NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|493445302|ref|WP_006400672|]
View 

MULTISPECIES: transcription termination/antitermination protein NusG [Burkholderia]

Protein Classification

transcription termination/antitermination protein NusG( domain architecture ID 11489978)

transcription termination/antitermination protein NusG (N-Utilization Substance G) is involved in transcription elongation and termination in bacteria

CATH:  2.30.30.30|3.30.70.940
Gene Ontology:  GO:0006353|GO:0032784|GO:0031564|GO:0006354
PubMed:  27899640|29887376
SCOP:  4002609

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
nusG TIGR00922
transcription termination/antitermination factor NusG; NusG proteins are transcription factors ...
 13-184 7.80e-85

transcription termination/antitermination factor NusG; NusG proteins are transcription factors which are aparrently universal in prokaryotes (archaea and eukaryotes have homologs that may have related functions). The essential components of these factors include an N-terminal RNP-like (ribonucleoprotein) domain and a C-terminal KOW motif (pfam00467) believed to be a nucleic acid binding domain. In E. coli, NusA has been shown to interact with RNA polymerase and termination factor Rho. This model covers a wide variety of bacterial species but excludes mycoplasmas which are covered by a separate model (TIGR01956).The function of all of these NusG proteins is likely to be the same at the level of interaction with RNA and other protein factors to affect termination; however different species may utilize NusG towards different processes and in combination with different suites of affector proteins.In E. coli, NusG promotes rho-dependent termination. It is an essential gene. In Streptomyces virginiae and related species, an additional N-terminal sequence is also present and is suggested to play a role in butyrolactone-mediated autoregulation. In Thermotoga maritima, NusG has a long insert, fails to substitute for E. coli NusG (with or without the long insert), is a large 0.7 % of total cellular protein, and has a general, sequence non-specific DNA and RNA binding activity that blocks ethidium staining, yet permits transcription.Archaeal proteins once termed NusG share the KOW domain but are actually a ribosomal protein corresponding to L24p in bacterial and L26e in eukaryotes (TIGR00405). [Transcription, Transcription factors]


:

Pssm-ID: 273341 [Multi-domain]  Cd Length: 172  Bit Score: 247.60  E-value: 7.80e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   13 WYVVHAYSGMEKSVQRALQERIERAGMQDKFGQILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTAK 92
Cdd:TIGR00922   1 WYVVQTYSGYEKKVKQNLEELIELLGMGDYIFEVIVPTEEVVEIKKGKKKVVERKIFPGYVLVKMDLTDVSWHLVKNTPG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   93 VTGFVGGARNRPTPISPKEVEKIMSQMQEGVEKPRPKTLFEVGEMVRVKEGPFTDFNGTVEEVNYEKSRVRVSVTIFGRS 172
Cdd:TIGR00922  81 VTGFVGSGGKPKALSEDEEVKNILNALEEGKDKPKPKIDFEVGEQVRVNDGPFANFTGTVEEVDYEKSKLKVSVSIFGRE 160

                         170
                  ....*....|..
gi 493445302  173 TPVELEFGQVEK 184
Cdd:TIGR00922 161 TPVELEFSQVEK 172
 
Name Accession Description Interval E-value
nusG TIGR00922
transcription termination/antitermination factor NusG; NusG proteins are transcription factors ...
 13-184 7.80e-85

transcription termination/antitermination factor NusG; NusG proteins are transcription factors which are aparrently universal in prokaryotes (archaea and eukaryotes have homologs that may have related functions). The essential components of these factors include an N-terminal RNP-like (ribonucleoprotein) domain and a C-terminal KOW motif (pfam00467) believed to be a nucleic acid binding domain. In E. coli, NusA has been shown to interact with RNA polymerase and termination factor Rho. This model covers a wide variety of bacterial species but excludes mycoplasmas which are covered by a separate model (TIGR01956).The function of all of these NusG proteins is likely to be the same at the level of interaction with RNA and other protein factors to affect termination; however different species may utilize NusG towards different processes and in combination with different suites of affector proteins.In E. coli, NusG promotes rho-dependent termination. It is an essential gene. In Streptomyces virginiae and related species, an additional N-terminal sequence is also present and is suggested to play a role in butyrolactone-mediated autoregulation. In Thermotoga maritima, NusG has a long insert, fails to substitute for E. coli NusG (with or without the long insert), is a large 0.7 % of total cellular protein, and has a general, sequence non-specific DNA and RNA binding activity that blocks ethidium staining, yet permits transcription.Archaeal proteins once termed NusG share the KOW domain but are actually a ribosomal protein corresponding to L24p in bacterial and L26e in eukaryotes (TIGR00405). [Transcription, Transcription factors]


