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Conserved domains on  [gi|1102824196|ref|WP_071605427|]
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MULTISPECIES: polynucleotide adenylyltransferase PcnB [Enterobacter]

Protein Classification

PcnB family polynucleotide adenylyltransferase( domain architecture ID 11485433)

PcnB family polynucleotide adenylyltransferase, or poly(A) polymerase, creates the 3' poly(A) tail found in some mRNAs

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
pcnB PRK11623
poly(A) polymerase I; Provisional
1-444 0e+00

poly(A) polymerase I; Provisional


:

Pssm-ID: 236939 [Multi-domain]  Cd Length: 472  Bit Score: 941.10  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196   1 MFTRVANFCRKVLSREESMANDAIAQNRMSVIPREQHNISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKD 80
Cdd:PRK11623    8 IFTRVANFCRKVLSREESEAEQAVARPQMTVIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKD 87
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  81 FDVTTSATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEAGESDRTTSQRGQNGMLLRDNIFGSIEEDAQR 160
Cdd:PRK11623   88 FDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNESDRNTSQRGQNGMLLRDNIFGSIEEDAQR 167
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 161 RDFTINSLYYSVADFTVRDYVGGMQDLKDGLIRLIGTPETRYREDPVRMLRAVRFAAKLSMRISPETAEPIPRLATLIND 240
Cdd:PRK11623  168 RDFTINSLYYSVADFTVRDYVGGMKDLKEGVIRLIGNPETRYREDPVRMLRAVRFAAKLDMRISPETAEPIPRLATLLND 247
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 241 VPPARLFEEALKLLQAGYGYETYKLLREYSLFQPLFPTITRCFTENGDSPMERMIAQVLKNTDTRIHNDMRVNPAFLFAA 320
Cdd:PRK11623  248 IPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAA 327
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 321 MFWYPLLEAAQRIAQESGLAYYDAFALAANDVLDEACRTLAIPKRITTLVRDIWQLQLRMSRRQGKRAWKLMEHPKFRAA 400
Cdd:PRK11623  328 MFWYPLLEHAQKIAQESGLAYYDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAA 407
                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....
gi 1102824196 401 FDLLSLRAEIERNQELQRLAQWWGEFQVSAPPEQKDMLVDLDDE 444
Cdd:PRK11623  408 YDLLALRAEVENNAELQRLAQWWGEFQVSAPPQQKGMLNELDDD 451
 
Name Accession Description Interval E-value
pcnB PRK11623
poly(A) polymerase I; Provisional
1-444 0e+00

poly(A) polymerase I; Provisional


Pssm-ID: 236939 [Multi-domain]  Cd Length: 472  Bit Score: 941.10  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196   1 MFTRVANFCRKVLSREESMANDAIAQNRMSVIPREQHNISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKD 80
Cdd:PRK11623    8 IFTRVANFCRKVLSREESEAEQAVARPQMTVIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKD 87
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  81 FDVTTSATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEAGESDRTTSQRGQNGMLLRDNIFGSIEEDAQR 160
Cdd:PRK11623   88 FDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNESDRNTSQRGQNGMLLRDNIFGSIEEDAQR 167
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 161 RDFTINSLYYSVADFTVRDYVGGMQDLKDGLIRLIGTPETRYREDPVRMLRAVRFAAKLSMRISPETAEPIPRLATLIND 240
Cdd:PRK11623  168 RDFTINSLYYSVADFTVRDYVGGMKDLKEGVIRLIGNPETRYREDPVRMLRAVRFAAKLDMRISPETAEPIPRLATLLND 247
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 241 VPPARLFEEALKLLQAGYGYETYKLLREYSLFQPLFPTITRCFTENGDSPMERMIAQVLKNTDTRIHNDMRVNPAFLFAA 320
Cdd:PRK11623  248 IPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAA 327
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 321 MFWYPLLEAAQRIAQESGLAYYDAFALAANDVLDEACRTLAIPKRITTLVRDIWQLQLRMSRRQGKRAWKLMEHPKFRAA 400
Cdd:PRK11623  328 MFWYPLLEHAQKIAQESGLAYYDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAA 407
                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....
gi 1102824196 401 FDLLSLRAEIERNQELQRLAQWWGEFQVSAPPEQKDMLVDLDDE 444
Cdd:PRK11623  408 YDLLALRAEVENNAELQRLAQWWGEFQVSAPPQQKGMLNELDDD 451
pcnB TIGR01942
poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as ...
31-437 2.92e-166

poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as plasmid copy number protein). These enzymes sequentially add adenosine nucleotides to the 3' end of RNAs, targeting them for degradation by the cell. This was originally described for anti-sense RNAs, but was later demonstrated for mRNAs as well. Members of this family are as yet limited to the gamma- and beta-proteobacteria, with putative members in the Chlamydiacae and spirochetes. This family has homology to tRNA nucleotidyltransferase (cca).


Pssm-ID: 130997 [Multi-domain]  Cd Length: 410  Bit Score: 474.67  E-value: 2.92e-166
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  31 VIPREQHNISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTSATPEQVRKLFRNCRLVGRRFRLAH 110
Cdd:TIGR01942   1 VIPESEHNIPRQSFSAHALNVVERLKGAGYQAYIVGGAVRDLLLGIEPKDFDVVTSATPEEVRKLFRNSRIVGRRFRLVH 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 111 VMFGPEIIEVATFRGHHEagesdrttSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLKDG 190
Cdd:TIGR01942  81 VSFGRQIIEVATFRSGHK--------SSVNAEGRILKDNVYGTLEEDAWRRDFTVNALYYDPSREVIIDYVGGMEDLKNR 152
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 191 LIRLIGTPETRYREDPVRMLRAVRFAAKLSMRISPETAEPIPRLATLINDVPPARLFEEALKLLQAGYGYETYKLLREYS 270
Cdd:TIGR01942 153 RLRLIGDPRSRYQEDPVRMLRALRFSVKLEFTIDESTARPIRESAPLLKGIPPARLFEEILKLLFSGRSAALFRMLCGYQ 232
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 271 LFQPLFPTITRCFTENGDSPMERMIAQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLEAAQRIAQEsGLAYYDAFALAAN 350
Cdd:TIGR01942 233 LLEPLFPSVAYALRESPKFESAFTVQALVNDTDFRVKRDKPVTPAFLYAALLWPGLVFRVADAPDQ-GVMPYPAVFDAIR 311
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 351 DVLDEACRTLAIPKRITTLVRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAFDLLSLRAEIERnqELQRLAQWWGEFQVSA 430
Cdd:TIGR01942 312 DFVEEQCAPISIPKRFSIPTREIWQMQLRLERRSGMRARKLLGHARFRAAYDLLLLREVCGR--ENAVLGEWWTPYQLAS 389

                  ....*..
gi 1102824196 431 PPEQKDM 437
Cdd:TIGR01942 390 EEERTEM 396
PcnB COG0617
tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and ...
43-427 1.18e-124

tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and biogenesis]; tRNA nucleotidyltransferase/poly(A) polymerase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440382 [Multi-domain]  Cd Length: 391  Bit Score: 367.99  E-value: 1.18e-124
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  43 DISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTSATPEQVRKLFR---NCRLVGRRFRLAHVMFGPEIIE 119
Cdd:COG0617     1 RLSPNALKVLEALEEAGFEAYLVGGAVRDLLLGRPPKDIDIVTVATPEEVAALFRkalRTVPVGRDFGTVTVVFGGEKIE 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 120 VATFRGHHEAGESDRTtsqrgqngmllrDNIFG-SIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLKDGLIRLIGTP 198
Cdd:COG0617    81 VATARTERYYGDGRRP------------FVEFGdTLEEDLARRDFTINALAYDLNDGELIDPFGGLADLEARVIRTVGDP 148
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 199 ETRYREDPVRMLRAVRFAAKLSMRISPETAEPIPRLATLINDVPPARLFEEALKLLQAGYGYETYKLLREYSLFQPLfpt 278
Cdd:COG0617   149 EERFREDPLRILRAVRFAARLGFTIEPETLAAIREMAGLLDRLSAERVWDELLKLLLSPHPSRGLELLRETGLLEVL--- 225
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 279 itrcftengdspmermiaqvlkntdtrihndmrvnpAFLFAAMFwYPLLEAAQRiaqESGLAYYDAFALAANDVLDEACR 358
Cdd:COG0617   226 ------------------------------------ALRLAALL-HDLGKPATR---EDGLPTFHGHEEAGAELAEALLK 265
                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1102824196 359 TLAIPKRITTLVRDIWQLQLRMSRRQGKRAW---KLMEHpKFRAAFDLLSLRAEIERNQELQ-RLAQW----WGEFQ 427
Cdd:COG0617   266 RLRLPNRERKLVRELVELHLRFHGLGELRDSavrRLLER-GPEALEDLLLLRENGLEYPELQeRLAELleaaWRRFQ 341
PolyA_pol pfam01743
Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, ...
62-193 8.47e-51

Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, such as Poly(A) polymerase, which adds the poly (A) tail to mRNA EC:2.7.7.19. This family also includes the tRNA nucleotidyltransferase that adds the CCA to the 3' of the tRNA EC:2.7.7.25. This family is part of the nucleotidyltransferase superfamily.


Pssm-ID: 396348 [Multi-domain]  Cd Length: 126  Bit Score: 168.23  E-value: 8.47e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  62 AYLVGGGVRDLLLGKKPKDFDVTTSATPEQVRKLFRNCRLV----GRRFRLAHVMFGPEIIEVATFRGHHeaGESDRTTS 137
Cdd:pfam01743   1 LYIVGGAVRDLLLGKTPKDVDIATDATPEQVATLFRRRRIVhllsGIEFGTIHVIFGNQILEVATFRIEF--DESDFRNP 78
                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1102824196 138 QRGQNGmllrdnifGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLKDGLIR 193
Cdd:pfam01743  79 RSEEYT--------GTLEEDAKRRDFTINALAYNPNSGEVIDYFGGIKDLKSGVIR 126
NT_ClassII-CCAase cd05398
Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the ...
45-189 3.15e-43

Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This Class II group is comprised mainly of eubacterial and eukaryotic enzymes and includes Bacillus stearothermophilus CCAase, Escherichia coli poly(A) polymerase I, human mitochondrial CCAase, and Saccharomyces cerevisiae CCAase (CCA1). CCA-adding enzymes have a single catalytic pocket, which recognizes both ATP and CTP substrates. Included in this subgroup are CC- and A-adding enzymes from various ancient species of bacteria such as Aquifex aeolicus; these enzymes collaborate to add CCA to tRNAs. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family. Escherichia coli CCAase is related to this group but has not been included in this alignment as this enzyme lacks the N-terminal helix conserved in the remainder of the NT superfamily.


Pssm-ID: 143388 [Multi-domain]  Cd Length: 139  Bit Score: 148.90  E-value: 3.15e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  45 SENALKVLYRLNKA-GYEAYLVGGGVRDLLLGKKPKDFDVTTSA-TPEQVRKLFR--NCRLVGR--RFRLAHVMFGPEII 118
Cdd:cd05398     1 TPELLKLLRELKKAlGYEAYLVGGAVRDLLLGRPPKDIDIATDAdGPEFAEALFKkiGGRVVGLgeEFGTATVVINGLTI 80
                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1102824196 119 EVATFRGHHEAgesdrttsqrgQNGMLLRDNIFGsIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLKD 189
Cdd:cd05398    81 DVATLRTETYT-----------DPGRRPPVVGFT-IEEDLLRRDFTINAMAYDLDDGELIDPFGGLKDLEN 139
 
