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Conserved domains on  [gi|488401035|ref|WP_002470420|]
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MULTISPECIES: UDP-N-acetylmuramate--L-alanine ligase [Staphylococcus]

Protein Classification

UDP-N-acetylmuramate--L-alanine ligase( domain architecture ID 11433731)

UDP-N-acetylmuramate--L-alanine ligase catalyzes the addition of the first amino acid to the cytoplasmic precursor of the bacterial cell wall peptidoglycan

CATH:  3.90.190.20|3.40.1190.10
EC:  6.3.2.8
Gene Ontology:  GO:0008763|GO:0009252|GO:0071555|GO:0051301|GO:0007049|GO:0005524|GO:0008360
PubMed:  28201974|16934839
SCOP:  4000154

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MurC COG0773
UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall ...
 1-423 0e+00

UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family is part of the Pathway/BioSystem: Mureine biosynthesis


:

Pssm-ID: 440536 [Multi-domain]  Cd Length: 451  Bit Score: 535.03  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035   1 MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIESYVFTEvALRNKGIKI-LPFDANNITKEMVVIQGNAFPDNHEEIVR 79
Cdd:COG0773    4 IMHIHFIGIGGIGMSGLAEILLALGYKVSGSDLAESPMTE-RLEALGIPVfIGHDAENIDDADLVVVSSAIPRDNPELVA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  80 ADELKLDIIKYHDFLGHVINQYTSVAVTGAHGKTSTTGLLSHVMN-GDKKTSFLIGD-----GTGMGLPGSDYFAFEACE 153
Cdd:COG0773   83 ARERGIPVLSRAEMLAELMRGKRSIAVAGTHGKTTTTSMLAHILEeAGLDPTFLIGGilnnfGTNARLGDGDYFVAEADE 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 154 YRRHFLSYHPDYAIMTNIDFDHPDYFKNIDDVYDAFQHMALNVKKG--IIAWGDDEYLRKL--DVDIPVYYYGFKETDDI 229
Cdd:COG0773  163 SDGSFLHYSPDIAVVTNIEADHLDIYGDLEAIKEAFHEFARNVPFYglLVLCADDPGLRELlpRCGRPVITYGFSEDADY 242

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 230 YAKNIQITEKGTQFDVYIKGEFYDQFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNETKVSNQVL 309
Cdd:COG0773  243 RAENIRIDGGGSTFDVLRRGEELGEVELNLPGRHNVLNALAAIAVALELGVDPEAIAEALASFKGVKRRFELKGEVGGVT 322

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 310 V-DDYAHHPREISATIETARKKYPKKDVVAVFQPHTFSRTQAFLNEFAESLSKADQVFLCEIFGSIRENTGDLTIEDLIN 388
Cdd:COG0773  323 ViDDYAHHPTEIAATLAAAREKYPDRRLVAVFQPHRYSRTRDFLDEFAEALSLADEVILLDIYAAREKPIPGVSSEDLAE 402

                        410       420       430       440
                 ....*....|....*....|....*....|....*....|...
gi 488401035 389 RI--DGSTLIDENSIDVLEKF------DNAVILFMGAGDIQKL 423
Cdd:COG0773  403 AIrkRGKDVVYVPDLDELVEAlaeiarPGDVVLTMGAGDIGGL 445
 
Name Accession Description Interval E-value
MurC COG0773
UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall ...
 1-423 0e+00

UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440536 [Multi-domain]  Cd Length: 451  Bit Score: 535.03  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035   1 MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIESYVFTEvALRNKGIKI-LPFDANNITKEMVVIQGNAFPDNHEEIVR 79
Cdd:COG0773    4 IMHIHFIGIGGIGMSGLAEILLALGYKVSGSDLAESPMTE-RLEALGIPVfIGHDAENIDDADLVVVSSAIPRDNPELVA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  80 ADELKLDIIKYHDFLGHVINQYTSVAVTGAHGKTSTTGLLSHVMN-GDKKTSFLIGD-----GTGMGLPGSDYFAFEACE 153
Cdd:COG0773   83 ARERGIPVLSRAEMLAELMRGKRSIAVAGTHGKTTTTSMLAHILEeAGLDPTFLIGGilnnfGTNARLGDGDYFVAEADE 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 154 YRRHFLSYHPDYAIMTNIDFDHPDYFKNIDDVYDAFQHMALNVKKG--IIAWGDDEYLRKL--DVDIPVYYYGFKETDDI 229
Cdd:COG0773  163 SDGSFLHYSPDIAVVTNIEADHLDIYGDLEAIKEAFHEFARNVPFYglLVLCADDPGLRELlpRCGRPVITYGFSEDADY 242

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 230 YAKNIQITEKGTQFDVYIKGEFYDQFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNETKVSNQVL 309
Cdd:COG0773  243 RAENIRIDGGGSTFDVLRRGEELGEVELNLPGRHNVLNALAAIAVALELGVDPEAIAEALASFKGVKRRFELKGEVGGVT 322

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 310 V-DDYAHHPREISATIETARKKYPKKDVVAVFQPHTFSRTQAFLNEFAESLSKADQVFLCEIFGSIRENTGDLTIEDLIN 388
Cdd:COG0773  323 ViDDYAHHPTEIAATLAAAREKYPDRRLVAVFQPHRYSRTRDFLDEFAEALSLADEVILLDIYAAREKPIPGVSSEDLAE 402

                        410       420       430       440
                 ....*....|....*....|....*....|....*....|...
gi 488401035 389 RI--DGSTLIDENSIDVLEKF------DNAVILFMGAGDIQKL 423
Cdd:COG0773  403 AIrkRGKDVVYVPDLDELVEAlaeiarPGDVVLTMGAGDIGGL 445
murC TIGR01082
UDP-N-acetylmuramate--L-alanine ligase; This model describes the MurC protein in bacterial ...
 3-423 2.64e-146

