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Conserved domains on  [gi|2521008029|ref|WP_285813507|]
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MULTISPECIES: N-acetyltransferase [Staphylococcus]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11418877)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 5-88 8.23e-17

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 68.53  E-value: 8.23e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2521008029  5 EGDLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQGYQFIGETGQ-GDYQYYKM 83
Cdd:COG0456    8 VDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNyYGDDALVM 87


                 ....*
gi 2521008029 84 RKSLK 88
Cdd:COG0456   88 EKELA 92
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 5-88 8.23e-17

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 68.53  E-value: 8.23e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2521008029  5 EGDLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQGYQFIGETGQ-GDYQYYKM 83
Cdd:COG0456    8 VDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNyYGDDALVM 87


                 ....*
gi 2521008029 84 RKSLK 88
Cdd:COG0456   88 EKELA 92
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 2-67 2.96e-13

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 60.22  E-value: 2.96e-13
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2521008029   2 NLDEGDLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQGY 67
Cdd:pfam00583  51 SIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLAAIALYEKLGF 116
rimI TIGR01575
ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related ...
 7-72 6.65e-10

ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related N-acetyltransferase (GNAT) superfamily. This model covers prokarotes and the archaea. The seed contains a characterized accession for Gram negative E. coli. An untraceable characterized accession (PIR|S66013) for Gram positive B. subtilis scores well (205.0) in the full alignment. Characterized members are lacking in the archaea. Noise cutoff (72.4) was set to exclude M. loti paralog of rimI. Trusted cutoff (80.0) was set at next highest scoring member in the mini-database. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 273701 [Multi-domain]  Cd Length: 131  Bit Score: 51.95  E-value: 6.65e-10
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2521008029   7 DLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQGYQFIGE 72
Cdd:TIGR01575  51 VLDEAHILNIAVKPEYQGQGIGRALLRELIDEAKGRGVNEIFLEVRVSNIAAQALYKKLGFNEIAI 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 4-50 1.58e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 44.19  E-value: 1.58e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 2521008029  4 DEGDLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLT 50
Cdd:cd04301   19 DGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
PRK10975 PRK10975
dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;
9-66 3.60e-06

dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;


Pssm-ID: 182877  Cd Length: 194  Bit Score: 42.61  E-value: 3.60e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2521008029   9 NEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQG 66
Cdd:PRK10975  125 TDARIGLLAVFPGAQGRGIGARLMQAALNWCQARGLTRLRVATQMGNLAALRLYIRSG 182
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 5-88 8.23e-17

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 68.53  E-value: 8.23e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2521008029  5 EGDLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQGYQFIGETGQ-GDYQYYKM 83
Cdd:COG0456    8 VDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNyYGDDALVM 87


                 ....*
gi 2521008029 84 RKSLK 88
Cdd:COG0456   88 EKELA 92
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 12-89 9.58e-15

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 64.30  E-value: 9.58e-15
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2521008029  12 HVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQGYQFIGEtgQGDYQYYKMRKSLKL 89
Cdd:COG0454    60 ELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERLGFKEIER--YVAYVGGEFEKELSL 135
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
12-85 2.10e-14

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 62.23  E-value: 2.10e-14
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2521008029 12 HVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQGYQFIGEtgqgdYQYYKMRK 85
Cdd:COG3393   17 EISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAARRLYERLGFRPVGE-----YATVLFRK 85
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 2-67 2.96e-13

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 60.22  E-value: 2.96e-13
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2521008029   2 NLDEGDLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQGY 67
Cdd:pfam00583  51 SIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAADNLAAIALYEKLGF 116
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
7-87 3.78e-13

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 60.20  E-value: 3.78e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2521008029   7 DLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEkvpAQRLYKKQGYQFIGET-GQGDYQYYKMRK 85
Cdd:COG2153    55 GDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRIVLSAQAH---AVGFYEKLGFVPVGEEfLEAGIPHIDMRK 131


                  ..
gi 2521008029  86 SL 87
Cdd:COG2153   132 PL 133
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 12-87 2.58e-11

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 55.35  E-value: 2.58e-11
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2521008029  12 HVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDvEKVPAQRLYKKQGYQFIGETGQGD-YQYYKMRKSL 87
Cdd:pfam13673  53 HISLLFVDPDYQGQGIGKALLEAVEDYAEKDGIKLSELTVN-ASPYAVPFYEKLGFRATGPEQEFNgIRFVPMEKEL 128
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
5-87 1.14e-10