Name Accession Description Interval E-value
pcnB PRK11623
poly(A) polymerase I; Provisional
1-444 0e+00

poly(A) polymerase I; Provisional


Pssm-ID: 236939 [Multi-domain]  Cd Length: 472  Bit Score: 941.10  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196   1 MFTRVANFCRKVLSREESMANDAIAQNRMSVIPREQHNISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKD 80
Cdd:PRK11623    8 IFTRVANFCRKVLSREESEAEQAVARPQMTVIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKD 87
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  81 FDVTTSATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEAGESDRTTSQRGQNGMLLRDNIFGSIEEDAQR 160
Cdd:PRK11623   88 FDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNESDRNTSQRGQNGMLLRDNIFGSIEEDAQR 167
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 161 RDFTINSLYYSVADFTVRDYVGGMQDLKDGLIRLIGTPETRYREDPVRMLRAVRFAAKLSMRISPETAEPIPRLATLIND 240
Cdd:PRK11623  168 RDFTINSLYYSVADFTVRDYVGGMKDLKEGVIRLIGNPETRYREDPVRMLRAVRFAAKLDMRISPETAEPIPRLATLLND 247
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 241 VPPARLFEEALKLLQAGYGYETYKLLREYSLFQPLFPTITRCFTENGDSPMERMIAQVLKNTDTRIHNDMRVNPAFLFAA 320
Cdd:PRK11623  248 IPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAA 327
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 321 MFWYPLLEAAQRIAQESGLAYYDAFALAANDVLDEACRTLAIPKRITTLVRDIWQLQLRMSRRQGKRAWKLMEHPKFRAA 400
Cdd:PRK11623  328 MFWYPLLEHAQKIAQESGLAYYDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAA 407
                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....
gi 1102824196 401 FDLLSLRAEIERNQELQRLAQWWGEFQVSAPPEQKDMLVDLDDE 444
Cdd:PRK11623  408 YDLLALRAEVENNAELQRLAQWWGEFQVSAPPQQKGMLNELDDD 451
pcnB TIGR01942
poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as ...
31-437 2.92e-166

poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as plasmid copy number protein). These enzymes sequentially add adenosine nucleotides to the 3' end of RNAs, targeting them for degradation by the cell. This was originally described for anti-sense RNAs, but was later demonstrated for mRNAs as well. Members of this family are as yet limited to the gamma- and beta-proteobacteria, with putative members in the Chlamydiacae and spirochetes. This family has homology to tRNA nucleotidyltransferase (cca).


Pssm-ID: 130997 [Multi-domain]  Cd Length: 410  Bit Score: 474.67  E-value: 2.92e-166
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  31 VIPREQHNISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTSATPEQVRKLFRNCRLVGRRFRLAH 110
Cdd:TIGR01942   1 VIPESEHNIPRQSFSAHALNVVERLKGAGYQAYIVGGAVRDLLLGIEPKDFDVVTSATPEEVRKLFRNSRIVGRRFRLVH 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 111 VMFGPEIIEVATFRGHHEagesdrttSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLKDG 190
Cdd:TIGR01942  81 VSFGRQIIEVATFRSGHK--------SSVNAEGRILKDNVYGTLEEDAWRRDFTVNALYYDPSREVIIDYVGGMEDLKNR 152
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 191 LIRLIGTPETRYREDPVRMLRAVRFAAKLSMRISPETAEPIPRLATLINDVPPARLFEEALKLLQAGYGYETYKLLREYS 270
Cdd:TIGR01942 153 RLRLIGDPRSRYQEDPVRMLRALRFSVKLEFTIDESTARPIRESAPLLKGIPPARLFEEILKLLFSGRSAALFRMLCGYQ 232
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 271 LFQPLFPTITRCFTENGDSPMERMIAQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLEAAQRIAQEsGLAYYDAFALAAN 350
Cdd:TIGR01942 233 LLEPLFPSVAYALRESPKFESAFTVQALVNDTDFRVKRDKPVTPAFLYAALLWPGLVFRVADAPDQ-GVMPYPAVFDAIR 311
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 351 DVLDEACRTLAIPKRITTLVRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAFDLLSLRAEIERnqELQRLAQWWGEFQVSA 430
Cdd:TIGR01942 312 DFVEEQCAPISIPKRFSIPTREIWQMQLRLERRSGMRARKLLGHARFRAAYDLLLLREVCGR--ENAVLGEWWTPYQLAS 389