UDP-N-acetylmuramate--L-alanine ligase; This model describes the MurC protein in bacterial peptidoglycan (murein) biosynthesis. In a few species (Mycobacterium leprae, the Chlamydia), the amino acid may be L-serine or glycine instead of L-alanine. A related protein, UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (murein tripeptide ligase) is described by model TIGR01081. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273433 [Multi-domain]  Cd Length: 448  Bit Score: 424.03  E-value: 2.64e-146
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035    3 HYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIESyVFTEVALRNKGIKIL-PFDANNITKEMVVIQGNAFPDNHEEIVRAD 81
Cdd:TIGR01082   1 KIHFVGIGGIGMSGIAEILLNRGYQVSGSDIAE-NATTKRLEALGIPIYiGHSAENLDDADVVVVSAAIKDDNPEIVEAK 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035   82 ELKLDIIKYHDFLGHVINQYTSVAVTGAHGKTSTTGLLSHVMNGDKK-TSFLIGD-----GTGMGLPGSDYFAFEACEYR 155
Cdd:TIGR01082  80 ERGIPVIRRAEMLAELMRFRHSIAVAGTHGKTTTTAMIAVILKEAGLdPTVVVGGlvkeaGTNARLGSGEYLVAEADESD 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  156 RHFLSYHPDYAIMTNIDFDHPD-YFKNIDDVYDAFQHMALNVKKG--IIAWGDDEYLRKLDVDI---PVYYYGFKETDDI 229
Cdd:TIGR01082 160 ASFLHLQPNVAIVTNIEPDHLDtYGSSFERLKAAFEKFIHNLPFYglAVICADDPVLRELVPKAteqVITYGGSGEDADY 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  230 YAKNIQITEKGTQFDVYIKGEFYDQFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNET-KVSNQV 308
Cdd:TIGR01082 240 RAENIQQSGAEGKFSVRGKGKLYLEFTLNLPGRHNVLNALAAIAVALELGIDFEAILRALANFQGVKRRFEILgEFGGVL 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  309 LVDDYAHHPREISATIETARKKYPKKDVVAVFQPHTFSRTQAFLNEFAESLSKADQVFLCEIFGSIRENTGDLTIEDLIN 388
Cdd:TIGR01082 320 LIDDYAHHPTEIKATLKAARQGYPDKRIVVVFQPHRYSRTRDLFDDFAKVLSDADELILLDIYAAGEEPINGIDGKSLAR 399

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....
gi 488401035  389 RIDGSTLIDENSIDVLEKFDNA---------VILFMGAGDIQKL 423
Cdd:TIGR01082 400 KITQLGKIEPYFVPDLAELVEFlaavlqsgdLILTMGAGDIIKL 443
PRK14573 PRK14573
bifunctional UDP-N-acetylmuramate--L-alanine ligase/D-alanine--D-alanine ligase;
3-423 2.06e-59

bifunctional UDP-N-acetylmuramate--L-alanine ligase/D-alanine--D-alanine ligase;


Pssm-ID: 184752 [Multi-domain]  Cd Length: 809  Bit Score: 207.75  E-value: 2.06e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035   3 HYHFVGIKGSGMSSLAQIMHDLGHEVQGSDI-ESYVFTEvaLRNKGIKILP-FDANNITKEMVVIQGNAFPDNHEEIVRA 80
Cdd:PRK14573   6 FYHFIGIGGIGMSALAHILLDRGYSVSGSDLsEGKTVEK--LKAKGARFFLgHQEEHVPEDAVVVYSSSISKDNVEYLSA 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  81 DELKLDIIKYHDFLGHVINQYTSVAVTGAHGKTSTTGLLSHVMNGDKKT-SFLIGDGTGMGLPG----SDYFAFEACEYR 155
Cdd:PRK14573  84 KSRGNRLVHRAELLAELMQEQISILVSGSHGKTTVSSLITAIFQEAKKDpSYAIGGLNQEGLNGysgsSEYFVAEADESD 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 156 RHFLSYHPDYAIMTNIDFDHPDYFK-NIDDVYDAFQHMALNVKKGIIAW--GDDEYLRKldvDIPVYYYGFKETDDIYAk 232
Cdd:PRK14573 164 GSLKHYTPEFSVITNIDNEHLSNFEgDRELLLASIQDFARKVQQINKCFynGDCPRLKG---CLQGHSYGFSSSCDLHI- 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 233 nIQITEKGTQ--FDVYIKGEFYDQFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNETKVSNQVL- 309
Cdd:PRK14573 240 -LSYYQEGWRsyFSAKFLGVVYQDIELNLVGMHNVANAAAAMGIALTLGIDEGAIRNALKGFSGVQRRLERKNSSETFLf 318

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 310 VDDYAHHPREISATIETARKKYPKKDVVAVFQPHTFSRTQAFLNEFAESLSKADQVFLCEIFGSIRENTGDLTIEDLINR 389
Cdd:PRK14573 319 LEDYAHHPSEISCTLRAVRDAVGLRRIIAICQPHRFSRLRECLDSFPSAFQDADEVILTDVYSAGEEPEDSISYQKLAEA 398

                        410       420       430       440
                 ....*....|....*....|....*....|....*....|..
gi 488401035 390 IDGSTLID------ENSIDVLEKFDNA--VILFMGAGDIQKL 423
Cdd:PRK14573 399 ISQSSIVKctyvpfHEIQRYLEQSIRVhdVCVSLGAGNIYTL 440
Mur_ligase_M pfam08245
Mur ligase middle domain;
106-274 3.49e-25

