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Conserved domains on  [gi|1159373959|ref|WP_079263671|]
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MULTISPECIES: thiaminase II [Campylobacter]

Protein Classification

TenA family protein( domain architecture ID 14443675)

TenA family protein such as Bacillus halodurans aminopyrimidine aminohydrolase, which catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, as part of the thiamine salvage pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 13-216 3.07e-76

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


:

Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 228.17  E-value: 3.07e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  13 KIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEgEMELHRAIL-S 91
Cdd:cd19367     1 PIWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILE-EMELHVKYCaE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  92 LGINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRLKNeNLKDHPYKEWILTYGSENF 171
Cdd:cd19367    80 WGISEEELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADPAT-KLEGNPYWSWIETYASDEY 158

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1159373959 172 QNEAKEFEDFVNSYTS-SVSAQKFQKLSEIFHTVTRLEVAFWEHSL 216
Cdd:cd19367   159 QEAVREGIELLEKLAAeRPSPARLEELVKIFATATRLEIGFWDMGL 204
 
Name Accession Description Interval E-value
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 13-216 3.07e-76

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 228.17  E-value: 3.07e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  13 KIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEgEMELHRAIL-S 91
Cdd:cd19367     1 PIWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILE-EMELHVKYCaE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  92 LGINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRLKNeNLKDHPYKEWILTYGSENF 171
Cdd:cd19367    80 WGISEEELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADPAT-KLEGNPYWSWIETYASDEY 158

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1159373959 172 QNEAKEFEDFVNSYTS-SVSAQKFQKLSEIFHTVTRLEVAFWEHSL 216
Cdd:cd19367   159 QEAVREGIELLEKLAAeRPSPARLEELVKIFATATRLEIGFWDMGL 204
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 1-219 2.94e-75

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 226.30  E-value: 2.94e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   1 MMLFSNLIKENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVE 80
Cdd:COG0819     1 MSFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  81 GEMELHRAILS-LGINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRLKNENlkdHPY 159
Cdd:COG0819    81 VELALHERYAAeLGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERPLPPD---HPY 157

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959 160 KEWILTYGSENFQNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWEHSLRME 219
Cdd:COG0819   158 AEWIETYASEEFQELVEWLIALLDRLAATASEAERERLEEAFRTASRLEYAFWDMAYRLE 217
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 12-217 1.86e-36

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 126.92  E-value: 1.86e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  12 QKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGEMELHRAILS 91
Cdd:TIGR04306   2 KPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLAE 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  92 -LGINADE-LDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYakigAEIINRLKNE-NLKDHP-YKEWILTYG 167
Cdd:TIGR04306  82 vLGISDEEyFQKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGY----GEAATKLKGKhTAPEHSvYHKWIETYS 157

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 1159373959 168 SENFQNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWEHSLR 217
Cdd:TIGR04306 158 SSWFREAVNEGENLLNHLAETSSPEELEKLKTIFAESCEYEYNFWTMAYE 207
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 14-218 3.31e-32

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 116.30  E-value: 3.31e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  14 IWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGEMELHRAIL-SL 92
Cdd:pfam03070   7 IWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRLAeAL 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  93 GINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRLKneNLKDHPYKEWILTYGSENFQ 172
Cdd:pfam03070  87 GLSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEKIR--ALEGPEYYEWVKTYASEYFR 164

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1159373959 173 NEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWEHSLRM 218
Cdd:pfam03070 165 AAVEEAKRLLDRLLAYVSDEQLEELQEIFLKALEFEYSFWTMALDA 210
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
14-217 2.15e-28

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 111.42  E-value: 2.15e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  14 IWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGEMELHRAILSlG 93
Cdd:PRK14713  331 IREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVESELHRSWLG-D 409

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  94 INADeldiKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRLKneNLKDHPYKEWILTYGSENFQN 173
Cdd:PRK14713  410 RDAD----TAPSPVTLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAELHARAG--NPDDHPYAEWLQTYADPEFAA 483

