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Conserved domains on  [gi|1005571659|ref|WP_061664900|]
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MULTISPECIES: stage IV sporulation protein SpoIVFA [Bacillus]

Protein Classification

M23 family metallopeptidase( domain architecture ID 10480195)

metallopeptidase of the M23 family, that include a variety of zinc metallo-endopeptidases with a range of specificities, not all members are active enzymes

CATH:  2.70.70.10
EC:  3.4.-.-
Gene Ontology:  GO:0046872|GO:0006508
MEROPS:  M23
SCOP:  4000931

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SpoIVFA COG5833
Stage IV sporulation protein SpoIVFA, regulates SpoIVFB [Cell cycle control, cell division, ...
 49-248 3.58e-95

Stage IV sporulation protein SpoIVFA, regulates SpoIVFB [Cell cycle control, cell division, chromosome partitioning];


:

Pssm-ID: 444535 [Multi-domain]  Cd Length: 219  Bit Score: 278.03  E-value: 3.58e-95
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659  49 HPLFRKEVFFFKVLLSAILVLSVAILYKNAPSSFNGAKAVTEKVMKEEFQFATVVKWYEKQFGKPLVFYSPNEKKEGSIQ 128
Cdd:COG5833    20 HPLKRSSKFLVKVLISACLFLLVAILFQSDSPPLEKAKAWVATVLTEEFQFAAVNQWYEETFGGPLAFLPPFGKEEETVE 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 129 QK-DYAIPASGKVMQGFQKNGQGVFVQTATNAKVESVNEGLVVFAGKKEELGNTVQIQHADGTESWYANLNDMSVKLYDY 207
Cdd:COG5833   100 QGeAFALPVSGKVVESFQENGKGVDIETPGGANVKAVKEGYVIFAGKDEETGKTVIIQHADGSESWYGNLSSIDVKLYDF 179

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 1005571659 208 VSKKQKIGTVSNdTNDKNGKFYFAIKKNEKFIDPIQVISFD 248
Cdd:COG5833   180 VEAGQKIGTVPA-TEGEEGTFYFAIKKGGKFIDPIQVISFD 219
 
Name Accession Description Interval E-value
SpoIVFA COG5833
Stage IV sporulation protein SpoIVFA, regulates SpoIVFB [Cell cycle control, cell division, ...
 49-248 3.58e-95

Stage IV sporulation protein SpoIVFA, regulates SpoIVFB [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 444535 [Multi-domain]  Cd Length: 219  Bit Score: 278.03  E-value: 3.58e-95
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659  49 HPLFRKEVFFFKVLLSAILVLSVAILYKNAPSSFNGAKAVTEKVMKEEFQFATVVKWYEKQFGKPLVFYSPNEKKEGSIQ 128
Cdd:COG5833    20 HPLKRSSKFLVKVLISACLFLLVAILFQSDSPPLEKAKAWVATVLTEEFQFAAVNQWYEETFGGPLAFLPPFGKEEETVE 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 129 QK-DYAIPASGKVMQGFQKNGQGVFVQTATNAKVESVNEGLVVFAGKKEELGNTVQIQHADGTESWYANLNDMSVKLYDY 207
Cdd:COG5833   100 QGeAFALPVSGKVVESFQENGKGVDIETPGGANVKAVKEGYVIFAGKDEETGKTVIIQHADGSESWYGNLSSIDVKLYDF 179

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 1005571659 208 VSKKQKIGTVSNdTNDKNGKFYFAIKKNEKFIDPIQVISFD 248
Cdd:COG5833   180 VEAGQKIGTVPA-TEGEEGTFYFAIKKGGKFIDPIQVISFD 219
Peptidase_M23 pfam01551
Peptidase family M23; Members of this family are zinc metallopeptidases with a range of ...
 149-241 3.46e-24

Peptidase family M23; Members of this family are zinc metallopeptidases with a range of specificities. The peptidase family M23 is included in this family, these are Gly-Gly endopeptidases. Peptidase family M23 are also endopeptidases. This family also includes some bacterial lipoproteins such as Swiss:P33648 for which no proteolytic activity has been demonstrated. This family also includes leukocyte cell-derived chemotaxin 2 (LECT2) proteins. LECT2 is a liver-specific protein which is thought to be linked to hepatocyte growth although the exact function of this protein is unknown.