Mur ligase middle domain;


Pssm-ID: 462409 [Multi-domain]  Cd Length: 199  Bit Score: 102.00  E-value: 3.49e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  106 VTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTG---------MGLP---------GSDYFAFEACEYRR--HFLSYH--P 163
Cdd:pfam08245   1 VTGTNGKTTTTELIAAILSLAGGVIGTIGTYIGksgnttnnaIGLPltlaemveaGAEYAVLEVSSHGLgeGRLSGLlkP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  164 DYAIMTNIDFDHPDYFKNIDDVYDAFQHMALNVKKGIIA--WGDDEYLRKL-----DVDIPVYYYGFKETDDIYAKNIQI 236
Cdd:pfam08245  81 DIAVFTNISPDHLDFHGTMENYAKAKAELFEGLPEDGIAviNADDPYGAFLiaklkKAGVRVITYGIEGEADLRAANIEL 160

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 488401035  237 TEKGTQFDVYIKGEFYDQFLSPQYGNHNILNALAVIAI 274
Cdd:pfam08245 161 SSDGTSFDLFTVPGGELEIEIPLLGRHNVYNALAAIAA 198
 
Name Accession Description Interval E-value
MurC COG0773
UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall ...
 1-423 0e+00

UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramate-alanine ligase MurC and related ligases, MurC/Mpl family is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440536 [Multi-domain]  Cd Length: 451  Bit Score: 535.03  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035   1 MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIESYVFTEvALRNKGIKI-LPFDANNITKEMVVIQGNAFPDNHEEIVR 79
Cdd:COG0773    4 IMHIHFIGIGGIGMSGLAEILLALGYKVSGSDLAESPMTE-RLEALGIPVfIGHDAENIDDADLVVVSSAIPRDNPELVA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  80 ADELKLDIIKYHDFLGHVINQYTSVAVTGAHGKTSTTGLLSHVMN-GDKKTSFLIGD-----GTGMGLPGSDYFAFEACE 153
Cdd:COG0773   83 ARERGIPVLSRAEMLAELMRGKRSIAVAGTHGKTTTTSMLAHILEeAGLDPTFLIGGilnnfGTNARLGDGDYFVAEADE 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 154 YRRHFLSYHPDYAIMTNIDFDHPDYFKNIDDVYDAFQHMALNVKKG--IIAWGDDEYLRKL--DVDIPVYYYGFKETDDI 229
Cdd:COG0773  163 SDGSFLHYSPDIAVVTNIEADHLDIYGDLEAIKEAFHEFARNVPFYglLVLCADDPGLRELlpRCGRPVITYGFSEDADY 242

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 230 YAKNIQITEKGTQFDVYIKGEFYDQFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNETKVSNQVL 309
Cdd:COG0773  243 RAENIRIDGGGSTFDVLRRGEELGEVELNLPGRHNVLNALAAIAVALELGVDPEAIAEALASFKGVKRRFELKGEVGGVT 322

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 310 V-DDYAHHPREISATIETARKKYPKKDVVAVFQPHTFSRTQAFLNEFAESLSKADQVFLCEIFGSIRENTGDLTIEDLIN 388
Cdd:COG0773  323 ViDDYAHHPTEIAATLAAAREKYPDRRLVAVFQPHRYSRTRDFLDEFAEALSLADEVILLDIYAAREKPIPGVSSEDLAE 402

                        410       420       430       440
                 ....*....|....*....|....*....|....*....|...
gi 488401035 389 RI--DGSTLIDENSIDVLEKF------DNAVILFMGAGDIQKL 423
Cdd:COG0773  403 AIrkRGKDVVYVPDLDELVEAlaeiarPGDVVLTMGAGDIGGL 445
murC TIGR01082
UDP-N-acetylmuramate--L-alanine ligase; This model describes the MurC protein in bacterial ...
 3-423 2.64e-146

UDP-N-acetylmuramate--L-alanine ligase; This model describes the MurC protein in bacterial peptidoglycan (murein) biosynthesis. In a few species (Mycobacterium leprae, the Chlamydia), the amino acid may be L-serine or glycine instead of L-alanine. A related protein, UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (murein tripeptide ligase) is described by model TIGR01081. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273433 [Multi-domain]  Cd Length: 448  Bit Score: 424.03  E-value: 2.64e-146
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035    3 HYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIESyVFTEVALRNKGIKIL-PFDANNITKEMVVIQGNAFPDNHEEIVRAD 81
Cdd:TIGR01082   1 KIHFVGIGGIGMSGIAEILLNRGYQVSGSDIAE-NATTKRLEALGIPIYiGHSAENLDDADVVVVSAAIKDDNPEIVEAK 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035   82 ELKLDIIKYHDFLGHVINQYTSVAVTGAHGKTSTTGLLSHVMNGDKK-TSFLIGD-----GTGMGLPGSDYFAFEACEYR 155
Cdd:TIGR01082  80 ERGIPVIRRAEMLAELMRFRHSIAVAGTHGKTTTTAMIAVILKEAGLdPTVVVGGlvkeaGTNARLGSGEYLVAEADESD 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  156 RHFLSYHPDYAIMTNIDFDHPD-YFKNIDDVYDAFQHMALNVKKG--IIAWGDDEYLRKLDVDI---PVYYYGFKETDDI 229
Cdd:TIGR01082 160 ASFLHLQPNVAIVTNIEPDHLDtYGSSFERLKAAFEKFIHNLPFYglAVICADDPVLRELVPKAteqVITYGGSGEDADY 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  230 YAKNIQITEKGTQFDVYIKGEFYDQFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNET-KVSNQV 308
Cdd:TIGR01082 240 RAENIQQSGAEGKFSVRGKGKLYLEFTLNLPGRHNVLNALAAIAVALELGIDFEAILRALANFQGVKRRFEILgEFGGVL 319

