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Conserved domains on  [gi|503790961|ref|WP_014024955|]
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PTS glucose transporter subunit IIA [Lactococcus garvieae]

Protein Classification

PTS glucose transporter subunit IIA( domain architecture ID 10005511)

PTS glucose transporter subunit IIA is part of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system that catalyzes the phosphorylation of incoming glucose substrates concomitantly with their translocation across the cell membrane

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NagE COG2190
Phosphotransferase system IIA component [Carbohydrate transport and metabolism];
9-160 1.50e-60

Phosphotransferase system IIA component [Carbohydrate transport and metabolism];


:

Pssm-ID: 441793 [Multi-domain]  Cd Length: 154  Bit Score: 184.17  E-value: 1.50e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961   9 SVSEDKNLYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLVQ--GHAIGFKRADGLEVLLHLGID 86
Cdd:COG2190    1 KKSKTLTIYAPVSGEVVPLSEVPDPVFASKMMGDGVAIEPTGGKVVAPVDGTVTSVFptKHAIGLTSDDGLEVLIHIGID 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 503790961  87 TVSLNGQPFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITNTNEKLDSLTVKKGNAQAGDMLGQATA 160
Cdd:COG2190   81 TVKLNGEGFTVLVKEGDKVKAGDPLIEFDLDAIKAAGYSLITPVVVTNSDEYKSVELTKSGQVKAGDPLLTVKL 154
 
Name Accession Description Interval E-value
NagE COG2190
Phosphotransferase system IIA component [Carbohydrate transport and metabolism];
9-160 1.50e-60

Phosphotransferase system IIA component [Carbohydrate transport and metabolism];


Pssm-ID: 441793 [Multi-domain]  Cd Length: 154  Bit Score: 184.17  E-value: 1.50e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961   9 SVSEDKNLYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLVQ--GHAIGFKRADGLEVLLHLGID 86
Cdd:COG2190    1 KKSKTLTIYAPVSGEVVPLSEVPDPVFASKMMGDGVAIEPTGGKVVAPVDGTVTSVFptKHAIGLTSDDGLEVLIHIGID 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 503790961  87 TVSLNGQPFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITNTNEKLDSLTVKKGNAQAGDMLGQATA 160
Cdd:COG2190   81 TVKLNGEGFTVLVKEGDKVKAGDPLIEFDLDAIKAAGYSLITPVVVTNSDEYKSVELTKSGQVKAGDPLLTVKL 154
PTS_EIIA_1 pfam00358
phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1;
16-132 1.80e-52

phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1;


Pssm-ID: 459779 [Multi-domain]  Cd Length: 121  Bit Score: 162.55  E-value: 1.80e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961   16 LYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLVQ--GHAIGFKRADGLEVLLHLGIDTVSLNGQ 93
Cdd:pfam00358   3 IYSPVSGEVIPLSEVPDPVFSQKMMGDGVAIEPSEGKVYAPVDGTVTTVFptKHAIGITTDDGVEVLIHIGIDTVKLKGE 82

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 503790961   94 PFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILI 132
Cdd:pfam00358  83 GFESLVKEGDKVKAGDLLLEFDLEAIKAAGYSTTTPVVV 121
PTS_IIA_glc cd00210
PTS_IIA, PTS system, glucose/sucrose specific IIA subunit. The bacterial phosphoenolpyruvate: ...
 16-137 6.82e-43

PTS_IIA, PTS system, glucose/sucrose specific IIA subunit. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. This family is one of four structurally and functionally distinct group IIA PTS system cytoplasmic enzymes, necessary for the uptake of carbohydrates across the cytoplasmic membrane and their phosphorylation.