Pssm-ID: 460250 [Multi-domain]  Cd Length: 96  Bit Score: 92.61  E-value: 3.46e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 149 QGVFVQTATNAKVESVNEGLVVFAGKKEELGNTVQIQHADGTESWYANLNDMSVKLYDYVSKKQKIGTVSNDTNDKNGKF 228
Cdd:pfam01551   4 KGIDIAAPTGTPVYAAADGVVVFAGWLGGYGNLVIIDHGNGYSTLYAHLSSILVKVGQRVKAGQVIGTVGSTGRSTGPHL 83

                          90
                  ....*....|...
gi 1005571659 229 YFAIKKNEKFIDP 241
Cdd:pfam01551  84 HFEIRKNGKPVDP 96
M23_peptidase cd12797
M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins; ...
 149-232 3.82e-17

M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins; This model describes the metallopeptidase M23 family, which includes beta-lytic metallopeptidase and lysostaphin. Members of this family are zinc endopeptidases that lyse bacterial cell wall peptidoglycans; they cleave either the N-acylmuramoyl-Ala bond between the cell wall peptidoglycan and the cross-linking peptide (e.g. beta-lytic endopeptidase) or a bond within the cross-linking peptide (e.g. stapholysin, and lysostaphin). Beta-lytic metallopeptidase, formerly known as beta-lytic protease, has a preference for cleavage of Gly-X bonds and favors hydrophobic or apolar residues on either side. It inhibits growth of sensitive organisms and may potentially serve as an antimicrobial agent. Lysostaphin, produced by Staphylococcus genus, cleaves pentaglycine cross-bridges of cell wall peptidoglycan, acting as autolysins to maintain cell wall metabolism or as toxins and weapons against competing strains. Staphylolysin (also known as LasA) is implicated in a range of processes related to Pseudomonas virulence, including stimulating shedding of the ectodomain of cell surface heparan sulphate proteoglycan syndecan-1, and elastin degradation in connective tissue. Its active site is less constricted and contains a five-coordinate zinc ion with trigonal bipyramidal geometry and two metal-bound water molecules, possibly contributing to its activity against a wider range of substrates than those used by related lytic enzymes, consistent with its multiple roles in Pseudomonas virulence. The family includes members that do not appear to have the conserved zinc-binding site and might be lipoproteins lacking proteolytic activity.


Pssm-ID: 410984 [Multi-domain]  Cd Length: 85  Bit Score: 73.78  E-value: 3.82e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 149 QGVFVQTATNAKVESVNEGLVVFAGKKEELGNTVQIQHADGTESWYANLNDMSVKLYDYVSKKQKIGTVSNDTNDKNGKF 228
Cdd:cd12797     2 NGIDIAAPEGTPVYAAADGTVVFAGWDGGYGNYVIIDHGNGYYTLYAHLSSILVKVGQRVKKGQVIGTVGNTGRSTGPHL 81


                  ....
gi 1005571659 229 YFAI 232
Cdd:cd12797    82 HFEI 85
nlpD PRK10871
murein hydrolase activator NlpD;
135-243 3.43e-04

murein hydrolase activator NlpD;


Pssm-ID: 236782 [Multi-domain]  Cd Length: 319  Bit Score: 40.97  E-value: 3.43e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 135 PASGKVMQGF---QKNGQGVFVQTATNAKVESVNEGLVVFAGKK-EELGNTVQIQHADGTESWYANLNDMSVKLYDYVSK 210
Cdd:PRK10871  203 PTDGKVIENFsasEGGNKGIDIAGSKGQAIIATADGRVVYAGNAlRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKA 282

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1005571659 211 KQKIGTVSNdTNDKNGKFYFAIKKNEKFIDPIQ 243
Cdd:PRK10871  283 GQKIATMGS-TGTSSTRLHFEIRYKGKSVNPLR 314
 
Name Accession Description Interval E-value
SpoIVFA COG5833
Stage IV sporulation protein SpoIVFA, regulates SpoIVFB [Cell cycle control, cell division, ...
 49-248 3.58e-95

Stage IV sporulation protein SpoIVFA, regulates SpoIVFB [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 444535 [Multi-domain]  Cd Length: 219  Bit Score: 278.03  E-value: 3.58e-95
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659  49 HPLFRKEVFFFKVLLSAILVLSVAILYKNAPSSFNGAKAVTEKVMKEEFQFATVVKWYEKQFGKPLVFYSPNEKKEGSIQ 128
Cdd:COG5833    20 HPLKRSSKFLVKVLISACLFLLVAILFQSDSPPLEKAKAWVATVLTEEFQFAAVNQWYEETFGGPLAFLPPFGKEEETVE 99