Pssm-ID: 273341 [Multi-domain]  Cd Length: 172  Bit Score: 247.60  E-value: 7.80e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   13 WYVVHAYSGMEKSVQRALQERIERAGMQDKFGQILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTAK 92
Cdd:TIGR00922   1 WYVVQTYSGYEKKVKQNLEELIELLGMGDYIFEVIVPTEEVVEIKKGKKKVVERKIFPGYVLVKMDLTDVSWHLVKNTPG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   93 VTGFVGGARNRPTPISPKEVEKIMSQMQEGVEKPRPKTLFEVGEMVRVKEGPFTDFNGTVEEVNYEKSRVRVSVTIFGRS 172
Cdd:TIGR00922  81 VTGFVGSGGKPKALSEDEEVKNILNALEEGKDKPKPKIDFEVGEQVRVNDGPFANFTGTVEEVDYEKSKLKVSVSIFGRE 160

                         170
                  ....*....|..
gi 493445302  173 TPVELEFGQVEK 184
Cdd:TIGR00922 161 TPVELEFSQVEK 172
NusG COG0250
Transcription termination/antitermination protein NusG [Transcription];
11-185 2.46e-80

Transcription termination/antitermination protein NusG [Transcription];


Pssm-ID: 440020 [Multi-domain]  Cd Length: 171  Bit Score: 235.87  E-value: 2.46e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  11 KRWYVVHAYSGMEKSVQRALQERIEraGMQdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNT 90
Cdd:COG0250    5 KRWYVVHTYSGYEKKVKENLERRIE--GIE-----VFVPTEEVVEIKNGKKKTVERPLFPGYVFVRMDLTDESWYLVRNT 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  91 AKVTGFVGGArNRPTPISPKEVEKIMSQMQEGVEKPRPKTLFEVGEMVRVKEGPFTDFNGTVEEVNYEKSRVRVSVTIFG 170
Cdd:COG0250   78 PGVTGFVGFG-GKPAPLPDEEVERILARLEEGEEKPRPKVDFEVGDRVRITDGPFAGFEGTVEEVDPEKGRVKVLVSIFG 156

                        170
                 ....*....|....*
gi 493445302 171 RSTPVELEFGQVEKV 185
Cdd:COG0250  157 RETPVELDFSQVEKI 171
NGN_Bact_1 cd09891
Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The ...
 12-119 8.69e-57

Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination in bacteria. NusG is essential in Escherichia coli and associates with RNA polymerase elongation and Rho-termination. Homologs of the NusG gene exist in all bacteria. The NusG N-terminal domain (NGN) is similar in all NusG homologs, but its C-terminal domain and the linker that separates these two domains are different. The domain organization of NusG suggests that the common properties of NusG and its homologs are due to their similar NGN domains.


Pssm-ID: 193580 [Multi-domain]  Cd Length: 107  Bit Score: 174.20  E-value: 8.69e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  12 RWYVVHAYSGMEKSVQRALQERIERAGMQDKFGQILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTA 91
Cdd:cd09891    1 KWYVVHTYSGYENKVKENLEKRIESEGLEDYIGEVLVPTEEVVEVKNGKKKVKERKLFPGYVLVEMDMNDDTWHLVRNTP 80

                         90       100
                 ....*....|....*....|....*...
gi 493445302  92 KVTGFVGGArNRPTPISPKEVEKIMSQM 119
Cdd:cd09891   81 GVTGFVGSG-GKPVPLSEEEVERILGQV 107
NGN smart00738
In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, ...
 12-118 5.70e-38

In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, this domain may confer affinity for Spt4p.Spt4p


Pssm-ID: 197850 [Multi-domain]  Cd Length: 106  Bit Score: 126.33  E-value: 5.70e-38
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302    12 RWYVVHAYSGMEKSVQRALQERIERAGMQDKFGQILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTA 91
Cdd:smart00738   1 NWYAVRTTSGQEKRVAENLERKAEALGLEDKIVSILVPTEEVKEIRRGKKKVVERKLFPGYIFVEADLEDEVWTAIRGTP 80

                           90       100
                   ....*....|....*....|....*..
gi 493445302    92 KVTGFVGGArNRPTPISPKEVEKIMSQ 118
Cdd:smart00738  81 GVRGFVGGG-GKPTPVPDDEIEKILKP 106
NusG pfam02357
Transcription termination factor nusG;
11-116 1.90e-36

Transcription termination factor nusG;


Pssm-ID: 426736 [Multi-domain]  Cd Length: 96  Bit Score: 121.95  E-value: 1.90e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   11 KRWYVVHAYSGMEKSVQRALqeriERAGMQdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNT 90
Cdd:pfam02357   1 KKWYVLQTYSGKEKKVKENL----ERQGIE-----VFLPTEEVVEIRNGKKKVVERPLFPGYVFVRMDMTDETWHLVRST 71

                          90       100
                  ....*....|....*....|....*.
gi 493445302   91 AKVTGFVGGArNRPTPISPKEVEKIM 116
Cdd:pfam02357  72 PGVTGFVGGS-GKPTPIPDEEVERIL 96
antiterm_LoaP NF033641
antiterminator LoaP; LoaP is a paralog of NusG with an extensive presence in Firmicutes. The ...
 13-184 1.85e-25

antiterminator LoaP; LoaP is a paralog of NusG with an extensive presence in Firmicutes. The founding member, from Bacillus amyloliquefaciens, was shown to serve as an antiterminator for the transcription of genes involved in antibiotic biosynthesis.