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 53.94  E-value: 1.14e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2521008029   5 EGDLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPaqrLYKKQGYQFIGETGQGDYQY-YKM 83
Cdd:COG3153    62 DGEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLP---FYERFGFRPAGELGLTLGPDeVFL 138


                  ....
gi 2521008029  84 RKSL 87
Cdd:COG3153   139 AKEL 142
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 12-73 1.33e-10

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 53.46  E-value: 1.33e-10
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2521008029  12 HVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDvekVPAQRLYKKQGYQFIGET 73
Cdd:COG1246    54 ELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLTT---SAAIHFYEKLGFEEIDKE 112
rimI TIGR01575
ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related ...
 7-72 6.65e-10

ribosomal-protein-alanine acetyltransferase; Members of this model belong to the GCN5-related N-acetyltransferase (GNAT) superfamily. This model covers prokarotes and the archaea. The seed contains a characterized accession for Gram negative E. coli. An untraceable characterized accession (PIR|S66013) for Gram positive B. subtilis scores well (205.0) in the full alignment. Characterized members are lacking in the archaea. Noise cutoff (72.4) was set to exclude M. loti paralog of rimI. Trusted cutoff (80.0) was set at next highest scoring member in the mini-database. [Protein synthesis, Ribosomal proteins: synthesis and modification]


Pssm-ID: 273701 [Multi-domain]  Cd Length: 131  Bit Score: 51.95  E-value: 6.65e-10
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2521008029   7 DLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQGYQFIGE 72
Cdd:TIGR01575  51 VLDEAHILNIAVKPEYQGQGIGRALLRELIDEAKGRGVNEIFLEVRVSNIAAQALYKKLGFNEIAI 116
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 5-68 1.27e-09

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 50.15  E-value: 1.27e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2521008029  5 EGDLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDvekVPAQRLYKKQGYQ 68
Cdd:pfam13508 23 LDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETT---NRAAAFYEKLGFE 83
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
20-87 2.22e-08

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 48.45  E-value: 2.22e-08
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2521008029  20 ESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQGYQFIGETGQGDYQYYK------MRKSL 87
Cdd:COG1247    90 PDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLGFEEVGTLPEVGFKFGRwldlvlMQKRL 163
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 4-50 1.58e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 44.19  E-value: 1.58e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 2521008029  4 DEGDLNEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLT 50
Cdd:cd04301   19 DGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
PRK10975 PRK10975
dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;
9-66 3.60e-06

dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;


Pssm-ID: 182877  Cd Length: 194  Bit Score: 42.61  E-value: 3.60e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2521008029   9 NEYHVSMLATLESYRGNGLGQKLLTYAEQLALNKNFTHISLTVDVEKVPAQRLYKKQG 66
Cdd:PRK10975  125 TDARIGLLAVFPGAQGRGIGARLMQAALNWCQARGLTRLRVATQMGNLAALRLYIRSG 182
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 20-72 9.86e-05

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 38.83  E-value: 9.86e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2521008029  20 ESYRGNGLG----QKLLTYA-EQLALNKnfthISLTVDVEKVPAQRLYKKQGYQFIGE 72
Cdd:COG1670    97 PAYWGKGYAtealRALLDYAfEELGLHR----VEAEVDPDNTASIRVLEKLGFRLEGT 150
PRK10562 PRK10562
putative acetyltransferase; Provisional
 22-79 1.06e-04

putative acetyltransferase; Provisional


Pssm-ID: 236715 [Multi-domain]  Cd Length: 145  Bit Score: 38.51  E-value: 1.06e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2521008029  22 YRGNGLGQKLLTYAEQLalnknFTHISLTVDVEKVPAQRLYKKQGYQFIGETGQGDYQ 79
Cdd:PRK10562   80 AVRRGIGKALMQHVQQR-----YPHLSLEVYQKNQRAVNFYHAQGFRIVDSAWQEETQ 132
PRK10514 PRK10514
putative acetyltransferase; Provisional
 1-76 6.09e-03

putative acetyltransferase; Provisional


Pssm-ID: 182510 [Multi-domain]  Cd Length: 145  Bit Score: 33.44  E-value: 6.09e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2521008029   1 MNLDEGdlneyHVSMLATLESYRGNGLGQKLLTYAeqLALnknftHISLTVDVEKVPAQRL--YKKQGYQFIGET---GQ 75
Cdd:PRK10514   65 MLLSGG-----HMEALFVDPDVRGCGVGRMLVEHA--LSL-----HPELTTDVNEQNEQAVgfYKKMGFKVTGRSevdDQ 132


                  .
gi 2521008029  76 G 76
Cdd:PRK10514  133 G 133
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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