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  309 LVDDYAHHPREISATIETARKKYPKKDVVAVFQPHTFSRTQAFLNEFAESLSKADQVFLCEIFGSIRENTGDLTIEDLIN 388
Cdd:TIGR01082 320 LIDDYAHHPTEIKATLKAARQGYPDKRIVVVFQPHRYSRTRDLFDDFAKVLSDADELILLDIYAAGEEPINGIDGKSLAR 399

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....
gi 488401035  389 RIDGSTLIDENSIDVLEKFDNA---------VILFMGAGDIQKL 423
Cdd:TIGR01082 400 KITQLGKIEPYFVPDLAELVEFlaavlqsgdLILTMGAGDIIKL 443
PRK14573 PRK14573
bifunctional UDP-N-acetylmuramate--L-alanine ligase/D-alanine--D-alanine ligase;
3-423 2.06e-59

bifunctional UDP-N-acetylmuramate--L-alanine ligase/D-alanine--D-alanine ligase;


Pssm-ID: 184752 [Multi-domain]  Cd Length: 809  Bit Score: 207.75  E-value: 2.06e-59
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035   3 HYHFVGIKGSGMSSLAQIMHDLGHEVQGSDI-ESYVFTEvaLRNKGIKILP-FDANNITKEMVVIQGNAFPDNHEEIVRA 80
Cdd:PRK14573   6 FYHFIGIGGIGMSALAHILLDRGYSVSGSDLsEGKTVEK--LKAKGARFFLgHQEEHVPEDAVVVYSSSISKDNVEYLSA 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  81 DELKLDIIKYHDFLGHVINQYTSVAVTGAHGKTSTTGLLSHVMNGDKKT-SFLIGDGTGMGLPG----SDYFAFEACEYR 155
Cdd:PRK14573  84 KSRGNRLVHRAELLAELMQEQISILVSGSHGKTTVSSLITAIFQEAKKDpSYAIGGLNQEGLNGysgsSEYFVAEADESD 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 156 RHFLSYHPDYAIMTNIDFDHPDYFK-NIDDVYDAFQHMALNVKKGIIAW--GDDEYLRKldvDIPVYYYGFKETDDIYAk 232
Cdd:PRK14573 164 GSLKHYTPEFSVITNIDNEHLSNFEgDRELLLASIQDFARKVQQINKCFynGDCPRLKG---CLQGHSYGFSSSCDLHI- 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 233 nIQITEKGTQ--FDVYIKGEFYDQFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNETKVSNQVL- 309
Cdd:PRK14573 240 -LSYYQEGWRsyFSAKFLGVVYQDIELNLVGMHNVANAAAAMGIALTLGIDEGAIRNALKGFSGVQRRLERKNSSETFLf 318

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 310 VDDYAHHPREISATIETARKKYPKKDVVAVFQPHTFSRTQAFLNEFAESLSKADQVFLCEIFGSIRENTGDLTIEDLINR 389
Cdd:PRK14573 319 LEDYAHHPSEISCTLRAVRDAVGLRRIIAICQPHRFSRLRECLDSFPSAFQDADEVILTDVYSAGEEPEDSISYQKLAEA 398

                        410       420       430       440
                 ....*....|....*....|....*....|....*....|..
gi 488401035 390 IDGSTLID------ENSIDVLEKFDNA--VILFMGAGDIQKL 423
Cdd:PRK14573 399 ISQSSIVKctyvpfHEIQRYLEQSIRVhdVCVSLGAGNIYTL 440
mpl TIGR01081
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase; Alternate name: ...
 3-367 4.23e-48

UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase; Alternate name: murein tripeptide ligase [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 130153 [Multi-domain]  Cd Length: 448  Bit Score: 170.40  E-value: 4.23e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035    3 HYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIESYVFTEVALRNKGIKILP-FDANNIT-KEMVVIQGNAFPDNHEEIVRA 80
Cdd:TIGR01081   1 HIHILGICGTFMGGLAMIAKQLGHEVTGSDANVYPPMSTQLEAQGIEIIEgFDAAQLEpKPDLVVIGNAMKRGNPCVEAV 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035   81 DELKLDIIKYHDFLG-HVINQYTSVAVTGAHGKTSTTGLLSHVM--NGdKKTSFLIGD-----GTGMGLPGSDYFAFEAC 152
Cdd:TIGR01081  81 LNLNLPYTSGPQWLHdFVLHDRWVLAVAGTHGKTTTASMLAWVLeqCG-LKPGFLIGGvpgnfGVSARLGESPFFVIEAD 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  153 EY-------RRHFLSYHPDYAIMTNIDFDHPDYFKNIDDVYDAFQHMALNV-KKGIIAWGDDEYLRKLDVDI----PVYY 220
Cdd:TIGR01081 160 EYdtaffdkRSKFVHYRPRTLVLNNLEFDHADIFDDLKAIQRQFHHLVRTVpGEGLILCPGRDQSLKDTLAKgcwsEQEF 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  221 YGfkETDDIYAKniQITEKGTQFDVYIKGEFYDQFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFN 300
Cdd:TIGR01081 240 FG--EQGEWQAE--KITADGSHFDVLLDGEKVGEVKWSLVGRHNMHNALMAIAAARHVGVAIEDACEALGSFVNAKRRLE 315