                  ....*..
gi 1102824196 431 PPEQKDM 437
Cdd:TIGR01942 390 EEERTEM 396
PcnB COG0617
tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and ...
43-427 1.18e-124

tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and biogenesis]; tRNA nucleotidyltransferase/poly(A) polymerase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440382 [Multi-domain]  Cd Length: 391  Bit Score: 367.99  E-value: 1.18e-124
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  43 DISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTSATPEQVRKLFR---NCRLVGRRFRLAHVMFGPEIIE 119
Cdd:COG0617     1 RLSPNALKVLEALEEAGFEAYLVGGAVRDLLLGRPPKDIDIVTVATPEEVAALFRkalRTVPVGRDFGTVTVVFGGEKIE 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 120 VATFRGHHEAGESDRTtsqrgqngmllrDNIFG-SIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLKDGLIRLIGTP 198
Cdd:COG0617    81 VATARTERYYGDGRRP------------FVEFGdTLEEDLARRDFTINALAYDLNDGELIDPFGGLADLEARVIRTVGDP 148
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 199 ETRYREDPVRMLRAVRFAAKLSMRISPETAEPIPRLATLINDVPPARLFEEALKLLQAGYGYETYKLLREYSLFQPLfpt 278
Cdd:COG0617   149 EERFREDPLRILRAVRFAARLGFTIEPETLAAIREMAGLLDRLSAERVWDELLKLLLSPHPSRGLELLRETGLLEVL--- 225
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 279 itrcftengdspmermiaqvlkntdtrihndmrvnpAFLFAAMFwYPLLEAAQRiaqESGLAYYDAFALAANDVLDEACR 358
Cdd:COG0617   226 ------------------------------------ALRLAALL-HDLGKPATR---EDGLPTFHGHEEAGAELAEALLK 265
                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1102824196 359 TLAIPKRITTLVRDIWQLQLRMSRRQGKRAW---KLMEHpKFRAAFDLLSLRAEIERNQELQ-RLAQW----WGEFQ 427
Cdd:COG0617   266 RLRLPNRERKLVRELVELHLRFHGLGELRDSavrRLLER-GPEALEDLLLLRENGLEYPELQeRLAELleaaWRRFQ 341
PRK13299 PRK13299
tRNA CCA-pyrophosphorylase; Provisional
42-273 4.62e-52

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 237339 [Multi-domain]  Cd Length: 394  Bit Score: 180.42  E-value: 4.62e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  42 KDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTSATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVA 121
Cdd:PRK13299    3 PSEFQKALPILEKIKEAGFEAYFVGGSVRDYLLGRPIHDVDIATSAYPEEVKAIFPRTVDVGIEHGTVLVLENGEEYEVT 82
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 122 TFRGhheagESDRTTSQRGQNGMLLRdnifgSIEEDAQRRDFTINSLYYSvADFTVRDYVGGMQDLKDGLIRLIGTPETR 201
Cdd:PRK13299   83 TFRT-----ESEYVDYRRPSEVTFVR-----SLEEDLKRRDFTINAIAMD-ENGEIIDLFDGLEDLKNRLIRAVGNAEER 151
                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1102824196 202 YREDPVRMLRAVRFAAKLSMRISPETAEPIPRLATLINDVPPARLFEEALKLLQAGYGYETYKLLREYSLFQ 273
Cdd:PRK13299  152 FQEDALRMMRAVRFASQLGFDLETETFEAMKTQAPLLEKISVERIFVEFEKLLLGPFWRKGLKLLIETGLYN 223
PolyA_pol pfam01743
Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, ...
62-193 8.47e-51

Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, such as Poly(A) polymerase, which adds the poly (A) tail to mRNA EC:2.7.7.19. This family also includes the tRNA nucleotidyltransferase that adds the CCA to the 3' of the tRNA EC:2.7.7.25. This family is part of the nucleotidyltransferase superfamily.


Pssm-ID: 396348 [Multi-domain]  Cd Length: 126  Bit Score: 168.23  E-value: 8.47e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  62 AYLVGGGVRDLLLGKKPKDFDVTTSATPEQVRKLFRNCRLV----GRRFRLAHVMFGPEIIEVATFRGHHeaGESDRTTS 137
Cdd:pfam01743   1 LYIVGGAVRDLLLGKTPKDVDIATDATPEQVATLFRRRRIVhllsGIEFGTIHVIFGNQILEVATFRIEF--DESDFRNP 78
                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1102824196 138 QRGQNGmllrdnifGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLKDGLIR 193
Cdd:pfam01743  79 RSEEYT--------GTLEEDAKRRDFTINALAYNPNSGEVIDYFGGIKDLKSGVIR 126
PolyA_pol_arg_C pfam12626
Polymerase A arginine-rich C-terminus; The C-terminus of polymerase A in E coli is ...
337-444 4.03e-45

Polymerase A arginine-rich C-terminus; The C-terminus of polymerase A in E coli is arginine-rich and is necessary for full functioning of the enzyme.


Pssm-ID: 463647 [Multi-domain]  Cd Length: 116  Bit Score: 153.13  E-value: 4.03e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 337 SGLAYYDAFALAANDVLDEACRTLAIPKRITTLVRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAFDLLSLRAEIErnQEL 416
Cdd:pfam12626   1 QGLPPYDALQLAADEVLDEQCQRVAIPRRFTAPMREIWLLQPRLEKRQGKRPFRLLEHPRFRAAYDFLLLRAESG--EVL 78
                          90       100
                  ....*....|....*....|....*...
gi 1102824196 417 QRLAQWWGEFQVSAPPEQKDMLVDLDDE 444
Cdd:pfam12626  79 QELAEWWTRFQEADEAEREAMIAELGAD 106
NT_ClassII-CCAase cd05398
Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the ...
45-189 3.15e-43

Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This Class II group is comprised mainly of eubacterial and eukaryotic enzymes and includes Bacillus stearothermophilus CCAase, Escherichia coli poly(A) polymerase I, human mitochondrial CCAase, and Saccharomyces cerevisiae CCAase (CCA1). CCA-adding enzymes have a single catalytic pocket, which recognizes both ATP and CTP substrates. Included in this subgroup are CC- and A-adding enzymes from various ancient species of bacteria such as Aquifex aeolicus; these enzymes collaborate to add CCA to tRNAs. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family. Escherichia coli CCAase is related to this group but has not been included in this alignment as this enzyme lacks the N-terminal helix conserved in the remainder of the NT superfamily.


