NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|2556333338|ref|WP_304096281|]
View 

MULTISPECIES: GNAT family N-acetyltransferase [Methanobrevibacter]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11441181)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
2-99 4.10e-28

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


:

Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 99.68  E-value: 4.10e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338   2 AVEKDKVVGWIALSHTLSRATYSGSVEVRIYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAAIFSINVVSIKFHEKWG 81
Cdd:COG1247    57 AEEDGEVVGFASLGPFRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLG 136

                          90
                  ....*....|....*...
gi 2556333338  82 FRKIRYRERIDKdKFGKW 99
Cdd:COG1247   137 FEEVGTLPEVGF-KFGRW 153
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
2-99 4.10e-28

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 99.68  E-value: 4.10e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338   2 AVEKDKVVGWIALSHTLSRATYSGSVEVRIYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAAIFSINVVSIKFHEKWG 81
Cdd:COG1247    57 AEEDGEVVGFASLGPFRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLG 136

                          90
                  ....*....|....*...
gi 2556333338  82 FRKIRYRERIDKdKFGKW 99
Cdd:COG1247   137 FEEVGTLPEVGF-KFGRW 153
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 1-82 2.23e-12

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 58.30  E-value: 2.23e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338   1 MAVEKDKVVGWIALSHTLSRATYSgsVEVRIYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAAIFSINVVSIKFHEKW 80
Cdd:pfam00583  37 VAEEDGELVGFASLSIIDDEPPVG--EIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLAAIALYEKL 114


                  ..
gi 2556333338  81 GF 82
Cdd:pfam00583 115 GF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 2-63 2.90e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.41  E-value: 2.90e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2556333338  2 AVEKDKVVGWIALSHTLSRATYsGSVEvRIYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLY 63
Cdd:cd04301    4 AEDDGEIVGFASLSPDGSGGDT-AYIG-DLAVLPEYRGKGIGSALLEAAEEEARERGAKRLR 63
rimI PRK09491
ribosomal-protein-alanine N-acetyltransferase; Provisional
 31-88 2.38e-04

ribosomal-protein-alanine N-acetyltransferase; Provisional


Pssm-ID: 181904 [Multi-domain]  Cd Length: 146  Bit Score: 37.60  E-value: 2.38e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2556333338  31 IYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAAIFSINVVSIKFHEKWGFRKIRYR 88
Cdd:PRK09491   69 IAVDPDYQRQGLGRALLEHLIDELEKRGVATLWLEVRASNAAAIALYESLGFNEVTIR 126
 
Name Accession Description Interval E-value
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
2-99 4.10e-28

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 99.68  E-value: 4.10e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338   2 AVEKDKVVGWIALSHTLSRATYSGSVEVRIYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAAIFSINVVSIKFHEKWG 81
Cdd:COG1247    57 AEEDGEVVGFASLGPFRPRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLG 136

                          90
                  ....*....|....*...
gi 2556333338  82 FRKIRYRERIDKdKFGKW 99
Cdd:COG1247   137 FEEVGTLPEVGF-KFGRW 153
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 1-82 2.23e-12

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 58.30  E-value: 2.23e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338   1 MAVEKDKVVGWIALSHTLSRATYSgsVEVRIYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAAIFSINVVSIKFHEKW 80
Cdd:pfam00583  37 VAEEDGELVGFASLSIIDDEPPVG--EIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLAAIALYEKL 114


                  ..
gi 2556333338  81 GF 82
Cdd:pfam00583 115 GF 116
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 2-96 3.48e-10

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 52.75  E-value: 3.48e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338   2 AVEKDKVVGWIALSHTlsratYSGSVEV-RIYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAAIFSINVVSIKFHEKW 80
Cdd:COG0454    39 VDDKGEPIGFAGLRRL-----DDKVLELkRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERL 113

                          90
                  ....*....|....*.
gi 2556333338  81 GFRKIRYRERIDKDKF 96
Cdd:COG0454   114 GFKEIERYVAYVGGEF 129
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 30-94 5.97e-10

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 51.19  E-value: 5.97e-10
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2556333338 30 RIYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAAIFSINVVSIKFHEKWGFRKIRYRERIDKD 94
Cdd:COG0456   18 DLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNYYGD 82
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
2-86 7.37e-09

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 49.70  E-value: 7.37e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338   2 AVEKDKVVGWIALShtlsRATYSGSVEV----RIYVDDSFKQKGIGYKLMQEIIRIAPKKGiwslYAAIF-SINVVSIKF 76
Cdd:COG3153    44 AEDDGEIVGHVALS----PVDIDGEGPAlllgPLAVDPEYRGQGIGRALMRAALEAARERG----ARAVVlLGDPSLLPF 115

                          90
                  ....*....|
gi 2556333338  77 HEKWGFRKIR 86
Cdd:COG3153   116 YERFGFRPAG 125
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 2-84 5.76e-08

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 45.91  E-value: 5.76e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338  2 AVEKDKVVGWIALSHTLSRATYsgsVEVRIYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAaifSINVVSIKFHEKWG 81
Cdd:pfam13508  8 AEDDGKIVGFAALLPLDDEGAL---AELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLEL---ETTNRAAAFYEKLG 81


                 ...
gi 2556333338 82 FRK 84
Cdd:pfam13508 82 FEE 84
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 4-99 4.20e-07

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 45.38  E-value: 4.20e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338   4 EKDKVVGWIALSHtlsRATYSGSVEVRIYVDDSFKQKGIGYKLMQEIIRIAPKK-GIWSLYAAIFSINVVSIKFHEKWGF 82
Cdd:COG1670    69 EDGELIGVVGLYD---IDRANRSAEIGYWLAPAYWGKGYATEALRALLDYAFEElGLHRVEAEVDPDNTASIRVLEKLGF 145