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 129 QK-DYAIPASGKVMQGFQKNGQGVFVQTATNAKVESVNEGLVVFAGKKEELGNTVQIQHADGTESWYANLNDMSVKLYDY 207
Cdd:COG5833   100 QGeAFALPVSGKVVESFQENGKGVDIETPGGANVKAVKEGYVIFAGKDEETGKTVIIQHADGSESWYGNLSSIDVKLYDF 179

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 1005571659 208 VSKKQKIGTVSNdTNDKNGKFYFAIKKNEKFIDPIQVISFD 248
Cdd:COG5833   180 VEAGQKIGTVPA-TEGEEGTFYFAIKKGGKFIDPIQVISFD 219
Peptidase_M23 pfam01551
Peptidase family M23; Members of this family are zinc metallopeptidases with a range of ...
 149-241 3.46e-24

Peptidase family M23; Members of this family are zinc metallopeptidases with a range of specificities. The peptidase family M23 is included in this family, these are Gly-Gly endopeptidases. Peptidase family M23 are also endopeptidases. This family also includes some bacterial lipoproteins such as Swiss:P33648 for which no proteolytic activity has been demonstrated. This family also includes leukocyte cell-derived chemotaxin 2 (LECT2) proteins. LECT2 is a liver-specific protein which is thought to be linked to hepatocyte growth although the exact function of this protein is unknown.


Pssm-ID: 460250 [Multi-domain]  Cd Length: 96  Bit Score: 92.61  E-value: 3.46e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 149 QGVFVQTATNAKVESVNEGLVVFAGKKEELGNTVQIQHADGTESWYANLNDMSVKLYDYVSKKQKIGTVSNDTNDKNGKF 228
Cdd:pfam01551   4 KGIDIAAPTGTPVYAAADGVVVFAGWLGGYGNLVIIDHGNGYSTLYAHLSSILVKVGQRVKAGQVIGTVGSTGRSTGPHL 83

                          90
                  ....*....|...
gi 1005571659 229 YFAIKKNEKFIDP 241
Cdd:pfam01551  84 HFEIRKNGKPVDP 96
EnvC COG4942
Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, ...
 135-246 5.21e-24

Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 443969 [Multi-domain]  Cd Length: 377  Bit Score: 98.68  E-value: 5.21e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 135 PASGKVMQGF------QKNGQGVFVQTATNAKVESVNEGLVVFAGKKEELGNTVQIQHADGTESWYANLNDMSVKLYDYV 208
Cdd:COG4942   258 PVSGRVVRRFgerdggGGRNKGIDIAAPPGAPVRAVADGTVVYAGWLRGYGNLVIIDHGGGYLTLYAHLSSLLVKVGQRV 337

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 1005571659 209 SKKQKIGTVSNDTNDKNGKFYFAIKKNEKFIDPIQVIS 246
Cdd:COG4942   338 KAGQPIGTVGSSGGQGGPTLYFELRKNGKPVDPLPWLA 375
M23_peptidase cd12797
M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins; ...
 149-232 3.82e-17

M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins; This model describes the metallopeptidase M23 family, which includes beta-lytic metallopeptidase and lysostaphin. Members of this family are zinc endopeptidases that lyse bacterial cell wall peptidoglycans; they cleave either the N-acylmuramoyl-Ala bond between the cell wall peptidoglycan and the cross-linking peptide (e.g. beta-lytic endopeptidase) or a bond within the cross-linking peptide (e.g. stapholysin, and lysostaphin). Beta-lytic metallopeptidase, formerly known as beta-lytic protease, has a preference for cleavage of Gly-X bonds and favors hydrophobic or apolar residues on either side. It inhibits growth of sensitive organisms and may potentially serve as an antimicrobial agent. Lysostaphin, produced by Staphylococcus genus, cleaves pentaglycine cross-bridges of cell wall peptidoglycan, acting as autolysins to maintain cell wall metabolism or as toxins and weapons against competing strains. Staphylolysin (also known as LasA) is implicated in a range of processes related to Pseudomonas virulence, including stimulating shedding of the ectodomain of cell surface heparan sulphate proteoglycan syndecan-1, and elastin degradation in connective tissue. Its active site is less constricted and contains a five-coordinate zinc ion with trigonal bipyramidal geometry and two metal-bound water molecules, possibly contributing to its activity against a wider range of substrates than those used by related lytic enzymes, consistent with its multiple roles in Pseudomonas virulence. The family includes members that do not appear to have the conserved zinc-binding site and might be lipoproteins lacking proteolytic activity.


