Cdd:PLN02479 341 YGLSEtygpstvcawkpewdslppEEQARLNARQGVRYIG-----LEGLDV-VDTKTMK------PVPADGKTM------ 402
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 428 glegqeGELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTAA-YKDGVYWIKGRtSVDIIKNGGFKISALEVERHLLA 506
Cdd:PLN02479 403 ------GEIVMRGNMVMKGYLKNPKANEEAFA-NGWFHSGDLGVkHPDGYIEIKDR-SKDIIISGGENISSLEVENVVYT 474
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2452540125 507 HPHITDVAVIGPPDMVWGQRVSAVVQLRKG-----EMLSVKDLKDWARDTMAPYTIPTElIVVEEIPRNQMGKVNKKEL 580
Cdd:PLN02479 475 HPAVLEASVVARPDERWGESPCAFVTLKPGvdksdEAALAEDIMKFCRERLPAYWVPKS-VVFGPLPKTATGKIQKHVL 552
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
209-578 |
6.76e-27 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 116.18 E-value: 6.76e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLP-----EFN 283
Cdd:PRK08633 785 ATIIFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVILSSLPFFHSFGLTVTLWLPLLEGIKVVYHPdptdaLGI 864
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 284 AQMVWKKllssqapRISVFMAVPT---IYAKlveyydkhffQPQVQ--DFvrafcqENIRLMVSGSAALPVPVLEKWKSI 358
Cdd:PRK08633 865 AKLVAKH-------RATILLGTPTflrLYLR----------NKKLHplMF------ASLRLVVAGAEKLKPEVADAFEEK 921
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 359 TGHTLLERYGMTE-----------IGMALSNPLHGVRiPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTP 427
Cdd:PRK08633 922 FGIRILEGYGATEtspvasvnlpdVLAADFKRQTGSK-EGSVGMPLPGVAVRIV-----------------DPETFEELP 983
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 428 glEGQEGELLVRGPSVFREYWNRPRETEDAFT---PDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKIS--ALEVE 501
Cdd:PRK08633 984 --PGEDGLILIGGPQVMKGYLGDPEKTAEVIKdidGIGWYVTGDKGHLdEDGFLTITDRYS-RFAKIGGEMVPlgAVEEE 1060
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 502 RHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLkdWARDTMAPYTIPTELIVVEEIPRNQMGKVNKK 578
Cdd:PRK08633 1061 LAKALGGEEVVFAVTAVPDEKKGEKLVVLHTCGAEDVEELKRA--IKESGLPNLWKPSRYFKVEALPLLGSGKLDLK 1135
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
59-580 |
6.79e-27 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 116.80 E-value: 6.79e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKiaiidqngEHTYRDLLSQSLRLSQE-ICRALDCssrdlkEERISFLCPNDASYVVAQWASWMSGGIAVPLYKK 137
Cdd:PRK12467 3113 ALVFGDQ--------QLSYAELNRRANRLAHRlIAIGVGP------DVLVGVAVERSVEMIVALLAVLKAGGAYVPLDPE 3178
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 138 HPVTELEYVIQDSQSALVIAAEEYVgkitpsaEKLGVPVLpLLRSCSDGSASHPAVEEGPLTTCSSwkNRGAMIIYTSGT 217
Cdd:PRK12467 3179 YPRERLAYMIEDSGVKLLLTQAHLL-------EQLPAPAG-DTALTLDRLDLNGYSENNPSTRVMG--ENLAYVIYTSGS 3248
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 218 TGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLhHVHGVINKLLCPLWVGATCVMLP--EFNAQMVWKKLLssq 295
Cdd:PRK12467 3249 TGKPKGVGVRHGALANHLCWIAEAYELDANDRVLLFMSF-SFDGAQERFLWTLICGGCLVVRDndLWDPEELWQAIH--- 3324
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 296 APRISVFMAVPTIYAKLVEYYDkhffqpqVQDFVRafcqenIRLMVSGSAALPVPVLEKWKS-ITGHTLLERYGMTEigm 374
Cdd:PRK12467 3325 AHRISIACFPPAYLQQFAEDAG-------GADCAS------LDIYVFGGEAVPPAAFEQVKRkLKPRGLTNGYGPTE--- 3388
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 375 ALSNPLH----GVRIPGSVGTPLpGVEVCIATETLKDGarsytihaqgdesntQVTPGLEGQEGELLVRGPSVFREYWNR 450
Cdd:PRK12467 3389 AVVTVTLwkcgGDAVCEAPYAPI-GRPVAGRSIYVLDG---------------QLNPVPVGVAGELYIGGVGLARGYHQR 3452
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 451 PRETEDAFTPDGW-------FKTGDTAAYK-DGVYWIKGRtsVD-IIKNGGFKISALEVERHLLAHPHITDVAVIGPPDM 521
Cdd:PRK12467 3453 PSLTAERFVADPFsgsggrlYRTGDLARYRaDGVIEYLGR--IDhQVKIRGFRIELGEIEARLLQHPSVREAVVLARDGA 3530
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 522 VWGQRVSAVV-QLRKGEMLSVkdLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK12467 3531 GGKQLVAYVVpADPQGDWRET--LRDHLAASLPDYMVPAQLLVLAAMPLGPNGKVDRKAL 3588
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
74-584 |
1.88e-25 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 110.59 E-value: 1.88e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 74 EHTYRDLLSQSLRLsqeicrALDCSSRDLKE-ERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQS 152
Cdd:cd17641 11 EFTWADYADRVRAF------ALGLLALGVGRgDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGA 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 153 ALVIAA-EEYVGKITPSAEKlgVPVL----------------PLLRSCSD-------GSASHPAVEEGPLttcsswkNRG 208
Cdd:cd17641 85 RVVIAEdEEQVDKLLEIADR--IPSVryviycdprgmrkyddPRLISFEDvvalgraLDRRDPGLYEREV-------AAG 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 -----AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGaTCVMLPE-- 281
Cdd:cd17641 156 kgedvAVLCTTSGTTGKPKLAMLSHGNFLGHCAAYLAADPLGPGDEYVSVLPLPWIGEQMYSVGQALVCG-FIVNFPEep 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 282 ---------------FNAQMVWKKLLS------SQAPRISVFM-------------------AVPTIYAKLVEYYDKHFF 321
Cdd:cd17641 235 etmmedlreigptfvLLPPRVWEGIAAdvrarmMDATPFKRFMfelgmklglraldrgkrgrPVSLWLRLASWLADALLF 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 322 QPQVQDFVRAFCqeniRLMVSGSAALPVPVLEKWKSItGHTLLERYGMTEI-GMALSNPLHGVRiPGSVGTPLPGVEVCI 400
Cdd:cd17641 315 RPLRDRLGFSRL----RSAATGGAALGPDTFRFFHAI-GVPLKQLYGQTELaGAYTVHRDGDVD-PDTVGVPFPGTEVRI 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 401 AtetlkdgarsytihaqgdesntqvtpglegQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAYK-DGVYWI 479
Cdd:cd17641 389 D------------------------------EVGEILVRSPGVFVGYYKNPEATAEDFDEDGWLHTGDAGYFKeNGHLVV 438
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 480 KGRTSvDIIK-NGGFKISALEVERHLLAHPHITDVAVIG---PpdmvwgqRVSAVVQLRkgemlsVKDLKDWARDTMAPY 555
Cdd:cd17641 439 IDRAK-DVGTtSDGTRFSPQFIENKLKFSPYIAEAVVLGagrP-------YLTAFICID------YAIVGKWAEQRGIAF 504
|
570 580 590
....*....|....*....|....*....|....*.
gi 2452540125 556 TIPTELIV---VEEIPRNQMGKVNK----KELLQRF 584
Cdd:cd17641 505 TTYTDLASrpeVYELIRKEVEKVNAslpeAQRIRRF 540
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
64-580 |
7.68e-25 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 107.95 E-value: 7.68e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSqeicRALDCSSRdLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:cd17656 3 DAVAVVFENQKLTYRELNERSNQLA----RFLREKGV-KKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERR 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAAEEyvgkITPSAEKLGVPVLPLLRSCSDGSASHpaveegplTTCSSWKNRGAMIIYTSGTTGRPKG 223
Cdd:cd17656 78 IYIMLDSGVRVVLTQRH----LKSKLSFNKSTILLEDPSISQEDTSN--------IDYINNSDDLLYIIYTSGTTGKPKG 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 224 VLSTHENVQAVTTGLVEKWEWKKEDVILH-VLPLHHV--HGVINKLLcplwVGATCVMLPEfNAQMVWKKL--LSSQAPR 298
Cdd:cd17656 146 VQLEHKNMVNLLHFEREKTNINFSDKVLQfATCSFDVcyQEIFSTLL----SGGTLYIIRE-ETKRDVEQLfdLVKRHNI 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 299 ISVFMavPTIYAKLV--EYYDKHFFQPQVQDFVRAFCQenirLMVSGsaalpvPVLEKWKSiTGHTLLERYGMTEIGMAL 376
Cdd:cd17656 221 EVVFL--PVAFLKFIfsEREFINRFPTCVKHIITAGEQ----LVITN------EFKEMLHE-HNVHLHNHYGPSETHVVT 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 377 SNPL-HGVRIP--GSVGTPLPGVEVCIAtetlkdgarsytihaqgdESNTQVTPglEGQEGELLVRGPSVFREYWNRPRE 453
Cdd:cd17656 288 TYTInPEAEIPelPPIGKPISNTWIYIL------------------DQEQQLQP--QGIVGELYISGASVARGYLNRQEL 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 454 TEDAFTPDGW------FKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQR 526
Cdd:cd17656 348 TAEKFFPDPFdpnermYRTGDLARYlPDGNIEFLGRAD-HQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKY 426
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 2452540125 527 VSA-VVQLRKgemLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17656 427 LCAyFVMEQE---LNISQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
209-475 |
1.01e-24 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 108.65 E-value: 1.01e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCpLWVGATCvmlpEFNAQMVW 288
Cdd:PLN02736 224 ATICYTSGTTGTPKGVVLTHGNLIANVAGSSLSTKFYPSDVHISYLPLAHIYERVNQIVM-LHYGVAV----GFYQGDNL 298
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 KKLLSSQAPRISVFMAVP----TIYAKL----------------VEYYDKH-----------FFQPQVQDFVRAFCQENI 337
Cdd:PLN02736 299 KLMDDLAALRPTIFCSVPrlynRIYDGItnavkesgglkerlfnAAYNAKKqalengknpspMWDRLVFNKIKAKLGGRV 378
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 338 RLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMALSNPLHGVRIPGSVGTPLPGVEVciateTLKDGAR-SYTiha 416
Cdd:PLN02736 379 RFMSSGASPLSPDVMEFLRICFGGRVLEGYGMTETSCVISGMDEGDNLSGHVGSPNPACEV-----KLVDVPEmNYT--- 450
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*....
gi 2452540125 417 qgdeSNTQVTPglegqEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAYKDG 475
Cdd:PLN02736 451 ----SEDQPYP-----RGEICVRGPIIFKGYYKDEVQTREVIDEDGWLHTGDIGLWLPG 500
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
57-580 |
1.11e-24 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 109.87 E-value: 1.11e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 57 TKALAFGDKIAIIdQNGEH-TYRDLLSQSLRLSqeicRALdcSSRDLKEE-RISFLCPNDASYVVAQWASWMSGGIAVPL 134
Cdd:PRK05691 2196 AQAARTPQAPALT-FAGQTlSYAELDARANRLA----RAL--RERGVGPQvRVGLALERSLEMVVGLLAILKAGGAYVPL 2268
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 135 YKKHPVTELEYVIQDSQSALVIAAEEYVgkitpsaEKLGVPVLPLLRSC-SDGSASHPAVEEGPLTTCSSWKNRgAMIIY 213
Cdd:PRK05691 2269 DPEYPLERLHYMIEDSGIGLLLSDRALF-------EALGELPAGVARWClEDDAAALAAYSDAPLPFLSLPQHQ-AYLIY 2340
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 214 TSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLhHVHGVINKLLCPLWVGATCVMlpefNAQMVWK---- 289
Cdd:PRK05691 2341 TSGSTGKPKGVVVSHGEIAMHCQAVIERFGMRADDCELHFYSI-NFDAASERLLVPLLCGARVVL----RAQGQWGaeei 2415
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 290 -KLLSSQapRISVFMAVPTIYAKLVEYYDKhffQPQvqdfvrafcQENIRLMVSGSAALpvpVLEKWKSITG----HTLL 364
Cdd:PRK05691 2416 cQLIREQ--QVSILGFTPSYGSQLAQWLAG---QGE---------QLPVRMCITGGEAL---TGEHLQRIRQafapQLFF 2478
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 365 ERYGMTEigmalsnplhgvripgSVGTPLpgveVCIATETLKDGARSYTI-HAQGDES----NTQVTPGLEGQEGELLVR 439
Cdd:PRK05691 2479 NAYGPTE----------------TVVMPL----ACLAPEQLEEGAASVPIgRVVGARVayilDADLALVPQGATGELYVG 2538
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 440 GPSVFREYWNRP-----RETEDAFTPDG--WFKTGDTAAYK-DGVYWIKGRTSVDiIKNGGFKISALEVERHLLAHPHIT 511
Cdd:PRK05691 2539 GAGLAQGYHDRPgltaeRFVADPFAADGgrLYRTGDLVRLRaDGLVEYVGRIDHQ-VKIRGFRIELGEIESRLLEHPAVR 2617
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2452540125 512 DVAVI-----GPPDMVwGQRVSAVVQLRKGEMLSVKD-LKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK05691 2618 EAVVLaldtpSGKQLA-GYLVSAVAGQDDEAQAALREaLKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRAL 2691
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
58-580 |
3.04e-24 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 108.33 E-value: 3.04e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 58 KALAFGDKIAIIDQNGEHTYRDLLSQSLRLSQEIcraldcssRDL---KEERISFLCPNDASYVVAQWASWMSGGIAVPL 134
Cdd:PRK05691 1140 QARQTPERIALVWDGGSLDYAELHAQANRLAHYL--------RDKgvgPDVCVAIAAERSPQLLVGLLAILKAGGAYVPL 1211
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 135 YKKHPVTELEYVIQDSQSALVIAAEEYVGKItPSAEklGVPVLPLLRSCSDGSASHP---AVEEGPLttcsswknrgAMI 211
Cdd:PRK05691 1212 DPDYPAERLAYMLADSGVELLLTQSHLLERL-PQAE--GVSAIALDSLHLDSWPSQApglHLHGDNL----------AYV 1278
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 212 IYTSGTTGRPKGVLSTHenvqavtTGLVEKWEWKK-------EDVILHVLPLHHVHGVINKLLcPLWVGATCVML----- 279
Cdd:PRK05691 1279 IYTSGSTGQPKGVGNTH-------AALAERLQWMQatyalddSDVLMQKAPISFDVSVWECFW-PLITGCRLVLAgpgeh 1350
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 280 --PEFNAQMVwkkllssQAPRISVFMAVPTIYAKLVEyydkhffQPQVQDfvrafCQeNIRLMVSGSAALPVP----VLE 353
Cdd:PRK05691 1351 rdPQRIAELV-------QQYGVTTLHFVPPLLQLFID-------EPLAAA-----CT-SLRRLFSGGEALPAElrnrVLQ 1410
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 354 KWKSITGHTlleRYGMTEIGMalsNPLH-------GVRIPgsVGTPLPGVeVCiatETLkdgarsytihaqgDESNTQVT 426
Cdd:PRK05691 1411 RLPQVQLHN---RYGPTETAI---NVTHwqcqaedGERSP--IGRPLGNV-LC---RVL-------------DAELNLLP 1465
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 427 PGLegqEGELLVRGPSVFREYWNRPRETEDAFTPDGW-------FKTGDTAAYK-DGVYWIKGRTSVDiIKNGGFKISAL 498
Cdd:PRK05691 1466 PGV---AGELCIGGAGLARGYLGRPALTAERFVPDPLgedgarlYRTGDRARWNaDGALEYLGRLDQQ-VKLRGFRVEPE 1541
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 499 EVERHLLAHPHITDVAVIgPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKK 578
Cdd:PRK05691 1542 EIQARLLAQPGVAQAAVL-VREGAAGAQLVGYYTGEAGQEAEAERLKAALAAELPEYMVPAQLIRLDQMPLGPSGKLDRR 1620
|
..