Pssm-ID: 468124 [Multi-domain]  Cd Length: 166  Bit Score: 95.91  E-value: 1.85e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  13 WYVVHAYSGMEKSVQRALQERIERAGMQdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTAK 92
Cdd:NF033641   1 WYVLFVRTGKEEKVKKLLNKLFDKELLR-----CFVPKRKLPERKQGKWYKVIKPLFPGYVFIETDMNEELYYKLKKIPG 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  93 VTGFVGGARNRPTPISPKEVEKIMSQMQEG--VEkprPKTLFEVGEMVRVKEGPFTDFNGTVEEVNYEKSRVRVSVTIFG 170
Cdd:NF033641  76 IIRLLGNEGDEFSPIPEEEMEFLLKLLNDDdiIE---YSKGYIEGDKVIVISGPLKGLEGIIKKIDKRKRRAKIELNFMG 152

                        170
                 ....*....|....
gi 493445302 171 RSTPVELEFGQVEK 184
Cdd:NF033641 153 EERLVDVGIEIVSK 166
antiterm_UpxY NF033644
UpxY family transcription antiterminator; The UpxY family of NusG-related transcription ...
 13-166 4.53e-10

UpxY family transcription antiterminator; The UpxY family of NusG-related transcription antiterminators was described originally from a paralogous family of eight members from Bacteriodes fragilis, UpaY to UphY, each of which was associated with a distinct capsular polysaccharide biosynthesis locus. There is no UpxY protein per se.


Pssm-ID: 468125 [Multi-domain]  Cd Length: 162  Bit Score: 55.55  E-value: 4.53e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  13 WYVVHAYSGMEKSVQRALQER-IEragmqdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDEtWHLVKNTA 91
Cdd:NF033644   1 WYALYTRPRREKKVAELLEKKgIE----------SFLPMQKEIRQWSDRKKRVEVPLIPNLVFVHITEKEL-DEVLEQTP 69

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  92 KVTGFVGGARNRPTP--ISPKEVEKIM---SQMQEGVEKPRPKtlFEVGEMVRVKEGPFTDFNGTVEEVNyEKSRVRVSV 166
Cdd:NF033644  70 GVVRYIRDDRGKSKPaiIPDKQMERFRlmlDPSDEVVVYLEAP--LKKGDKVRVIGGPLKGLEGELVRVK-GKKRVVVRI 146
nusG PRK08559
transcription antitermination protein NusG; Validated
 6-166 1.48e-04

transcription antitermination protein NusG; Validated


Pssm-ID: 181467 [Multi-domain]  Cd Length: 153  Bit Score: 40.24  E-value: 1.48e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   6 ASPSGKRWYVVHAYSGMEKSVQRALQERIERAGMQDKfgQILVPTEevveVKGghkavterrffpgYVLVEMEMTDETWH 85
Cdd:PRK08559   1 MKPEMSMIFAVKTTAGQERNVALMLAMRAKKENLPIY--AILAPPE----LKG-------------YVLVEAESKGAVEE 61

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  86 LVKNTAKVTGFVGGarnrptPISPKEVEKIMSQMQEGVEkprpktlFEVGEMVRVKEGPFTDFNGTVEEVNYEKSRVRVS 165
Cdd:PRK08559  62 AIRGIPHVRGVVPG------EISFEEVEHFLKPKPIVEG-------IKEGDIVELIAGPFKGEKARVVRVDESKEEVTVE 128


                 .
gi 493445302 166 V 166
Cdd:PRK08559 129 L 129
 
Name Accession Description Interval E-value
nusG TIGR00922
transcription termination/antitermination factor NusG; NusG proteins are transcription factors ...
 13-184 7.80e-85

transcription termination/antitermination factor NusG; NusG proteins are transcription factors which are aparrently universal in prokaryotes (archaea and eukaryotes have homologs that may have related functions). The essential components of these factors include an N-terminal RNP-like (ribonucleoprotein) domain and a C-terminal KOW motif (pfam00467) believed to be a nucleic acid binding domain. In E. coli, NusA has been shown to interact with RNA polymerase and termination factor Rho. This model covers a wide variety of bacterial species but excludes mycoplasmas which are covered by a separate model (TIGR01956).The function of all of these NusG proteins is likely to be the same at the level of interaction with RNA and other protein factors to affect termination; however different species may utilize NusG towards different processes and in combination with different suites of affector proteins.In E. coli, NusG promotes rho-dependent termination. It is an essential gene. In Streptomyces virginiae and related species, an additional N-terminal sequence is also present and is suggested to play a role in butyrolactone-mediated autoregulation. In Thermotoga maritima, NusG has a long insert, fails to substitute for E. coli NusG (with or without the long insert), is a large 0.7 % of total cellular protein, and has a general, sequence non-specific DNA and RNA binding activity that blocks ethidium staining, yet permits transcription.Archaeal proteins once termed NusG share the KOW domain but are actually a ribosomal protein corresponding to L24p in bacterial and L26e in eukaryotes (TIGR00405). [Transcription, Transcription factors]


