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Conserved domains on  [gi|489185868|ref|WP_003095288|]
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MULTISPECIES: glycine cleavage system protein H [Pseudomonas]

Protein Classification

glycine cleavage system protein H( domain architecture ID 10785558)

glycine cleavage system protein H plays a role in the degradation of glycine by shuttling the methylamine group of glycine from P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase).

EC:  1.4.1.27
Gene Ontology:  GO:0019464|GO:0009249|GO:0005960
PubMed:  20375021|18941301

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 9-137 3.17e-41

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


:

Pssm-ID: 440275  Cd Length: 128  Bit Score: 134.10  E-value: 3.17e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489185868   9 EFPDGLFYAPDYnLWLREEVDGSLTLGLSAYGCALYGEIFAFTPKRDGWHIERDRSFGVVEFAKAASSARSPLAGTLLAS 88
Cdd:COG0509    2 EIPDDLKYTEDH-EWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEV 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 489185868  89 NPAVVKRPALINRDCYGEGWMVRLQPDDWaSARGAFLQGDAAREAFAER 137
Cdd:COG0509   81 NEALEDDPELVNEDPYGEGWLFKIKPSDP-AELDDLLDAEAYAALLEEE 128
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 9-137 3.17e-41

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 134.10  E-value: 3.17e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489185868   9 EFPDGLFYAPDYnLWLREEVDGSLTLGLSAYGCALYGEIFAFTPKRDGWHIERDRSFGVVEFAKAASSARSPLAGTLLAS 88
Cdd:COG0509    2 EIPDDLKYTEDH-EWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEV 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 489185868  89 NPAVVKRPALINRDCYGEGWMVRLQPDDWaSARGAFLQGDAAREAFAER 137
Cdd:COG0509   81 NEALEDDPELVNEDPYGEGWLFKIKPSDP-AELDDLLDAEAYAALLEEE 128
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 16-112 1.67e-32

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 111.09  E-value: 1.67e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489185868  16 YAPDYnLWLREEVDGSLTLGLSAYGCALYGEIFAFTPKRDGWHIERDRSFGVVEFAKAASSARSPLAGTLLASNPAVVKR 95
Cdd:cd06848    1 YTKDH-EWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDN 79

                         90
                 ....*....|....*..
gi 489185868  96 PALINRDCYGEGWMVRL 112
Cdd:cd06848   80 PELINSDPYGEGWLVKI 96
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 3-136 9.11e-31

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 108.17  E-value: 9.11e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489185868   3 ANLHGLEFPDGLFYAPDYNLWLREEVDGSLTLGLSAYGCALYGEIFAFTPKRDGWHIERDRSFGVVEFAKAASSARSPLA 82
Cdd:PRK13380   2 VVENGCEIPSELRYTIKNHEWLRLEGDGTVTVGITDYAQTMAGDVVFVRLKELGKKVEKGKPVATLESGKWAGPVPAPLT 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 489185868  83 GTLLASNPAVVKRPALINRDCYGEGWMVRLQPDDwASARGAFLQGDAAREAFAE 136
Cdd:PRK13380  82 GEVVEVNEALEDSPELVNEDPYGEGWFFRFKPAN-PEELKQLLDGDAADRLLKE 134
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 23-116 2.05e-25

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 93.55  E-value: 2.05e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489185868   23 WLREEVDGSLTLGLSAYGCALYGEIFAFTPKRDGWHIERDRSFGVVEFAKAASSARSPLAGTLLASNPAVVKRPALINRD 102
Cdd:pfam01597   9 WVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLEDNPGLINKD 88

                          90
                  ....*....|....
gi 489185868  103 CYGEGWMVRLQPDD 116
Cdd:pfam01597  89 PYEDGWIAKLKPSN 102
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 9-137 3.17e-41

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 134.10  E-value: 3.17e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489185868   9 EFPDGLFYAPDYnLWLREEVDGSLTLGLSAYGCALYGEIFAFTPKRDGWHIERDRSFGVVEFAKAASSARSPLAGTLLAS 88
Cdd:COG0509    2 EIPDDLKYTEDH-EWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEV 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 489185868  89 NPAVVKRPALINRDCYGEGWMVRLQPDDWaSARGAFLQGDAAREAFAER 137
Cdd:COG0509   81 NEALEDDPELVNEDPYGEGWLFKIKPSDP-AELDDLLDAEAYAALLEEE 128
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 16-112 1.67e-32