gi 2452540125 579 EL 580
Cdd:PRK05691 1621 AL 1622
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
208-580 |
3.13e-24 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 105.50 E-value: 3.13e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 208 GAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMV 287
Cdd:PRK08308 103 PSLLQYSSGTTGEPKLIRRSWTEIDREIEAYNEALNCEQDETPIVACPVTHSYGLICGVLAALTRGSKPVIITNKNPKFA 182
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 288 WKKLlssQAPRISVFMAVPTIYAKLVEyydkhfFQPQVQDFVRAfcqenirlMVSGsAALPVPVLEKWKSITGHtLLERY 367
Cdd:PRK08308 183 LNIL---RNTPQHILYAVPLMLHILGR------LLPGTFQFHAV--------MTSG-TPLPEAWFYKLRERTTY-MMQQY 243
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 368 GMTEIG-MALSnplHGVRIPGSVGTPLPGVEVCIATEtlkdgarsytihaqgdesntqvtpglEGQEGELLVrgpsvfre 446
Cdd:PRK08308 244 GCSEAGcVSIC---PDMKSHLDLGNPLPHVSVSAGSD--------------------------ENAPEEIVV-------- 286
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 447 yWNRPRETedaFTPDGWFKTGDtaaykdGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQR 526
Cdd:PRK08308 287 -KMGDKEI---FTKDLGYKSER------GTLHFMGRMD-DVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGER 355
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....
gi 2452540125 527 VSAVVQLRkgEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK08308 356 VKAKVISH--EEIDPVQLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
168-583 |
4.84e-24 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 104.96 E-value: 4.84e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 168 SAEKLGVPVLPL--------LRSCSD-GSASHPAVEEgplttcSSWKNRGAMIIYTSGTTGRPKGVLSTHENVQAvttGL 238
Cdd:cd05974 44 AAMKLGAVVIPAttlltpddLRDRVDrGGAVYAAVDE------NTHADDPMLLYFTSGTTSKPKLVEHTHRSYPV---GH 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 239 VEKWEW---KKEDVILHVLPLHHVHGVINKLLCPLWVGATCVML--PEFNAQMVWKKLLSSqapRISVFMAVPTIYAKLV 313
Cdd:cd05974 115 LSTMYWiglKPGDVHWNISSPGWAKHAWSCFFAPWNAGATVFLFnyARFDAKRVLAALVRY---GVTTLCAPPTVWRMLI 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 314 EyydkhffqpqvQDFVRAfcQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMALSNPLHGVRIPGSVGTPL 393
Cdd:cd05974 192 Q-----------QDLASF--DVKLREVVGAGEPLNPEVIEQVRRAWGLTIRDGYGQTETTALVGNSPGQPVKAGSMGRPL 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 394 PGVEVciateTLKDGArsytihaqgdesntqvtpGLEGQEGELLV-----RGPSVFREYWNRPRETEDAFTpDGWFKTGD 468
Cdd:cd05974 259 PGYRV-----ALLDPD------------------GAPATEGEVALdlgdtRPVGLMKGYAGDPDKTAHAMR-GGYYRTGD 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 469 TAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLS---VKDL 544
Cdd:cd05974 315 IAMRdEDGYLTYVGRAD-DVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPSpetALEI 393
|
410 420 430
....*....|....*....|....*....|....*....
gi 2452540125 545 KDWARDTMAPYTIPTELIVVeEIPRNQMGKVNKKELLQR 583
Cdd:cd05974 394 FRFSRERLAPYKRIRRLEFA-ELPKTISGKIRRVELRRR 431
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
212-582 |
1.28e-23 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 103.92 E-value: 1.28e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 212 IYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILhVLPLHHVHGVInKLLCPLWVGATCVMLPefnaqmvWKKL 291
Cdd:PRK07445 126 IPTGGSSGQIRFAIHTWETLTASVQGFQRYFQLQQVNSFC-VLPLYHVSGLM-QFMRSFLTGGKLVILP-------YKRL 196
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 292 LSSQAPRISV---FMA-VPTiyaKLveyydKHFFQPQVQdFVRAFcqeniRLMVSGSAalpvPVlekWKSitghtLLER- 366
Cdd:PRK07445 197 KSGQELPPNPsdfFLSlVPT---QL-----QRLLQLRPQ-WLAQF-----RTILLGGA----PA---WPS-----LLEQa 250
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 367 ----------YGMTEIG--MALSNP---LHGVRipgSVGTPLPgvevciatetlkdgarsytiHAQGDESNTQVtpgleg 431
Cdd:PRK07445 251 rqlqlrlaptYGMTETAsqIATLKPddfLAGNN---SSGQVLP--------------------HAQITIPANQT------ 301
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 432 qeGELLVRGPSVFREYWnrPreteDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSVDIIkNGGFKISALEVERHLLAHPHI 510
Cdd:PRK07445 302 --GNITIQAQSLALGYY--P----QILDSQGIFETDDLGYLdAQGYLHILGRNSQKII-TGGENVYPAEVEAAILATGLV 372
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2452540125 511 TDVAVIGPPDMVWGQRVSAVVQLRKGEmLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKELLQ 582
Cdd:PRK07445 373 QDVCVLGLPDPHWGEVVTAIYVPKDPS-ISLEELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQ 443
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
63-574 |
4.46e-23 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 103.68 E-value: 4.46e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 63 GDKIAII---DQNGEH---TYRDLLsqslrlsQEICR---ALdcssRDL---KEERISFLCPNDASYVVAQWASWMSGGI 130
Cdd:PRK00174 81 GDKVAIIwegDDPGDSrkiTYRELH-------REVCRfanAL----KSLgvkKGDRVAIYMPMIPEAAVAMLACARIGAV 149
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 131 avplykkHPVteleyV------------IQDSQSALVIAAEEYV--GKI-------------TPSAEKlgvpVLPLLRSC 183
Cdd:PRK00174 150 -------HSV-----VfggfsaealadrIIDAGAKLVITADEGVrgGKPiplkanvdealanCPSVEK----VIVVRRTG 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 184 SDG-----------------SASHPAVE---EGPLttcsswknrgaMIIYTSGTTGRPKGVLSTHE--NVQAVTTglvEK 241
Cdd:PRK00174 214 GDVdwvegrdlwwhelvagaSDECEPEPmdaEDPL-----------FILYTSGSTGKPKGVLHTTGgyLVYAAMT---MK 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 242 W--EWKKEDV---------------IlhvlplhhVHGvinkllcPLWVGATCVML---PEF-NAQMVWKKLlssQAPRIS 300
Cdd:PRK00174 280 YvfDYKDGDVywctadvgwvtghsyI--------VYG-------PLANGATTLMFegvPNYpDPGRFWEVI---DKHKVT 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 301 VFMAVPTIYAKLVEYYDkhffqpqvqDFVRAFCQENIRLMvsGSAALPV-PvlEKWK---SITGHtllER------YGMT 370
Cdd:PRK00174 342 IFYTAPTAIRALMKEGD---------EHPKKYDLSSLRLL--GSVGEPInP--EAWEwyyKVVGG---ERcpivdtWWQT 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 371 EIGMALSNPLHGVR--IPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGlegQEGELLVRG--PSVFRE 446
Cdd:PRK00174 406 ETGGIMITPLPGATplKPGSATRPLPGIQPAVV-----------------DEEGNPLEGG---EGGNLVIKDpwPGMMRT 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 447 YWNRPRETEDAFTPD--GWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVW 523
Cdd:PRK00174 466 IYGDHERFVKTYFSTfkGMYFTGDGARRdEDGYYWITGRVD-DVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIK 544
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....
gi 2452540125 524 GQRVSAVVQLRKGEMLS---VKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGK 574
Cdd:PRK00174 545 GQGIYAFVTLKGGEEPSdelRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGK 598
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
65-584 |
6.04e-23 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 102.57 E-value: 6.04e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 65 KIAIIDQNGEH---TYRDLLSQSLrlsqEICRALDCSSRDLKEErISFLCPNDASYVVAQWASWMSGGIAVPLYkkhpvt 141
Cdd:cd05908 3 GIIFILGDKKEkfvSYRHLREEAL----GYLGALQELGIKPGQE-VVFQITHNNKFLYLFWACLLGGMIAVPVS------ 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 142 eleyviqdsqsalVIAAEEYVGKITPSAEKLGVPVLpllrscsdgsashpaveegpLTTCSSW---KNRGAMIIYTSGTT 218
Cdd:cd05908 72 -------------IGSNEEHKLKLNKVWNTLKNPYL--------------------ITEEEVLcelADELAFIQFSSGST 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 219 GRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLP--EFNAQ-MVWKKLLSSQ 295
Cdd:cd05908 119 GDPKGVMLTHENLVHNMFAILNSTEWKTKDRILSWMPLTHDMGLIAFHLAPLIAGMNQYLMPtrLFIRRpILWLKKASEH 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 296 APRIsvfMAVPTIYAKLVeyydKHFFQPQVQDFVRAfcqENIRLMVSGSAALPVPVLEKW-KSITGHTL-----LERYGM 369
Cdd:cd05908 199 KATI---VSSPNFGYKYF----LKTLKPEKANDWDL---SSIRMILNGAEPIDYELCHEFlDHMSKYGLkrnaiLPVYGL 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 370 TEIGMALSNP----------------LHGVRIPG------------SVGTPLPGVEVCIAtetlkdgarsytihaqgDES 421
Cdd:cd05908 269 AEASVGASLPkaqspfktitlgrrhvTHGEPEPEvdkkdsecltfvEVGKPIDETDIRIC-----------------DED 331
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 422 NtQVTPglEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAYKDGVYWIKGRTSVDIIKNG------GFKI 495
Cdd:cd05908 332 N-KILP--DGYIGHIQIRGKNVTPGYYNNPEATAKVFTDDGWLKTGDLGFIRNGRLVITGREKDIIFVNGqnvyphDIER 408
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 496 SALEVERHLLAHphitdVAVIGPPDMVWGQ-RVSAVVQLRKgemlSVKDLKDWARDT------MAPYTIpTELIVVEEIP 568
Cdd:cd05908 409 IAEELEGVELGR-----VVACGVNNSNTRNeEIFCFIEHRK----SEDDFYPLGKKIkkhlnkRGGWQI-NEVLPIRRIP 478
|
570
....*....|....*.
