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Conserved domains on  [gi|1893881370|ref|WP_185746602|]
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MULTISPECIES: poly-gamma-glutamate hydrolase family protein [Mycolicibacter]

Protein Classification

gamma-glutamylcyclotransferase family protein( domain architecture ID 10159951)

gamma-glutamylcyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Gamma_PGA_hydro super family cl01713
Poly-gamma-glutamate hydrolase; This family consists of a number of bacterial and phage ...
 158-340 1.43e-39

Poly-gamma-glutamate hydrolase; This family consists of a number of bacterial and phage proteins that function as gamma-PGA hydrolase enzymes. Structurally the protein in this family adopted an open alpha/beta mixed core structure with a seven-stranded parallel/anti-parallel beta-sheet. This structure shows similarity to mammalian carboxypeptidase A and related enzymes.


The actual alignment was detected with superfamily member pfam05908:

Pssm-ID: 470314  Cd Length: 191  Bit Score: 138.54  E-value: 1.43e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370 158 QSLSELLA--DPGV---IEESHLRSRFGFLAVHGGGLEQMTDVIaDRAAEAADASVYLVRHPD---GYPRHLPSARYLAA 229
Cdd:pfam05908   2 SSFEELKEneTEGKdyrIRERERGSRLLVLAPHGGGIEPGTSEI-AEAIAGADYSFYLFEGLKpsgNRELHITSTRFDEP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370 230 ESARLAEfldHVDVVVSLHGYGRIGrsTQLLAGGCNRRLAAHVARCLDLPGYQVitDLNAIPVGLRGLHPANPVNQVR-G 308
Cdd:pfam05908  81 LALQLAK---GHDYVISIHGYGGEE--EHTLVGGTDRDLAEAIVEALEDAGFSA--ELAPEGSRLAGTSPDNICNRCKtG 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 1893881370 309 GGTQLELSPRVR----------GISPRSqppgADGLSPVTSA 340
Cdd:pfam05908 154 EGVQLELSRALRkaffdnfdlrGRSRTQ----TEVFYRYVSA 191
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 8-103 1.94e-25

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


:

Pssm-ID: 119400  Cd Length: 99  Bit Score: 98.19  E-value: 1.94e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370   8 YFAYGSNLCAQQMARRCPEACDPRPAVLADHDWLINQR-GVATVEPFPGSRVHGVLWRVSDRDLAALDSAEGVPVRYRRD 86
Cdd:cd06661     1 LFVYGTLMDGEVLHARLGRALFLGPATLKGYRLVFGGGsGYPGLVPGPGARVWGELYEVDPEDLARLDAFEGVPGGYRRE 80

                          90
                  ....*....|....*...
gi 1893881370  87 RLTVHTTDG-SAPAWVYI 103
Cdd:cd06661    81 EVEVELEDGeGVEAWVYV 98
 
Name Accession Description Interval E-value
Gamma_PGA_hydro pfam05908
Poly-gamma-glutamate hydrolase; This family consists of a number of bacterial and phage ...
 158-340 1.43e-39

Poly-gamma-glutamate hydrolase; This family consists of a number of bacterial and phage proteins that function as gamma-PGA hydrolase enzymes. Structurally the protein in this family adopted an open alpha/beta mixed core structure with a seven-stranded parallel/anti-parallel beta-sheet. This structure shows similarity to mammalian carboxypeptidase A and related enzymes.


Pssm-ID: 399122  Cd Length: 191  Bit Score: 138.54  E-value: 1.43e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370 158 QSLSELLA--DPGV---IEESHLRSRFGFLAVHGGGLEQMTDVIaDRAAEAADASVYLVRHPD---GYPRHLPSARYLAA 229
Cdd:pfam05908   2 SSFEELKEneTEGKdyrIRERERGSRLLVLAPHGGGIEPGTSEI-AEAIAGADYSFYLFEGLKpsgNRELHITSTRFDEP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370 230 ESARLAEfldHVDVVVSLHGYGRIGrsTQLLAGGCNRRLAAHVARCLDLPGYQVitDLNAIPVGLRGLHPANPVNQVR-G 308
Cdd:pfam05908  81 LALQLAK---GHDYVISIHGYGGEE--EHTLVGGTDRDLAEAIVEALEDAGFSA--ELAPEGSRLAGTSPDNICNRCKtG 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 1893881370 309 GGTQLELSPRVR----------GISPRSqppgADGLSPVTSA 340
Cdd:pfam05908 154 EGVQLELSRALRkaffdnfdlrGRSRTQ----TEVFYRYVSA 191
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 8-103 1.94e-25