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 111.09  E-value: 1.67e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489185868  16 YAPDYnLWLREEVDGSLTLGLSAYGCALYGEIFAFTPKRDGWHIERDRSFGVVEFAKAASSARSPLAGTLLASNPAVVKR 95
Cdd:cd06848    1 YTKDH-EWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDN 79

                         90
                 ....*....|....*..
gi 489185868  96 PALINRDCYGEGWMVRL 112
Cdd:cd06848   80 PELINSDPYGEGWLVKI 96
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 3-136 9.11e-31

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 108.17  E-value: 9.11e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489185868   3 ANLHGLEFPDGLFYAPDYNLWLREEVDGSLTLGLSAYGCALYGEIFAFTPKRDGWHIERDRSFGVVEFAKAASSARSPLA 82
Cdd:PRK13380   2 VVENGCEIPSELRYTIKNHEWLRLEGDGTVTVGITDYAQTMAGDVVFVRLKELGKKVEKGKPVATLESGKWAGPVPAPLT 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 489185868  83 GTLLASNPAVVKRPALINRDCYGEGWMVRLQPDDwASARGAFLQGDAAREAFAE 136
Cdd:PRK13380  82 GEVVEVNEALEDSPELVNEDPYGEGWFFRFKPAN-PEELKQLLDGDAADRLLKE 134
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 23-116 2.05e-25

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 93.55  E-value: 2.05e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489185868   23 WLREEVDGSLTLGLSAYGCALYGEIFAFTPKRDGWHIERDRSFGVVEFAKAASSARSPLAGTLLASNPAVVKRPALINRD 102
Cdd:pfam01597   9 WVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLEDNPGLINKD 88

                          90
                  ....*....|....
gi 489185868  103 CYGEGWMVRLQPDD 116
Cdd:pfam01597  89 PYEDGWIAKLKPSN 102
PRK01202 PRK01202
glycine cleavage system protein GcvH;
9-136 1.21e-23

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 89.45  E-value: 1.21e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489185868   9 EFPDGLFYAPDYNlWLREEvDGSLTLGLSAYGCALYGEI-FAFTPKRdGWHIERDRSFGVVEFAKAASSARSPLAGTLLA 87
Cdd:PRK01202   3 NIPADLKYTKSHE-WVRVE-GDTATVGITDHAQEQLGDIvFVELPEV-GDEVKAGETFGVVESVKAASDIYAPVSGEVVE 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 489185868  88 SNPAVVKRPALINRDCYGEGWMVRLQPDDwASARGAFLQGDAAREAFAE 136
Cdd:PRK01202  80 VNEALEDSPELVNEDPYGEGWLFKIKPSD-ESELDDLLDAEAYEALIEE 127
PRK00624 PRK00624
glycine cleavage system protein H; Provisional
 16-121 2.86e-04

glycine cleavage system protein H; Provisional


Pssm-ID: 167014  Cd Length: 114  Bit Score: 38.27  E-value: 2.86e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489185868  16 YAPDYNLWLREEVDGSLTLGLSAYGCALYGEIFAFTPKRDGWHIERDRSFGVVEFAKAASSARSPLAGTLLASNPAVVKR 95
Cdd:PRK00624   3 WYSDYHVWIEPIHSRIVRLGLTSKMQENLGNILHIDLPSVGSFCKEGEVLVILESSKSAIEVLSPVSGEVIEVNTALEDD 82

                         90       100
                 ....*....|....*....|....*..
gi 489185868  96 PALINRDCYGEGWMVRLQPD-DWASAR 121
Cdd:PRK00624  83 IQPINNAPESEGWFVVVQLDeDFDSEN 109
GT4_Bme6-like cd03821
Brucella melitensis Bme6 and similar proteins; This family is most closely related to the GT4 ...
 96-139 9.28e-03

Brucella melitensis Bme6 and similar proteins; This family is most closely related to the GT4 family of glycosyltransferases. Bme6 in Brucella melitensis has been shown to be involved in the biosynthesis of a polysaccharide.


Pssm-ID: 340848 [Multi-domain]  Cd Length: 377  Bit Score: 35.42  E-value: 9.28e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 489185868  96 PALINRDC-------YGEGWMVRLQPDDWASARGAFLQGDAAREAFAERMR 139
Cdd:cd03821  307 PVVITDKCglselveAGCGVVVDPNVSSLAEALAEALRDPADRKRLGEMAR 357
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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