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Conserved domains on  [gi|1171011020|ref|WP_080572022|]
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MULTISPECIES: polynucleotide adenylyltransferase PcnB [Haemophilus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
pcnB super family cl36036
poly(A) polymerase I; Provisional
 30-449 0e+00

poly(A) polymerase I; Provisional


The actual alignment was detected with superfamily member PRK11623:

Pssm-ID: 236939 [Multi-domain]  Cd Length: 472  Bit Score: 540.88  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  30 INPRMFSRNAITVVEKLQRQGYEAYIVGGCLRDLLLGKHPKDFDVATNARPDQIQAVFqRQCRLVGRRFRLAHIMFGRDV 109
Cdd:PRK11623   46 ISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLF-RNCRLVGRRFRLAHVMFGPEI 124

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 110 IEVATFRANHSDARSE-NQAKQSDEGMLLRDNVYGTIEQDAERRDFTVNALYYNPQDNTLRDYFNGIDDLKNGKLRLIGD 188
Cdd:PRK11623  125 IEVATFRGHHEGNESDrNTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKEGVIRLIGN 204

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 189 PVTRYQEDPVRMLRSVRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLKLLQSGNGVKTYKLLRQYGLFEQLFP 268
Cdd:PRK11623  205 PETRYREDPVRMLRAVRFAAKLDMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFP 284

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 269 SLTPYFTEKEDSLAERMILTSLNSTDERIADKLRINPAFLFAAFFWYPLREKVDVLKNEGGLNNHDAYALAGNEVLEQLC 348
Cdd:PRK11623  285 TITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLEHAQKIAQESGLAYYDAFALAMNDVLDEAC 364

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 349 RTLAAPRRHTAVIRDIWMLQLQLLKRTGSYPARTMEHQKFRAAFDLLAMRAEVEGG-ETVELAKWWHEYQLSNQEQRRQL 427
Cdd:PRK11623  365 RSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLALRAEVENNaELQRLAQWWGEFQVSAPPQQKGM 444

                         410       420
                  ....*....|....*....|..
gi 1171011020 428 VQEQQKlHPAPKKKYYRRRKPK 449
Cdd:PRK11623  445 LNELDD-DPAPRRRTRRPRKRA 465
 
Name Accession Description Interval E-value
pcnB PRK11623
poly(A) polymerase I; Provisional
 30-449 0e+00

poly(A) polymerase I; Provisional


Pssm-ID: 236939 [Multi-domain]  Cd Length: 472  Bit Score: 540.88  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  30 INPRMFSRNAITVVEKLQRQGYEAYIVGGCLRDLLLGKHPKDFDVATNARPDQIQAVFqRQCRLVGRRFRLAHIMFGRDV 109
Cdd:PRK11623   46 ISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLF-RNCRLVGRRFRLAHVMFGPEI 124

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 110 IEVATFRANHSDARSE-NQAKQSDEGMLLRDNVYGTIEQDAERRDFTVNALYYNPQDNTLRDYFNGIDDLKNGKLRLIGD 188
Cdd:PRK11623  125 IEVATFRGHHEGNESDrNTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKEGVIRLIGN 204

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 189 PVTRYQEDPVRMLRSVRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLKLLQSGNGVKTYKLLRQYGLFEQLFP 268
Cdd:PRK11623  205 PETRYREDPVRMLRAVRFAAKLDMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFP 284

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 269 SLTPYFTEKEDSLAERMILTSLNSTDERIADKLRINPAFLFAAFFWYPLREKVDVLKNEGGLNNHDAYALAGNEVLEQLC 348
Cdd:PRK11623  285 TITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLEHAQKIAQESGLAYYDAFALAMNDVLDEAC 364

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 349 RTLAAPRRHTAVIRDIWMLQLQLLKRTGSYPARTMEHQKFRAAFDLLAMRAEVEGG-ETVELAKWWHEYQLSNQEQRRQL 427
Cdd:PRK11623  365 RSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLALRAEVENNaELQRLAQWWGEFQVSAPPQQKGM 444