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 1159373959 174 EAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWEHSLR 217
Cdd:PRK14713  484 ATRRAIAFVDRAFRAASPAERAAMARAFLTACRYELEFFDQARR 527
 
Name Accession Description Interval E-value
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 13-216 3.07e-76

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 228.17  E-value: 3.07e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  13 KIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEgEMELHRAIL-S 91
Cdd:cd19367     1 PIWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILE-EMELHVKYCaE 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  92 LGINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRLKNeNLKDHPYKEWILTYGSENF 171
Cdd:cd19367    80 WGISEEELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADPAT-KLEGNPYWSWIETYASDEY 158

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1159373959 172 QNEAKEFEDFVNSYTS-SVSAQKFQKLSEIFHTVTRLEVAFWEHSL 216
Cdd:cd19367   159 QEAVREGIELLEKLAAeRPSPARLEELVKIFATATRLEIGFWDMGL 204
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 1-219 2.94e-75

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 226.30  E-value: 2.94e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   1 MMLFSNLIKENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVE 80
Cdd:COG0819     1 MSFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  81 GEMELHRAILS-LGINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRLKNENlkdHPY 159
Cdd:COG0819    81 VELALHERYAAeLGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERPLPPD---HPY 157

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959 160 KEWILTYGSENFQNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWEHSLRME 219
Cdd:COG0819   158 AEWIETYASEEFQELVEWLIALLDRLAATASEAERERLEEAFRTASRLEYAFWDMAYRLE 217
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 4-213 5.93e-54

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 171.97  E-value: 5.93e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   4 FSNLIKEN-QKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKEL-RFAmKFQNYIVEG 81
Cdd:cd19366     1 FSERLREKaKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETMgRFA-ELLHGTLNT 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  82 EMELHRA-ILSLGINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAeiinRLKNEN--LKDHP 158
Cdd:cd19366    80 EMDLHRQyAAEFGITEEELEATEPSPTTLAYTSYMLRTAQTGTLAELLAALLPCAWGYAEIGK----RLAEQGgaLEHNP 155

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1159373959 159 YKEWILTYGSENFQNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWE 213
Cdd:cd19366   156 YREWIEMYSSDEFTELADWLIDLLDELAEGKSEAELERLEEIFLTSSRYEYMFWD 210
TenA_C_HP1287-like cd19361
TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins ...
 7-213 1.75e-52

TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Helicobacter pylori TenA (HP1287) protein which is thought to catalyze a salvage reaction in thiamin metabolism, however its pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. HP1287 may contribute to stomach colonization and persistence.


Pssm-ID: 381696 [Multi-domain]  Cd Length: 212  Bit Score: 168.14  E-value: 1.75e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   7 LIKENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGEMELH 86
Cdd:cd19361     5 LYEAVEDIWDSYYEHPFVQGIADGTLDIEKFRFYMIQDYLYLLDYAKVFALGVAKAKDEEVMRFFADLINAILNEEMDIH 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  87 RAILS-LGINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRlkNENLKDHP-YKEWIL 164
Cdd:cd19361    85 RGYMKrLGITEEEIENTKPALDNLSYTSYMLSVAYEGGIAEILAAILSCSWSYEYIAKKLVER--YPAALEHEfYGEWVK 162

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 1159373959 165 TYGSENFQNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWE 213
Cdd:cd19361   163 GYSSEEYAEANQELIDLLDRLTEDISEEQIEKLEEIFVNCSRYELKFWD 211
TenA_C-like cd19369
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 13-213 1.67e-48

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381704 [Multi-domain]  Cd Length: 202  Bit Score: 157.78  E-value: 1.67e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  13 KIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGEMELHRAILS- 91
Cdd:cd19369     1 PIWEQYLEHPFIKELGEGTLDKEKFKNYLIQDSLYLKEYAKVFAMGIYKSRTMKEMQFFYSSLSFVNEDETATRIKYLKe 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  92 LGINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRLKnENLKDHPYKEWILTYGSENF 171
Cdd:cd19369    81 FGLTDEDIEKIEPLPENKAYTDYMLGIAKTGDVKEILMAVLPCMLSYYYIFKELVKKYK-DNLESNPYKDWIEDYASEEY 159