Pssm-ID: 273341 [Multi-domain]  Cd Length: 172  Bit Score: 247.60  E-value: 7.80e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   13 WYVVHAYSGMEKSVQRALQERIERAGMQDKFGQILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTAK 92
Cdd:TIGR00922   1 WYVVQTYSGYEKKVKQNLEELIELLGMGDYIFEVIVPTEEVVEIKKGKKKVVERKIFPGYVLVKMDLTDVSWHLVKNTPG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   93 VTGFVGGARNRPTPISPKEVEKIMSQMQEGVEKPRPKTLFEVGEMVRVKEGPFTDFNGTVEEVNYEKSRVRVSVTIFGRS 172
Cdd:TIGR00922  81 VTGFVGSGGKPKALSEDEEVKNILNALEEGKDKPKPKIDFEVGEQVRVNDGPFANFTGTVEEVDYEKSKLKVSVSIFGRE 160

                         170
                  ....*....|..
gi 493445302  173 TPVELEFGQVEK 184
Cdd:TIGR00922 161 TPVELEFSQVEK 172
NusG COG0250
Transcription termination/antitermination protein NusG [Transcription];
11-185 2.46e-80

Transcription termination/antitermination protein NusG [Transcription];


Pssm-ID: 440020 [Multi-domain]  Cd Length: 171  Bit Score: 235.87  E-value: 2.46e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  11 KRWYVVHAYSGMEKSVQRALQERIEraGMQdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNT 90
Cdd:COG0250    5 KRWYVVHTYSGYEKKVKENLERRIE--GIE-----VFVPTEEVVEIKNGKKKTVERPLFPGYVFVRMDLTDESWYLVRNT 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  91 AKVTGFVGGArNRPTPISPKEVEKIMSQMQEGVEKPRPKTLFEVGEMVRVKEGPFTDFNGTVEEVNYEKSRVRVSVTIFG 170
Cdd:COG0250   78 PGVTGFVGFG-GKPAPLPDEEVERILARLEEGEEKPRPKVDFEVGDRVRITDGPFAGFEGTVEEVDPEKGRVKVLVSIFG 156

                        170
                 ....*....|....*
gi 493445302 171 RSTPVELEFGQVEKV 185
Cdd:COG0250  157 RETPVELDFSQVEKI 171
NGN_Bact_1 cd09891
Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The ...
 12-119 8.69e-57

Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination in bacteria. NusG is essential in Escherichia coli and associates with RNA polymerase elongation and Rho-termination. Homologs of the NusG gene exist in all bacteria. The NusG N-terminal domain (NGN) is similar in all NusG homologs, but its C-terminal domain and the linker that separates these two domains are different. The domain organization of NusG suggests that the common properties of NusG and its homologs are due to their similar NGN domains.


Pssm-ID: 193580 [Multi-domain]  Cd Length: 107  Bit Score: 174.20  E-value: 8.69e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  12 RWYVVHAYSGMEKSVQRALQERIERAGMQDKFGQILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTA 91
Cdd:cd09891    1 KWYVVHTYSGYENKVKENLEKRIESEGLEDYIGEVLVPTEEVVEVKNGKKKVKERKLFPGYVLVEMDMNDDTWHLVRNTP 80

                         90       100
                 ....*....|....*....|....*...
gi 493445302  92 KVTGFVGGArNRPTPISPKEVEKIMSQM 119
Cdd:cd09891   81 GVTGFVGSG-GKPVPLSEEEVERILGQV 107
NGN smart00738
In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, ...
 12-118 5.70e-38

In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, this domain may confer affinity for Spt4p.Spt4p


Pssm-ID: 197850 [Multi-domain]  Cd Length: 106  Bit Score: 126.33  E-value: 5.70e-38
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302    12 RWYVVHAYSGMEKSVQRALQERIERAGMQDKFGQILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTA 91
Cdd:smart00738   1 NWYAVRTTSGQEKRVAENLERKAEALGLEDKIVSILVPTEEVKEIRRGKKKVVERKLFPGYIFVEADLEDEVWTAIRGTP 80

                           90       100
                   ....*....|....*....|....*..
gi 493445302    92 KVTGFVGGArNRPTPISPKEVEKIMSQ 118
Cdd:smart00738  81 GVRGFVGGG-GKPTPVPDDEIEKILKP 106
NusG pfam02357
Transcription termination factor nusG;
11-116 1.90e-36