Pssm-ID: 238128 [Multi-domain]  Cd Length: 124  Bit Score: 138.58  E-value: 6.82e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961  16 LYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLV--QGHAIGFKRADGLEVLLHLGIDTVSLNGQ 93
Cdd:cd00210    1 LASPITGEIVPLDQVPDEVFASKMMGDGFAIKPSDGKVVAPVDGTIVQIfpTKHAIGIESDSGVEILIHIGIDTVKLNGE 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 503790961  94 PFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITNTNE 137
Cdd:cd00210   81 GFTSHVEEGQRVKQGDKLLEFDLPAIKAAGLDDTTPVIVTNSDE 124
PTBA TIGR00830
PTS system, glucose subfamily, IIA component; These are part of the The PTS Glucose-Glucoside ...
 16-134 9.19e-42

PTS system, glucose subfamily, IIA component; These are part of the The PTS Glucose-Glucoside (Glc) SuperFamily. The Glc family includes permeases specific for glucose, N-acetylglucosamine and a large variety of a- and b-glucosides. However, not all b-glucoside PTS permeases are in this class, as the cellobiose (Cel) b-glucoside PTS permease is in the Lac family (TC #4.A.3). The IIA, IIB and IIC domains of all of the permeases listed below are demonstrably homologous. These permeases show limited sequence similarity with members of the Fru family (TC #4.A.2). Several of the PTS permeases in the Glc family lack their own IIA domains and instead use the glucose IIA protein (IIAglc or Crr). Most of these permeases have the B and C domains linked together in a single polypeptide chain, and a cysteyl residue in the IIB domain is phosphorylated by direct phosphoryl transfer from IIAglc(his~P). Those permeases which lack a IIA domain include the maltose (Mal), arbutin-salicin-cellobiose (ASC), trehalose (Tre), putative glucoside (Glv) and sucrose (Scr) permeases of E. coli . Most, but not all Scr permeases of other bacteria also lack a IIA domain. The three-dimensional structures of the IIA and IIB domains of the E. coli glucose permease have been elucidated. IIAglchas a complex b-sandwich structure while IIBglc is a split ab-sandwich with a topology unrelated to the split ab-sandwich structure of HPr. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 273289 [Multi-domain]  Cd Length: 121  Bit Score: 135.45  E-value: 9.19e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961   16 LYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLVQ--GHAIGFKRADGLEVLLHLGIDTVSLNGQ 93
Cdd:TIGR00830   1 IVSPISGEIVPLDQVPDEVFASKIVGDGFAILPTDGKVVAPVDGKIGKIFptKHAFGIESDNGVEILIHIGIDTVKLNGE 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 503790961   94 PFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITN 134
Cdd:TIGR00830  81 GFTSHVEEGDRVKKGDPLLEFDLKAIKKKGYSITTPVVVTN 121
PRK09824 PRK09824
PTS system beta-glucoside-specific transporter subunits IIABC; Provisional
 11-155 1.23e-39

PTS system beta-glucoside-specific transporter subunits IIABC; Provisional


Pssm-ID: 236627 [Multi-domain]  Cd Length: 627  Bit Score: 141.00  E-value: 1.23e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961  11 SEDKNLYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEV-SLVQ-GHAIGFKRADGLEVLLHLGIDTV 88
Cdd:PRK09824 476 PEQGGICSPMTGEVVPLEQVADTTFASGLLGKGIAILPSVGEVRSPVAGRVaSLFAtLHAIGLESDDGVEVLIHVGIDTV 555

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 503790961  89 SLNGQPFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITNTNEKLDSLTVKKGNAQAGDML 155
Cdd:PRK09824 556 KLDGKFFTAHVNVGDKVNTGDLLIEFDIPAIREAGYDLTTPVLISNSDDYTDVLPHATAQVSAGEPL 622
 
Name Accession Description Interval E-value
NagE COG2190
Phosphotransferase system IIA component [Carbohydrate transport and metabolism];
9-160 1.50e-60

Phosphotransferase system IIA component [Carbohydrate transport and metabolism];