Pssm-ID: 143388 [Multi-domain]  Cd Length: 139  Bit Score: 148.90  E-value: 3.15e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  45 SENALKVLYRLNKA-GYEAYLVGGGVRDLLLGKKPKDFDVTTSA-TPEQVRKLFR--NCRLVGR--RFRLAHVMFGPEII 118
Cdd:cd05398     1 TPELLKLLRELKKAlGYEAYLVGGAVRDLLLGRPPKDIDIATDAdGPEFAEALFKkiGGRVVGLgeEFGTATVVINGLTI 80
                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1102824196 119 EVATFRGHHEAgesdrttsqrgQNGMLLRDNIFGsIEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLKD 189
Cdd:cd05398    81 DVATLRTETYT-----------DPGRRPPVVGFT-IEEDLLRRDFTINAMAYDLDDGELIDPFGGLKDLEN 139
PolyA_pol_RNAbd pfam12627
Probable RNA and SrmB- binding site of polymerase A; This region encompasses much of the RNA ...
221-277 6.76e-18

Probable RNA and SrmB- binding site of polymerase A; This region encompasses much of the RNA and SrmB binding motifs on polymerase A.


Pssm-ID: 463648 [Multi-domain]  Cd Length: 64  Bit Score: 77.52  E-value: 6.76e-18
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1102824196 221 MRISPETAEPIPRLATLINDVPPARLFEEALKLLQAGYGYETYKLLREYSLFQPLFP 277
Cdd:pfam12627   2 FTIEPETREAIRKLAPLLKKISPERIFEELLKLLLSGHPERGLELLRETGLLEYLFP 58
cca PRK10885
multifunctional CCA addition/repair protein;
63-229 2.11e-16

multifunctional CCA addition/repair protein;


Pssm-ID: 182810 [Multi-domain]  Cd Length: 409  Bit Score: 80.67  E-value: 2.11e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  63 YLVGGGVRDLLLGKKPKDFD-VTTSATPEQVrkLFRNCRLVGRRFRlahVMFGPEIievatfrghHEAGESDRT--TSQR 139
Cdd:PRK10885    4 YLVGGAVRDALLGLPVKDRDwVVVGATPEEM--LAQGYQQVGKDFP---VFLHPKT---------HEEYALARTerKSGR 69
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 140 GQNGMllrdnIFG-----SIEEDAQRRDFTINSLYYSvADFTVRDYVGGMQDLKDGLIRLIGTPetrYREDPVRMLRAVR 214
Cdd:PRK10885   70 GYTGF-----TCYaapdvTLEEDLIRRDLTINAMAQD-DDGELIDPYGGQRDLEARLLRHVSPA---FAEDPLRVLRVAR 140
                         170
                  ....*....|....*...
gi 1102824196 215 FAAK---LSMRISPETAE 229
Cdd:PRK10885  141 FAARfahLGFRIAPETLA 158
PRK13297 PRK13297
tRNA CCA-pyrophosphorylase; Provisional
58-256 5.82e-15

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 139469 [Multi-domain]  Cd Length: 364  Bit Score: 76.19  E-value: 5.82e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  58 AGYEAYLVGGGVRDLLLGKKPKDFD-VTTSATPEQVRKlfRNCRLVGRRFRlahVMFGPEIIEvatfrgHHEAGESDRtT 136
Cdd:PRK13297   10 AGLQVYIVGGAVRDALLGLPAGDRDwVVVGATPEDMAR--RGFIPVGGDFP---VFLHPRTKE------EYALARTER-K 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 137 SQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVrDYVGGMQDLKDGLIRLIGTPetrYREDPVRMLRAVRFA 216
Cdd:PRK13297   78 SGRGYKGFTFYTGADVTLEQDLQRRDLTVNAIARTPQGELV-DPLDGVADVRARVLRHVGEA---FAEDPVRILRLGRFA 153
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|...
gi 1102824196 217 AKLS-MRISPETAEPIPRL--ATLINDVPPARLFEEALKLLQA 256
Cdd:PRK13297  154 ARFGdFSIAPETMQLCRRMveAGEADALVPERVWKEVSRGLMA 196
PRK13298 PRK13298
tRNA CCA-pyrophosphorylase; Provisional
61-227 1.15e-14