                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 488401035  301 ETKVSNQVLV-DDYAHHPREISATIETARKKYPKKDVVAVFQPHTFSRTQAFLNE-FAESLSKADQVFL 367
Cdd:TIGR01081 316 LKGEANGITVyDDFAHHPTAIEATLQGLRQKVGGARILAVLEPRSNTMKLGVHKDdLAPSLGRADQVFL 384
MurE COG0769
UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 104-340 2.73e-30

UDP-N-acetylmuramyl tripeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl tripeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440532 [Multi-domain]  Cd Length: 459  Bit Score: 121.72  E-value: 2.73e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 104 VAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGdGTGMGLP--------------------------GSDYFAFE----ACE 153
Cdd:COG0769   83 IGVTGTNGKTTTTYLLAQILRALGKKTGLIG-TVGNGIGgelipsslttpealdlqrllaemvdaGVTHVVMEvsshALD 161

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 154 YRR----HFlsyhpDYAIMTNIDFDHPDYFKNIDDVYDA----FQhMALNVKKGIIaWGDDEYLRKL--DVDIPVYYYGF 223
Cdd:COG0769  162 QGRvdgvRF-----DVAVFTNLTRDHLDYHGTMEAYFAAkarlFD-QLGPGGAAVI-NADDPYGRRLaaAAPARVITYGL 234

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 224 KETDDIYAKNIQITEKGTQFDVYIKGEFYDqFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNETK 303
Cdd:COG0769  235 KADADLRATDIELSADGTRFTLVTPGGEVE-VRLPLIGRFNVYNALAAIAAALALGIDLEEILAALEKLKGVPGRMERVD 313

                        250       260       270
                 ....*....|....*....|....*....|....*...
gi 488401035 304 VSNQVLV-DDYAHHPREISATIETARkKYPKKDVVAVF 340
Cdd:COG0769  314 GGQGPTViVDYAHTPDALENVLEALR-PHTKGRLIVVF 350
Mur_ligase_M pfam08245
Mur ligase middle domain;
106-274 3.49e-25

Mur ligase middle domain;


Pssm-ID: 462409 [Multi-domain]  Cd Length: 199  Bit Score: 102.00  E-value: 3.49e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  106 VTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTG---------MGLP---------GSDYFAFEACEYRR--HFLSYH--P 163
Cdd:pfam08245   1 VTGTNGKTTTTELIAAILSLAGGVIGTIGTYIGksgnttnnaIGLPltlaemveaGAEYAVLEVSSHGLgeGRLSGLlkP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  164 DYAIMTNIDFDHPDYFKNIDDVYDAFQHMALNVKKGIIA--WGDDEYLRKL-----DVDIPVYYYGFKETDDIYAKNIQI 236
Cdd:pfam08245  81 DIAVFTNISPDHLDFHGTMENYAKAKAELFEGLPEDGIAviNADDPYGAFLiaklkKAGVRVITYGIEGEADLRAANIEL 160

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 488401035  237 TEKGTQFDVYIKGEFYDQFLSPQYGNHNILNALAVIAI 274
Cdd:pfam08245 161 SSDGTSFDLFTVPGGELEIEIPLLGRHNVYNALAAIAA 198
MurF COG0770
UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; ...
 104-312 1.47e-23

UDP-N-acetylmuramyl pentapeptide synthase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramyl pentapeptide synthase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440533 [Multi-domain]  Cd Length: 451  Bit Score: 102.49  E-value: 1.47e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 104 VAVTGAHGKTSTTGLLSHVMNGDKKT-----SF--LIGdgtgmgLP--------GSDYFAFEA-----------CEYRRh 157
Cdd:COG0770  103 IAITGSNGKTTTKEMLAAVLSTKGKVlatpgNFnnEIG------VPltllrlpeDHEFAVLEMgmnhpgeiaylARIAR- 175

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 158 flsyhPDYAIMTNIDFDHPDYFKNIDDVYDA----FQHMAlnvKKGI-IAWGDDEYLRKL--DVDIPVYYYGFKETDDIY 230
Cdd:COG0770  176 -----PDIAVITNIGPAHLEGFGSLEGIARAkgeiFEGLP---PGGVaVLNADDPLLAALaeRAKARVLTFGLSEDADVR 247

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 231 AKNIQITEKGTQFDVYIKGEFYDqFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNETKVSNQVLV 310
Cdd:COG0770  248 AEDIELDEDGTRFTLHTPGGELE-VTLPLPGRHNVSNALAAAAVALALGLDLEEIAAGLAAFQPVKGRLEVIEGAGGVTL 326


                 ...
gi 488401035 311 -DD 312
Cdd:COG0770  327 iDD 329
murE PRK00139
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional
 93-340 1.32e-18

UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase; Provisional


Pssm-ID: 234660 [Multi-domain]  Cd Length: 460  Bit Score: 87.49  E-value: 1.32e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  93 FLGHVINQYTSVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGdGTGMGLP--------------------------GSDY 146
Cdd:PRK00139  87 FYGHPSDKLKLIGVTGTNGKTTTAYLLAQILRLLGEKTALIG-TLGNGIGgelipsglttpdaldlqrllaelvdaGVTY 165

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 147 FAFE----ACEYRR----HFlsyhpDYAIMTNIDFDHPDYFKNIDDVYDA----FQHMALnvkKGIIaWGDDEYLRKLDV 214
Cdd:PRK00139 166 AAMEvsshALDQGRvdglKF-----DVAVFTNLSRDHLDYHGTMEDYLAAkarlFSELGL---AAVI-NADDEVGRRLLA 236

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 215 DIPVYYYGFKETDdIYAKNIQITEKGTQFDvyikgeFYDQFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGG 294
Cdd:PRK00139 237 LPDAYAVSMAGAD-LRATDVEYTDSGQTFT------LVTEVESPLIGRFNVSNLLAALAALLALGVPLEDALAALAKLQG 309

