Serine Recombinase family, catalytic domain; a DNA binding domain may be present either N- or C-terminal to the catalytic domain. These enzymes perform site-specific recombination of DNA molecules by a concerted, four-strand cleavage and rejoining mechanism which involves a transient phosphoserine linkage between DNA and serine recombinase. Serine recombinases demonstrate functional versatility and include resolvases, invertases, integrases, and transposases. Resolvases and invertases (i.e. Tn3, gamma-delta, Tn5044 resolvases, Gin and Hin invertases) in this family contain a C-terminal DNA binding domain and comprise a major phylogenic group. Also included are phage- and bacterial-encoded recombinases such as phiC31 integrase, SpoIVCA excisionase, and Tn4451 TnpX transposase. These integrases and transposases have larger C-terminal domains compared to resolvases/invertases and are referred to as large serine recombinases. Also belonging to this family are proteins with N-terminal DNA binding domains similar to IS607- and IS1535-transposases from Helicobacter and Mycobacterium.

                          10        20
                  ....*....|....*....|....*
gi 2734514876   3 LFGYARVSTSQQSLDLQVRALKDAG 27
Cdd:cd03768     1 VIGYARVSTDDQSLERQLEALKAAG 25

Serine Recombinase (SR) family, Resolvase and Invertase subfamily, catalytic domain; members contain a C-terminal DNA binding domain. Serine recombinases catalyze site-specific recombination of DNA molecules by a concerted, four-strand cleavage and rejoining mechanism which involves a transient phosphoserine linkage between DNA and the enzyme. They are functionally versatile and include resolvases, invertases, integrases, and transposases. Resolvases and invertases affect resolution or inversion and comprise a major phylogenic group. Resolvases (e.g. Tn3, gamma-delta, and Tn5044) normally recombine two sites in direct repeat causing deletion of the DNA between the sites. Invertases (e.g. Gin and Hin) recombine sites in inverted repeat to invert the DNA between the sites. Cointegrate resolution with gamma-delta resolvase requires the formation of a synaptosome of three resolvase dimers bound to each of two res sites on the DNA. Also included in this subfamily are some putative integrases including a sequence from bacteriophage phi-FC1.

                          10        20
                  ....*....|....*....|....*
gi 2734514876   3 LFGYARVSTSQQSLDLQVRALKDAG 27
Cdd:cd03768     1 VIGYARVSTDDQSLERQLEALKAAG 25

fdxN element excision recombinase XisF;

                          10        20
                  ....*....|....*....|....*...
gi 2734514876   5 GYARVSTSQQ-----SLDLQVRALKDAG 27
Cdd:NF041201    3 GYARVSTREQaddghALEQQIARLKKAG 30

Resolvase, N terminal domain; The N-terminal domain of the resolvase family contains the active site and the dimer interface. The extended arm at the C-terminus of this domain connects to the C-terminal helix-turn-helix domain of resolvase.

                           10        20
                   ....*....|....*....|....*.
gi 2734514876    4 FGYARVSTSQQ---SLDLQVRALKDA 26
Cdd:smart00857   2 AGYARVSTDDQadgSLERQLEALRAY 27

Serine Recombinase (SR) family, Resolvase and Invertase subfamily, catalytic domain; members contain a C-terminal DNA binding domain. Serine recombinases catalyze site-specific recombination of DNA molecules by a concerted, four-strand cleavage and rejoining mechanism which involves a transient phosphoserine linkage between DNA and the enzyme. They are functionally versatile and include resolvases, invertases, integrases, and transposases. Resolvases and invertases affect resolution or inversion and comprise a major phylogenic group. Resolvases (e.g. Tn3, gamma-delta, and Tn5044) normally recombine two sites in direct repeat causing deletion of the DNA between the sites. Invertases (e.g. Gin and Hin) recombine sites in inverted repeat to invert the DNA between the sites. Cointegrate resolution with gamma-delta resolvase requires the formation of a synaptosome of three resolvase dimers bound to each of two res sites on the DNA. Also included in this subfamily are some putative integrases including a sequence from bacteriophage phi-FC1.

                          10        20
                  ....*....|....*....|....*
gi 2734514876   3 LFGYARVSTSQQSLDLQVRALKDAG 27
Cdd:cd03768     1 VIGYARVSTDDQSLERQLEALKAAG 25

fdxN element excision recombinase XisF;

                          10        20
                  ....*....|....*....|....*...
gi 2734514876   5 GYARVSTSQQ-----SLDLQVRALKDAG 27
Cdd:NF041201    3 GYARVSTREQaddghALEQQIARLKKAG 30

Resolvase, N terminal domain; The N-terminal domain of the resolvase family contains the active site and the dimer interface. The extended arm at the C-terminus of this domain connects to the C-terminal helix-turn-helix domain of resolvase.

                           10        20
                   ....*....|....*....|....*.
gi 2734514876    4 FGYARVSTSQQ---SLDLQVRALKDA 26
Cdd:smart00857   2 AGYARVSTDDQadgSLERQLEALRAY 27