gi 2452540125 569 RNQMGKVNKKELLQRF 584
Cdd:cd05908 479 KTTSGKVKRYELAQRY 494
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
64-580 |
1.40e-22 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 100.71 E-value: 1.40e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEIcraLDCSSRdlKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTEL 143
Cdd:cd17645 13 DHVAVVDRGQSLTYKQLNEKANQLARHL---RGKGVK--PDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYPGERI 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 144 EYVIQDSQSALVIAaeeyvgkitpSAEKLgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTGRPKG 223
Cdd:cd17645 88 AYMLADSSAKILLT----------NPDDL------------------------------------AYVIYTSGSTGLPKG 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 224 VLSTHENvqavttgLVEKWEWKK-------EDVILhVLPLHHVHGVINKLLCPLWVGATCVMLPefnaqmvwkkllssQA 296
Cdd:cd17645 122 VMIEHHN-------LVNLCEWHRpyfgvtpADKSL-VYASFSFDASAWEIFPHLTAGAALHVVP--------------SE 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 297 PRISVfmavptiyAKLVEYYDKH-----FFQPQVQDFVRAFCQENIRLMVSGSAALPVPVlEKwksitGHTLLERYGMTE 371
Cdd:cd17645 180 RRLDL--------DALNDYFNQEgitisFLPTGAAEQFMQLDNQSLRVLLTGGDKLKKIE-RK-----GYKLVNNYGPTE 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 372 IG-MALSNPLHGVRIPGSVGTPLPGVEVCIATETLkdgarsytihaqgdesntQVTPglEGQEGELLVRGPSVFREYWNR 450
Cdd:cd17645 246 NTvVATSFEIDKPYANIPIGKPIDNTRVYILDEAL------------------QLQP--IGVAGELCIAGEGLARGYLNR 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 451 PRETEDAFTPDGW------FKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMvw 523
Cdd:cd17645 306 PELTAEKFIVHPFvpgermYRTGDLAKFlPDGNIEFLGRLD-QQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDA-- 382
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 524 GQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17645 383 DGRKYLVAYVTAPEEIPHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
76-518 |
1.99e-22 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 101.49 E-value: 1.99e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSQeicrALdcSSRDLKEER-ISFLCPNDASYVV----AQWAswmsgGIAV----PLY--KKHPVTELE 144
Cdd:PRK08180 71 TYAEALERVRAIAQ----AL--LDRGLSAERpLMILSGNSIEHALlalaAMYA-----GVPYapvsPAYslVSQDFGKLR 139
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 145 YVIQDSQSALVIA--AEEYVGKITpSAEKLGVPVLpLLRSCSDGSAS-----------HPAVEE-----GPLTTcsswkn 206
Cdd:PRK08180 140 HVLELLTPGLVFAddGAAFARALA-AVVPADVEVV-AVRGAVPGRAAtpfaallatppTAAVDAahaavGPDTI------ 211
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 207 rgAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKED--VILHVLPLHHVHGVINKLLCPLWVGATCVM-----L 279
Cdd:PRK08180 212 --AKFLFTSGSTGLPKAVINTHRMLCANQQMLAQTFPFLAEEppVLVDWLPWNHTFGGNHNLGIVLYNGGTLYIddgkpT 289
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 280 PEFNAQMVwkKLLSSQAPriSVFMAVPTIYAKLVEYYDK------HFFqpqvqdfvrafcqENIRLMVSGSAALPVPVLE 353
Cdd:PRK08180 290 PGGFDETL--RNLREISP--TVYFNVPKGWEMLVPALERdaalrrRFF-------------SRLKLLFYAGAALSQDVWD 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 354 KWKSITGHTLLER------YGMTEIG-MALSNPLHGVRiPGSVGTPLPGVEVCIATetlkdgarsytihaqgdesntqvt 426
Cdd:PRK08180 353 RLDRVAEATCGERirmmtgLGMTETApSATFTTGPLSR-AGNIGLPAPGCEVKLVP------------------------ 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 427 pglEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAYKD------GVYWiKGRTSVDiiknggFKIS---- 496
Cdd:PRK08180 408 ---VGGKLEVRVKGPNVTPGYWRAPELTAEAFDEEGYYRSGDAVRFVDpadperGLMF-DGRIAED------FKLSsgtw 477
|
490 500
....*....|....*....|....*...
gi 2452540125 497 ----ALEVerHLLAH--PHITDVAVIGP 518
Cdd:PRK08180 478 vsvgPLRA--RAVSAgaPLVQDVVITGH 503
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
76-575 |
3.02e-22 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 101.03 E-value: 3.02e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSQEIcRALdcssRDLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALV 155
Cdd:cd05968 93 TYGELLYEVKRLANGL-RAL----GVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATRLQDAEAKAL 167
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 156 IAAEEYV--GKI---TPSAEK----------------LGVPVLPLL---RSCSDGSASHP--AVEEGPLTTCsswknrga 209
Cdd:cd05968 168 ITADGFTrrGREvnlKEEADKacaqcptvekvvvvrhLGNDFTPAKgrdLSYDEEKETAGdgAERTESEDPL-------- 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 210 MIIYTSGTTGRPKGVLSTHEN--VQAVTTgLVEKWEWKKEDVILHVLPLHHVHGVInKLLCPLWVGATCVM---LPEF-N 283
Cdd:cd05968 240 MIIYTSGTTGKPKGTVHVHAGfpLKAAQD-MYFQFDLKPGDLLTWFTDLGWMMGPW-LIFGGLILGATMVLydgAPDHpK 317
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 284 AQMVWKKLlssQAPRISVFMAVPTIYAKLVeyydkhffqPQVQDFVRAFCQENIRLMvsGSAALPVPvLEKWKSITGHTL 363
Cdd:cd05968 318 ADRLWRMV---EDHEITHLGLSPTLIRALK---------PRGDAPVNAHDLSSLRVL--GSTGEPWN-PEPWNWLFETVG 382
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 364 LER-----Y-GMTEI--GMALSNPLHGVRiPGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPglegQEGE 435
Cdd:cd05968 383 KGRnpiinYsGGTEIsgGILGNVLIKPIK-PSSFNGPVPGMKADVL-----------------DESGKPARP----EVGE 440
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 436 LLVRGP--SVFREYWNRPRETEDAF---TPDGWFKtGDTAAY-KDGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPH 509
Cdd:cd05968 441 LVLLAPwpGMTRGFWRDEDRYLETYwsrFDNVWVH-GDFAYYdEEGYFYILGR-SDDTINVAGKRVGPAEIESVLNAHPA 518
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2452540125 510 ITDVAVIGPPDMVWGQRVSAVVQLRKGEMLS---VKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKV 575
Cdd:cd05968 519 VLESAAIGVPHPVKGEAIVCFVVLKPGVTPTealAEELMERVADELGKPLSPERILFVKDLPKTRNAKV 587
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
64-580 |
9.96e-22 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 98.24 E-value: 9.96e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEHTYRDLLSQSLRLSQEICraldcSSRDLK-EERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTE 142
Cdd:cd17648 2 DRVAVVYGDKRLTYRELNERANRLAHYLL-----SVAEIRpDDLVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDER 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 143 LEYVIQDSQSALVIAaeeyvgkitpSAEKLgvpvlpllrscsdgsashpaveegplttcsswknrgAMIIYTSGTTGRPK 222
Cdd:cd17648 77 IQFILEDTGARVVIT----------NSTDL------------------------------------AYAIYTSGTTGKPK 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 223 GVLSTHENVQAVTTGLVEKWEWKKED--VIL----HVLPlHHVHgvinKLLCPLWVGATCVMLPE--------FNAQMvw 288
Cdd:cd17648 111 GVLVEHGSVVNLRTSLSERYFGRDNGdeAVLffsnYVFD-FFVE----QMTLALLNGQKLVVPPDemrfdpdrFYAYI-- 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 kkllssQAPRISVFMAVPTIyakLVEYydkhffqpqvqDFVRAfcqENIRLMVSGSAALPVPVLEKWKSITGHTLLERYG 368
Cdd:cd17648 184 ------NREKVTYLSGTPSV---LQQY-----------DLARL---PHLKRVDAAGEEFTAPVFEKLRSRFAGLIINAYG 240
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 369 MTEIGM-ALSNPLHGV-RIPGSVGTPLPGVevciatetlkdgaRSYTIHAqgdesNTQVTPglEGQEGELLVRGPSVFRE 446
Cdd:cd17648 241 PTETTVtNHKRFFPGDqRFDKSLGRPVRNT-------------KCYVLND-----AMKRVP--VGAVGELYLGGDGVARG 300
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 447 YWNRPRETEDAFTPDGW--------------FKTGDTAAYK-DGVYWIKGRTSVDIiKNGGFKISALEVERHLLAHPHIT 511
Cdd:cd17648 301 YLNRPELTAERFLPNPFqteqerargrnarlYKTGDLVRWLpSGELEYLGRNDFQV-KIRGQRIEPGEVEAALASYPGVR 379
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2452540125 512 DVAVIGP--PDMVWGQRVSAVV--QLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17648 380 ECAVVAKedASQAQSRIQKYLVgyYLPEPGHVPESDLLSFLRAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
67-580 |
3.04e-21 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 98.58 E-value: 3.04e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 67 AIIDQNGEHTYRDLLSQSLRLSQEIcraldcSSRDLKEERISFLC-PNDASYVVAQWASWMSGGIAVPLYKKHPVTELEY 145
Cdd:PRK10252 476 ALADARYQFSYREMREQVVALANLL------RERGVKPGDSVAVAlPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKM 549
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 146 VIQDSQSALVIAAEEYVGKITpsaeklGVPVLPLLrsCSDGSASHPAVEEGPLTTCSSwknrGAMIIYTSGTTGRPKGVL 225
Cdd:PRK10252 550 MLEDARPSLLITTADQLPRFA------DVPDLTSL--CYNAPLAPQGAAPLQLSQPHH----TAYIIFTSGSTGRPKGVM 617
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 226 STHenvqavtTGLVEKWEWKKE-------DVILHVLPLHHVHGVInKLLCPLWVGATCVML-PEFNAQMVW-KKLLSSQa 296
Cdd:PRK10252 618 VGQ-------TAIVNRLLWMQNhypltadDVVLQKTPCSFDVSVW-EFFWPFIAGAKLVMAePEAHRDPLAmQQFFAEY- 688
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 297 pRISVFMAVPTIYAKLVEYYDKHFFQPQVQDFVRAFCqenirlmvSGSaALPVPVLEKWKSITGHTLLERYGMTEIGMAL 376
Cdd:PRK10252 689 -GVTTTHFVPSMLAAFVASLTPEGARQSCASLRQVFC--------SGE-ALPADLCREWQQLTGAPLHNLYGPTEAAVDV 758
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 377 SN-PLHGVRIPGSVGTPLP-GVEVC-IATETLKDGARSytihaqgdesntqVTPGLegqEGELLVRGPSVFREYWNRPRE 453
Cdd:PRK10252 759 SWyPAFGEELAAVRGSSVPiGYPVWnTGLRILDARMRP-------------VPPGV---AGDLYLTGIQLAQGYLGRPDL 822
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 454 TEDAFTPDGW------FKTGDTAAYK-DGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHITDVAVI------GPPD 520
Cdd:PRK10252 823 TASRFIADPFapgermYRTGDVARWLdDGAVEYLGR-SDDQLKIRGQRIELGEIDRAMQALPDVEQAVTHacvinqAAAT 901
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 521 MVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PRK10252 902 GGDARQLVGYLVSQSGLPLDTSALQAQLRERLPPHMVPVVLLQLDQLPLSANGKLDRKAL 961
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
161-482 |
8.43e-21 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 96.58 E-value: 8.43e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 161 YVGKITPSAEKLGVPVLPLLRSCSDGsasHPAVEEGPLTTCSSwKNRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVE 240
Cdd:PTZ00216 223 YLDSLPASVDTEGCRLVAWTDVVAKG---HSAGSHHPLNIPEN-NDDLALIMYTSGTTGDPKGVMHTHGSLTAGILALED 298
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 241 KW-----EWKKEDVILHVLPLHHV--HGVINKLL---CPLWVGAT----------CVMLPEFNAQMV---------WKKL 291
Cdd:PTZ00216 299 RLndligPPEEDETYCSYLPLAHImeFGVTNIFLargALIGFGSPrtltdtfarpHGDLTEFRPVFLigvprifdtIKKA 378
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 292 LSSQAPRI-----SVF-MAVPTIYAKLVEYYDKHFFQPQVQDFVRAFCQENIRLMVSGSAALPVPVLEkWKSITGHTLLE 365
Cdd:PTZ00216 379 VEAKLPPVgslkrRVFdHAYQSRLRALKEGKDTPYWNEKVFSAPRAVLGGRVRAMLSGGGPLSAATQE-FVNVVFGMVIQ 457
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 366 RYGMTEI----GMALSNPLHgvriPGSVGTPLPGVEVCIA-TETLKdgarsytiHAQGDEsntqvtPglegqEGELLVRG 440
Cdd:PTZ00216 458 GWGLTETvccgGIQRTGDLE----PNAVGQLLKGVEMKLLdTEEYK--------HTDTPE------P-----RGEILLRG 514
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 2452540125 441 PSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGR 482
Cdd:PTZ00216 515 PFLFKGYYKQEELTREVLDEDGWFHTGDVGSIaANGTLRIIGR 557
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
209-536 |
9.90e-21 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 95.74 E-value: 9.90e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGvinkLLCplwvGATCVmLPEFN----A 284
Cdd:PRK09274 177 AAILFTSGSTGTPKGVVYTHGMFEAQIEALREDYGIEPGEIDLPTFPLFALFG----PAL----GMTSV-IPDMDptrpA 247
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 285 QMVWKKLLSS-QAPRISVFMAVPTIYAKLVEYYDKHffqpqvqdfVRAFcqENIRLMVSGSAALPVPVLEKWKSITGHT- 362
Cdd:PRK09274 248 TVDPAKLFAAiERYGVTNLFGSPALLERLGRYGEAN---------GIKL--PSLRRVISAGAPVPIAVIERFRAMLPPDa 316
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 363 -LLERYGMTE------IGMalSNPLHGVRI-----PGS-VGTPLPGVEVCIATetlkdgarsytIHAQGDESNTQVTPGL 429
Cdd:PRK09274 317 eILTPYGATEalpissIES--REILFATRAatdngAGIcVGRPVDGVEVRIIA-----------ISDAPIPEWDDALRLA 383
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 430 EGQEGELLVRGPSVFREYWNRPRETEDAFTPDG----WFKTGDtAAYKD--GVYWIKGRTSVDIIKNGG--FKIsalEVE 501
Cdd:PRK09274 384 TGEIGEIVVAGPMVTRSYYNRPEATRLAKIPDGqgdvWHRMGD-LGYLDaqGRLWFCGRKAHRVETAGGtlYTI---PCE 459
|
330 340 350
....*....|....*....|....*....|....*
gi 2452540125 502 RHLLAHPHITDVAVIGPPDMVwGQRVSAVVQLRKG 536
Cdd:PRK09274 460 RIFNTHPGVKRSALVGVGVPG-AQRPVLCVELEPG 493
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
209-576 |
1.06e-20 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 96.96 E-value: 1.06e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPefnaqmvw 288
Cdd:PRK06814 796 AVILFTSGSEGTPKGVVLSHRNLLANRAQVAARIDFSPEDKVFNALPVFHSFGLTGGLVLPLLSGVKVFLYP-------- 867
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 kkllSSQAPRIsvfmaVP--------TI----------YAKLVEYYDkhFFqpqvqdfvrafcqeNIRLMVSGSAALPVP 350
Cdd:PRK06814 868 ----SPLHYRI-----IPeliydtnaTIlfgtdtflngYARYAHPYD--FR--------------SLRYVFAGAEKVKEE 922
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 351 VLEKWKSITGHTLLERYGMTEIG--MALSNPLHGVriPGSVGTPLPGVEvciatetlkdgarsytihaqgdeSNTQVTPG 428
Cdd:PRK06814 923 TRQTWMEKFGIRILEGYGVTETApvIALNTPMHNK--AGTVGRLLPGIE-----------------------YRLEPVPG 977
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 429 LEgQEGELLVRGPSVFREYW--NRPRETEDAftPDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERhlL 505
Cdd:PRK06814 978 ID-EGGRLFVRGPNVMLGYLraENPGVLEPP--ADGWYDTGDIVTIdEEGFITIKGRAK-RFAKIAGEMISLAAVEE--L 1051
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 506 AhphitdvavigppDMVWGQRVSAVVQL---RKGEML---------SVKDLKDWARDTMAP-YTIPTELIVVEEIPRNQM 572
Cdd:PRK06814 1052 A-------------AELWPDALHAAVSIpdaRKGERIillttasdaTRAAFLAHAKAAGASeLMVPAEIITIDEIPLLGT 1118
|
....
gi 2452540125 573 GKVN 576
Cdd:PRK06814 1119 GKID 1122
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
209-474 |
1.45e-20 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 95.88 E-value: 1.45e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHE---NVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGViNKLLCP-LWVGATCVM-----L 279
Cdd:PRK12582 223 AKYLFTSGSTGMPKAVINTQRmmcANIAMQEQLRPREPDPPPPVSLDWMPWNHTMGG-NANFNGlLWGGGTLYIddgkpL 301
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 280 PEFNAQMVwkKLLSSQAPriSVFMAVPTIYAKLVEYYDKHffqpqvQDFVRAFCQeNIRLMVSGSAALPVPVLEKWK--- 356
Cdd:PRK12582 302 PGMFEETI--RNLREISP--TVYGNVPAGYAMLAEAMEKD------DALRRSFFK-NLRLMAYGGATLSDDLYERMQala 370
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 357 -SITGH--TLLERYGMTEIGMALSNPLHGVRIPGSVGTPLPGVEVciatetlkdgarsytihaqgdesntQVTPglEGQE 433
Cdd:PRK12582 371 vRTTGHriPFYTGYGATETAPTTTGTHWDTERVGLIGLPLPGVEL-------------------------KLAP--VGDK 423
|
250 260 270 280
....*....|....*....|....*....|....*....|.
gi 2452540125 434 GELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAYKD 474
Cdd:PRK12582 424 YEVRVKGPNVTPGYHKDPELTAAAFDEEGFYRLGDAARFVD 464
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
211-575 |
1.89e-20 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 95.46 E-value: 1.89e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 211 IIYTSGTTGRPKGVLS-----------THENVQAVTTGLVekWeWKKEDVILHVLPLHHVHGvinkllcPLWVGATCVML 279
Cdd:cd05967 235 ILYTSGTTGKPKGVVRdngghavalnwSMRNIYGIKPGDV--W-WAASDVGWVVGHSYIVYG-------PLLHGATTVLY 304
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 280 -------PEFNAqmvWKKLLSSQapRISVFMAVPTIYAKLveyydkHFFQPQvQDFVRAFCQENIRLMVSGSAALPVPVL 352
Cdd:cd05967 305 egkpvgtPDPGA---FWRVIEKY--QVNALFTAPTAIRAI------RKEDPD-GKYIKKYDLSSLRTLFLAGERLDPPTL 372
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 353 EKWKSITGHTLLERYGMTEIGMALSNPLHGVRI----PGSVGTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPG 428
Cdd:cd05967 373 EWAENTLGVPVIDHWWQTETGWPITANPVGLEPlpikAGSPGKPVPGYQVQVL-----------------DEDGEPVGPN 435
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 429 legQEGELLVRGP---SVFREYWNRPRETEDAF--TPDGWFKTGDtAAYKD--GVYWIKGRTSvDIIKNGGFKISALEVE 501
Cdd:cd05967 436 ---ELGNIVIKLPlppGCLLTLWKNDERFKKLYlsKFPGYYDTGD-AGYKDedGYLFIMGRTD-DVINVAGHRLSTGEME 510
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2452540125 502 RHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKD----WARDTMAPYTIPTELIVVEEIPRNQMGKV 575
Cdd:cd05967 511 ESVLSHPAVAECAVVGVRDELKGQVPLGLVVLKEGVKITAEELEKelvaLVREQIGPVAAFRLVIFVKRLPKTRSGKI 588
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
111-548 |
3.77e-20 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 94.52 E-value: 3.77e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 111 CPndaSYVVAQWASwMSGGIA-VPLYKKHPVTELEYVIQDSQSALVIAAEEYVGKITpSAEKLGVPVLPLLRSCSDGSAS 189
Cdd:PLN02861 112 CP---EWIIAMEAC-NSQGITyVPLYDTLGANAVEFIINHAEVSIAFVQESKISSIL-SCLPKCSSNLKTIVSFGDVSSE 186
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 190 HPAVEEGPLTTCSSWKNRGAM-----------------IIYTSGTTGRPKGVLSTHENVQA---VTTGLVEKWE--WKKE 247
Cdd:PLN02861 187 QKEEAEELGVSCFSWEEFSLMgsldcelppkqktdictIMYTSGTTGEPKGVILTNRAIIAevlSTDHLLKVTDrvATEE 266
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 248 DVILHVLPLHHVHGVINKLLCpLWVGATCvmlpEFNAQMVWKKLLSSQAPRISVFMAVPTIYAKLVE----------YYD 317
Cdd:PLN02861 267 DSYFSYLPLAHVYDQVIETYC-ISKGASI----GFWQGDIRYLMEDVQALKPTIFCGVPRVYDRIYTgimqkissggMLR 341
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 318 KHFFQ----PQVQDFVRAFCQEN--------------------IRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTE-I 372
Cdd:PLN02861 342 KKLFDfaynYKLGNLRKGLKQEEasprldrlvfdkikeglggrVRLLLSGAAPLPRHVEEFLRVTSCSVLSQGYGLTEsC 421
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 373 GMALSNPLHGVRIPGSVGTPLPGVEvciatetlkdgARSYTIHAQGDESNTQVTpglegqEGELLVRGPSVFREYWNRPR 452
Cdd:PLN02861 422 GGCFTSIANVFSMVGTVGVPMTTIE-----------ARLESVPEMGYDALSDVP------RGEICLRGNTLFSGYHKRQD 484
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 453 ETEDAFTpDGWFKTGDTAAYK-DGVYWIKGRtsvdiiKNGGFKIS-----ALEV-ERHLLAHPHITDVAVIGppDMVWGQ 525
Cdd:PLN02861 485 LTEEVLI-DGWFHTGDIGEWQpNGAMKIIDR------KKNIFKLSqgeyvAVENlENTYSRCPLIASIWVYG--NSFESF 555
|
490 500
....*....|....*....|...
gi 2452540125 526 RVSAVVQLRKGemlsvkdLKDWA 548
Cdd:PLN02861 556 LVAVVVPDRQA-------LEDWA 571
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
64-543 |
5.41e-20 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 93.65 E-value: 5.41e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 64 DKIAIIDQNGEH-----TYRDLLSQSLRLSQEICraldcsSRDLKEER-ISFLCPNDASYVVAQWASwMSGGIAV----P 133
Cdd:cd05921 10 DRTWLAEREGNGgwrrvTYAEALRQVRAIAQGLL------DLGLSAERpLLILSGNSIEHALMALAA-MYAGVPAapvsP 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 134 LY--KKHPVTELEYVIQDSQSALVIAAE-EYVGKITPSAEKLGVPVLPLLRSCS-DGSASHPAVEEGPLTTCSSwKNRGA 209
Cdd:cd05921 83 AYslMSQDLAKLKHLFELLKPGLVFAQDaAPFARALAAIFPLGTPLVVSRNAVAgRGAISFAELAATPPTAAVD-AAFAA 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 210 M-------IIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKED--VILHVLPLHHVHGVINKLLCPLWVGATCVM-- 278
Cdd:cd05921 162 VgpdtvakFLFTSGSTGLPKAVINTQRMLCANQAMLEQTYPFFGEEppVLVDWLPWNHTFGGNHNFNLVLYNGGTLYIdd 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 279 ---LPEFNAQMVwkKLLSSQAPriSVFMAVPTIYAKLVEYYDKhffqpqvQDFVRAFCQENIRLMVSGSAALPVPVLEKW 355
Cdd:cd05921 242 gkpMPGGFEETL--RNLREISP--TVYFNVPAGWEMLVAALEK-------DEALRRRFFKRLKLMFYAGAGLSQDVWDRL 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 356 KSI----TGH--TLLERYGMTEIGmALSNPLHGVR-IPGSVGTPLPGVEVCIATetlkdgarsytihaqgdesntqvtpg 428
Cdd:cd05921 311 QALavatVGEriPMMAGLGATETA-PTATFTHWPTeRSGLIGLPAPGTELKLVP-------------------------- 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 429 lEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAYKD------GVYWiKGRTSVDIIKNGGFKISA--LEV 500
Cdd:cd05921 364 -SGGKYEVRVKGPNVTPGYWRQPELTAQAFDEEGFYCLGDAAKLADpddpakGLVF-DGRVAEDFKLASGTWVSVgpLRA 441
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 2452540125 501 ERHLLAHPHITDVAVIGPPD-----MVWgQRVSAVVQLRKGEMLSVKD 543
Cdd:cd05921 442 RAVAACAPLVHDAVVAGEDRaevgaLVF-PDLLACRRLVGLQEASDAE 488
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
413-583 |
6.59e-19 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 90.05 E-value: 6.59e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 413 TIHAQG------DE---SNTQVTPGLEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGR 482
Cdd:PRK10946 351 IFTTQGrpmspdDEvwvADADGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLVSIdPDGYITVVGR 430
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 483 TSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRkgEMLSVKDLKDWARDT-MAPYTIPTEL 561
Cdd:PRK10946 431 EK-DQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVK--EPLKAVQLRRFLREQgIAEFKLPDRV 507
|
170 180
....*....|....*....|..
gi 2452540125 562 IVVEEIPRNQMGKVNKKELLQR 583
Cdd:PRK10946 508 ECVDSLPLTAVGKVDKKQLRQW 529
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
213-580 |
1.81e-18 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 88.64 E-value: 1.81e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 213 YTSGTTGRPKGVLSTHEN--VQAVTTGLVEKWEWKKEDVILHVLPLHHVHGvinklLCPLWV----GATCVMLPE-FNAQ 285
Cdd:cd05915 160 YTTGTTGLPKGVVYSHRAlvLHSLAASLVDGTALSEKDVVLPVVPMFHVNA-----WCLPYAatlvGAKQVLPGPrLDPA 234
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 286 MVWKKLLSSqapRISVFMAVPTIYAKLVeyydkhffqpQVQDFVRAFCQENIRLMVSGSAalPVPVLEKWKSITGHTLLE 365
Cdd:cd05915 235 SLVELFDGE---GVTFTAGVPTVWLALA----------DYLESTGHRLKTLRRLVVGGSA--APRSLIARFERMGVEVRQ 299
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 366 RYGMTEIgmalsnplHGVripGSVGTPLPGVEVCIATETLK----DGARSYTIHAQGDESNTQVTPGLEGQEGELLVRGP 441
Cdd:cd05915 300 GYGLTET--------SPV---VVQNFVKSHLESLSEEEKLTlkakTGLPIPLVRLRVADEEGRPVPKDGKALGEVQLKGP 368
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 442 SVFREYWNRPRETEDAFTPDGWFKTGDTAAYK-DGVYWIKGRtSVDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPD 520
Cdd:cd05915 369 WITGGYYGNEEATRSALTPDGFFRTGDIAVWDeEGYVEIKDR-LKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPH 447
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 521 MVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05915 448 PKWQERPLAVVVPRGEKPTPEELNEHLLKAGFAKWQLPDAYVFAEEIPRTSAGKFLKRAL 507
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
124-580 |
2.43e-18 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 89.84 E-value: 2.43e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 124 SWMSGGIAVPLYKKHPVTELEYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLPLLRSCSD----GSASH-PAVEEGPl 198
Cdd:PRK05691 3790 SFKAGAGYLPLDPGLPAQRLQRIIELSRTPVLVCSAACREQARALLDELGCANRPRLLVWEEvqagEVASHnPGIYSGP- 3868
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 199 ttcsswkNRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWvGATCVM 278
Cdd:PRK05691 3869 -------DNLAYVIYTSGSTGLPKGVMVEQRGMLNNQLSKVPYLALSEADVIAQTASQSFDISVWQFLAAPLF-GARVEI 3940
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 279 LPEFNAQMVWKKLLSSQAPRISVFMAVPTIYAKLVEYydkhffQPQVQDfvrafcqeNIRLMVSGSAALPVPVLEKWksi 358
Cdd:PRK05691 3941 VPNAIAHDPQGLLAHVQAQGITVLESVPSLIQGMLAE------DRQALD--------GLRWMLPTGEAMPPELARQW--- 4003
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 359 tghtlLERYgmTEIGmaLSNP-----------LHGVRIPGSVGTPLPgvevcIATETlkDGARSYTIhaqgDESNTQVTp 427
Cdd:PRK05691 4004 -----LQRY--PQIG--LVNAygpaecsddvaFFRVDLASTRGSYLP-----IGSPT--DNNRLYLL----DEALELVP- 4062
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 428 glEGQEGELLVRGPSVFREYWNRPRETEDAFTPDGW-------FKTGDTA-AYKDGVYWIKGRtsVD-IIKNGGFKISAL 498
Cdd:PRK05691 4063 --LGAVGELCVAGTGVGRGYVGDPLRTALAFVPHPFgapgerlYRTGDLArRRSDGVLEYVGR--IDhQVKIRGYRIELG 4138
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 499 EVERHLLAHPHITDVAVI---GPP-DMVWGQRVSAVVQLRKGEMLsvKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGK 574
Cdd:PRK05691 4139 EIEARLHEQAEVREAAVAvqeGVNgKHLVGYLVPHQTVLAQGALL--ERIKQRLRAELPDYMVPLHWLWLDRLPLNANGK 4216
|
....*.