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 1159373959 172 QNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWE 213
Cdd:cd19369   160 AEYCKEWIDFADRLCENLSEEEKEKLKEIFRKASLYELKFWD 201
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 14-213 1.76e-46

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 152.50  E-value: 1.76e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  14 IWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAkELRFAMKFQNYIVEGEMELHRAIL-SL 92
Cdd:cd16099     1 IWDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARALALAAAKAPDL-ELRTFLAELINVLDDELELHEKLLaEL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  93 GINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGaeiiNRLKNENLKDHPYKEWILTYGSENFQ 172
Cdd:cd16099    80 GISEEDLSEAEPNPATLAYTNHLLRVAARGTPAEGLAALLPCYWSYGEIG----RRLAASLPEHPPYRFWIDFYASDEYQ 155

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 1159373959 173 NEAKEFEDFVNSYTSSVSAQKfQKLSEIFHTVTRLEVAFWE 213
Cdd:cd16099   156 ELVEELLQLLDQLAAAGEEEK-EELKEIFLTSLRYELMFWD 195
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 7-213 2.07e-39

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 134.56  E-value: 2.07e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   7 LIKENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGEMELH 86
Cdd:cd19365     4 LWEAIAPIYAAILAHPFIRELADGTLPREKFRFYLAQDALYLRDYARALALLAARAPDPEEQVFFARFAAGAIEVERELH 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  87 RAILS-LGINADeldiKDESLVNIAYSRYMLSVGENGDFldmLVALSA---CAIGYAKIGAeiinRLKNENLKDHPYKEW 162
Cdd:cd19365    84 RSFLGeFGIDAA----AEPSPVTLAYTSFLLATAATGPY---AVAVAAvlpCFWIYAEVGK----RLAAAASPNHPYQDW 152

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1159373959 163 ILTYGSENFQNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWE 213
Cdd:cd19365   153 IDTYSDEEFAEAVRRAIAIVDRLAAEASPEERARMLEAFLRASRLEWMFWD 203
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 12-217 1.86e-36

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 126.92  E-value: 1.86e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  12 QKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGEMELHRAILS 91
Cdd:TIGR04306   2 KPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLAE 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  92 -LGINADE-LDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYakigAEIINRLKNE-NLKDHP-YKEWILTYG 167
Cdd:TIGR04306  82 vLGISDEEyFQKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGY----GEAATKLKGKhTAPEHSvYHKWIETYS 157

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 1159373959 168 SENFQNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWEHSLR 217
Cdd:TIGR04306 158 SSWFREAVNEGENLLNHLAETSSPEELEKLKTIFAESCEYEYNFWTMAYE 207
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 4-213 7.81e-34

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 120.39  E-value: 7.81e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   4 FSN-LIKENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGE 82
Cdd:cd19360     1 FSEeLREEAQPILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSDTREDMRFFLEQIGFILNGE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  83 MELHRAILSL-GINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRLKNEnlKDHPYKE 161
Cdd:cd19360    81 SHAHQNLCEVaGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIEEGKPT--PDNPFYE 158

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1159373959 162 WILTYGSENFQNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWE 213
Cdd:cd19360   159 WIDFYADDEMDGLTDQLFARLDRLAEKASEEERERAKQAFLKSCQLEWRFWE 210
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 14-218 3.31e-32

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 116.30  E-value: 3.31e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  14 IWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGEMELHRAIL-SL 92
Cdd:pfam03070   7 IWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRLAeAL 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  93 GINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRLKneNLKDHPYKEWILTYGSENFQ 172
Cdd:pfam03070  87 GLSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEKIR--ALEGPEYYEWVKTYASEYFR 164

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1159373959 173 NEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWEHSLRM 218
Cdd:pfam03070 165 AAVEEAKRLLDRLLAYVSDEQLEELQEIFLKALEFEYSFWTMALDA 210
TenA_C_BsTenA-like cd19364
TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the ...
 4-213 1.80e-30

TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus subtilis TenA which has been shown to be a thiaminase II, catalyzing the hydrolysis of thiamine into HMP and 5-(2-hydroxyethyl)-4-methylthiazole (THZ), within thiamine metabolism. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381699 [Multi-domain]  Cd Length: 212  Bit Score: 111.51  E-value: 1.80e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   4 FSNLI-KENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGE 82
Cdd:cd19364     1 FTEELrEAADPLWQKSFEHPFIQGLADGTLPLETFRYYLIQDAYYLKHFAKLHALAAAKADDPAIKALLLEGAQGLAEGE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  83 MELHRAILS-LGINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEiinrlknenLKDHP--- 158
Cdd:cd19364    81 LALRETFFKeLGITEEEIAQTPPAPTAYHYVSHMYRQLNEGSVAEAVAALLPCYWLYQEIGER---------LADAGspv 151

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1159373959 159 --YKEWILTYGSENFQNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWE 213
Cdd:cd19364   152 plYQRWIDTYASDEFTESVQQQIDLVDRLAEEASEEEREKMKQAFLISSYYELQFWE 208
TenA_C_Bt3146-like cd19359
uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of ...
 7-213 1.14e-29

uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA-like proteins such as Bacteroides thetaiotaomicron Bt3146.


Pssm-ID: 381694 [Multi-domain]  Cd Length: 206  Bit Score: 109.36  E-value: 1.14e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   7 LIKENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRF-AMKFQNYIVEGEMEL 85
Cdd:cd19359     4 LWNDNEDLWDKALNNPFCQGMADGTLDLDGFGYYMVQDYYYCINYVRFKALRAAKAPDPDLLAFlAAKIKSYLDYAEDFL 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  86 HRAILSLGINADELDIKDESLVNiAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINrlkNENLKDHP-YKEWIl 164
Cdd:cd19359    84 KTCHIKLGIPDVVDGVKPSPALK-AYVDFERSVAESEDWFYLLVAMIPCIYLWYWLANQLNE---DPSDKNTNfYKTWI- 158

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1159373959 165 tygSENFQNE--AKEFEDFVNSYTsSVSAQKFQKLSEIFHTVTRLEVAFWE 213
Cdd:cd19359   159 ---EPNLPDPssAKQLEFFLNANA-AWSKIDREKANEIFRQAMQLEINFFN 205
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
14-217 2.15e-28

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 111.42  E-value: 2.15e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  14 IWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGEMELHRAILSlG 93
Cdd:PRK14713  331 IREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVESELHRSWLG-D 409

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  94 INADeldiKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRLKneNLKDHPYKEWILTYGSENFQN 173
Cdd:PRK14713  410 RDAD----TAPSPVTLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAELHARAG--NPDDHPYAEWLQTYADPEFAA 483

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 1159373959 174 EAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWEHSLR 217
Cdd:PRK14713  484 ATRRAIAFVDRAFRAASPAERAAMARAFLTACRYELEFFDQARR 527
TenA_C_PH1161-like cd19363
uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA ...
 3-213 8.29e-28

uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes functionally uncharacterized TenA like proteins such as Pyrococcus horikoshii PH1161 protein.


Pssm-ID: 381698 [Multi-domain]  Cd Length: 210  Bit Score: 104.71  E-value: 8.29e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   3 LFSNLIKENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGE 82
Cdd:cd19363     1 ITGKLRRDADDIWKKILNHPFVVELYSGTLPMEKFKFYLLQDYNYLVGSTKNLSILASKAESLDLMRELLELAYGEATTE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  83 MELHRAILS-LGINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIgAEIINRLKNENlKDHPYKE 161
Cdd:cd19363    81 FANYEELLDeLGLSLEDAIKVEPFPTNVAYMNFLLSTSSLGSFYEGLAALLPCFWSYLEI-AEYHKDKLSEN-PNDIYRD 158