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*...
gi 488401035 295 VKRRFNETKVSNQ--VLVdDYAHHPREISATIETARkKYPKKDVVAVF 340
Cdd:PRK00139 310 VPGRMERVDAGQGplVIV-DYAHTPDALEKVLEALR-PHAKGRLICVF 355
MurD COG0771
UDP-N-acetylmuramoylalanine-D-glutamate ligase [Cell wall/membrane/envelope biogenesis]; ...
 7-299 2.46e-18

UDP-N-acetylmuramoylalanine-D-glutamate ligase [Cell wall/membrane/envelope biogenesis]; UDP-N-acetylmuramoylalanine-D-glutamate ligase is part of the Pathway/BioSystem: Mureine biosynthesis


Pssm-ID: 440534 [Multi-domain]  Cd Length: 445  Bit Score: 86.67  E-value: 2.46e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035   7 VGIKGSGMSsLAQIMHDLGHEVQGSDI-ESYVFTEVALRNKGIKIL--PFDANNITK-EMVVIqgN-AFPDNHEEIVRAD 81
Cdd:COG0771   10 LGLGKSGLA-AARLLAKLGAEVTVSDDrPAPELAAAELEAPGVEVVlgEHPEELLDGaDLVVK--SpGIPPDHPLLKAAR 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  82 ELKLDII-------KYHDflGHVInqytsvAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGD-GTG-----MGLPGSDYFA 148
Cdd:COG0771   87 AAGIPVIgeielayRLSP--APII------AITGTNGKTTTTTLIGHILKAAGLRVAVGGNiGTPlldllLEPEPPDVYV 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 149 FEaceyrrhfLS---------YHPDYAIMTNIDFDHPDYFKNIDDVYDAFQHMALNVKKGIIA--WGDDEYLRKL--DVD 215
Cdd:COG0771  159 LE--------LSsfqlettpsLRPDVAVILNITPDHLDRHGSMEAYAAAKARIFANQTPDDYAvlNADDPLTRALaeEAK 230

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 216 IPVYYYGFKE--TDDIYAKNIQITEKGTQF------DVYIKGEfydqflspqygnHNILNALAVIAISYLENMNVENIKE 287
Cdd:COG0771  231 ARVVPFSLKEplEGGAGLEDGKLVDRASGEellpvdDLRLPGR------------HNLENALAALAAARALGVPPEAIRE 298

                        330
                 ....*....|..
gi 488401035 288 ALITFGGVKRRF 299
Cdd:COG0771  299 ALRSFKGLPHRL 310
murD TIGR01087
UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation ...
 7-312 3.13e-18

UDP-N-acetylmuramoylalanine--D-glutamate ligase; [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273436 [Multi-domain]  Cd Length: 433  Bit Score: 86.24  E-value: 3.13e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035    7 VGIKGSGMS--SLAQIMHDLGHEVQGSDIESYVFTEVALRN--------KGIKILPFDANNITkemVVIQGNAFPDNHEE 76
Cdd:TIGR01087   2 ILILGLGKTgrAVARFLHKKGAEVTVTDLKPNEELEPSMGQlrlnegsvLHTGLHLEDLNNAD---LVVKSPGIPPDHPL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035   77 IVRADELKLDIIKYHDFLGHvINQYTSVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGD-GT----GMGLPGSDYFAFEA 151
Cdd:TIGR01087  79 VQAAAKRGIPVVGDIELFLR-LVPLPVVAITGTNGKTTTTSLLYHLLKAAGLKAFLGGNiGTpaleVLDQEGAELYVLEL 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  152 CEYRRHFL-SYHPDYAIMTNIDFDHPDYFKNIDDVYDAFQHMALNVKKG--IIAWGDDEYLRKLDVDIPVYYYGFKETDD 228
Cdd:TIGR01087 158 SSFQLETTeSLRPEIALILNISEDHLDWHGSFEDYVAAKLKIFARQTEGdvAVLNADDPRFARLAQKSKAQVIWFSVEKD 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  229 IYAKNIQITEKgtqfdVYIKGEFYDQFLSPQygnHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNETKVSNQV 308
Cdd:TIGR01087 238 AERGLCIRDGG-----LYLKPNDLEGSLLGL---HNAENILAAIALAKSLGLNLEAILEALRSFKGLPHRLEYVGQKNGV 309


                  ....*
gi 488401035  309 L-VDD 312
Cdd:TIGR01087 310 HfYND 314
Mur_ligase pfam01225
Mur ligase family, catalytic domain; This family contains a number of related ligase enzymes ...
 3-102 1.09e-17

Mur ligase family, catalytic domain; This family contains a number of related ligase enzymes which have EC numbers 6.3.2.*. This family includes: MurC, MurD, MurE, MurF, Mpl and FolC. MurC, MurD, Mure and MurF catalyze consecutive steps in the synthesis of peptidoglycan. Peptidoglycan consists of a sheet of two sugar derivatives, with one of these N-acetylmuramic acid attaching to a small pentapeptide. The pentapeptide is is made of L-alanine, D-glutamic acid, Meso-diaminopimelic acid and D-alanyl alanine. The peptide moiety is synthesized by successively adding these amino acids to UDP-N-acetylmuramic acid. MurC transfers the L-alanine, MurD transfers the D-glutamate, MurE transfers the diaminopimelic acid, and MurF transfers the D-alanyl alanine. This family also includes Folylpolyglutamate synthase that transfers glutamate to folylpolyglutamate.