gi 2452540125 575 VNKKEL 580
Cdd:PRK05691 4217 LDRKAL 4222
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
211-580 |
3.24e-18 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 88.42 E-value: 3.24e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 211 IIYTSGTTGRPKGVLSTHENVQAVT-TGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVML---PEF-NAQ 285
Cdd:PLN02654 280 LLYTSGSTGKPKGVLHTTGGYMVYTaTTFKYAFDYKPTDVYWCTADCGWITGHSYVTYGPMLNGATVLVFegaPNYpDSG 359
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 286 MVWKKLlssQAPRISVFMAVPTIYAKLVEYYDKhffqpqvqdFVRAFCQENIRLMvsGSAALPV-PVLEKW-KSITGHT- 362
Cdd:PLN02654 360 RCWDIV---DKYKVTIFYTAPTLVRSLMRDGDE---------YVTRHSRKSLRVL--GSVGEPInPSAWRWfFNVVGDSr 425
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 363 --LLERYGMTEIGMALSNPLHGV--RIPGSVGTPLPGVEVCIATEtlkdgarsytihaQGDEsntqvtpgLEGQ-EGELL 437
Cdd:PLN02654 426 cpISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDE-------------KGKE--------IEGEcSGYLC 484
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 438 VRG--PSVFRE-YWNRPRETEDAFTP-DGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITD 512
Cdd:PLN02654 485 VKKswPGAFRTlYGDHERYETTYFKPfAGYYFSGDGCSRdKDGYYWLTGRVD-DVINVSGHRIGTAEVESALVSHPQCAE 563
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2452540125 513 VAVIGPPDMVWGQRVSAVVQLRKGEMLSV---KDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:PLN02654 564 AAVVGIEHEVKGQGIYAFVTLVEGVPYSEelrKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 634
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
113-517 |
3.57e-18 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 88.18 E-value: 3.57e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 113 NDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSA-LVIAAEEYVGKITPSAEKL---------GVPV---LPL 179
Cdd:cd05933 42 NSPEWFIAAVGAIFAGGIAVGIYTTNSPEACQYVAETSEANiLVVENQKQLQKILQIQDKLphlkaiiqyKEPLkekEPN 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 180 LRSCS---DGSASHPavEEGPLTTCSSWK-NRGAMIIYTSGTTGRPKGVLSTHEN----VQAVTTGLVEKWEWKKEDVIL 251
Cdd:cd05933 122 LYSWDefmELGRSIP--DEQLDAIISSQKpNQCCTLIYTSGTTGMPKGVMLSHDNitwtAKAASQHMDLRPATVGQESVV 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 252 HVLPLHHVHGVINKLLCPLWVGAtCVMLPEFNAqMVWKKLLSSQAPRISVFMAVPTIYAKLVE----------------- 314
Cdd:cd05933 200 SYLPLSHIAAQILDIWLPIKVGG-QVYFAQPDA-LKGTLVKTLREVRPTAFMGVPRVWEKIQEkmkavgaksgtlkrkia 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 315 ---------------------YYDKHFFQPQVQDFVRAF-----CQENIrlmvSGSAALPVPVLEKWKSITgHTLLERYG 368
Cdd:cd05933 278 swakgvgletnlklmggespsPLFYRLAKKLVFKKVRKAlgldrCQKFF----TGAAPISRETLEFFLSLN-IPIMELYG 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 369 MTEigmalSNPLHGVRIPG-----SVGTPLPGVEVCIatetlkdgarsytihaqgdesntqVTPGLEGQeGELLVRGPSV 443
Cdd:cd05933 353 MSE-----TSGPHTISNPQayrllSCGKALPGCKTKI------------------------HNPDADGI-GEICFWGRHV 402
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 444 FREYWNRPRETEDAFTPDGWFKTGDTAAY-KDGVYWIKGRTSVDIIKNGGFKISALEVERHLLAH-PHITDVAVIG 517
Cdd:cd05933 403 FMGYLNMEDKTEEAIDEDGWLHSGDLGKLdEDGFLYITGRIKELIITAGGENVPPVPIEDAVKKElPIISNAMLIG 478
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
58-482 |
1.57e-16 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 84.06 E-value: 1.57e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 58 KALAFGDKIAI---IDQNGEH---TYRDLLSQSlrlsQEICRALdcSSRDLKEERISFLCPNDASYVVAQWASWMSGGIA 131
Cdd:PRK05691 18 RAAQTPDRLALrflADDPGEGvvlSYRDLDLRA----RTIAAAL--QARASFGDRAVLLFPSGPDYVAAFFGCLYAGVIA 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 132 VPLY-----KKHPVTELEYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLPLLrsCSDGSASHPAveegplttcSSWKN 206
Cdd:PRK05691 92 VPAYppesaRRHHQERLLSIIADAEPRLLLTVADLRDSLLQMEELAAANAPELL--CVDTLDPALA---------EAWQE 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 207 RG------AMIIYTSGTTGRPKGVLSTHENVQAVttglvekwEW----------KKEDVILHVLPLHHVHGVINKLLCPL 270
Cdd:PRK05691 161 PAlqpddiAFLQYTSGSTALPKGVQVSHGNLVAN--------EQlirhgfgidlNPDDVIVSWLPLYHDMGLIGGLLQPI 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 271 WVGATCVmlpefnaqmvwkkLLSSQaprisVFMAVPTIYAKLV-EYYDKHFFQPqvqDFVRAFCQENI------------ 337
Cdd:PRK05691 233 FSGVPCV-------------LMSPA-----YFLERPLRWLEAIsEYGGTISGGP---DFAYRLCSERVsesalerldlsr 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 338 -RLMVSGSAALPVPVL----EKWKS--ITGHTLLERYGMTEIGMALSNPLHGVRIP------------------GSV--- 389
Cdd:PRK05691 292 wRVAYSGSEPIRQDSLerfaEKFAAcgFDPDSFFASYGLAEATLFVSGGRRGQGIPaleldaealarnraepgtGSVlms 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 390 -GTPLPGVEVCIAtetlkdgarsytihaqgDESNTQVTPglEGQEGELLVRGPSVFREYWNRPRETEDAFTP-DG--WFK 465
Cdd:PRK05691 372 cGRSQPGHAVLIV-----------------DPQSLEVLG--DNRVGEIWASGPSIAHGYWRNPEASAKTFVEhDGrtWLR 432
|
490
....*....|....*..
gi 2452540125 466 TGDTAAYKDGVYWIKGR 482
Cdd:PRK05691 433 TGDLGFLRDGELFVTGR 449
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
209-555 |
5.50e-16 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 80.58 E-value: 5.50e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHhvhgvinKLLCPLwVGATCVmLPEFN----A 284
Cdd:cd05910 88 AAILFTSGSTGTPKGVVYRHGTFAAQIDALRQLYGIRPGEVDLATFPLF-------ALFGPA-LGLTSV-IPDMDptrpA 158
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 285 QMVWKKLLSS-QAPRISVFMAVPTIYAKLVEYYDKHFFQ-PQVqdfvrafcqeniRLMVSGSAALPVPVLEKWKSITGHT 362
Cdd:cd05910 159 RADPQKLVGAiRQYGVSIVFGSPALLERVARYCAQHGITlPSL------------RRVLSAGAPVPIALAARLRKMLSDE 226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 363 --LLERYGMTE---IGMALSNPLHGVRIPGS-------VGTPLPGVEVCIATETLKDgarsytIHAQGDesnTQVTPglE 430
Cdd:cd05910 227 aeILTPYGATEalpVSSIGSRELLATTTAATsggagtcVGRPIPGVRVRIIEIDDEP------IAEWDD---TLELP--R 295
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 431 GQEGELLVRGPSVFREYWNRPRETEDAFTPDG----WFKTGDTAAYKD-GVYWIKGRTSVDIIKNGGfKISALEVERHLL 505
Cdd:cd05910 296 GEIGEITVTGPTVTPTYVNRPVATALAKIDDNsegfWHRMGDLGYLDDeGRLWFCGRKAHRVITTGG-TLYTEPVERVFN 374
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|
gi 2452540125 506 AHPHITDVAVIGPPDMVwGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPY 555
Cdd:cd05910 375 THPGVRRSALVGVGKPG-CQLPVLCVEPLPGTITPRARLEQELRALAKDY 423
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
93-546 |
6.16e-16 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 80.82 E-value: 6.16e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 93 RALDCSSRDL-----KEERISFLCPNDASYVVAQWASWMSGGIAVPLY-------KKHPVTELEYVIQDSQSALVIAAEE 160
Cdd:PRK09192 58 RAEAGARRLLalglkPGDRVALIAETDGDFVEAFFACQYAGLVPVPLPlpmgfggRESYIAQLRGMLASAQPAAIITPDE 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 161 YVGKITPSAEKLgvpvlPLLRSCSDGSASHPAVEEGPLTTCSswKNRGAMIIYTSGTTGRPKGVLSTHE----NVQAVT- 235
Cdd:PRK09192 138 LLPWVNEATHGN-----PLLHVLSHAWFKALPEADVALPRPT--PDDIAYLQYSSGSTRFPRGVIITHRalmaNLRAISh 210
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 236 TGLvekwEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLP--EFNAQ-MVWKKLLSSQAPRISVfmaVPT----I 308
Cdd:PRK09192 211 DGL----KVRPGDRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLPtrDFARRpLQWLDLISRNRGTISY---SPPfgyeL 283
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 309 YAKLVEYYDKHFFQ----------------PQVQDFVRAFCQENIrlmvSGSAALPVpvlekwksitghtllerYGMTEI 372
Cdd:PRK09192 284 CARRVNSKDLAELDlscwrvagigadmirpDVLHQFAEAFAPAGF----DDKAFMPS-----------------YGLAEA 342
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 373 GMALSNPLHGvripgsvgtplPGVEV-CIATETLKDGARSYTIHAQGDESNT-----QVTPGLE-------GQE------ 433
Cdd:PRK09192 343 TLAVSFSPLG-----------SGIVVeEVDRDRLEYQGKAVAPGAETRRVRTfvncgKALPGHEieirneaGMPlpervv 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 434 GELLVRGPSVFREYWNRPrETEDAFTPDGWFKTGDTAAYKDGVYWIKGRTSVDIIKNGGfKISALEVERHLLAHP--HIT 511
Cdd:PRK09192 412 GHICVRGPSLMSGYFRDE-ESQDVLAADGWLDTGDLGYLLDGYLYITGRAKDLIIINGR-NIWPQDIEWIAEQEPelRSG 489
|
490 500 510
....*....|....*....|....*....|....*
gi 2452540125 512 DVAVIGPPDMVwGQRVSAVVQLRKGEMLSVKDLKD 546
Cdd:PRK09192 490 DAAAFSIAQEN-GEKIVLLVQCRISDEERRGQLIH 523
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
499-574 |
6.40e-16 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 72.58 E-value: 6.40e-16
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 499 EVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGK 574
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
76-499 |
7.17e-16 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 80.83 E-value: 7.17e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSLRLSQEIcraLDCSSRDlkEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALV 155
Cdd:PLN02614 81 TYQEVYDIVIKLGNSL---RSVGVKD--EAKCGIYGANSPEWIISMEACNAHGLYCVPLYDTLGAGAVEFIISHSEVSIV 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 156 IAAEEYVG---KITPSAEKLGVPVLpllrscSDGSASHPAVEEGPL--TTCSSW------------------KNRGAMII 212
Cdd:PLN02614 156 FVEEKKISelfKTCPNSTEYMKTVV------SFGGVSREQKEEAETfgLVIYAWdeflklgegkqydlpikkKSDICTIM 229
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 213 YTSGTTGRPKGVLSTHENVQAVTTGLVE-----KWEWKKEDVILHVLPLHHVHG-VINKllCPLWVGAtcvmlpefnAQM 286
Cdd:PLN02614 230 YTSGTTGDPKGVMISNESIVTLIAGVIRllksaNAALTVKDVYLSYLPLAHIFDrVIEE--CFIQHGA---------AIG 298
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 287 VWK---KLLSSQAPRI--SVFMAVPTIYAKLV-----EYYDKHFFQPQV---------------QDFVRA--FCQE---- 335
Cdd:PLN02614 299 FWRgdvKLLIEDLGELkpTIFCAVPRVLDRVYsglqkKLSDGGFLKKFVfdsafsykfgnmkkgQSHVEAspLCDKlvfn 378
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 336 --------NIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTE--IGMALSNPlHGVRIPGSVGTPLPGVEVCIAT--E 403
Cdd:PLN02614 379 kvkqglggNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTEscAGTFVSLP-DELDMLGTVGPPVPNVDIRLESvpE 457
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 404 TLKDGARSytihaqgdesntqvTPglegqEGELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTAAYK-DGVYWIKGR 482
Cdd:PLN02614 458 MEYDALAS--------------TP-----RGEICIRGKTLFSGYYKREDLTKEVLI-DGWLHTGDVGEWQpNGSMKIIDR 517
|
490
....*....|....*..
