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Conserved domains on  [gi|1359830433|ref|WP_105857090|]
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plasmid recombination protein [Burkholderia vietnamiensis]

Protein Classification

plasmid recombination protein( domain architecture ID 15322803)

plasmid recombination protein belongs to the plasmid mobilization pre family

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MobM_relaxase cd17242
relaxase domain of MobM and similar proteins; With some plasmids, recombination can occur in a ...
 29-220 1.16e-34

relaxase domain of MobM and similar proteins; With some plasmids, recombination can occur in a site-specific manner that is independent of RecA. In such cases, the recombination event requires another protein called Pre (plasmid recombination enzyme), also known as Mob (conjugative mobilization). The best characterized member of this family is encoded by the streptococcal plasmid pMV158 that recognizes the plasmid origin of transfer. MobM converts supercoiled plasmid DNA into relaxed DNA by cleaving a phosphodiester bond of a specific dinucleotide and remains bound to the 5'-end of the nick site.


:

Pssm-ID: 410988  Cd Length: 196  Bit Score: 126.22  E-value: 1.16e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433  29 AAAEHNLRLRTQA--NIDADRSHLNQILYNPLGIDSNDAtsFQRKLSEHYSslGIKIKAENTLAFEYVATASPKFFEGKT 106
Cdd:cd17242    14 GALRHNERERETEnpNIDPSRTHLNYELVGPGKKDYAEA--IKERLEELGK--KKKIRKDAVLAVEFILTASPEFFEGLD 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433 107 TEQIGKWAYHQVEFMRKEF-ADKLQFAVLHLDETTPHIHFFVSteqrsikkyknrygdCEKETWSLNSEKI--NPEYLRT 183
Cdd:cd17242    90 PEEIEEFFKDALEFLKERFgEENIVSAVVHLDETTPHLHAVVV---------------PITEDGRLSAKELfgGRKKLRE 154

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 1359830433 184 LQDNYAKSN-KIFGLSRGAKYSKLKNTTLKDFYRTMKK 220
Cdd:cd17242   155 LQDDYAEAVaKKGGLERGIKGSKAKHKTKEKELKAKHE 192
 
Name Accession Description Interval E-value
MobM_relaxase cd17242
relaxase domain of MobM and similar proteins; With some plasmids, recombination can occur in a ...
 29-220 1.16e-34

relaxase domain of MobM and similar proteins; With some plasmids, recombination can occur in a site-specific manner that is independent of RecA. In such cases, the recombination event requires another protein called Pre (plasmid recombination enzyme), also known as Mob (conjugative mobilization). The best characterized member of this family is encoded by the streptococcal plasmid pMV158 that recognizes the plasmid origin of transfer. MobM converts supercoiled plasmid DNA into relaxed DNA by cleaving a phosphodiester bond of a specific dinucleotide and remains bound to the 5'-end of the nick site.


Pssm-ID: 410988  Cd Length: 196  Bit Score: 126.22  E-value: 1.16e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433  29 AAAEHNLRLRTQA--NIDADRSHLNQILYNPLGIDSNDAtsFQRKLSEHYSslGIKIKAENTLAFEYVATASPKFFEGKT 106
Cdd:cd17242    14 GALRHNERERETEnpNIDPSRTHLNYELVGPGKKDYAEA--IKERLEELGK--KKKIRKDAVLAVEFILTASPEFFEGLD 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433 107 TEQIGKWAYHQVEFMRKEF-ADKLQFAVLHLDETTPHIHFFVSteqrsikkyknrygdCEKETWSLNSEKI--NPEYLRT 183
Cdd:cd17242    90 PEEIEEFFKDALEFLKERFgEENIVSAVVHLDETTPHLHAVVV---------------PITEDGRLSAKELfgGRKKLRE 154

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 1359830433 184 LQDNYAKSN-KIFGLSRGAKYSKLKNTTLKDFYRTMKK 220
Cdd:cd17242   155 LQDDYAEAVaKKGGLERGIKGSKAKHKTKEKELKAKHE 192
MobV NF041497
MobV family relaxase;
29-208 3.06e-22

MobV family relaxase;


Pssm-ID: 469385  Cd Length: 187  Bit Score: 92.63  E-value: 3.06e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433  29 AAAEHNLRL---RTQANIDADRSHLNQILYNPLGIDSNDATSFQRKLSEHYSSLgIKIKAENTLAFEYVATASPKFFEGK 105
Cdd:NF041497   18 GSQEHNEREtenRTNPNADPSRTHLNFELVNGPDGPIDYLEAIKERIAENAGIK-RKIRKDAVRAVEFILTGSPEFFKEL 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433 106 TTE-QIGKWAYHQVEFMRKEF-ADKLQFAVLHLDETTPHIHF-FVSTEQRSIKKYKnrygdceketwslnsEKINPEYLR 182
Cdd:NF041497   97 ADPgELKEWFEDNYDFLADRYgEENIVSAVVHLDETTPHIHAtVVPITEDGRLSAK---------------DVFGRKKLS 161