Pssm-ID: 441793 [Multi-domain]  Cd Length: 154  Bit Score: 184.17  E-value: 1.50e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961   9 SVSEDKNLYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLVQ--GHAIGFKRADGLEVLLHLGID 86
Cdd:COG2190    1 KKSKTLTIYAPVSGEVVPLSEVPDPVFASKMMGDGVAIEPTGGKVVAPVDGTVTSVFptKHAIGLTSDDGLEVLIHIGID 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 503790961  87 TVSLNGQPFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITNTNEKLDSLTVKKGNAQAGDMLGQATA 160
Cdd:COG2190   81 TVKLNGEGFTVLVKEGDKVKAGDPLIEFDLDAIKAAGYSLITPVVVTNSDEYKSVELTKSGQVKAGDPLLTVKL 154
PTS_EIIA_1 pfam00358
phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1;
16-132 1.80e-52

phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1;


Pssm-ID: 459779 [Multi-domain]  Cd Length: 121  Bit Score: 162.55  E-value: 1.80e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961   16 LYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLVQ--GHAIGFKRADGLEVLLHLGIDTVSLNGQ 93
Cdd:pfam00358   3 IYSPVSGEVIPLSEVPDPVFSQKMMGDGVAIEPSEGKVYAPVDGTVTTVFptKHAIGITTDDGVEVLIHIGIDTVKLKGE 82

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 503790961   94 PFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILI 132
Cdd:pfam00358  83 GFESLVKEGDKVKAGDLLLEFDLEAIKAAGYSTTTPVVV 121
PTS_IIA_glc cd00210
PTS_IIA, PTS system, glucose/sucrose specific IIA subunit. The bacterial phosphoenolpyruvate: ...
 16-137 6.82e-43

PTS_IIA, PTS system, glucose/sucrose specific IIA subunit. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. This family is one of four structurally and functionally distinct group IIA PTS system cytoplasmic enzymes, necessary for the uptake of carbohydrates across the cytoplasmic membrane and their phosphorylation.


Pssm-ID: 238128 [Multi-domain]  Cd Length: 124  Bit Score: 138.58  E-value: 6.82e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961  16 LYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLV--QGHAIGFKRADGLEVLLHLGIDTVSLNGQ 93
Cdd:cd00210    1 LASPITGEIVPLDQVPDEVFASKMMGDGFAIKPSDGKVVAPVDGTIVQIfpTKHAIGIESDSGVEILIHIGIDTVKLNGE 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 503790961  94 PFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITNTNE 137
Cdd:cd00210   81 GFTSHVEEGQRVKQGDKLLEFDLPAIKAAGLDDTTPVIVTNSDE 124
PTBA TIGR00830
PTS system, glucose subfamily, IIA component; These are part of the The PTS Glucose-Glucoside ...
 16-134 9.19e-42

PTS system, glucose subfamily, IIA component; These are part of the The PTS Glucose-Glucoside (Glc) SuperFamily. The Glc family includes permeases specific for glucose, N-acetylglucosamine and a large variety of a- and b-glucosides. However, not all b-glucoside PTS permeases are in this class, as the cellobiose (Cel) b-glucoside PTS permease is in the Lac family (TC #4.A.3). The IIA, IIB and IIC domains of all of the permeases listed below are demonstrably homologous. These permeases show limited sequence similarity with members of the Fru family (TC #4.A.2). Several of the PTS permeases in the Glc family lack their own IIA domains and instead use the glucose IIA protein (IIAglc or Crr). Most of these permeases have the B and C domains linked together in a single polypeptide chain, and a cysteyl residue in the IIB domain is phosphorylated by direct phosphoryl transfer from IIAglc(his~P). Those permeases which lack a IIA domain include the maltose (Mal), arbutin-salicin-cellobiose (ASC), trehalose (Tre), putative glucoside (Glv) and sucrose (Scr) permeases of E. coli . Most, but not all Scr permeases of other bacteria also lack a IIA domain. The three-dimensional structures of the IIA and IIB domains of the E. coli glucose permease have been elucidated. IIAglchas a complex b-sandwich structure while IIBglc is a split ab-sandwich with a topology unrelated to the split ab-sandwich structure of HPr. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 273289 [Multi-domain]  Cd Length: 121  Bit Score: 135.45  E-value: 9.19e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961   16 LYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLVQ--GHAIGFKRADGLEVLLHLGIDTVSLNGQ 93
Cdd:TIGR00830   1 IVSPISGEIVPLDQVPDEVFASKIVGDGFAILPTDGKVVAPVDGKIGKIFptKHAFGIESDNGVEILIHIGIDTVKLNGE 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 503790961   94 PFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITN 134
Cdd:TIGR00830  81 GFTSHVEEGDRVKKGDPLLEFDLKAIKKKGYSITTPVVVTN 121
PTS-II-ABC-beta TIGR01995
PTS system, beta-glucoside-specific IIABC component; This model represents a family of PTS ...
 7-155 1.46e-41