gi 2452540125 483 tsvdiiKNGGFKISALE 499
Cdd:PLN02614 518 ------KKNIFKLSQGE 528
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
194-550 |
5.59e-15 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 78.32 E-value: 5.59e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 194 EEGPLTTCSSWKNRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGL---VEKWEWK--KEDVILHVLPLHHV--------- 259
Cdd:PLN02430 208 KENPSETNPPKPLDICTIMYTSGTSGDPKGVVLTHEAVATFVRGVdlfMEQFEDKmtHDDVYLSFLPLAHIldrmieeyf 287
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 260 ----------HGVINKL------LCPLWVGATCVMLPEFNAQMvwKKLLSSQAP-RISVFMAvptIYAKLVEYYD---KH 319
Cdd:PLN02430 288 frkgasvgyyHGDLNALrddlmeLKPTLLAGVPRVFERIHEGI--QKALQELNPrRRLIFNA---LYKYKLAWMNrgySH 362
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 320 FFQPQVQDF-----VRAFCQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEI--GMALSNPlHGVRIPGSVGTP 392
Cdd:PLN02430 363 KKASPMADFlafrkVKAKLGGRLRLLISGGAPLSTEIEEFLRVTSCAFVVQGYGLTETlgPTTLGFP-DEMCMLGTVGAP 441
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 393 LPGVEVCIAtetlkdgarsytihaqgDESNTQVTPGLEGQEGELLVRGPSVFREYWNRPRETEDAFTpDGWFKTGDTAA- 471
Cdd:PLN02430 442 AVYNELRLE-----------------EVPEMGYDPLGEPPRGEICVRGKCLFSGYYKNPELTEEVMK-DGWFHTGDIGEi 503
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 472 YKDGVYWIKGRTSvDIIKNGGFKISALE-VERHLLAHPHITDVAVIGppDMVWGQRVSAVVqlrkgemLSVKDLKDWARD 550
Cdd:PLN02430 504 LPNGVLKIIDRKK-NLIKLSQGEYVALEyLENVYGQNPIVEDIWVYG--DSFKSMLVAVVV-------PNEENTNKWAKD 573
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
59-567 |
6.79e-15 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 77.61 E-value: 6.79e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 59 ALAFGDKIAIIDQNGEHTYRDLLSQSLRLSqeicRALdcSSRDLKE-ERISFLCPNDASYVVAqWASWMSGGIAVPLYKK 137
Cdd:PRK08279 47 AARHPDRPALLFEDQSISYAELNARANRYA----HWA--AARGVGKgDVVALLMENRPEYLAA-WLGLAKLGAVVALLNT 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 138 HPVTE-LEYVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLPL---------------LRSCSDGSASHPAVEEGPLTTc 201
Cdd:PRK08279 120 QQRGAvLAHSLNLVDAKHLIVGEELVEAFEEARADLARPPRLWvaggdtlddpegyedLAAAAAGAPTTNPASRSGVTA- 198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 202 sswkNRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPE 281
Cdd:PRK08279 199 ----KDTAFYIYTSGTTGLPKAAVMSHMRWLKAMGGFGGLLRLTPDDVLYCCLPLYHNTGGTVAWSSVLAAGATLALRRK 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 282 FNAQMVWKKLlssQAPRISVFMAVPTIYAKLVEYYDKHffqpqvQDfvRafcQENIRLMVsgSAALPVPVLEKWKSITG- 360
Cdd:PRK08279 275 FSASRFWDDV---RRYRATAFQYIGELCRYLLNQPPKP------TD--R---DHRLRLMI--GNGLRPDIWDEFQQRFGi 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 361 HTLLERYGMTEIGMALSNPLHgvrIPGSVG-TPLPG------VEVCIATETlkdgarsytiHAQGDESNTQVTPglEGQE 433
Cdd:PRK08279 339 PRILEFYAASEGNVGFINVFN---FDGTVGrVPLWLahpyaiVKYDVDTGE----------PVRDADGRCIKVK--PGEV 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 434 GELLVR-GPSVFREYWNRPRETE-----DAFTP-DGWFKTGDTAAY-KDGVYWIkgrtsVDII------KngGFKISALE 499
Cdd:PRK08279 404 GLLIGRiTDRGPFDGYTDPEASEkkilrDVFKKgDAWFNTGDLMRDdGFGHAQF-----VDRLgdtfrwK--GENVATTE 476
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2452540125 500 VERHLLAHPHITDVAVIGppdmvwgqrVS----------AVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEI 567
Cdd:PRK08279 477 VENALSGFPGVEEAVVYG---------VEvpgtdgragmAAIVLADGAEFDLAALAAHLYERLPAYAVPLFVRLVPEL 545
|
|
| PRK07769 |
PRK07769 |
long-chain-fatty-acid--CoA ligase; Validated |
105-491 |
7.39e-15 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 181109 [Multi-domain] Cd Length: 631 Bit Score: 77.85 E-value: 7.39e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 105 ERISFLCPNDASYVVAQWASWMSGGIAVPLYK-KHP--VTELEYVIQDSQSALVIAAeeyvgkiTPSAEKlgvpVLPLLR 181
Cdd:PRK07769 80 DRVAILAPQNLDYLIAFFGALYAGRIAVPLFDpAEPghVGRLHAVLDDCTPSAILTT-------TDSAEG----VRKFFR 148
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 182 SCSdgSASHP---AVEEGPLTTCSSW------KNRGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILH 252
Cdd:PRK07769 149 ARP--AKERPrviAVDAVPDEVGATWvppeanEDTIAYLQYTSGSTRIPAGVQITHLNLPTNVLQVIDALEGQEGDRGVS 226
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 253 VLPLHHVHGVINKLLCPLWVGATCVMLPefnAQMV-----WKKLLSSQAP-RISVFMAVPTIYAKLVEyydkhffqpqvq 326
Cdd:PRK07769 227 WLPFFHDMGLITVLLPALLGHYITFMSP---AAFVrrpgrWIRELARKPGgTGGTFSAAPNFAFEHAA------------ 291
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 327 dfVRAFCQE--------NIRLMVSGSAALPVPVLEKWKSITGHTLLER------YGMTEIGMALSNPLHGVRipgsvgtp 392
Cdd:PRK07769 292 --ARGLPKDgeppldlsNVKGLLNGSEPVSPASMRKFNEAFAPYGLPPtaikpsYGMAEATLFVSTTPMDEE-------- 361
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 393 lPGVeVCIATETLKDGaRSYTIHAQGDESNTQVTPGL-------------------EGQEGELLVRGPSVFREYWNRPRE 453
Cdd:PRK07769 362 -PTV-IYVDRDELNAG-RFVEVPADAPNAVAQVSAGKvgvsewavivdpetaselpDGQIGEIWLHGNNIGTGYWGKPEE 438
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*
gi 2452540125 454 TEDAF---------------TPDG--WFKTGDTAAYKDGVYWIKGRTSVDIIKNG 491
Cdd:PRK07769 439 TAATFqnilksrlseshaegAPDDalWVRTGDYGVYFDGELYITGRVKDLVIIDG 493
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
152-558 |
2.47e-14 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 75.79 E-value: 2.47e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 152 SALVIAAE--EYVGKITPSAEKLGVPVLPLLRSCS-----------DGSASHPAVEEgpLTTCSSWKNRgAMIIYTSGTT 218
Cdd:cd05938 80 KVLVVAPElqEAVEEVLPALRADGVSVWYLSHTSNtegvislldkvDAASDEPVPAS--LRAHVTIKSP-ALYIYTSGTT 156
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 219 GRPKGVLSTHENVQAVTtGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVWKkllSSQAPR 298
Cdd:cd05938 157 GLPKAARISHLRVLQCS-GFLSLCGVTADDVIYITLPLYHSSGFLLGIGGCIELGATCVLKPKFSASQFWD---DCRKHN 232
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 299 ISVFMAVPTIYAKLVEyydkhffQPQ-VQDfvrafCQENIRLMVsGSAALPvpvlEKWKSIT---GHT-LLERYGMTEIG 373
Cdd:cd05938 233 VTVIQYIGELLRYLCN-------QPQsPND-----RDHKVRLAI-GNGLRA----DVWREFLrrfGPIrIREFYGSTEGN 295
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 374 MALSNPlhgVRIPGSVG---------TPLPGVEVCIATETLKDGARSYTIHAQGDESNTQVTPglegqegellVRGPSVF 444
Cdd:cd05938 296 IGFFNY---TGKIGAVGrvsylykllFPFELIKFDVEKEEPVRDAQGFCIPVAKGEPGLLVAK----------ITQQSPF 362
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 445 REYWNRPRETE-----DAFTP-DGWFKTGDT-AAYKDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIG 517
Cdd:cd05938 363 LGYAGDKEQTEkkllrDVFKKgDVYFNTGDLlVQDQQNFLYFHDRVG-DTFRWKGENVATTEVADVLGLLDFLQEVNVYG 441
|
410 420 430 440
....*....|....*....|....*....|....*....|....
gi 2452540125 518 PPdmVWGQ--RVS-AVVQLRKGEMLSVKDLKDWARDTMAPYTIP 558
Cdd:cd05938 442 VT--VPGHegRIGmAAVKLKPGHEFDGKKLYQHVREYLPAYARP 483
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
209-580 |
9.22e-14 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 73.62 E-value: 9.22e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGV-LSTHENVqaVTTGLVEKW-EWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQM 286
Cdd:cd05937 90 AILIYTSGTTGLPKAAaISWRRTL--VTSNLLSHDlNLKNGDRTYTCMPLYHGTAAFLGACNCLMSGGTLALSRKFSASQ 167
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 287 VWKKLLSSQAPRI-------SVFMAVPtiyaklVEYYDKhffqpqvqdfvrafcQENIRlMVSGSAALPvPVLEKWKsit 359
Cdd:cd05937 168 FWKDVRDSGATIIqyvgelcRYLLSTP------PSPYDR---------------DHKVR-VAWGNGLRP-DIWERFR--- 221
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 360 ghtllERYGMTEIG---------MALSNPLHGVRIPGSVGT-------PLPGVEVCIA--TET---LKDGARSYTIHAQg 418
Cdd:cd05937 222 -----ERFNVPEIGefyaategvFALTNHNVGDFGAGAIGHhglirrwKFENQVVLVKmdPETddpIRDPKTGFCVRAP- 295
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 419 desntqvtpglEGQEGELLVRGP----SVFREYWNRPRETE-----DAFTP-DGWFKTGDTA-AYKDGVYWIKGRTSvDI 487
Cdd:cd05937 296 -----------VGEPGEMLGRVPfknrEAFQGYLHNEDATEsklvrDVFRKgDIYFRTGDLLrQDADGRWYFLDRLG-DT 363
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 488 IKNGGFKISALEVERHLLAHPHITDVAVIG---PPDMvwGQRVSAVVQLRKGEmlSVKDLKDW------ARDTMAPYTIP 558
Cdd:cd05937 364 FRWKSENVSTTEVADVLGAHPDIAEANVYGvkvPGHD--GRAGCAAITLEESS--AVPTEFTKsllaslARKNLPSYAVP 439
|
410 420
....*....|....*....|..
gi 2452540125 559 TELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd05937 440 LFLRLTEEVATTDNHKQQKGVL 461
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
207-561 |
1.95e-13 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 72.60 E-value: 1.95e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 207 RGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGV-InkllcpLW----VGATCVmLPE 281
Cdd:PRK09029 136 RLATMTLTSGSTGLPKAAVHTAQAHLASAEGVLSLMPFTAQDSWLLSLPLFHVSGQgI------VWrwlyAGATLV-VRD 208
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 282 FN--AQMVwkkllsSQAPRISVfmaVPTIYAKLVEYydkhffqPQVQDFVRAFcqenirLMvsGSAALPvpvlekwksit 359
Cdd:PRK09029 209 KQplEQAL------AGCTHASL---VPTQLWRLLDN-------RSEPLSLKAV------LL--GGAAIP----------- 253
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 360 gHTLLER-----------YGMTEigMAlsNPLHGVRIPGS--VGTPLPGVEVCIAtetlkdgarsytihaqgdesntqvt 426
Cdd:PRK09029 254 -VELTEQaeqqgircwcgYGLTE--MA--STVCAKRADGLagVGSPLPGREVKLV------------------------- 303
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 427 pglegqEGELLVRGPSVFREYWnRPRETEDAFTPDGWFKTGDTAAYKDGVYWIKGRtsvdiIKN----GGFKISALEVER 502
Cdd:PRK09029 304 ------DGEIWLRGASLALGYW-RQGQLVPLVNDEGWFATRDRGEWQNGELTILGR-----LDNlffsGGEGIQPEEIER 371
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2452540125 503 HLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEmlSVKDLKDWARDTMA----P---YTIPTEL 561
Cdd:PRK09029 372 VINQHPLVQQVFVVPVADAEFGQRPVAVVESDSEA--AVVNLAEWLQDKLArfqqPvayYLLPPEL 435
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
76-566 |
5.48e-13 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 71.72 E-value: 5.48e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 76 TYRDLLSQSlrlsQEICRALDCSSRDLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQDSQSALV 155
Cdd:cd17632 69 TYAELWERV----GAVAAAHDPEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEPRLL 144
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 156 IAAEEYVGKIT------PSAEKLGV----PVLPLLRSCSDGSASHPAVEEGPLTTCSSWKNRG----------------- 208
Cdd:cd17632 145 AVSAEHLDLAVeavlegGTPPRLVVfdhrPEVDAHRAALESARERLAAVGIPVTTLTLIAVRGrdlppaplfrpepdddp 224
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 -AMIIYTSGTTGRPKGVLSTHENVqaVTTGLVEKWE---WKKEDVILHVLPLHHVHGViNKLLCPLWVGATCVMLPEFNA 284
Cdd:cd17632 225 lALLIYTSGSTGTPKGAMYTERLV--ATFWLKVSSIqdiRPPASITLNFMPMSHIAGR-ISLYGTLARGGTAYFAAASDM 301
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 285 QMVWKKLlssQAPRISVFMAVPTIYAKLVEYY----DKHFFQPQVQDF----VRAFCQENI---RLM--VSGSAALPVPV 351
Cdd:cd17632 302 STLFDDL---ALVRPTELFLVPRVCDMLFQRYqaelDRRSVAGADAETlaerVKAELRERVlggRLLaaVCGSAPLSAEM 378
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 352 LEKWKSITGHTLLERYGMTEIGMALsnpLHGV--RIP------------GSVGTPLPgvevciatetlkdgarsytiHAQ 417
Cdd:cd17632 379 KAFMESLLDLDLHDGYGSTEAGAVI---LDGVivRPPvldyklvdvpelGYFRTDRP--------------------HPR 435
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 418 gdesntqvtpglegqeGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGDTAAY--KDGVYWIKGRTSVDIIKNGGF-K 494
Cdd:cd17632 436 ----------------GELLVKTDTLFPGYYKRPEVTAEVFDEDGFYRTGDVMAElgPDRLVYVDRRNNVLKLSQGEFvT 499
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 495 ISALEV-----------------ERHLLahphitdVAVIGPPDMVWGQRVSAVVQLRKGEmlsvkDLKDWARDT-MAPYT 556
Cdd:cd17632 500 VARLEAvfaasplvrqifvygnsERAYL-------LAVVVPTQDALAGEDTARLRAALAE-----SLQRIAREAgLQSYE 567
|
570
....*....|
gi 2452540125 557 IPTELIVVEE 566
Cdd:cd17632 568 IPRDFLIETE 577
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
70-519 |
7.76e-13 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 71.00 E-value: 7.76e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 70 DQNGEHTYRDLLSQSLRLSQEICRaldcssrdLKEERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEYVIQD 149
Cdd:PRK06334 41 EQLGKLSYNQVRKAVIALATKVSK--------YPDQHIGIMMPASAGAYIAYFATLLSGKIPVMINWSQGLREVTACANL 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 150 SQSALVIAAEEYVGKITPS-AEKLGVPV-----------LPLLRSCSDG---SASHPAV-------EEGPLTTcsswknr 207
Cdd:PRK06334 113 VGVTHVLTSKQLMQHLAQThGEDAEYPFsliymeevrkeLSFWEKCRIGiymSIPFEWLmrwfgvsDKDPEDV------- 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 208 gAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGaTCVmlpefnaqmv 287
Cdd:PRK06334 186 -AVILFTSGTEKLPKGVPLTHANLLANQRACLKFFSPKEDDVMMSFLPPFHAYGFNSCTLFPLLSG-VPV---------- 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 288 wkkllssqaprisVFMAVPTIYAKLVEYYDKH---FF--QPQVQDFV------RAFCQENIRLMVSGSAALPVPVLEKWK 356
Cdd:PRK06334 254 -------------VFAYNPLYPKKIVEMIDEAkvtFLgsTPVFFDYIlktakkQESCLPSLRFVVIGGDAFKDSLYQEAL 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 357 SITGH-TLLERYGMTEIGMALS-NPLHGVRIPGSVGTPLPGVEVCIATETLKdgarsytihaqgdesntqvTPGLEGQEG 434
Cdd:PRK06334 321 KTFPHiQLRQGYGTTECSPVITiNTVNSPKHESCVGMPIRGMDVLIVSEETK-------------------VPVSSGETG 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 435 ELLVRGPSVFREYW-NRPRETEDAFTPDGWFKTGDTaAYKD--GVYWIKGRTSvDIIKNGGFKISALEVERHLLAH---- 507
Cdd:PRK06334 382 LVLTRGTSLFSGYLgEDFGQGFVELGGETWYVTGDL-GYVDrhGELFLKGRLS-RFVKIGAEMVSLEALESILMEGfgqn 459
|
490
....*....|....