Transcription termination factor nusG;


Pssm-ID: 426736 [Multi-domain]  Cd Length: 96  Bit Score: 121.95  E-value: 1.90e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   11 KRWYVVHAYSGMEKSVQRALqeriERAGMQdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNT 90
Cdd:pfam02357   1 KKWYVLQTYSGKEKKVKENL----ERQGIE-----VFLPTEEVVEIRNGKKKVVERPLFPGYVFVRMDMTDETWHLVRST 71

                          90       100
                  ....*....|....*....|....*.
gi 493445302   91 AKVTGFVGGArNRPTPISPKEVEKIM 116
Cdd:pfam02357  72 PGVTGFVGGS-GKPTPIPDEEVERIL 96
antiterm_LoaP NF033641
antiterminator LoaP; LoaP is a paralog of NusG with an extensive presence in Firmicutes. The ...
 13-184 1.85e-25

antiterminator LoaP; LoaP is a paralog of NusG with an extensive presence in Firmicutes. The founding member, from Bacillus amyloliquefaciens, was shown to serve as an antiterminator for the transcription of genes involved in antibiotic biosynthesis.


Pssm-ID: 468124 [Multi-domain]  Cd Length: 166  Bit Score: 95.91  E-value: 1.85e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  13 WYVVHAYSGMEKSVQRALQERIERAGMQdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTAK 92
Cdd:NF033641   1 WYVLFVRTGKEEKVKKLLNKLFDKELLR-----CFVPKRKLPERKQGKWYKVIKPLFPGYVFIETDMNEELYYKLKKIPG 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  93 VTGFVGGARNRPTPISPKEVEKIMSQMQEG--VEkprPKTLFEVGEMVRVKEGPFTDFNGTVEEVNYEKSRVRVSVTIFG 170
Cdd:NF033641  76 IIRLLGNEGDEFSPIPEEEMEFLLKLLNDDdiIE---YSKGYIEGDKVIVISGPLKGLEGIIKKIDKRKRRAKIELNFMG 152

                        170
                 ....*....|....
gi 493445302 171 RSTPVELEFGQVEK 184
Cdd:NF033641 153 EERLVDVGIEIVSK 166
KOW_NusG cd06091
NusG contains an NGN domain at its N-terminus and KOW motif at its C-terminus; KOW_NusG motif ...
 129-184 2.83e-24

NusG contains an NGN domain at its N-terminus and KOW motif at its C-terminus; KOW_NusG motif is one of the two domains of N-Utilization Substance G (NusG) a transcription elongation and Rho-termination factor in bacteria and archaea. KOW domain is known as an RNA-binding motif that is shared so far among some families of ribosomal proteins, the essential bacterial transcriptional elongation factor NusG, the eukaryotic chromatin elongation factor Spt5, the higher eukaryotic KIN17 proteins and Mtr4. The eukaryotic ortholog of NusG is Spt5 with multiple KOW motifs at its C-terminus.


Pssm-ID: 240515 [Multi-domain]  Cd Length: 56  Bit Score: 89.82  E-value: 2.83e-24
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 493445302 129 KTLFEVGEMVRVKEGPFTDFNGTVEEVNYEKSRVRVSVTIFGRSTPVELEFGQVEK 184
Cdd:cd06091    1 EVDFEVGDTVRIISGPFAGFEGKVEEIDEEKGKVKVLVEMFGRETPVELDFDQVEK 56
NGN cd08000
N-Utilization Substance G (NusG) N-terminal (NGN) domain Superfamily; The N-Utilization ...
 13-115 3.45e-19

N-Utilization Substance G (NusG) N-terminal (NGN) domain Superfamily; The N-Utilization Substance G (NusG) and its eukaryotic homolog Spt5 are involved in transcription elongation and termination. NusG contains an NGN domain at its N-terminus and Kyrpides Ouzounis and Woese (KOW) repeats at its C-terminus in bacteria and archaea. The eukaryotic ortholog, Spt5, is a large protein composed of an acidic N-terminus, an NGN domain, and multiple KOW motifs at its C-terminus. Spt5 forms a Spt4-Spt5 complex that is an essential RNA Polymerase II elongation factor. NusG was originally discovered as an N-dependent antitermination enhancing activity in Escherichia coli and has a variety of functions, such as being involved in RNA polymerase elongation and Rho-termination in bacteria. Orthologs of the NusG gene exist in all bacteria, but its functions and requirements are different. The diverse activities suggest that, after diverging from a common ancestor, NusG proteins became specialized in different bacteria.