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 237338 [Multi-domain]  Cd Length: 417  Bit Score: 75.54  E-value: 1.15e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  61 EAYLVGGGVRDLLLGKKPKDFD-VTTSATPEQVrkLFRNCRLVGRRFRlahVMFGPEiievatfrgHHEAGESDRT--TS 137
Cdd:PRK13298    2 KIYLVGGAVRDSLLNLPVKDKDwVVVGGTPKIL--LSINFQQVGKDFP---VFLHPE---------THEEYALARTerKS 67
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 138 QRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSvADFTVRDYVGGMQDLKDGLIRLIGTPetrYREDPVRMLRAVRFAA 217
Cdd:PRK13298   68 GVGYTGFITDTSSDVTLEEDLIRRDLTINAIAQD-ENGNYIDPFQGKKDIQLRLLRHVSES---FIEDPLRVLRVARFAA 143
                         170
                  ....*....|...
gi 1102824196 218 KLS---MRISPET 227
Cdd:PRK13298  144 LLVhlgFKIAKET 156
PRK13296 PRK13296
CCA tRNA nucleotidyltransferase;
61-311 1.25e-14

CCA tRNA nucleotidyltransferase;


Pssm-ID: 106256 [Multi-domain]  Cd Length: 360  Bit Score: 75.02  E-value: 1.25e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  61 EAYLVGGGVRDLLLGKKPKDFD-VTTSATPEQVRKlfrncrlVGRRFRLAH--VMFGPEIIEvatfrgHHEAGESDRTTS 137
Cdd:PRK13296    2 KFYLVGGAVRDMLLGITPKDKDwVVVGATEDEMLA-------NGFIKIAANfpVFIHPQTKQ------EYALARSEKKTA 68
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 138 qRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSvADFTVRDYVGGMQDLKDGLIRliGTPETrYREDPVRMLRAVRFAA 217
Cdd:PRK13296   69 -SGYHGFEVNFSKYITLEDDLKRRDLTINSIAID-QNNKVIDPFNGQADLQNRILR--HTSIA-FIEDPLRVVRLARFKA 143
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 218 KLS---MRISPETAEPIPRLATL--INDVPPARLFEEALKLLQAGYGYetYKLLREYSLFQPLFPTIT--------RCFT 284
Cdd:PRK13296  144 QLSnfnFSIAQEMLALIKELVKTgeLNHLTRERLHIEFVKALNNPKIF--FTTLKELEALKIIFPNIScilplipnKSFF 221
                         250       260       270
                  ....*....|....*....|....*....|
gi 1102824196 285 ENG---DSPMERMIAQVLKNTDTRIHNDMR 311
Cdd:PRK13296  222 ENPiykGSNINEKITLCLLKIPQQQLDDIR 251
PHA01806 PHA01806
hypothetical protein
29-218 2.96e-06

hypothetical protein


Pssm-ID: 222838  Cd Length: 200  Bit Score: 47.95  E-value: 2.96e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196  29 MSVIPREQHnISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTT-SATPEQVRKLFrNCRLvgrrfr 107
Cdd:PHA01806    6 SDLLEGTKE-IPEGLIAKALLLRLYSDARHSEGVALAGGAARDLMHGAEPKDIDIALyGMDDRQAELLI-GCIL------ 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1102824196 108 lahVMFGPEIIEVATFRG-HHEAGESDRTTSQ------RGQNGMLLRDNIF--GSIEEDAQRRDFTINSLyysVADFTVR 178
Cdd:PHA01806   78 ---PKLVSRFLRDGGWSQeYHDGGDGGIFKGVvslkgcRGLEGMDVDFNYYdtDSVGEVMESFDYTINQV---GIAYNWP 151
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1102824196 179 DYVGG-------MQDLKDGLIRligtpetRYREDPVRMLRAVRFAAK 218
Cdd:PHA01806  152 DPEGGprlgaylHKDVTWGVNK-------ELRAGSRLPERAEKMRAK 191
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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