gi 2452540125 508 --PHITDVAVIGPP 519
Cdd:PRK06334 460 aaDHAGPLVVCGLP 473
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
209-482 |
1.18e-12 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 70.89 E-value: 1.18e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHE----NVQAVTTGLvekwEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNA 284
Cdd:PRK08043 368 ALILFTSGSEGHPKGVVHSHKsllaNVEQIKTIA----DFTPNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLH 443
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 285 QMVWKKLLSSQapRISVFMAVPTI---YAKLVEYYdkhffqpqvqDFVRafcqenIRLMVSGSAALPVPVLEKWKSITGH 361
Cdd:PRK08043 444 YRIVPELVYDR--NCTVLFGTSTFlgnYARFANPY----------DFAR------LRYVVAGAEKLQESTKQLWQDKFGL 505
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 362 TLLERYGMTEIG--MALSNPLHGVriPGSVGTPLPGVEvciatetlkdgARSYTIhaqgdesntqvtPGLEgQEGELLVR 439
Cdd:PRK08043 506 RILEGYGVTECApvVSINVPMAAK--PGTVGRILPGMD-----------ARLLSV------------PGIE-QGGRLQLK 559
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|...
gi 2452540125 440 GPSVFREYW--NRPRETEDAFTPD-------GWFKTGDTAAYKD-GVYWIKGR 482
Cdd:PRK08043 560 GPNIMNGYLrvEKPGVLEVPTAENargemerGWYDTGDIVRFDEqGFVQIQGR 612
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
119-502 |
2.79e-12 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 69.25 E-value: 2.79e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 119 VAQwASWMSGGIAVPLYKKHPVTELEYVIQDS-------QSALVIAAEEYVGKItPSAEKLGVPVLPLLRSCSDGSASHP 191
Cdd:PRK07768 70 TAQ-GLWMRGASLTMLHQPTPRTDLAVWAEDTlrvigmiGAKAVVVGEPFLAAA-PVLEEKGIRVLTVADLLAADPIDPV 147
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 192 AVEEGPLttcsswknrgAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKE-DVILHVLPLHHVHGVINKLLCPL 270
Cdd:PRK07768 148 ETGEDDL----------ALMQLTSGSTGSPKAVQITHGNLYANAEAMFVAAEFDVEtDVMVSWLPLFHDMGMVGFLTVPM 217
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 271 WVGATCVML-P-EFNAQ-MVWKKLLSSQAPRISvfmAVP----TIYAKLVEyydkhffqpqVQDFVRAFCQENIRLMVSG 343
Cdd:PRK07768 218 YFGAELVKVtPmDFLRDpLLWAELISKYRGTMT---AAPnfayALLARRLR----------RQAKPGAFDLSSLRFALNG 284
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 344 SAALPVPVLEKWKSITG------HTLLERYGMTEIGMALSNP------------------LH--------GVRIPGSVGT 391
Cdd:PRK07768 285 AEPIDPADVEDLLDAGArfglrpEAILPAYGMAEATLAVSFSpcgaglvvdevdadllaaLRravpatkgNTRRLATLGP 364
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 392 PLPGVEVCIATEtlkDGarsytihaqgdesntQVTPglEGQEGELLVRGPSVFREYwnrprETEDAFTP----DGWFKTG 467
Cdd:PRK07768 365 PLPGLEVRVVDE---DG---------------QVLP--PRGVGVIELRGESVTPGY-----LTMDGFIPaqdaDGWLDTG 419
|
410 420 430
....*....|....*....|....*....|....*..
gi 2452540125 468 DTaAY--KDGVYWIKGRTSvDIIKNGGFKISALEVER 502
Cdd:PRK07768 420 DL-GYltEEGEVVVCGRVK-DVIIMAGRNIYPTDIER 454
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
209-580 |
6.83e-12 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 67.88 E-value: 6.83e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCpLWVGATCVMLPEFNAQMVw 288
Cdd:cd17654 121 AYVIHTSGTTGTPKIVAVPHKCILPNIQHFRSLFNITSEDILFLTSPLTFDPSVVEIFLS-LSSGATLLIVPTSVKVLP- 198
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 KKLLSS--QAPRISVFMAVPTIYaklveyydKHFFQPQVQDFVRAfCQENIRLMVSGSAALPVPVLEK-WKSITGHT-LL 364
Cdd:cd17654 199 SKLADIlfKRHRITVLQATPTLF--------RRFGSQSIKSTVLS-ATSSLRVLALGGEPFPSLVILSsWRGKGNRTrIF 269
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 365 ERYGMTEIGM-ALSNPLHGVRIPGSVGTPLPGVEVciatetlkdgarsytihaqgdESNTQVTPGLEGQ-EGELLVRGpS 442
Cdd:cd17654 270 NIYGITEVSCwALAYKVPEEDSPVQLGSPLLGTVI---------------------EVRDQNGSEGTGQvFLGGLNRV-C 327
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 443 VFREYWNRPRETedaftpdgWFKTGDTAAYKDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVigppdMV 522
Cdd:cd17654 328 ILDDEVTVPKGT--------MRATGDFVTVKDGELFFLGRKD-SQIKRRGKRINLDLIQQVIESCLGVESCAV-----TL 393
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*...
gi 2452540125 523 WGQRvsAVVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVNKKEL 580
Cdd:cd17654 394 SDQQ--RLIAFIVGESSSSRIHKELQLTLLSSHAIPDTFVQIDKLPLTSHGKVDKSEL 449
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
105-491 |
9.74e-12 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 67.84 E-value: 9.74e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 105 ERISFLCPNDASYVVAQWASWMSGGIAVPLYKK----HpvTE-LEYVIQDSQSALVI---AAEEYVGKITPSAEKLGVPV 176
Cdd:PRK12476 93 DRVAILAPQGIDYVAGFFAAIKAGTIAVPLFAPelpgH--AErLDTALRDAEPTVVLtttAAAEAVEGFLRNLPRLRRPR 170
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 177 LPLLRSCSDGSAShpAVEEGPLTTcsswkNRGAMIIYTSGTTGRPKGVLSTHenvQAVTTGLVEkwewkkedVILHV--- 253
Cdd:PRK12476 171 VIAIDAIPDSAGE--SFVPVELDT-----DDVSHLQYTSGSTRPPVGVEITH---RAVGTNLVQ--------MILSIdll 232
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 254 ---------LPLHHVHGVINKLLCPLWVGATCVMLPefnAQMV-----WKKLLSSQAPRISVFMAVPTiYAklVEYYDKH 319
Cdd:PRK12476 233 drnthgvswLPLYHDMGLSMIGFPAVYGGHSTLMSP---TAFVrrpqrWIKALSEGSRTGRVVTAAPN-FA--YEWAAQR 306
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 320 FFQPQvqdfvrafcQENIRL----MVSGSAALPVPVLEKWKSITGHTLLER------YGMTEIGMALSNPLHGVRiPGSV 389
Cdd:PRK12476 307 GLPAE---------GDDIDLsnvvLIIGSEPVSIDAVTTFNKAFAPYGLPRtafkpsYGIAEATLFVATIAPDAE-PSVV 376
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 390 GTPLPGVEVCIATETLKDGARSYTIHAQGDESNTQ----VTPGLE-----GQEGELLVRGPSVFREYWNRPRETEDAF-- 458
Cdd:PRK12476 377 YLDREQLGAGRAVRVAADAPNAVAHVSCGQVARSQwaviVDPDTGaelpdGEVGEIWLHGDNIGRGYWGRPEETERTFga 456
|
410 420 430 440
....*....|....*....|....*....|....*....|....*....
gi 2452540125 459 --------------TPDG--WFKTGDTAAYKDGVYWIKGRTSVDIIKNG 491
Cdd:PRK12476 457 klqsrlaegshadgAADDgtWLRTGDLGVYLDGELYITGRIADLIVIDG 505
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
209-517 |
3.99e-10 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 62.37 E-value: 3.99e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVGATCVMLPEFNAQMVW 288
Cdd:cd05940 84 ALYIYTSGTTGLPKAAIISHRRAWRGGAFFAGSGGALPSDVLYTCLPLYHSTALIVGWSACLASGATLVIRKKFSASNFW 163
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 KKLLSSQApriSVFMAVptiyAKLVEYYDKhffQPQVQDfVRAfcqENIRlMVSGSAALPvpvlEKWKSITGH----TLL 364
Cdd:cd05940 164 DDIRKYQA---TIFQYI----GELCRYLLN---QPPKPT-ERK---HKVR-MIFGNGLRP----DIWEEFKERfgvpRIA 224
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 365 ERYGMTEIGMALSNpLHGVriPGSVG---------TPLPGVEVCIATETLKDGARSYTIhaqgdesntQVTPgleGQEGE 435
Cdd:cd05940 225 EFYAATEGNSGFIN-FFGK--PGAIGrnpsllrkvAPLALVKYDLESGEPIRDAEGRCI---------KVPR---GEPGL 289
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 436 LLVR--GPSVFREYWNrPRETE-----DAFTP-DGWFKTGDTAAYKDGVYW-IKGRTSvDIIKNGGFKISALEVERHLLA 506
Cdd:cd05940 290 LISRinPLEPFDGYTD-PAATEkkilrDVFKKgDAWFNTGDLMRLDGEGFWyFVDRLG-DTFRWKGENVSTTEVAAVLGA 367
|
330
....*....|.
gi 2452540125 507 HPHITDVAVIG 517
Cdd:cd05940 368 FPGVEEANVYG 378
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
105-482 |
2.45e-09 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 59.96 E-value: 2.45e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 105 ERISFLCPNDASYVVAQWASWMSGGIAVPLYKKHPVTELEY---VIQDSQSALVI---AAEEYVGKITPSAEKLGVPVLP 178
Cdd:PRK05850 60 DRAVILAPQGLEYIVAFLGALQAGLIAVPLSVPQGGAHDERvsaVLRDTSPSVVLttsAVVDDVTEYVAPQPGQSAPPVI 139
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 179 LLRSCSDGSASHPAVEEGPLTTcsswknrGAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDV------ILH 252
Cdd:PRK05850 140 EVDLLDLDSPRGSDARPRDLPS-------TAYLQYTSGSTRTPAGVMVSHRNVIANFEQLMSDYFGDTGGVpppdttVVS 212
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 253 VLPLHHVHGVINKLLCPLWVGATCV-MLPEFNAQ--MVWKKLLSSQAPrisVFMAVPTIYAKLVeyydkhffqpqvqdfV 329
Cdd:PRK05850 213 WLPFYHDMGLVLGVCAPILGGCPAVlTSPVAFLQrpARWMQLLASNPH---AFSAAPNFAFELA---------------V 274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 330 RAFCQE--------NIRLMVSGSaalpvpvlEKWKSITGHTLLER--------------YGMTEIGMALSnplhgVRIPG 387
Cdd:PRK05850 275 RKTSDDdmagldlgGVLGIISGS--------ERVHPATLKRFADRfapfnlretairpsYGLAEATVYVA-----TREPG 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 388 SvgtplPGVEVCIATETLKDG------------------ARSYTIHAQGDESNTQVTPgleGQEGELLVRGPSVFREYWN 449
Cdd:PRK05850 342 Q-----PPESVRFDYEKLSAGhakrcetgggtplvsygsPRSPTVRIVDPDTCIECPA---GTVGEIWVHGDNVAAGYWQ 413
|
410 420 430 440
....*....|....*....|....*....|....*....|....