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1159373959 162 WILTYGSENFQNEAKEFEDFVNSYTSSvsaQKFQKLSEIFHTVTRLEVAFWE 213
Cdd:cd19363   159 WASVYLSKEYKELVERLRRIVDKYGEG---EPFEKLKRIFKTASKYEYMFWD 207
TenA_C_AtTH2-like cd19368
TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine ...
 9-211 2.41e-25

TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine requiring 2; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Arabidopsis thaliana TH2 is an orphan enzyme thiamin monophosphate phosphatase which has a haloacid dehalogenase (HAD) family domain fused to its TenA_C domain, it's TenA_C domain has thiamin salvage hydrolase activity against amino-HMP. This family includes mostly uncharacterized single-domain TenA_C- like proteins; some however have additional domains such as a HAD family domain or a kinase domain It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381703 [Multi-domain]  Cd Length: 210  Bit Score: 98.08  E-value: 2.41e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   9 KENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYArLAL----NSNTAKELRFAMKFqnyiVEGEME 84
Cdd:cd19368     7 KKNRDEALFALYHPFVVGLAAGNLPLDSFRHYISQDAHFLEAFARAYE-LAEakadDDEDKKAIRELRKG----VLEELK 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  85 LHRAIL-SLGInadelDIKDESLVNIAYSRYM---LSVGeNGDFLDMLVALSA---CAIGYAKIGAEIINRLknENLKDH 157
Cdd:cd19368    82 LHDSYAeEWGV-----DLPKEVTPDPATRKYTdflLATA-SGKVKVAAYTLAAmapCMRLYAFLGQELARAL--DDTEDH 153

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1159373959 158 PYKEWILTYGSENFQNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAF 211
Cdd:cd19368   154 PYKKWIDTYSSQEFEALALQLEDLLDKLSASLTGEELEALEKLYRRAMKLEVEF 207
PTZ00347 PTZ00347
phosphomethylpyrimidine kinase; Provisional
 7-213 7.34e-23

phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 240375 [Multi-domain]  Cd Length: 504  Bit Score: 95.80  E-value: 7.34e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   7 LIKENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLALNSNTAKELRFAMKFQNYIVEGEMELH 86
Cdd:PTZ00347   17 LWKENQDLAMMSLHLPFVQGLGDGTLDQNAFRTYIAQDTLYLNGYIRILSYCITKSDVTATGGGLLELLKGVLEELKNCH 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  87 RAILslginaDELDIKDESLVNIAYSRYMLSVGeNGDFLDMLVALSA---CAIGYAKIGAEIINrlKNENLKDHPYKEWI 163
Cdd:PTZ00347   97 HHYI------DNPDAAGPEAACRKYVDFLLASG-NADTLGPSVVIAAvipCARLYAWVGQELTN--EVELTESHPFRRWL 167

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 1159373959 164 LTYGSENFQNEAKEFEDFVNSYTSSVSaqkFQKLSEIFHTVTRLEVAFWE 213
Cdd:PTZ00347  168 LSYSDEPINTSVEQLESLLDKYIRPGE---FSEVAQAYRRAMELEYDFFD 214
TenA_C_SsTenA-1-like cd19362
uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This ...
 4-216 2.73e-20

uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA like proteins such as Sulfolobus solfataricus putative TenA-like thiaminase (Tena-1, Sso2206).


Pssm-ID: 381697 [Multi-domain]  Cd Length: 200  Bit Score: 84.80  E-value: 2.73e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   4 FSNLIKENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLlnyaKCYARLALNSNTAKELRFAMKFQNYIVEGE- 82
Cdd:cd19362     1 SENLKNLVGDLWNKYVRHEFVERMRDGTLPLDNFRYYLIQDSKYV----EEMLRALLRASSKAPLDKAIKILNSVFSGRd 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  83 --MELHRAILS-LGINADELDIKDESLVNIAYSRYmLSVGENGDFLDMLVALSACAIGYAKIGAEIINRlknenlKDHPY 159
Cdd:cd19362    77 kgMEVHKFLYSeLGITEDEIRRTGYNLVNYAYTRH-LYYYSTLGWPQFLAAWAPCMWGYSEIGKYVLNS------PNELY 149

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1159373959 160 KEWILTYGSENFQNEAKEFEDFVNSYTSSvsaqkfQKLSEIFHTVTRLEVAFWEHSL 216
Cdd:cd19362   150 KTWASFYASKDYKKRVEAILEALDSIEDT------EDIKNIFRNSVNFEIMFWDAAL 200
PRK09517 PRK09517
multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; ...
 22-171 5.16e-16

multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 169939 [Multi-domain]  Cd Length: 755  Bit Score: 76.17  E-value: 5.16e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  22 DFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARLA-LNSNTAKELRFAMKFQNYIVeGEMELHRAILSLGinadeLD 100
Cdd:PRK09517  565 DFIRMLGDGTLRRPEFDFYIDQDAQYLRQYSRALARLSsIAPDSHAQVEWAQSAAECIV-VEAELHRSYLSGK-----EA 638

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1159373959 101 IKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAeiinRLKNENLKDHPYKEWILTYGSENF 171
Cdd:PRK09517  639 PSAPSPVTMAYTDFLIARTYTEDYVVGVAAVLPCYWLYAEIGL----MLAEQNHDEHPYKDWLNTYSGEEF 705
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 7-213 1.13e-15

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 72.60  E-value: 1.13e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   7 LIKENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAK--CYArLALNSNTAKELRFAmKFQNYIVEGEME 84
Cdd:cd19358     5 LRAANAEDWDAAVTHRFVRELCAGTLPDAVLARYLVQDYQFVETFLRllGKA-VAKAPDLEAKLRLA-RFLGFLANDEND 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  85 L-HRAILSLGINADELDIKDESLVNIAYSRYMLSVGENGDFLDMLVALSACAIGYAKIGAEIINRlknenLKDHP-YKEW 162
Cdd:cd19358    83 YfERAFAALGVSEADREAPPLLPATRAFIDLMLEAARSGSYAEILTVLLVAEWLYLDWASRAAAA-----APLRFkHQEW 157

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1159373959 163 ILTYGSENFQNEAKEFEDFVNSYTSSVSAQkfQKLSEIFHTVTRLEVAFWE 213
Cdd:cd19358   158 IDLHSGPEFEAWVDFLRDEVDRVGPTEEER--ERLEAVFARAVELEIAFFD 206
TenA_E_At3g16990-like cd19357
TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins ...
 7-213 2.77e-10

TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Members of this family include Arabidopsis thaliana At3g16990, Zea mays GRMZM2G080501, and Pyrococcus furiosus PF1337, among others. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin; nor does it have activity with other thiamine degradation products such as thiamine mono- or diphosphate, oxythiamine, oxothiamine, thiamine disulfide, desthiothiamine or thiochrome as substrates. Structural studies of P. furiosus PF1337 strongly support its enzymatic function in thiamine biosynthesis. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381692 [Multi-domain]  Cd Length: 217  Bit Score: 57.71  E-value: 2.77e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959   7 LIKENQKIWDAYLHHDFVKKLEDKSLKQENFLFYLKQDYIYLLNYAKCYARL---ALNSNTAKELR----FAMKFQNyiV 79
Cdd:cd19357     4 LLSLHPALYTAATQHPFLRAAADGTLPKEALSRWLAQDRLYVQAYIRFLGSLlarAPLPSSSLNQRlldvLLGALAN--L 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1159373959  80 EGEME-LHRAILSLGINADELDIKdESLVNIAYSRYMLSVGENG-DFLDMLVALSACAIGYAKIGAEIINRLKNENLKDH 157
Cdd:cd19357    82 RRELAfFEETAAEYGLDLPGLGVP-PSPATRAYVDFLASLASEGvSYLEGLVVLWATEKVYLDAWSYARSFLPSDADGGA 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1159373959 158 PYKEWILTYGSENFQNEAKEFEDFVNSYTSSVSAQKFQKLSEIFHTVTRLEVAFWE 213
Cdd:cd19357   161 LYREFIPNWTSPEFAAFVDRLGDLVDEALEQAGEEVLERAEEVWRRVLELEEAFWP 216
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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