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 98.19  E-value: 1.94e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370   8 YFAYGSNLCAQQMARRCPEACDPRPAVLADHDWLINQR-GVATVEPFPGSRVHGVLWRVSDRDLAALDSAEGVPVRYRRD 86
Cdd:cd06661     1 LFVYGTLMDGEVLHARLGRALFLGPATLKGYRLVFGGGsGYPGLVPGPGARVWGELYEVDPEDLARLDAFEGVPGGYRRE 80

                          90
                  ....*....|....*...
gi 1893881370  87 RLTVHTTDG-SAPAWVYI 103
Cdd:cd06661    81 EVEVELEDGeGVEAWVYV 98
YtfP COG2105
Predicted gamma-glutamylamine cyclotransferase YtfP, GGCT/AIG2-like family [Amino acid ...
 8-113 1.08e-18

Predicted gamma-glutamylamine cyclotransferase YtfP, GGCT/AIG2-like family [Amino acid transport and metabolism];


Pssm-ID: 441708 [Multi-domain]  Cd Length: 114  Bit Score: 80.70  E-value: 1.08e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370   8 YFAYGSNLCAQQMARRCPEACDPRPAVLADHdwLINQRGVATVEPFPGSRVHGVLWRVSDRDLAALDSAEGVPVRYRRDR 87
Cdd:COG2105     3 LFVYGTLRRGESNHHLLRGARFLGPATLEGR--LYDLGAYPALVPDGGGRVYGELYEVDDALLAALDELEGAPNLYRREL 80

                          90       100
                  ....*....|....*....|....*.
gi 1893881370  88 LTVHTTDGSAPAWVYIDHRVQPGAPR 113
Cdd:COG2105    81 VPVETGDGTVEAWVYVYNPEVSGLPR 106
GGACT pfam06094
Gamma-glutamyl cyclotransferase, AIG2-like; GGACT, gamma-glutamylamine cyclotransferase, is a ...
 8-103 2.63e-15

Gamma-glutamyl cyclotransferase, AIG2-like; GGACT, gamma-glutamylamine cyclotransferase, is a ubiquitous enzyme found in bacteria, plants, and metazoans from Dictyostelium through to humans. It converts gamma-glutamylamines to free amines and 5-oxoproline.


Pssm-ID: 428763 [Multi-domain]  Cd Length: 118  Bit Score: 71.27  E-value: 2.63e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370   8 YFAYGSNLCAQ--QMARRC-PEACDPRPAVLADHDwLINQRGVATVEPFPGSRVHGVLWRVSDRDLAALDSAEGVPVRYR 84
Cdd:pfam06094   1 VFVYGTLRRGEpnHHALLLgARFPGLGPATLPGYR-LYDLGGYPALVPGEGERVVGELYEVDDALLARLDELEGYPNLYE 79

                          90       100
                  ....*....|....*....|
gi 1893881370  85 RDRLTVHTTDGSA-PAWVYI 103
Cdd:pfam06094  80 REVVEVEVEDGEPvEAWVYV 99
 
Name Accession Description Interval E-value
Gamma_PGA_hydro pfam05908
Poly-gamma-glutamate hydrolase; This family consists of a number of bacterial and phage ...
 158-340 1.43e-39

Poly-gamma-glutamate hydrolase; This family consists of a number of bacterial and phage proteins that function as gamma-PGA hydrolase enzymes. Structurally the protein in this family adopted an open alpha/beta mixed core structure with a seven-stranded parallel/anti-parallel beta-sheet. This structure shows similarity to mammalian carboxypeptidase A and related enzymes.