gi 2452540125 450 RPRETEDAF----------TPDG-WFKTGDTAAYKDGVYWIKGR 482
Cdd:PRK05850 414 KPEETERTFgatlvdpspgTPEGpWLRTGDLGFISEGELFIVGR 457
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
209-584 |
6.33e-09 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 58.97 E-value: 6.33e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 209 AMIIYTSGTTGRPKGVLSTHENVQAVTTG---LVEKWEwkKEDVILHVLPLHHVhgvinkllcpLWVGATCVMLPEFNAQ 285
Cdd:PLN02387 253 AVIMYTSGSTGLPKGVMMTHGNIVATVAGvmtVVPKLG--KNDVYLAYLPLAHI----------LELAAESVMAAVGAAI 320
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 286 MVWKKL-LSSQAPRI-------------SVFMAVPTI--------------------------YAKLVEYYDKHFF---- 321
Cdd:PLN02387 321 GYGSPLtLTDTSNKIkkgtkgdasalkpTLMTAVPAIldrvrdgvrkkvdakgglakklfdiaYKRRLAAIEGSWFgawg 400
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 322 -QPQVQDF-----VRAFCQENIRLMVSGSAALPvPVLEKWKSIT-GHTLLERYGMTEI--GMALSNPLH-GVripGSVGT 391
Cdd:PLN02387 401 lEKLLWDAlvfkkIRAVLGGRIRFMLSGGAPLS-GDTQRFINIClGAPIGQGYGLTETcaGATFSEWDDtSV---GRVGP 476
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 392 PLPgvevCiatetlkdgarSYTIHAQGDESNTQVT----PglegqEGELLVRGPSVFREYWNRPRETEDAFTPDG----W 463
Cdd:PLN02387 477 PLP----C-----------CYVKLVSWEEGGYLISdkpmP-----RGEIVIGGPSVTLGYFKNQEKTDEVYKVDErgmrW 536
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 464 FKTGDTAAYK-DGVYWIKGRTSvDIIK-NGGFKISALEVERHLLAHPHITDVAVIGPPdmVWGQRVSAVVQlrkgemlSV 541
Cdd:PLN02387 537 FYTGDIGQFHpDGCLEIIDRKK-DIVKlQHGEYVSLGKVEAALSVSPYVDNIMVHADP--FHSYCVALVVP-------SQ 606
|
410 420 430 440
....*....|....*....|....*....|....*....|....*..
gi 2452540125 542 KDLKDWARDTMAPYTIPTELI----VVEEIpRNQMGKVNKKELLQRF 584
Cdd:PLN02387 607 QALEKWAKKAGIDYSNFAELCekeeAVKEV-QQSLSKAAKAARLEKF 652
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
211-575 |
1.54e-08 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 57.44 E-value: 1.54e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 211 IIYTSGTTGRPKGVL-STHENVqavtTGLVEKWEWKKEDVILHVLPLHH------VHGVINKLLCplwVGATCVML---- 279
Cdd:PTZ00237 259 ILYTSGTTGNSKAVVrSNGPHL----VGLKYYWRSIIEKDIPTVVFSHSsigwvsFHGFLYGSLS---LGNTFVMFeggi 331
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 280 --PEFNAQMVWKKLlssQAPRISVFMAVPTIYAKLVEYydkhffQPQVQDFVRAFCQENIRLMVSGSAALPVPVLE---- 353
Cdd:PTZ00237 332 ikNKHIEDDLWNTI---EKHKVTHTLTLPKTIRYLIKT------DPEATIIRSKYDLSNLKEIWCGGEVIEESIPEyien 402
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 354 --KWKSITGhtllerYGMTEIGMALSNPLHGVRIP-GSVGTPLPGVEVCIATEtlkdgarsytihaQGDESNtqvtpglE 430
Cdd:PTZ00237 403 klKIKSSRG------YGQTEIGITYLYCYGHINIPyNATGVPSIFIKPSILSE-------------DGKELN-------V 456
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 431 GQEGEL---LVRGPSVFREYWNRPRETEDAFTP-DGWFKTGDTAaYKD--GVYWIKGRtSVDIIKNGGFKISALEVERHL 504
Cdd:PTZ00237 457 NEIGEVafkLPMPPSFATTFYKNDEKFKQLFSKfPGYYNSGDLG-FKDenGYYTIVSR-SDDQIKISGNKVQLNTIETSI 534
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2452540125 505 LAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLSVKDLKDWA-------RDTMAPYTIPTELIVVEEIPRNQMGKV 575
Cdd:PTZ00237 535 LKHPLVLECCSIGIYDPDCYNVPIGLLVLKQDQSNQSIDLNKLKneinniiTQDIESLAVLRKIIIVNQLPKTKTGKI 612
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
214-537 |
1.36e-07 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 54.39 E-value: 1.36e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 214 TSGTTGRPKGVLSTHENVQAVTTGLVEKWEW-KKEDVILHVLPLHHVHGVINkLLCPLWVGATCVMLPE--FNAQ-MVWK 289
Cdd:PRK05851 160 TAGSTGTPRTAILSPGAVLSNLRGLNARVGLdAATDVGCSWLPLYHDMGLAF-LLTAALAGAPLWLAPTtaFSASpFRWL 238
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 290 KLLS-SQAprisVFMAVPTIYAKLVEYYDKHffqpqvqdfVRAFCQENIRLMVSGSAALPVPVLEKWKSITGH------T 362
Cdd:PRK05851 239 SWLSdSRA----TLTAAPNFAYNLIGKYARR---------VSDVDLGALRVALNGGEPVDCDGFERFATAMAPfgfdagA 305
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 363 LLERYGMTEIGMALSNPLHGV---------------RIPGSVGTPLPGVEVCIATetlkdgarsytihaqGDESntqvtP 427
Cdd:PRK05851 306 AAPSYGLAESTCAVTVPVPGIglrvdevttddgsgaRRHAVLGNPIPGMEVRISP---------------GDGA-----A 365
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 428 GLEGQE-GELLVRGPSVFREYWNrpretEDAFTPDGWFKTGDTAAYKDGVYWIKGRTSvDIIKNGGFKISALEVERhlla 506
Cdd:PRK05851 366 GVAGREiGEIEIRGASMMSGYLG-----QAPIDPDDWFPTGDLGYLVDGGLVVCGRAK-ELITVAGRNIFPTEIER---- 435
|
330 340 350
....*....|....*....|....*....|.
gi 2452540125 507 hphitdVAVigppdMVWGQRVSAVVQLRKGE 537
Cdd:PRK05851 436 ------VAA-----QVRGVREGAVVAVGTGE 455
|
|
| A_NRPS_alphaAR |
cd17647 |
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ... |
445-580 |
1.68e-07 |
|
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.
Pssm-ID: 341302 [Multi-domain] Cd Length: 520 Bit Score: 54.06 E-value: 1.68e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 445 REYWNRPRetedaftpDGWFKTGDTAAY-KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHI------------- 510
Cdd:cd17647 363 RQFWLGPR--------DRLYRTGDLGRYlPNGDCECCGRAD-DQVKIRGFRIELGEIDTHISQHPLVrenitlvrrdkde 433
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 511 --TDVAVIGPPDMVWGQRVSA------------VVQLRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPRNQMGKVN 576
Cdd:cd17647 434 epTLVSYIVPRFDKPDDESFAqedvpkevstdpIVKGLIGYRKLIKDIREFLKKRLASYAIPSLIVVLDKLPLNPNGKVD 513
|
....
gi 2452540125 577 KKEL 580
Cdd:cd17647 514 KPKL 517
|
|
| PTZ00342 |
PTZ00342 |
acyl-CoA synthetase; Provisional |
211-468 |
2.77e-07 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240370 [Multi-domain] Cd Length: 746 Bit Score: 53.57 E-value: 2.77e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 211 IIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHV--LPLHHVHGVINKLLCpLWVGATCvmlpefnaqMVW 288
Cdd:PTZ00342 309 IVYTSGTSGKPKGVMLSNKNLYNTVVPLCKHSIFKKYNPKTHLsyLPISHIYERVIAYLS-FMLGGTI---------NIW 378
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 289 KK--------LLSSQApriSVFMAVPT----IYAKLVEYYD-----KHFFQPQVQDFVRAF------------------- 332
Cdd:PTZ00342 379 SKdinyfskdIYNSKG---NILAGVPKvfnrIYTNIMTEINnlpplKRFLVKKILSLRKSNnnggfskflegithisski 455
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 333 ---CQENIRLMVSGSAALPVPVLEKWKSITGHTLLERYGMTEIGMALSNPLHGVRIPGSVGTPL-PGVEVCIAT-ETLKd 407
Cdd:PTZ00342 456 kdkVNPNLEVILNGGGKLSPKIAEELSVLLNVNYYQGYGLTETTGPIFVQHADDNNTESIGGPIsPNTKYKVRTwETYK- 534
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2452540125 408 garsytihaqgdesNTQVTPglegqEGELLVRGPSVFREYWNRPRETEDAFTPDGWFKTGD 468
Cdd:PTZ00342 535 --------------ATDTLP-----KGELLIKSDSIFSGYFLEKEQTKNAFTEDGYFKTGD 576
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
194-580 |
5.58e-07 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 52.43 E-value: 5.58e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 194 EEGPLTTCSSWKNRgAMIIYTSGTTGRPKGVLSTHENVQAVTTGLVEKWEWKKEDVILHVLPLHHVHGVINKLLCPLWVG 273
Cdd:cd05939 93 TEPPSQDDVNFRDK-LFYIYTSGTTGLPKAAVIVHSRYYRIAAGAYYAFGMRPEDVVYDCLPLYHSAGGIMGVGQALLHG 171
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 274 ATCVMLPEFNAQMVWKKLLSSQAPrISVFMAVPTIY---AKLVEYYDKHffqpqvqdfvrafcqeNIRLMVsGSAALPvp 350
Cdd:cd05939 172 STVVIRKKFSASNFWDDCVKYNCT-IVQYIGEICRYllaQPPSEEEQKH----------------NVRLAV-GNGLRP-- 231
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 351 vlEKWKSITGH----TLLERYGMTEIGMALSNPLHGV-------RIPGSVgTPLPGVEVCIATETLKDGARSYTIHAQGD 419
Cdd:cd05939 232 --QIWEQFVRRfgipQIGEFYGATEGNSSLVNIDNHVgacgfnsRILPSV-YPIRLIKVDEDTGELIRDSDGLCIPCQPG 308
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 420 ESNTQVtpglegqeGELLVRGPSV-FREYWNRpRETE-----DAFTP-DGWFKTGDTAAYKD-GVYWIKGRTSvDIIKNG 491
Cdd:cd05939 309 EPGLLV--------GKIIQNDPLRrFDGYVNE-GATNkkiarDVFKKgDSAFLSGDVLVMDElGYLYFKDRTG-DTFRWK 378
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 492 GFKISALEVERHLLAHPHITDVAVIGP--PDMVWGQRVSAVVqlRKGEMLSVKDLKDWARDTMAPYTIPTELIVVEEIPR 569
Cdd:cd05939 379 GENVSTTEVEGILSNVLGLEDVVVYGVevPGVEGRAGMAAIV--DPERKVDLDRFSAVLAKSLPPYARPQFIRLLPEVDK 456
|
410
....*....|.
gi 2452540125 570 NQMGKVNKKEL 580
Cdd:cd05939 457 TGTFKLQKTDL 467
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
130-574 |
1.03e-06 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 51.50 E-value: 1.03e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 130 IAVPLY----KKHPVTEleyVIQDSQSALVIAAEEYVGKITPSAEKLGVPVLPLLRSCSDGSASHPAVEEGPLTTCSS-- 203
Cdd:cd05943 175 FAVDAYtyngKRHDVRE---KVAELVKGLPSLLAVVVVPYTVAAGQPDLSKIAKALTLEDFLATGAAGELEFEPLPFDhp 251
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 204 -WknrgamIIYTSGTTGRPKGVLSTHENVqavttgLVEKWewkKEdvilhvlplHHVHGVI-----------------NK 265
Cdd:cd05943 252 lY------ILYSSGTTGLPKCIVHGAGGT------LLQHL---KE---------HILHCDLrpgdrlfyyttcgwmmwNW 307
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 266 LLCPLWVGATCVML------PEFNAQmvWKklLSSQApRISVFMAvptiYAKLVEYYDKHFFQPqvqdfVRAFCQENIRL 339
Cdd:cd05943 308 LVSGLAVGATIVLYdgspfyPDTNAL--WD--LADEE-GITVFGT----SAKYLDALEKAGLKP-----AETHDLSSLRT 373
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 340 MVSGSAALPVP----VLEKWK------SITGHTllerygmtEI--GMALSNPLHGVRiPGSVGTPLPGVEVCIAtetlkd 407
Cdd:cd05943 374 ILSTGSPLKPEsfdyVYDHIKpdvllaSISGGT--------DIisCFVGGNPLLPVY-RGEIQCRGLGMAVEAF------ 438
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 408 garsytihaqgDESNTQVTpgleGQEGELLVRG--PSVFREYWNRPRET--EDAF--TPDGWFKTGDTA-AYKDGVYWIK 480
Cdd:cd05943 439 -----------DEEGKPVW----GEKGELVCTKpfPSMPVGFWNDPDGSryRAAYfaKYPGVWAHGDWIeITPRGGVVIL 503
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2452540125 481 GRtSVDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEMLS---VKDLKDWARDTMAPYTI 557
Cdd:cd05943 504 GR-SDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDERVILFVKLREGVELDdelRKRIRSTIRSALSPRHV 582
|
490
....*....|....*..
gi 2452540125 558 PTELIVVEEIPRNQMGK 574
Cdd:cd05943 583 PAKIIAVPDIPRTLSGK 599
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
473-539 |
2.12e-05 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 47.25 E-value: 2.12e-05
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2452540125 473 KDGVYWIKGRTSvDIIKNGGFKISALEVERHLLAHPHITDVAVIGPPDMVWGQRVSAVVQLRKGEML 539
Cdd:PRK10524 485 ADGYYFILGRTD-DVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPKDSDSL 550
|
|
| |