                          90
                  ....*....|....*..
gi 2556333338  83 RKIRYRERIDKDKfGKW 99
Cdd:COG1670   146 RLEGTLRDALVID-GRY 161
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 2-85 8.96e-07

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 43.83  E-value: 8.96e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338   2 AVEKDKVVGWIALshtlsRATYSGSVEVR-IYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAAIFSinvVSIKFHEKW 80
Cdd:COG1246    33 AEEDGEIVGCAAL-----HPLDEDLAELRsLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLTTS---AAIHFYEKL 104


                  ....*
gi 2556333338  81 GFRKI 85
Cdd:COG1246   105 GFEEI 109
Acetyltransf_4 pfam13420
Acetyltransferase (GNAT) domain;
1-99 1.17e-05

Acetyltransferase (GNAT) domain;


Pssm-ID: 433192 [Multi-domain]  Cd Length: 153  Bit Score: 41.20  E-value: 1.17e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338   1 MAVEKDKVVGWIALSHTlsRATYSGSVEVRIYVDDSFKqKGIGYKLMQEIIRIAPKK-GIWSLYAAIFSINVVSIKFHEK 79
Cdd:pfam13420  53 GVAESDRLIGYATLRQF--DYVKTHKAELSFYVVKNND-EGINRELINAIIQYARKNqNIENLEACIASNNINAIVFLKA 129

                          90       100
                  ....*....|....*....|
gi 2556333338  80 WGFRKIRYRERIDKdKFGKW 99
Cdd:pfam13420 130 IGFEWLGIERNAIK-KNGRW 148
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 2-63 2.90e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.41  E-value: 2.90e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2556333338  2 AVEKDKVVGWIALSHTLSRATYsGSVEvRIYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLY 63
Cdd:cd04301    4 AEDDGEIVGFASLSPDGSGGDT-AYIG-DLAVLPEYRGKGIGSALLEAAEEEARERGAKRLR 63
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 2-85 3.35e-05

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 39.56  E-value: 3.35e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2556333338   2 AVEKDKVVGWIALShtlsratySGSVEVRIYVDDSFKQKGIGYKLMQEIIRIAPKKGIwslYAAIFSIN--VVSIKFHEK 79
Cdd:pfam13673  36 AFEGGQIVGVIALR--------DRGHISLLFVDPDYQGQGIGKALLEAVEDYAEKDGI---KLSELTVNasPYAVPFYEK 104


                  ....*.
gi 2556333338  80 WGFRKI 85
Cdd:pfam13673 105 LGFRAT 110
rimI PRK09491
ribosomal-protein-alanine N-acetyltransferase; Provisional
 31-88 2.38e-04

ribosomal-protein-alanine N-acetyltransferase; Provisional


Pssm-ID: 181904 [Multi-domain]  Cd Length: 146  Bit Score: 37.60  E-value: 2.38e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2556333338  31 IYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAAIFSINVVSIKFHEKWGFRKIRYR 88
Cdd:PRK09491   69 IAVDPDYQRQGLGRALLEHLIDELEKRGVATLWLEVRASNAAAIALYESLGFNEVTIR 126
FR47 pfam08445
FR47-like protein; The members of this family are similar to the C-terminal region of the D. ...
 31-91 2.54e-04

FR47-like protein; The members of this family are similar to the C-terminal region of the D. melanogaster hypothetical protein FR47. This protein has been found to consist of two N-acyltransferase-like domains swapped with the C-terminal strands.


Pssm-ID: 117022 [Multi-domain]  Cd Length: 86  Bit Score: 36.54  E-value: 2.54e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2556333338 31 IYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLyAAIFSINVVSIKFHEKWGFRKIRYRERI 91
Cdd:pfam08445 27 LQTLPEHRRRGLGSRLVAALARGIAERGITPF-AVVVAGNTPSRRLYEKLGFRKIDETYWV 86
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
30-85 3.19e-04

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 37.09  E-value: 3.19e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2556333338  30 RIYVDDSFKQKGIGYKLMQEIIRIAPKKG---IW---SLYAaifsinvvsIKFHEKWGFRKI 85
Cdd:COG2153    63 RVAVLPEYRGQGLGRALMEAAIEEARERGarrIVlsaQAHA---------VGFYEKLGFVPV 115
PTZ00330 PTZ00330
acetyltransferase; Provisional
 25-92 4.61e-04

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 36.74  E-value: 4.61e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2556333338  25 GSVEvRIYVDDSFKQKGIGYKLMQEIIRIAPKKGIwslYAAIFSINVVSIKFHEKWGFRKIRYRERID 92
Cdd:PTZ00330   83 GHIE-DVVVDPSYRGQGLGRALISDLCEIARSSGC---YKVILDCTEDMVAFYKKLGFRACERQMRLD 146
COG3818 COG3818
Predicted N-acetyltransferase, GNAT superfamily [General function prediction only];
30-85 1.08e-03

Predicted N-acetyltransferase, GNAT superfamily [General function prediction only];


Pssm-ID: 443030 [Multi-domain]  Cd Length: 168  Bit Score: 36.07  E-value: 1.08e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2556333338  30 RIYVDDSFKQKGIGYKLMQEIIRIAPKKGIWSLYAAIFSI--NVVSIKFHEKWGFRKI 85
Cdd:COG3818    89 RIVVAPSARGRGLGRALYADVFSYARARGVPRVTCEVNLEppNPGSLAFHARLGFREV 146
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH