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Conserved domains on  [gi|2520332465|ref|WP_285292569|]
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translesion error-prone DNA polymerase V subunit UmuC [Klebsiella variicola]

Protein Classification

DNA polymerase Y subunit UmuC family protein( domain architecture ID 1000143)

DNA polymerase Y subunit UmuC family protein similar to plasmid-encoded MucB protein, which is a DNA polymerase (pol RI) specialized for lesion bypass in the presence of MucA', RecA, and single strand-binding protein (SSB)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PolY super family cl28996
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ...
 1-420 0e+00

Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.


The actual alignment was detected with superfamily member PRK03609:

Pssm-ID: 452909 [Multi-domain]  Cd Length: 422  Bit Score: 765.85  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   1 MFALVDVNNFYTSCETVFRPDLKGKPIVVVSNNDGCIISRSAEAKSLGIKNGEPFFKIKNNPYYSSVHFFSSNYALYADL 80
Cdd:PRK03609    1 MFALCDVNSFYASCETVFRPDLRGKPVVVLSNNDGCVIARSAEAKALGIKMGDPWFKQKDLFRRCGVVCFSSNYELYADM 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  81 SDRVMQTLTAMSPSIEIYSIDEAFINLSGVMNCVPLSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKTW-RGT 159
Cdd:PRK03609   81 SNRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWqRQT 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 160 GGVVDLSNIVRQRRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFNVVLERTVRELRGEPCFELQEF 239
Cdd:PRK03609  161 GGVVDLSNLERQRKLLSLQPVEEVWGVGRRISKKLNAMGIKTALDLADTNIRFIRKHFNVVLERTVRELRGEPCLSLEEF 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 240 SPTKQQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPHAVNETYYGNQASMKLLTPSNDTR 319
Cdd:PRK03609  241 APTKQEIVCSRSFGERITDYESMRQAICSYAARAAEKLRGEHQYCRFISTFVKTSPFALNEPYYGNSASVKLLTPTQDSR 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 320 DIINCATNCLQRIWIDGYRYMKAGVMLADFFSNGIAQLNLFDDNAPRKNSAALMDVIDQLN-QSGKGKIWFAGQGIEKSW 398
Cdd:PRK03609  321 DIIAAATRALDAIWRDGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMKVLDTLNaKSGKGTLYFAGQGIAQQW 400

                         410       420
                  ....*....|....*....|..
gi 2520332465 399 AMKREMLSPAYTTRYSDLPVAK 420
Cdd:PRK03609  401 QMKREMLSPRYTTRWSDLLRVK 422
 
Name Accession Description Interval E-value
umuC PRK03609
translesion error-prone DNA polymerase V subunit UmuC;
1-420 0e+00

translesion error-prone DNA polymerase V subunit UmuC;


Pssm-ID: 179607 [Multi-domain]  Cd Length: 422  Bit Score: 765.85  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   1 MFALVDVNNFYTSCETVFRPDLKGKPIVVVSNNDGCIISRSAEAKSLGIKNGEPFFKIKNNPYYSSVHFFSSNYALYADL 80
Cdd:PRK03609    1 MFALCDVNSFYASCETVFRPDLRGKPVVVLSNNDGCVIARSAEAKALGIKMGDPWFKQKDLFRRCGVVCFSSNYELYADM 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  81 SDRVMQTLTAMSPSIEIYSIDEAFINLSGVMNCVPLSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKTW-RGT 159
Cdd:PRK03609   81 SNRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWqRQT 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 160 GGVVDLSNIVRQRRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFNVVLERTVRELRGEPCFELQEF 239
Cdd:PRK03609  161 GGVVDLSNLERQRKLLSLQPVEEVWGVGRRISKKLNAMGIKTALDLADTNIRFIRKHFNVVLERTVRELRGEPCLSLEEF 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 240 SPTKQQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPHAVNETYYGNQASMKLLTPSNDTR 319
Cdd:PRK03609  241 APTKQEIVCSRSFGERITDYESMRQAICSYAARAAEKLRGEHQYCRFISTFVKTSPFALNEPYYGNSASVKLLTPTQDSR 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 320 DIINCATNCLQRIWIDGYRYMKAGVMLADFFSNGIAQLNLFDDNAPRKNSAALMDVIDQLN-QSGKGKIWFAGQGIEKSW 398
Cdd:PRK03609  321 DIIAAATRALDAIWRDGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMKVLDTLNaKSGKGTLYFAGQGIAQQW 400

                         410       420
                  ....*....|....*....|..
gi 2520332465 399 AMKREMLSPAYTTRYSDLPVAK 420
Cdd:PRK03609  401 QMKREMLSPRYTTRWSDLLRVK 422
PolY_Pol_V_umuC cd01700
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ...
 3-348 0e+00

umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.


Pssm-ID: 176454 [Multi-domain]  Cd Length: 344  Bit Score: 512.09  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   3 ALVDVNNFYTSCETVFRPDLKGKPIVVVSNNDGCIISRSAEAKSLGIKNGEPFFKIKNNPYYSSVHFFSSNYALYADLSD 82
Cdd:cd01700     1 ALVDCNSFYASCERVFRPLLLGRPLVVLSNNDGCVIARSPEAKALGIKMGSPYFKVPDLLERHGVAVFSSNYALYGDMSR 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  83 RVMQTLTAMSPSIEIYSIDEAFINLSGVMNCVPLSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKTWRGTGGV 162
Cdd:cd01700    81 RIMSILERFSPDVEVYSIDESFLDLTGSLRFGDLEELARKIRRRILQETGIPVTVGIGPTKTLAKLANDLAKKKNPYGGV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 163 VDLSNIVRQRRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFNVVLERTVRELRGEPCFELQEFSPT 242
Cdd:cd01700   161 VDLTDEEVRDKLLKILPVGDVWGIGRRTAKKLNAMGIHTAGDLAQADPDLLRKKFGVVGERLVRELNGIDCLPLEEYPPP 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 243 KQQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPHAVNETYYgnQASMKLLTPSNDTRDII 322
Cdd:cd01700   241 KKSIGSSRSFGRDVTDLDELKQALAEYAERAAEKLRRQKSVARTISVFIGTSGFSRQPKYY--SATNTLPYPTNDTREIV 318

                         330       340
                  ....*....|....*....|....*.
gi 2520332465 323 NCATNCLQRIWIDGYRYMKAGVMLAD 348
Cdd:cd01700   319 KAALRLLYAIYRPGYAYRKAGVMLSD 344
DinP COG0389
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ...
 1-346 7.91e-128

Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];


Pssm-ID: 440158 [Multi-domain]  Cd Length: 336  Bit Score: 372.17  E-value: 7.91e-128
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   1 MFALVDVNNFYTSCETVFRPDLKGKPIVVVS-NNDGCIISRSAEAKSLGIKNGEPFFKIKN-NPyysSVHFFSSNYALYA 78
Cdd:COG0389     2 RILHVDMDAFYASVEQRDRPELRGKPVAVGGdNNRGVVAAASYEARAFGVRSGMPLFQARRlCP---DLVVLPPDFELYR 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  79 DLSDRVMQTLTAMSPSIEIYSIDEAFINLSGVMNCV-PLSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKTwr 157
Cdd:COG0389    79 DVSRRVMAILERYTPLVEPLSIDEAFLDVTGSARLFgSAEAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKP-- 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 158 gtGGVVDLSnIVRQRRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFNVVLERTVRELRGEPCFELQ 237
Cdd:COG0389   157 --DGLTVIP-PGEVAAFLAPLPVEKLWGVGPKTAEKLARLGIRTIGDLAALPRAELRRRFGKVGERLYRLARGIDPRPVE 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 238 EFSPTKqQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPHAVnetyygNQASMKLLTPSND 317
Cdd:COG0389   234 PRRPRK-SIGVERTFGEDLTDLEELEAALRRLAERLAERLRRQGLGARTVTVKLRTSDFRT------TTRSRTLPEPTDD 306

                         330       340
                  ....*....|....*....|....*....
gi 2520332465 318 TRDIINCATNCLQRIWIDGYRYMKAGVML 346
Cdd:COG0389   307 TAELLRAARELLERIYRPGRPVRLLGVRL 335
IMS pfam00817
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
 5-149 1.32e-38

impB/mucB/samB family; These proteins are involved in UV protection (Swiss).


Pssm-ID: 425885 [Multi-domain]  Cd Length: 148  Bit Score: 136.17  E-value: 1.32e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   5 VDVNNFYTSCETVFRPDLKGKPIVVVSNN-DGCIISRSAEAKSLGIKNGEPFFKI-KNNPyysSVHFFSSNYALYADLSD 82
Cdd:pfam00817   1 IDMDAFFASVELLRDPELKGKPVAVGGGNgRGIVAAASYEARKYGVRSGMPVFEAkKLCP---NLIVVPPDLELYRRASR 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2520332465  83 RVMQTLTAM-SPSIEIYSIDEAFINLSGVMNCVPL-STFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLA 149
Cdd:pfam00817  78 KIFEILRRFsTPKVEQASIDEAFLDLTGLEKLFGAeEALAKRLRREIAEETGLTCSIGIAPNKLLAKLA 146
 
Name Accession Description Interval E-value
umuC PRK03609
translesion error-prone DNA polymerase V subunit UmuC;
1-420 0e+00

translesion error-prone DNA polymerase V subunit UmuC;


Pssm-ID: 179607 [Multi-domain]  Cd Length: 422  Bit Score: 765.85  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   1 MFALVDVNNFYTSCETVFRPDLKGKPIVVVSNNDGCIISRSAEAKSLGIKNGEPFFKIKNNPYYSSVHFFSSNYALYADL 80
Cdd:PRK03609    1 MFALCDVNSFYASCETVFRPDLRGKPVVVLSNNDGCVIARSAEAKALGIKMGDPWFKQKDLFRRCGVVCFSSNYELYADM 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  81 SDRVMQTLTAMSPSIEIYSIDEAFINLSGVMNCVPLSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKTW-RGT 159
Cdd:PRK03609   81 SNRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWqRQT 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 160 GGVVDLSNIVRQRRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFNVVLERTVRELRGEPCFELQEF 239
Cdd:PRK03609  161 GGVVDLSNLERQRKLLSLQPVEEVWGVGRRISKKLNAMGIKTALDLADTNIRFIRKHFNVVLERTVRELRGEPCLSLEEF 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 240 SPTKQQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPHAVNETYYGNQASMKLLTPSNDTR 319
Cdd:PRK03609  241 APTKQEIVCSRSFGERITDYESMRQAICSYAARAAEKLRGEHQYCRFISTFVKTSPFALNEPYYGNSASVKLLTPTQDSR 320

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 320 DIINCATNCLQRIWIDGYRYMKAGVMLADFFSNGIAQLNLFDDNAPRKNSAALMDVIDQLN-QSGKGKIWFAGQGIEKSW 398
Cdd:PRK03609  321 DIIAAATRALDAIWRDGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMKVLDTLNaKSGKGTLYFAGQGIAQQW 400

                         410       420
                  ....*....|....*....|..
gi 2520332465 399 AMKREMLSPAYTTRYSDLPVAK 420
Cdd:PRK03609  401 QMKREMLSPRYTTRWSDLLRVK 422
PolY_Pol_V_umuC cd01700
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ...
 3-348 0e+00

umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.


Pssm-ID: 176454 [Multi-domain]  Cd Length: 344  Bit Score: 512.09  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   3 ALVDVNNFYTSCETVFRPDLKGKPIVVVSNNDGCIISRSAEAKSLGIKNGEPFFKIKNNPYYSSVHFFSSNYALYADLSD 82
Cdd:cd01700     1 ALVDCNSFYASCERVFRPLLLGRPLVVLSNNDGCVIARSPEAKALGIKMGSPYFKVPDLLERHGVAVFSSNYALYGDMSR 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  83 RVMQTLTAMSPSIEIYSIDEAFINLSGVMNCVPLSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKTWRGTGGV 162
Cdd:cd01700    81 RIMSILERFSPDVEVYSIDESFLDLTGSLRFGDLEELARKIRRRILQETGIPVTVGIGPTKTLAKLANDLAKKKNPYGGV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 163 VDLSNIVRQRRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFNVVLERTVRELRGEPCFELQEFSPT 242
Cdd:cd01700   161 VDLTDEEVRDKLLKILPVGDVWGIGRRTAKKLNAMGIHTAGDLAQADPDLLRKKFGVVGERLVRELNGIDCLPLEEYPPP 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 243 KQQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPHAVNETYYgnQASMKLLTPSNDTRDII 322
Cdd:cd01700   241 KKSIGSSRSFGRDVTDLDELKQALAEYAERAAEKLRRQKSVARTISVFIGTSGFSRQPKYY--SATNTLPYPTNDTREIV 318

                         330       340
                  ....*....|....*....|....*.
gi 2520332465 323 NCATNCLQRIWIDGYRYMKAGVMLAD 348
Cdd:cd01700   319 KAALRLLYAIYRPGYAYRKAGVMLSD 344
DinP COG0389
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ...
 1-346 7.91e-128

Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];


Pssm-ID: 440158 [Multi-domain]  Cd Length: 336  Bit Score: 372.17  E-value: 7.91e-128
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   1 MFALVDVNNFYTSCETVFRPDLKGKPIVVVS-NNDGCIISRSAEAKSLGIKNGEPFFKIKN-NPyysSVHFFSSNYALYA 78
Cdd:COG0389     2 RILHVDMDAFYASVEQRDRPELRGKPVAVGGdNNRGVVAAASYEARAFGVRSGMPLFQARRlCP---DLVVLPPDFELYR 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  79 DLSDRVMQTLTAMSPSIEIYSIDEAFINLSGVMNCV-PLSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKTwr 157
Cdd:COG0389    79 DVSRRVMAILERYTPLVEPLSIDEAFLDVTGSARLFgSAEAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKP-- 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 158 gtGGVVDLSnIVRQRRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFNVVLERTVRELRGEPCFELQ 237
Cdd:COG0389   157 --DGLTVIP-PGEVAAFLAPLPVEKLWGVGPKTAEKLARLGIRTIGDLAALPRAELRRRFGKVGERLYRLARGIDPRPVE 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 238 EFSPTKqQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPHAVnetyygNQASMKLLTPSND 317
Cdd:COG0389   234 PRRPRK-SIGVERTFGEDLTDLEELEAALRRLAERLAERLRRQGLGARTVTVKLRTSDFRT------TTRSRTLPEPTDD 306

                         330       340
                  ....*....|....*....|....*....
gi 2520332465 318 TRDIINCATNCLQRIWIDGYRYMKAGVML 346
Cdd:COG0389   307 TAELLRAARELLERIYRPGRPVRLLGVRL 335
PolY_Pol_IV_kappa cd03586
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ...
 5-335 9.78e-59

DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.


Pssm-ID: 176459 [Multi-domain]  Cd Length: 334  Bit Score: 195.05  E-value: 9.78e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   5 VDVNNFYTSCETVFRPDLKGKPIVVVSNNDGCIISrSA--EAKSLGIKNGEPFFKIKN-NPyyssvH--FFSSNYALYAD 79
Cdd:cd03586     3 IDMDAFYASVEQRDNPELKGKPVAVGGSSDRGVVS-TAsyEARKFGVRSAMPIFQAKKlCP-----NliFVPPRFDKYRE 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  80 LSDRVMQTLTAMSPSIEIYSIDEAFINLSGVMNCVPLST-FGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAK---- 154
Cdd:cd03586    77 VSRQIMEILREYTPLVEPLSIDEAYLDVTDYVRLFGSATeIAKEIRARIREETGLTASAGIAPNKFLAKIASDLNKpngl 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 155 TwrgtggVVDLSNIvrqRRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFNVVLERTVRELRGEPCF 234
Cdd:cd03586   157 T------VIPPEDV---EEFLAPLPVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVELLKKLFGKSGRRLYELARGIDNR 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 235 ELQEFSPTKqQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPHavnETYygnQASMKLLTP 314
Cdd:cd03586   228 PVEPDRERK-SIGVERTFSEDLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKLKYADF---STR---TRSRTLPEP 300

                         330       340
                  ....*....|....*....|.
gi 2520332465 315 SNDTRDIINCATNCLQRIWID 335
Cdd:cd03586   301 TDDAEDIYELALELLEELLDG 321
PolY cd00424
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ...
 5-346 1.03e-51

Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.


Pssm-ID: 176453 [Multi-domain]  Cd Length: 343  Bit Score: 176.78  E-value: 1.03e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   5 VDVNNFYTSCETVFRPDLKGKPIVVVSNND--GCIISRSAEAKSLGIKNGEPFFKIKNnpYYSSVHFFSSNYALYADLSD 82
Cdd:cd00424     3 IDFDNFFASVEQLARPELKGRPVVVVPFNSdsTCVIACSYEARKYGVKRGMPVREARK--MCPNLILVPARLDLYRRLSE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  83 RVMQTLTAMSPSIEIYSIDEAFINLSG-VMNCVPLSTFGHEIRTNVLKNTG-LTVGVGIAQTKTLAKLANHAAKTwrgtg 160
Cdd:cd00424    81 RLLSELEEVAPLVEVASIDELFLDLTGsARLLGLGSEVALRIKRHIAEQLGgITASIGIASNKLLAKLAAKYAKP----- 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 161 gvvDLSNIVRQ---RRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKH-FNVVLERTVRELRGEPCFEL 236
Cdd:cd00424   156 ---DGLTILDPedlPGFLSKLPLTDLPGIGAVTAKRLEAVGINPIGDLLAASPDALLALwGGVSGERLWYALRGIDDEPL 232

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 237 QEfSPTKQQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPHAVNETY--YGNQASmklLTP 314
Cdd:cd00424   233 SP-PRPRKSFSHERVLPRDSRNAEDARPLLRLLLEKLARRLRRDGRGATRLRLWLRTVDGRWSGHAdiPSRSAP---RPI 308

                         330       340       350
                  ....*....|....*....|....*....|....
gi 2520332465 315 SNDTRDIINCATNCLQRIWID--GYRYMKAGVML 346
Cdd:cd00424   309 STEDGELLHALDKLWRALLDDkgPRRLRRLGVRL 342
PRK03103 PRK03103
DNA polymerase IV; Reviewed
 4-390 1.57e-50

DNA polymerase IV; Reviewed


Pssm-ID: 235104 [Multi-domain]  Cd Length: 409  Bit Score: 175.58  E-value: 1.57e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   4 LVDVNNFYTSCETVFRPDLKGKPIVV---VSNNDGCIISRSAEAKSLGIKNGEPFFKIKNN-PyysSVHFFSSNYALYAD 79
Cdd:PRK03103    7 LVDMQSFYASVEKAANPELKGRPVIVsgdPERRSGVVLAACPLAKAYGVKTAERLWEAQQKcP---DLVVVKPRMQRYID 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  80 LSDRVMQTLTAMSPSIEIYSIDEAFINLSGVMNCV-PLSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLA-NHAAKtwR 157
Cdd:PRK03103   84 VSLQITRILEDFTDLVEPFSIDEQFLDVTGSQKLFgSPLEIAQKIQQRIMRETGVYARVGIGPNKLLAKMAcDNFAK--K 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 158 GTGGVVDLSNIVRQRRLLALvPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFNV---VLERTVRELRGEPcf 234
Cdd:PRK03103  162 NPDGLFTLDKEDVPADLWPL-PVRKLFGVGSRMEKHLRRMGIRTIGQLANTPLERLKKRWGIngeVLWRTANGIDYSP-- 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 235 eLQEFSPTKQqiicsRSFGNRVT------QYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTsphAVNETYYGNQAS 308
Cdd:PRK03103  239 -VTPHSLDRQ-----KAIGHQMTlprdyrGFEEIKVVLLELCEEVCRRARAKGYMGRTVSVSLRG---ADFDWPTGFSRQ 309

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 309 MKLLTPSNDTRDIINCATNCLQRIWiDGYRYMKAGVMLADFFSNGIAQLNLFDDNAPRKNSAALMDVIDqlNQSGKGKIW 388
Cdd:PRK03103  310 MTLPEPTNLAMEVYEAACKLFHRHW-DGKPVRRVGVTLSNLVSDDVWQLSLFGDRERKRSLGYVMDDIK--NRFGPTAIL 386


                  ..
gi 2520332465 389 FA 390
Cdd:PRK03103  387 RA 388
IMS pfam00817
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
 5-149 1.32e-38

impB/mucB/samB family; These proteins are involved in UV protection (Swiss).


Pssm-ID: 425885 [Multi-domain]  Cd Length: 148  Bit Score: 136.17  E-value: 1.32e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   5 VDVNNFYTSCETVFRPDLKGKPIVVVSNN-DGCIISRSAEAKSLGIKNGEPFFKI-KNNPyysSVHFFSSNYALYADLSD 82
Cdd:pfam00817   1 IDMDAFFASVELLRDPELKGKPVAVGGGNgRGIVAAASYEARKYGVRSGMPVFEAkKLCP---NLIVVPPDLELYRRASR 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2520332465  83 RVMQTLTAM-SPSIEIYSIDEAFINLSGVMNCVPL-STFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLA 149
Cdd:pfam00817  78 KIFEILRRFsTPKVEQASIDEAFLDLTGLEKLFGAeEALAKRLRREIAEETGLTCSIGIAPNKLLAKLA 146
PRK01810 PRK01810
DNA polymerase IV; Validated
 5-381 7.00e-35

DNA polymerase IV; Validated


Pssm-ID: 179337 [Multi-domain]  Cd Length: 407  Bit Score: 133.23  E-value: 7.00e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   5 VDVNNFYTSCETVFRPDLKGKPIVVVSN---NDGCIISRSAEAKSLGIKNGEPFFKIKNN-PyysSVHFFSSNYALYADL 80
Cdd:PRK01810   10 VDMNSFFASVEIAYDPSLQGKPLAVAGNekeRKGIIVTCSYEARAYGIRTTMPLWEAKRLcP---QLIVRRPNFDRYREA 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  81 SDRVMQTLTAMSPSIEIYSIDEAFINLSGVMNCV-PLSTfGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKTWRGT 159
Cdd:PRK01810   87 SRQMFQILSEFTPLVQPVSIDEGYLDITDCYALGsPLEI-AKMIQQRLLTELQLPCSIGIAPNKFLAKMASDMKKPLGIT 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 160 ggvvdlsnIVRQR---RLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFNVVLERTVRELRGEpcfEL 236
Cdd:PRK01810  166 --------VLRKRdvpEMLWPLPVGEMHGIGEKTAEKLKDIGIQTIGDLAKADEHILRAKLGINGVRLQRRANGI---DD 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 237 QEFSPtkQQIICSRSFGNRVTQYSDM------HQAICAYAERAAEKLRSEHQFCRFVSVFVRtsphavnetYYGNQA--- 307
Cdd:PRK01810  235 RPVDP--EAIYQFKSVGNSTTLSHDMdeekelLDVLRRLSKSVSKRLQKKTVVSYNVQIMIR---------YHDRRTitr 303

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2520332465 308 SMKLLTPSNDTRDIINCATNCLQRIWiDGYRYMKAGVMLADFF--SNGIAQLNLFDDNAPRKNsAALMDVIDQLNQ 381
Cdd:PRK01810  304 SKTLKNPIWEKRDIFQAASRLFKQHW-NGDPVRLLGVTATDLEwkTEAVKQLDLFSFEEDAKE-EPLLAVIDQIND 377
PRK03352 PRK03352
DNA polymerase IV; Validated
 5-335 1.10e-28

DNA polymerase IV; Validated


Pssm-ID: 179564 [Multi-domain]  Cd Length: 346  Bit Score: 115.12  E-value: 1.10e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   5 VDVNNFYTSCETVFRPDLKGKPIVVVSNND-----GCIISRSAEAKSLGIKNGEPF-FKIKNNPyysSVHFFSSNYALYA 78
Cdd:PRK03352   10 VDLDQFIAAVELLRRPELAGLPVIVGGNGDpteprKVVTCASYEARAFGVRAGMPLrTAARRCP---DAVFLPSDPAAYD 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  79 DLSDRVMQTLTAMSPSIEIYSIDEAFInlsGVMNCVPLStFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKTwrg 158
Cdd:PRK03352   87 AASEEVMATLRDLGVPVEVWGWDEAFL---GVDTDDPEA-LAEEIRAAVLERTGLSCSVGIGDNKLRAKIATGFAKP--- 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 159 tGGVVDLSnivrQRRLLALV---PVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFN-------VVLERTV--R 226
Cdd:PRK03352  160 -AGVFRLT----DANWMAVMgdrPTDALWGVGPKTAKRLAALGITTVADLAAADPAELAATFGpttgpwlLLLARGGgdT 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 227 ELRGEPcfelqefsptkqQIICSRS----FGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPhavnetY 302
Cdd:PRK03352  235 EVSAEP------------WVPRSRSrevtFPQDLTDRAEVESAVRELARRVLDEVVAEGRPVTRVAVKVRTAT------F 296

                         330       340       350
                  ....*....|....*....|....*....|...
gi 2520332465 303 YGNQASMKLLTPSNDTRDIINCATNCLQRIWID 335
Cdd:PRK03352  297 YTRTKIRKLPEPTTDPDVIEAAALDVLDRFELD 329
PRK14133 PRK14133
DNA polymerase IV; Provisional
 5-360 2.19e-28

DNA polymerase IV; Provisional


Pssm-ID: 184529 [Multi-domain]  Cd Length: 347  Bit Score: 114.43  E-value: 2.19e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   5 VDVNNFYTSCETVFRPDLKGKPIVVV-SNNDGCIISRSAEAKSLGIKNGEPFFKIKNN-PYYSsvhFFSSNYALYADLSD 82
Cdd:PRK14133    8 VDMDAFFASVEQMDNPKLKGKPVIVGgISERGVVSTCSYEARKYGVHSAMPVFMAKKRcPHGI---FLPVRHERYKEVSK 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  83 RVMQTLTAMSPSIEIYSIDEAFINLSGVmNCVPLSTfGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHaaktWRGTGGV 162
Cdd:PRK14133   85 NIFKILYEVTPIVEPVSIDEAYLDITNI-KEEPIKI-AKYIKKKVKKETGLTLSVGISYNKFLAKLASD----WNKPDGI 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 163 vdlsNIVRQR---RLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHFNVVLERTVRELRGepcFELQEF 239
Cdd:PRK14133  159 ----KIITEDmipDILKPLPISKVHGIGKKSVEKLNNIGIYTIEDLLKLSREFLIEYFGKFGVEIYERIRG---IDYREV 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 240 SPTKQQiicsRSFGNRVTQYSD------MHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPHAVnetyygNQASMKLLT 313
Cdd:PRK14133  232 EVSRER----KSIGKETTLKKDtkdkeeLKKYLKDFSNIISEELKKRNLYGKTVTVKIKTSDFQT------HTKSKTLND 301

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*..
gi 2520332465 314 PSNDTRDIINCATNCLQRIWIDGYRYMkAGVMLADFFSNGIAQLNLF 360
Cdd:PRK14133  302 YIRDKEEIYNVACEILEHINIKEPIRL-IGLSVSNLSENKIEQLSFL 347
PRK03348 PRK03348
DNA polymerase IV; Provisional
 5-294 8.84e-28

DNA polymerase IV; Provisional


Pssm-ID: 235118 [Multi-domain]  Cd Length: 454  Bit Score: 114.26  E-value: 8.84e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   5 VDVNNFYTSCETVFRPDLKGKPIVV--VSNNdGCIISRSAEAKSLGIKNGEPFFKIKNNPYYSSVhFFSSNYALYADLSD 82
Cdd:PRK03348   10 LDMDAFFASVEQLTRPTLRGRPVLVggLGGR-GVVAGASYEARVFGARSAMPMHQARRLVGNGAV-VLPPRFVVYRAASR 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  83 RVMQTLTAMSPSIEIYSIDEAFINLSGVMNCVP--LSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKtwrgTG 160
Cdd:PRK03348   88 RVFDTLRELSPVVEQLSFDEAFVEPAELAGASAeeVEAFAERLRARVREETGLPASVGAGSGKQIAKIASGLAK----PD 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 161 GVVdlsnIVR---QRRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSLwvikKHFNVVLERTV-REL----RGEP 232
Cdd:PRK03348  164 GIR----VVPpgeERELLAPLPVRRLWGIGPVTEEKLHRLGIETIGDLAALSE----AEVANLLGATVgPALhrlaRGID 235

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2520332465 233 CFELQEFSPTKqQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTS 294
Cdd:PRK03348  236 DRPVAERAEAK-QISAESTFAVDLTTRAQLREAIERIAEHAHRRLLKDGRGARTVTVKLRKS 296
PRK01216 PRK01216
DNA polymerase IV; Validated
 1-257 1.30e-26

DNA polymerase IV; Validated


Pssm-ID: 179251 [Multi-domain]  Cd Length: 351  Bit Score: 109.49  E-value: 1.30e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   1 MFALVDVNNFYTSCETVFRPDLKGKPIVVV-----SNNDGCIISRSAEAKSLGIKNGEPFFK---IKNNPYYSSVHffss 72
Cdd:PRK01216    2 IILFVDFDYFFAQVEEVLNPSLKGKPVVVCvysgrFEDSGAVATANYEARKLGIKAGMPIVEakkILPNAVYLPMR---- 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  73 nYALYADLSDRVMQTLTAMSPSIEIYSIDEAFINLSG-VMNCVPLSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANH 151
Cdd:PRK01216   78 -KEVYQQVSNRIMKLLREYSEKIEIASIDEAYLDISDkVKNYQDAYNLGLEIKNKILEKEKITVTVGISKNKVFAKIAAD 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 152 AAKTwrgTG-GVVDLSNIvrqRRLLALVPVEEVWGVGRKMSKKLNLMGINTALDlaesslwVIKKHFNvVLERTVRELRG 230
Cdd:PRK01216  157 MAKP---NGiKVIDDEEV---KRFINELDIADIPGIGDITAEKLKKLGVNKLVD-------TLRIEFD-ELKGIIGEAKA 222

                         250       260       270
                  ....*....|....*....|....*....|
gi 2520332465 231 EPCFELQE---FSPTKQQIIcsRSFGNRVT 257
Cdd:PRK01216  223 KYLFSLARneyNEPVRARVR--KSIGRYVT 250
IMS_C pfam11799
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
 243-353 3.51e-24

impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).


Pssm-ID: 463354 [Multi-domain]  Cd Length: 104  Bit Score: 96.09  E-value: 3.51e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 243 KQQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSPHAvnetyyGNQASMKLLTPSNDTRDII 322
Cdd:pfam11799   1 RKSIGAERTFGRDLTDLEELREALLELAEELAERLRRQGLVARTVTVKIRYSDFR------TITRSVTLPSPTDDTDEIY 74

                          90       100       110
                  ....*....|....*....|....*....|.
gi 2520332465 323 NCATNCLQRIWiDGYRYMKAGVMLADFFSNG 353
Cdd:pfam11799  75 RAALRLLRRLY-RGRPVRLLGVSLSNLVPEG 104
PRK03858 PRK03858
DNA polymerase IV; Validated
 6-292 2.07e-22

DNA polymerase IV; Validated


Pssm-ID: 179663 [Multi-domain]  Cd Length: 396  Bit Score: 98.13  E-value: 2.07e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   6 DVNNFYTSCETVFRPDLKGKPIVVvsnNDGCIISRSAEAKSLGIKNGEPFFKIKN-NPYYSSVhffSSNYALYADLSDRV 84
Cdd:PRK03858   10 DLDSFYASVEQRDDPALRGRPVIV---GGGVVLAASYEAKAYGVRTAMGGRQARRlCPQAVVV---PPRMSAYSRASKAV 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  85 MQTLTAMSPSIEIYSIDEAFINLSGVMNCV-PLSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKtwrgTGGVV 163
Cdd:PRK03858   84 FEVFRDTTPLVEGLSIDEAFLDVGGLRRISgTPVQIAARLRRRVREEVGLPITVGVARTKFLAKVASQVAK----PDGLL 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 164 dlsnIV---RQRRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLAESSlwvikkhfNVVLERTVRELRGEPCFELQ--- 237
Cdd:PRK03858  160 ----VVppdRELAFLHPLPVRRLWGVGPVTAAKLRAHGITTVGDVAELP--------ESALVSLLGPAAGRHLHALAhnr 227

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 238 -----EFSPTKQQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLRSEHQFCRFVSVFVR 292
Cdd:PRK03858  228 dprrvETGRRRRSVGAQRALGRGPNSPAEVDAVVVALVDRVARRMRAAGRTGRTVVLRLR 287
DUF4113 pfam13438
Domain of unknown function (DUF4113); Although the function is not known this domain occurs ...
 372-419 1.12e-20

Domain of unknown function (DUF4113); Although the function is not known this domain occurs almost invariably at the very C-terminus of the IMS family DNA-polymerase repair proteins, IMS, pfam00817.


Pssm-ID: 463876 [Multi-domain]  Cd Length: 49  Bit Score: 84.46  E-value: 1.12e-20
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 2520332465 372 LMDVIDQLNQS-GKGKIWFAGQGIEKSWAMKREMLSPAYTTRYSDLPVA 419
Cdd:pfam13438   1 LMAALDAINRRyGRGTVRLAAQGLKRDWKMRREHLSPRYTTRWDELPVV 49
PolY_Rev1 cd01701
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ...
 5-278 3.33e-20

DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.


Pssm-ID: 176455 [Multi-domain]  Cd Length: 404  Bit Score: 91.61  E-value: 3.33e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   5 VDVNNFYTSCETVFRPDLKGKPiVVVSNNDGC---IISRSAEAKSLGIKNGEPFFKIKN-------NPY----YSSVHFf 70
Cdd:cd01701    52 VDFDCFFVSVSIRNRPDLKGKP-VAVCHGKGPnseIASCNYEARSYGIKNGMWVGQAKKlcpqlvtLPYdfeaYEEVSL- 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  71 ssnyALYadlsdrvmQTLTAMSPSIEIYSIDEAFINLSGVMNCVPLST--FGHEIRTNVLKNTGLTVGVGIAQTKTLAKL 148
Cdd:cd01701   130 ----TFY--------EILASYTDNIEAVSCDEALIDITSLLEETYELPeeLAEAIRNEIRETTGCSASVGIGPNILLARL 197

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 149 ANHAAKtwrgTGGV-VDLSNIVRQrrLLALVPVEEVWGVGRKMSKKLNLMGINTA--LDLAESSLWVIKKHFNVVL-ERT 224
Cdd:cd01701   198 ATRKAK----PDGQyHLSAEKVEE--FLSQLKVGDLPGVGSSLAEKLVKLFGDTCggLELRSKTKEKLQKVLGPKTgEKL 271

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....
gi 2520332465 225 VRELRGEPCFELqEFSPTKQQIICSRSFGNRVTQYSDMHQAICAYAERAAEKLR 278
Cdd:cd01701   272 YDYCRGIDDRPV-TGEKERKSVSAEINYGIRFTNVDDVEQFLQRLSEELSKRLE 324
PolY_Pol_eta cd01702
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ...
 3-257 5.51e-18

DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.


Pssm-ID: 176456 [Multi-domain]  Cd Length: 359  Bit Score: 84.67  E-value: 5.51e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   3 ALVDVNNFYTSCETVFRPDLKGKPIVVVSNNDgcIISRSAEAKSLGIKNGE-----------------PFFKIKNNPYYS 65
Cdd:cd01702     1 AHIDMDAFFAQVEQVRLGLLRNDPVAVVQWNS--IIAVSYAARAFGVTRFMtideakkkcpdlilahvATYKKGEDEADY 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  66 SVHFFSSNY--AL--YADLSDRVMQTLTAMSPSIEIYSIDEAFINL-SGVMncvplstfgHEIRTNVLKNTGLTVGVGIA 140
Cdd:cd01702    79 HENPSPARHkvSLdpYRRASRKILNILKRFGDVVEKASIDEAYLDLgSRIV---------EEIRQQVYDELGYTCSAGIA 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 141 QTKTLAKLANHAAKTWRGTggvvdlsnIVRQRRLLALV---PVEEVWGVGRKMSKKL-NLMGINTALDLA--ESSLWVIK 214
Cdd:cd01702   150 HNKMLAKLASGMNKPNAQT--------ILRNDAVASFLsslPITSIRGLGGKLGEEIiDLLGLPTEGDVAgfRSSESDLQ 221

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....
gi 2520332465 215 KHFNVVLERTV-RELRGEPCFELQEfSPTKQQIICSRSFGNRVT 257
Cdd:cd01702   222 EHFGEKLGEWLyNLLRGIDHEPVKP-RPLPKSMGSSKNFPGKTA 264
PRK02406 PRK02406
DNA polymerase IV; Validated
 10-217 1.03e-15

DNA polymerase IV; Validated


Pssm-ID: 235035 [Multi-domain]  Cd Length: 343  Bit Score: 77.85  E-value: 1.03e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  10 FYTSCETVFRPDLKGKPIVVVSNND--GCIISRSAEAKSLGIKNGEPffkiknnpyysSVH---------FFSSNYALYA 78
Cdd:PRK02406    4 FYAAVEMRDNPELRGKPVAVGGSPGrrGVISTCNYEARKFGVRSAMP-----------TAQalklcpdliFVPGRFDVYK 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  79 DLSDRVMQTLTAMSPSIEIYSIDEAFINLSGVMNCVPLST-FGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHaaktWR 157
Cdd:PRK02406   73 EVSRQIREIFRRYTDLIEPLSLDEAYLDVTDNKLCIGSATlIAQEIRQDIFEELGLTASAGVAPNKFLAKIASD----WN 148

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2520332465 158 GTGG--VVDLSNIvrQRRLLALvPVEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKKHF 217
Cdd:PRK02406  149 KPNGlfVITPEEV--DAFLATL-PVEKIPGVGKVTAEKLHALGIYTCADLQKYDLAELIRHF 207
PRK02794 PRK02794
DNA polymerase IV; Provisional
 3-206 1.09e-15

DNA polymerase IV; Provisional


Pssm-ID: 179473 [Multi-domain]  Cd Length: 419  Bit Score: 78.44  E-value: 1.09e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   3 ALVDVNNFYTSCETVFRPDLKGKPIV-------VVSNndGCIISRSAeakslGIKNGEPFFK----------IKnnPyys 65
Cdd:PRK02794   39 AHIDCDAFYASVEKRDNPELRDKPVIigggkrgVVST--ACYIARIH-----GVRSAMPMFKalklcpdavvIK--P--- 106

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  66 svhffssNYALYADLSDRVMQTLTAMSPSIEIYSIDEAFINLSG----------VMncvpLSTFGHEIRTNVlkntGLTV 135
Cdd:PRK02794  107 -------DMEKYVRVGREVRAMMQALTPLVEPLSIDEAFLDLSGterlhgappaVV----LARFARRVEREI----GITV 171

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2520332465 136 GVGIAQTKTLAKLANHAAKTwRGtggvvdLSNIVRQ--RRLLALVPVEEVWGVGRKMSKKLNLMGINTALDLA 206
Cdd:PRK02794  172 SVGLSYNKFLAKIASDLDKP-RG------FSVIGRAeaLAFLAPKPVGIIWGVGPATAARLARDGIRTIGDLQ 237
PolY_Pol_iota cd01703
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ...
 4-209 1.27e-07

DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.


Pssm-ID: 176457 [Multi-domain]  Cd Length: 379  Bit Score: 53.24  E-value: 1.27e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   4 LVDVNNFYTSCETVFRPDLKGKPIVVVSNNdgCIISRSAEAKSLGIK------------------NGEpffkiknnpyys 65
Cdd:cd01703     2 HLDLDCFYAQVEEIRDPSLKSKPLGIQQKY--IVVTCNYEARRLGVKklmsikdakeicpdlvlvNGE------------ 67

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  66 svhffssNYALYADLSDRVMQTLTAMSPS--IEIYSIDEAFINLSGvMNCVPLSTFGHEIRTNVLKNTGLTVGVGIAQTK 143
Cdd:cd01703    68 -------DLTPFRDMSKKVYRLLRSYSWNdrVERLGFDENFMDVTE-MRLLVASHIAYEMRERIENELGLTCCAGIASNK 139

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 144 TLAKLANHAAKTWRGTG----GVVDLSNIVRQRRLLAlVPveevwGVGRKMSKKLNLMGINTALDLAESS 209
Cdd:cd01703   140 LLAKLVGSVNKPNQQTTllppSCADLMDFMDLHDLRK-IP-----GIGYKTAAKLEAHGISSVRDLQEFS 203
PTZ00205 PTZ00205
DNA polymerase kappa; Provisional
 5-318 1.53e-07

DNA polymerase kappa; Provisional


Pssm-ID: 140232 [Multi-domain]  Cd Length: 571  Bit Score: 53.48  E-value: 1.53e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465   5 VDVNNFYTSCETVFRPDLKGKPIVVVSNNdgCIISRSAEAKSLGIKNGEP-FFKIKNNPyysSVHFFSSNYALYADLSDR 83
Cdd:PTZ00205  138 LDMDMFYAAVEIKKHPEYAAIPLAIGTMT--MLQTANYVARGRGIRQGMPgFLALKICP---NLLILPPDFDAYNEESNT 212

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465  84 VMQTLTAMSPSIEIYSIDEAFINLSGVMN----CVPLSTFGHEIRTNVLKNTGLTVGVGIAQTKTLAKLANHAAKtwrgT 159
Cdd:PTZ00205  213 VRRIVAEYDPNYISFGLDELTLEVSAYIErfegTKTAEDVASELRVRVFGETKLTASAGIGPTAALAKIASNINK----P 288

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 160 GGVVDLS-----NIVRQRRLLALvpvEEVWGVGRKMSKKLNLMGINTALDLAESSLWVIKkhfnVVLERTVRELRGEPCF 234
Cdd:PTZ00205  289 NGQHDLNlhtrgDVMTYVRDLGL---RSVPGVGKVTEALLKGLGITTLSDIYNRRVELCY----ILHNNLFRFLLGASIG 361

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2520332465 235 ELQ---------------EFSPTKQQIICSRSFGNRVTQySDMHQAICAYAERAAEKLRSEHQFCRFVSVFVRTSphavn 299
Cdd:PTZ00205  362 IMQwpdaataantencegATGGQRKAISSERSFTTPRTK-EGLQEMVDTVFNGAYEEMRKSELMCRQISLTIRWA----- 435

                         330
                  ....*....|....*....
gi 2520332465 300 eTYYGNQASMKLLTPSNDT 318
Cdd:PTZ00205  436 -SYRYQQYTKSLIQYSDDS 453
IMS_HHH pfam11798
IMS family HHH motif; These proteins are involved in UV protection, eg.
 172-199 2.45e-04

IMS family HHH motif; These proteins are involved in UV protection, eg.


Pssm-ID: 432081 [Multi-domain]  Cd Length: 32  Bit Score: 38.15  E-value: 2.45e-04
                          10        20
                  ....*....|....*....|....*...
gi 2520332465 172 RRLLALVPVEEVWGVGRKMSKKLNLMGI 199
Cdd:pfam11798   5 PEFLWPLPISKIPGIGKKLAEKLKALGI 32
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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