                         410       420
                  ....*....|....*....|..
gi 1171011020 428 VQEQQKlHPAPKKKYYRRRKPK 449
Cdd:PRK11623  445 LNELDD-DPAPRRRTRRPRKRA 465
pcnB TIGR01942
poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as ...
 23-441 3.17e-155

poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as plasmid copy number protein). These enzymes sequentially add adenosine nucleotides to the 3' end of RNAs, targeting them for degradation by the cell. This was originally described for anti-sense RNAs, but was later demonstrated for mRNAs as well. Members of this family are as yet limited to the gamma- and beta-proteobacteria, with putative members in the Chlamydiacae and spirochetes. This family has homology to tRNA nucleotidyltransferase (cca).


Pssm-ID: 130997 [Multi-domain]  Cd Length: 410  Bit Score: 446.17  E-value: 3.17e-155
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  23 VKASHFGINPRMFSRNAITVVEKLQRQGYEAYIVGGCLRDLLLGKHPKDFDVATNARPDQIQAVFqRQCRLVGRRFRLAH 102
Cdd:TIGR01942   2 IPESEHNIPRQSFSAHALNVVERLKGAGYQAYIVGGAVRDLLLGIEPKDFDVVTSATPEEVRKLF-RNSRIVGRRFRLVH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 103 IMFGRDVIEVATFRANHsdarsenQAKQSDEGMLLRDNVYGTIEQDAERRDFTVNALYYNPQDNTLRDYFNGIDDLKNGK 182
Cdd:TIGR01942  81 VSFGRQIIEVATFRSGH-------KSSVNAEGRILKDNVYGTLEEDAWRRDFTVNALYYDPSREVIIDYVGGMEDLKNRR 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 183 LRLIGDPVTRYQEDPVRMLRSVRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLKLLQSGNGVKTYKLLRQYGL 262
Cdd:TIGR01942 154 LRLIGDPRSRYQEDPVRMLRALRFSVKLEFTIDESTARPIRESAPLLKGIPPARLFEEILKLLFSGRSAALFRMLCGYQL 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 263 FEQLFPSLTPYFTEKEDSLAERMILTSLNSTDERIADKLRINPAFLFAAFFWYPLREKVDVLKNEgGLNNHDAYALAGNE 342
Cdd:TIGR01942 234 LEPLFPSVAYALRESPKFESAFTVQALVNDTDFRVKRDKPVTPAFLYAALLWPGLVFRVADAPDQ-GVMPYPAVFDAIRD 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 343 VLEQLCRTLAAPRRHTAVIRDIWMLQLQLLKRTGSYPARTMEHQKFRAAFDLLAMRaEVEGGETVELAKWWHEYQLSNQE 422
Cdd:TIGR01942 313 FVEEQCAPISIPKRFSIPTREIWQMQLRLERRSGMRARKLLGHARFRAAYDLLLLR-EVCGRENAVLGEWWTPYQLASEE 391

                         410
                  ....*....|....*....
gi 1171011020 423 QRRQLVQEQQKlHPAPKKK 441
Cdd:TIGR01942 392 ERTEMTRSAAR-HEGNRGR 409
PcnB COG0617
tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and ...
 36-418 6.08e-117

tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and biogenesis]; tRNA nucleotidyltransferase/poly(A) polymerase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440382 [Multi-domain]  Cd Length: 391  Bit Score: 347.57  E-value: 6.08e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  36 SRNAITVVEKLQRQGYEAYIVGGCLRDLLLGKHPKDFDVATNARPDQIQAVFQRQ--CRLVGRRFRLAHIMFGRDVIEVA 113
Cdd:COG0617     3 SPNALKVLEALEEAGFEAYLVGGAVRDLLLGRPPKDIDIVTVATPEEVAALFRKAlrTVPVGRDFGTVTVVFGGEKIEVA 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 114 TFRANHSDARSENQakqsdegmllrDNVYG-TIEQDAERRDFTVNALYYNPQDNTLRDYFNGIDDLKNGKLRLIGDPVTR 192
Cdd:COG0617    83 TARTERYYGDGRRP-----------FVEFGdTLEEDLARRDFTINALAYDLNDGELIDPFGGLADLEARVIRTVGDPEER 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 193 YQEDPVRMLRSVRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLKLLQSGNGVKTYKLLRQYGLFEQLfpsltp 272
Cdd:COG0617   152 FREDPLRILRAVRFAARLGFTIEPETLAAIREMAGLLDRLSAERVWDELLKLLLSPHPSRGLELLRETGLLEVL------ 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 273 yftekedslaermiltslnstderiadklrinpAFLFAAFFWyplrekvDVLK---NEGGLNNHDAYALAGNEVLEQLCR 349
Cdd:COG0617   226 ---------------------------------ALRLAALLH-------DLGKpatREDGLPTFHGHEEAGAELAEALLK 265

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1171011020 350 TLAAPRRHTAVIRDIWMLQLQLLKRTGSYPA---RTMEHqKFRAAFDLLAMRAEVEGGETVE------LAKWWHEYQL 418
Cdd:COG0617   266 RLRLPNRERKLVRELVELHLRFHGLGELRDSavrRLLER-GPEALEDLLLLRENGLEYPELQerlaelLEAAWRRFQP 342
PolyA_pol pfam01743
Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, ...
 53-184 1.17e-50

Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, such as Poly(A) polymerase, which adds the poly (A) tail to mRNA EC:2.7.7.19. This family also includes the tRNA nucleotidyltransferase that adds the CCA to the 3' of the tRNA EC:2.7.7.25. This family is part of the nucleotidyltransferase superfamily.


Pssm-ID: 396348 [Multi-domain]  Cd Length: 126  Bit Score: 167.84  E-value: 1.17e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  53 AYIVGGCLRDLLLGKHPKDFDVATNARPDQIQAVFQR---QCRLVGRRFRLAHIMFGRDVIEVATFRANH--SDARSENQ 127
Cdd:pfam01743   1 LYIVGGAVRDLLLGKTPKDVDIATDATPEQVATLFRRrriVHLLSGIEFGTIHVIFGNQILEVATFRIEFdeSDFRNPRS 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1171011020 128 AKQSdegmllrdnvyGTIEQDAERRDFTVNALYYNPQDNTLRDYFNGIDDLKNGKLR 184
Cdd:pfam01743  81 EEYT-----------GTLEEDAKRRDFTINALAYNPNSGEVIDYFGGIKDLKSGVIR 126
NT_ClassII-CCAase cd05398
Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the ...
 38-180 1.04e-40

Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This Class II group is comprised mainly of eubacterial and eukaryotic enzymes and includes Bacillus stearothermophilus CCAase, Escherichia coli poly(A) polymerase I, human mitochondrial CCAase, and Saccharomyces cerevisiae CCAase (CCA1). CCA-adding enzymes have a single catalytic pocket, which recognizes both ATP and CTP substrates. Included in this subgroup are CC- and A-adding enzymes from various ancient species of bacteria such as Aquifex aeolicus; these enzymes collaborate to add CCA to tRNAs. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family. Escherichia coli CCAase is related to this group but has not been included in this alignment as this enzyme lacks the N-terminal helix conserved in the remainder of the NT superfamily.


Pssm-ID: 143388 [Multi-domain]  Cd Length: 139  Bit Score: 141.96  E-value: 1.04e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  38 NAITVVEKLQR-QGYEAYIVGGCLRDLLLGKHPKDFDVATNAR-PDQIQAVFQRQ-CRLVGR--RFRLAHIMFGRDVIEV 112
Cdd:cd05398     3 ELLKLLRELKKaLGYEAYLVGGAVRDLLLGRPPKDIDIATDADgPEFAEALFKKIgGRVVGLgeEFGTATVVINGLTIDV 82

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1171011020 113 ATFRAnhsdarsenqAKQSDEGMLLRDNVYgTIEQDAERRDFTVNALYYNPQDNTLRDYFNGIDDLKN 180
Cdd:cd05398    83 ATLRT----------ETYTDPGRRPPVVGF-TIEEDLLRRDFTINAMAYDLDDGELIDPFGGLKDLEN 139
 
Name Accession Description Interval E-value
pcnB PRK11623
poly(A) polymerase I; Provisional
 30-449 0e+00

poly(A) polymerase I; Provisional


Pssm-ID: 236939 [Multi-domain]  Cd Length: 472  Bit Score: 540.88  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  30 INPRMFSRNAITVVEKLQRQGYEAYIVGGCLRDLLLGKHPKDFDVATNARPDQIQAVFqRQCRLVGRRFRLAHIMFGRDV 109
Cdd:PRK11623   46 ISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLF-RNCRLVGRRFRLAHVMFGPEI 124

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 110 IEVATFRANHSDARSE-NQAKQSDEGMLLRDNVYGTIEQDAERRDFTVNALYYNPQDNTLRDYFNGIDDLKNGKLRLIGD 188
Cdd:PRK11623  125 IEVATFRGHHEGNESDrNTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKEGVIRLIGN 204

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 189 PVTRYQEDPVRMLRSVRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLKLLQSGNGVKTYKLLRQYGLFEQLFP 268
Cdd:PRK11623  205 PETRYREDPVRMLRAVRFAAKLDMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFP 284

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 269 SLTPYFTEKEDSLAERMILTSLNSTDERIADKLRINPAFLFAAFFWYPLREKVDVLKNEGGLNNHDAYALAGNEVLEQLC 348
Cdd:PRK11623  285 TITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLEHAQKIAQESGLAYYDAFALAMNDVLDEAC 364

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 349 RTLAAPRRHTAVIRDIWMLQLQLLKRTGSYPARTMEHQKFRAAFDLLAMRAEVEGG-ETVELAKWWHEYQLSNQEQRRQL 427
Cdd:PRK11623  365 RSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLALRAEVENNaELQRLAQWWGEFQVSAPPQQKGM 444

                         410       420
                  ....*....|....*....|..
gi 1171011020 428 VQEQQKlHPAPKKKYYRRRKPK 449
Cdd:PRK11623  445 LNELDD-DPAPRRRTRRPRKRA 465
pcnB TIGR01942
poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as ...
 23-441 3.17e-155

poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as plasmid copy number protein). These enzymes sequentially add adenosine nucleotides to the 3' end of RNAs, targeting them for degradation by the cell. This was originally described for anti-sense RNAs, but was later demonstrated for mRNAs as well. Members of this family are as yet limited to the gamma- and beta-proteobacteria, with putative members in the Chlamydiacae and spirochetes. This family has homology to tRNA nucleotidyltransferase (cca).


Pssm-ID: 130997 [Multi-domain]  Cd Length: 410  Bit Score: 446.17  E-value: 3.17e-155
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  23 VKASHFGINPRMFSRNAITVVEKLQRQGYEAYIVGGCLRDLLLGKHPKDFDVATNARPDQIQAVFqRQCRLVGRRFRLAH 102
Cdd:TIGR01942   2 IPESEHNIPRQSFSAHALNVVERLKGAGYQAYIVGGAVRDLLLGIEPKDFDVVTSATPEEVRKLF-RNSRIVGRRFRLVH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 103 IMFGRDVIEVATFRANHsdarsenQAKQSDEGMLLRDNVYGTIEQDAERRDFTVNALYYNPQDNTLRDYFNGIDDLKNGK 182
Cdd:TIGR01942  81 VSFGRQIIEVATFRSGH-------KSSVNAEGRILKDNVYGTLEEDAWRRDFTVNALYYDPSREVIIDYVGGMEDLKNRR 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 183 LRLIGDPVTRYQEDPVRMLRSVRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLKLLQSGNGVKTYKLLRQYGL 262
Cdd:TIGR01942 154 LRLIGDPRSRYQEDPVRMLRALRFSVKLEFTIDESTARPIRESAPLLKGIPPARLFEEILKLLFSGRSAALFRMLCGYQL 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 263 FEQLFPSLTPYFTEKEDSLAERMILTSLNSTDERIADKLRINPAFLFAAFFWYPLREKVDVLKNEgGLNNHDAYALAGNE 342
Cdd:TIGR01942 234 LEPLFPSVAYALRESPKFESAFTVQALVNDTDFRVKRDKPVTPAFLYAALLWPGLVFRVADAPDQ-GVMPYPAVFDAIRD 312