                         170       180
                  ....*....|....*....|....*.
gi 1359830433 183 TLQDNYAKSNKIFGLSRGAKYSKLKN 208
Cdd:NF041497  162 QLQDDYAEAMKKYGLERGISGSEAKH 187
Mob_Pre pfam01076
Plasmid recombination enzyme; With some plasmids, recombination can occur in a site specific ...
 33-215 5.17e-14

Plasmid recombination enzyme; With some plasmids, recombination can occur in a site specific manner that is independent of RecA. In such cases, the recombination event requires another protein called Pre. Pre is a plasmid recombination enzyme. This protein is also known as Mob (conjugative mobilization). This family is also known as Mob-V. One of the family members, MobM, is encoded by a promiscuous plasmid actively involved in the spread of antibiotic resistance. Homologs of MobM are found in many plasmids and other mobile genetic elements of pathogenic bacteria, including S. aureus. MobM is a metal-dependent nuclease that uses histidine nitrogen for the nucleophilic attack on the scissile phosphate. Furthermore, in contrast to other DNA-processing enzymes, MobM is a histidine relaxase, a DNA-breaking and -joining enzyme, that operates through a phosphorus-nitrogen protein-DNA adduct for cell-to-cell DNA transfer. Mutational analysis indicate that the H(N/D)(Q/E)R N-terminal motif of MobM plays a crucial role in the cleavage and generation of stable DNA-protein adducts.


Pssm-ID: 395854  Cd Length: 195  Bit Score: 70.04  E-value: 5.17e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433  33 HNLRLRTQA---NIDADRSHLNQILYNPLGIDsndatsFQRKLSEHYSSLGI---KIKAENTLAFEYVATASPKFFEGKT 106
Cdd:pfam01076  22 HNQRENETYdnkDIDPERSGLNYDLVNDKQIR------YDEDIKDYIKENQKskrKIRKDAVLENEILITSDPDFFEQLT 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433 107 TEQIGKWAYHQVEFMRKEF-ADKLQFAVLHLDETTPHIHFFVSTeqrsikkyknrygdcEKETWSLNSEKI--NPEYLRT 183
Cdd:pfam01076  96 YEEAKEFFETAFQFFEERYgKENVLYAVVHLDEATPHMHMGVVP---------------GTEDGRLSAKAIfgNRKELIA 160

                         170       180       190
                  ....*....|....*....|....*....|....
gi 1359830433 184 LQDNYAK--SNKIFGLSRGAKYSKLKNTTLKDFY 215
Cdd:pfam01076 161 LQDRFPEymGLKGFDLERGEPGSERKHLGTNEYK 194
 
Name Accession Description Interval E-value
MobM_relaxase cd17242
relaxase domain of MobM and similar proteins; With some plasmids, recombination can occur in a ...
 29-220 1.16e-34

relaxase domain of MobM and similar proteins; With some plasmids, recombination can occur in a site-specific manner that is independent of RecA. In such cases, the recombination event requires another protein called Pre (plasmid recombination enzyme), also known as Mob (conjugative mobilization). The best characterized member of this family is encoded by the streptococcal plasmid pMV158 that recognizes the plasmid origin of transfer. MobM converts supercoiled plasmid DNA into relaxed DNA by cleaving a phosphodiester bond of a specific dinucleotide and remains bound to the 5'-end of the nick site.


Pssm-ID: 410988  Cd Length: 196  Bit Score: 126.22  E-value: 1.16e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433  29 AAAEHNLRLRTQA--NIDADRSHLNQILYNPLGIDSNDAtsFQRKLSEHYSslGIKIKAENTLAFEYVATASPKFFEGKT 106
Cdd:cd17242    14 GALRHNERERETEnpNIDPSRTHLNYELVGPGKKDYAEA--IKERLEELGK--KKKIRKDAVLAVEFILTASPEFFEGLD 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433 107 TEQIGKWAYHQVEFMRKEF-ADKLQFAVLHLDETTPHIHFFVSteqrsikkyknrygdCEKETWSLNSEKI--NPEYLRT 183
Cdd:cd17242    90 PEEIEEFFKDALEFLKERFgEENIVSAVVHLDETTPHLHAVVV---------------PITEDGRLSAKELfgGRKKLRE 154