Pssm-ID: 193574 [Multi-domain]  Cd Length: 99  Bit Score: 78.13  E-value: 3.45e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  13 WYVVHAYSGMEKSVQRALQERIERAGMQdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTAK 92
Cdd:cd08000    2 WYVLFVKTGREEKVEKLLEKRFEANDIE-----AFVPKKEVPERKRGKIEEVIKPLFPGYVFVETDLSPELYELIREVPG 76

                         90       100
                 ....*....|....*....|...
gi 493445302  93 VTGFVGGArNRPTPISPKEVEKI 115
Cdd:cd08000   77 VIGILGNG-EEPSPVSDEEIEMI 98
NusG_myco TIGR01956
NusG family protein; This model represents a family of Mycoplasma proteins orthologous to the ...
 12-184 8.83e-18

NusG family protein; This model represents a family of Mycoplasma proteins orthologous to the bacterial transcription termination/antitermination factor NusG. These sequences from Mycoplasma are notably diverged (long branches in a Neighbor-joining phylogenetic tree) from the bacterial species. And although NusA and ribosomal protein S10 (NusE) appear to be present, NusB may be absent in Mycoplasmas calling into question whether these species have a functional Nus system including this family as a member.


Pssm-ID: 273895 [Multi-domain]  Cd Length: 258  Bit Score: 78.08  E-value: 8.83e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   12 RWYVVHAYSGMEKSVQRALQERIERAGMQDKFGQILVPTEEVVEVK----GGHKA------------------------V 63
Cdd:TIGR01956   1 QWYIATTINGNEDEVIENIKAKVRALGLENYISDFKILKEREIEEKvfepKNGQAprsmkntattkwetldetkykktkI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   64 TERRFFPGYVLVEMEMTDETWHLVKNTAKVTGFVG--GARNRPTPISPKEVEKIMSQM-------------QEGVEKPRP 128
Cdd:TIGR01956  81 SEKNKYNGYIYIKMIMTEDAWFLIRNTENVTGLVGssGKGAKPIPISADADKLKMLKGisentkkrvlvtnTAIVEMEEN 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  129 K------------------------------------------TLFEVGEMVRVKEGPFTDFNGTVEEVNYEKSRVRVSV 166
Cdd:TIGR01956 161 KfdekcqyilkhkqvkpeaiaqvsesgeiideiveefqlvdnlSKFRVGNFVKIVDGPFKGIVGKIKKIDQEKKKAIVEV 240

                         250
                  ....*....|....*...
gi 493445302  167 TIFGRSTPVELEFGQVEK 184
Cdd:TIGR01956 241 EILGKSVDVDLNFKHLKL 258
antiterm_UpxY NF033644
UpxY family transcription antiterminator; The UpxY family of NusG-related transcription ...
 13-166 4.53e-10

UpxY family transcription antiterminator; The UpxY family of NusG-related transcription antiterminators was described originally from a paralogous family of eight members from Bacteriodes fragilis, UpaY to UphY, each of which was associated with a distinct capsular polysaccharide biosynthesis locus. There is no UpxY protein per se.


Pssm-ID: 468125 [Multi-domain]  Cd Length: 162  Bit Score: 55.55  E-value: 4.53e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  13 WYVVHAYSGMEKSVQRALQER-IEragmqdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDEtWHLVKNTA 91
Cdd:NF033644   1 WYALYTRPRREKKVAELLEKKgIE----------SFLPMQKEIRQWSDRKKRVEVPLIPNLVFVHITEKEL-DEVLEQTP 69

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  92 KVTGFVGGARNRPTP--ISPKEVEKIM---SQMQEGVEKPRPKtlFEVGEMVRVKEGPFTDFNGTVEEVNyEKSRVRVSV 166
Cdd:NF033644  70 GVVRYIRDDRGKSKPaiIPDKQMERFRlmlDPSDEVVVYLEAP--LKKGDKVRVIGGPLKGLEGELVRVK-GKKRVVVRI 146
NGN_plant cd09890
Plant N-Utilization Substance G (NusG) N-terminal (NGN) domain; The N-Utilization Substance G ...
 13-116 1.99e-09

Plant N-Utilization Substance G (NusG) N-terminal (NGN) domain; The N-Utilization Substance G (NusG) protein and its eukaryotic homolog, Spt5, are involved in transcription elongation and termination. NusG contains a NGN domain at its N-terminus and Kyrpides Ouzounis and Woese (KOW) repeats at its C-terminus in bacteria and archaea. The eukaryotic ortholog, Spt5, is a large protein comprising an acidic N-terminus, an NGN domain, and multiple KOW motifs at its C-terminus. Spt5 forms an Spt4-Spt5 complex that is an essential RNA polymerase II elongation factor. The bacterial infected plants contain bacterial DNA, such as NGN sequences, that can be used to clone the DNA of uncultured organisms.