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 343 VLEQLCRTLAAPRRHTAVIRDIWMLQLQLLKRTGSYPARTMEHQKFRAAFDLLAMRaEVEGGETVELAKWWHEYQLSNQE 422
Cdd:TIGR01942 313 FVEEQCAPISIPKRFSIPTREIWQMQLRLERRSGMRARKLLGHARFRAAYDLLLLR-EVCGRENAVLGEWWTPYQLASEE 391

                         410
                  ....*....|....*....
gi 1171011020 423 QRRQLVQEQQKlHPAPKKK 441
Cdd:TIGR01942 392 ERTEMTRSAAR-HEGNRGR 409
PcnB COG0617
tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and ...
 36-418 6.08e-117

tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and biogenesis]; tRNA nucleotidyltransferase/poly(A) polymerase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440382 [Multi-domain]  Cd Length: 391  Bit Score: 347.57  E-value: 6.08e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  36 SRNAITVVEKLQRQGYEAYIVGGCLRDLLLGKHPKDFDVATNARPDQIQAVFQRQ--CRLVGRRFRLAHIMFGRDVIEVA 113
Cdd:COG0617     3 SPNALKVLEALEEAGFEAYLVGGAVRDLLLGRPPKDIDIVTVATPEEVAALFRKAlrTVPVGRDFGTVTVVFGGEKIEVA 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 114 TFRANHSDARSENQakqsdegmllrDNVYG-TIEQDAERRDFTVNALYYNPQDNTLRDYFNGIDDLKNGKLRLIGDPVTR 192
Cdd:COG0617    83 TARTERYYGDGRRP-----------FVEFGdTLEEDLARRDFTINALAYDLNDGELIDPFGGLADLEARVIRTVGDPEER 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 193 YQEDPVRMLRSVRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLKLLQSGNGVKTYKLLRQYGLFEQLfpsltp 272
Cdd:COG0617   152 FREDPLRILRAVRFAARLGFTIEPETLAAIREMAGLLDRLSAERVWDELLKLLLSPHPSRGLELLRETGLLEVL------ 225

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 273 yftekedslaermiltslnstderiadklrinpAFLFAAFFWyplrekvDVLK---NEGGLNNHDAYALAGNEVLEQLCR 349
Cdd:COG0617   226 ---------------------------------ALRLAALLH-------DLGKpatREDGLPTFHGHEEAGAELAEALLK 265

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1171011020 350 TLAAPRRHTAVIRDIWMLQLQLLKRTGSYPA---RTMEHqKFRAAFDLLAMRAEVEGGETVE------LAKWWHEYQL 418
Cdd:COG0617   266 RLRLPNRERKLVRELVELHLRFHGLGELRDSavrRLLER-GPEALEDLLLLRENGLEYPELQerlaelLEAAWRRFQP 342
PRK13299 PRK13299
tRNA CCA-pyrophosphorylase; Provisional
 31-283 2.18e-51

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 237339 [Multi-domain]  Cd Length: 394  Bit Score: 178.11  E-value: 2.18e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  31 NPRMFSRnAITVVEKLQRQGYEAYIVGGCLRDLLLGKHPKDFDVATNARPDQIQAVFQRQCRlVGRRFRLAHIMFGRDVI 110
Cdd:PRK13299    2 MPSEFQK-ALPILEKIKEAGFEAYFVGGSVRDYLLGRPIHDVDIATSAYPEEVKAIFPRTVD-VGIEHGTVLVLENGEEY 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 111 EVATFRA--NHSDAR--SENQAKQSdegmllrdnvygtIEQDAERRDFTVNALYYNpQDNTLRDYFNGIDDLKNGKLRLI 186
Cdd:PRK13299   80 EVTTFRTesEYVDYRrpSEVTFVRS-------------LEEDLKRRDFTINAIAMD-ENGEIIDLFDGLEDLKNRLIRAV 145