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 1359830433 184 LQDNYAKSN-KIFGLSRGAKYSKLKNTTLKDFYRTMKK 220
Cdd:cd17242   155 LQDDYAEAVaKKGGLERGIKGSKAKHKTKEKELKAKHE 192
MobV NF041497
MobV family relaxase;
29-208 3.06e-22

MobV family relaxase;


Pssm-ID: 469385  Cd Length: 187  Bit Score: 92.63  E-value: 3.06e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433  29 AAAEHNLRL---RTQANIDADRSHLNQILYNPLGIDSNDATSFQRKLSEHYSSLgIKIKAENTLAFEYVATASPKFFEGK 105
Cdd:NF041497   18 GSQEHNEREtenRTNPNADPSRTHLNFELVNGPDGPIDYLEAIKERIAENAGIK-RKIRKDAVRAVEFILTGSPEFFKEL 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433 106 TTE-QIGKWAYHQVEFMRKEF-ADKLQFAVLHLDETTPHIHF-FVSTEQRSIKKYKnrygdceketwslnsEKINPEYLR 182
Cdd:NF041497   97 ADPgELKEWFEDNYDFLADRYgEENIVSAVVHLDETTPHIHAtVVPITEDGRLSAK---------------DVFGRKKLS 161

                         170       180
                  ....*....|....*....|....*.
gi 1359830433 183 TLQDNYAKSNKIFGLSRGAKYSKLKN 208
Cdd:NF041497  162 QLQDDYAEAMKKYGLERGISGSEAKH 187
Mob_Pre pfam01076
Plasmid recombination enzyme; With some plasmids, recombination can occur in a site specific ...
 33-215 5.17e-14

Plasmid recombination enzyme; With some plasmids, recombination can occur in a site specific manner that is independent of RecA. In such cases, the recombination event requires another protein called Pre. Pre is a plasmid recombination enzyme. This protein is also known as Mob (conjugative mobilization). This family is also known as Mob-V. One of the family members, MobM, is encoded by a promiscuous plasmid actively involved in the spread of antibiotic resistance. Homologs of MobM are found in many plasmids and other mobile genetic elements of pathogenic bacteria, including S. aureus. MobM is a metal-dependent nuclease that uses histidine nitrogen for the nucleophilic attack on the scissile phosphate. Furthermore, in contrast to other DNA-processing enzymes, MobM is a histidine relaxase, a DNA-breaking and -joining enzyme, that operates through a phosphorus-nitrogen protein-DNA adduct for cell-to-cell DNA transfer. Mutational analysis indicate that the H(N/D)(Q/E)R N-terminal motif of MobM plays a crucial role in the cleavage and generation of stable DNA-protein adducts.


Pssm-ID: 395854  Cd Length: 195  Bit Score: 70.04  E-value: 5.17e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433  33 HNLRLRTQA---NIDADRSHLNQILYNPLGIDsndatsFQRKLSEHYSSLGI---KIKAENTLAFEYVATASPKFFEGKT 106
Cdd:pfam01076  22 HNQRENETYdnkDIDPERSGLNYDLVNDKQIR------YDEDIKDYIKENQKskrKIRKDAVLENEILITSDPDFFEQLT 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1359830433 107 TEQIGKWAYHQVEFMRKEF-ADKLQFAVLHLDETTPHIHFFVSTeqrsikkyknrygdcEKETWSLNSEKI--NPEYLRT 183
Cdd:pfam01076  96 YEEAKEFFETAFQFFEERYgKENVLYAVVHLDEATPHMHMGVVP---------------GTEDGRLSAKAIfgNRKELIA 160

                         170       180       190
                  ....*....|....*....|....*....|....
gi 1359830433 184 LQDNYAK--SNKIFGLSRGAKYSKLKNTTLKDFY 215
Cdd:pfam01076 161 LQDRFPEymGLKGFDLERGEPGSERKHLGTNEYK 194
P2X_receptor pfam00864
ATP P2X receptor;
163-227 8.45e-03

ATP P2X receptor;


Pssm-ID: 459968  Cd Length: 362  Bit Score: 37.92  E-value: 8.45e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1359830433 163 DCEKETWSlnsEKINPEY-LRTLQDNYAKSNKIFGLS-RGAKYSKLKNTTLkdfYRTMKKVFGYSFD 227
Cdd:pfam00864 248 DCDLDSDR---SHCNPKYsFRRLDGKNFNNAVSPGYNfRFAKYYKEKNGVE---RRTLIKAYGIRFD 308
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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