Pssm-ID: 410984 [Multi-domain]  Cd Length: 85  Bit Score: 73.78  E-value: 3.82e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 149 QGVFVQTATNAKVESVNEGLVVFAGKKEELGNTVQIQHADGTESWYANLNDMSVKLYDYVSKKQKIGTVSNDTNDKNGKF 228
Cdd:cd12797     2 NGIDIAAPEGTPVYAAADGTVVFAGWDGGYGNYVIIDHGNGYYTLYAHLSSILVKVGQRVKKGQVIGTVGNTGRSTGPHL 81


                  ....
gi 1005571659 229 YFAI 232
Cdd:cd12797    82 HFEI 85
SpoIIQ2 COG5821
Stage II sporulation protein SpoIIQ, clostridial version, metallopeptidase M23 family [Cell ...
 51-247 2.30e-16

Stage II sporulation protein SpoIIQ, clostridial version, metallopeptidase M23 family [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 444523 [Multi-domain]  Cd Length: 200  Bit Score: 74.68  E-value: 2.30e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659  51 LFRKEVFFFKVLLSAILVLSVAILYKNapSSFNGAKAVTEKVMKEEFqfatvvkwyekqfgkplvfySPNEKKEGSIQQK 130
Cdd:COG5821    10 FLDKKGFYIVLFLCICIVAATAVWVTN--NNLNKLEEETVKNKSESN--------------------EKSKSKVTASTSN 67

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 131 DYAIPASGKVMQGFQKNG------------QGVFVQTATNAKVESVNEGLVVFAGKKEELGNTVQIQHADGTESWYANLN 198
Cdd:COG5821    68 KFLKPVSGKITREFGEDLvysktlnewrthTGIDIAAKEGTPVKAAADGVVVEVGKDPKYGITVVIDHGNGIKTVYANLD 147

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1005571659 199 D-MSVKLYDYVSKKQKIGTVSNDTNDKNGK---FYFAIKKNEKFIDPIQVISF 247
Cdd:COG5821   148 SkIKVKVGQKVKKGQVIGKVGSTALFESSEgphLHFEVLKNGKPVDPMKYLKK 200
NlpD COG0739
Murein DD-endopeptidase MepM and murein hydrolase activator NlpD, contains LysM domain [Cell ...
 135-245 3.94e-15

Murein DD-endopeptidase MepM and murein hydrolase activator NlpD, contains LysM domain [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440502 [Multi-domain]  Cd Length: 196  Bit Score: 71.54  E-value: 3.94e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 135 PASGKVMQGFQKNGQGVFVQT----------ATNAKVESVNEGLVVFAGKKEELGNTVQIQHADGTESWYANLNDMSVKL 204
Cdd:COG0739    74 PVKGRITSGFGYRRHPVTGRRrfhkgidiaaPTGTPVYAAADGTVVFAGWNGGYGNLVIIDHGNGYTTLYAHLSSILVKV 153

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1005571659 205 YDYVSKKQKIGTVSNDTNDKNGKFYFAIKKNEKFIDPIQVI 245
Cdd:COG0739   154 GQRVKAGQVIGYVGNTGRSTGPHLHFEVRVNGKPVDPLPFL 194
nlpD PRK10871
murein hydrolase activator NlpD;
135-243 3.43e-04

murein hydrolase activator NlpD;


Pssm-ID: 236782 [Multi-domain]  Cd Length: 319  Bit Score: 40.97  E-value: 3.43e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1005571659 135 PASGKVMQGF---QKNGQGVFVQTATNAKVESVNEGLVVFAGKK-EELGNTVQIQHADGTESWYANLNDMSVKLYDYVSK 210
Cdd:PRK10871  203 PTDGKVIENFsasEGGNKGIDIAGSKGQAIIATADGRVVYAGNAlRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKA 282

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1005571659 211 KQKIGTVSNdTNDKNGKFYFAIKKNEKFIDPIQ 243
Cdd:PRK10871  283 GQKIATMGS-TGTSSTRLHFEIRYKGKSVNPLR 314
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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