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 187 GDPVTRYQEDPVRMLRSVRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLKLLQSGNGVKTYKLLRQYGLFEQL 266
Cdd:PRK13299  146 GNAEERFQEDALRMMRAVRFASQLGFDLETETFEAMKTQAPLLEKISVERIFVEFEKLLLGPFWRKGLKLLIETGLYNYL 225

                         250
                  ....*....|....*..
gi 1171011020 267 fpsltPYFTEKEDSLAE 283
Cdd:PRK13299  226 -----PGLKGKEENLLK 237
PolyA_pol pfam01743
Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, ...
 53-184 1.17e-50

Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, such as Poly(A) polymerase, which adds the poly (A) tail to mRNA EC:2.7.7.19. This family also includes the tRNA nucleotidyltransferase that adds the CCA to the 3' of the tRNA EC:2.7.7.25. This family is part of the nucleotidyltransferase superfamily.


Pssm-ID: 396348 [Multi-domain]  Cd Length: 126  Bit Score: 167.84  E-value: 1.17e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  53 AYIVGGCLRDLLLGKHPKDFDVATNARPDQIQAVFQR---QCRLVGRRFRLAHIMFGRDVIEVATFRANH--SDARSENQ 127
Cdd:pfam01743   1 LYIVGGAVRDLLLGKTPKDVDIATDATPEQVATLFRRrriVHLLSGIEFGTIHVIFGNQILEVATFRIEFdeSDFRNPRS 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1171011020 128 AKQSdegmllrdnvyGTIEQDAERRDFTVNALYYNPQDNTLRDYFNGIDDLKNGKLR 184
Cdd:pfam01743  81 EEYT-----------GTLEEDAKRRDFTINALAYNPNSGEVIDYFGGIKDLKSGVIR 126
PolyA_pol_arg_C pfam12626
Polymerase A arginine-rich C-terminus; The C-terminus of polymerase A in E coli is ...
 328-446 5.87e-43

Polymerase A arginine-rich C-terminus; The C-terminus of polymerase A in E coli is arginine-rich and is necessary for full functioning of the enzyme.


Pssm-ID: 463647 [Multi-domain]  Cd Length: 116  Bit Score: 146.96  E-value: 5.87e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 328 GGLNNHDAYALAGNEVLEQLCRTLAAPRRHTAVIRDIWMLQLQLLKRTGSYPARTMEHQKFRAAFDLLAMRAEVeGGETV 407
Cdd:pfam12626   1 QGLPPYDALQLAADEVLDEQCQRVAIPRRFTAPMREIWLLQPRLEKRQGKRPFRLLEHPRFRAAYDFLLLRAES-GEVLQ 79

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1171011020 408 ELAKWWHEYQLSNQEQRRQLVQEQQKLHPAPKKKyyRRR 446
Cdd:pfam12626  80 ELAEWWTRFQEADEAEREAMIAELGADAAKKKRR--RRR 116
NT_ClassII-CCAase cd05398
Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the ...
 38-180 1.04e-40

Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This Class II group is comprised mainly of eubacterial and eukaryotic enzymes and includes Bacillus stearothermophilus CCAase, Escherichia coli poly(A) polymerase I, human mitochondrial CCAase, and Saccharomyces cerevisiae CCAase (CCA1). CCA-adding enzymes have a single catalytic pocket, which recognizes both ATP and CTP substrates. Included in this subgroup are CC- and A-adding enzymes from various ancient species of bacteria such as Aquifex aeolicus; these enzymes collaborate to add CCA to tRNAs. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family. Escherichia coli CCAase is related to this group but has not been included in this alignment as this enzyme lacks the N-terminal helix conserved in the remainder of the NT superfamily.