Pssm-ID: 460121 [Multi-domain]  Cd Length: 84  Bit Score: 77.27  E-value: 1.09e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035    3 HYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIESYvftevaLRNKGIKILPFDAnnitkemvviQGNAFPDNHEEIVRADE 82
Cdd:pfam01225   1 EIHFVGIDGRGMSPGALFLALKGYRVDGSDFIES------LIALGAAAVVGHD----------AANNISPDNPELEAAKV 64

                          90       100
                  ....*....|....*....|
gi 488401035   83 LKLDIIKYHDFLGHVINQYT 102
Cdd:pfam01225  65 PGIPVIDRREALAELAAAFY 84
murE TIGR01085
UDP-N-acetylmuramyl-tripeptide synthetase; Most members of this family are EC 6.3.2.13, ...
 93-340 1.77e-16

UDP-N-acetylmuramyl-tripeptide synthetase; Most members of this family are EC 6.3.2.13, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase. An exception is Staphylococcus aureus, in which diaminopimelate is replaced by lysine in the peptidoglycan and MurE is EC 6.3.2.7. The Mycobacteria, part of the closest neighboring branch outside of the low-GC Gram-positive bacteria, use diaminopimelate. A close homolog, scoring just below the trusted cutoff, is found (with introns) in Arabidopsis thaliana. Its role is unknown. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273435 [Multi-domain]  Cd Length: 464  Bit Score: 81.21  E-value: 1.77e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035   93 FLGHVINQYTSVAVTGAHGKTSTTGLLSHVMN-GDKKTSFL------IGDGTGMGLP---------------------GS 144
Cdd:TIGR01085  77 FYGHPSKKLKVIGVTGTNGKTTTTSLIAQLLRlLGKKTGLIgtigyrLGGNDLIKNPaalttpealtlqstlaemveaGA 156

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  145 DYFAFEAC-----EYRRHFLSYhpDYAIMTNIDFDHPDYFKNIDDVYDAFQHM--ALNVKKGIIAWGDDEY----LRKLD 213
Cdd:TIGR01085 157 QYAVMEVSshalaQGRVRGVRF--DAAVFTNLSRDHLDFHGTMENYFAAKASLftELGLKRFAVINLDDEYgaqfVKRLP 234

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  214 VDIPVYY---YGFKETDDIYAKNIQITEKGTQFDV-YIKGEFydQFLSPQYGNHNILNALAVIAI-SYLENMNVENIKEA 288
Cdd:TIGR01085 235 KDITVSAitqPADGRAQDIKITDSGYSFEGQQFTFeTPAGEG--HLHTPLIGRFNVYNLLAALATlLHLGGIDLEDIVAA 312

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 488401035  289 LITFGGVKRRF----NETKVSnqVLVdDYAHHPREISATIETARKKYPKKdVVAVF 340
Cdd:TIGR01085 313 LEKFRGVPGRMelvdGGQKFL--VIV-DYAHTPDALEKALRTLRKHKDGR-LIVVF 364
murF TIGR01143
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the ...
 104-313 2.85e-16

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; This family consists of the strictly bacterial MurF gene of peptidoglycan biosynthesis. This enzyme is almost always UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanyl ligase, but in a few species, MurE adds lysine rather than diaminopimelate. This enzyme acts on the product from MurE activity, and so is also subfamily rather than equivalog. Staphylococcus aureus is an example of species in this MurF protein would differ. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]


Pssm-ID: 273468 [Multi-domain]  Cd Length: 417  Bit Score: 80.39  E-value: 2.85e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  104 VAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGD-GTGMGLPGS--------DYFAFEACEYRRHFLSY-----HPDYAIMT 169
Cdd:TIGR01143  77 IGITGSSGKTTTKEMLAAILSHKYKVFATPGNfNNEIGLPLTllrapgdhDYAVLEMGASHPGEIAYlaeiaKPDIAVIT 156

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  170 NIDFDHPDYFKNIDDV-------YDAFQHMALNVKKGIIAWGDdeYLRKLDVDIPVYYYGFkETDDIYAKNIQITEKG-T 241
Cdd:TIGR01143 157 NIGPAHLEGFGSLEGIaeakgeiLQGLKENGIAVINADDPAFA--DLAKRLPNRNILSFGF-EGGDFVAKDISYSALGsT 233

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 488401035  242 QFDVYIKGEFYDQFLsPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNETKVSNQVLVDDY 313
Cdd:TIGR01143 234 SFTLVAPGGEFEVSL-PLLGRHNVMNALAAAALALELGIPLEEIAEGLAELKLVKGRFEVQTKNGLTLIDDT 304
Mur_ligase_C pfam02875
Mur ligase family, glutamate ligase domain; This family contains a number of related ligase ...
 295-381 5.27e-14

Mur ligase family, glutamate ligase domain; This family contains a number of related ligase enzymes which have EC numbers 6.3.2.*. This family includes: MurC, MurD, MurE, MurF, Mpl and FolC. MurC, MurD, Mure and MurF catalyze consecutive steps in the synthesis of peptidoglycan. Peptidoglycan consists of a sheet of two sugar derivatives, with one of these N-acetylmuramic acid attaching to a small pentapeptide. The pentapeptide is is made of L-alanine, D-glutamic acid, Meso-diaminopimelic acid and D-alanyl alanine. The peptide moiety is synthesized by successively adding these amino acids to UDP-N-acetylmuramic acid. MurC transfers the L-alanine, MurD transfers the D-glutamate, MurE transfers the diaminopimelic acid, and MurF transfers the D-alanyl alanine. This family also includes Folylpolyglutamate synthase that transfers glutamate to folylpolyglutamate.


Pssm-ID: 460731 [Multi-domain]  Cd Length: 87  Bit Score: 66.98  E-value: 5.27e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  295 VKRRFNETKVSNQVLV-DDYAHHPREISATIETARKKYPKKdVVAVFQP--HtfsRTQAFLNEFAESLSK-ADQVFLCEI 370
Cdd:pfam02875   1 VPGRLEVVGENNGVLViDDYAHNPDAMEAALRALRNLFPGR-LILVFGGmgD---RDAEFHALLGRLAAAlADVVILTGD 76

                          90
                  ....*....|.
gi 488401035  371 FGSIRENTGDL 381
Cdd:pfam02875  77 YPRAEDPGAII 87
PRK11929 PRK11929
bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE ...
 92-340 4.60e-10

bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF;


Pssm-ID: 237025 [Multi-domain]  Cd Length: 958  Bit Score: 62.03  E-value: 4.60e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035  92 DFLGHVINQYTSVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIG------DGTGMGLP-------------------GSDY 146
Cdd:PRK11929 103 RWYGRPSEQLSLVAVTGTNGKTSCAQLLAQLLTRLGKPCGSIGtlgarlDGRLIPGSlttpdaiilhrilarmraaGADA 182

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 147 FAFEACEyrrHFLSYHP------DYAIMTNIDFDHPDYFKNIDDVYDA----------FQHMALNVkkgiiawgDDEYLR 210
Cdd:PRK11929 183 VAMEASS---HGLEQGRldglriAVAGFTNLTRDHLDYHGTMQDYEEAkaalfsklpgLGAAVINA--------DDPAAA 251

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 211 KLDVDIP----VYYYGFKETDDIYAKNIQITEKGTQFDVYIKGEFYdQFLSPQYGNHNILNALAVIAISYLENMNVENIK 286
Cdd:PRK11929 252 RLLAALPrglkVGYSPQNAGADVQARDLRATAHGQVFTLATPDGSY-QLVTRLLGRFNVSNLLLVAAALKKLGLPLAQIA 330

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 287 EALITFGGVKRRF----NETKVSNQVLVDDYAHHPREISATIETAR--KKYPKKDVVAVF 340
Cdd:PRK11929 331 RALAAVSPVPGRMervgPTAGAQGPLVVVDYAHTPDALAKALTALRpvAQARNGRLVCVF 390
murD PRK14106
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; Provisional
 104-298 2.90e-07

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; Provisional


Pssm-ID: 184511 [Multi-domain]  Cd Length: 450  Bit Score: 52.28  E-value: 2.90e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 104 VAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDgtgMGLPGSDY----------------FAFEACEYrrhflsYHPDYAI 167
Cdd:PRK14106 111 VAITGTNGKTTTTTLLGEIFKNAGRKTLVAGN---IGYPLIDAveeygeddiivaevssFQLETIKE------FKPKVGC 181

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 168 MTNIDFDHPDYFKNIDDVYDAFQHMALNVKKG---IIAWgDDEYLRKLDVDIP--VYYYGFKE--TDDIYAKN--IQITE 238
Cdd:PRK14106 182 ILNITPDHLDRHKTMENYIKAKARIFENQRPSdytVLNY-DDPRTRSLAKKAKarVIFFSRKSllEEGVFVKNgkIVISL 260

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 239 KGTQFDVYIKGEFYDQflspqyGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRR 298
Cdd:PRK14106 261 GGKEEEVIDIDEIFIP------GEHNLENALAATAAAYLLGISPDVIANTLKTFKGVEHR 314
PRK11929 PRK11929
bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE ...
 104-326 1.78e-06

bifunctional UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase MurE/UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF;


Pssm-ID: 237025 [Multi-domain]  Cd Length: 958  Bit Score: 50.47  E-value: 1.78e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 104 VAVTGAHGKTSTTGLLSHVM------NGDKKT--SF--LIG---------DGT-------GMGLPGS-DYFAFEAceyrr 156
Cdd:PRK11929 606 VAITGSNGKTTTKEMIAAILaawqgeDRVLATegNFnnEIGvpltllrlrAQHraavfelGMNHPGEiAYLAAIA----- 680

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 157 hflsyHPDYAIMTNIDFDHPDYFKNIDDVYDAFQHMALNVKKGIIAW--GDDEYL---RKLDVDIPVYYYGFKETDDIYA 231
Cdd:PRK11929 681 -----APTVALVTNAQREHQEFMHSVEAVARAKGEIIAALPEDGVAVvnGDDPYTaiwAKLAGARRVLRFGLQPGADVYA 755

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 232 ----KNIQITEKG-TQFDVYIKGEFYDQFLsPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNETKVSN 306
Cdd:PRK11929 756 ekiaKDISVGEAGgTRCQVVTPAGSAEVYL-PLIGEHNLRNALAAIACALAAGASLKQIRAGLERFQPVAGRMQRRRLSC 834

                        250       260
                 ....*....|....*....|..
gi 488401035 307 QV-LVDD-YAHHPREISATIET 326
Cdd:PRK11929 835 GTrIIDDtYNANPDSMRAAIDV 856
murF PRK10773
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; Reviewed
 228-312 3.75e-04

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; Reviewed


Pssm-ID: 182718 [Multi-domain]  Cd Length: 453  Bit Score: 42.71  E-value: 3.75e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488401035 228 DIYAKNIQITEKGTQFDVYI-KGEFydQFLSPQYGNHNILNALAVIAISYLENMNVENIKEALITFGGVKRRFNETKVS- 305
Cdd:PRK10773 248 DFTATNIHVTSHGTEFTLHTpTGSV--DVLLPLPGRHNIANALAAAALAMSVGATLDAVKAGLANLKAVPGRLFPIQLAe 325


                 ....*..
gi 488401035 306 NQVLVDD 312
Cdd:PRK10773 326 GQLLLDD 332
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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