Pssm-ID: 193579  Cd Length: 113  Bit Score: 52.74  E-value: 1.99e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  13 WYVVHAYSGMEKSVQRALQERIERAGMQDKFgQILVPTEEV-VEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTA 91
Cdd:cd09890    2 WYMLRVPAGRENQAAEALERALATEFPDREF-EVWVPSIPVdRKLKNGSISVKEKPLFPGYVLLRCVLNKEVYDFIRDND 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 493445302  92 KVTGFVGGA--------RNRPTPISPKEVEKIM 116
Cdd:cd09890   81 SVYGFVGSKvgktgkrqIEIPRPVPVEEIEAIF 113
NGN_Bact_2 cd09889
Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 2; The ...
 12-115 6.95e-07

Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 2; The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination. NusG is essential in Escherichia coli and associates with RNA polymerase elongation and Rho-termination. Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. The NusG N-terminal domain (NGN) is quite similar in all NusG orthologs, but its C-terminal domain and the linker that separates these two domains are different. The domain organization of NusG and its orthologs suggests that the common properties of NusG and its orthologs and paralogs are due to their similar NGN domains.


Pssm-ID: 193578 [Multi-domain]  Cd Length: 100  Bit Score: 45.78  E-value: 6.95e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  12 RWYVVHAYSGMEKSVQRALQERIERAGMQDKFgqilVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKnta 91
Cdd:cd09889    1 MWYVVQVRTGREKAVLELLEKLVGPDVLQECF----IPQYERKKRSQGVWRERKYTLFPGYVFVVTDDIDELYYELK--- 73

                         90       100
                 ....*....|....*....|....*.
gi 493445302  92 KVTGFVG--GARNRPTPISPKEVEKI 115
Cdd:cd09889   74 RVPGFTRllGNDGSFFPLTPEEADFI 99
NGN_SP cd09886
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP); The ...
 12-116 1.67e-06

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP); The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination. NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination in bacteria. Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. The NusG N-terminal (NGN) domain is quite similar in all NusG orthologs, but its C-terminal domains and the linker that separate these two domains are different. The domain organization of NusG and its orthologs suggest that the common properties of NusG and its orthologs and paralogs are due to their similar NGN domains.


Pssm-ID: 193575 [Multi-domain]  Cd Length: 97  Bit Score: 44.67  E-value: 1.67e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  12 RWYVVHAYSGMEKSVQRALQERIERAgmqdkfgqiLVPTEEVVEVKGGHKAVTERRFFPGYVLVEM-EMTDETWHLVKNT 90
Cdd:cd09886    1 SWYALRTNPGCEQRAEEALEARGVEA---------FLPMLTEERKRRRKKFDVERPLFPGYVFARLdRSKGQDTSTIRAC 71

                         90       100
                 ....*....|....*....|....*.
gi 493445302  91 AKVTGFVGGARnRPTPISPKEVEKIM 116
Cdd:cd09886   72 DGVLGVVGFDG-RPAPVPEQEMRDLR 96
NGN_SP_RfaH cd09892
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), RfaH; ...
 12-116 5.18e-06

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), RfaH; RfaH is an operon-specific virulence regulator, thought to have arisen from an early duplication of N-Utilization Substance G (NusG). Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination in bacteria. In contrast, RfaH is a non-essential protein that controls expression of operons containing an ops (operon polarity suppressor) element in their transcribed DNA. RfaH and NusG are different in their response to Rho-dependent terminators and regulatory targets. The NusG N-terminal (NGN) domain is quite similar in all NusG orthologs, but its C-terminal domains and the linker that separate these two domains are different. The domain organization of NusG and its homologs suggest that the common properties of NusG and RfaH are due to their similar NGN domains.


Pssm-ID: 193581 [Multi-domain]  Cd Length: 96  Bit Score: 43.32  E-value: 5.18e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  12 RWYVVHAYSGMEKsvqRAlQERIERAGMQdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKNTA 91
Cdd:cd09892    1 AWYLLYTKPRQEE---RA-AENLERQGFE-----VFLPMIRVEKRRRGKRTVVTEPLFPGYLFVRLDPEVQNWRPIRSTR 71

                         90       100
                 ....*....|....*....|....*
gi 493445302  92 KVTGFVGGArNRPTPISPKEVEKIM 116
Cdd:cd09892   72 GVSRLVRFG-GEPAPVPDALIEALR 95
NGN_SP_TaA cd09893
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), TaA; ...
 12-116 7.95e-06

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), TaA; The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination. NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination in bacteria. Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS biosynthesis genes. NusG SP family members are operon-specific transcriptional antiterminationn factors. TaA is a NusG SP factor that is required for synthesis of a polyketide antibiotic TA in Myxococcus xanthus. Orthologs of the NusG gene exist in all bacteria, but its functions and requirements are different. The NusG N-terminal (NGN) domain is quite similar in all NusG orthologs, but its C-terminal domains and the linker that separate these two domains are different. The domain organization of NusG and its orthologs suggest that the common properties of NusG and its orthologs and paralogs are due to their similar NGN domains.