Pssm-ID: 399122  Cd Length: 191  Bit Score: 138.54  E-value: 1.43e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370 158 QSLSELLA--DPGV---IEESHLRSRFGFLAVHGGGLEQMTDVIaDRAAEAADASVYLVRHPD---GYPRHLPSARYLAA 229
Cdd:pfam05908   2 SSFEELKEneTEGKdyrIRERERGSRLLVLAPHGGGIEPGTSEI-AEAIAGADYSFYLFEGLKpsgNRELHITSTRFDEP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370 230 ESARLAEfldHVDVVVSLHGYGRIGrsTQLLAGGCNRRLAAHVARCLDLPGYQVitDLNAIPVGLRGLHPANPVNQVR-G 308
Cdd:pfam05908  81 LALQLAK---GHDYVISIHGYGGEE--EHTLVGGTDRDLAEAIVEALEDAGFSA--ELAPEGSRLAGTSPDNICNRCKtG 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 1893881370 309 GGTQLELSPRVR----------GISPRSqppgADGLSPVTSA 340
Cdd:pfam05908 154 EGVQLELSRALRkaffdnfdlrGRSRTQ----TEVFYRYVSA 191
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 8-103 1.94e-25

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 98.19  E-value: 1.94e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370   8 YFAYGSNLCAQQMARRCPEACDPRPAVLADHDWLINQR-GVATVEPFPGSRVHGVLWRVSDRDLAALDSAEGVPVRYRRD 86
Cdd:cd06661     1 LFVYGTLMDGEVLHARLGRALFLGPATLKGYRLVFGGGsGYPGLVPGPGARVWGELYEVDPEDLARLDAFEGVPGGYRRE 80

                          90
                  ....*....|....*...
gi 1893881370  87 RLTVHTTDG-SAPAWVYI 103
Cdd:cd06661    81 EVEVELEDGeGVEAWVYV 98
YtfP COG2105
Predicted gamma-glutamylamine cyclotransferase YtfP, GGCT/AIG2-like family [Amino acid ...
 8-113 1.08e-18

Predicted gamma-glutamylamine cyclotransferase YtfP, GGCT/AIG2-like family [Amino acid transport and metabolism];


Pssm-ID: 441708 [Multi-domain]  Cd Length: 114  Bit Score: 80.70  E-value: 1.08e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370   8 YFAYGSNLCAQQMARRCPEACDPRPAVLADHdwLINQRGVATVEPFPGSRVHGVLWRVSDRDLAALDSAEGVPVRYRRDR 87
Cdd:COG2105     3 LFVYGTLRRGESNHHLLRGARFLGPATLEGR--LYDLGAYPALVPDGGGRVYGELYEVDDALLAALDELEGAPNLYRREL 80

                          90       100
                  ....*....|....*....|....*.
gi 1893881370  88 LTVHTTDGSAPAWVYIDHRVQPGAPR 113
Cdd:COG2105    81 VPVETGDGTVEAWVYVYNPEVSGLPR 106
GGACT pfam06094
Gamma-glutamyl cyclotransferase, AIG2-like; GGACT, gamma-glutamylamine cyclotransferase, is a ...
 8-103 2.63e-15

Gamma-glutamyl cyclotransferase, AIG2-like; GGACT, gamma-glutamylamine cyclotransferase, is a ubiquitous enzyme found in bacteria, plants, and metazoans from Dictyostelium through to humans. It converts gamma-glutamylamines to free amines and 5-oxoproline.


Pssm-ID: 428763 [Multi-domain]  Cd Length: 118  Bit Score: 71.27  E-value: 2.63e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370   8 YFAYGSNLCAQ--QMARRC-PEACDPRPAVLADHDwLINQRGVATVEPFPGSRVHGVLWRVSDRDLAALDSAEGVPVRYR 84
Cdd:pfam06094   1 VFVYGTLRRGEpnHHALLLgARFPGLGPATLPGYR-LYDLGGYPALVPGEGERVVGELYEVDDALLARLDELEGYPNLYE 79

                          90       100
                  ....*....|....*....|
gi 1893881370  85 RDRLTVHTTDGSA-PAWVYI 103
Cdd:pfam06094  80 REVVEVEVEDGEPvEAWVYV 99
AIG2_2 pfam13772
AIG2-like family; This family is found in bacteria and metazoa.
 57-137 1.19e-12

AIG2-like family; This family is found in bacteria and metazoa.


Pssm-ID: 433465  Cd Length: 83  Bit Score: 62.66  E-value: 1.19e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1893881370  57 RVHGVLWRVSDRDLAALDSAEGVPVR-YRRDRLTVHTTDGSA-PAWVYIDHRVQPGAPRDGYLERIIGAAGQHGLPPAWI 134
Cdd:pfam13772   1 HVFGVLWEIPMENLKDLDKQEGVEDGiYVPITVPVSTATGTEvLARAYVLSNQPEGPPSETYLKVLVRGAEESGLPEDYV 80


                  ...
gi 1893881370 135 EFL 137
Cdd:pfam13772  81 KRL 83
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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