Pssm-ID: 143388 [Multi-domain]  Cd Length: 139  Bit Score: 141.96  E-value: 1.04e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  38 NAITVVEKLQR-QGYEAYIVGGCLRDLLLGKHPKDFDVATNAR-PDQIQAVFQRQ-CRLVGR--RFRLAHIMFGRDVIEV 112
Cdd:cd05398     3 ELLKLLRELKKaLGYEAYLVGGAVRDLLLGRPPKDIDIATDADgPEFAEALFKKIgGRVVGLgeEFGTATVVINGLTIDV 82

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1171011020 113 ATFRAnhsdarsenqAKQSDEGMLLRDNVYgTIEQDAERRDFTVNALYYNPQDNTLRDYFNGIDDLKN 180
Cdd:cd05398    83 ATLRT----------ETYTDPGRRPPVVGF-TIEEDLLRRDFTINAMAYDLDDGELIDPFGGLKDLEN 139
PolyA_pol_RNAbd pfam12627
Probable RNA and SrmB- binding site of polymerase A; This region encompasses much of the RNA ...
 211-274 3.70e-21

Probable RNA and SrmB- binding site of polymerase A; This region encompasses much of the RNA and SrmB binding motifs on polymerase A.


Pssm-ID: 463648 [Multi-domain]  Cd Length: 64  Bit Score: 86.38  E-value: 3.70e-21
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1171011020 211 DMFLEKPSEQPIRELAPLLKNIPPARLFDESLKLLQSGNGVKTYKLLRQYGLFEQLFPSLTPYF 274
Cdd:pfam12627   1 GFTIEPETREAIRKLAPLLKKISPERIFEELLKLLLSGHPERGLELLRETGLLEYLFPELAAAL 64
PRK13296 PRK13296
CCA tRNA nucleotidyltransferase;
52-299 1.54e-17

CCA tRNA nucleotidyltransferase;


Pssm-ID: 106256 [Multi-domain]  Cd Length: 360  Bit Score: 83.88  E-value: 1.54e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  52 EAYIVGGCLRDLLLGKHPKDFD-VATNARPDQ-IQAVFQRqcrlvgrrfrlahIMFGRDVIEVATFRANHSDARSENQAK 129
Cdd:PRK13296    2 KFYLVGGAVRDMLLGITPKDKDwVVVGATEDEmLANGFIK-------------IAANFPVFIHPQTKQEYALARSEKKTA 68

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 130 QSDEGMLLRDNVYGTIEQDAERRDFTVNALYYNpQDNTLRDYFNGIDDLKNGKLRLIGDPvtrYQEDPVRMLRSVRFMAK 209
Cdd:PRK13296   69 SGYHGFEVNFSKYITLEDDLKRRDLTINSIAID-QNNKVIDPFNGQADLQNRILRHTSIA---FIEDPLRVVRLARFKAQ 144

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 210 LDMFLEKPSEQPIRELAPLLKN-----IPPARLFDESLKLLQsgNGVKTYKLLRQYGLFEQLFPSLT---------PYF- 274
Cdd:PRK13296  145 LSNFNFSIAQEMLALIKELVKTgelnhLTRERLHIEFVKALN--NPKIFFTTLKELEALKIIFPNIScilplipnkSFFe 222

                         250       260
                  ....*....|....*....|....*..
gi 1171011020 275 --TEKEDSLAERMILTSLNSTDERIAD 299
Cdd:PRK13296  223 npIYKGSNINEKITLCLLKIPQQQLDD 249
PRK13297 PRK13297
tRNA CCA-pyrophosphorylase; Provisional
 50-270 5.85e-16

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 139469 [Multi-domain]  Cd Length: 364  Bit Score: 78.88  E-value: 5.85e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  50 GYEAYIVGGCLRDLLLGKHPKDFD-VATNARPDQIQavfqrqcrlvgrrfRLAHIMFGRD--VIEVATFRANHSDARSEN 126
Cdd:PRK13297   11 GLQVYIVGGAVRDALLGLPAGDRDwVVVGATPEDMA--------------RRGFIPVGGDfpVFLHPRTKEEYALARTER 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 127 QAKQSDEGMLLRDNVYGTIEQDAERRDFTVNALYYNPQdNTLRDYFNGIDDLKNGKLRLIGDPvtrYQEDPVRMLRSVRF 206
Cdd:PRK13297   77 KSGRGYKGFTFYTGADVTLEQDLQRRDLTVNAIARTPQ-GELVDPLDGVADVRARVLRHVGEA---FAEDPVRILRLGRF 152

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1171011020 207 MAKLDMFLEKPSE-QPIREL--APLLKNIPPARLFDESLKLLQSGNGVKTYKLLRQYGLFEQLFPSL 270
Cdd:PRK13297  153 AARFGDFSIAPETmQLCRRMveAGEADALVPERVWKEVSRGLMAQAPSRMLDVLARAGALARVMPEL 219
PRK13298 PRK13298
tRNA CCA-pyrophosphorylase; Provisional
 52-275 4.85e-13

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 237338 [Multi-domain]  Cd Length: 417  Bit Score: 70.53  E-value: 4.85e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  52 EAYIVGGCLRDLLLGKHPKDFD-VATNARPdqiQAVFQRQCRLVGRRFrlahimfgrDVIEVATFRANHSDARSENQAKQ 130
Cdd:PRK13298    2 KIYLVGGAVRDSLLNLPVKDKDwVVVGGTP---KILLSINFQQVGKDF---------PVFLHPETHEEYALARTERKSGV 69

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 131 SDEGMLLRDNVYGTIEQDAERRDFTVNALYYNPQDNTLrDYFNGIDDLKNGKLRLIGDPvtrYQEDPVRMLRSVRFMAKL 210
Cdd:PRK13298   70 GYTGFITDTSSDVTLEEDLIRRDLTINAIAQDENGNYI-DPFQGKKDIQLRLLRHVSES---FIEDPLRVLRVARFAALL 145

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1171011020 211 DMFLEKPSEQPIRELAPL-----LKNIPPARLFDESLKLLQSGNGVKTYKLLRQYGLFEQLFPSL-----TPYFT 275
Cdd:PRK13298  146 VHLGFKIAKETMILMCIMvkkheLLYLTPERIWNETEKALKTDNPHVYFQVLYECNALKFLFPEIdflyeKPYFL 220
cca PRK10885
multifunctional CCA addition/repair protein;
54-210 1.18e-12

multifunctional CCA addition/repair protein;


Pssm-ID: 182810 [Multi-domain]  Cd Length: 409  Bit Score: 69.11  E-value: 1.18e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020  54 YIVGGCLRDLLLGKHPKDFD-VATNARPDQIQAVFQRQcrlVGRRFrlahimfgrdvievATF-----RANHSDARSEnq 127
Cdd:PRK10885    4 YLVGGAVRDALLGLPVKDRDwVVVGATPEEMLAQGYQQ---VGKDF--------------PVFlhpktHEEYALARTE-- 64

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1171011020 128 aKQSDEGmllrdnvYG----------TIEQDAERRDFTVNALYYNPQDNtLRDYFNGIDDLKNGKLRLIGDPvtrYQEDP 197
Cdd:PRK10885   65 -RKSGRG-------YTgftcyaapdvTLEEDLIRRDLTINAMAQDDDGE-LIDPYGGQRDLEARLLRHVSPA---FAEDP 132

                         170
                  ....*....|...
gi 1171011020 198 VRMLRSVRFMAKL 210
Cdd:PRK10885  133 LRVLRVARFAARF 145
PHA01806 PHA01806
hypothetical protein
 53-93 1.18e-03

hypothetical protein


Pssm-ID: 222838  Cd Length: 200  Bit Score: 39.87  E-value: 1.18e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 1171011020  53 AYIVGGCLRDLLLGKHPKDFDVATnARPDQIQAVFQRQCRL 93
Cdd:PHA01806   38 VALAGGAARDLMHGAEPKDIDIAL-YGMDDRQAELLIGCIL 77
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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