Pssm-ID: 193582 [Multi-domain]  Cd Length: 95  Bit Score: 42.68  E-value: 7.95e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  12 RWYVVHAYSGMEKSVQRALQER-IEragmqdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETWHLVKnT 90
Cdd:cd09893    1 SWYALYTRSRHEKKVADRLAKKgIE----------SFLPLYEVLSRWKDRKKKIKVPLFPGYLFVRFQLDPERLRILK-T 69

                         90       100
                 ....*....|....*....|....*.
gi 493445302  91 AKVTGFVGGaRNRPTPISPKEVEKIM 116
Cdd:cd09893   70 PGVVRIVGN-SGGPIPIPDEEIASLR 94
NGN_SP_UpxY cd09895
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), UpxY; ...
 12-116 6.86e-05

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), UpxY; The N-Utilization Substance G (NusG) proteins are involved in transcription elongation and termination. NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination. Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS (lipopolysaccharide) biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. UpxY proteins, UpxY proteins, where the x is replaced by the letter designation of the specific polysaccharide (UpaY to UphY), are a family of NusG SP factors that act specifically in transcriptional antitermination of operons from which they are encoded. UpxYs are necessary and specific for transcription regulation of the polysaccharide biosynthesis operon. Orthologs of the NusG gene exist in all bacteria, but their functions and requirements are different. The NusG N-terminal (NGN) domain is similar in all NusG orthologs, but its C-terminal domain and the linker that separate these two domains are different. The domain organization of NusG and its orthologs suggests that the common properties of NusG and its orthologs and paralogs are due to their similar NGN domains.


Pssm-ID: 193584 [Multi-domain]  Cd Length: 95  Bit Score: 39.87  E-value: 6.86e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  12 RWYVVHAYSGMEKSVQRALQER-IEragmqdkfgqILVPTEEVVEVKGGHKAVTERRFFPGYVLVEMEMTDETwhLVKNT 90
Cdd:cd09895    1 PWYALYTFPRREKKVAEYLEKKgIE----------CFLPMQYEVRQWSGRKKRVEVPLFPNLVFVHITREELD--EVLET 68

                         90       100
                 ....*....|....*....|....*.
gi 493445302  91 AKVTGFVGGARNRPTPISPKEVEKIM 116
Cdd:cd09895   69 PGVVRFVRYRGKEPAIIPDKQMESFR 94
KOW pfam00467
KOW motif; This family has been extended to coincide with ref. The KOW (Kyprides, Ouzounis, ...
 134-164 1.22e-04

KOW motif; This family has been extended to coincide with ref. The KOW (Kyprides, Ouzounis, Woese) motif is found in a variety of ribosomal proteins and NusG.


Pssm-ID: 425698 [Multi-domain]  Cd Length: 32  Bit Score: 37.75  E-value: 1.22e-04
                          10        20        30
                  ....*....|....*....|....*....|.
gi 493445302  134 VGEMVRVKEGPFTDFNGTVEEVNYEKSRVRV 164
Cdd:pfam00467   1 KGDVVRVIAGPFKGKVGKVVEVDDKKNRVLV 31
nusG PRK08559
transcription antitermination protein NusG; Validated
 6-166 1.48e-04

transcription antitermination protein NusG; Validated


Pssm-ID: 181467 [Multi-domain]  Cd Length: 153  Bit Score: 40.24  E-value: 1.48e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302   6 ASPSGKRWYVVHAYSGMEKSVQRALQERIERAGMQDKfgQILVPTEevveVKGghkavterrffpgYVLVEMEMTDETWH 85
Cdd:PRK08559   1 MKPEMSMIFAVKTTAGQERNVALMLAMRAKKENLPIY--AILAPPE----LKG-------------YVLVEAESKGAVEE 61

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 493445302  86 LVKNTAKVTGFVGGarnrptPISPKEVEKIMSQMQEGVEkprpktlFEVGEMVRVKEGPFTDFNGTVEEVNYEKSRVRVS 165
Cdd:PRK08559  62 AIRGIPHVRGVVPG------EISFEEVEHFLKPKPIVEG-------IKEGDIVELIAGPFKGEKARVVRVDESKEEVTVE 128


                 .
gi 493445302 166 V 166
Cdd:PRK08559 129 L 129
KOW smart00739
KOW (Kyprides, Ouzounis, Woese) motif; Motif in ribosomal proteins, NusG, Spt5p, KIN17 and T54.
 131-158 7.94e-04

KOW (Kyprides, Ouzounis, Woese) motif; Motif in ribosomal proteins, NusG, Spt5p, KIN17 and T54.


Pssm-ID: 128978  Cd Length: 28  Bit Score: 35.38  E-value: 7.94e-04
                           10        20
                   ....*....|....*....|....*...
gi 493445302   131 LFEVGEMVRVKEGPFTDFNGTVEEVNYE 158
Cdd:smart00739   1 KFEVGDTVRVIAGPFKGKVGKVLEVDGE 28
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH