nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2221266685   6 AIEDRHSVRVFTNKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLVRGKSLAKLKEL 61
Cdd:cd02136     1 AIKSRRSVRAFKDKPVPKETIEKILEAARRAPSGKNTQPWRVYVVTGKARERLKKA 56

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2221266685   6 AIEDRHSVRVFTNKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLVRGKSLAKLKEL 61
Cdd:cd02136     1 AIKSRRSVRAFKDKPVPKETIEKILEAARRAPSGKNTQPWRVYVVTGKARERLKKA 56

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2221266685   7 IEDRHSVRVFTNKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLV-RGKSLAKLKElHLKNSKTDRSAAPE-WPTMHRVNW 84
Cdd:pfam00881   1 IRQRRSVRKFDPEPVPKEVLEEILEAARRAPSAGNLQPWRFYVVtDGELRYRLAE-AALELLLVEPAAALlLLLRRDANL 79

                  ....*....
gi 2221266685  85 DEFPRDNMA 93
Cdd:pfam00881  80 KLLLQDFLR 88

oxygen-insensitive NAD(P)H nitroreductase;

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 2221266685   1 METKQAIEDRHSVRVF-TNKDVEVNKLKGLVKLAQKAPSWVDSQPW 45
Cdd:PRK11053    1 MDIVSVAKKRYTTKAFdPSKKLPAEQIEQIKTLLRFSPSSVNSQPW 46

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2221266685   6 AIEDRHSVRVFTNKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLVRGKSLAKLKEL 61
Cdd:cd02136     1 AIKSRRSVRAFKDKPVPKETIEKILEAARRAPSGKNTQPWRVYVVTGKARERLKKA 56

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2221266685   7 IEDRHSVRVFTNKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLVRGKslAKLKELHLK 64
Cdd:cd02062     1 IKTRRSIRKFTDKPVPEEKLRKILEAARLAPSAGNLQPWRFIVVRDR--EKKEKLAKL 56

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 2221266685   4 KQAIEDRHSVRVFTNKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLVRGK-SLAKLKEL 61
Cdd:cd20609     3 LELAKKRYSVRKFSDKPVEKEKLDKILEAGRLAPTAVNYQPQRILVVRSEeALEKLAKA 61

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2221266685   5 QAIEDRHSVRVFTNKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLVRGKslaKLKE 60
Cdd:cd02139     3 EAIKKRRSIRKYKPTPVEEEKLLRILEAARLAPSAKNRQPWRFIVVKDK---ELKE 55

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2221266685   4 KQAIEDRHSVRVFT-NKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLVRGK-SLAKLKELHLKNSKTDRSAA 73
Cdd:cd02137     1 LEVIKSRRSVRNFDpDHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPeLKAKLAEAAYNQPQVTTASA 72

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2221266685   7 IEDRHSVRVFTNKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLVRGKslAKLKELH 62
Cdd:cd20610     1 IKKRRSIRKFKPDPVPKEDIEKILEAANWAPSGMNRQNWEFVVVKGG--EKIEKIG 54

nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to Burkholderia pseudomallei TdsD, may be involved in the processing of organosulfur compounds. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 2221266685   6 AIEDRHSVRVFTNKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLVR 51
Cdd:cd02138     1 LIAERWSPRAFSPEPISEEDLLSLFEAARWAPSCFNEQPWRFVVAR 46

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2221266685   4 KQAIEDRHSVRVF-TNKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLVRGKSL-AKLKELHLKN 65
Cdd:cd02149     3 LELLNFRYATKKFdPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELkAKLAPAAWFN 66

5,6-dimethylbenzimidazole synthase; BluB catalyzes the O2-dependent conversion of FMNH2 to 5,6-dimethylbenzimidazole (DMB), a component of vitamin B12; is is a subfamily of the nitroreductase family; nitroreductases typically reduce their substrates by using NAD(P)H as electron donor and often use FMN as a cofactor.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2221266685   5 QAIEDRHSVRVFTNKDVEVNKLKGLVKLAQKAPSWVDSQPWKIYLVRgkSLAKLKELH 62
Cdd:cd02145     2 RVIRWRRDVRHFRPDPVPEEVLERLLQAAHLAPSVGLMQPWRFVRVR--SAATRKAVH 57

nitroreductases with an N-terminal iron-sulfur cluster-binding domain; Members of this family utilize FMN as a cofactor. This family may be involved in the reduction of flavin or nitroaromatic compounds via an obligatory two-electron transfer. Nitroreductase is homodimer. Each subunit contains one FMN molecule.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 2221266685  10 RHSVRVFTNKDVEVNKLKGLVKLAQKAPSWVDSQPWKiYLV 50
Cdd:cd02143     5 RRSIRRYKDKPVPRETLEKLLDIARYAPTGHNSQPVH-WLV 44

oxygen-insensitive NAD(P)H nitroreductase;

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 2221266685   1 METKQAIEDRHSVRVF-TNKDVEVNKLKGLVKLAQKAPSWVDSQPW 45
Cdd:PRK11053    1 MDIVSVAKKRYTTKAFdPSKKLPAEQIEQIKTLLRFSPSSVNSQPW 46