PTS system, beta-glucoside-specific IIABC component; This model represents a family of PTS enzyme II proteins in which all three domains are found in the same polypeptide chain and which appear to have a broad specificity for beta-glucosides including salicin (beta-D-glucose-1-salicylate) and arbutin (Hydroquinone-O-beta-D-glucopyranoside). These are distinct from the closely related sucrose-specific and trehalose-specific PTS transporters.


Pssm-ID: 273919 [Multi-domain]  Cd Length: 610  Bit Score: 145.96  E-value: 1.46e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961    7 KKSVSEDKNLYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLVQ--GHAIGFKRADGLEVLLHLG 84
Cdd:TIGR01995 456 AAALSKDESLYAPVAGEMLPLNEVPDEVFSSGAMGKGIAILPTEGEVVAPVDGTVTAVFptKHAIGIRSDNGIEILIHVG 535

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 503790961   85 IDTVSLNGQPFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITNTNEKLDSLTVKKGNAQAGDML 155
Cdd:TIGR01995 536 IDTVELNGEGFEILVKVGDHVKAGQLLLTFDLDKIKEAGYDPTTPVVVTNTKDFLDVIPTDKETVTAGDVL 606
PRK09824 PRK09824
PTS system beta-glucoside-specific transporter subunits IIABC; Provisional
 11-155 1.23e-39

PTS system beta-glucoside-specific transporter subunits IIABC; Provisional


Pssm-ID: 236627 [Multi-domain]  Cd Length: 627  Bit Score: 141.00  E-value: 1.23e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961  11 SEDKNLYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEV-SLVQ-GHAIGFKRADGLEVLLHLGIDTV 88
Cdd:PRK09824 476 PEQGGICSPMTGEVVPLEQVADTTFASGLLGKGIAILPSVGEVRSPVAGRVaSLFAtLHAIGLESDDGVEVLIHVGIDTV 555

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 503790961  89 SLNGQPFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITNTNEKLDSLTVKKGNAQAGDML 155
Cdd:PRK09824 556 KLDGKFFTAHVNVGDKVNTGDLLIEFDIPAIREAGYDLTTPVLISNSDDYTDVLPHATAQVSAGEPL 622
PRK09439 PRK09439
PTS system glucose-specific transporter subunit; Provisional
 6-153 1.57e-24

PTS system glucose-specific transporter subunit; Provisional


Pssm-ID: 181859 [Multi-domain]  Cd Length: 169  Bit Score: 93.12  E-value: 1.57e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961   6 KKKSVSEDKNLYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLV--QGHAIGFKRADGLEVLLHL 83
Cdd:PRK09439  13 DDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPTGNKMVAPVDGTIGKIfeTNHAFSIESDSGVELFVHF 92

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961  84 GIDTVSLNGQPFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITNTnEKLDSLTVKKGNAQAGD 153
Cdd:PRK09439  93 GIDTVELKGEGFKRIAEEGQRVKVGDPIIEFDLPLLEEKAKSTLTPVVISNM-DEIKELIKLSGSVTAGE 161
PRK10255 PRK10255
PTS system N-acetyl glucosamine specific transporter subunits IIABC; Provisional
 16-153 2.38e-20

PTS system N-acetyl glucosamine specific transporter subunits IIABC; Provisional


Pssm-ID: 182338 [Multi-domain]  Cd Length: 648  Bit Score: 86.46  E-value: 2.38e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961  16 LYAPLAGTVIDLEKVSDPVFAKKVMGDGYAIEPTSSEIVSPVAGEVSLV--QGHAIGFKRADGLEVLLHLGIDTVSLNGQ 93
Cdd:PRK10255 501 LVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIfnTNHAFCLETEKGAEIVVHMGIDTVALEGK 580

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 503790961  94 PFQIKVKVGDRVEGGDALGQVNWKEVEAAGLALTSMILITNTNEKLDSLTVKKGNAQAGD 153
Cdd:PRK10255 581 GFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQ 640
ATP-synt_ab_Xtn pfam16886
ATPsynthase alpha/beta subunit N-term extension; ATP-synt_ab_Xtn is an extension of the ...
 84-114 7.78e-05

ATPsynthase alpha/beta subunit N-term extension; ATP-synt_ab_Xtn is an extension of the alpha-beta catalytic subunit of VATA or V-type proton ATPase catalytic subunit at the N-terminal end. It is found from bacteria to humans, and was not modelled in family ATP-synt_ab, pfam00006.


Pssm-ID: 465299 [Multi-domain]  Cd Length: 120  Bit Score: 40.07  E-value: 7.78e-05
                          10        20        30
                  ....*....|....*....|....*....|....
gi 503790961   84 GIDTVSLNGQ---PFQIKVKVGDRVEGGDALGQV 114
Cdd:pfam16886  22 GVDVPALDREkkwEFTPTVKVGDKVSGGDILGTV 55
PRK04192 PRK04192
V-type ATP synthase subunit A; Provisional
 84-114 1.73e-03

V-type ATP synthase subunit A; Provisional


Pssm-ID: 235248 [Multi-domain]  Cd Length: 586  Bit Score: 37.84  E-value: 1.73e-03
                         10        20        30
                 ....*....|....*....|....*....|....
gi 503790961  84 GIDTVSLNGQ---PFQIKVKVGDRVEGGDALGQV 114
Cdd:PRK04192 106 GVYVPALDREkkwEFTPTVKVGDKVEAGDILGTV 139
M23_peptidase cd12797
M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins; ...
 48-114 8.10e-03

M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins; This model describes the metallopeptidase M23 family, which includes beta-lytic metallopeptidase and lysostaphin. Members of this family are zinc endopeptidases that lyse bacterial cell wall peptidoglycans; they cleave either the N-acylmuramoyl-Ala bond between the cell wall peptidoglycan and the cross-linking peptide (e.g. beta-lytic endopeptidase) or a bond within the cross-linking peptide (e.g. stapholysin, and lysostaphin). Beta-lytic metallopeptidase, formerly known as beta-lytic protease, has a preference for cleavage of Gly-X bonds and favors hydrophobic or apolar residues on either side. It inhibits growth of sensitive organisms and may potentially serve as an antimicrobial agent. Lysostaphin, produced by Staphylococcus genus, cleaves pentaglycine cross-bridges of cell wall peptidoglycan, acting as autolysins to maintain cell wall metabolism or as toxins and weapons against competing strains. Staphylolysin (also known as LasA) is implicated in a range of processes related to Pseudomonas virulence, including stimulating shedding of the ectodomain of cell surface heparan sulphate proteoglycan syndecan-1, and elastin degradation in connective tissue. Its active site is less constricted and contains a five-coordinate zinc ion with trigonal bipyramidal geometry and two metal-bound water molecules, possibly contributing to its activity against a wider range of substrates than those used by related lytic enzymes, consistent with its multiple roles in Pseudomonas virulence. The family includes members that do not appear to have the conserved zinc-binding site and might be lipoproteins lacking proteolytic activity.


Pssm-ID: 410984 [Multi-domain]  Cd Length: 85  Bit Score: 33.72  E-value: 8.10e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 503790961  48 PTSSEIVSPVAGEVSlvqghAIGFKRADGLEVLLHLGIDTVSLNGQPFQIKVKVGDRVEGGDALGQV 114
Cdd:cd12797    9 PEGTPVYAAADGTVV-----FAGWDGGYGNYVIIDHGNGYYTLYAHLSSILVKVGQRVKKGQVIGTV 70
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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